CN202907656U

CN202907656U - Fruit fly class injurious insect single head feeding and insect egg collecting device

Info

Publication number: CN202907656U
Application number: CN 201220529146
Authority: CN
Inventors: 王进军; 魏冬; 豆威; 魏丹丹; 夹福先
Original assignee: Southwest University
Current assignee: Southwest University
Priority date: 2012-10-16
Filing date: 2012-10-16
Publication date: 2013-05-01
Anticipated expiration: 2022-10-16

Abstract

A fruit fly class injurious insect single head feeding and insect egg collecting device is in a cylinder shape, and composed of an inner cylinder 1, an outer cylinder 3, a trapping collector 5 and a feed box 6, wherein the outer cylinder 3 is slightly larger than the inner cylinder 1 and tightly fits with the inner cylinder 1, the top 2 of the inner cylinder 1 and the bottom 7 of the outer cylinder 3 are adhesively sealed by using gauze with the mesh not larger than 0.1mm, a cross section on the upper portion of the outer cylinder 3 is an inclined plane, the side wall of the inner cylinder 1 is provided with an open mouth 4, the height of the open mouth 4 is between the upper and the lower heights of the inclined cross section of the outer cylinder 3, and the trapping collector 5 is a centrifuge tube with an open hole at a sealed mouth end. The fruit fly class injurious insect single head feeding and insect egg collecting device is simple and convenient to manufacture, low in cost, convenient to operate and good in air permeability, can achieve single head mass feed of small insects and fly insects, and furthermore obtained biology information is accurate and reliable.

Description

A kind of Fruit fly single head is raised and the worm's ovum gathering unit

Technical field

The utility model relates to the plant protection technology field, is specifically related to a kind of Fruit fly single head and raises and the worm's ovum gathering unit.

Background technology

Fruit fly belongs to diptera Diptera, Tephritidae Tephritidae, of a great variety, be distributed widely in all over the world serious harm Perenniporia martius economic crops, especially fruits and vegetables can be caused huge economic loss, is the important pest of a class.The in recent years unreasonable use of chemical pesticide causes the pesticide resistance of Fruit fly to increase sharply, and its prevention and control difficulty strengthens; In addition, along with China joined WTO and opening further enlarge, fruits and vegetables industry trade both at home and abroad is day by day frequent, and the propagation risk of all kinds of pests also further increases.Along with the impact of the factors such as Global climate change, the suitable life of Fruit fly and distribution are all enlarging (such as citrus fruit fly, citrus fruit fly etc.) gradually.Thereby, be necessary Fruit fly is carried out deep basic research, for its control proposes Counter-measures.Wherein, the fundamental biological knowledge research of Fruit fly is the prerequisite of carrying out other correlative studys.China starts late for the research of Tephritidae insect, and its biological research is relatively lagged behind especially.In the biological study of insect, usually need single head raising or pair fed etc., and Fruit fly because it has the ability of flight, has brought very large trouble to correlative study work, it then is more difficult that single head is raised.

Traditional large-scale breeding cage is still adopted in the at present raising of Fruit fly, and breeding cage generally adopts the aluminium frame to do support, and the four sides adopts wire netting to do wall to increase gas permeability.This device not only takes up room, and cost is high, and experimental data is repeated relatively poor.

In addition, in the biology of reproduction of Fruit fly and physiology of reproduction research process, also relate to the statistics of insect spawning amount and incubation rate.Traditional method is to put into single head or paired adult in large breeding cage, adds the fruit such as fruit as laying eggs medium, at last ovum grain quantity in each oviporous orifice is added up in each oviporous orifice rip cutting, as the fecundity parameter of female worm.In traditional statistical, ovum quantitative statistics method error is added up in the artificial incision of oviporous orifice more very large, and also the little paler colour of ovum, close with the color of fruit pulp, be difficult for observing.In addition, the quantity of each oviporous orifice is also inconsistent, and these all can impact result of study.And insect fertility not only is embodied on the egg laying amount, also and the incubation rate of ovum be closely related, only have the fertility that the quantity of hatching ovum could real embodiment insect.In actual research work, when the ovum of Fruit fly was collected, often because breeding cage is too large, and the breeding cage cornice had aperture, and adult directly produces the ovum grain on the breeding cage wall, is not easy to the collection of worm's ovum, and also the people is the reduction that causes incubation rate.

Therefore be necessary to invent and a kind ofly make simple and easy, economical and the raising device of easy-operating Fruit fly, the experiment of the ecology of class pest facilitates for this reason.

Summary of the invention

The purpose of this utility model is to provide a kind of Fruit fly single head to raise and the worm's ovum gathering unit.This device can realize that small insects and flying insect single head raise in enormous quantities, and is convenient to the collection of worm's ovum, guarantees the higher incubation rate of ovum.

A kind of Fruit fly single head described in the utility model is raised and the worm's ovum gathering unit, profile is cylinder, by inner core 1., urceolus 3., 6. 5. trapper form with feed box, 1. and with it 3. urceolus is slightly larger than inner core closely agrees with, 7. 2. inner core top 1. be not more than the gauze seal of 0.1 mm with mesh with urceolus bottom 3., 3. urceolus goes up cross section is the inclined-plane, 4. inner core 1. sidewall is provided with an opening, opening height 4. is between urceolus 3. between the upper and lower height in transversal inclined-plane, and 5. trapper is the centrifuge tube of sealing one end perforate.

Inner core 1., urceolus 3. available transparent rigid plastics make, 4. opening is used for the examination worm, 5. trapper reaches feed box turnover 6..3. 1. inner core can arbitrarily rotate with urceolus, and 4. opening is opened and closed freely.When needs were changed feed or other operation, 1. the rotation inner core made opening 4. be in open mode and gets final product, and has operated timely rotary closing opening 4., and 2. inner core top 1. can guarantee to breathe freely with urceolus bottom 3. gauze 7..

During use, 1. the rotation inner core makes opening 4. forward 3. transversal inclined-plane, top downside of urceolus to, and preprepared examination worm is transferred in this device, and add adult forage to feed box 6., the rotation inner core 1. make opening 4. forward urceolus to 3. the high side closure opening in transversal inclined-plane, top is 4.; This device is placed suitable temp. and humidity environment breeding observing.After luring collection complete, take out trapper 5., obtain testing required worm's ovum on the moistening gauze for test (RNA extracts for egg laying amount statistics, incubation rate statistics, DNA extraction, various enzyme activity determinations etc.) with luring liquid collecting to be poured on.

Luring in the collection process of Fruit fly raising or ovum, the introducing of each examination worm, feed box are 6., 4. trapper turnover 5. all pass in and out from opening, and feed box is diameter 15 mm of available upper end open 6., the cylindrical shape etui of high 10 mm more is conducive to change feed in feeding process.

The utility model has the advantages that:

(1) simple for production, easy to operate, good permeability can realize that small insects and flying insect single head raise in enormous quantities.

(2) raise insect with this device, not only with low cost, and conserve space.Compare with large-scale traditional breeding cage, can avoid resource (electric power energy, artificial feed etc.) waste.

(3) with this device Fruit fly is carried out single head and raise, can make the various conditions of examination worm raising reach homogeneous and accurate, guarantee the feeding environment of relative ideal, the biological information of acquisition more accurately, reliably.

(4) incubation rate of ovum is very high, and the RNA that ovum extracts and the activity of DNA are also higher, can satisfy the requirement of follow-up related science test fully.

Description of drawings

Fig. 1 is that a kind of Fruit fly single head of the utility model is raised and worm's ovum gathering unit structural representation.

Embodiment

The implementation step that following basis is concrete is described in further detail the utility model by reference to the accompanying drawings.

(1) rigid plastics paper is cut into the rectangle of 100 mm * 225 mm, curling bonding approximately 5 mm make diameter 70 mm, the inner core of high 100 mm 1., an inner core 1. end is that 2. the gauze seal of 0.1 mm becomes the top with the hole order, inner core 1. 20 mm places, lower end open 15 mm * 12 mm opening 4., the turnover of convenient examination worm and the replacing of feed, as shown in the figure.

(2) to make diameter be 72 mm for same procedure and material, the urceolus of high 70 mm 3., making one end cross section is the inclined-plane, the height of lowest part is 15 mm, other end seal hole order is that 7. the gauze of 0.1 mm makes the bottom, just in time entangle inner core 1., in put feed box and 6. namely make raising device, as shown in the figure.

The collection of Fruit fly ovum is based on above-mentioned raising device, and the trapper of the ovum of also need to packing in raising device 5..5. trapper adopts the centrifuge tube of 2 mL as luring the collection carrier, the close sealing of centrifuge tube one end approximately 10 mm place tube wall four directions is evenly opened respectively four row's apertures (as shown in the figure), makes things convenient for trypetid to lay eggs, pitch-row 5 mm, every pipe amounts to 16 holes, and centrifuge tube lid one end then is transparent imperforate section.Concrete steps are as follows:

(1) will lure liquid collecting or other to have to lure the material (orange juice etc.) of collection effect pack into trapper 5. in, the liquid level of liquid reaches 3/4 of pipe height and gets final product, the peak of solid bait also need reach 3/4 place that lures collector, cover lid;

(2) with ready trapper 5. lid be stained with pressure sensitive adhesive double coated for subsequent use;

(3) before the egg production peak time of examination worm, 5. ready trapper is put into the raising device of examination worm with tweezers, utilize double faced adhesive tape that 5. trapper is fixed on the bottom.

(4) adult forage is added to feed box 6. in, this device is placed under the suitable temperature and humidity conditions raise again.

(5) wait to lure collection complete, take out trapper 5. with tweezers, obtain testing required worm's ovum on the moistening gauze for counting or follow-up research with luring liquid collecting to be poured on.

Take citrus fruit fly as example, utilize this device to observe Life of Adult and the fecundity life table of citrus fruit fly.Ready examination worm is transferred to pair fed in this device (the male and female proportioning under the natural conditions is 1:1), put into artificial feed and take orange juice as the trapper that lures liquid collecting 5., in climatic cabinate, raise (temperature: 27.5 ± 0.5 ℃, humidity: 75 ± 0.5%, the photoperiod: L:D=14 h:10 h).5. trapper puts into this device at 14:00, in about 21:00, take out (egg production peak time of citrus fruit fly mainly concentrates at dusk front and back), filter with gauze and ovum to be counted and to be transferred on the artificial feed after luring liquid collecting, just can count after 5 days, with the incubation rate of statistics ovum.Can obtain more accurately test data with this device, (climatic cabinate can only be placed 2 of traditional breeding cages, and apparatus of the present invention then can be placed 100 at least) can also economize on resources.Collect the ovum of Fruit fly by the method, not only data accurately, reliably can also guarantee the activity of ovum, are convenient to follow-up research.

Claims

1. a Fruit fly single head is raised and the worm's ovum gathering unit, profile is cylinder, it is characterized in that by inner core 1., urceolus 3., 6. 5. trapper form with feed box, 1. and with it 3. urceolus is slightly larger than inner core closely agrees with, 7. 2. inner core top 1. be not more than the gauze seal of 0.1 mm with mesh with urceolus bottom 3., 3. urceolus goes up cross section is the inclined-plane, 4. inner core 1. sidewall is provided with an opening, opening height 4. is between urceolus 3. between the upper and lower height in transversal inclined-plane, and 5. trapper is the centrifuge tube of sealing one end perforate.

2. a kind of Fruit fly single head according to claim 1 is raised and the worm's ovum gathering unit, it is characterized in that inner core 1., 3. urceolus make with transparent rigid plastics.