CN1292824A - 海藻糖生物合成基因在植物中的表达 - Google Patents

海藻糖生物合成基因在植物中的表达 Download PDF

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Publication number
CN1292824A
CN1292824A CN998037532A CN99803753A CN1292824A CN 1292824 A CN1292824 A CN 1292824A CN 998037532 A CN998037532 A CN 998037532A CN 99803753 A CN99803753 A CN 99803753A CN 1292824 A CN1292824 A CN 1292824A
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plant
promoter
gene
trehalose
nucleotide sequence
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E·G·利贝尔
P·B·海菲兹
S·A·高夫
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Syngenta Participations AG
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Novartis AG
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CN998037532A 1998-03-11 1999-03-09 海藻糖生物合成基因在植物中的表达 Pending CN1292824A (zh)

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US7766598P 1998-03-11 1998-03-11
US60/077,665 1998-03-11

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JP (1) JP2002505875A (enExample)
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AU (1) AU748604B2 (enExample)
BR (1) BR9909258A (enExample)
CA (1) CA2320896A1 (enExample)
EA (1) EA200000926A1 (enExample)
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Cited By (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN100355883C (zh) * 2004-12-10 2007-12-19 中国科学院上海生命科学研究院 水稻抗逆相关基因-海藻糖-6-磷酸磷酸化酶基因及其应用
CN101289514B (zh) * 2008-06-13 2011-01-19 北京北方杰士生物科技有限责任公司 一种培育耐逆植物的方法及其专用dna片段
CN103975057A (zh) * 2011-09-13 2014-08-06 美国世多乐集团公司 通过施用海藻糖提高作物产量的方法
CN106801102A (zh) * 2017-02-23 2017-06-06 浙江省检验检疫科学技术研究院 苋属的实时荧光pcr鉴定方法
CN108624598A (zh) * 2018-06-06 2018-10-09 福建农林大学 Tppf基因在调控植物海藻糖合成和提高植物抗旱性中的应用
CN110582567A (zh) * 2017-05-04 2019-12-17 嘉吉公司 经遗传修饰的表达海藻糖酶的酵母及使用此类经遗传修饰的酵母的发酵方法

Families Citing this family (27)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US7214858B2 (en) * 1999-03-31 2007-05-08 E. I. Du Pont De Nemours And Company Plant genes encoding trehalose metabolism enzymes
WO2001007590A2 (en) * 1999-07-27 2001-02-01 Syngenta Participations Ag Chimeric genes for plastid expression
CA2641218C (en) 1999-12-16 2014-02-25 Monsanto Technology Llc Hybrid promoter for use in plants
US20040064846A1 (en) * 2001-02-28 2004-04-01 Henry Daniell Genetic engineering of drought tolerance via a plastid genome
CA2401855A1 (en) * 2000-02-29 2001-09-07 Auburn University Genetic engineering of drought tolerance via a plastid genome
WO2003091413A2 (en) * 2002-04-23 2003-11-06 The Scripps Research Institute Expression of polypeptides in chloroplasts, and compositions and methods for expressing same
EP1375669A1 (en) * 2002-06-13 2004-01-02 Stichting Voor De Technische Wetenschappen Method for enhancing the disease resistance in plants by altering trehalose-6-phosphate levels
KR100440725B1 (ko) * 2002-06-20 2004-07-15 주식회사 그린진 바이오텍 비생물성 스트레스에 대한 단자엽 식물의 내성을증가시키는 방법
JP2006505279A (ja) * 2002-11-06 2006-02-16 コーネル リサーチ ファンデーション, インコーポレーティッド Tps植物遺伝子構築体及び形質転換体
WO2004044148A2 (en) * 2002-11-08 2004-05-27 Paradigm Genetics, Inc. Methods for the identification of trehalose-6-phosphate synthase as antibiotics
EP2366790A1 (en) * 2004-04-20 2011-09-21 Syngenta Participations AG Regulatory sequences for expressing gene products in plant reproductive tissue
CA2588372A1 (en) * 2004-12-03 2006-06-08 Syngenta Participations Ag Stress tolerance in plants through selective inhibition of trehalose-6-phosphate phosphatase
CA2592704C (en) 2005-01-07 2016-05-24 E. I. Du Pont De Nemours And Company Nucleotide sequences encoding ramosa3 and sister of ramosa3 and methods of use for same
CA2621536C (en) 2005-09-06 2013-12-17 Plant Research International B.V. A transgenic plant having enhanced drought tolerance
AU2008237350B2 (en) * 2007-04-03 2013-02-07 Tabletop Energy Methods for transforming eukaryotic algae
CN101835901B (zh) * 2007-06-01 2013-03-06 蓝宝石能源公司 遗传修饰的光合生物的高通量筛选
US9506075B2 (en) * 2007-08-01 2016-11-29 Bioglow, Llc Bioluminescent plants comprising bacterial LUX operon and methods of making same
EP2203542A1 (en) * 2007-09-11 2010-07-07 Sapphire Energy, Inc. Methods of producing organic products with photosynthetic organisms and products and compositions thereof
NZ598302A (en) * 2007-09-11 2013-08-30 Sapphire Energy Inc Molecule production by photosynthetic organisms
EP2255003B1 (en) 2008-02-15 2015-01-28 Ceres Inc. Drought and heat tolerance in plants
WO2010061404A1 (en) * 2008-11-27 2010-06-03 International Centre For Genetic Engineering And Biotechnology A method of expressing foreign protein in plastids
EP3056570A1 (en) * 2009-10-22 2016-08-17 BASF Plant Science Company GmbH Plants having enhanced-yield-related traits and a method for making the same
CN103998611A (zh) 2011-11-03 2014-08-20 先正达参股股份有限公司 用于增强植物中光同化作用的多核苷酸、多肽和方法
GB201609969D0 (en) 2016-06-07 2016-07-20 Univ Cape Town Drought resistance multigene construct
US11242536B2 (en) * 2017-09-26 2022-02-08 Rutgers, The State University Of New Jersey Quality protein maize based on increased sulfur reduction in leaf cells
CN108728457B (zh) * 2018-06-22 2021-09-17 山东省药学科学院 一种海藻糖合成酶基因优化序列及其应用
CN119744875A (zh) * 2024-12-26 2025-04-04 西华师范大学 苯并噻二唑在增强半夏光合系统稳定性和提高半夏耐旱性中的应用

Family Cites Families (13)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5614395A (en) * 1988-03-08 1997-03-25 Ciba-Geigy Corporation Chemically regulatable and anti-pathogenic DNA sequences and uses thereof
US5693507A (en) * 1988-09-26 1997-12-02 Auburn University Genetic engineering of plant chloroplasts
US5792921A (en) * 1992-02-14 1998-08-11 Londesborough; John Increasing the trehalose content of organisms by transforming them with combinations of the structural genes for trehalose synthase
FI943133A0 (fi) * 1994-06-29 1994-06-29 Alko Ab Oy Transgena vaexter
US5422254A (en) 1992-02-14 1995-06-06 Oy Alko Ab Method to increase the trehalose content of organisms by transforming them with the structural genes for the short and long chains of yeast trehalose synthase
CN1131315C (zh) 1993-06-30 2003-12-17 辛根塔莫根有限公司 植物中海藻糖的产生
US5780709A (en) * 1993-08-25 1998-07-14 Dekalb Genetics Corporation Transgenic maize with increased mannitol content
WO1995014098A1 (en) * 1993-11-19 1995-05-26 Biotechnology Research And Development Corporation Chimeric regulatory regions and gene cassettes for expression of genes in plants
US5545818A (en) * 1994-03-11 1996-08-13 Calgene Inc. Expression of Bacillus thuringiensis cry proteins in plant plastids
US5925806A (en) * 1995-06-06 1999-07-20 Mcbride; Kevin E. Controlled expression of transgenic constructs in plant plastids
MX205414B (es) * 1996-05-08 2001-12-07 Univ Mexico Nacional Autonoma Metodo para incrementar de trehalosa de los organismos por medio de su transformacion con el adnc de la trehalosa-6-fosfato sintasa/fosfatasa de selaginella lepidophylla.
JPH11513897A (ja) * 1996-07-23 1999-11-30 ノバルティス アクチェンゲゼルシャフト 化学的誘発性アラビドプシスpr−1プロモーター
ZA989886B (en) * 1997-10-30 1999-04-30 Mogen Int Nuclear male sterile plants method of producing same and methods to restore fertility

Cited By (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN100355883C (zh) * 2004-12-10 2007-12-19 中国科学院上海生命科学研究院 水稻抗逆相关基因-海藻糖-6-磷酸磷酸化酶基因及其应用
CN101289514B (zh) * 2008-06-13 2011-01-19 北京北方杰士生物科技有限责任公司 一种培育耐逆植物的方法及其专用dna片段
CN103975057A (zh) * 2011-09-13 2014-08-06 美国世多乐集团公司 通过施用海藻糖提高作物产量的方法
CN106801102A (zh) * 2017-02-23 2017-06-06 浙江省检验检疫科学技术研究院 苋属的实时荧光pcr鉴定方法
CN106801102B (zh) * 2017-02-23 2020-11-20 浙江省检验检疫科学技术研究院 苋属的实时荧光pcr鉴定方法
CN110582567A (zh) * 2017-05-04 2019-12-17 嘉吉公司 经遗传修饰的表达海藻糖酶的酵母及使用此类经遗传修饰的酵母的发酵方法
CN110582567B (zh) * 2017-05-04 2024-03-08 嘉吉公司 经遗传修饰的表达海藻糖酶的酵母及使用此类经遗传修饰的酵母的发酵方法
CN108624598A (zh) * 2018-06-06 2018-10-09 福建农林大学 Tppf基因在调控植物海藻糖合成和提高植物抗旱性中的应用

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JP2002505875A (ja) 2002-02-26
AU748604B2 (en) 2002-06-06
KR20010082520A (ko) 2001-08-30
US20030009784A1 (en) 2003-01-09
IL138191A0 (en) 2001-10-31
WO1999046370A3 (en) 1999-11-18
EP1064365A2 (en) 2001-01-03
HUP0102598A3 (en) 2003-03-28
HUP0102598A2 (hu) 2001-11-28
US7030294B2 (en) 2006-04-18
WO1999046370A2 (en) 1999-09-16
EA200000926A1 (ru) 2001-06-25
BR9909258A (pt) 2000-11-28
US20040078848A1 (en) 2004-04-22
US6686516B2 (en) 2004-02-03
CA2320896A1 (en) 1999-09-16
TR200002624T2 (tr) 2000-11-21
PL343460A1 (en) 2001-08-13
IN2000CN00368A (enExample) 2010-03-19

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