CN1289525C - T2r,一类味觉受体 - Google Patents

T2r,一类味觉受体 Download PDF

Info

Publication number
CN1289525C
CN1289525C CNB00815435XA CN00815435A CN1289525C CN 1289525 C CN1289525 C CN 1289525C CN B00815435X A CNB00815435X A CN B00815435XA CN 00815435 A CN00815435 A CN 00815435A CN 1289525 C CN1289525 C CN 1289525C
Authority
CN
China
Prior art keywords
seq
taste
cells
protein
amino acid
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Fee Related
Application number
CNB00815435XA
Other languages
English (en)
Chinese (zh)
Other versions
CN1387536A (zh
Inventor
C·S·朱克
J·E·阿德莱尔
N·里巴
K·米勒
M·胡恩
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
University of California San Diego UCSD
US Department of Health and Human Services
Original Assignee
University of California San Diego UCSD
US Department of Health and Human Services
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from US09/393,634 external-priority patent/US6558910B2/en
Application filed by University of California San Diego UCSD, US Department of Health and Human Services filed Critical University of California San Diego UCSD
Publication of CN1387536A publication Critical patent/CN1387536A/zh
Application granted granted Critical
Publication of CN1289525C publication Critical patent/CN1289525C/zh
Anticipated expiration legal-status Critical
Expired - Fee Related legal-status Critical Current

Links

Images

Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/74—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving hormones or other non-cytokine intercellular protein regulatory factors such as growth factors, including receptors to hormones and growth factors
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/705—Receptors; Cell surface antigens; Cell surface determinants
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/705—Receptors; Cell surface antigens; Cell surface determinants
    • C07K14/72—Receptors; Cell surface antigens; Cell surface determinants for hormones
    • C07K14/723—G protein coupled receptor, e.g. TSHR-thyrotropin-receptor, LH/hCG receptor, FSH receptor
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/02—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving viable microorganisms
    • C12Q1/025—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving viable microorganisms for testing or evaluating the effect of chemical or biological compounds, e.g. drugs, cosmetics
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/5005—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells
    • G01N33/5008—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells for testing or evaluating the effect of chemical or biological compounds, e.g. drugs, cosmetics
    • G01N33/502—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells for testing or evaluating the effect of chemical or biological compounds, e.g. drugs, cosmetics for testing non-proliferative effects
    • G01N33/5041—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells for testing or evaluating the effect of chemical or biological compounds, e.g. drugs, cosmetics for testing non-proliferative effects involving analysis of members of signalling pathways
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/5005—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells
    • G01N33/5008—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells for testing or evaluating the effect of chemical or biological compounds, e.g. drugs, cosmetics
    • G01N33/5044—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells for testing or evaluating the effect of chemical or biological compounds, e.g. drugs, cosmetics involving specific cell types
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/68—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2333/00—Assays involving biological materials from specific organisms or of a specific nature
    • G01N2333/435—Assays involving biological materials from specific organisms or of a specific nature from animals; from humans
    • G01N2333/705—Assays involving receptors, cell surface antigens or cell surface determinants
    • G01N2333/72—Assays involving receptors, cell surface antigens or cell surface determinants for hormones
    • G01N2333/726—G protein coupled receptor, e.g. TSHR-thyrotropin-receptor, LH/hCG receptor, FSH

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Immunology (AREA)
  • Molecular Biology (AREA)
  • Biomedical Technology (AREA)
  • Hematology (AREA)
  • Urology & Nephrology (AREA)
  • Cell Biology (AREA)
  • Medicinal Chemistry (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Toxicology (AREA)
  • Analytical Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Food Science & Technology (AREA)
  • General Physics & Mathematics (AREA)
  • Pathology (AREA)
  • Zoology (AREA)
  • Genetics & Genomics (AREA)
  • Biophysics (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Wood Science & Technology (AREA)
  • Endocrinology (AREA)
  • General Engineering & Computer Science (AREA)
  • Peptides Or Proteins (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
CNB00815435XA 1999-09-10 2000-09-08 T2r,一类味觉受体 Expired - Fee Related CN1289525C (zh)

Applications Claiming Priority (4)

Application Number Priority Date Filing Date Title
US09/393,634 US6558910B2 (en) 1999-09-10 1999-09-10 SF, a novel family of taste receptors
US09/393,634 1999-09-10
US09/510,332 2000-02-22
US09/510,332 US7244584B2 (en) 1999-09-10 2000-02-22 T2R, a novel family of taste receptors

Related Child Applications (1)

Application Number Title Priority Date Filing Date
CNA2006101598520A Division CN101058605A (zh) 1999-09-10 2000-09-08 T2r，一类味觉受体

Publications (2)

Publication Number Publication Date
CN1387536A CN1387536A (zh) 2002-12-25
CN1289525C true CN1289525C (zh) 2006-12-13

Family

ID=27014384

Family Applications (1)

Application Number Title Priority Date Filing Date
CNB00815435XA Expired - Fee Related CN1289525C (zh) 1999-09-10 2000-09-08 T2r,一类味觉受体

Country Status (10)

Country Link
US (8) US7465550B2 (enExample)
EP (1) EP1214343A2 (enExample)
JP (1) JP4339542B2 (enExample)
CN (1) CN1289525C (enExample)
AU (2) AU773600B2 (enExample)
CA (1) CA2384777A1 (enExample)
IL (1) IL148593A0 (enExample)
MX (1) MXPA02002538A (enExample)
NO (1) NO20021164L (enExample)
WO (1) WO2001018050A2 (enExample)

Families Citing this family (43)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6558910B2 (en) 1999-09-10 2003-05-06 The Regents Of The University Of California SF, a novel family of taste receptors
WO2001018050A2 (en) 1999-09-10 2001-03-15 The Regents Of The University Of California T2r taste receptor family
TW201006846A (en) * 2000-03-07 2010-02-16 Senomyx Inc T1R taste receptor and genes encidung same
AU2001251258B2 (en) * 2000-04-07 2008-06-05 Senomyx, Inc. T2r taste receptors and genes encoding same
TW201022287A (en) 2001-01-03 2010-06-16 Senomyx Inc T1R taste receptors and genes encoding same
US20080244761A1 (en) 2001-03-07 2008-10-02 Senomyx, Inc. T1r hetero-oligomeric taste receptors and cell lines that express said receptors and use thereof for identification of taste compounds
US7368285B2 (en) 2001-03-07 2008-05-06 Senomyx, Inc. Heteromeric umami T1R1/T1R3 taste receptors and isolated cells that express same
US7301009B2 (en) 2001-06-26 2007-11-27 Senomyx, Inc. Isolated (T1R1/T1R3) umami taste receptors that respond to umami taste stimuli
US7883856B2 (en) 2001-04-05 2011-02-08 Senomyx Inc. Identification of bitter ligands that specifically activate human T2R receptors and related assays for identifying human bitter taste modulators
US8030008B2 (en) 2001-04-05 2011-10-04 Senomyx, Inc. Identification of bitter ligands that specifically activate human T2R receptors and related assays for identifying human bitter taste modulators
US7803982B2 (en) 2001-04-20 2010-09-28 The Mount Sinai School Of Medicine Of New York University T1R3 transgenic animals, cells and related methods
EP2327985B2 (en) 2001-06-26 2019-08-21 Senomyx, Inc. T1R Hetero-oligomeric taste receptors and cell lines that express said receptors and use thereof for identification of taste compounds
DK2327985T3 (en) 2001-06-26 2016-09-05 Senomyx Inc H1 Oligomeric T1R Taste Receptors and Cell Lines Expressing the Receptors, and Their Use to Identify Taste Compounds
EP1502112A4 (en) 2001-07-10 2008-07-23 Senomyx Inc USE OF SPECIFIC T2R TASTE RECIPES TO IDENTIFY CONNECTIONS WHICH BLOCK A BITTER TASTE
EP1523308A4 (en) 2001-07-20 2007-01-10 Us Gov Health & Human Serv PHENYL THIOCARBAMIDE (PTC) TREATMENT RECEPTOR
EP1442117A4 (en) * 2001-10-12 2005-04-27 Univ California CHEMOLIC RECEPTORS OF THE STOMACH DARM TRAKTS
CA2488863A1 (en) 2002-07-29 2004-02-05 Senomyx, Inc. Identification of a novel bitter taste receptor, t2r76
EP2163623A3 (en) * 2002-09-25 2010-05-26 Deutsches Institut für Ernährungsforschung Potsdam-Rehbrücke Bitter taste receptors
WO2004055048A2 (en) 2002-12-18 2004-07-01 Givaudan Sa Chimeric alpha q-gustducin g-proteins
WO2005007891A2 (en) * 2003-06-19 2005-01-27 The Government Of The United States Of America, Represented By The Secretary Of The Department Of Health And Human Services Variants of human taste receptor genes
US7829299B2 (en) 2004-11-18 2010-11-09 Deutsches Institut fur Ernahrungforschung Potsdam-Rehbrucke Stiftung des Offentlichen Rechts Agonists of bitter taste receptors and uses thereof
CA2597085A1 (en) * 2005-02-08 2006-08-17 Senomyx, Inc. Phuman t2r receptors for acetaminophen, ranitidine, strychnine and denatonium and related assays for identifying human bitter taste modulators
WO2007074937A1 (ja) 2005-12-27 2007-07-05 Nihon University 亜鉛欠乏性味覚異常検査法
US20110097741A1 (en) * 2006-04-20 2011-04-28 Jay Patrick Slack Partial t1r2 nucleic acid sequence, receptor protein and its use in screening assays
JP2009533065A (ja) 2006-04-20 2009-09-17 ジボダン エス エー 味覚物質同定のための機能的方法
EP2047254B1 (en) 2006-04-20 2015-10-28 Givaudan SA Method relating to sweetness enhancement
JP2010514411A (ja) * 2006-09-05 2010-05-06 セノミクス・インコーポレーテッド ヒトＴ２Ｒ受容体ｈＴ２Ｒ５０の新規ハプロタイプおよびヒト苦味モジュレータを同定するためのアッセイにおけるその使用
KR101511394B1 (ko) 2007-01-18 2015-04-10 지보당 에스아 키메라 우마미 미각 수용체, 이를 코딩하는 핵산 및 이의 용도
EP2137322B1 (en) 2007-03-30 2013-02-27 Givaudan SA Methods to identify tas2r modulators
EP2164976A4 (en) 2007-06-08 2010-06-30 Senomyx Inc THEORETICAL BASIS, METHODS AND TESTS FOR IDENTIFYING FLAVOR SPECIES OF HUMAN AND NON-MUSCLE PRIMATES AND USE THEREOF IN TASTE MODULATOR AND THERAPEUTIC SCREENING TESTS
WO2009025793A2 (en) 2007-08-21 2009-02-26 Senomyx, Inc. Human t2r bitterness receptors and uses thereof
CA2713885A1 (en) * 2008-02-01 2009-08-20 Chromocell Corporation Novel cell lines and methods
US20130115624A1 (en) 2010-05-07 2013-05-09 Givaudan S.A. Methods To Identify Modulators
EP2567231A1 (en) 2010-05-07 2013-03-13 Givaudan SA Methods to identify modulators of tas2r4s receptors
WO2011160093A2 (en) * 2010-06-17 2011-12-22 California Institute Of Technology Methods and systems for modulating hormones and related methods, agents and compositions
WO2013022948A1 (en) 2011-08-08 2013-02-14 The Coca-Cola Company Cell lines comprising endogenous taste receptors and their uses
WO2013159227A1 (en) * 2012-04-25 2013-10-31 University Of Manitoba Bitter taste receptor constitutively active mutants
US9169311B2 (en) 2013-03-15 2015-10-27 Applied Food Biotechnology, Inc. Feline bitter taste receptors and methods
US9804157B1 (en) 2013-03-15 2017-10-31 Senomyx, Inc. Screening assays to identify compounds which modulate T1R associated taste modalities which eliminate false positives
ES2797547T3 (es) 2013-03-15 2020-12-02 Applied Food Biotech Inc Receptores del sabor amargo felino y métodos.
US10107794B2 (en) 2013-05-10 2018-10-23 Pepsico, Inc. Methods of using U2-OS cells to identify potential sweet taste modulators
MX384586B (es) * 2013-05-10 2025-03-14 Pepsico Inc Ensayo de internalización del receptor del gusto.
ES2802376T3 (es) 2014-02-12 2021-01-19 Firmenich Incorporated Procedimiento mejorado para la síntesis de 1-bencil-3-(1-(isoxazol-4-ilmetil)-1h-pirazol-4-il)imidazolidin-2,4-dionas sustituidas

Family Cites Families (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1993016178A2 (en) 1992-02-12 1993-08-19 The United States Of America, As Represented By The Secretary, Department Of Health And Human Services Sequences characteristic of human gene transcription product
CN1155714C (zh) 1996-09-04 2004-06-30 辛根塔默根有限公司 抗真菌蛋白及其编码dna和掺入此dna的宿主
AU2685599A (en) 1998-02-18 1999-09-06 Genetics Institute Inc. Secreted proteins and polynucleotides encoding them
WO2000038536A2 (en) 1998-12-23 2000-07-06 Mount Sinai School Of Medicine Of New York University Inhibitors of the bitter taste response
US6558910B2 (en) 1999-09-10 2003-05-06 The Regents Of The University Of California SF, a novel family of taste receptors
WO2001018050A2 (en) 1999-09-10 2001-03-15 The Regents Of The University Of California T2r taste receptor family

Also Published As

Publication number Publication date
US20130189712A1 (en) 2013-07-25
US20080108088A1 (en) 2008-05-08
AU2004203826A1 (en) 2004-09-16
US8624012B2 (en) 2014-01-07
EP1214343A2 (en) 2002-06-19
MXPA02002538A (es) 2005-07-01
CN1387536A (zh) 2002-12-25
WO2001018050A2 (en) 2001-03-15
US20120009598A1 (en) 2012-01-12
AU773600B2 (en) 2004-05-27
CA2384777A1 (en) 2001-03-15
US7868150B2 (en) 2011-01-11
WO2001018050A9 (en) 2002-02-07
AU7366400A (en) 2001-04-10
US20050048586A1 (en) 2005-03-03
US7595166B2 (en) 2009-09-29
US9063124B2 (en) 2015-06-23
NO20021164D0 (no) 2002-03-08
NO20021164L (no) 2002-05-10
IL148593A0 (en) 2002-09-12
JP2003510037A (ja) 2003-03-18
WO2001018050A3 (en) 2002-01-10
US20100062456A1 (en) 2010-03-11
US8329885B2 (en) 2012-12-11
US9817000B2 (en) 2017-11-14
JP4339542B2 (ja) 2009-10-07
US20140120553A1 (en) 2014-05-01
US20150260733A1 (en) 2015-09-17
US20180128842A1 (en) 2018-05-10
US7465550B2 (en) 2008-12-16

Similar Documents

Publication Publication Date Title
CN1289525C (zh) T2r,一类味觉受体
CN101058605A (zh) T2r，一类味觉受体
US20040214239A1 (en) Functional coupling of T1Rs and T2Rs by GI proteins, and cell-based assays for the identification of T1R and T2R modulators
CN1178952C (zh) 编码与感觉转导有关的g-蛋白偶联受体的核酸
JP2013247957A (ja) Ｔ１ｒ味覚受容体とこれをコードする遺伝子
CN1317010A (zh) 编码与感觉转导有关的g－蛋白偶联受体的核酸
JP4324474B2 (ja) Ｇタンパク質共役受容体媒介活性の新規細胞系アッセイ
JP2011067208A (ja) Ａｐｊ受容体リガンドを同定するための試験システム
US6358695B1 (en) Methods of screening for agonists and antagonists of the HNEAA81 receptor
WO2002099381A2 (en) A single-cell biosensor for the measurement of gpcr ligands in a test sample
EP2483696B1 (en) Natural peptide and derivatives as modulators of GPCR GPR1 and uses thereof
US6461836B1 (en) Molecular cloning of a 7TM receptor (AxOR34) and screening methods thereof
EP1137938A1 (en) Methods of screening for agonists and antagonists of the hdpxu17 receptor
EP2149606B1 (en) Nucleic acid molecules and methods for identifying modulators of GPR84 activity
WO2007100618A2 (en) Novel cell-based phosphodiesterase assays
EP1692171B1 (en) Agents modulating the activity of ccrl2 and methods of using the same
KR101535262B1 (ko) 인간 고아 ｇ-단백질 결합 수용체 단백질의 효능제 및 저해제 탐색을 위한 ｈｔｓ 시스템
JP2005000132A (ja) Ｇα蛋白質共発現によるＧＰＣＲ計測方法
WO2000031107A1 (en) 7tm receptor hlwar77
US7067277B1 (en) Chimeric G proteins and uses thereof
US20010021509A1 (en) cDNA clone HNEAA81 that encodes a human 7-transmembrane receptor
WO2009124776A1 (en) A test system for detecting intracellular cyclic nucleotide level and methods and uses thereof
KR20100067849A (ko) 도파민 ｄ1 수용체 발현 세포주 및 이를 이용한 신약 개발 방법
Takeda et al. Screening of Ligands for Human GPCRs by the Use of Receptor-Gα Fusion Proteins
JPWO2003027142A1 (ja) 新規ｇ蛋白質共役型受容体

Legal Events

Date Code Title Description
C06 Publication
PB01 Publication
C10 Entry into substantive examination
SE01 Entry into force of request for substantive examination
C14 Grant of patent or utility model
GR01 Patent grant
CF01 Termination of patent right due to non-payment of annual fee

Granted publication date: 20061213

Termination date: 20140908

EXPY Termination of patent right or utility model