CN1906294B - 改良的蛋白表达系统 - Google Patents

改良的蛋白表达系统 Download PDF

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Publication number
CN1906294B
CN1906294B CN2004800407027A CN200480040702A CN1906294B CN 1906294 B CN1906294 B CN 1906294B CN 2004800407027 A CN2004800407027 A CN 2004800407027A CN 200480040702 A CN200480040702 A CN 200480040702A CN 1906294 B CN1906294 B CN 1906294B
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gene
pseudomonas
cell
nucleic acid
auxotrophic
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Chinese (zh)
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CN1906294A (zh
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J·C·施奈德
L·C·丘
A·K·巴杰利
T·M·拉姆塞耶尔
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Peliken Technology Holdings Ltd
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Dow Global Technologies LLC
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/0004—Oxidoreductases (1.)
    • C12N9/0012—Oxidoreductases (1.) acting on nitrogen containing compounds as donors (1.4, 1.5, 1.6, 1.7)
    • C12N9/0026—Oxidoreductases (1.) acting on nitrogen containing compounds as donors (1.4, 1.5, 1.6, 1.7) acting on CH-NH groups of donors (1.5)
    • C12N9/0028—Oxidoreductases (1.) acting on nitrogen containing compounds as donors (1.4, 1.5, 1.6, 1.7) acting on CH-NH groups of donors (1.5) with NAD or NADP as acceptor (1.5.1)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/20—Bacteria; Culture media therefor
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/52—Genes encoding for enzymes or proenzymes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/74—Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora
    • C12N15/78—Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora for Pseudomonas
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/88—Lyases (4.)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P21/00—Preparation of peptides or proteins
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P21/00—Preparation of peptides or proteins
    • C12P21/02—Preparation of peptides or proteins having a known sequence of two or more amino acids, e.g. glutathione
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y305/00—Hydrolases acting on carbon-nitrogen bonds, other than peptide bonds (3.5)
    • C12Y305/05—Hydrolases acting on carbon-nitrogen bonds, other than peptide bonds (3.5) in nitriles (3.5.5)
    • C12Y305/05001—Nitrilase (3.5.5.1)

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  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Genetics & Genomics (AREA)
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  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Biotechnology (AREA)
  • Biomedical Technology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Molecular Biology (AREA)
  • Microbiology (AREA)
  • Medicinal Chemistry (AREA)
  • General Chemical & Material Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Plant Pathology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Virology (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Enzymes And Modification Thereof (AREA)
CN2004800407027A 2003-11-19 2004-11-19 改良的蛋白表达系统 Expired - Lifetime CN1906294B (zh)

Applications Claiming Priority (5)

Application Number Priority Date Filing Date Title
US52342003P 2003-11-19 2003-11-19
US60/523,420 2003-11-19
US53714704P 2004-01-16 2004-01-16
US60/537,147 2004-01-16
PCT/US2004/038884 WO2005052151A1 (en) 2003-11-19 2004-11-19 Improved protein expression systems

Publications (2)

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CN1906294A CN1906294A (zh) 2007-01-31
CN1906294B true CN1906294B (zh) 2013-09-11

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US (2) US20050186666A1 (enExample)
EP (1) EP1692282B1 (enExample)
JP (2) JP5087741B2 (enExample)
KR (1) KR101237651B1 (enExample)
CN (1) CN1906294B (enExample)
AU (1) AU2004293810B2 (enExample)
BR (1) BRPI0416744A (enExample)
CA (1) CA2545610C (enExample)
MX (1) MXPA06005634A (enExample)
WO (1) WO2005052151A1 (enExample)

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN104004760A (zh) * 2014-06-12 2014-08-27 华东理工大学 一种用于在米曲霉细胞分泌表达外源蛋白的表达设备及其米曲霉基因工程菌

Families Citing this family (59)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US9453251B2 (en) 2002-10-08 2016-09-27 Pfenex Inc. Expression of mammalian proteins in Pseudomonas fluorescens
CA2545610C (en) * 2003-11-19 2014-03-25 Dow Global Technolgies Inc. Auxotrophic pseudomonas fluorescens bacteria for recombinant protein expression
AU2005269527B2 (en) 2004-07-26 2011-12-01 Pfenex Inc. Process for improved protein expression by strain engineering
ES2562664T3 (es) * 2005-10-06 2016-03-07 Advanced Accelerator Applications S.A. Nuevo sistema de selección
US8043833B2 (en) * 2005-10-31 2011-10-25 Novo Nordisk A/S Expression of soluble therapeutic proteins
GB0602173D0 (en) 2006-02-03 2006-03-15 Avecia Ltd Expression system
JP2009534034A (ja) 2006-04-19 2009-09-24 ノヴォ ノルディスク アクティーゼルスカブ 原核微生物におけるｏ−グリコシル化治療用タンパク質の発現
JP2009538622A (ja) * 2006-05-30 2009-11-12 ダウ グローバル テクノロジーズ インコーポレイティド コドン最適化法
KR100824541B1 (ko) * 2007-02-26 2008-04-24 고려대학교 산학협력단 항산화 효소의 과발현을 통해 환경오염물질 분해능이향상된 균주
US9580719B2 (en) 2007-04-27 2017-02-28 Pfenex, Inc. Method for rapidly screening microbial hosts to identify certain strains with improved yield and/or quality in the expression of heterologous proteins
EP2142651B1 (en) 2007-04-27 2013-05-22 Pfenex Inc. Method for rapidly screening microbial hosts to identify certain strains with improved yield and/or quality in the expression of heterologous proteins
CN101918580A (zh) * 2007-11-29 2010-12-15 诺维信公司 合酶抑制剂筛选方法
WO2009073551A2 (en) * 2007-11-30 2009-06-11 Scarab Genomics Llc Lac expression system
WO2010008764A1 (en) * 2008-06-23 2010-01-21 Dow Global Technologies Inc. Pseudomonas fluorescens strains for production of extracellular recombinant protein
JP2010017131A (ja) * 2008-07-10 2010-01-28 Mitsui Eng & Shipbuild Co Ltd モーレラ属細菌及びプライマー
US8357503B2 (en) 2008-08-29 2013-01-22 Bunge Oils, Inc. Hydrolases, nucleic acids encoding them and methods for making and using them
US8153391B2 (en) 2008-08-29 2012-04-10 Bunge Oils, Inc. Hydrolases, nucleic acids encoding them and methods for making and using them
US8198062B2 (en) * 2008-08-29 2012-06-12 Dsm Ip Assets B.V. Hydrolases, nucleic acids encoding them and methods for making and using them
US8623652B2 (en) * 2009-04-06 2014-01-07 Lucigen Corporation Host-vector system for cloning and expressing genes
EP2430164B1 (en) * 2009-05-11 2017-07-19 Pfenex, Inc. Production of recombinant proteins utilizing non-antibiotic selection methods and the incorporation of non-natural amino acids therein
UA111708C2 (uk) 2009-10-16 2016-06-10 Бандж Ойлз, Інк. Спосіб рафінування олії
UA109884C2 (uk) 2009-10-16 2015-10-26 Поліпептид, що має активність ферменту фосфатидилінозитол-специфічної фосфоліпази с, нуклеїнова кислота, що його кодує, та спосіб його виробництва і застосування
AU2011223627B2 (en) 2010-03-04 2015-06-18 Pfenex Inc. Method for producing soluble recombinant interferon protein without denaturing
MX343356B (es) 2010-03-30 2016-11-03 Pfenex Inc * Toxinas proteicas recombinantes de alto nivel de expresion.
NZ603033A (en) 2010-04-01 2014-06-27 Pfenex Inc Methods for g-csf production in a pseudomonas host cell
CA2804713C (en) 2010-07-12 2021-04-27 Universiteit Gent Metabolically engineered organisms for the production of added value bio-products
CA2807774C (en) 2010-08-10 2020-10-27 The Governing Council Of The University Of Toronto Specific active site inhibitors of enzymes or substrate binding partners and methods of producing same
PL222067B1 (pl) * 2011-08-11 2016-06-30 Inst Biotechnologii I Antybiotyków Kaseta ekspresyjna, zastosowanie kasety ekspresyjnej, wektor ekspresyjny, komórka prokariotycznego gospodarza zawierająca wektor ekspresyjny, szczep bakteryjny oraz sposób wytwarzania polipeptydu
KR20140140615A (ko) 2012-03-30 2014-12-09 베레늄 코포레이션 셀룰라제를 코딩하는 유전자
US9169304B2 (en) 2012-05-01 2015-10-27 Pfenex Inc. Process for purifying recombinant Plasmodium falciparum circumsporozoite protein
WO2014047625A1 (en) 2012-09-24 2014-03-27 Montana State University Recombinant lactococcus lactis expressing escherichia coli colonization factor antigen i (cfa/i) fimbriae and their methods of use
JP6448909B2 (ja) * 2013-03-01 2019-01-09 学校法人関西学院 目的遺伝子を欠損した酢酸菌の製造方法及びその利用
GB201308828D0 (en) 2013-03-12 2013-07-03 Verenium Corp Phytase
GB201308853D0 (en) 2013-03-12 2013-07-03 Verenium Corp Genes encoding xylanase
GB201308843D0 (en) 2013-03-14 2013-07-03 Verenium Corp Phytase formulation
KR20160033711A (ko) 2013-07-25 2016-03-28 바스프 엔자임스 엘엘씨 피타아제
WO2015184526A1 (en) 2014-06-06 2015-12-10 The Hospital For Sick Children Soluble bacterial and fungal proteins and methods and uses thereof in inhibiting and dispersing biofilm
IL249749B2 (en) 2014-06-27 2023-03-01 Nat Res Council Canada Cannabichromic acid synthase from Cannabis sativa
EP3164501A4 (en) 2014-07-02 2018-01-17 Sutro Biopharma, Inc. High growth capacity auxotrophic escherichia coli and methods of use
NZ732400A (en) 2014-12-01 2021-07-30 Pfenex Inc Fusion partners for peptide production
PL3253406T3 (pl) 2015-02-05 2024-04-29 Canem Holdings, Llc Kompozycje do leczenia ziarniniakowatości z zapaleniem naczyń
KR20170068304A (ko) * 2015-12-09 2017-06-19 삼성전자주식회사 대장균 및 코마가타에이박터 속 세포에서 복제가능한 벡터, 그를 포함한 세포, 및 그를 이용하는 방법
WO2019063613A1 (en) 2017-09-26 2019-04-04 Dsm Ip Assets B.V. ENZYMATIC ELIMINATION OF CHLOROPHYLL SUBSTRATES FROM TRIACYLGLYCEROL OILS
JP7358347B2 (ja) 2017-10-27 2023-10-10 フェネックス インク． 組み換えＥ．ｃｏｌｉアスパラギナーゼの製造のための方法
BR112020008369A2 (pt) 2017-10-27 2020-11-03 Pfenex Inc. sequências líder bacterianas para a expressão de proteína periplásmica
AU2018354067B2 (en) 2017-10-27 2023-03-30 Pfenex Inc. Method for production of recombinant Erwinia asparaginase
CN108504677B (zh) * 2018-03-06 2020-05-12 上海晶诺生物科技有限公司 一个用于构建谷氨酸基因家族缺失结核分枝杆菌的重组tm4噬菌体文库及其应用
US11098265B2 (en) 2019-03-27 2021-08-24 Bunge Global Innovation, Llc Silica adsorbent treatment for removal of chlorophyll derivatives from triacylglycerol-based oils
WO2020243642A2 (en) * 2019-05-31 2020-12-03 The Trustees Of Columbia University In The City Of New York Multiply auxotrophic cell line for the production of recombinant proteins and methods thereof
TW202122582A (zh) * 2019-08-26 2021-06-16 美商航海家醫療公司 病毒蛋白之控制表現
CN111041029B (zh) * 2019-12-23 2022-05-10 中国科学院天津工业生物技术研究所 一种强启动子及其在产维生素b12菌株中的应用
CN113025601B (zh) * 2019-12-25 2024-06-21 上海奥博生物医药股份有限公司 腈水解酶启动子优化表达及应用
US12098372B2 (en) 2019-12-30 2024-09-24 Eligo Bioscience Microbiome modulation of a host by delivery of DNA payloads with minimized spread
US11746352B2 (en) 2019-12-30 2023-09-05 Eligo Bioscience Microbiome modulation of a host by delivery of DNA payloads with minimized spread
CN112080451A (zh) * 2020-07-13 2020-12-15 宁波大学 一种嗜酸乳杆菌的食品级基因表达体系及其制备方法和应用
WO2022144381A1 (en) 2020-12-30 2022-07-07 Eligo Bioscience Microbiome modulation of a host by delivery of dna payloads with minimized spread
US20220313798A1 (en) 2021-03-30 2022-10-06 Jazz Pharmaceuticals Ireland Ltd. Dosing of recombinant l-asparaginase
KR20240020271A (ko) 2021-05-12 2024-02-14 엘리고 바이오사이언스 생산 박테리아 세포 및 제조 방법에서 이의 용도
WO2024211833A2 (en) 2023-04-05 2024-10-10 Primrose Bio, Inc. Methods and compositions for nucleic acid synthesis

Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0972838A1 (en) * 1998-07-15 2000-01-19 Roche Diagnostics GmbH Escherichia coli host/vector system based on antibiotic-free selection by complementation of an auxotrophy
WO2003089455A2 (en) * 2002-04-22 2003-10-30 Dow Global Technologies Inc. Low-cost production of peptides

Family Cites Families (28)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4511503A (en) 1982-12-22 1985-04-16 Genentech, Inc. Purification and activity assurance of precipitated heterologous proteins
US4755465A (en) 1983-04-25 1988-07-05 Genentech, Inc. Secretion of correctly processed human growth hormone in E. coli and Pseudomonas
US4680264A (en) 1983-07-01 1987-07-14 Lubrizol Genetics, Inc. Class II mobilizable gram-negative plasmid
US5281532A (en) 1983-07-27 1994-01-25 Mycogen Corporation Pseudomas hosts transformed with bacillus endotoxin genes
DK594084A (da) * 1984-12-12 1986-06-13 Novo Industri As Fremgangsmaade til stabilisering af extra-chromosomale elementer i bakterier under dyrkning
US4695462A (en) 1985-06-28 1987-09-22 Mycogen Corporation Cellular encapsulation of biological pesticides
US4695455A (en) 1985-01-22 1987-09-22 Mycogen Corporation Cellular encapsulation of pesticides produced by expression of heterologous genes
GB8517071D0 (en) 1985-07-05 1985-08-14 Hoffmann La Roche Gram-positive expression control sequence
US5232840A (en) * 1986-03-27 1993-08-03 Monsanto Company Enhanced protein production in bacteria by employing a novel ribosome binding site
US5128130A (en) 1988-01-22 1992-07-07 Mycogen Corporation Hybrid Bacillus thuringiensis gene, plasmid and transformed Pseudomonas fluorescens
US5055294A (en) 1988-03-03 1991-10-08 Mycogen Corporation Chimeric bacillus thuringiensis crystal protein gene comprising hd-73 and berliner 1715 toxin genes, transformed and expressed in pseudomonas fluorescens
US5169760A (en) * 1989-07-27 1992-12-08 Mycogen Corporation Method, vectors, and host cells for the control of expression of heterologous genes from lac operated promoters
JP2600090B2 (ja) 1991-04-19 1997-04-16 農林水産省農業環境技術研究所長 宿主ベクター系
US6566121B1 (en) * 1991-06-13 2003-05-20 Albert Einstein College Of Medicine Of Yeshiva University Insertional mutations in mycobacteria
US5691185A (en) * 1993-10-08 1997-11-25 Chr. Hansen A/S Lactic acid bacterial suppressor mutants and their use as selective markers and as means of containment in lactic acid bacteria
US5527883A (en) 1994-05-06 1996-06-18 Mycogen Corporation Delta-endotoxin expression in pseudomonas fluorescens
WO1997004110A1 (en) 1995-07-14 1997-02-06 Somatogen, Inc. Methods for increasing protein expression
AU1467399A (en) 1997-11-20 1999-06-15 William Marsh Rice University Lactose repressor proteins with altered ligand responsivity
US7390645B2 (en) * 1997-11-20 2008-06-24 William Marsh Rice University Lactose repressor proteins with increased operator DNA binding affinity
DE19801120A1 (de) * 1998-01-15 1999-07-22 Basf Ag Orotidin-5'-Phosphatdecarboxylase-Gen, Genkonstrukt enthaltend dieses Gen und seine Verwendung
JP4320689B2 (ja) 1998-06-16 2009-08-26 株式会社力王 履物用先芯
US6291245B1 (en) * 1998-07-15 2001-09-18 Roche Diagnostics Gmbh Host-vector system
US6413768B1 (en) * 1998-12-02 2002-07-02 University Of Maryland Expression plasmids
EP1373297A4 (en) * 2001-03-05 2005-09-21 Univ Virginia A LAC OPERATOR REPRESSOR SYSTEM
WO2003062399A2 (en) * 2002-01-23 2003-07-31 Diversa Corporation Biocatalytic reduction of nitro groups
AU2002306484A1 (en) * 2002-02-13 2003-09-04 Dow Global Technologies Inc. Over-expression of extremozyme genes in pseudomonads and closely related bacteria
DE10214406A1 (de) * 2002-03-30 2003-10-09 Nutrinova Gmbh Verfahren und Marker zur einfachen Transformation und Selektion von rekombinanten Protisten
CA2545610C (en) 2003-11-19 2014-03-25 Dow Global Technolgies Inc. Auxotrophic pseudomonas fluorescens bacteria for recombinant protein expression

Patent Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0972838A1 (en) * 1998-07-15 2000-01-19 Roche Diagnostics GmbH Escherichia coli host/vector system based on antibiotic-free selection by complementation of an auxotrophy
WO2003089455A2 (en) * 2002-04-22 2003-10-30 Dow Global Technologies Inc. Low-cost production of peptides

Non-Patent Citations (2)

* Cited by examiner, † Cited by third party
Title
EL-Sayed.Quorum-sensing-dependent regulation of biosynthesis of thepolyketide antibiotic mupirocin in pseudomonas fluorescensNCIMB 10586.microbiology147 8.2001,147(8),全文.
EL-Sayed.Quorum-sensing-dependent regulation of biosynthesis of thepolyketide antibiotic mupirocin in pseudomonas fluorescensNCIMB 10586.microbiology147 8.2001,147(8),全文. *

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN104004760A (zh) * 2014-06-12 2014-08-27 华东理工大学 一种用于在米曲霉细胞分泌表达外源蛋白的表达设备及其米曲霉基因工程菌

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Publication number Publication date
KR101237651B1 (ko) 2013-02-27
WO2005052151A1 (en) 2005-06-09
KR20060132587A (ko) 2006-12-21
JP5087741B2 (ja) 2012-12-05
BRPI0416744A (pt) 2007-01-16
US20090325230A1 (en) 2009-12-31
JP2007511238A (ja) 2007-05-10
EP1692282B1 (en) 2012-08-08
US20050186666A1 (en) 2005-08-25
CN1906294A (zh) 2007-01-31
EP1692282A1 (en) 2006-08-23
AU2004293810A1 (en) 2005-06-09
CA2545610C (en) 2014-03-25
AU2004293810B2 (en) 2010-07-01
MXPA06005634A (es) 2006-08-23
JP2012100669A (ja) 2012-05-31
CA2545610A1 (en) 2005-06-09
US8288127B2 (en) 2012-10-16
EP1692282A4 (en) 2008-04-02

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