CN108835053A - A kind of citrus fruit fly adopts the method and device of ovum and larva raising - Google Patents
A kind of citrus fruit fly adopts the method and device of ovum and larva raising Download PDFInfo
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- CN108835053A CN108835053A CN201810896088.8A CN201810896088A CN108835053A CN 108835053 A CN108835053 A CN 108835053A CN 201810896088 A CN201810896088 A CN 201810896088A CN 108835053 A CN108835053 A CN 108835053A
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- ovum
- citrus fruit
- fruit fly
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- larva
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- 108010000912 Egg Proteins Proteins 0.000 title claims abstract description 134
- 102000002322 Egg Proteins Human genes 0.000 title claims abstract description 134
- 210000004681 ovum Anatomy 0.000 title claims abstract description 134
- 241000255588 Tephritidae Species 0.000 title claims abstract description 133
- 235000020971 citrus fruits Nutrition 0.000 title claims abstract description 132
- 238000000034 method Methods 0.000 title claims abstract description 32
- 238000009395 breeding Methods 0.000 claims abstract description 14
- 230000001488 breeding effect Effects 0.000 claims abstract description 14
- 238000011161 development Methods 0.000 claims abstract description 12
- 235000004936 Bromus mango Nutrition 0.000 claims description 29
- 235000014826 Mangifera indica Nutrition 0.000 claims description 29
- 235000009184 Spondias indica Nutrition 0.000 claims description 29
- 235000011389 fruit/vegetable juice Nutrition 0.000 claims description 27
- 230000012447 hatching Effects 0.000 claims description 26
- 241001093152 Mangifera Species 0.000 claims description 24
- 235000013601 eggs Nutrition 0.000 claims description 19
- 230000012010 growth Effects 0.000 claims description 17
- 239000000203 mixture Substances 0.000 claims description 16
- 230000017448 oviposition Effects 0.000 claims description 12
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- 230000018109 developmental process Effects 0.000 claims description 11
- 239000000843 powder Substances 0.000 claims description 10
- QTBSBXVTEAMEQO-UHFFFAOYSA-N Acetic acid Chemical compound CC(O)=O QTBSBXVTEAMEQO-UHFFFAOYSA-N 0.000 claims description 6
- 229920003023 plastic Polymers 0.000 claims description 6
- 230000002269 spontaneous effect Effects 0.000 claims description 6
- 240000007228 Mangifera indica Species 0.000 claims description 5
- 244000046052 Phaseolus vulgaris Species 0.000 claims description 5
- 235000010627 Phaseolus vulgaris Nutrition 0.000 claims description 5
- 229930006000 Sucrose Natural products 0.000 claims description 5
- CZMRCDWAGMRECN-UGDNZRGBSA-N Sucrose Chemical compound O[C@H]1[C@H](O)[C@@H](CO)O[C@@]1(CO)O[C@@H]1[C@H](O)[C@@H](O)[C@H](O)[C@@H](CO)O1 CZMRCDWAGMRECN-UGDNZRGBSA-N 0.000 claims description 5
- 240000008042 Zea mays Species 0.000 claims description 5
- 235000005824 Zea mays ssp. parviglumis Nutrition 0.000 claims description 5
- 235000002017 Zea mays subsp mays Nutrition 0.000 claims description 5
- 235000005822 corn Nutrition 0.000 claims description 5
- 239000012153 distilled water Substances 0.000 claims description 5
- 235000013312 flour Nutrition 0.000 claims description 5
- 239000004292 methyl p-hydroxybenzoate Substances 0.000 claims description 5
- 235000010270 methyl p-hydroxybenzoate Nutrition 0.000 claims description 5
- LXCFILQKKLGQFO-UHFFFAOYSA-N methylparaben Chemical compound COC(=O)C1=CC=C(O)C=C1 LXCFILQKKLGQFO-UHFFFAOYSA-N 0.000 claims description 5
- 238000003756 stirring Methods 0.000 claims description 5
- 239000005720 sucrose Substances 0.000 claims description 5
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Chemical compound O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 5
- 244000068988 Glycine max Species 0.000 claims description 4
- 235000010469 Glycine max Nutrition 0.000 claims description 4
- 239000002994 raw material Substances 0.000 claims description 2
- 241000238631 Hexapoda Species 0.000 abstract description 5
- 230000002265 prevention Effects 0.000 abstract description 5
- 238000011835 investigation Methods 0.000 abstract description 4
- 239000000463 material Substances 0.000 abstract description 4
- 230000035479 physiological effects, processes and functions Effects 0.000 abstract description 4
- 235000013399 edible fruits Nutrition 0.000 description 10
- 230000019617 pupation Effects 0.000 description 10
- 235000013339 cereals Nutrition 0.000 description 7
- 238000010438 heat treatment Methods 0.000 description 7
- 241001124181 Bactrocera dorsalis Species 0.000 description 6
- 240000004808 Saccharomyces cerevisiae Species 0.000 description 4
- 230000032683 aging Effects 0.000 description 4
- 238000004519 manufacturing process Methods 0.000 description 4
- 238000012360 testing method Methods 0.000 description 3
- 239000000052 vinegar Substances 0.000 description 3
- 235000021419 vinegar Nutrition 0.000 description 3
- 241000675108 Citrus tangerina Species 0.000 description 2
- 241000255581 Drosophila <fruit fly, genus> Species 0.000 description 2
- 240000008790 Musa x paradisiaca Species 0.000 description 2
- 235000018290 Musa x paradisiaca Nutrition 0.000 description 2
- 241000607479 Yersinia pestis Species 0.000 description 2
- 238000012986 modification Methods 0.000 description 2
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- 241001124183 Bactrocera <genus> Species 0.000 description 1
- 241000207199 Citrus Species 0.000 description 1
- 244000241257 Cucumis melo Species 0.000 description 1
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- 241000508269 Psidium Species 0.000 description 1
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- FJJCIZWZNKZHII-UHFFFAOYSA-N [4,6-bis(cyanoamino)-1,3,5-triazin-2-yl]cyanamide Chemical compound N#CNC1=NC(NC#N)=NC(NC#N)=N1 FJJCIZWZNKZHII-UHFFFAOYSA-N 0.000 description 1
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- 230000009368 gene silencing by RNA Effects 0.000 description 1
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- 230000002062 proliferating effect Effects 0.000 description 1
- 108090000623 proteins and genes Proteins 0.000 description 1
- 230000000384 rearing effect Effects 0.000 description 1
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Classifications
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K67/00—Rearing or breeding animals, not otherwise provided for; New or modified breeds of animals
- A01K67/033—Rearing or breeding invertebrates; New breeds of invertebrates
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K10/00—Animal feeding-stuffs
- A23K10/10—Animal feeding-stuffs obtained by microbiological or biochemical processes
- A23K10/16—Addition of microorganisms or extracts thereof, e.g. single-cell proteins, to feeding-stuff compositions
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K10/00—Animal feeding-stuffs
- A23K10/10—Animal feeding-stuffs obtained by microbiological or biochemical processes
- A23K10/16—Addition of microorganisms or extracts thereof, e.g. single-cell proteins, to feeding-stuff compositions
- A23K10/18—Addition of microorganisms or extracts thereof, e.g. single-cell proteins, to feeding-stuff compositions of live microorganisms
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K10/00—Animal feeding-stuffs
- A23K10/30—Animal feeding-stuffs from material of plant origin, e.g. roots, seeds or hay; from material of fungal origin, e.g. mushrooms
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K10/00—Animal feeding-stuffs
- A23K10/30—Animal feeding-stuffs from material of plant origin, e.g. roots, seeds or hay; from material of fungal origin, e.g. mushrooms
- A23K10/37—Animal feeding-stuffs from material of plant origin, e.g. roots, seeds or hay; from material of fungal origin, e.g. mushrooms from waste material
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K20/00—Accessory food factors for animal feeding-stuffs
- A23K20/10—Organic substances
- A23K20/163—Sugars; Polysaccharides
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K50/00—Feeding-stuffs specially adapted for particular animals
- A23K50/90—Feeding-stuffs specially adapted for particular animals for insects, e.g. bees or silkworms
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02P—CLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
- Y02P60/00—Technologies relating to agriculture, livestock or agroalimentary industries
- Y02P60/80—Food processing, e.g. use of renewable energies or variable speed drives in handling, conveying or stacking
- Y02P60/87—Re-use of by-products of food processing for fodder production
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Abstract
The present invention discloses the method and device that a kind of citrus fruit fly in insect method for breeding and breeding apparatus technical field adopts ovum and larva raising, a kind of citrus fruit fly of the present invention adopts the method and device of ovum and larva raising, it can be the biology of citrus fruit fly, physiology, behaviouristics, science of heredity, it is ecological, especially it is big to provide quantity for the further investigation in Prevention Technique field, development is neat, the good worm sources material of physiological status consistency, and the citrus fruit fly being related to adopts ovum and larva raising apparatus structure is simple, it can be recycled, it is low in cost, it occupies little space, facilitate biological control, saving manpower easy to operate.
Description
Technical field
The present invention relates to insect method for breeding and breeding apparatus technical field, specific field be a kind of citrus fruit fly adopt ovum and
The method and device of larva raising.
Background technique
Citrus fruit fly also known as orient fruit fly, are subordinate to Tephritidae, and Bactrocera is to endanger citrus, guava, mango etc.
More than 250 important pests with Important Economic value melon and fruit, because of its kind flight, life cycle is short, host is extensive, reproductive capacity
By force, harmfulness is big, referred to as " fruits and vegetables killer ", is classified as important quarantine pest by countries in the world always；Citrus fruit fly adult
Ovum is originated under pericarp, feeding pulp, brill moth cause harm in fruit after larvae hatch, cause pulp to rot, fruit is caused to lose food
With value, the yield and quality of the tropical fruit (tree)s such as mango and vegetables is seriously affected, huge economic loss is caused, to citrus fruit fly
Progress biology, physiology, behaviouristics, science of heredity, ecology, the especially further investigation in Prevention Technique field become emphasis,
Currently, with molecular biology fast development and reach its maturity, utilize RNAi, the technique for gene engineerings such as transgenosis prevention and treatment evil
Worm become control of insect technical research hot spot, be required to when carrying out the studies above sufficient stabilization, growth and development neatly, physiology
The consistent worm sources of situation；
Existing raising citrus fruit fly method is that the tropical fruit (tree)s such as banana are placed in insect cage, and female adult produces ovum in fruit
Breeding, fruit are easy to rot, and smell unbearably, generates a large amount of drosophila, pollute laboratory environment, while raising out citrus fruit fly hair
Educate it is different, it is an object of the present invention to provide the method and device that a kind of citrus fruit fly adopts ovum and larva raising, the method for breeding and
It is big that device can raise out quantity, and development is neat, the good citrus fruit fly of physiological status consistency, be citrus fruit fly biology,
The further investigation in physiology, behaviouristics, science of heredity, ecology, especially Prevention Technique field provides sufficient worm sources material.
Summary of the invention
It is above-mentioned to solve the purpose of the present invention is to provide the method and device that a kind of citrus fruit fly adopts ovum and larva raising
The problem of being proposed in background technique.
To achieve the above object, the present invention provides the following technical solutions：A kind of citrus fruit fly adopts the side of ovum and larva raising
Method and device, method include the following steps：
1) oviposition is lured
Mango juice is added to citrus fruit fly to adopt in ovum and larva raising device, mango juice adopts ovum and larva in citrus fruit fly
It is 1~1.5cm that depth is added in breeding apparatus, after addition, covers tightly box cover, citrus fruit fly is adopted ovum and larva raising device
It is put into the rearing-box of the citrus fruit fly in egg-laying season, for citrus fruit fly Adult worms producting eggs, ovum is adopted to citrus fruit fly daily and larva is raised
It is primary to support device replacement；
2) collection and hatching of ovum
Citrus fruit fly after Adult worms producting eggs in step 1) is adopted ovum and larva raising device to take out, to citrus fruit fly adopt ovum and
Artificial larvae feeds are added in larva raising device, then stir evenly artificial larvae feeds and mango juice, reuse spoon
Son provokes artificial larvae feeds and mango juice blend adopts the inner wall of ovum and larva raising device along citrus fruit fly, will be attached to interior
Worm's ovum on wall is viscous lower and admixes in artificial larvae feeds and mango juice blend, and citrus fruit fly is then adopted ovum and larva raising
Device is put into growth cabinet, and growth cabinet is set as 27~29 DEG C of temperature, humidity 65%~75%, photoperiod 16L：
8D；
3) larva raising
The citrus fruit fly larvae development of the egg hatching in ovum and larva raising device is adopted to aging to step 2) citrus fruit fly
When larva, citrus fruit fly is adopted into ovum and larva raising device takes out from growth cabinet, opens the lid and is put into equipped with fine sand
In container, citrus fruit fly mature larva is spontaneous to be jumped out to pierce in fine sand and pupates.
Preferably, it includes box body that the citrus fruit fly, which adopts ovum and larva raising device, and the upper end of the box body is connected with box
Lid, four side walls of the box body are uniformly provided with tocostome respectively, between two adjacent tocostomes distance be 0.5~
1cm。
Preferably, the box body is transparent plastic box, the size of the box body be 17cm × 11.5cm ×
6.7cm, wall thickness 0.35mm.
Preferably, the aperture of the tocostome is 0.6mm, the production of lower side on four side walls of the box body
The bottom surface of micropyle and box body distance is at least 1.5cm.
Preferably, the artificial larvae feeds raw material composition and corresponding parts by weight are：Bean dregs 45g, corn flour 4g, Huang
Bean powder 3g, sucrose 14g, yeast powder 7g, methyl p-hydroxybenzoate 0.3g, 3~5ml of acetic acid, 20~25ml of distilled water.
Compared with prior art, the beneficial effects of the invention are as follows：
1, a kind of citrus fruit fly of the present invention adopts the method and device of ovum and larva raising, is biology, the life of citrus fruit fly
The further investigation offer quantity in Neo-Confucianism, behaviouristics, science of heredity, ecology, especially Prevention Technique field is big, and development is neat, raw
The good worm sources material of reason situation consistency, and the citrus fruit fly being related to adopts ovum and larva raising apparatus structure is simple, recyclable
Using, it is low in cost, occupy little space, facilitate biological control, saving manpower easy to operate.
2, a kind of citrus fruit fly of the present invention adopts the method and device of ovum and larva raising, citrus fruit fly oviposition is lured, ovum
Collection be brought together with hatching together with larva raising, realize and adopt the seamless connection of ovum to larva raising：In the same tangerine
Small trypetid, which is adopted, to be added after mango juice lures citrus fruit fly Adult worms producting eggs in ovum and larva raising device, is directly added artificial larva and is raised
Ovum around tocostome, is sticked on feed using artificial larvae feeds and mango juice blend, admixes in feed and incubate to it by material
Change, in contrast to worm's ovum is obtained by washing and filtering in the prior art, then worm's ovum is transferred in the device equipped with larvae feeds
Larva raising is carried out, the method for the present invention can collect all ovum grains completely, effectively improve worm's ovum yield, while reduce other operations
To the mechanical wounding of ovum, guarantees that citrus fruit fly development is neat, physiological status consistency is good, effectively improve the hatching rate of ovum, reach
To prolific purpose.
3, a kind of citrus fruit fly of the present invention adopts the method and device of ovum and larva raising, is made using rectangle transparent plastic box
Ovum and larva raising device are adopted for citrus fruit fly, is developed to the mature larva stage in newly hatched larvae, box cover is conducive to artificial larva
Feed moisturizing, tocostome are conducive to limited air circulation, and box body is transparent to be convenient for breeding observing, and box body is square can be tight up and down
Solid matter column, save the usable floor area of growth cabinet, are convenient for biological control, open the lid, citrus fruit fly is adopted ovum and larva
Breeding apparatus be put into equipped be suitable for humidity fine sand container in, at this time citrus fruit fly mature larva it is spontaneous jump out to pierce in fine sand change
Pupa, it is easy to operate, save manpower.
4, a kind of citrus fruit fly of the present invention adopts the method and device of ovum and larva raising, adopts ovum and larva using citrus fruit fly
Citrus fruit fly is carried out that oviposition is lured, the collection of ovum and hatching are brought together together with larva raising, citrus fruit fly by breeding apparatus
In clamped condition when adopting box body and long box cover in ovum and larva raising device, therefore it is small to have evaded common laboratory rearing tangerine
Trypetid, which passes through, places the tropical fruit (tree)s such as banana in insect cage, ovum is produced and bred in fruit by female adult, and fruit rots and gives out difficulty
The phenomenon that smelling and a large amount of drosophila of generation, pollution laboratory environment.
Detailed description of the invention
Fig. 1 is that citrus fruit fly of the invention adopts ovum and larva raising apparatus structure schematic diagram.
In figure：1- box body, 2- box cover, 3- tocostome.
Specific embodiment
Below in conjunction with the embodiment of the present invention, technical solution of the present invention is clearly and completely described, it is clear that institute
The embodiment of description is only a part of the embodiment of the present invention, instead of all the embodiments.Based on the embodiments of the present invention,
Every other embodiment obtained by those of ordinary skill in the art without making creative efforts, belongs to this hair
The range of bright protection.
Embodiment 1：
Referring to Fig. 1, producing citrus fruit fly first adopts ovum and larva raising device, box body uses transparent plastic box, box
The size of body 1 is 17cm × 11.5cm × 6.7cm, and wall thickness 0.35mm, the upper end of box body is connected with box cover 2, four sides of box body
Wall uniformly opens tocostome 3 respectively, and tocostome aperture is 0.6mm, and distance is 0.5cm between two adjacent tocostomes, is located at
The bottom surface distance of the tocostome and box body of lower side is 1.5cm on four side walls of box body.
Then it carries out citrus fruit fly and adopts ovum and larva raising
1) oviposition is lured
Mango juice is added to citrus fruit fly to adopt in ovum and larva raising device, mango juice adopts ovum and larva in citrus fruit fly
It is 1cm that depth is added in breeding apparatus, after addition, covers tightly box cover, citrus fruit fly is adopted ovum and larva raising device is put into
In the rearing-box of the citrus fruit fly in egg-laying season, for citrus fruit fly Adult worms producting eggs, ovum is adopted to citrus fruit fly daily and larva raising fills
It is primary to set replacement；
Randomly select 4 citrus fruit flies in step 1) and adopt ovum and larva raising device, each citrus fruit fly adopt ovum and
The length and width face of larva raising device is each to select 5 tocostome statistics ovum grain number amounts at random, is shown in Table 1
1 citrus fruit fly of table adopts ovum and larva raising device acquisition ovum grain number amount
Artificial larvae feeds production：
By bean dregs 45g, corn flour 4g, soybean powder 3g, sucrose 14g, yeast powder 7g, methyl p-hydroxybenzoate 0.3g, vinegar
Sour 3ml is added in 20ml distilled water and is uniformly mixed, and mixture is carried out microwave heating using preceding, heating time 1min adds
It is taken out after heat, cooled to room temperature can be used.
2) collection and hatching of ovum
Citrus fruit fly after Adult worms producting eggs in step 1) is adopted ovum and larva raising device to take out, to citrus fruit fly adopt ovum and
Artificial larvae feeds are added in larva raising device, then stir evenly artificial larvae feeds and mango juice, reuse spoon
Son provokes artificial larvae feeds and mango juice blend adopts the inner wall of ovum and larva raising device along citrus fruit fly, will be attached to interior
Worm's ovum on wall is viscous lower and admixes in artificial larvae feeds and mango juice blend, and citrus fruit fly is then adopted ovum and larva raising
Device is put into growth cabinet, and growth cabinet is set as 27 DEG C of temperature, humidity 65%, photoperiod 16L：8D；
3) larva raising
The citrus fruit fly larvae development of the egg hatching in ovum and larva raising device is adopted to aging to step 2) citrus fruit fly
When larva, citrus fruit fly is adopted into ovum and larva raising device takes out from growth cabinet, opens the lid and is put into environment temperature 25
DEG C, in the container equipped with fine sand of humidity 70%, citrus fruit fly mature larva is spontaneous to be jumped out to pierce in fine sand and pupates.
4 citrus fruit flies are randomly selected in step 2) and adopt ovum and larva raising device, count the hatching of bactrocera dorsalis ovum
Rate and larvae pupation rate, are shown in Table 2
Wherein：Hatching rate=hatch larva number/test ovum number × 100%
Larva number × 100% that percentage of pupation=number/egg hatching of pupating goes out
The hatching rate and larvae pupation rate of 2 bactrocera dorsalis ovum of table
Embodiment 2：
Referring to Fig. 1, producing citrus fruit fly first adopts ovum and larva raising device, box body uses transparent plastic box, box
The size of body 1 is 17cm × 11.5cm × 6.7cm, and wall thickness 0.35mm, the upper end of box body is connected with box cover 2, four sides of box body
Wall uniformly opens tocostome 3 respectively, and tocostome aperture is 0.6mm, and distance is 0.8cm between two adjacent tocostomes, is located at
The bottom surface distance of the tocostome and box body of lower side is 1.8cm on four side walls of box body.
Then it carries out citrus fruit fly and adopts ovum and larva raising
1) oviposition is lured
Mango juice is added to citrus fruit fly to adopt in ovum and larva raising device, mango juice adopts ovum and larva in citrus fruit fly
It is 1.2cm that depth is added in breeding apparatus, after addition, covers tightly box cover, citrus fruit fly is adopted ovum and larva raising device is put
In the rearing-box for entering the citrus fruit fly in egg-laying season, for citrus fruit fly Adult worms producting eggs, ovum and larva raising are adopted to citrus fruit fly daily
Device replacement is primary；
Randomly select 4 citrus fruit flies in step 1) and adopt ovum and larva raising device, each citrus fruit fly adopt ovum and
The length and width face of larva raising device is each to select 5 tocostome statistics ovum grain number amounts at random, is shown in Table 1
1 citrus fruit fly of table adopts ovum and larva raising device acquisition ovum grain number amount
Artificial larvae feeds production：
By bean dregs 45g, corn flour 4g, soybean powder 3g, sucrose 14g, yeast powder 7g, methyl p-hydroxybenzoate 0.3g, vinegar
Sour 4ml is added in 22ml distilled water and is uniformly mixed, and mixture is carried out microwave heating using preceding, heating time 1.5min,
It is taken out after heating, cooled to room temperature can be used.
2) collection and hatching of ovum
Citrus fruit fly after Adult worms producting eggs in step 1) is adopted ovum and larva raising device to take out, to citrus fruit fly adopt ovum and
Artificial larvae feeds are added in larva raising device, then stir evenly artificial larvae feeds and mango juice, reuse spoon
Son provokes artificial larvae feeds and mango juice blend adopts the inner wall of ovum and larva raising device along citrus fruit fly, will be attached to interior
Worm's ovum on wall is viscous lower and admixes in artificial larvae feeds and mango juice blend, and citrus fruit fly is then adopted ovum and larva raising
Device is put into growth cabinet, and growth cabinet is set as 28 DEG C of temperature, humidity 70%, photoperiod 16L：8D；
3) larva raising
The citrus fruit fly larvae development of the egg hatching in ovum and larva raising device is adopted to aging to step 2) citrus fruit fly
When larva, citrus fruit fly is adopted into ovum and larva raising device takes out from growth cabinet, opens the lid and is put into environment temperature 25
DEG C, in the container equipped with fine sand of humidity 70%, citrus fruit fly mature larva is spontaneous to be jumped out to pierce in fine sand and pupates.
4 citrus fruit flies are randomly selected in step 2) and adopt ovum and larva raising device, count the hatching of bactrocera dorsalis ovum
Rate and larvae pupation rate, are shown in Table 2
Wherein：Hatching rate=hatch larva number/test ovum number × 100%
Larva number × 100% that percentage of pupation=number/egg hatching of pupating goes out
The hatching rate and larvae pupation rate of 2 bactrocera dorsalis ovum of table
Embodiment 3：
Referring to Fig. 1, producing citrus fruit fly first adopts ovum and larva raising device, box body uses transparent plastic box, box
The size of body 1 is 17cm × 11.5cm × 6.7cm, and wall thickness 0.35mm, the upper end of box body is connected with box cover 2, four sides of box body
Wall uniformly opens tocostome 3 respectively, and tocostome aperture is 0.6mm, and distance is 1cm between two adjacent tocostomes, is located at box
The bottom surface distance of the tocostome and box body of lower side is 2cm on four side walls of body.
Then it carries out citrus fruit fly and adopts ovum and larva raising
1) oviposition is lured
Mango juice is added to citrus fruit fly to adopt in ovum and larva raising device, mango juice adopts ovum and larva in citrus fruit fly
It is 1.5cm that depth is added in breeding apparatus, after addition, covers tightly box cover, citrus fruit fly is adopted ovum and larva raising device is put
In the rearing-box for entering the citrus fruit fly in egg-laying season, for citrus fruit fly Adult worms producting eggs, ovum and larva raising are adopted to citrus fruit fly daily
Device replacement is primary；
Randomly select 4 citrus fruit flies in step 1) and adopt ovum and larva raising device, each citrus fruit fly adopt ovum and
The length and width face of larva raising device is each to select 5 tocostome statistics ovum grain number amounts at random, is shown in Table 1
1 citrus fruit fly of table adopts ovum and larva raising device acquisition ovum grain number amount
Artificial larvae feeds production：
By bean dregs 45g, corn flour 4g, soybean powder 3g, sucrose 14g, yeast powder 7g, methyl p-hydroxybenzoate 0.3g, vinegar
Sour 5ml is added in 25ml distilled water and is uniformly mixed, and mixture is carried out microwave heating using preceding, heating time 2min adds
It is taken out after heat, cooled to room temperature can be used.
2) collection and hatching of ovum
Citrus fruit fly after Adult worms producting eggs in step 1) is adopted ovum and larva raising device to take out, to citrus fruit fly adopt ovum and
Artificial larvae feeds are added in larva raising device, then stir evenly artificial larvae feeds and mango juice, reuse spoon
Son provokes artificial larvae feeds and mango juice blend adopts the inner wall of ovum and larva raising device along citrus fruit fly, will be attached to interior
Worm's ovum on wall is viscous lower and admixes in artificial larvae feeds and mango juice blend, and citrus fruit fly is then adopted ovum and larva raising
Device is put into growth cabinet, and growth cabinet is set as 29 DEG C of temperature, humidity 75%, photoperiod 16L：8D；
3) larva raising
The citrus fruit fly larvae development of the egg hatching in ovum and larva raising device is adopted to aging to step 2) citrus fruit fly
When larva, citrus fruit fly is adopted into ovum and larva raising device takes out from growth cabinet, opens the lid and is put into environment temperature 25
DEG C, in the container equipped with fine sand of humidity 70%, citrus fruit fly mature larva is spontaneous to be jumped out to pierce in fine sand and pupates.
4 citrus fruit flies are randomly selected in step 2) and adopt ovum and larva raising device, count the hatching of bactrocera dorsalis ovum
Rate and larvae pupation rate, are shown in Table 2
Wherein：Hatching rate=hatch larva number/test ovum number × 100%
Larva number × 100% that percentage of pupation=number/egg hatching of pupating goes out
The hatching rate and larvae pupation rate of 2 bactrocera dorsalis ovum of table
A kind of citrus fruit fly of the present invention adopts the method and device of ovum and larva raising, in contrast to the prior art, unit area
Citrus fruit fly raises out that quantity is big, while the hatching rate of citrus fruit fly, percentage of pupation be higher and development is neat, physiological status consistency
Well.
It although an embodiment of the present invention has been shown and described, for the ordinary skill in the art, can be with
A variety of variations, modification, replacement can be carried out to these embodiments without departing from the principles and spirit of the present invention by understanding
And modification, the scope of the present invention is defined by the appended.
Claims (5)
1. the method and device that a kind of citrus fruit fly adopts ovum and larva raising, it is characterised in that：Its method includes the following steps：
1) oviposition is lured
Mango juice is added to citrus fruit fly to adopt in ovum and larva raising device, mango juice adopts ovum and larva raising in citrus fruit fly
It is 1~1.5cm that depth is added in device, after addition, covers tightly box cover, citrus fruit fly is adopted ovum and larva raising device is put into
In the rearing-box of the citrus fruit fly in egg-laying season, for citrus fruit fly Adult worms producting eggs, ovum is adopted to citrus fruit fly daily and larva raising fills
It is primary to set replacement；
2) collection and hatching of ovum
Citrus fruit fly after Adult worms producting eggs in step 1) is adopted into ovum and larva raising device takes out, adopts ovum and larva to citrus fruit fly
Artificial larvae feeds are added in breeding apparatus, then stir evenly artificial larvae feeds and mango juice, reuses spoon and chooses
It plays artificial larvae feeds and mango juice blend adopts the inner wall of ovum and larva raising device along citrus fruit fly, will be attached on inner wall
Worm's ovum it is viscous lower and admix in artificial larvae feeds and mango juice blend, citrus fruit fly is then adopted into ovum and larva raising device
It is put into growth cabinet, growth cabinet is set as 27~29 DEG C of temperature, humidity 65%~75%, photoperiod 16L：8D；
3) larva raising
The citrus fruit fly larvae development of the egg hatching in ovum and larva raising device is adopted to mature larva to step 2) citrus fruit fly
When, citrus fruit fly is adopted into ovum and larva raising device takes out from growth cabinet, opens the lid and is put into the container equipped with fine sand
In, citrus fruit fly mature larva is spontaneous to be jumped out to pierce in fine sand and pupates.
2. the method and device that a kind of citrus fruit fly according to claim 1 adopts ovum and larva raising, it is characterised in that：Institute
State that citrus fruit fly adopts ovum and larva raising device includes box body (1), the upper end of the box body (1) is connected with box cover (2), the box
Four side walls of body (1) are uniformly provided with tocostome (3) respectively, between the tocostome (3) of adjacent two distance be 0.5~
1cm。
3. the method and device that a kind of citrus fruit fly according to claim 2 adopts ovum and larva raising, it is characterised in that：Institute
Stating box body (1) is transparent plastic box, and the size of the box body (1) is 17cm × 11.5cm × 6.7cm, wall thickness 0.35mm.
4. the method and device that a kind of citrus fruit fly according to claim 2 adopts ovum and larva raising, it is characterised in that：Institute
The aperture for stating tocostome (3) is 0.6mm, is located at the tocostome (3) of lower side and institute on four side walls of the box body (1)
The bottom surface distance for stating box body (1) is at least 1.5cm.
5. the method and device that a kind of citrus fruit fly according to claim 1 adopts ovum and larva raising, it is characterised in that：Institute
It states artificial larvae feeds raw material composition and corresponding parts by weight is：Bean dregs 45g, corn flour 4g, soybean powder 3g, sucrose 14g, ferment
Female powder 7g, methyl p-hydroxybenzoate 0.3g, 3~5ml of acetic acid, 20~25ml of distilled water.
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN115067281A (en) * | 2022-06-13 | 2022-09-20 | 云南省林业和草原科学院 | Method for interfering egg laying behavior of citrus fruit fly adults by using sound |
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| CN202907656U (en) * | 2012-10-16 | 2013-05-01 | 西南大学 | Fruit fly class injurious insect single head feeding and insect egg collecting device |
| CN107926869A (en) * | 2017-12-27 | 2018-04-20 | 贵州大学 | A kind of imago breeding equipment of Tephritidae insect |
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| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN202907656U (en) * | 2012-10-16 | 2013-05-01 | 西南大学 | Fruit fly class injurious insect single head feeding and insect egg collecting device |
| CN107926869A (en) * | 2017-12-27 | 2018-04-20 | 贵州大学 | A kind of imago breeding equipment of Tephritidae insect |
Non-Patent Citations (1)
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN115067281A (en) * | 2022-06-13 | 2022-09-20 | 云南省林业和草原科学院 | Method for interfering egg laying behavior of citrus fruit fly adults by using sound |
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