KR20010082520A - 식물에서 트레할로즈 생합성 유전자의 발현 - Google Patents

식물에서 트레할로즈 생합성 유전자의 발현 Download PDF

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Publication number
KR20010082520A
KR20010082520A KR1020007010115A KR20007010115A KR20010082520A KR 20010082520 A KR20010082520 A KR 20010082520A KR 1020007010115 A KR1020007010115 A KR 1020007010115A KR 20007010115 A KR20007010115 A KR 20007010115A KR 20010082520 A KR20010082520 A KR 20010082520A
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South Korea
Prior art keywords
plant
promoter
trehalose
gene
nucleotide sequence
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KR1020007010115A
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Korean (ko)
Inventor
레벨에도우아르트귈라우메
헤이페츠피터버나드
고프스테펜아서
Original Assignee
한스 루돌프 하우스, 헨리테 브룬너, 베아트리체 귄터
노파르티스 아게
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Publication of KR20010082520A publication Critical patent/KR20010082520A/ko
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    • C—CHEMISTRY; METALLURGY
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    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/1048—Glycosyltransferases (2.4)
    • C12N9/1051—Hexosyltransferases (2.4.1)
    • C—CHEMISTRY; METALLURGY
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    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8201—Methods for introducing genetic material into plant cells, e.g. DNA, RNA, stable or transient incorporation, tissue culture methods adapted for transformation
    • C12N15/8214—Plastid transformation
    • C—CHEMISTRY; METALLURGY
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    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8216—Methods for controlling, regulating or enhancing expression of transgenes in plant cells
    • C—CHEMISTRY; METALLURGY
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    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8216—Methods for controlling, regulating or enhancing expression of transgenes in plant cells
    • C12N15/8217—Gene switch
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8216—Methods for controlling, regulating or enhancing expression of transgenes in plant cells
    • C12N15/8222—Developmentally regulated expression systems, tissue, organ specific, temporal or spatial regulation
    • C—CHEMISTRY; METALLURGY
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    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8216—Methods for controlling, regulating or enhancing expression of transgenes in plant cells
    • C12N15/8222—Developmentally regulated expression systems, tissue, organ specific, temporal or spatial regulation
    • C12N15/8223—Vegetative tissue-specific promoters
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    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8216—Methods for controlling, regulating or enhancing expression of transgenes in plant cells
    • C12N15/8237—Externally regulated expression systems
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8242—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits
    • C12N15/8243—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits involving biosynthetic or metabolic pathways, i.e. metabolic engineering, e.g. nicotine, caffeine
    • C12N15/8245—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits involving biosynthetic or metabolic pathways, i.e. metabolic engineering, e.g. nicotine, caffeine involving modified carbohydrate or sugar alcohol metabolism, e.g. starch biosynthesis
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    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8261—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield
    • C12N15/8271—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance
    • C12N15/8273—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance for drought, cold, salt resistance

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  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Biotechnology (AREA)
  • Chemical & Material Sciences (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Organic Chemistry (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • General Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Microbiology (AREA)
  • Cell Biology (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Plant Pathology (AREA)
  • Nutrition Science (AREA)
  • Medicinal Chemistry (AREA)
  • Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Saccharide Compounds (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
KR1020007010115A 1998-03-11 1999-03-09 식물에서 트레할로즈 생합성 유전자의 발현 Withdrawn KR20010082520A (ko)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US7766598P 1998-03-11 1998-03-11
US60/077,665 1998-03-11

Publications (1)

Publication Number Publication Date
KR20010082520A true KR20010082520A (ko) 2001-08-30

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KR1020007010115A Withdrawn KR20010082520A (ko) 1998-03-11 1999-03-09 식물에서 트레할로즈 생합성 유전자의 발현

Country Status (15)

Country Link
US (2) US6686516B2 (enExample)
EP (1) EP1064365A2 (enExample)
JP (1) JP2002505875A (enExample)
KR (1) KR20010082520A (enExample)
CN (1) CN1292824A (enExample)
AU (1) AU748604B2 (enExample)
BR (1) BR9909258A (enExample)
CA (1) CA2320896A1 (enExample)
EA (1) EA200000926A1 (enExample)
HU (1) HUP0102598A3 (enExample)
IL (1) IL138191A0 (enExample)
IN (1) IN2000CN00368A (enExample)
PL (1) PL343460A1 (enExample)
TR (1) TR200002624T2 (enExample)
WO (1) WO1999046370A2 (enExample)

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WO2001007590A2 (en) * 1999-07-27 2001-02-01 Syngenta Participations Ag Chimeric genes for plastid expression
EP2339005A3 (en) 1999-12-16 2011-10-12 Monsanto Technology LLC Dna constructs for expression of heterologous polypeptides in plants
US20040064846A1 (en) * 2001-02-28 2004-04-01 Henry Daniell Genetic engineering of drought tolerance via a plastid genome
CA2401855A1 (en) * 2000-02-29 2001-09-07 Auburn University Genetic engineering of drought tolerance via a plastid genome
EP1576101A4 (en) * 2002-04-23 2007-10-31 Scripps Research Inst EXPRESSION OF POLYPEPTIDES IN CHLOROPLASTS AND COMPOSITIONS AND METHODS OF EXPRESSION THEREOF
EP1375669A1 (en) * 2002-06-13 2004-01-02 Stichting Voor De Technische Wetenschappen Method for enhancing the disease resistance in plants by altering trehalose-6-phosphate levels
KR100440725B1 (ko) * 2002-06-20 2004-07-15 주식회사 그린진 바이오텍 비생물성 스트레스에 대한 단자엽 식물의 내성을증가시키는 방법
EP1563060A4 (en) * 2002-11-06 2006-03-22 Cornell Res Foundation Inc TPS PLANT ENGINEERING AND TRANSFORMERS
AU2003290635A1 (en) * 2002-11-08 2004-06-03 Paradigm Genetics, Inc. Methods for the identification of trehalose-6-phosphate synthase as antibiotics
WO2005102034A2 (en) * 2004-04-20 2005-11-03 Syngenta Participations Ag Regulatory sequences for expressing gene products in plant reproductive tissue
CA2588372A1 (en) * 2004-12-03 2006-06-08 Syngenta Participations Ag Stress tolerance in plants through selective inhibition of trehalose-6-phosphate phosphatase
CN100355883C (zh) * 2004-12-10 2007-12-19 中国科学院上海生命科学研究院 水稻抗逆相关基因－海藻糖－6－磷酸磷酸化酶基因及其应用
WO2006074437A2 (en) * 2005-01-07 2006-07-13 E.I. Dupont De Nemours And Company Nucleotide sequences encoding ramosa3 and sister of ramosa3 and methods of use for same
DE602005027621D1 (de) 2005-09-06 2011-06-01 Stichting Dienst Landbouwkundi Verwendung einer Nukleinsäure zur Herstellung einer transgenen Pflanze mit verbesserter Toleranz gegenüber Trockenheit
US9284568B2 (en) * 2007-04-03 2016-03-15 Tabletop Energy Methods for transforming eukaryotic algae
DK2162537T3 (da) * 2007-06-01 2012-04-23 Sapphire Energy Inc Anvendelse af genetisk modificerede organismer til at frembringe biomasse-nedbrydende enzymer.
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EP2203542A1 (en) * 2007-09-11 2010-07-07 Sapphire Energy, Inc. Methods of producing organic products with photosynthetic organisms and products and compositions thereof
NZ598302A (en) * 2007-09-11 2013-08-30 Sapphire Energy Inc Molecule production by photosynthetic organisms
CA2713208C (en) 2008-02-15 2021-02-16 Ceres, Inc. Drought and heat tolerance in plants
CN101289514B (zh) * 2008-06-13 2011-01-19 北京北方杰士生物科技有限责任公司 一种培育耐逆植物的方法及其专用dna片段
WO2010061404A1 (en) * 2008-11-27 2010-06-03 International Centre For Genetic Engineering And Biotechnology A method of expressing foreign protein in plastids
WO2011048009A1 (en) * 2009-10-22 2011-04-28 Basf Plant Science Company Gmbh Plants having enhanced yield-related traits and a method for making the same
KR20140067124A (ko) * 2011-09-13 2014-06-03 스톨러 엔터프라이지즈, 인크. 트레할로스의 적용에 의한 작물 수량의 향상 방법
HUP1400505A2 (en) 2011-11-03 2015-03-02 Syngenta Participations Ag Polynucleotides, polypeptides and methods for enhancing photossimilation in plants
GB201609969D0 (en) 2016-06-07 2016-07-20 Univ Cape Town Drought resistance multigene construct
CN106801102B (zh) * 2017-02-23 2020-11-20 浙江省检验检疫科学技术研究院 苋属的实时荧光pcr鉴定方法
CA3062324A1 (en) * 2017-05-04 2018-11-08 Cargill, Incorporated Genetically modified trehalase-expressing yeasts and fermentation processes using such genetically modified yeasts
WO2019067571A1 (en) * 2017-09-26 2019-04-04 Rutgers, The State University University Of New Jersey QUALITY PROTEIN CORN BASED ON INCREASED REDUCTION OF SULFUR IN SHEET CELLS
CN108624598A (zh) * 2018-06-06 2018-10-09 福建农林大学 Tppf基因在调控植物海藻糖合成和提高植物抗旱性中的应用
CN108728457B (zh) * 2018-06-22 2021-09-17 山东省药学科学院 一种海藻糖合成酶基因优化序列及其应用
CN119744875A (zh) * 2024-12-26 2025-04-04 西华师范大学 苯并噻二唑在增强半夏光合系统稳定性和提高半夏耐旱性中的应用

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MX205414B (es) * 1996-05-08 2001-12-07 Univ Mexico Nacional Autonoma Metodo para incrementar de trehalosa de los organismos por medio de su transformacion con el adnc de la trehalosa-6-fosfato sintasa/fosfatasa de selaginella lepidophylla.
AU708850B2 (en) * 1996-07-23 1999-08-12 Novartis Ag Chemically-inducible arabidopsis PR-1 promoter
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US20030009784A1 (en) 2003-01-09
US6686516B2 (en) 2004-02-03
TR200002624T2 (tr) 2000-11-21
HUP0102598A3 (en) 2003-03-28
WO1999046370A2 (en) 1999-09-16
IN2000CN00368A (enExample) 2010-03-19
US7030294B2 (en) 2006-04-18
CA2320896A1 (en) 1999-09-16
BR9909258A (pt) 2000-11-28
IL138191A0 (en) 2001-10-31
AU3329599A (en) 1999-09-27
EP1064365A2 (en) 2001-01-03
WO1999046370A3 (en) 1999-11-18
HUP0102598A2 (hu) 2001-11-28
PL343460A1 (en) 2001-08-13
CN1292824A (zh) 2001-04-25
AU748604B2 (en) 2002-06-06
US20040078848A1 (en) 2004-04-22
JP2002505875A (ja) 2002-02-26
EA200000926A1 (ru) 2001-06-25

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