CN1210557A - 用于在细胞中体内产生突变体文库方法 - Google Patents

用于在细胞中体内产生突变体文库方法 Download PDF

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Publication number
CN1210557A
CN1210557A CN97191974A CN97191974A CN1210557A CN 1210557 A CN1210557 A CN 1210557A CN 97191974 A CN97191974 A CN 97191974A CN 97191974 A CN97191974 A CN 97191974A CN 1210557 A CN1210557 A CN 1210557A
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China
Prior art keywords
polypeptide
cells
interest
dna
variant
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CN97191974A
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English (en)
Chinese (zh)
Inventor
T·V·伯彻特
S·D·埃尔利克
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Novo Nordisk AS
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Novo Nordisk AS
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Publication of CN1210557A publication Critical patent/CN1210557A/zh
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2521/00—Reaction characterised by the enzymatic activity
    • C12Q2521/10—Nucleotidyl transfering
    • C12Q2521/101—DNA polymerase

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  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Biotechnology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • General Engineering & Computer Science (AREA)
  • Wood Science & Technology (AREA)
  • Biomedical Technology (AREA)
  • Microbiology (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Plant Pathology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
CN97191974A 1996-01-10 1997-01-10 用于在细胞中体内产生突变体文库方法 Pending CN1210557A (zh)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
DK0018/96 1996-01-10
DK1896 1996-01-10

Related Child Applications (1)

Application Number Title Priority Date Filing Date
CNA2006101101626A Division CN101070537A (zh) 1996-01-10 1997-01-10 用于在细胞中体内产生突变体文库方法

Publications (1)

Publication Number Publication Date
CN1210557A true CN1210557A (zh) 1999-03-10

Family

ID=8088824

Family Applications (2)

Application Number Title Priority Date Filing Date
CN97191974A Pending CN1210557A (zh) 1996-01-10 1997-01-10 用于在细胞中体内产生突变体文库方法
CNA2006101101626A Pending CN101070537A (zh) 1996-01-10 1997-01-10 用于在细胞中体内产生突变体文库方法

Family Applications After (1)

Application Number Title Priority Date Filing Date
CNA2006101101626A Pending CN101070537A (zh) 1996-01-10 1997-01-10 用于在细胞中体内产生突变体文库方法

Country Status (8)

Country Link
EP (1) EP0873398B1 (enExample)
JP (1) JP2000502568A (enExample)
CN (2) CN1210557A (enExample)
AT (1) ATE293684T1 (enExample)
AU (1) AU1367897A (enExample)
DE (1) DE69733059T2 (enExample)
DK (1) DK0873398T3 (enExample)
WO (1) WO1997025410A1 (enExample)

Cited By (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN101532181B (zh) * 2009-03-13 2011-07-20 南京仙奕基因科技有限公司 原位构建基因突变文库的方法及试剂盒
CN105283588A (zh) * 2013-02-26 2016-01-27 艾希奥美公司 用于产生用于定向进化的文库的方法
CN108342407A (zh) * 2018-03-07 2018-07-31 江南大学 一种得到高表达、高活力及高稳定性的酶突变体的方法

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US5837458A (en) 1994-02-17 1998-11-17 Maxygen, Inc. Methods and compositions for cellular and metabolic engineering
US20060257890A1 (en) 1996-05-20 2006-11-16 Maxygen, Inc. Methods and compositions for cellular and metabolic engineering
US6995017B1 (en) 1994-02-17 2006-02-07 Maxygen, Inc. Methods for generating polynucleotides having desired characteristics by iterative selection and recombination
US6165793A (en) * 1996-03-25 2000-12-26 Maxygen, Inc. Methods for generating polynucleotides having desired characteristics by iterative selection and recombination
US6309883B1 (en) 1994-02-17 2001-10-30 Maxygen, Inc. Methods and compositions for cellular and metabolic engineering
US6335160B1 (en) 1995-02-17 2002-01-01 Maxygen, Inc. Methods and compositions for polypeptide engineering
US5605793A (en) 1994-02-17 1997-02-25 Affymax Technologies N.V. Methods for in vitro recombination
US6117679A (en) 1994-02-17 2000-09-12 Maxygen, Inc. Methods for generating polynucleotides having desired characteristics by iterative selection and recombination
US6326204B1 (en) 1997-01-17 2001-12-04 Maxygen, Inc. Evolution of whole cells and organisms by recursive sequence recombination
US7148054B2 (en) 1997-01-17 2006-12-12 Maxygen, Inc. Evolution of whole cells and organisms by recursive sequence recombination
EP2261373A3 (en) 1997-01-17 2011-12-14 Codexis Mayflower Holdings, LLC Evolution of whole cells and organisms by recursive sequence recombination
US6153410A (en) * 1997-03-25 2000-11-28 California Institute Of Technology Recombination of polynucleotide sequences using random or defined primers
WO1999029902A1 (en) 1997-12-08 1999-06-17 California Institute Of Technology Method for creating polynucleotide and polypeptide sequences
DK1042461T3 (da) * 1997-12-22 2007-09-24 Dsm Ip Assets Bv Ekspressionskloning i filiforme svampe
US7390619B1 (en) 1998-02-11 2008-06-24 Maxygen, Inc. Optimization of immunomodulatory properties of genetic vaccines
CA2325567A1 (en) 1998-05-01 1999-11-11 Maxygen, Inc. Optimization of pest resistance genes using dna shuffling
WO2000009682A1 (en) 1998-08-12 2000-02-24 Maxygen, Inc. Dna shuffling of monooxygenase genes for production of industrial chemicals
WO2000020573A2 (en) 1998-10-07 2000-04-13 Maxygen, Inc. Dna shuffling to produce nucleic acids for mycotoxin detoxification
EP1129184A1 (en) 1998-11-10 2001-09-05 Maxygen, Inc. Modified adp-glucose pyrophosphorylase for improvement and optimation of plant phenotypes
US6436675B1 (en) 1999-09-28 2002-08-20 Maxygen, Inc. Use of codon-varied oligonucleotide synthesis for synthetic shuffling
WO2000042560A2 (en) 1999-01-19 2000-07-20 Maxygen, Inc. Methods for making character strings, polynucleotides and polypeptides
US6376246B1 (en) 1999-02-05 2002-04-23 Maxygen, Inc. Oligonucleotide mediated nucleic acid recombination
US6917882B2 (en) 1999-01-19 2005-07-12 Maxygen, Inc. Methods for making character strings, polynucleotides and polypeptides having desired characteristics
US7024312B1 (en) 1999-01-19 2006-04-04 Maxygen, Inc. Methods for making character strings, polynucleotides and polypeptides having desired characteristics
US6961664B2 (en) 1999-01-19 2005-11-01 Maxygen Methods of populating data structures for use in evolutionary simulations
IL144657A0 (en) 1999-02-11 2002-06-30 Maxygen Inc High throughput mass spectrometry
US6531316B1 (en) 1999-03-05 2003-03-11 Maxyag, Inc. Encryption of traits using split gene sequences and engineered genetic elements
US6365377B1 (en) 1999-03-05 2002-04-02 Maxygen, Inc. Recombination of insertion modified nucleic acids
US7430477B2 (en) 1999-10-12 2008-09-30 Maxygen, Inc. Methods of populating data structures for use in evolutionary simulations
US6686515B1 (en) 1999-11-23 2004-02-03 Maxygen, Inc. Homologous recombination in plants
US7115712B1 (en) 1999-12-02 2006-10-03 Maxygen, Inc. Cytokine polypeptides
IL150291A0 (en) 2000-01-11 2002-12-01 Maxygen Inc Integrated systems and methods for diversity generation and screening
AU2001268716A1 (en) 2000-06-23 2002-01-08 Maxygen, Inc. Novel chimeric promoters
AU2001272978A1 (en) 2000-06-23 2002-01-08 Maxygen, Inc. Novel co-stimulatory molecules
US6858422B2 (en) 2000-07-13 2005-02-22 Codexis, Inc. Lipase genes
JP4647117B2 (ja) * 2001-03-07 2011-03-09 セレスター・レキシコ・サイエンシズ株式会社 突然変異誘発方法
AU2003258658A1 (en) * 2002-08-29 2004-03-19 Andreas Beck Improved dna and proteins
DE102005037351B3 (de) * 2005-08-08 2007-01-11 Geneart Ag Verfahren für die kontinuierliche zielgerichtete Evolution von Proteinen in vitro
US20090269803A1 (en) * 2005-09-21 2009-10-29 Andreas Meyer In Vivo Generation of Dna, Rna, Peptide, and Protein Libraries
KR101098032B1 (ko) * 2008-04-22 2011-12-23 한국생명공학연구원 4차 연속 피씨알, 4차 블록 피씨알, 또는 유전자 합성방법을 이용한 균주 특이적 바코드를 포함하는 유전자 적중 이형접합체 분열효모 균주의 제조방법
CN111574633B (zh) * 2020-05-15 2022-04-08 清华大学 一种具有广谱抗病毒功能的蛋白质CbAE的应用
CN114921440B (zh) * 2022-05-16 2023-05-05 北京昕大洋科技发展有限公司 一种木聚糖酶突变体及其应用

Family Cites Families (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
FR2641793B1 (fr) * 1988-12-26 1993-10-01 Setratech Procede de recombinaison in vivo de sequences d'adn presentant des mesappariements de bases

Cited By (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN101532181B (zh) * 2009-03-13 2011-07-20 南京仙奕基因科技有限公司 原位构建基因突变文库的方法及试剂盒
CN105283588A (zh) * 2013-02-26 2016-01-27 艾希奥美公司 用于产生用于定向进化的文库的方法
US10301617B2 (en) 2013-02-26 2019-05-28 Axiomx, Inc. Methods for the production of libraries for directed evolution
US11066662B2 (en) 2013-02-26 2021-07-20 Axiomx, Inc. Methods for the production of libraries for directed evolution
CN108342407A (zh) * 2018-03-07 2018-07-31 江南大学 一种得到高表达、高活力及高稳定性的酶突变体的方法

Also Published As

Publication number Publication date
DK0873398T3 (da) 2005-08-15
DE69733059T2 (de) 2006-03-02
EP0873398B1 (en) 2005-04-20
ATE293684T1 (de) 2005-05-15
WO1997025410A1 (en) 1997-07-17
JP2000502568A (ja) 2000-03-07
AU1367897A (en) 1997-08-01
EP0873398A1 (en) 1998-10-28
CN101070537A (zh) 2007-11-14
DE69733059D1 (de) 2005-05-25

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