CN1426465A - 能从较大的核苷酸序列中以闭合环状形式释放,并且允许位点特异性地表达和/或发育调控性地表达所选遗传序列的构建体 - Google Patents
能从较大的核苷酸序列中以闭合环状形式释放,并且允许位点特异性地表达和/或发育调控性地表达所选遗传序列的构建体 Download PDFInfo
- Publication number
- CN1426465A CN1426465A CN01808621A CN01808621A CN1426465A CN 1426465 A CN1426465 A CN 1426465A CN 01808621 A CN01808621 A CN 01808621A CN 01808621 A CN01808621 A CN 01808621A CN 1426465 A CN1426465 A CN 1426465A
- Authority
- CN
- China
- Prior art keywords
- sequence
- construct
- rep
- cell
- mentioned
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
- 230000002068 genetic effect Effects 0.000 title claims abstract description 111
- 230000014509 gene expression Effects 0.000 title claims abstract description 107
- 239000002773 nucleotide Substances 0.000 title claims abstract description 98
- 125000003729 nucleotide group Chemical group 0.000 title claims abstract description 98
- 230000001105 regulatory effect Effects 0.000 title claims abstract description 30
- 108090000623 proteins and genes Proteins 0.000 claims abstract description 187
- 102000040430 polynucleotide Human genes 0.000 claims abstract description 102
- 108091033319 polynucleotide Proteins 0.000 claims abstract description 102
- 239000002157 polynucleotide Substances 0.000 claims abstract description 102
- 102000004169 proteins and genes Human genes 0.000 claims abstract description 69
- 210000003527 eukaryotic cell Anatomy 0.000 claims abstract description 22
- 230000001939 inductive effect Effects 0.000 claims abstract description 19
- 108091026890 Coding region Proteins 0.000 claims abstract description 16
- 230000035897 transcription Effects 0.000 claims abstract description 14
- 238000013518 transcription Methods 0.000 claims abstract description 14
- 230000030833 cell death Effects 0.000 claims abstract description 5
- 210000004027 cell Anatomy 0.000 claims description 152
- 241000196324 Embryophyta Species 0.000 claims description 98
- 108020004414 DNA Proteins 0.000 claims description 90
- 241000700605 Viruses Species 0.000 claims description 73
- 238000000034 method Methods 0.000 claims description 62
- 108090000765 processed proteins & peptides Proteins 0.000 claims description 59
- 108091028043 Nucleic acid sequence Proteins 0.000 claims description 48
- 230000000694 effects Effects 0.000 claims description 37
- 102000004196 processed proteins & peptides Human genes 0.000 claims description 36
- 229920001184 polypeptide Polymers 0.000 claims description 31
- 230000001276 controlling effect Effects 0.000 claims description 29
- 235000013311 vegetables Nutrition 0.000 claims description 25
- 108020004999 messenger RNA Proteins 0.000 claims description 18
- 210000001236 prokaryotic cell Anatomy 0.000 claims description 18
- 241001494793 Nanovirus Species 0.000 claims description 15
- 241001147420 ssDNA viruses Species 0.000 claims description 14
- 102000053602 DNA Human genes 0.000 claims description 13
- 240000008042 Zea mays Species 0.000 claims description 11
- 235000002017 Zea mays subsp mays Nutrition 0.000 claims description 11
- 241000702463 Geminiviridae Species 0.000 claims description 10
- 235000005824 Zea mays ssp. parviglumis Nutrition 0.000 claims description 10
- 235000005822 corn Nutrition 0.000 claims description 10
- 230000009261 transgenic effect Effects 0.000 claims description 10
- 238000003491 array Methods 0.000 claims description 9
- 230000005059 dormancy Effects 0.000 claims description 8
- 241000702286 Bean golden mosaic virus Species 0.000 claims description 7
- 230000015572 biosynthetic process Effects 0.000 claims description 7
- 230000009385 viral infection Effects 0.000 claims description 7
- 241000206602 Eukaryota Species 0.000 claims description 6
- 108700007698 Genetic Terminator Regions Proteins 0.000 claims description 6
- 230000008676 import Effects 0.000 claims description 6
- 230000005030 transcription termination Effects 0.000 claims description 6
- 230000000295 complement effect Effects 0.000 claims description 5
- 238000000926 separation method Methods 0.000 claims description 5
- 238000010008 shearing Methods 0.000 claims description 3
- 238000012239 gene modification Methods 0.000 claims 4
- 230000005017 genetic modification Effects 0.000 claims 4
- 235000013617 genetically modified food Nutrition 0.000 claims 4
- 101710150114 Protein rep Proteins 0.000 claims 2
- 101710152114 Replication protein Proteins 0.000 claims 2
- 108020004638 Circular DNA Proteins 0.000 claims 1
- 230000003612 virological effect Effects 0.000 abstract description 5
- 230000006907 apoptotic process Effects 0.000 abstract description 3
- 230000007246 mechanism Effects 0.000 abstract description 3
- 206010028980 Neoplasm Diseases 0.000 abstract description 2
- 201000011510 cancer Diseases 0.000 abstract description 2
- 230000001404 mediated effect Effects 0.000 abstract description 2
- 244000052769 pathogen Species 0.000 abstract description 2
- 206010021928 Infertility female Diseases 0.000 abstract 1
- 206010021929 Infertility male Diseases 0.000 abstract 1
- 208000007466 Male Infertility Diseases 0.000 abstract 1
- 239000013612 plasmid Substances 0.000 description 99
- 241000702292 Tobacco yellow dwarf virus Species 0.000 description 82
- 244000061176 Nicotiana tabacum Species 0.000 description 55
- 235000002637 Nicotiana tabacum Nutrition 0.000 description 55
- 235000018102 proteins Nutrition 0.000 description 46
- 210000001519 tissue Anatomy 0.000 description 41
- 108010016529 Bacillus amyloliquefaciens ribonuclease Proteins 0.000 description 40
- 239000012634 fragment Substances 0.000 description 29
- 241000725138 Banana bunchy top virus Species 0.000 description 28
- 238000006243 chemical reaction Methods 0.000 description 26
- 241000701489 Cauliflower mosaic virus Species 0.000 description 24
- 235000018290 Musa x paradisiaca Nutrition 0.000 description 22
- 244000291473 Musa acuminata Species 0.000 description 21
- 238000010586 diagram Methods 0.000 description 17
- 210000000056 organ Anatomy 0.000 description 17
- 241000589158 Agrobacterium Species 0.000 description 16
- 102000016938 Catalase Human genes 0.000 description 16
- 108010053835 Catalase Proteins 0.000 description 16
- 108091034117 Oligonucleotide Proteins 0.000 description 16
- 108700026244 Open Reading Frames Proteins 0.000 description 16
- 108700005078 Synthetic Genes Proteins 0.000 description 16
- 101100437498 Escherichia coli (strain K12) uidA gene Proteins 0.000 description 15
- 239000003795 chemical substances by application Substances 0.000 description 15
- 150000007523 nucleic acids Chemical class 0.000 description 15
- 102000039446 nucleic acids Human genes 0.000 description 14
- 108020004707 nucleic acids Proteins 0.000 description 14
- 230000001717 pathogenic effect Effects 0.000 description 14
- 239000013604 expression vector Substances 0.000 description 13
- 241000234295 Musa Species 0.000 description 12
- 108091092584 GDNA Proteins 0.000 description 11
- 241000723873 Tobacco mosaic virus Species 0.000 description 11
- 238000010367 cloning Methods 0.000 description 11
- 239000005090 green fluorescent protein Substances 0.000 description 11
- 208000015181 infectious disease Diseases 0.000 description 11
- 108091006905 Human Serum Albumin Proteins 0.000 description 10
- 108020004682 Single-Stranded DNA Proteins 0.000 description 10
- 101710123496 Spindolin Proteins 0.000 description 10
- 238000004458 analytical method Methods 0.000 description 10
- 239000003550 marker Substances 0.000 description 10
- 239000000523 sample Substances 0.000 description 10
- 108091032973 (ribonucleotides)n+m Proteins 0.000 description 9
- 102000004190 Enzymes Human genes 0.000 description 9
- 108090000790 Enzymes Proteins 0.000 description 9
- 102000008100 Human Serum Albumin Human genes 0.000 description 9
- 108091029795 Intergenic region Proteins 0.000 description 9
- 230000033228 biological regulation Effects 0.000 description 9
- 230000029087 digestion Effects 0.000 description 9
- 208000024891 symptom Diseases 0.000 description 9
- 241000894006 Bacteria Species 0.000 description 8
- 108700008625 Reporter Genes Proteins 0.000 description 8
- 239000000969 carrier Substances 0.000 description 8
- 238000001514 detection method Methods 0.000 description 8
- 238000011160 research Methods 0.000 description 8
- 230000001131 transforming effect Effects 0.000 description 8
- 241001465754 Metazoa Species 0.000 description 7
- 102100023050 Nuclear factor NF-kappa-B p105 subunit Human genes 0.000 description 7
- 208000027418 Wounds and injury Diseases 0.000 description 7
- 230000013020 embryo development Effects 0.000 description 7
- 238000001976 enzyme digestion Methods 0.000 description 7
- 239000011859 microparticle Substances 0.000 description 7
- 239000013598 vector Substances 0.000 description 7
- 235000003934 Abelmoschus esculentus Nutrition 0.000 description 6
- 241000589155 Agrobacterium tumefaciens Species 0.000 description 6
- ZHNUHDYFZUAESO-UHFFFAOYSA-N Formamide Chemical compound NC=O ZHNUHDYFZUAESO-UHFFFAOYSA-N 0.000 description 6
- 108091081024 Start codon Proteins 0.000 description 6
- 230000004913 activation Effects 0.000 description 6
- 230000000692 anti-sense effect Effects 0.000 description 6
- 239000002299 complementary DNA Substances 0.000 description 6
- 101150066583 rep gene Proteins 0.000 description 6
- 150000003839 salts Chemical class 0.000 description 6
- 239000000725 suspension Substances 0.000 description 6
- 238000013519 translation Methods 0.000 description 6
- 101150101900 uidA gene Proteins 0.000 description 6
- 241001124076 Aphididae Species 0.000 description 5
- 101710132601 Capsid protein Proteins 0.000 description 5
- 101710094648 Coat protein Proteins 0.000 description 5
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 description 5
- 241000233866 Fungi Species 0.000 description 5
- 101710125418 Major capsid protein Proteins 0.000 description 5
- 206010028851 Necrosis Diseases 0.000 description 5
- 101710141454 Nucleoprotein Proteins 0.000 description 5
- 101710083689 Probable capsid protein Proteins 0.000 description 5
- 241000723792 Tobacco etch virus Species 0.000 description 5
- 230000000735 allogeneic effect Effects 0.000 description 5
- 230000003321 amplification Effects 0.000 description 5
- 238000013459 approach Methods 0.000 description 5
- 239000002585 base Substances 0.000 description 5
- 230000008859 change Effects 0.000 description 5
- 238000005520 cutting process Methods 0.000 description 5
- 238000004520 electroporation Methods 0.000 description 5
- 230000001965 increasing effect Effects 0.000 description 5
- 230000017074 necrotic cell death Effects 0.000 description 5
- 238000003199 nucleic acid amplification method Methods 0.000 description 5
- 210000004940 nucleus Anatomy 0.000 description 5
- 230000008488 polyadenylation Effects 0.000 description 5
- 230000010076 replication Effects 0.000 description 5
- 238000012360 testing method Methods 0.000 description 5
- 230000014621 translational initiation Effects 0.000 description 5
- 238000011144 upstream manufacturing Methods 0.000 description 5
- 101710169849 Catalase isozyme A Proteins 0.000 description 4
- 101710169850 Catalase isozyme B Proteins 0.000 description 4
- 102100029091 Exportin-2 Human genes 0.000 description 4
- 101710147878 Exportin-2 Proteins 0.000 description 4
- 241000238631 Hexapoda Species 0.000 description 4
- 101100288095 Klebsiella pneumoniae neo gene Proteins 0.000 description 4
- 102000015636 Oligopeptides Human genes 0.000 description 4
- 108010038807 Oligopeptides Proteins 0.000 description 4
- 101150040663 PGI1 gene Proteins 0.000 description 4
- 101100395573 Pseudomonas syringae pv. syringae hrmA gene Proteins 0.000 description 4
- 244000061456 Solanum tuberosum Species 0.000 description 4
- 235000002595 Solanum tuberosum Nutrition 0.000 description 4
- 238000002105 Southern blotting Methods 0.000 description 4
- 108700019146 Transgenes Proteins 0.000 description 4
- 238000000429 assembly Methods 0.000 description 4
- 230000000712 assembly Effects 0.000 description 4
- 230000006378 damage Effects 0.000 description 4
- 241001233957 eudicotyledons Species 0.000 description 4
- 238000002474 experimental method Methods 0.000 description 4
- 230000004927 fusion Effects 0.000 description 4
- BRZYSWJRSDMWLG-CAXSIQPQSA-N geneticin Chemical compound O1C[C@@](O)(C)[C@H](NC)[C@@H](O)[C@H]1O[C@@H]1[C@@H](O)[C@H](O[C@@H]2[C@@H]([C@@H](O)[C@H](O)[C@@H](C(C)O)O2)N)[C@@H](N)C[C@H]1N BRZYSWJRSDMWLG-CAXSIQPQSA-N 0.000 description 4
- 230000012010 growth Effects 0.000 description 4
- 230000006698 induction Effects 0.000 description 4
- 239000012528 membrane Substances 0.000 description 4
- 239000000178 monomer Substances 0.000 description 4
- 238000012545 processing Methods 0.000 description 4
- 239000000126 substance Substances 0.000 description 4
- 241000112140 Abaca bunchy top virus Species 0.000 description 3
- 101100519039 Arabidopsis thaliana PBL12 gene Proteins 0.000 description 3
- 244000003416 Asparagus officinalis Species 0.000 description 3
- 235000005340 Asparagus officinalis Nutrition 0.000 description 3
- 241000938605 Crocodylia Species 0.000 description 3
- 108010068370 Glutens Proteins 0.000 description 3
- 102100021181 Golgi phosphoprotein 3 Human genes 0.000 description 3
- 108010043121 Green Fluorescent Proteins Proteins 0.000 description 3
- 102000004144 Green Fluorescent Proteins Human genes 0.000 description 3
- 102000003960 Ligases Human genes 0.000 description 3
- 108090000364 Ligases Proteins 0.000 description 3
- 241000208125 Nicotiana Species 0.000 description 3
- 240000007594 Oryza sativa Species 0.000 description 3
- 235000007164 Oryza sativa Nutrition 0.000 description 3
- 238000012408 PCR amplification Methods 0.000 description 3
- 108090000848 Ubiquitin Proteins 0.000 description 3
- 102000044159 Ubiquitin Human genes 0.000 description 3
- 239000003513 alkali Substances 0.000 description 3
- 125000003275 alpha amino acid group Chemical group 0.000 description 3
- 125000000539 amino acid group Chemical group 0.000 description 3
- 230000008901 benefit Effects 0.000 description 3
- 238000009395 breeding Methods 0.000 description 3
- 230000001488 breeding effect Effects 0.000 description 3
- 235000013339 cereals Nutrition 0.000 description 3
- 210000000349 chromosome Anatomy 0.000 description 3
- 238000004140 cleaning Methods 0.000 description 3
- 125000004122 cyclic group Chemical group 0.000 description 3
- 238000013016 damping Methods 0.000 description 3
- 201000010099 disease Diseases 0.000 description 3
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 3
- 241001493065 dsRNA viruses Species 0.000 description 3
- 229930003944 flavone Natural products 0.000 description 3
- 150000002213 flavones Chemical class 0.000 description 3
- 235000011949 flavones Nutrition 0.000 description 3
- 239000012530 fluid Substances 0.000 description 3
- 235000013305 food Nutrition 0.000 description 3
- 230000001744 histochemical effect Effects 0.000 description 3
- 238000009396 hybridization Methods 0.000 description 3
- 238000001727 in vivo Methods 0.000 description 3
- 230000003834 intracellular effect Effects 0.000 description 3
- JEIPFZHSYJVQDO-UHFFFAOYSA-N iron(III) oxide Inorganic materials O=[Fe]O[Fe]=O JEIPFZHSYJVQDO-UHFFFAOYSA-N 0.000 description 3
- 210000004962 mammalian cell Anatomy 0.000 description 3
- 239000000463 material Substances 0.000 description 3
- 244000005700 microbiome Species 0.000 description 3
- 230000008520 organization Effects 0.000 description 3
- 108091008146 restriction endonucleases Proteins 0.000 description 3
- 235000009566 rice Nutrition 0.000 description 3
- 238000010561 standard procedure Methods 0.000 description 3
- 230000000638 stimulation Effects 0.000 description 3
- 230000002103 transcriptional effect Effects 0.000 description 3
- 238000001890 transfection Methods 0.000 description 3
- 230000009466 transformation Effects 0.000 description 3
- 244000052613 viral pathogen Species 0.000 description 3
- 210000002845 virion Anatomy 0.000 description 3
- 239000000277 virosome Substances 0.000 description 3
- 241001136782 Alca Species 0.000 description 2
- 206010002198 Anaphylactic reaction Diseases 0.000 description 2
- 108020005544 Antisense RNA Proteins 0.000 description 2
- 241000351920 Aspergillus nidulans Species 0.000 description 2
- 108090000994 Catalytic RNA Proteins 0.000 description 2
- 102000053642 Catalytic RNA Human genes 0.000 description 2
- 241001414720 Cicadellidae Species 0.000 description 2
- 244000060011 Cocos nucifera Species 0.000 description 2
- 235000013162 Cocos nucifera Nutrition 0.000 description 2
- JPVYNHNXODAKFH-UHFFFAOYSA-N Cu2+ Chemical compound [Cu+2] JPVYNHNXODAKFH-UHFFFAOYSA-N 0.000 description 2
- 108010005054 Deoxyribonuclease BamHI Proteins 0.000 description 2
- 101150099000 EXPA1 gene Proteins 0.000 description 2
- 102100029095 Exportin-1 Human genes 0.000 description 2
- 108010042546 GCGGCCGC-specific type II deoxyribonucleases Proteins 0.000 description 2
- 108010060309 Glucuronidase Proteins 0.000 description 2
- 102000053187 Glucuronidase Human genes 0.000 description 2
- 241000209510 Liliopsida Species 0.000 description 2
- 235000007688 Lycopersicon esculentum Nutrition 0.000 description 2
- 241000124008 Mammalia Species 0.000 description 2
- 108060004795 Methyltransferase Proteins 0.000 description 2
- 102000008109 Mixed Function Oxygenases Human genes 0.000 description 2
- 108010074633 Mixed Function Oxygenases Proteins 0.000 description 2
- 239000004677 Nylon Substances 0.000 description 2
- 240000000905 Nymphoides indica Species 0.000 description 2
- 235000017590 Nymphoides indica Nutrition 0.000 description 2
- 244000046052 Phaseolus vulgaris Species 0.000 description 2
- 108020005089 Plant RNA Proteins 0.000 description 2
- 241000709769 Potato leafroll virus Species 0.000 description 2
- 241000589624 Pseudomonas amygdali pv. tabaci Species 0.000 description 2
- 108020005067 RNA Splice Sites Proteins 0.000 description 2
- 108020004511 Recombinant DNA Proteins 0.000 description 2
- 108020005091 Replication Origin Proteins 0.000 description 2
- 101100119348 Saccharomyces cerevisiae (strain ATCC 204508 / S288c) EXP1 gene Proteins 0.000 description 2
- 229920002684 Sepharose Polymers 0.000 description 2
- 240000003768 Solanum lycopersicum Species 0.000 description 2
- 101100269618 Streptococcus pneumoniae serotype 4 (strain ATCC BAA-334 / TIGR4) aliA gene Proteins 0.000 description 2
- 241001508381 Subterranean clover stunt virus Species 0.000 description 2
- 241000723573 Tobacco rattle virus Species 0.000 description 2
- 241000723790 Tobacco vein mottling virus Species 0.000 description 2
- 108700009124 Transcription Initiation Site Proteins 0.000 description 2
- 241000219870 Trifolium subterraneum Species 0.000 description 2
- 108060008682 Tumor Necrosis Factor Proteins 0.000 description 2
- 108010084455 Zeocin Proteins 0.000 description 2
- 230000001594 aberrant effect Effects 0.000 description 2
- 239000002253 acid Substances 0.000 description 2
- 230000003213 activating effect Effects 0.000 description 2
- 235000001014 amino acid Nutrition 0.000 description 2
- 150000001413 amino acids Chemical class 0.000 description 2
- 230000036783 anaphylactic response Effects 0.000 description 2
- 208000003455 anaphylaxis Diseases 0.000 description 2
- 210000004507 artificial chromosome Anatomy 0.000 description 2
- 230000001580 bacterial effect Effects 0.000 description 2
- 235000021015 bananas Nutrition 0.000 description 2
- 230000002950 deficient Effects 0.000 description 2
- 238000011161 development Methods 0.000 description 2
- 230000018109 developmental process Effects 0.000 description 2
- 230000008034 disappearance Effects 0.000 description 2
- 238000007599 discharging Methods 0.000 description 2
- 239000000428 dust Substances 0.000 description 2
- 238000004043 dyeing Methods 0.000 description 2
- 238000001962 electrophoresis Methods 0.000 description 2
- 108010000306 endodeoxyribonuclease PaeI Proteins 0.000 description 2
- 238000005516 engineering process Methods 0.000 description 2
- 108700002148 exportin 1 Proteins 0.000 description 2
- 239000000284 extract Substances 0.000 description 2
- 238000010413 gardening Methods 0.000 description 2
- 238000010353 genetic engineering Methods 0.000 description 2
- 235000021312 gluten Nutrition 0.000 description 2
- PCHJSUWPFVWCPO-UHFFFAOYSA-N gold Chemical compound [Au] PCHJSUWPFVWCPO-UHFFFAOYSA-N 0.000 description 2
- 239000010931 gold Substances 0.000 description 2
- 229910052737 gold Inorganic materials 0.000 description 2
- 101150018478 hrmA gene Proteins 0.000 description 2
- 230000036039 immunity Effects 0.000 description 2
- 230000006872 improvement Effects 0.000 description 2
- 238000011081 inoculation Methods 0.000 description 2
- 230000010354 integration Effects 0.000 description 2
- 230000000968 intestinal effect Effects 0.000 description 2
- 101150066555 lacZ gene Proteins 0.000 description 2
- 239000002502 liposome Substances 0.000 description 2
- 238000004519 manufacturing process Methods 0.000 description 2
- 230000035800 maturation Effects 0.000 description 2
- VNWKTOKETHGBQD-UHFFFAOYSA-N methane Natural products C VNWKTOKETHGBQD-UHFFFAOYSA-N 0.000 description 2
- -1 methane amide Chemical class 0.000 description 2
- 239000000203 mixture Substances 0.000 description 2
- 230000004048 modification Effects 0.000 description 2
- 238000012986 modification Methods 0.000 description 2
- 229920001778 nylon Polymers 0.000 description 2
- 230000002018 overexpression Effects 0.000 description 2
- CWCMIVBLVUHDHK-ZSNHEYEWSA-N phleomycin D1 Chemical compound N([C@H](C(=O)N[C@H](C)[C@@H](O)[C@H](C)C(=O)N[C@@H]([C@H](O)C)C(=O)NCCC=1SC[C@@H](N=1)C=1SC=C(N=1)C(=O)NCCCCNC(N)=N)[C@@H](O[C@H]1[C@H]([C@@H](O)[C@H](O)[C@H](CO)O1)O[C@@H]1[C@H]([C@@H](OC(N)=O)[C@H](O)[C@@H](CO)O1)O)C=1N=CNC=1)C(=O)C1=NC([C@H](CC(N)=O)NC[C@H](N)C(N)=O)=NC(N)=C1C CWCMIVBLVUHDHK-ZSNHEYEWSA-N 0.000 description 2
- 230000037039 plant physiology Effects 0.000 description 2
- 239000002243 precursor Substances 0.000 description 2
- 230000008569 process Effects 0.000 description 2
- 230000001737 promoting effect Effects 0.000 description 2
- 230000000644 propagated effect Effects 0.000 description 2
- 238000000746 purification Methods 0.000 description 2
- 230000009467 reduction Effects 0.000 description 2
- 230000008929 regeneration Effects 0.000 description 2
- 238000011069 regeneration method Methods 0.000 description 2
- 230000003362 replicative effect Effects 0.000 description 2
- 230000002441 reversible effect Effects 0.000 description 2
- 108091092562 ribozyme Proteins 0.000 description 2
- 229910001753 sapphirine Inorganic materials 0.000 description 2
- 241000894007 species Species 0.000 description 2
- 238000012546 transfer Methods 0.000 description 2
- 230000001052 transient effect Effects 0.000 description 2
- 230000010474 transient expression Effects 0.000 description 2
- 230000007704 transition Effects 0.000 description 2
- 210000003462 vein Anatomy 0.000 description 2
- 239000005723 virus inoculator Substances 0.000 description 2
- FEFNLMIFMCISIM-UHFFFAOYSA-N 1-methyl-4-nitro-5-(4-nitrophenyl)sulfanylimidazole Chemical compound CN1C=NC([N+]([O-])=O)=C1SC1=CC=C([N+]([O-])=O)C=C1 FEFNLMIFMCISIM-UHFFFAOYSA-N 0.000 description 1
- 241000251468 Actinopterygii Species 0.000 description 1
- 241000702449 African cassava mosaic virus Species 0.000 description 1
- 102000009027 Albumins Human genes 0.000 description 1
- 108010088751 Albumins Proteins 0.000 description 1
- QGZKDVFQNNGYKY-UHFFFAOYSA-O Ammonium Chemical compound [NH4+] QGZKDVFQNNGYKY-UHFFFAOYSA-O 0.000 description 1
- 241001600407 Aphis <genus> Species 0.000 description 1
- 241000219195 Arabidopsis thaliana Species 0.000 description 1
- 241000239223 Arachnida Species 0.000 description 1
- 241000271566 Aves Species 0.000 description 1
- 108010006654 Bleomycin Proteins 0.000 description 1
- 102000004506 Blood Proteins Human genes 0.000 description 1
- 108010017384 Blood Proteins Proteins 0.000 description 1
- 235000011299 Brassica oleracea var botrytis Nutrition 0.000 description 1
- 240000003259 Brassica oleracea var. botrytis Species 0.000 description 1
- 108010031540 CCGCGG-specific type II deoxyribonucleases Proteins 0.000 description 1
- 108010072454 CTGCAG-specific type II deoxyribonucleases Proteins 0.000 description 1
- 101100128404 Caenorhabditis elegans lir-3 gene Proteins 0.000 description 1
- 241000282461 Canis lupus Species 0.000 description 1
- 102000014914 Carrier Proteins Human genes 0.000 description 1
- 108010078791 Carrier Proteins Proteins 0.000 description 1
- 241000207199 Citrus Species 0.000 description 1
- 241000724711 Coconut foliar decay virus Species 0.000 description 1
- 108020004705 Codon Proteins 0.000 description 1
- 108020004635 Complementary DNA Proteins 0.000 description 1
- 206010010144 Completed suicide Diseases 0.000 description 1
- 229920000742 Cotton Polymers 0.000 description 1
- RDFLLVCQYHQOBU-GPGGJFNDSA-O Cyanin Natural products O([C@H]1[C@H](O)[C@H](O)[C@H](O)[C@H](CO)O1)c1c(-c2cc(O)c(O)cc2)[o+]c2c(c(O[C@H]3[C@H](O)[C@@H](O)[C@H](O)[C@H](CO)O3)cc(O)c2)c1 RDFLLVCQYHQOBU-GPGGJFNDSA-O 0.000 description 1
- 102000004127 Cytokines Human genes 0.000 description 1
- 108090000695 Cytokines Proteins 0.000 description 1
- IMXSCCDUAFEIOE-RITPCOANSA-N D-octopine Chemical class [O-]C(=O)[C@@H](C)[NH2+][C@H](C([O-])=O)CCCNC(N)=[NH2+] IMXSCCDUAFEIOE-RITPCOANSA-N 0.000 description 1
- 102000004594 DNA Polymerase I Human genes 0.000 description 1
- 108010017826 DNA Polymerase I Proteins 0.000 description 1
- 241000450599 DNA viruses Species 0.000 description 1
- 102000016928 DNA-directed DNA polymerase Human genes 0.000 description 1
- 108010014303 DNA-directed DNA polymerase Proteins 0.000 description 1
- 108010054576 Deoxyribonuclease EcoRI Proteins 0.000 description 1
- 108091027757 Deoxyribozyme Proteins 0.000 description 1
- 238000002965 ELISA Methods 0.000 description 1
- 241000701832 Enterobacteria phage T3 Species 0.000 description 1
- YQYJSBFKSSDGFO-UHFFFAOYSA-N Epihygromycin Natural products OC1C(O)C(C(=O)C)OC1OC(C(=C1)O)=CC=C1C=C(C)C(=O)NC1C(O)C(O)C2OCOC2C1O YQYJSBFKSSDGFO-UHFFFAOYSA-N 0.000 description 1
- 241000588724 Escherichia coli Species 0.000 description 1
- 241000710831 Flavivirus Species 0.000 description 1
- 101150066002 GFP gene Proteins 0.000 description 1
- 108010026624 GTCGAC-specific type II deoxyribonucleases Proteins 0.000 description 1
- 108700039691 Genetic Promoter Regions Proteins 0.000 description 1
- 206010020649 Hyperkeratosis Diseases 0.000 description 1
- 108090000723 Insulin-Like Growth Factor I Proteins 0.000 description 1
- 102000014150 Interferons Human genes 0.000 description 1
- 108010050904 Interferons Proteins 0.000 description 1
- 206010023198 Joint ankylosis Diseases 0.000 description 1
- 108010025815 Kanamycin Kinase Proteins 0.000 description 1
- 108060001084 Luciferase Proteins 0.000 description 1
- 108700005084 Multigene Family Proteins 0.000 description 1
- 241001467086 Mungbean yellow mosaic virus Species 0.000 description 1
- PEASFKSPITUZGT-UHFFFAOYSA-N N-phenyl-N-[1-(2-phenylethyl)piperidin-4-yl]cyclopentanecarboxamide Chemical compound O=C(C1CCCC1)N(C1CCN(CCC2=CC=CC=C2)CC1)C1=CC=CC=C1 PEASFKSPITUZGT-UHFFFAOYSA-N 0.000 description 1
- 241000244206 Nematoda Species 0.000 description 1
- 241000325773 Orosius argentatus Species 0.000 description 1
- 241000283973 Oryctolagus cuniculus Species 0.000 description 1
- 241000381142 Pachydermia Species 0.000 description 1
- 241001220303 Paratrichodorus pachydermus Species 0.000 description 1
- 206010034133 Pathogen resistance Diseases 0.000 description 1
- 235000010627 Phaseolus vulgaris Nutrition 0.000 description 1
- IAJOBQBIJHVGMQ-UHFFFAOYSA-N Phosphinothricin Natural products CP(O)(=O)CCC(N)C(O)=O IAJOBQBIJHVGMQ-UHFFFAOYSA-N 0.000 description 1
- 108091000080 Phosphotransferase Proteins 0.000 description 1
- 241000233629 Phytophthora parasitica Species 0.000 description 1
- 108020005120 Plant DNA Proteins 0.000 description 1
- 108700001094 Plant Genes Proteins 0.000 description 1
- 241000288906 Primates Species 0.000 description 1
- 241000589615 Pseudomonas syringae Species 0.000 description 1
- 241000589623 Pseudomonas syringae pv. syringae Species 0.000 description 1
- 108091034057 RNA (poly(A)) Proteins 0.000 description 1
- 102000018120 Recombinases Human genes 0.000 description 1
- 108010091086 Recombinases Proteins 0.000 description 1
- 101710090029 Replication-associated protein A Proteins 0.000 description 1
- 201000000582 Retinoblastoma Diseases 0.000 description 1
- 108050002653 Retinoblastoma protein Proteins 0.000 description 1
- 108010003581 Ribulose-bisphosphate carboxylase Proteins 0.000 description 1
- 230000018199 S phase Effects 0.000 description 1
- 240000004808 Saccharomyces cerevisiae Species 0.000 description 1
- 206010039509 Scab Diseases 0.000 description 1
- 102000013275 Somatomedins Human genes 0.000 description 1
- 244000223014 Syzygium aromaticum Species 0.000 description 1
- 235000016639 Syzygium aromaticum Nutrition 0.000 description 1
- 108700026226 TATA Box Proteins 0.000 description 1
- 239000004098 Tetracycline Substances 0.000 description 1
- AUYYCJSJGJYCDS-LBPRGKRZSA-N Thyrolar Chemical compound IC1=CC(C[C@H](N)C(O)=O)=CC(I)=C1OC1=CC=C(O)C(I)=C1 AUYYCJSJGJYCDS-LBPRGKRZSA-N 0.000 description 1
- 101710183280 Topoisomerase Proteins 0.000 description 1
- 235000021307 Triticum Nutrition 0.000 description 1
- 244000098338 Triticum aestivum Species 0.000 description 1
- 240000006677 Vicia faba Species 0.000 description 1
- 235000010749 Vicia faba Nutrition 0.000 description 1
- 235000002098 Vicia faba var. major Nutrition 0.000 description 1
- 240000004922 Vigna radiata Species 0.000 description 1
- 235000010721 Vigna radiata var radiata Nutrition 0.000 description 1
- 235000011469 Vigna radiata var sublobata Nutrition 0.000 description 1
- 108020005202 Viral DNA Proteins 0.000 description 1
- 108050006628 Viral movement proteins Proteins 0.000 description 1
- 241000702302 Wheat dwarf virus Species 0.000 description 1
- 235000016383 Zea mays subsp huehuetenangensis Nutrition 0.000 description 1
- 108010055615 Zein Proteins 0.000 description 1
- 150000007513 acids Chemical class 0.000 description 1
- 230000009471 action Effects 0.000 description 1
- 229960000723 ampicillin Drugs 0.000 description 1
- AVKUERGKIZMTKX-NJBDSQKTSA-N ampicillin Chemical compound C1([C@@H](N)C(=O)N[C@H]2[C@H]3SC([C@@H](N3C2=O)C(O)=O)(C)C)=CC=CC=C1 AVKUERGKIZMTKX-NJBDSQKTSA-N 0.000 description 1
- 210000004102 animal cell Anatomy 0.000 description 1
- 239000003242 anti bacterial agent Substances 0.000 description 1
- 229940088710 antibiotic agent Drugs 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 229960001561 bleomycin Drugs 0.000 description 1
- OYVAGSVQBOHSSS-UAPAGMARSA-O bleomycin A2 Chemical compound N([C@H](C(=O)N[C@H](C)[C@@H](O)[C@H](C)C(=O)N[C@@H]([C@H](O)C)C(=O)NCCC=1SC=C(N=1)C=1SC=C(N=1)C(=O)NCCC[S+](C)C)[C@@H](O[C@H]1[C@H]([C@@H](O)[C@H](O)[C@H](CO)O1)O[C@@H]1[C@H]([C@@H](OC(N)=O)[C@H](O)[C@@H](CO)O1)O)C=1N=CNC=1)C(=O)C1=NC([C@H](CC(N)=O)NC[C@H](N)C(N)=O)=NC(N)=C1C OYVAGSVQBOHSSS-UAPAGMARSA-O 0.000 description 1
- 238000010804 cDNA synthesis Methods 0.000 description 1
- 229940027138 cambia Drugs 0.000 description 1
- 230000010307 cell transformation Effects 0.000 description 1
- 238000012512 characterization method Methods 0.000 description 1
- 238000013375 chromatographic separation Methods 0.000 description 1
- 235000020971 citrus fruits Nutrition 0.000 description 1
- 239000004927 clay Substances 0.000 description 1
- 239000011248 coating agent Substances 0.000 description 1
- 238000000576 coating method Methods 0.000 description 1
- 230000000052 comparative effect Effects 0.000 description 1
- 150000001875 compounds Chemical class 0.000 description 1
- 238000004590 computer program Methods 0.000 description 1
- 238000012790 confirmation Methods 0.000 description 1
- 239000000470 constituent Substances 0.000 description 1
- 238000010276 construction Methods 0.000 description 1
- 238000005336 cracking Methods 0.000 description 1
- 238000012258 culturing Methods 0.000 description 1
- RDFLLVCQYHQOBU-ZOTFFYTFSA-O cyanin Chemical compound O[C@@H]1[C@@H](O)[C@H](O)[C@@H](CO)O[C@H]1OC(C(=[O+]C1=CC(O)=C2)C=3C=C(O)C(O)=CC=3)=CC1=C2O[C@H]1[C@H](O)[C@@H](O)[C@H](O)[C@@H](CO)O1 RDFLLVCQYHQOBU-ZOTFFYTFSA-O 0.000 description 1
- 230000001086 cytosolic effect Effects 0.000 description 1
- 230000034994 death Effects 0.000 description 1
- 238000012217 deletion Methods 0.000 description 1
- 230000037430 deletion Effects 0.000 description 1
- 238000013461 design Methods 0.000 description 1
- 230000009025 developmental regulation Effects 0.000 description 1
- KXZOIWWTXOCYKR-UHFFFAOYSA-M diclofenac potassium Chemical compound [K+].[O-]C(=O)CC1=CC=CC=C1NC1=C(Cl)C=CC=C1Cl KXZOIWWTXOCYKR-UHFFFAOYSA-M 0.000 description 1
- 238000007598 dipping method Methods 0.000 description 1
- UKWLRLAKGMZXJC-QIECWBMSSA-L disodium;[4-chloro-3-[(3r,5s)-1-chloro-3'-methoxyspiro[adamantane-4,4'-dioxetane]-3'-yl]phenyl] phosphate Chemical compound [Na+].[Na+].O1OC2([C@@H]3CC4C[C@H]2CC(Cl)(C4)C3)C1(OC)C1=CC(OP([O-])([O-])=O)=CC=C1Cl UKWLRLAKGMZXJC-QIECWBMSSA-L 0.000 description 1
- 239000003814 drug Substances 0.000 description 1
- 235000013399 edible fruits Nutrition 0.000 description 1
- 108010003117 endodeoxyribonuclease BstYI Proteins 0.000 description 1
- 108010021310 endodeoxyribonuclease NcoI Proteins 0.000 description 1
- 108010002480 endodeoxyribonuclease SacI Proteins 0.000 description 1
- 210000002615 epidermis Anatomy 0.000 description 1
- 238000011156 evaluation Methods 0.000 description 1
- 210000003722 extracellular fluid Anatomy 0.000 description 1
- 230000002349 favourable effect Effects 0.000 description 1
- 239000000835 fiber Substances 0.000 description 1
- 238000005558 fluorometry Methods 0.000 description 1
- 238000009472 formulation Methods 0.000 description 1
- 230000030279 gene silencing Effects 0.000 description 1
- 238000012226 gene silencing method Methods 0.000 description 1
- 230000008303 genetic mechanism Effects 0.000 description 1
- IAJOBQBIJHVGMQ-BYPYZUCNSA-N glufosinate-P Chemical compound CP(O)(=O)CC[C@H](N)C(O)=O IAJOBQBIJHVGMQ-BYPYZUCNSA-N 0.000 description 1
- 229920006158 high molecular weight polymer Polymers 0.000 description 1
- 239000005556 hormone Substances 0.000 description 1
- 229940088597 hormone Drugs 0.000 description 1
- 238000003898 horticulture Methods 0.000 description 1
- 101150026546 hsa gene Proteins 0.000 description 1
- 230000002458 infectious effect Effects 0.000 description 1
- 230000001524 infective effect Effects 0.000 description 1
- 230000000977 initiatory effect Effects 0.000 description 1
- 208000014674 injury Diseases 0.000 description 1
- 238000003780 insertion Methods 0.000 description 1
- 230000037431 insertion Effects 0.000 description 1
- 229940079322 interferon Drugs 0.000 description 1
- XUWPJKDMEZSVTP-LTYMHZPRSA-N kalafungina Chemical compound O=C1C2=C(O)C=CC=C2C(=O)C2=C1[C@@H](C)O[C@H]1[C@@H]2OC(=O)C1 XUWPJKDMEZSVTP-LTYMHZPRSA-N 0.000 description 1
- 229960000318 kanamycin Drugs 0.000 description 1
- 229930027917 kanamycin Natural products 0.000 description 1
- SBUJHOSQTJFQJX-NOAMYHISSA-N kanamycin Chemical compound O[C@@H]1[C@@H](O)[C@H](O)[C@@H](CN)O[C@@H]1O[C@H]1[C@H](O)[C@@H](O[C@@H]2[C@@H]([C@@H](N)[C@H](O)[C@@H](CO)O2)O)[C@H](N)C[C@@H]1N SBUJHOSQTJFQJX-NOAMYHISSA-N 0.000 description 1
- 229930182823 kanamycin A Natural products 0.000 description 1
- 150000002632 lipids Chemical class 0.000 description 1
- 235000009973 maize Nutrition 0.000 description 1
- 238000005259 measurement Methods 0.000 description 1
- 239000002207 metabolite Substances 0.000 description 1
- 229910021645 metal ion Inorganic materials 0.000 description 1
- 238000000520 microinjection Methods 0.000 description 1
- 238000002156 mixing Methods 0.000 description 1
- 229940031815 mycocide Drugs 0.000 description 1
- 238000007857 nested PCR Methods 0.000 description 1
- 102000020233 phosphotransferase Human genes 0.000 description 1
- 230000008635 plant growth Effects 0.000 description 1
- 244000000003 plant pathogen Species 0.000 description 1
- 229920000642 polymer Polymers 0.000 description 1
- 101150038105 pr gene Proteins 0.000 description 1
- 230000001376 precipitating effect Effects 0.000 description 1
- 238000001556 precipitation Methods 0.000 description 1
- 238000002360 preparation method Methods 0.000 description 1
- 230000006798 recombination Effects 0.000 description 1
- 238000005215 recombination Methods 0.000 description 1
- 230000001172 regenerating effect Effects 0.000 description 1
- 230000008521 reorganization Effects 0.000 description 1
- 238000012827 research and development Methods 0.000 description 1
- 238000007363 ring formation reaction Methods 0.000 description 1
- 238000012216 screening Methods 0.000 description 1
- 238000012882 sequential analysis Methods 0.000 description 1
- 238000004904 shortening Methods 0.000 description 1
- 230000003584 silencer Effects 0.000 description 1
- 238000004088 simulation Methods 0.000 description 1
- 150000003431 steroids Chemical class 0.000 description 1
- 230000004936 stimulating effect Effects 0.000 description 1
- 239000000758 substrate Substances 0.000 description 1
- 230000021918 systemic acquired resistance Effects 0.000 description 1
- 229960002180 tetracycline Drugs 0.000 description 1
- 229930101283 tetracycline Natural products 0.000 description 1
- 235000019364 tetracycline Nutrition 0.000 description 1
- 150000003522 tetracyclines Chemical class 0.000 description 1
- 230000001225 therapeutic effect Effects 0.000 description 1
- 238000003151 transfection method Methods 0.000 description 1
- 238000011426 transformation method Methods 0.000 description 1
- 229940035722 triiodothyronine Drugs 0.000 description 1
- 210000005253 yeast cell Anatomy 0.000 description 1
Images
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
- C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
- C12N15/8261—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield
- C12N15/8271—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance
- C12N15/8279—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance for biotic stress resistance, pathogen resistance, disease resistance
- C12N15/8282—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance for biotic stress resistance, pathogen resistance, disease resistance for fungal resistance
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
- C12N15/8201—Methods for introducing genetic material into plant cells, e.g. DNA, RNA, stable or transient incorporation, tissue culture methods adapted for transformation
- C12N15/8202—Methods for introducing genetic material into plant cells, e.g. DNA, RNA, stable or transient incorporation, tissue culture methods adapted for transformation by biological means, e.g. cell mediated or natural vector
- C12N15/8203—Virus mediated transformation
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
- C12N15/8216—Methods for controlling, regulating or enhancing expression of transgenes in plant cells
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
- C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
- C12N15/8242—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits
- C12N15/8257—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits for the production of primary gene products, e.g. pharmaceutical products, interferon
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
- C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
- C12N15/8261—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield
- C12N15/8262—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield involving plant development
- C12N15/8263—Ablation; Apoptosis
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
- C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
- C12N15/8261—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield
- C12N15/8271—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance
- C12N15/8279—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance for biotic stress resistance, pathogen resistance, disease resistance
- C12N15/8283—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance for biotic stress resistance, pathogen resistance, disease resistance for virus resistance
Landscapes
- Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Biomedical Technology (AREA)
- Chemical & Material Sciences (AREA)
- Biotechnology (AREA)
- Zoology (AREA)
- Organic Chemistry (AREA)
- General Engineering & Computer Science (AREA)
- Wood Science & Technology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Molecular Biology (AREA)
- Microbiology (AREA)
- Plant Pathology (AREA)
- Physics & Mathematics (AREA)
- Biophysics (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- Cell Biology (AREA)
- Virology (AREA)
- Medicinal Chemistry (AREA)
- Pharmacology & Pharmacy (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
- Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
- Arrangement And Driving Of Transmission Devices (AREA)
- Peptides Or Proteins (AREA)
Abstract
Description
序列标识符 | 描述 |
SEQ ID NO:1到SEQ ID NO:18 | 合成的寡聚核苷酸 |
SEQ ID NO:19到SEQ ID NO:44 | 引物 |
SEQ ID NO:45到SEQ ID NO:52 | 合成的寡聚核苷酸 |
SEQ ID NO:66 | 芽孢杆菌RNA酶pTBN |
SEQ ID NO:67 | 芽孢杆菌RNA酶pRTBN6 |
SEQ ID NO:68 | 芽孢杆菌RNA酶pRTBN1 |
SEQ ID NO:69 | GFPpGI |
SEQ ID NO:70 | GFPpGI6 |
SEQ ID NO:71 | GFPpGI1 |
SEQ ID NO:72 | 引物 |
SEQ ID NO:73 | 引物 |
引物 | 名称 | 序列(5′-3′) |
+1 | 3TS1-H | AAGCTTCATGGAGTCAAAGA (SEQ ID NO:1) |
+2 | 35S1-B | TCATTTGGAGAGGATCCATGGCACAGGTT (SEQ ID NO:2) |
-2 | B2-35S | AACCTGTGCCATGGATCCTCTCCAAATGA (SEQ ID NO:3) |
+3 | B1-N | AAAATCAGATAAGAGCTCGATCGTTCAAA (SEQ ID NO:4) |
-3 | N2-B | TTTGAACGATCGAGCTCTTATCTGATTTT (SEQ ID NO:5) |
-4 | NOS4-H | AAGCTTTTCGCCATTCAGGCTGC (SEQ ID NO:6) |
+5 | B3-6 | TATCATTAATTAGTAAGTTGTGCTGTAA (SEQ ID NO:7) |
-5 | 6-B4 | TTACAGCACAACTTACTAATTAATGATA (SEQ ID NO:8) |
+6 | 6-B3 | GGAAGGCAGAAGCGAGTAATATAATATT (SEQ ID NO:9) |
-6 | B4-6 | AATATTATATTACTCGCTTCTGCCTTCC (SEQ ID NO:10) |
+7 | B5-I | ATCATTAATTAGTCACACTATGACAAAAG (SEQ ID NO:11) |
-7 | I-B6 | TTGTCATAGTGTGACTAATTAATGATAAT (SEQ ID NO:12) |
+8 | I-B5 | GACATTTGCATCAGTAATATAATATTTCA (SEQ ID NO:13) |
-8 | B6-I | AATATTATATTACTGATGCAAATGTCCCG (SEQ ID NO:14) |
+9 | B7-6T | TCAGATAAGAGCTCAGTAACAGCAACAAC (SEQ ID NO:15) |
-9 | 6T2-B | GCTGTTACTGAGCTCTTATCTGATCTTTG (SEQ ID NO:16) |
-10 | 6T4-H | AAGCTTATTTCCCAAATATACGT (SEQ ID NO:17) |
+11 | UNTBarn | GGATCCGCACAGGTTATCAAC (SEQ ID NO:18) |
x-轴标准(图15) | RTBN6 | RUBN6 | RTBN1 | RUBN1 |
BBTV1 | √ | √ | √ | √ |
BBTV5 | √ | √ | √ | √ |
GUS | √ | √ | √ | √ |
RTBN6 | √ | - | - | - |
RUBN6 | - | √ | - | - |
RTBN1 | - | - | √ | - |
RUBN1 | - | - | - | √ |
x-轴标准(图16) | RTBN6+ | RTBN6- | RUBN6+ | RTBN1+ | RTBN1- | RUBN1+ |
BBTV1 | √ | - | √ | √ | - | √ |
BBTV5 | √ | √ | √ | √ | √ | √ |
GUS | √ | √ | √ | √ | √ | √ |
RTBN6 | √ | √ | - | - | - | - |
RUBN6 | - | - | √ | - | - | - |
RTBN1 | - | - | - | √ | √ | - |
RUBN1 | - | - | - | - | - | √ |
Stuffer | - | √ | - | - | √ | - |
质粒组合 | p35S-BTRpWORM | p35S-BUTRpWORM | pBTR.test1 pWORM | pBUTR.test1 pWORM | pBTR.test1pWORMp35S-Rep | pBUTR.test1 pWORMp35S-Rep |
轰击后3日的绿色荧光转化灶 | 否 | 是 | 是 | 是 | 否 | 是 |
引物 | 名称 | 序列(5′-3′) |
+1 | 35SH | AAGCTTCATGGAGTCAAAGA(SEQ ID NO:45) |
+2 | 5′mGFPBam | GGATCCATGAGTAAAGGAGAAGAACTT (SEQ ID NO:46) |
+3 | GFPB1 | AAGTCAAGTTTGAGGTAAGTTTCTGCTTC (SEQ ID NO:47) |
-3 | B2GFP | GAAGCAGAAACTTACCTCAAACTTGACTT (SEQ ID NO:448) |
+4 | B1GFP | TTGTTGATGTGCAGGGAGACACCCTCGTC (SEQ ID NO:49) |
-4 | GFPB2 | GACGAGGGTGTCTCCCTGCACATCAACAA (SEQ ID NO:50) |
-5 | 3′mGFPSac | GAGCTCTTATTTGTATAGTTCATCCAT (SEQ ID NO:51) |
-6 | NOS4-H | AAGCTTTTCGCCATTCAGGCTGC (SEQ ID NO:52) |
+7 | B3-6 | TATCATTAATTAGTAAGTTGTGCTGTAA (SEQ ID NO:53) |
-8 | B4-6 | AATATTATATTACTCGCTTCTGCCTTCC (SEQ ID NO:54) |
+9 | B5-I | ATCATTAATTAGTCACACTATGACAAAAG (SEQ ID NO:55) |
-10 | B6-I | AATATTATATTACTGATGCAAATGTCCCCG (SEQ ID NO:56) |
Claims (40)
Applications Claiming Priority (5)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
AUPQ6516 | 2000-03-28 | ||
AUPQ6516A AUPQ651600A0 (en) | 2000-03-28 | 2000-03-28 | Suicide gene vector |
AUPR1081A AUPR108100A0 (en) | 2000-10-27 | 2000-10-27 | A construct |
AUPR1081 | 2000-10-27 | ||
PCT/AU2001/000349 WO2001072996A1 (en) | 2000-03-28 | 2001-03-28 | A construct capable of release in closed circular form from a larger nucleotide sequence permitting site specific expression and/or developmentally regulated expression of selected genetic sequences |
Publications (2)
Publication Number | Publication Date |
---|---|
CN1426465A true CN1426465A (zh) | 2003-06-25 |
CN1426465B CN1426465B (zh) | 2016-01-20 |
Family
ID=25646287
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
CN01808621.7A Expired - Lifetime CN1426465B (zh) | 2000-03-28 | 2001-03-28 | 能从较大的核苷酸序列中以闭合环状形式释放,并且允许位点特异性地表达和/或发育调控性地表达所选遗传序列的构建体 |
Country Status (16)
Country | Link |
---|---|
US (2) | US7863430B2 (zh) |
EP (1) | EP1282697B1 (zh) |
JP (1) | JP4797141B2 (zh) |
KR (1) | KR20030047880A (zh) |
CN (1) | CN1426465B (zh) |
AP (2) | AP1645A (zh) |
AT (1) | ATE376589T1 (zh) |
AU (1) | AU4393901A (zh) |
BR (1) | BRPI0109522B8 (zh) |
CA (1) | CA2404471C (zh) |
DE (1) | DE60131075T2 (zh) |
ES (1) | ES2296736T3 (zh) |
MX (1) | MXPA02009339A (zh) |
NZ (1) | NZ521488A (zh) |
PT (1) | PT1282697E (zh) |
WO (1) | WO2001072996A1 (zh) |
Families Citing this family (9)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US8497127B2 (en) | 2007-12-03 | 2013-07-30 | Syngenta Participations Ag | Engineering enzymatically susceptible proteins |
US8901372B2 (en) * | 2009-02-25 | 2014-12-02 | University Of Hawaii | Plant resistance to banana bunchy top virus |
EP2996697B1 (en) | 2013-05-15 | 2019-06-26 | Robert Kruse | Intracellular translation of circular rna |
GB201402249D0 (en) | 2014-02-10 | 2014-03-26 | Vela Operations Pte Ltd | NGS systems control and methods involving the same |
WO2017136895A1 (en) * | 2016-02-10 | 2017-08-17 | Queensland University Of Technology | Constructs and methods for conferring virus resistance |
WO2019236673A1 (en) | 2018-06-06 | 2019-12-12 | Massachusetts Institute Of Technology | Circular rna for translation in eukaryotic cells |
CN115806976A (zh) * | 2019-03-20 | 2023-03-17 | 中国科学院上海营养与健康研究所 | 利用环形rna进行蛋白翻译及其应用 |
IL310266A (en) | 2019-05-22 | 2024-03-01 | Massachusetts Inst Technology | Circular Rana preparations and methods |
CN115052635A (zh) | 2019-12-04 | 2022-09-13 | 奥纳治疗公司 | 环状rna组合物和方法 |
Family Cites Families (3)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
AUPN328595A0 (en) * | 1995-05-31 | 1995-06-22 | Queensland University Of Technology | Intergenic regions of banana bunchy top virus |
US6077992A (en) * | 1997-10-24 | 2000-06-20 | E. I. Du Pont De Nemours And Company | Binary viral expression system in plants |
NZ510594A (en) | 1998-09-23 | 2002-09-27 | Du Pont | Binary viral expression system in plants |
-
2001
- 2001-03-28 DE DE60131075T patent/DE60131075T2/de not_active Expired - Lifetime
- 2001-03-28 MX MXPA02009339A patent/MXPA02009339A/es active IP Right Grant
- 2001-03-28 NZ NZ521488A patent/NZ521488A/en not_active IP Right Cessation
- 2001-03-28 JP JP2001570714A patent/JP4797141B2/ja not_active Expired - Fee Related
- 2001-03-28 ES ES01916742T patent/ES2296736T3/es not_active Expired - Lifetime
- 2001-03-28 PT PT01916742T patent/PT1282697E/pt unknown
- 2001-03-28 AP APAP/P/2002/002636D patent/AP1645A/en active
- 2001-03-28 KR KR1020027012740A patent/KR20030047880A/ko not_active Application Discontinuation
- 2001-03-28 AT AT01916742T patent/ATE376589T1/de active
- 2001-03-28 EP EP01916742A patent/EP1282697B1/en not_active Expired - Lifetime
- 2001-03-28 AP APAP/P/2001/002105A patent/AP1440A/en active
- 2001-03-28 WO PCT/AU2001/000349 patent/WO2001072996A1/en active IP Right Grant
- 2001-03-28 CA CA2404471A patent/CA2404471C/en not_active Expired - Fee Related
- 2001-03-28 US US10/168,653 patent/US7863430B2/en not_active Expired - Fee Related
- 2001-03-28 AU AU4393901A patent/AU4393901A/xx active Pending
- 2001-03-28 CN CN01808621.7A patent/CN1426465B/zh not_active Expired - Lifetime
- 2001-03-28 BR BRPI0109522A patent/BRPI0109522B8/pt not_active IP Right Cessation
-
2010
- 2010-11-17 US US12/948,005 patent/US8829170B2/en not_active Expired - Fee Related
Also Published As
Publication number | Publication date |
---|---|
BRPI0109522B1 (pt) | 2015-12-08 |
AP1440A (en) | 2005-06-28 |
CA2404471C (en) | 2010-09-28 |
JP4797141B2 (ja) | 2011-10-19 |
MXPA02009339A (es) | 2004-05-14 |
EP1282697A4 (en) | 2004-06-23 |
KR20030047880A (ko) | 2003-06-18 |
US20040121430A1 (en) | 2004-06-24 |
NZ521488A (en) | 2004-07-30 |
WO2001072996A1 (en) | 2001-10-04 |
AP1645A (en) | 2006-07-28 |
US7863430B2 (en) | 2011-01-04 |
AP2002002636A0 (en) | 2002-09-30 |
US8829170B2 (en) | 2014-09-09 |
ES2296736T3 (es) | 2008-05-01 |
US20110119782A1 (en) | 2011-05-19 |
CN1426465B (zh) | 2016-01-20 |
DE60131075D1 (de) | 2007-12-06 |
PT1282697E (pt) | 2008-02-01 |
DE60131075T2 (de) | 2008-07-31 |
AU4393901A (en) | 2001-10-08 |
CA2404471A1 (en) | 2001-10-04 |
EP1282697B1 (en) | 2007-10-24 |
ATE376589T1 (de) | 2007-11-15 |
BR0109522A (pt) | 2003-06-10 |
EP1282697A1 (en) | 2003-02-12 |
JP2003528606A (ja) | 2003-09-30 |
BRPI0109522B8 (pt) | 2017-04-11 |
Similar Documents
Publication | Publication Date | Title |
---|---|---|
CN1164869A (zh) | 来源于环病毒的植物转录调节物 | |
CN1202246C (zh) | 获得修饰表型的方法和措施 | |
CN1214110C (zh) | 木薯叶脉花叶病毒启动子及其应用 | |
CN1154740C (zh) | 根皮层特异性基因启动子 | |
CN1191565A (zh) | 转录控制序列和方法 | |
US8829170B2 (en) | Construct capable of release in closed circular form from a larger nucleotide sequence permitting site specific expression and/or developmentally regulated expression of selected genetic sequences | |
CN1151183A (zh) | Rps2基因及其应用 | |
US6610907B1 (en) | Cotton leaf curl virus (CLCuV) promoter and its use | |
CN1922327A (zh) | 气孔保卫细胞特异性启动子 | |
CN1161474C (zh) | 基因表达特异性的提高 | |
CN1333833A (zh) | 鉴定非寄主植物抗病基因的新方法 | |
CN111116725B (zh) | 基因Os11g0682000及其编码的蛋白在调控水稻白叶枯病抗性中的应用 | |
CN1809640A (zh) | 延迟植物中种子落粒的方法和手段 | |
CN1793172A (zh) | 无诱导表达基因工程菌株及构建方法和应用 | |
CN1195063C (zh) | 蛋白酶抑制剂融合蛋白 | |
CN1639345A (zh) | 用于表达花组织中转基因的lis启动子 | |
CN108727480B (zh) | 一种转录抑制结构域、其编码基因及其应用 | |
CN1496367A (zh) | 用于在植物中产生番茄黄叶卷缩病毒抗性的材料和方法 | |
CN1353763A (zh) | 来源于鸭跖草黄斑驳病毒和木薯叶脉花叶病毒的嵌合表达启动子 | |
CN1263862C (zh) | 植物启动子和植物终止子 | |
CN1352693A (zh) | 昆虫病毒载体及其应用 | |
CN1234870C (zh) | 新的类似微型反向重复转座元件(mite)的转座元件和转录活化元件 | |
CN1320115C (zh) | 编码海岛棉防卫反应基因的核苷酸序列、含有该序列的植物表达载体及植物细胞 | |
AU2001243939B2 (en) | A construct capable of release in closed circular form from a larger nucleotide sequence permitting site specific expression and/or developmentally regulated expression of selected genetic sequences | |
CN1721536A (zh) | 一种植物抗病相关蛋白及其编码基因与应用 |
Legal Events
Date | Code | Title | Description |
---|---|---|---|
C06 | Publication | ||
PB01 | Publication | ||
C10 | Entry into substantive examination | ||
SE01 | Entry into force of request for substantive examination | ||
ASS | Succession or assignment of patent right |
Owner name: QUT BLUEBOX CO., LTD. Free format text: FORMER OWNER: QUEENSLAND UNIV. OF TECHNOLOGY Effective date: 20110908 Owner name: PHARMAQ BIOLOGICAL INDUSTRY INC. Free format text: FORMER OWNER: QUT BLUEBOX CO., LTD. Effective date: 20110908 |
|
C41 | Transfer of patent application or patent right or utility model | ||
TA01 | Transfer of patent application right |
Effective date of registration: 20110908 Address after: Queensland, Australia Applicant after: Farmac Bio-Industry Co.,Ltd. Address before: Queensland, Australia Applicant before: QUT Blue Box Co.,Ltd. Effective date of registration: 20110908 Address after: Queensland, Australia Applicant after: QUT Blue Box Co.,Ltd. Address before: Queensland, Australia Applicant before: QUEENSLAND University OF TECHNOLOGY |
|
C14 | Grant of patent or utility model | ||
GR01 | Patent grant |