CN114557320A - Device for raising adult fruit fly insects and measuring biological parameters and using method - Google Patents
Device for raising adult fruit fly insects and measuring biological parameters and using method Download PDFInfo
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- CN114557320A CN114557320A CN202210196935.6A CN202210196935A CN114557320A CN 114557320 A CN114557320 A CN 114557320A CN 202210196935 A CN202210196935 A CN 202210196935A CN 114557320 A CN114557320 A CN 114557320A
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Images
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K67/00—Rearing or breeding animals, not otherwise provided for; New or modified breeds of animals
- A01K67/033—Rearing or breeding invertebrates; New breeds of invertebrates
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A50/00—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE in human health protection, e.g. against extreme weather
- Y02A50/30—Against vector-borne diseases, e.g. mosquito-borne, fly-borne, tick-borne or waterborne diseases whose impact is exacerbated by climate change
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- Life Sciences & Earth Sciences (AREA)
- Environmental Sciences (AREA)
- Animal Behavior & Ethology (AREA)
- Zoology (AREA)
- Animal Husbandry (AREA)
- Biodiversity & Conservation Biology (AREA)
- Catching Or Destruction (AREA)
Abstract
The invention relates to a device for raising adult fruit fly insects and measuring biological parameters and a using method thereof, wherein the device comprises an inner box, an outer box, a feeding tray, an egg taking device and a fixed tray; the inner box comprises an inner box net cover, an inner box ventilation hole, an inner box body and an inner box operation hole, and the inner box ventilation hole is opposite to the inner box operation hole; the outer box comprises an outer box body, an outer box ventilation hole, an outer box operation hole and a base, wherein the outer box ventilation hole is opposite to the outer box operation hole in position, and the outer box body is tightly bonded with the base; the egg taking device does not see the bottle body and the cover with the egg taking hole; the fixed tray comprises four object placing holes, five magnet positioning holes and matched fixed iron sheets and magnets. According to the device, the relative position of the operation holes of the inner box and the outer box can be adjusted by rotating the outer box through sleeving the inner box and the outer box, so that the operation in daily feeding can be conveniently carried out, the problem that test insects escape in the biological parameter measurement of the adult fruit flies is solved, and the feed is prevented from falling off in the transfer of the device.
Description
Technical Field
The invention belongs to the technical field of insect feeding, and particularly relates to a device for feeding fruit fly insect adults and measuring biological parameters and a using method thereof.
Background
The fruit fly insects are Diptera, insects of tephritidae, and most of them are world-wide important agricultural pests (Qi Xie and Zhangjie, 2005). Taking bactrocera dorsalis as an example, the bactrocera dorsalis is native to tropical and subtropical regions of asia, is distributed in provinces and cities of Yunnan, Sichuan, Guizhou, Guangdong and the like in China, has a wide host range, and can damage over 46 families of over 250 plants (Reyard et al, 2021) such as mangoes, bananas, apples and the like. The insect states are divided into eggs, larvae, pupae and imagoes. The female adult punctures the fruit epidermis with the egg laying device, lays down a plurality of eggs under the epidermis, and the mechanical damage that the egg laying hole caused provides the advantage for the invasion of other germ. After the eggs hatch, the larvae further eat to damage the fruits, resulting in the fruits rotting and falling off (guotonga et al, 2019). The larvae bore to damage the fruits, and do not climb out of the fruits until the mature larvae jump out of the fruits, and the larvae jump to find a suitable place for overwintering, and pupate in wet sand with the depth of 8cm at the deepest in the field of laboratories (Xiaoshu et al, 2001). The female adult lays 40 eggs or more in daily, the single female egg laying amount can reach 1800 eggs (Choi et al., 2020), the flying capacity is strong, and the female adult can fly for 46.5km at most (Louisa et al., 2020). The citrus fruit fly has the characteristics of wide host range, high reproductive capacity, strong flight capacity and the like, so that the citrus fruit fly is difficult to control in the field.
In the current comprehensive control of the fruit flies, chemical control is an efficient and rapid method and is also a main control method of the current fruit flies. Chemical control is mainly divided into spraying insecticide and bait trapping, wherein organophosphorus insecticides, pyrethroid insecticides, carbamate insecticides and the like are proved to be effective in controlling bactrocera dorsalis (Jinmengjiao et al, 2021; liatal, 2021). Chemical agents are widely used to manage fruit flies, and the long-term use of a single type of insecticide is liable to cause resistance. The Octopus yunnanensis et al monitors the drug resistance change of bactrocera dorsalis, and finds that the drug resistance of bactrocera dorsalis adults to abamectin and trichlorfon is obviously increased. Insects have different sensitivities to different insecticides, and insects of different insect states at different ages in days have different sensitivities to the same insecticide. Lu Yan Hui et al (2019) studied the poisoning effect of different insecticides on Spodoptera frugiperda larvae of different instars, and the results showed that 4.5% lambda-cyhalothrin emulsifiable concentrate and 1.8% abamectin microemulsion have reduced toxicity to 3 instars compared with those in the case of the first hatched larvae. Huang Yu et al (2020) determined the toxicity of four insecticides against south Asia fruit fly at different ages in days by using the stomach toxicity method, and found that the toxicity of each agent decreases with increasing age in days. The citrus fruit fly has strong adaptability to the environment, and the distribution range of the citrus fruit fly is gradually enlarged. The phyllostachys (2009) divides the distribution of bactrocera dorsalis in Yunnan into 3 areas, the area with north of sikuai, yao and qujing is the non-distribution area of the bactrocera dorsalis, and qujing is the most eastern part of seasonal distribution. The Zhaotong region in northeast Yunnan is located in the north of Qujing and is the original region of Bactrocera dorsalis. And the original non-distribution area is distributed in a large quantity nowadays.
The fruit fly insects have strong production damage and environmental adaptability, and the detection of the biological characteristics of the field fruit fly insects has important significance for guiding production. Indoor raising of fruit fly is not possible, but transparent glass raising pots are often used for raising fruit fly adults. The inconvenience of this feeding method is: the glass rearing tanks are heavy in weight and difficult to move in handling. And the glass rearing tank is usually provided with an opening only above the rearing tank, so that the test fruit flies can gather at the operation opening due to the tendency of the insects in the lower rearing process, which can cause the escape of the test insects in the operation. In addition, the traditional glass rearing pots are easy to damage and can not be overlapped, and the glass rearing pots easily occupy larger space when in use and have limited utilization on the space of the artificial climate box.
Disclosure of Invention
The invention aims to solve the problem and defect of the glass feeding tank and provides the device which is simple in structure, light in weight, easy to store and convenient for feeding the fruit fly insect adults and measuring biological parameters. The device is convenient to use, avoids the problem that living insects escape from the glass feeding tank when the glass feeding tank is used, and enables the daily observation operation in the biological parameter measurement to be quick and simple; meanwhile, the built-in fixed tray reduces the movement of the water supply cotton balls and the feed in the operation, prevents the cotton balls caused by the operation from moving and pressing the fruit flies, and reduces the workload caused by the extra repetition number required in the experiment.
The invention is realized by adopting the following technical scheme.
The device for raising the adult fruit fly insects and measuring biological parameters comprises an inner box, an outer box, a feeding tray, an egg taking device and a fixed tray;
the inner box is placed in the outer box, and the fixed tray is placed in the outer box;
the inner box comprises an inner box mesh cover, an inner box ventilation hole, an inner box body and an inner box operation hole, wherein the inner box ventilation hole and the inner box operation hole are oppositely arranged on the side wall of the inner box body;
the outer box comprises an outer box body, an outer box ventilation hole, an outer box operation hole and a base, wherein the outer box ventilation hole and the outer box operation hole are oppositely arranged on the side wall of the outer box body, and the outer box body is tightly bonded with the base;
the ventilation holes of the inner box and the outer box are provided with ventilation nets;
the egg taking device comprises a bottle body and a cover, and an egg taking hole is formed in the bottle body; the bottle body is connected with the cover through threads;
the fixed tray comprises a storage hole;
a plurality of magnet positioning holes are formed in the fixing tray, and magnets are arranged in the magnet positioning holes;
the bottom of the box body is provided with a fixed iron sheet which is positioned outside the bottom of the outer box.
The inner box net cover is made of transparent PVC, the radius of the inner box net cover is 101.50mm, a circular opening is formed in the center of the inner box net cover, the radius of the inner box net cover is 78.50mm, and a first 40-mesh gauze is laid in the circular opening.
The ventilation net comprises an arc-shaped fixed frame, and holes are formed in the arc-shaped fixed frame and the second gauze with 40 meshes is laid in the holes.
The inner box body is made of transparent PVC, the size of the inner box body is 103.00mm, the size of the inner box body is 120.00mm, the thickness of the wall and the box bottom is 3mm, the inner box body is provided with an inner box ventilation hole and an inner box operation hole which have the same diameter at a position 7mm away from the box bottom, and the size of the inner box operation hole is consistent with the outer size of the inner box ventilation hole;
the outer box body is made of transparent PVC, the size of the outer box body is 106.00mm, the size of the outer box body is 120.00mm, the thickness of the outer box body is 3mm, the outer box body is provided with an outer box ventilation hole and an outer box operation hole which are opposite to each other on the same diameter at a position 10mm away from the box bottom, and the size of the outer box operation hole is consistent with the outer size of the outer box ventilation hole;
the base material be transparent PVC, including r 106.00mm, the circular chassis that thickness is 3mm, be equipped with the ring shape spike of height 3mm on the circular chassis outer circumference.
The bottle body is transparent PET, the size is r is 9mm, h is 50mm, the bottom is 1mm thick, the wall thickness is 1mm, and the bottle top is provided with 6mm long bottle body threads; the egg collecting holes are small holes with r being 0.5mm, 4 rows are distributed from the position 15mm away from the bottom of the bottle, and 20 egg collecting holes are formed in total.
The lid material be PET, the size be r 11mm, h become 8 mm's cylindrical, from the lid top distribution 6 mm's bottle lid screw thread, closely spiral with the body.
The edible plate is made of PET, is cylindrical with the size r being 11mm, h being 30mm, and has the bottom thickness of 1mm and the wall thickness of 1 mm.
Fixed tray material be transparent PVC, the size be r 50mm, h 6mm, have 4 on four summits of the length of diagonal center and centre of a circle coincidence for the square of 50mm and put the thing hole, there are 5 magnet locating holes at four summits and diagonal intersection point department of square.
The radius of the object placing hole is 10mm, the depth of the object placing hole is 4mm, and a concentric magnet positioning hole is formed in the center of the object placing hole;
the magnet positioning holes are holes with r being 2.5mm and h being 2mm, and can be tightly connected with the magnets.
The magnet material cylindrical permanent magnet is a cylinder with the dimensions of r being 2.5mm and h being 2mm, and the cylindrical permanent magnet is attracted with the fixed iron sheet for fixing.
The fixed magnet is made of 204 series stainless steel, and the size of the fixed magnet is r equal to 50mm, and delta is 1 mm.
The method for raising the adult fruit fly insects and measuring the biological parameters by using the device for raising the adult fruit fly insects and measuring the biological parameters comprises the following steps:
taking out the fixed tray, clamping the magnet into the magnet positioning hole, plugging the wet cotton ball with water, the fruit fly feed and the wet cotton ball with honey water with the mass content of 15% into the food tray, and then putting the food tray into the object placing hole;
taking out the egg taking device, pouring mango juice for inducing spawning into the egg taking device, enabling the liquid level to be about 5mm away from the egg taking hole at the lower end, screwing a cover and placing the egg taking device in the object placing hole;
taking out the inner box, and clamping the ventilation net into the ventilation holes of the inner box;
taking out the outer box, and clamping the ventilation net into the ventilation hole of the outer box;
putting the assembled inner box into the assembled outer box, and putting the fixed tray filled with the food tray and the egg taking device into the inner box;
step six, covering the inner box net cover, placing the fixed iron sheet at the bottom of the outer box, and moving the fixed iron sheet to adjust the position of the fixed tray to the center through magnetic force;
and seventhly, rotating the outer box, adjusting the positions of the inner box operation hole and the outer box operation hole, leading in the eclosion female-male paired fruit flies in the same day after the inner box operation hole and the outer box operation hole are overlapped, rotating the outer box again to ensure that the inner box operation hole and the outer box ventilation hole are overlapped, and placing the device in an L (ratio D to 14): 10, RH 70%, T25 deg.C artificial climate box;
and step eight, observing the survival state of the female and male insects in the recording device every day, moving the fixed iron sheet to adjust the position of the fixed tray to the inner box operation hole and the outer box operation hole through magnetic force, replacing a fresh cotton ball and feed, pouring out mango juice in the egg collector, filtering the mango juice by using a 80-mesh gauze, counting the number of eggs until the adult insects die, and thus measuring various biological parameters obtained in the adult trypetid period.
Due to the adoption of the technical scheme, the invention has the advantages that: the device solves the problem and the defect of the glass feeding tank, has simple structure and light weight, is easy to store and is convenient for feeding the fruit fly insect adults and measuring biological parameters. The device is convenient to use, avoids the problem that living insects escape from the feeding tank when the glass feeding tank is used, and enables the daily observation operation in the biological parameter measurement to be quick and simple; meanwhile, the built-in fixed tray reduces the movement of the water supply cotton balls and the feed in the operation, prevents the cotton balls caused by the operation from moving and pressing the fruit flies, and reduces the workload caused by the extra repetition number required in the experiment. The invention has simple structure, convenient use and ideal use effect.
The invention will be further explained and illustrated with reference to the drawings and the detailed description.
Drawings
FIG. 1 is a schematic view of the open state structure of the present invention;
FIG. 2 is a schematic structural view of the present invention in a closed state;
FIG. 3 is a schematic structural view of an inner case device according to the present invention;
FIG. 4 is a schematic view of the structure of the net cover of the inner box of the present invention;
FIG. 5 is a schematic view of the inner box vent structure of the present invention;
FIG. 6 is a schematic view of an outer box device according to the present invention;
FIG. 7 is a schematic view of the bottom base of the outer box of the present invention;
FIG. 8 is a schematic view of the egg collector of the present invention;
FIG. 9 is a schematic view of a fixed tray structure according to the present invention;
FIG. 10 is a schematic view of a bowl of the present invention;
Detailed Description
In order to make the objects, technical solutions and advantages of the present invention more apparent, the present invention will be described in further detail with reference to the accompanying drawings and examples.
The device for raising adult fruit fly insects and measuring biological parameters comprises an inner box 1, an outer box 2, an edible tray 3, an egg taking device 4 and a fixed tray 5;
the inner box 1 is arranged in the outer box 2, and the fixed tray 5 is arranged in the outer box 2;
the inner box 1 comprises an inner box net cover 1.1, an inner box ventilation hole 1.2, an inner box body 1.3 and an inner box operation hole 1.4, wherein the inner box ventilation hole 1.2 and the inner box operation hole 1.4 are oppositely arranged on the side wall of the inner box body 1.3;
the outer box 2 comprises an outer box body 2.1, an outer box ventilation hole 2.2, an outer box operation hole 2.4 and a base 2.3, the outer box ventilation hole 2.2 and the outer box operation hole 2.4 are oppositely arranged on the side wall of the outer box body 2.1, and the outer box body 2.1 is tightly bonded with the base 2.3;
the ventilation holes 1.2 of the inner box and the ventilation holes 2.2 of the outer box are provided with ventilation nets;
the egg taking device 4 comprises a bottle body 4.2 and a cover 4.3, and an egg taking hole 4.1 is arranged on the bottle body 4.2; the bottle body 4.2 and the cover 4.3 are connected through threads;
the fixed tray 5 comprises a placing hole 5.4;
a plurality of magnet positioning holes 5.1 are arranged on the fixed tray 5, and magnets 5.3 are arranged in the magnet positioning holes 5.1;
the bottom of the box is provided with a fixed iron sheet 5.2, and the fixed iron sheet 5.2 is positioned outside the bottom of the outer box 2.
The inner box mesh cover 1.1 is made of transparent PVC, the radius of the inner box mesh cover is 101.50mm, a circular opening is formed in the center of the inner box mesh cover, the radius of the inner box mesh cover is 78.50mm, and a first 40-mesh gauze 1.5 is laid in the circular opening. The fruit fly insect body is more than 6mm long and more than 3mm wide, and the gauze of 40 meshes ensures the air circulation requirement in feeding and can also prevent the escape of the fruit fly.
The ventilation net comprises an arc-shaped fixed frame 1.7, and a hole is formed in the arc-shaped fixed frame 1.7 for laying a 40-mesh second gauze 1.6.
The inner box body 1.3 is made of transparent PVC, the size of the inner box body is 103.00mm, the size of the inner box body is 120.00mm, the thickness of the wall and the box bottom is 3mm, the inner box body is provided with an inner box ventilation hole 1.2 and an inner box operation hole 1.4 which have the same diameter at a position 7mm away from the box bottom, and the size of the inner box operation hole 1.4 is consistent with the outer size of the inner box ventilation hole 1.2;
the outer box body 2.1 is made of transparent PVC, the size r is 106.00mm, h is 120.00mm, the wall thickness is 3mm, the outer box body is provided with an outer box ventilation hole 2.2 and an outer box operation hole 2.4 which are opposite to each other on the same diameter at a position 10mm away from the box bottom, and the size of the outer box operation hole 2.4 is consistent with the outer size of the outer box ventilation hole 2.2; the operation hole is unanimous with the ventilation hole size, has reduced the work of distinguishing the aperture in the use, and two operation holes are located same diameter simultaneously, and interior box ventilation hole can coincide with outer box operation hole when rotatory adjustment closed condition, and outer box ventilation hole can coincide with interior box operation hole, has increased the air permeability of box body.
The base 2.3 material be transparent PVC, including r 106.00mm, the circular chassis 2.5 that thickness is 3mm, be equipped with the ring shape spike 2.6 of height 3mm on the circular chassis 2.5 outer circumference. The setting of ring shape spike 2.6 can be convenient for fixed iron sheet 5.2 place, and it makes fixed tray 5 remove to avoid the maloperation to move fixed iron sheet 5.2.
The bottle body 4.2 is transparent PET, the size r is 9mm, h is 50mm of cylinder shape, the bottom thickness is 1mm, the wall thickness is 1mm, and the bottle top is provided with a bottle body thread 4.4 with the length of 6 mm; the egg collecting holes 4.1 are small holes with r being 0.5mm, 4 rows are distributed from the position 15mm away from the bottom of the bottle, and 20 egg collecting holes are formed.
The lid 4.3 material be PET, the size be r 11mm, h become 8mm cylindrical, from the lid top distribution 6 mm's bottle lid screw thread 4.5, closely spiral with body 4.2.
The edible plate 3 is made of PET, has a cylindrical shape with the size r being 11mm, the size h being 30mm, the bottom thickness being 1mm and the wall thickness being 1 mm. The size of the food tray 3 can be similar to that of the storage hole 5.4, so that the food tray can be taken out conveniently during operation and can also be placed upside down.
The object placing hole 5.4 has a radius of 10mm and a depth of 4mm, and a concentric magnet positioning hole 5.1 is arranged at the center of the circle;
the magnet positioning hole 5.1 is a hole with r being 2.5mm and h being 2mm, and can be closely connected with the magnet 5.3.
The magnet 5.3 is a cylindrical permanent magnet, which has a size r of 2.5mm and h of 2mm, and is attracted with the fixed iron sheet 5.2 for fixing.
The fixed magnet 5.2 is made of 204 series stainless steel, and has the size r of 50mm and the size delta of 1 mm.
The method for raising the adult fruit fly insects and measuring the biological parameters by using the device for raising the adult fruit fly insects and measuring the biological parameters comprises the following steps:
taking out the fixed tray 5, clamping a magnet 5.3 into a magnet positioning hole 5.1, plugging wet cotton balls with water, fruit fly feed and honey water with the mass content of 15% into the food tray 3, and then placing the food tray 3 into the placing hole 5.4;
taking out the egg taking device 4, pouring mango juice for inducing spawning into the egg taking device, enabling the liquid level to be 4.15mm away from the egg taking hole at the lower end, screwing a cover 4.3, and placing the egg taking device in an object placing hole 5.4;
taking out the inner box 1, and clamping the ventilation net into the ventilation hole 1.2 of the inner box;
taking out the outer box 2, and clamping the ventilation net into the ventilation hole 2.2 of the outer box;
putting the assembled inner box 1 into the assembled outer box 2, and putting a fixed tray 5 filled with a food tray 3 and an egg taking device 4 into the inner box 1;
step six, covering the inner box net cover 1.1, placing the fixed iron sheet 5.2 at the bottom of the outer box 2, and moving the fixed iron sheet 5.2 to adjust the position of the fixed tray 5 to the center through magnetic force;
step seven, rotating the outer box 2, adjusting the positions of the inner box operation hole 1.4 and the outer box operation hole 2.4, leading the eclosion female and male trypetid in the same day into the outer box operation hole after the two are superposed, rotating the outer box 2 again, leading the inner box operation hole 1.4 to be superposed with the outer box ventilation hole 2.2, and then placing the device in a position with L: D being 14: 10, RH 70%, T25 deg.C artificial climate box;
and step eight, observing the survival state of the female and male insects in the recording device every day, moving a fixed iron sheet 5.2, adjusting the position of the fixed tray 5 to the positions of the inner box operation hole 1.4 and the outer box operation hole 2.4 through magnetic force, replacing a fresh cotton ball and feed, pouring mango juice in the egg collector 4 out, filtering the mango juice by using a 80-mesh gauze, counting the number of eggs until the adult insects die, and measuring various biological parameters obtained in the adult stage of the fruit fly.
The specific operation example is as follows:
the citrus fruit fly 3 days after eclosion was selected as the test fruit fly, collected from apple orchard in Showa City of Yunnan province in 2021 year, then placed in insect-raising room, and subcultured at 24 + -1 deg.C and RH (70 + -5)% for 5 generations. Adopt this device, traditional glass raises jar and raises the experiment.
Test one: the feeding test was performed using 2 feeding devices, each method was repeated 4 times, and the time from taking out the device to completion of observation and change of food, cotton ball with water, and egg taking device, etc. was recorded.
And (2) test II: feeding experiments were carried out using 2 feeding devices, each method was divided into 4 groups, 5 devices were used per group, 1 male and female insect was introduced into each of the 2 devices, feeding was continued for 20 days, and the escape number of bactrocera dorsalis was recorded.
And (3) test III: feeding experiments are carried out by respectively using 2 feeding devices, each method is divided into 4 groups, each group uses 5 devices, 1 male and female insect is respectively introduced into each of the 2 devices, feeding is carried out for 20 days continuously, and the times of shaking and clamping of the bactrocera dorsalis due to transfer of the containers are recorded.
And (4) testing: the feeding experiment was carried out using 2 feeding devices, each method was divided into 4 groups, 5 devices were used for each group, 1 male and female insects were introduced into each of the 2 devices, feeding was continued for 20 days, and the number of citrus fruit fly deaths was recorded.
(1) Test of efficiency of two feeding devices for feeding bactrocera dorsalis imagoes
TABLE 1 comparison of feeding efficiency of different feeding devices
Note: the times in the table are mean. + -. standard error. The lower case letters are identical indicating that the difference is not significant, and the differences indicate that the difference is significant. Significance of difference was calculated using DPS 7.5.
The feeding efficiency of the different feeding devices is shown in table 1. The observation time difference between the glass feeding tank and the invention is not obvious, and the time for changing food is obviously shorter than that of the glass feeding tank (P is less than 0.05).
(2) Comparative experiment for preventing escape effect of citrus fruit fly raised by two raising devices
TABLE 2 escape number of Bactrocera dorsalis adults during rearing with different rearing devices
Note: mean ± standard error of the escape number of the adult insects in the table. The lower case letters are identical indicating that the difference is not significant, and the differences indicate that the difference is significant. Significance of difference was calculated using DPS 7.5.
The escape numbers of bactrocera dorsalis adults during the rearing process with different rearing devices are shown in table 2. The escape number of the bactrocera dorsalis fed by the method is 0.50, which is obviously lower than that of the bactrocera dorsalis fed by a glass feeding tank.
(3) Comparative test of insect clamping times of citrus fruit fly container fed by two feeding devices
TABLE 3 frequency of the container for catching the imago of Bactrocera dorsalis during the raising process of different raising devices
Note: the number of beetles in the table is the mean value. + -. standard error. The lower case letters are identical indicating that the difference is not significant, and the differences indicate that the difference is significant. Significance of difference was calculated using DPS 7.5.
The number of times of the bactrocera dorsalis adults caught by the movement of the container due to transfer in the rearing process of the different rearing devices is shown in table 3. The frequency of the insects which are caught by the device is 8.25 times, which is obviously lower than the frequency of the insects which are fed by the glass feeding tank.
(4) Feeding experiment for feeding bactrocera dorsalis with two feeding devices
TABLE 4 number of deaths of bactrocera dorsalis adults on different rearing devices
Note: the number of dead insects in the table is the mean. + -. standard error. The lower case letters are identical indicating that the difference is not significant, and the differences indicate that the difference is significant. Significance of difference was calculated using DPS 7.5.
The death number difference of the bactrocera dorsalis adults in the feeding process of different feeding devices is obvious, and the death number of the bactrocera dorsalis adults fed in the glass feeding tank is higher than that of the bactrocera dorsalis adults fed in the feeding process of the invention.
The test results show that the device and the method adopted by the invention are obviously superior to those of a glass feeding tank. The result of the feeding test of feeding the bactrocera dorsalis by the two feeding devices shows that the device provides a good ventilating environment for the survival of the bactrocera dorsalis and reduces the number of dead insects. And this device lower part opening can prevent effectively that the fruit fly adult from gathering in the opening part because of tendency nature, interior outer box rotation type switch and fixed tray design can facilitate for daily fruit fly adult raises the management, and fixed tray cooperation magnet and fixed iron sheet can effectually avoid rocking of splendid attire container to reduce the number of times of card worm. The opening of the traditional glass feeding tank is upward, the trypetid pests are gathered at the position frequently due to tendency, the trypetid pests can escape during operation in daily management, and the containing container is not fixed, so that the containing container shakes.
Although the device, method and results for raising adult fruit fly insects and measuring biological parameters are described in detail above, other types of insects can be raised by the method and structure of the present invention with only slight adjustments, such as the size of the raising box, the number of meshes of the screen, etc.
The foregoing is merely a specific embodiment of the present invention, and common general knowledge of known specific structures and characteristics in the schemes has not been described herein. It should be noted that, for those skilled in the art, without departing from the structure of the present invention, several changes and modifications can be made, which should also be regarded as the protection scope of the present invention, and these will not affect the effect of the implementation of the present invention and the practicability of the patent. The scope of the claims of the present application shall be determined by the contents of the claims, and the description of the embodiments and the like in the specification shall be used to explain the contents of the claims.
Claims (10)
1. The device for raising adult fruit fly insects and measuring biological parameters is characterized by comprising an inner box (1), an outer box (2), a feeding tray (3), an egg taking device (4) and a fixed tray (5);
the inner box (1) is arranged in the outer box (2), and the fixed tray (5) is arranged in the outer box (2);
the inner box (1) comprises an inner box net cover (1.1), an inner box ventilation hole (1.2), an inner box body (1.3) and an inner box operation hole (1.4), wherein the inner box ventilation hole (1.2) and the inner box operation hole (1.4) are oppositely arranged on the side wall of the inner box body (1.3);
the outer box (2) comprises an outer box body (2.1), an outer box ventilation hole (2.2), an outer box operation hole (2.4) and a base (2.3), wherein the outer box ventilation hole (2.2) and the outer box operation hole (2.4) are oppositely arranged on the side wall of the outer box body (2.1), and the outer box body (2.1) is tightly bonded with the base (2.3);
the ventilation holes (1.2) of the inner box and the ventilation holes (2.2) of the outer box are provided with ventilation nets;
the egg taking device (4) comprises a bottle body (4.2) and a cover (4.3), and an egg taking hole (4.1) is formed in the bottle body (4.2); the bottle body (4.2) is connected with the cover (4.3) through threads;
the fixed tray (5) comprises an object placing hole (5.4);
a plurality of magnet positioning holes (5.1) are arranged on the fixing tray (5), and magnets (5.3) are arranged in the magnet positioning holes (5.1);
the bottom of the box is provided with a fixed iron sheet (5.2), and the fixed iron sheet (5.2) is positioned outside the bottom of the outer box (2).
2. The device for raising adult fruit fly insects and measuring biological parameters as claimed in claim 1, wherein said inner box net cover (1.1) is made of transparent PVC and has a radius of 101.50mm, a circular opening is formed in the center of the inner box net cover and has a radius of 78.50mm, and a first 40-mesh gauze (1.5) is laid in the circular opening.
3. The device for raising adult fruit fly insects and measuring biological parameters as claimed in claim 1, wherein said ventilation net comprises an arc-shaped fixed frame (1.7), and said arc-shaped fixed frame (1.7) is perforated with a 40-mesh second gauze (1.6).
4. The device for raising adult fruit fly insects and measuring biological parameters as claimed in claim 1, wherein the inner box body (1.3) is made of transparent PVC, has a cylindrical shape with r being 103.00mm and h being 120.00mm, has a wall and a box bottom thickness of 3mm, and is provided with an inner box ventilation hole (1.2) and an inner box operation hole (1.4) with the same diameter at a position 7mm away from the box bottom, and the size of the inner box operation hole (1.4) is consistent with the outer size of the inner box ventilation hole (1.2);
the outer box body (2.1) is made of transparent PVC, the size r is 106.00mm, h is 120.00mm, the wall thickness is 3mm, the outer box body is provided with an outer box ventilation hole (2.2) and an outer box operation hole (2.4) which are opposite to each other on the same diameter at a position 10mm away from the box bottom, and the size of the outer box operation hole (2.4) is consistent with the outer size of the outer box ventilation hole (2.2);
the base (2.3) material be transparent PVC, including r 106.00mm, the circular chassis (2.5) that thickness is 3mm, be equipped with ring shape spike (2.6) of height 3mm on the outer circumference of circular chassis (2.5).
5. The device for raising adult fruit fly insects and measuring biological parameters as claimed in claim 1, wherein said bottle body (4.2) is transparent PET, and has a cylindrical shape with r 9mm, h 50mm, a bottom thickness of 1mm, a wall thickness of 1mm, and a bottle top with a 6mm long screw thread (4.4) on the bottle body; the egg collecting holes (4.1) are small holes with the r being 0.5mm, 4 rows are distributed from the position 15mm away from the bottom of the bottle, and 20 egg collecting holes are formed;
the cap (4.3) is made of PET, is cylindrical with the size of r being 11mm and h being 8mm, is provided with 6mm bottle cap threads (4.5) distributed from the top of the cap and is tightly screwed with the bottle body (4.2).
6. The device for raising adult fruit fly insects and measuring biological parameters as claimed in claim 1, wherein said feeding plate (3) is made of PET, and has a cylindrical shape with r-11 mm, h-30 mm, a bottom thickness of 1mm and a wall thickness of 1 mm.
7. The device for raising adult fruit fly insects and measuring biological parameters as claimed in claim 1, wherein said fixing tray (5) is made of transparent PVC, the size r is 50mm, h is 6mm, 4 object placing holes (5.4) are provided on four vertexes of a square with the length of 50mm and the center of the diagonal line is coincident with the center of the circle, and 5 magnet positioning holes (5.1) are provided on the four vertexes and the intersection point of the diagonal line of the square;
the radius of the object placing hole (5.4) is 10mm, the depth is 4mm, and a concentric magnet positioning hole (5.1) is arranged at the center of the object placing hole;
the magnet positioning hole (5.1) is a hole with r being 2.5mm and h being 2mm and is tightly connected with the magnet (5.3).
8. The device for raising adult fruit fly insects and measuring biological parameters as claimed in claim 7, wherein said magnet (5.3) is a cylindrical permanent magnet, and a cylinder with r-2.5 mm and h-2 mm is attracted to the fixing iron plate (5.2) for fixing.
9. The device for raising adult fruit fly insects and measuring biological parameters as claimed in claim 8, wherein said fixing magnet (5.2) is made of 204 series stainless steel, and has a size of r-50 mm and δ -1 mm.
10. A method for raising adult fruit fly insects and measuring biological parameters, which comprises the steps of using the apparatus for raising adult fruit fly insects and measuring biological parameters according to claims 1 to 9, comprising:
taking out the fixed tray (5), clamping a magnet (5.3) into a magnet positioning hole (5.1), plugging wet cotton balls with water, fruit fly feed and honey water with the mass content of 15% into the food tray (3), and then putting the food tray (3) into the placing hole (5.4);
taking out the egg taking device (4), pouring mango juice for inducing spawning into the egg taking device, enabling the liquid level to be about 5mm away from the egg taking hole (4.1) at the lower end, screwing a cover (4.3) and placing the egg taking device in the object placing hole (5.4);
taking out the inner box (1), and clamping the ventilation net into the ventilation hole (1.2) of the inner box;
taking out the outer box (2), and clamping the ventilation net into the ventilation hole (2.2) of the outer box;
putting the assembled inner box (1) into the assembled outer box (2), and putting a fixed tray (5) filled with a food tray (3) and an egg taking device (4) into the inner box (1);
step six, covering the inner box net cover (1.1), placing the fixed iron sheet (5.2) at the bottom of the outer box (2), and moving the fixed iron sheet (5.2) to adjust the position of the fixed tray (5) to the center through magnetic force;
seventhly, rotating the outer box (2), adjusting the positions of the inner box operation hole (1.4) and the outer box operation hole (2.4), leading the eclosion female and male fruit flies in the day after the inner box operation hole and the outer box operation hole are overlapped, rotating the outer box (2) again to ensure that the inner box operation hole (1.4) and the outer box ventilation hole (2.2) are overlapped, and then placing the device in a position where L is 14: 10, RH 70%, T25 deg.C artificial climate box;
and step eight, observing the survival state of the female and male insects in the recording device every day, moving a fixed iron sheet (5.2) to adjust the position of a fixed tray (5) to the positions of an inner box operation hole (1.4) and an outer box operation hole (2.4) through magnetic force, replacing a fresh cotton ball and feed, pouring mango juice in an egg collector (4), filtering the mango juice by using a 80-mesh gauze, counting the number of eggs until the adult insects die, and measuring various biological parameters obtained in the adult period of the fruit fly.
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