CN1764842B - 微芯片、用于提取核酸的试剂盒以及核酸的提取方法 - Google Patents
微芯片、用于提取核酸的试剂盒以及核酸的提取方法 Download PDFInfo
- Publication number
- CN1764842B CN1764842B CN2004800082843A CN200480008284A CN1764842B CN 1764842 B CN1764842 B CN 1764842B CN 2004800082843 A CN2004800082843 A CN 2004800082843A CN 200480008284 A CN200480008284 A CN 200480008284A CN 1764842 B CN1764842 B CN 1764842B
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- China
- Prior art keywords
- microchannel
- microchip
- nucleic acid
- microballon
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- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Fee Related
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Classifications
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
- B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
- B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
- B01L3/5027—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
- B01L3/502746—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip characterised by the means for controlling flow resistance, e.g. flow controllers, baffles
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
- B01J19/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
- B01J19/0093—Microreactors, e.g. miniaturised or microfabricated reactors
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
- B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
- B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
- B01L3/5027—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
- B01L3/502707—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip characterised by the manufacture of the container or its components
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
- C12N15/1003—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor
- C12N15/1006—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor by means of a solid support carrier, e.g. particles, polymers
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6806—Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
- B01J2219/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
- B01J2219/00781—Aspects relating to microreactors
- B01J2219/00783—Laminate assemblies, i.e. the reactor comprising a stack of plates
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L2200/00—Solutions for specific problems relating to chemical or physical laboratory apparatus
- B01L2200/06—Fluid handling related problems
- B01L2200/0647—Handling flowable solids, e.g. microscopic beads, cells, particles
- B01L2200/0668—Trapping microscopic beads
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L2300/00—Additional constructional details
- B01L2300/08—Geometry, shape and general structure
- B01L2300/0809—Geometry, shape and general structure rectangular shaped
- B01L2300/0816—Cards, e.g. flat sample carriers usually with flow in two horizontal directions
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L2400/00—Moving or stopping fluids
- B01L2400/08—Regulating or influencing the flow resistance
- B01L2400/084—Passive control of flow resistance
- B01L2400/086—Passive control of flow resistance using baffles or other fixed flow obstructions
Applications Claiming Priority (3)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
JP081605/2003 | 2003-03-24 | ||
JP2003081605 | 2003-03-24 | ||
PCT/JP2004/003998 WO2004086055A1 (ja) | 2003-03-24 | 2004-03-24 | マイクロチップ、核酸抽出用のキット及び核酸の抽出方法 |
Publications (2)
Publication Number | Publication Date |
---|---|
CN1764842A CN1764842A (zh) | 2006-04-26 |
CN1764842B true CN1764842B (zh) | 2012-01-11 |
Family
ID=33094898
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
CN2004800082843A Expired - Fee Related CN1764842B (zh) | 2003-03-24 | 2004-03-24 | 微芯片、用于提取核酸的试剂盒以及核酸的提取方法 |
Country Status (6)
Families Citing this family (17)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
JP2006029798A (ja) * | 2004-07-12 | 2006-02-02 | Hitachi Software Eng Co Ltd | 試薬を内蔵した高反応効率生体物質検査チップ |
US20090149345A1 (en) * | 2005-03-07 | 2009-06-11 | Kuraray Co., Ltd. | Microchannel array and method for producing the same, and blood measuring method employing it |
JP4904973B2 (ja) * | 2005-08-08 | 2012-03-28 | ソニー株式会社 | 核酸回収方法、核酸回収装置、並びに核酸供給装置 |
JP4657084B2 (ja) * | 2005-11-02 | 2011-03-23 | パナソニック株式会社 | 試料分析用ディスク |
JP2007147456A (ja) * | 2005-11-28 | 2007-06-14 | Seiko Epson Corp | マイクロ流体システム、試料分析装置、及び標的物質の検出または測定方法 |
US7608399B2 (en) | 2006-06-26 | 2009-10-27 | Blood Cell Storage, Inc. | Device and method for extraction and analysis of nucleic acids from biological samples |
JP4848896B2 (ja) * | 2006-08-31 | 2011-12-28 | 横河電機株式会社 | マイクロチップ |
JP4297148B2 (ja) | 2006-09-22 | 2009-07-15 | ソニー株式会社 | 核酸回収装置及び核酸回収方法 |
WO2009117167A1 (en) | 2008-01-02 | 2009-09-24 | Blood Cell Storage, Inc. | Devices and processes for nucleic acid extraction |
KR100945129B1 (ko) * | 2008-03-17 | 2010-03-02 | 고정문 | 디앤에이 정제용 칩 및 이를 이용한 정제 방법 |
WO2009150583A1 (en) * | 2008-06-10 | 2009-12-17 | Koninklijke Philips Electronics N.V. | Diagnostic device |
KR101563687B1 (ko) | 2008-09-02 | 2015-11-09 | 삼성전자주식회사 | 표적 분자 분리를 위한 미세유동 카트리지, 분리장치, 및 이를 이용한 표적 분자 분리 방법 |
CN103398936A (zh) * | 2009-07-07 | 2013-11-20 | 索尼公司 | 微流体装置 |
WO2012159063A2 (en) | 2011-05-19 | 2012-11-22 | Blood Cell Strorage, Inc. | Gravity flow fluidic device for nucleic acid extraction |
KR101428578B1 (ko) * | 2012-05-21 | 2014-08-12 | 한국과학기술원 | 미소입자 선택적 포획-회수 장치 |
CN108031457B (zh) * | 2017-12-22 | 2020-11-17 | 深圳逗点生物技术有限公司 | 一种吸附核酸的复合材料及其制备方法、吸附核酸的装置 |
IL263582A (en) | 2018-12-09 | 2020-06-30 | Vbact Ltd | A system for photographing and monitoring flows |
Citations (2)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US6319469B1 (en) * | 1995-12-18 | 2001-11-20 | Silicon Valley Bank | Devices and methods for using centripetal acceleration to drive fluid movement in a microfluidics system |
CN1355319A (zh) * | 2001-12-07 | 2002-06-26 | 清华大学 | 利用固相载体从生物粒子中分离核酸的方法 |
Family Cites Families (21)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
DE2513608C2 (de) * | 1975-03-27 | 1982-08-05 | Degussa Ag, 6000 Frankfurt | Verfahren zur Hydrophobierung von Kieselsäuren und Silikaten mit Organosilanen |
JPH03220158A (ja) | 1990-01-23 | 1991-09-27 | Sumitomo Pharmaceut Co Ltd | 光学活性カルバサイクリン誘導体の製造方法 |
US5726026A (en) | 1992-05-01 | 1998-03-10 | Trustees Of The University Of Pennsylvania | Mesoscale sample preparation device and systems for determination and processing of analytes |
US5587128A (en) * | 1992-05-01 | 1996-12-24 | The Trustees Of The University Of Pennsylvania | Mesoscale polynucleotide amplification devices |
JPH0824600A (ja) * | 1994-07-11 | 1996-01-30 | Tokuyama Corp | ガラス多孔質膜 |
US5641400A (en) * | 1994-10-19 | 1997-06-24 | Hewlett-Packard Company | Use of temperature control devices in miniaturized planar column devices and miniaturized total analysis systems |
JP3220158B2 (ja) | 1994-11-14 | 2001-10-22 | トラスティーズ・オブ・ザ・ユニバーシティ・オブ・ペンシルベニア | 分析対象物質の確定及び処理のためのメソ規模の試料前処理装置及びシステム |
KR100306951B1 (ko) * | 1995-12-05 | 2001-11-15 | 테칸 보스턴, 인코포레이티드 | 내장된정보과학에의해미세유체공학시스템내의유체유동을구동시키기위해구심가속도를이용하는장치및방법 |
US6193866B1 (en) * | 1996-03-27 | 2001-02-27 | Curagen Corporation | Separation of charged particles by a spatially and temporally varying electric field |
US5900481A (en) * | 1996-11-06 | 1999-05-04 | Sequenom, Inc. | Bead linkers for immobilizing nucleic acids to solid supports |
US6270970B1 (en) * | 1999-05-14 | 2001-08-07 | Promega Corporation | Mixed-bed solid phase and its use in the isolation of nucleic acids |
US6272939B1 (en) * | 1999-10-15 | 2001-08-14 | Applera Corporation | System and method for filling a substrate with a liquid sample |
WO2001032930A1 (en) | 1999-11-04 | 2001-05-10 | California Institute Of Technology | Methods and apparatuses for analyzing polynucleotide sequences |
CA2290731A1 (en) | 1999-11-26 | 2001-05-26 | D. Jed Harrison | Apparatus and method for trapping bead based reagents within microfluidic analysis system |
SE0001790D0 (sv) * | 2000-05-12 | 2000-05-12 | Aamic Ab | Hydrophobic barrier |
EP2299256A3 (en) * | 2000-09-15 | 2012-10-10 | California Institute Of Technology | Microfabricated crossflow devices and methods |
US6743516B2 (en) * | 2000-09-29 | 2004-06-01 | Guardian Industries Corporation | Highly durable hydrophobic coatings and methods |
US7378280B2 (en) * | 2000-11-16 | 2008-05-27 | California Institute Of Technology | Apparatus and methods for conducting assays and high throughput screening |
SE0004350D0 (sv) | 2000-11-27 | 2000-11-27 | Patrick Griss | System och metod för tillförlitliga passiva ventiler och gas volym dosering |
US7060171B1 (en) * | 2001-07-31 | 2006-06-13 | Caliper Life Sciences, Inc. | Methods and systems for reducing background signal in assays |
US20040011650A1 (en) * | 2002-07-22 | 2004-01-22 | Frederic Zenhausern | Method and apparatus for manipulating polarizable analytes via dielectrophoresis |
-
2004
- 2004-03-24 EP EP04722945.5A patent/EP1607748B1/en not_active Expired - Fee Related
- 2004-03-24 US US10/550,302 patent/US8012680B2/en not_active Expired - Fee Related
- 2004-03-24 JP JP2005504072A patent/JP5162095B2/ja not_active Expired - Fee Related
- 2004-03-24 CN CN2004800082843A patent/CN1764842B/zh not_active Expired - Fee Related
- 2004-03-24 WO PCT/JP2004/003998 patent/WO2004086055A1/ja active Application Filing
- 2004-03-24 KR KR1020057017815A patent/KR20050111785A/ko not_active Application Discontinuation
Patent Citations (2)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US6319469B1 (en) * | 1995-12-18 | 2001-11-20 | Silicon Valley Bank | Devices and methods for using centripetal acceleration to drive fluid movement in a microfluidics system |
CN1355319A (zh) * | 2001-12-07 | 2002-06-26 | 清华大学 | 利用固相载体从生物粒子中分离核酸的方法 |
Non-Patent Citations (7)
Title |
---|
Darwin R. Reyes, Dimitri lossifidis, Pierre-Alain Auroux,Andreas Manz.Micro total anlysis systems. 1. Introduction, theory, andtechnology.Analytical Chemistry74 12.2002,74(12),2629. |
Darwin R. Reyes, Dimitri lossifidis, Pierre-Alain Auroux,Andreas Manz.Micro total anlysis systems. 1. Introduction, theory, andtechnology.Analytical Chemistry74 12.2002,74(12),2629. * |
Kelley A. Wolfe, Michael C. Breadmore, James P. Landers,.Toward a microchip-based solid-phase extraction method forisolation of nucleic acids.Electrophoresis23.2002,23727-733. * |
Kiichi Sato, Manabu Tokeshi, Hiroko Kimura, TakehikoKitamori.Dtermination of carcinoembryonic antigen in human sera byintegrated bead-bed immunoasay in a microchip for cancerdiagnosis.Analytical Chemistry73 6.2001,73(6),1213-1218. |
Kiichi Sato, Manabu Tokeshi, Hiroko Kimura, TakehikoKitamori.Dtermination of carcinoembryonic antigen in human sera byintegrated bead-bed immunoasay in a microchip for cancerdiagnosis.Analytical Chemistry73 6.2001,73(6),1213-1218. * |
Kiichi Sato, Manabu Tokeshi, Tamao Odake, Hiroko Kimura,Takehiko Kitamori.Integration of an immunosorbent assay system: analysis ofsecretory human immunogllbulin A on polystyrene beads in amicrochip.Analytical Chemistry72 6.2000,72(6),1144-1147. |
Kiichi Sato, Manabu Tokeshi, Tamao Odake, Hiroko Kimura,Takehiko Kitamori.Integration of an immunosorbent assay system: analysis ofsecretory human immunogllbulin A on polystyrene beads in amicrochip.Analytical Chemistry72 6.2000,72(6),1144-1147. * |
Also Published As
Publication number | Publication date |
---|---|
EP1607748A1 (en) | 2005-12-21 |
US8012680B2 (en) | 2011-09-06 |
JPWO2004086055A1 (ja) | 2006-06-29 |
KR20050111785A (ko) | 2005-11-28 |
EP1607748A4 (en) | 2012-05-30 |
CN1764842A (zh) | 2006-04-26 |
EP1607748B1 (en) | 2013-05-15 |
WO2004086055A1 (ja) | 2004-10-07 |
US20060234241A1 (en) | 2006-10-19 |
JP5162095B2 (ja) | 2013-03-13 |
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Effective date of registration: 20151105 Address after: Tokyo, Japan, Japan Patentee after: Sony Corp Address before: Tokyo, Japan, Japan Patentee before: Sony Corporation Patentee before: Kanagawa Academy of Science and Technology |
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