CN1289525C - T2r,一类味觉受体 - Google Patents

T2r,一类味觉受体 Download PDF

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Publication number
CN1289525C
CN1289525C CNB00815435XA CN00815435A CN1289525C CN 1289525 C CN1289525 C CN 1289525C CN B00815435X A CNB00815435X A CN B00815435XA CN 00815435 A CN00815435 A CN 00815435A CN 1289525 C CN1289525 C CN 1289525C
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China
Prior art keywords
seq
taste
cells
protein
amino acid
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Expired - Fee Related
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CNB00815435XA
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English (en)
Chinese (zh)
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CN1387536A (zh
Inventor
C·S·朱克
J·E·阿德莱尔
N·里巴
K·米勒
M·胡恩
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
University of California, San Diego
United States Department of Health and Human Services
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University of California, San Diego
United States Department of Health and Human Services
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Priority claimed from US09/393,634 external-priority patent/US6558910B2/en
Application filed by University of California, San Diego, United States Department of Health and Human Services filed Critical University of California, San Diego
Publication of CN1387536A publication Critical patent/CN1387536A/zh
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Publication of CN1289525C publication Critical patent/CN1289525C/zh
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    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/74—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving hormones or other non-cytokine intercellular protein regulatory factors such as growth factors, including receptors to hormones and growth factors
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/705—Receptors; Cell surface antigens; Cell surface determinants
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/705—Receptors; Cell surface antigens; Cell surface determinants
    • C07K14/72—Receptors; Cell surface antigens; Cell surface determinants for hormones
    • C07K14/723—G protein coupled receptor, e.g. TSHR-thyrotropin-receptor, LH/hCG receptor, FSH receptor
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/02—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving viable microorganisms
    • C12Q1/025—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving viable microorganisms for testing or evaluating the effect of chemical or biological compounds, e.g. drugs, cosmetics
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/5005—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells
    • G01N33/5008—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells for testing or evaluating the effect of chemical or biological compounds, e.g. drugs, cosmetics
    • G01N33/502—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells for testing or evaluating the effect of chemical or biological compounds, e.g. drugs, cosmetics for testing non-proliferative effects
    • G01N33/5041—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells for testing or evaluating the effect of chemical or biological compounds, e.g. drugs, cosmetics for testing non-proliferative effects involving analysis of members of signalling pathways
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/5005—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells
    • G01N33/5008—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells for testing or evaluating the effect of chemical or biological compounds, e.g. drugs, cosmetics
    • G01N33/5044—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells for testing or evaluating the effect of chemical or biological compounds, e.g. drugs, cosmetics involving specific cell types
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/68—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2333/00—Assays involving biological materials from specific organisms or of a specific nature
    • G01N2333/435—Assays involving biological materials from specific organisms or of a specific nature from animals; from humans
    • G01N2333/705—Assays involving receptors, cell surface antigens or cell surface determinants
    • G01N2333/72—Assays involving receptors, cell surface antigens or cell surface determinants for hormones
    • G01N2333/726—G protein coupled receptor, e.g. TSHR-thyrotropin-receptor, LH/hCG receptor, FSH

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  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Immunology (AREA)
  • Molecular Biology (AREA)
  • Biomedical Technology (AREA)
  • Hematology (AREA)
  • Urology & Nephrology (AREA)
  • Cell Biology (AREA)
  • Biochemistry (AREA)
  • Medicinal Chemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Toxicology (AREA)
  • Analytical Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Food Science & Technology (AREA)
  • General Physics & Mathematics (AREA)
  • Pathology (AREA)
  • Zoology (AREA)
  • Genetics & Genomics (AREA)
  • Biophysics (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Wood Science & Technology (AREA)
  • Endocrinology (AREA)
  • General Engineering & Computer Science (AREA)
  • Peptides Or Proteins (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
CNB00815435XA 1999-09-10 2000-09-08 T2r,一类味觉受体 Expired - Fee Related CN1289525C (zh)

Applications Claiming Priority (4)

Application Number Priority Date Filing Date Title
US09/393,634 1999-09-10
US09/393,634 US6558910B2 (en) 1999-09-10 1999-09-10 SF, a novel family of taste receptors
US09/510,332 2000-02-22
US09/510,332 US7244584B2 (en) 1999-09-10 2000-02-22 T2R, a novel family of taste receptors

Related Child Applications (1)

Application Number Title Priority Date Filing Date
CNA2006101598520A Division CN101058605A (zh) 1999-09-10 2000-09-08 T2r，一类味觉受体

Publications (2)

Publication Number Publication Date
CN1387536A CN1387536A (zh) 2002-12-25
CN1289525C true CN1289525C (zh) 2006-12-13

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CNB00815435XA Expired - Fee Related CN1289525C (zh) 1999-09-10 2000-09-08 T2r,一类味觉受体

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Country Link
US (8) US7465550B2 (enExample)
EP (1) EP1214343A2 (enExample)
JP (1) JP4339542B2 (enExample)
CN (1) CN1289525C (enExample)
AU (2) AU773600B2 (enExample)
CA (1) CA2384777A1 (enExample)
IL (1) IL148593A0 (enExample)
MX (1) MXPA02002538A (enExample)
NO (1) NO20021164L (enExample)
WO (1) WO2001018050A2 (enExample)

Families Citing this family (43)

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US6558910B2 (en) 1999-09-10 2003-05-06 The Regents Of The University Of California SF, a novel family of taste receptors
EP1214343A2 (en) 1999-09-10 2002-06-19 The Regents Of The University Of California T2r taste receptor family
TW201006846A (en) 2000-03-07 2010-02-16 Senomyx Inc T1R taste receptor and genes encidung same
CN100447156C (zh) * 2000-04-07 2008-12-31 塞诺米克斯公司 T2r味觉受体及其编码基因
TW201022287A (en) 2001-01-03 2010-06-16 Senomyx Inc T1R taste receptors and genes encoding same
US7301009B2 (en) 2001-06-26 2007-11-27 Senomyx, Inc. Isolated (T1R1/T1R3) umami taste receptors that respond to umami taste stimuli
US20080244761A1 (en) 2001-03-07 2008-10-02 Senomyx, Inc. T1r hetero-oligomeric taste receptors and cell lines that express said receptors and use thereof for identification of taste compounds
US7368285B2 (en) 2001-03-07 2008-05-06 Senomyx, Inc. Heteromeric umami T1R1/T1R3 taste receptors and isolated cells that express same
US8030008B2 (en) 2001-04-05 2011-10-04 Senomyx, Inc. Identification of bitter ligands that specifically activate human T2R receptors and related assays for identifying human bitter taste modulators
US7883856B2 (en) 2001-04-05 2011-02-08 Senomyx Inc. Identification of bitter ligands that specifically activate human T2R receptors and related assays for identifying human bitter taste modulators
US7803982B2 (en) 2001-04-20 2010-09-28 The Mount Sinai School Of Medicine Of New York University T1R3 transgenic animals, cells and related methods
DK2327985T3 (en) 2001-06-26 2016-09-05 Senomyx Inc H1 Oligomeric T1R Taste Receptors and Cell Lines Expressing the Receptors, and Their Use to Identify Taste Compounds
EP1412750B1 (en) 2001-06-26 2012-05-23 Senomyx, Inc. T1r2-t1r3 hetero-oligomeric sweet taste receptors and cell lines that express said receptors and use thereof for identification of sweet taste compounds
IL159640A0 (en) * 2001-07-10 2004-06-01 Senomyx Inc Use of specific t2r taste receptors to identify compounds that block bitter taste
US7314725B2 (en) 2001-07-20 2008-01-01 The United States Of America As Represented By The Secretary Of The Department Of Health And Human Services Phenylthiocarbamide (PTC) taste receptor
EP1442117A4 (en) * 2001-10-12 2005-04-27 Univ California CHEMOLIC RECEPTORS OF THE STOMACH DARM TRAKTS
MXPA04012334A (es) 2002-07-29 2005-07-25 Senomyx Inc Identificacion de un nuevo receptor t2r76 para sabor amargo.
EP2270155A3 (en) * 2002-09-25 2011-08-10 Deutsches Institut für Ernährungsforschung Potsdam-Rehbrücke Bitter taste receptors
EP1581555B1 (en) 2002-12-18 2008-04-30 Givaudan SA Chimeric alpha q-gustducin g-proteins
AU2004258071A1 (en) * 2003-06-19 2005-01-27 The Government Of The United States Of America As Represented By The Secretary Of The Department Of Health And Human Services Variants of human taste receptor genes
WO2006053771A2 (en) 2004-11-18 2006-05-26 Deutsches Institut Für Ernährungsforschung Potsdam-Rehbrücke Stiftung Des Öffentlichen Rechts Agonists of bitter taste receptors and uses thereof
EP1851548A4 (en) 2005-02-08 2010-04-28 Senomyx Inc HUMAN T2R RECEPTORS FOR ACETAMINOPHES, RANITIDINE, STRYCHNIN AND DENATONIUM, AND ASSOCIATED ASSAYS FOR IDENTIFYING MODULATORS BITTER TASTE
US8017336B2 (en) 2005-12-27 2011-09-13 Nihon University Method of examining zinc-deficient taste disturbance by determining expression level of taste receptor genes
US8124360B2 (en) 2006-04-20 2012-02-28 Givaudan S.A. Use of a T1R2 nucleic acid sequence to identify tastants
US20110097741A1 (en) * 2006-04-20 2011-04-28 Jay Patrick Slack Partial t1r2 nucleic acid sequence, receptor protein and its use in screening assays
EP2995950B1 (en) 2006-04-20 2024-01-17 Givaudan SA Method relating to sweetness enhancement
US7811788B2 (en) * 2006-09-05 2010-10-12 Senomyx Inc. Nucleic acid encoding a haplotype of human T2R receptor hT2R50
JP5301464B2 (ja) 2007-01-18 2013-09-25 ジボダン エス エー キメラうま味受容体、それをコードする核酸およびその利用
US8187822B2 (en) 2007-03-30 2012-05-29 Givaudan S.A. Methods to identify modulators
TW200904981A (en) 2007-06-08 2009-02-01 Senomyx Inc Identification of TRPML3 (mcoln3) as a salty taste receptor and use in assays for identifying taste (salty) modulators and/or therapeutics that modulate sodium transport, absorption or excretion and/or aldosterone and/or vasopressin production or release
HUE025137T2 (en) 2007-08-21 2016-01-28 Senomyx Inc Compositions are bitter taste blocking (blocking) compounds
NZ601353A (en) * 2008-02-01 2014-06-27 Chromocell Corp Cell lines and methods for making and using them
US20130115624A1 (en) 2010-05-07 2013-05-09 Givaudan S.A. Methods To Identify Modulators
US20130123135A1 (en) 2010-05-07 2013-05-16 Jay Patrick Slack Methods To Identify Modulators of TAS2R48 Receptors
KR20160074015A (ko) 2010-06-17 2016-06-27 캘리포니아 인스티튜트 오브 테크놀로지 호르몬을 조절하기 위한 방법 및 시스템 및 관련 방법, 작용제 및 조성물
EP2742350B1 (en) 2011-08-08 2019-10-30 The Coca-Cola Company Cell lines comprising endogenous taste receptors and their uses
WO2013159227A1 (en) * 2012-04-25 2013-10-31 University Of Manitoba Bitter taste receptor constitutively active mutants
US9804157B1 (en) 2013-03-15 2017-10-31 Senomyx, Inc. Screening assays to identify compounds which modulate T1R associated taste modalities which eliminate false positives
US9169311B2 (en) 2013-03-15 2015-10-27 Applied Food Biotechnology, Inc. Feline bitter taste receptors and methods
WO2014149829A1 (en) 2013-03-15 2014-09-25 Afb International Feline bitter taste receptors and methods
JP6367315B2 (ja) 2013-05-10 2018-08-01 ペプシコ, インコーポレイテッドPepsiCo Inc. 潜在的味覚調節物質を特定するためのモデルとしての細胞
JP6211685B2 (ja) * 2013-05-10 2017-10-11 ペプシコ, インコーポレイテッドPepsiCo Inc. 味覚受容体インターナリゼーションアッセイ
ES2802376T3 (es) 2014-02-12 2021-01-19 Firmenich Incorporated Procedimiento mejorado para la síntesis de 1-bencil-3-(1-(isoxazol-4-ilmetil)-1h-pirazol-4-il)imidazolidin-2,4-dionas sustituidas

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AU3665893A (en) 1992-02-12 1993-09-03 United States Of America, As Represented By The Secretary, Department Of Health And Human Services, The Sequences characteristic of human gene transcription product
DE69731655T2 (de) 1996-09-04 2005-11-24 Syngenta Mogen B.V. Gegen pilze gerichtete proteine, dafuer kodierende dna und wirtsorganismen, die diese beinhalten
AU2685599A (en) 1998-02-18 1999-09-06 Genetics Institute Inc. Secreted proteins and polynucleotides encoding them
AU2378600A (en) 1998-12-23 2000-07-31 Mount Sinai School Of Medicine Of The City University Of New York, The Inhibitors of the bitter taste response
US6558910B2 (en) 1999-09-10 2003-05-06 The Regents Of The University Of California SF, a novel family of taste receptors
EP1214343A2 (en) 1999-09-10 2002-06-19 The Regents Of The University Of California T2r taste receptor family

Also Published As

Publication number Publication date
MXPA02002538A (es) 2005-07-01
NO20021164D0 (no) 2002-03-08
US20140120553A1 (en) 2014-05-01
EP1214343A2 (en) 2002-06-19
US7595166B2 (en) 2009-09-29
AU7366400A (en) 2001-04-10
US20080108088A1 (en) 2008-05-08
US9817000B2 (en) 2017-11-14
AU773600B2 (en) 2004-05-27
US20120009598A1 (en) 2012-01-12
WO2001018050A3 (en) 2002-01-10
WO2001018050A2 (en) 2001-03-15
IL148593A0 (en) 2002-09-12
US9063124B2 (en) 2015-06-23
US8329885B2 (en) 2012-12-11
NO20021164L (no) 2002-05-10
US20050048586A1 (en) 2005-03-03
CN1387536A (zh) 2002-12-25
JP4339542B2 (ja) 2009-10-07
US20150260733A1 (en) 2015-09-17
US20180128842A1 (en) 2018-05-10
US20100062456A1 (en) 2010-03-11
US20130189712A1 (en) 2013-07-25
US7868150B2 (en) 2011-01-11
JP2003510037A (ja) 2003-03-18
US8624012B2 (en) 2014-01-07
WO2001018050A9 (en) 2002-02-07
AU2004203826A1 (en) 2004-09-16
US7465550B2 (en) 2008-12-16
CA2384777A1 (en) 2001-03-15

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