CN208609718U - A kind of device for adopting ovum and larva raising for citrus fruit fly - Google Patents

A kind of device for adopting ovum and larva raising for citrus fruit fly Download PDF

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Publication number
CN208609718U
CN208609718U CN201821272343.3U CN201821272343U CN208609718U CN 208609718 U CN208609718 U CN 208609718U CN 201821272343 U CN201821272343 U CN 201821272343U CN 208609718 U CN208609718 U CN 208609718U
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China
Prior art keywords
ovum
citrus fruit
fruit fly
larva raising
box body
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CN201821272343.3U
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Chinese (zh)
Inventor
袁瑞玲
陈鹏
郑传伟
冯丹
王艺璇
杜春花
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Yunnan Academy of Forestry
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Yunnan Academy of Forestry
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Abstract

The utility model discloses a kind of device that ovum and larva raising are adopted for citrus fruit fly in insect breeding apparatus technical field, including box body, box cover and tocostome, the upper end of the box body is connected with box cover, four side walls of the box body are uniformly provided with tocostome respectively, distance is 0.5~1cm between two adjacent tocostomes, the utility model can be the biology of citrus fruit fly, physiology, behaviouristics, science of heredity, it is ecological, especially it is big to provide quantity for the further investigation in Prevention Technique field, development is neat, the good worm sources material of physiological status consistency, and citrus fruit fly adopts ovum and larva raising apparatus structure is simple, it can be recycled, it is low in cost, it occupies little space, facilitate biological control, saving manpower easy to operate.

Description

A kind of device for adopting ovum and larva raising for citrus fruit fly
Technical field
The utility model relates to insect breeding apparatus technical field, specific field adopts ovum and children for citrus fruit fly to be a kind of The device of worm raising.
Background technique
Citrus fruit fly also known as orient fruit fly, are subordinate to Tephritidae, and Bactrocera is to endanger citrus, guava, mango etc. More than 250 important pests with Important Economic value melon and fruit, because of its kind flight, life cycle is short, host is extensive, reproductive capacity By force, harmfulness is big, referred to as " fruits and vegetables killer ", is classified as important quarantine pest by countries in the world always;Citrus fruit fly adult Ovum is originated under pericarp, feeding pulp, brill moth cause harm in fruit after larvae hatch, cause pulp to rot, fruit is caused to lose food With value, the yield and quality of the tropical fruit (tree)s such as mango and vegetables is seriously affected, huge economic loss is caused, to citrus fruit fly Progress biology, physiology, behaviouristics, science of heredity, ecology, the especially further investigation in Prevention Technique field become emphasis, Currently, with molecular biology fast development and reach its maturity, utilize RNAi, the technique for gene engineerings pest control such as transgenosis As the hot spot of control of insect technical research, it is required to that sufficient stabilization, growth and development be neat, physiology shape when carrying out the studies above The consistent worm sources of condition;
Existing raising citrus fruit fly method is that the tropical fruit (tree)s such as banana are placed in dependent insect cage, and female adult produces ovum in fruit Breeding, fruit are easy to rot, and smell unbearably, generates a large amount of drosophila, pollute laboratory environment, while raising out citrus fruit fly hair Educate different, the purpose of this utility model is to provide a kind of device that ovum and larva raising are adopted for citrus fruit fly, which can raise It is big to raise quantity, develops neat, the good citrus fruit fly of physiological status consistency, is biology, physiology, the row of citrus fruit fly Sufficient worm sources material is provided for the further investigation in, science of heredity, ecology, especially Prevention Technique field.
Utility model content
The purpose of this utility model is to provide a kind of devices that ovum and larva raising are adopted for citrus fruit fly, on solving State the problem of proposing in background technique.
To achieve the above object, the utility model provides the following technical solutions: one kind adopting ovum and larva for citrus fruit fly The device of raising, including box body, the upper end of the box body are connected with box cover, and four side walls of the box body are uniformly provided with production respectively Micropyle, distance is 0.5~1cm between two adjacent tocostomes.
Preferably, the box body is transparent plastic box, and the size of the box body is 17cm × 11.5cm × 6.7cm, wall thickness 0.35mm。
Preferably, the aperture of the tocostome is 0.6mm, the production of lower side on four side walls of the box body The bottom surface of micropyle and box body distance is at least 1.5cm.
Compared with prior art, the utility model has the beneficial effects that
1, a kind of device that ovum and larva raising are adopted for citrus fruit fly of the utility model, be citrus fruit fly biology, It is big that the further investigation in physiology, behaviouristics, science of heredity, ecology, especially Prevention Technique field provides quantity, development is neat, The good worm sources material of physiological status consistency, and citrus fruit fly is adopted that ovum and larva raising apparatus structure are simple, can be recycled, It is low in cost, occupy little space, facilitate biological control, saving manpower easy to operate.
2, a kind of device that ovum and larva raising are adopted for citrus fruit fly of the utility model, citrus fruit fly oviposition is lured, The collection of ovum is brought together with hatching together with larva raising, is realized and is adopted the seamless connection of ovum to larva raising: same Citrus fruit fly, which is adopted, to be added after mango juice lures citrus fruit fly Adult worms producting eggs in ovum and larva raising device, and artificial larva is directly added Ovum around tocostome is sticked on feed using artificial larvae feeds and mango juice blend, is admixed in feed to it by feed Then worm's ovum is transferred to the device equipped with larvae feeds in contrast to worm's ovum is obtained by washing and filtering in the prior art by hatching Middle carry out larva raising, the utility model can collect all ovum grains completely, effectively improve worm's ovum yield, while reduce other behaviour Make the mechanical wounding to ovum, guarantee that citrus fruit fly development is neat, physiological status consistency is good, effectively improve the hatching rate of ovum, Reach prolific purpose.
3, a kind of device that ovum and larva raising are adopted for citrus fruit fly of the utility model, using rectangle transparent plastic box Ovum and larva raising device are adopted as citrus fruit fly, is developed to the mature larva stage in newly hatched larvae, box cover is conducive to artificial children Worm feed moisturizing, tocostome are conducive to limited air circulation, and box body is transparent to be convenient for breeding observing, and box body is square can be up and down Close-packed arrays save the usable floor area of growth cabinet, are convenient for biological control, open the lid, citrus fruit fly is adopted ovum and children Worm breeding apparatus is put into the container equipped with suitable humidity fine sand, and spontaneous jump out of citrus fruit fly mature larva pierces in fine sand at this time It pupates, it is easy to operate, save manpower.
4, citrus fruit fly is carried out oviposition and drawn by a kind of device that ovum and larva raising are adopted for citrus fruit fly of the utility model Lure, the collection of ovum and hatching be brought together together with larva raising, citrus fruit fly adopts box body and box in ovum and larva raising device It is in clamped condition when covering long, therefore has evaded common laboratory rearing citrus fruit fly by placing the heat such as banana in dependent insect cage Ovum is produced and is bred in fruit by band fruit, female adult, and fruit rots and gives out bad smell and generate a large amount of drosophila, and pollution is real The phenomenon that testing room environmental.
Detailed description of the invention
Fig. 1 is the utility model agent structure schematic diagram.
In figure: 1- box body, 2- box cover, 3- tocostome.
Specific embodiment
Below in conjunction with the utility model embodiment, the technical solution of the utility model is clearly and completely described, Obviously, the described embodiments are only a part of the embodiments of the utility model, instead of all the embodiments.It is practical based on this Embodiment in novel, every other reality obtained by those of ordinary skill in the art without making creative efforts Example is applied, is fallen within the protection scope of the utility model.
Embodiment 1:
Referring to Fig. 1, producing citrus fruit fly first adopts ovum and larva raising device, box body 1 uses transparent plastic box, box The size of body is 17cm × 11.5cm × 6.7cm, and wall thickness 0.35mm, the upper end of box body is connected with box cover 2, four side walls of box body Tocostome 3 is uniformly opened respectively, and tocostome aperture is 0.6mm, and distance is 0.5cm between two adjacent tocostomes, is located at box The bottom surface distance of the tocostome and box body of lower side is 1.5cm on four side walls of body.
Then it carries out citrus fruit fly and adopts ovum and larva raising
1) oviposition is lured
Mango juice is added to citrus fruit fly to adopt in ovum and larva raising device, mango juice adopts ovum and larva in citrus fruit fly It is 1cm that depth is added in breeding apparatus, after addition, covers tightly box cover, citrus fruit fly is adopted ovum and larva raising device is put into In the rearing-box of the citrus fruit fly in egg-laying season, for citrus fruit fly Adult worms producting eggs, ovum is adopted to citrus fruit fly daily and larva raising fills It is primary to set replacement;
Randomly select 4 citrus fruit flies in step 1) and adopt ovum and larva raising device, each citrus fruit fly adopt ovum and The length and width face of larva raising device is each to select 5 tocostome statistics ovum grain number amounts at random, is shown in Table 1
1 citrus fruit fly of table adopts ovum and larva raising device acquisition ovum grain number amount
Artificial larvae feeds production:
By bean dregs 45g, corn flour 4g, soybean powder 3g, sucrose 14g, yeast powder 7g, methyl p-hydroxybenzoate 0.3g, vinegar Sour 3ml is added in 20ml distilled water and is uniformly mixed, and mixture is carried out microwave heating using preceding, heating time 1min adds It is taken out after heat, cooled to room temperature can be used.
2) collection and hatching of ovum
Citrus fruit fly after Adult worms producting eggs in step 1) is adopted ovum and larva raising device to take out, to citrus fruit fly adopt ovum and Artificial larvae feeds are added in larva raising device, then stir evenly artificial larvae feeds and mango juice, reuse spoon Son provokes artificial larvae feeds and mango juice blend adopts the inner wall of ovum and larva raising device along citrus fruit fly, will be attached to interior Worm's ovum on wall is viscous lower and admixes in artificial larvae feeds and mango juice blend, and citrus fruit fly is then adopted ovum and larva raising Device is put into growth cabinet, and growth cabinet is set as 27 DEG C of temperature, humidity 65%, photoperiod 16L:8D;
3) larva raising
The citrus fruit fly larvae development of the egg hatching in ovum and larva raising device is adopted to aging to step 2) citrus fruit fly When larva, citrus fruit fly is adopted into ovum and larva raising device takes out from growth cabinet, opens the lid and is put into equipped with temperature 25 DEG C, in the container of the fine sand of humidity 70%, citrus fruit fly mature larva is spontaneous to be jumped out to pierce in fine sand and pupates.
4 citrus fruit flies are randomly selected in step 2) and adopt ovum and larva raising device, count the hatching of bactrocera dorsalis ovum Rate and larvae pupation rate, are shown in Table 2
Wherein: hatching rate=hatch larva number/test ovum number × 100%
Larva number × 100% that percentage of pupation=number/egg hatching of pupating goes out
The hatching rate and larvae pupation rate of 2 bactrocera dorsalis ovum of table
Embodiment 2:
Referring to Fig. 1, producing citrus fruit fly first adopts ovum and larva raising device, box body 1 uses transparent plastic box, box The size of body is 17cm × 11.5cm × 6.7cm, and wall thickness 0.35mm, the upper end of box body is connected with box cover 2, four side walls of box body Tocostome 3 is uniformly opened respectively, and tocostome aperture is 0.6mm, and distance is 0.8cm between two adjacent tocostomes, is located at box The bottom surface distance of the tocostome and box body of lower side is 1.8cm on four side walls of body.
Then it carries out citrus fruit fly and adopts ovum and larva raising
1) oviposition is lured
Mango juice is added to citrus fruit fly to adopt in ovum and larva raising device, mango juice adopts ovum and larva in citrus fruit fly It is 1.2cm that depth is added in breeding apparatus, after addition, covers tightly box cover, citrus fruit fly is adopted ovum and larva raising device is put In the rearing-box for entering the citrus fruit fly in egg-laying season, for citrus fruit fly Adult worms producting eggs, ovum and larva raising are adopted to citrus fruit fly daily Device replacement is primary;
Randomly select 4 citrus fruit flies in step 1) and adopt ovum and larva raising device, each citrus fruit fly adopt ovum and The length and width face of larva raising device is each to select 5 tocostome statistics ovum grain number amounts at random, is shown in Table 1
1 citrus fruit fly of table adopts ovum and larva raising device acquisition ovum grain number amount
Artificial larvae feeds production:
By bean dregs 45g, corn flour 4g, soybean powder 3g, sucrose 14g, yeast powder 7g, methyl p-hydroxybenzoate 0.3g, vinegar Sour 4ml is added in 22ml distilled water and is uniformly mixed, and mixture is carried out microwave heating using preceding, heating time 1.5min, It is taken out after heating, cooled to room temperature can be used.
2) collection and hatching of ovum
Citrus fruit fly after Adult worms producting eggs in step 1) is adopted ovum and larva raising device to take out, to citrus fruit fly adopt ovum and Artificial larvae feeds are added in larva raising device, then stir evenly artificial larvae feeds and mango juice, reuse spoon Son provokes artificial larvae feeds and mango juice blend adopts the inner wall of ovum and larva raising device along citrus fruit fly, will be attached to interior Worm's ovum on wall is viscous lower and admixes in artificial larvae feeds and mango juice blend, and citrus fruit fly is then adopted ovum and larva raising Device is put into growth cabinet, and growth cabinet is set as 28 DEG C of temperature, humidity 70%, photoperiod 16L:8D;
3) larva raising
The citrus fruit fly larvae development of the egg hatching in ovum and larva raising device is adopted to aging to step 2) citrus fruit fly When larva, citrus fruit fly is adopted into ovum and larva raising device takes out from growth cabinet, opens the lid and is put into equipped with temperature 25 DEG C, in the container of the fine sand of humidity 70%, citrus fruit fly mature larva is spontaneous to be jumped out to pierce in fine sand and pupates.
4 citrus fruit flies are randomly selected in step 2) and adopt ovum and larva raising device, count the hatching of bactrocera dorsalis ovum Rate and larvae pupation rate, are shown in Table 2
Wherein: hatching rate=hatch larva number/test ovum number × 100%
Larva number × 100% that percentage of pupation=number/egg hatching of pupating goes out
The hatching rate and larvae pupation rate of 2 bactrocera dorsalis ovum of table
Embodiment 3:
Referring to Fig. 1, producing citrus fruit fly first adopts ovum and larva raising device, box body 1 uses transparent plastic box, box The size of body is 17cm × 11.5cm × 6.7cm, and wall thickness 0.35mm, the upper end of box body is connected with box cover 2, four side walls of box body Tocostome 3 is uniformly opened respectively, and tocostome aperture is 0.6mm, and distance is 1cm between two adjacent tocostomes, is located at box body Four side walls on lower side tocostome and box body bottom surface distance be 2cm.
Then it carries out citrus fruit fly and adopts ovum and larva raising
1) oviposition is lured
Mango juice is added to citrus fruit fly to adopt in ovum and larva raising device, mango juice adopts ovum and larva in citrus fruit fly It is 1.5cm that depth is added in breeding apparatus, after addition, covers tightly box cover, citrus fruit fly is adopted ovum and larva raising device is put In the rearing-box for entering the citrus fruit fly in egg-laying season, for citrus fruit fly Adult worms producting eggs, ovum and larva raising are adopted to citrus fruit fly daily Device replacement is primary;
Randomly select 4 citrus fruit flies in step 1) and adopt ovum and larva raising device, each citrus fruit fly adopt ovum and The length and width face of larva raising device is each to select 5 tocostome statistics ovum grain number amounts at random, is shown in Table 1
1 citrus fruit fly of table adopts ovum and larva raising device acquisition ovum grain number amount
Artificial larvae feeds production:
By bean dregs 45g, corn flour 4g, soybean powder 3g, sucrose 14g, yeast powder 7g, methyl p-hydroxybenzoate 0.3g, vinegar Sour 5ml is added in 25ml distilled water and is uniformly mixed, and mixture is carried out microwave heating using preceding, heating time 2min adds It is taken out after heat, cooled to room temperature can be used.
2) collection and hatching of ovum
Citrus fruit fly after Adult worms producting eggs in step 1) is adopted ovum and larva raising device to take out, to citrus fruit fly adopt ovum and Artificial larvae feeds are added in larva raising device, then stir evenly artificial larvae feeds and mango juice, reuse spoon Son provokes artificial larvae feeds and mango juice blend adopts the inner wall of ovum and larva raising device along citrus fruit fly, will be attached to interior Worm's ovum on wall is viscous lower and admixes in artificial larvae feeds and mango juice blend, and citrus fruit fly is then adopted ovum and larva raising Device is put into growth cabinet, and growth cabinet is set as 29 DEG C of temperature, humidity 75%, photoperiod 16L:8D;
3) larva raising
The citrus fruit fly larvae development of the egg hatching in ovum and larva raising device is adopted to aging to step 2) citrus fruit fly When larva, citrus fruit fly is adopted into ovum and larva raising device takes out from growth cabinet, opens the lid and is put into equipped with temperature 25 DEG C, in the container of the fine sand of humidity 70%, citrus fruit fly mature larva is spontaneous to be jumped out to pierce in fine sand and pupates.
4 citrus fruit flies are randomly selected in step 2) and adopt ovum and larva raising device, count the hatching of bactrocera dorsalis ovum Rate and larvae pupation rate, are shown in Table 2
Wherein: hatching rate=hatch larva number/test ovum number × 100%
Larva number × 100% that percentage of pupation=number/egg hatching of pupating goes out
The hatching rate and larvae pupation rate of 2 bactrocera dorsalis ovum of table
A kind of device that ovum and larva raising are adopted for citrus fruit fly of the utility model, in contrast to the prior art, unit plane Product citrus fruit fly raises out that quantity is big, while the hatching rate of citrus fruit fly, percentage of pupation be higher and development is neat, physiological status is consistent Property is good.
While there has been shown and described that the embodiments of the present invention, for the ordinary skill in the art, It is understood that these embodiments can be carried out with a variety of variations in the case where not departing from the principles of the present invention and spirit, repaired Change, replacement and variant, the scope of the utility model is defined by the appended claims and the equivalents thereof.

Claims (3)

1. a kind of device for adopting ovum and larva raising for citrus fruit fly, including box body (1), it is characterised in that: the box body (1) Upper end be connected with box cover (2), four side walls of the box body (1) are uniformly provided with tocostome (3) respectively, described in adjacent two Distance is 0.5~1cm between tocostome (3).
2. a kind of device for adopting ovum and larva raising for citrus fruit fly according to claim 1, it is characterised in that: described Box body (1) is transparent plastic box, and the size of the box body (1) is 17cm × 11.5cm × 6.7cm, wall thickness 0.35mm.
3. a kind of device for adopting ovum and larva raising for citrus fruit fly according to claim 1, it is characterised in that: described The aperture of tocostome (3) be 0.6mm, be located at the box body (1) four side walls on lower side the tocostome (3) with it is described The bottom surface distance of box body (1) is at least 1.5cm.
CN201821272343.3U 2018-08-08 2018-08-08 A kind of device for adopting ovum and larva raising for citrus fruit fly Expired - Fee Related CN208609718U (en)

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Application Number Priority Date Filing Date Title
CN201821272343.3U CN208609718U (en) 2018-08-08 2018-08-08 A kind of device for adopting ovum and larva raising for citrus fruit fly

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
CN201821272343.3U CN208609718U (en) 2018-08-08 2018-08-08 A kind of device for adopting ovum and larva raising for citrus fruit fly

Publications (1)

Publication Number Publication Date
CN208609718U true CN208609718U (en) 2019-03-19

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Granted publication date: 20190319

Termination date: 20190808