CN1249784A - 一种体外构建dna文库的方法 - Google Patents

一种体外构建dna文库的方法 Download PDF

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Publication number
CN1249784A
CN1249784A CN98802958A CN98802958A CN1249784A CN 1249784 A CN1249784 A CN 1249784A CN 98802958 A CN98802958 A CN 98802958A CN 98802958 A CN98802958 A CN 98802958A CN 1249784 A CN1249784 A CN 1249784A
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CN
China
Prior art keywords
template
polymerase
dna
primers
primer
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CN98802958A
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English (en)
Chinese (zh)
Inventor
杰斯珀·文德
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Novo Nordisk AS
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Novo Nordisk AS
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1034—Isolating an individual clone by screening libraries
    • C12N15/1093—General methods of preparing gene libraries, not provided for in other subgroups
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/102—Mutagenizing nucleic acids
    • C12N15/1027—Mutagenizing nucleic acids by DNA shuffling, e.g. RSR, STEP, RPR
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6811—Selection methods for production or design of target specific oligonucleotides or binding molecules

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  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Molecular Biology (AREA)
  • Biophysics (AREA)
  • Biomedical Technology (AREA)
  • Physics & Mathematics (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Analytical Chemistry (AREA)
  • Plant Pathology (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Immunology (AREA)
  • Bioinformatics & Computational Biology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Saccharide Compounds (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
  • Enzymes And Modification Thereof (AREA)
CN98802958A 1997-03-18 1998-03-18 一种体外构建dna文库的方法 Pending CN1249784A (zh)

Applications Claiming Priority (6)

Application Number Priority Date Filing Date Title
DK30797 1997-03-18
DK0307/1997 1997-03-18
DK43497 1997-04-17
DK0434/1997 1997-04-17
DK62597 1997-05-30
DK0625/1997 1997-05-30

Publications (1)

Publication Number Publication Date
CN1249784A true CN1249784A (zh) 2000-04-05

Family

ID=27220576

Family Applications (1)

Application Number Title Priority Date Filing Date
CN98802958A Pending CN1249784A (zh) 1997-03-18 1998-03-18 一种体外构建dna文库的方法

Country Status (14)

Country Link
EP (1) EP0973940B1 (https=)
JP (1) JP2001515356A (https=)
KR (1) KR20000076363A (https=)
CN (1) CN1249784A (https=)
AT (1) ATE399880T1 (https=)
AU (1) AU741834B2 (https=)
BR (1) BR9808368A (https=)
CA (1) CA2284253A1 (https=)
DE (1) DE69839668D1 (https=)
DK (1) DK0973940T3 (https=)
IL (1) IL131966A0 (https=)
NZ (1) NZ337900A (https=)
PL (1) PL335860A1 (https=)
WO (1) WO1998041653A1 (https=)

Cited By (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN103539744A (zh) * 2013-10-17 2014-01-29 北京颖泰嘉和生物科技有限公司 一种制备3-(3,5-二氯苯基)-2,4-咪唑烷二酮的方法
WO2016134521A1 (en) * 2015-02-27 2016-09-01 The University Of Hong Kong Dna display and methods thereof
CN108588050A (zh) * 2018-05-14 2018-09-28 北京艾克伦医疗科技有限公司 Dna聚合酶以及核酸检测方法和试剂盒
CN114197061A (zh) * 2022-02-17 2022-03-18 北京贝瑞和康生物技术有限公司 用于构建检测染色体拷贝数变异的测序文库的方法和试剂盒
CN117157148A (zh) * 2021-02-08 2023-12-01 胡桃夹子治疗公司 制备合成模板的方法

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