CN1411872A - Microbial deodorant and its preparation method - Google Patents

Abstract

The present invention provides a microbial deodorant and its preparation method. It is a mixed fermentation liquor of torula yeast containing candida and small elliptic yeast containing saccharomyces, and the total microzyme amount of said two yeasts contained in its product is not less than 1X10 to the power 7/ml. Its preparation process is characterized by that it uses the above-mentioned two yeast as strain, and adopts seed liquor cultivation and fermentation cultivation processes, then the proper quantity of essence can be added in the fermented liquor so as to obtain the invented deodorant.

Description

A kind of microbial deodorant and preparation method thereof

The present invention relates to a kind of microbial deodorant and preparation method thereof.

Along with the develop rapidly of society, stench has become a kind of serious public hazards.Especially to house refuse and feces Application and Development, when carrying out resource conversion, the stench problem must be solved.The existing deodorizer of existing market mainly contains chemical deodorizing agent or plant deodorant.Plant deodorant extraction process complexity, production cost is higher; The chemical deodorizing agent also may cause secondary pollution except that cost is higher.

It is simple to the purpose of this invention is to provide a kind of manufacture method, and cost is low, and deodorizing effect is remarkable, is difficult for microbial deodorant that causes secondary pollution and preparation method thereof.

Microbial deodorant of the present invention is a kind of product protein yeast (pseudohypha that contains mycocandida (Candida), bacterium colony is smooth, the edge is smooth, can utilize glucose, sucrose, 1/3 Raffinose) and little ellipse garden yeast (the cell circle of Saccharomyces (Saccharomyces), can glucose fermentation, sucrose, galactose and whole Raffinoses) mixed culture fermentation liquid, the total bacteria count of contained two primary yeasts is no less than 1 * 10 in its product ⁷Individual/the ml order of magnitude.Said this 2 primary yeast all belongs to existing yeast kind, sees " fungus identification handbook " (Shanghai science tech publishing house, front page in 1979).

Can in this deodorizer, add the essence of 0.01～0.05% (V/V), with further raising deodorizing effect.

The preparation method of microbial deodorant of the present invention is characterized in that with the product protein yeast of mycocandida (Candida) and Saccharomyces (Saccharom yces) and little saccharomyces ellipsoideus be strain, carries out fermentation culture according to the following steps:

(1) respectively shaking table in the product protein yeast of mycocandida (Candida) and Saccharomyces (Saccharom yces) and the little saccharomyces ellipsoideus two strain liquid medium withins is cultivated, reached 1 * 10 at least to the bacterium number ⁸Individual/the ml order of magnitude, make seed liquor, said fluid medium is regulated pH value to 6.0～7.0 with sulphuric acid, through sterilization treatment for containing the aqueous solution of saccharide 0.5～5%, triammonium phosphate 0.2% (percentage by weight);

(2) in fermentation tank, be fermentation mother liquor with (1) described fluid medium, mix the seed liquor that adds the prepared two kinds of bacterium of previous step, the total amount of two kinds of seed liquor of inoculation is 1～5% (V/V) of fermentation mother liquor, fermentation culture to bacterium number reaches 1 * 10 at least ⁷Individual/the ml order of magnitude;

(3) essence that adds 0.01～0.05% (V/V) in the fermentation liquid of gained is promptly made this microbial deodorant.

This microbial deodorant at normal temperatures, effect duration can reach more than three months.

All there are many smelly antibacterials that causes in house refuse and the feces, after they decompose the organic principle in house refuse and the feces, just discharge odorant.The present invention is a kind of fermented product of microorganism, and it is acid that pH is, in the sprinkling process to airborne NH ₃Tool Absorption, fermentation liquid also can intercept distributing of smelly source stink, therefore can reach excellent deodorization effect immediately.Because the present invention also contains the multiple bioactie agent that deodorizing microorganism produces in fermentation production process, can effectively suppress to cause the physiologically active of smelly microorganism, and can be, thereby solve the smelly problem of house refuse and feces more at all with the nutrient substance of odor causing substance as self.Prepare described microbial deodorant with process of the present invention, have the technological process weak point, the advantage that cost is low.

Embodiment one

Add the 5g glucose in 500ml water, the 1g triammonium phosphate is regulated pH to 6.5 with sulphuric acid, is distributed into 4 bottles, sterilizes 15 minutes down for 105 ℃.Inoculate 2 primary yeasts respectively, 2 bottles of each bacterial classification inoculations.Grow to 1 * 10 for the condition of, rotating speed 150rpm down to bacterium in temperature ⁸Can take off seed liquor from shaking table behind individual/ml order of magnitude.Add 1 50g glucose in 15L water, the 30g triammonium phosphate is regulated pH to 6.5 with sulphuric acid, sterilizes 15 minutes down for 105 ℃ in fermentation tank.When treating that culture-liquid temp is reduced to 30 ℃, 4 bottles of seed liquor combined inoculations to fermentation tank, at 30 ℃ of temperature, tank pressure 0.05Mpa, ventilation 0.5L/H, are fermented under the condition of rotating speed 150rpm, got sample one time in per 4 hours, microscopically is observed counting.When the bacterium number reaches 1 * 10 ⁷Individual/as to stop fermentation during ml.In fermentation liquid, add 1.7ml essence at last, promptly make this microbial deodorant.

Embodiment two

Add the 4g glucose in 400ml water, the 0.8g triammonium phosphate is regulated pH to 6.0 with sulphuric acid, is distributed into 4 bottles, sterilizes 15 minutes down for 105 ℃.Inoculate 2 primary yeasts respectively, 2 bottles of each bacterial classification inoculations.Grow to 1 * 10 for the condition of, rotating speed 150rpm down to bacterium in temperature ⁸Can take off seed liquor from shaking table behind individual/ml order of magnitude.Add the 75g glucose in 15L water, the 30g triammonium phosphate was sterilized 15 minutes down for 105 ℃ in fermentation tank.When treating that culture-liquid temp is reduced to 30 ℃, 4 bottles of seed liquor combined inoculations to fermentation tank, at 30 ℃ of temperature, tank pressure 0.05Mpa, ventilation 0.5L/H, are fermented under the condition of rotating speed 150rpm, got sample one time in per 4 hours, microscopically is observed counting.When the bacterium number reaches 1 * 10 ⁷Individual/as to stop fermentation during ml.In fermentation liquid, add 1.7ml essence at last, promptly make this microbial deodorant.

Embodiment three

Add 20g sucrose in 500ml water, the 1g triammonium phosphate is regulated pH to 7.0 with sulphuric acid, is distributed into 4 bottles, sterilizes 15 minutes down for 105 ℃.Inoculate 2 primary yeasts respectively, 2 bottles of each bacterial classification inoculations.Grow to 1 * 10 for the condition of, rotating speed 150rpm down to bacterium in temperature ⁸Can take off seed liquor from shaking table behind individual/ml order of magnitude.Add the 750g glucose in 15L water, the 30g triammonium phosphate is regulated pH to 7.0 with sulphuric acid, sterilizes 15 minutes down for 105 ℃ in fermentation tank.When treating that culture-liquid temp is reduced to 30 ℃, 4 bottles of seed liquor combined inoculations to fermentation tank, at 30 ℃ of temperature, tank pressure 0.05Mpa, ventilation 0.5L/H, are fermented under the condition of rotating speed 150rpm, got sample one time in per 4 hours, microscopically is observed counting.When the bacterium number reaches 1 * 10 ⁷Individual/as to stop fermentation during ml.In fermentation liquid, add 8.5ml essence at last, promptly make this microbial deodorant.

Claims (3)

1. a microbial deodorant is a kind of microbial fermentation solution, it is characterized in that wherein containing product protein yeast and the Saccharomyces (Saccharom yces) and the little saccharomyces ellipsoideus of mycocandida (Candida), and its total bacteria count is no less than 1 * 10 ⁷Individual/the ml order of magnitude.

2. microbial deodorant according to claim 1 is characterized in that also being added with in this deodorizer the essence of 0.01～0.05% (V/V).

3. the preparation method of a microbial deodorant is characterized in that with the product protein yeast of mycocandida (Candida) and Saccharomyces (Saccharom yces) and little saccharomyces ellipsoideus be strain, carries out fermentation culture according to the following steps:

(1) respectively shaking table in the product protein yeast of mycocandida (Candida) and Saccharomyces (Saccharom yces) and the little saccharomyces ellipsoideus two strain liquid medium withins is cultivated, reached 1 * 10 at least to the bacterium number ⁸Individual/the ml order of magnitude, make seed liquor, said fluid medium is regulated pH value to 6.0～7.0 with sulphuric acid, through sterilization treatment for containing the aqueous solution of saccharide 0.5～5%, triammonium phosphate 0.2% (percentage by weight);

(2) in fermentation tank, be fermentation mother liquor with (1) described fluid medium, mix the seed liquor that adds the prepared two kinds of bacterium of previous step, the total amount of two kinds of seed liquor of inoculation is 1～5% (V/V) of fermentation mother liquor, fermentation culture to bacterium number reaches 1 * 10 at least ⁷Individual/the ml order of magnitude;

(3) in the fermentation liquid of gained, add the essence of 0.01～0.05% (V/V).