AU1367897A - A method for in vivo production of a mutant library in cells - Google Patents

A method for in vivo production of a mutant library in cells

Info

Publication number
AU1367897A
AU1367897A AU13678/97A AU1367897A AU1367897A AU 1367897 A AU1367897 A AU 1367897A AU 13678/97 A AU13678/97 A AU 13678/97A AU 1367897 A AU1367897 A AU 1367897A AU 1367897 A AU1367897 A AU 1367897A
Authority
AU
Australia
Prior art keywords
cells
replication
vivo production
genetic
polypeptide
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Abandoned
Application number
AU13678/97A
Other languages
English (en)
Inventor
Torben Vedel Borchert
Stanislav Dusko Ehrlich
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Novo Nordisk AS
Original Assignee
Novo Nordisk AS
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Novo Nordisk AS filed Critical Novo Nordisk AS
Publication of AU1367897A publication Critical patent/AU1367897A/en
Abandoned legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2521/00—Reaction characterised by the enzymatic activity
    • C12Q2521/10—Nucleotidyl transfering
    • C12Q2521/101—DNA polymerase

Landscapes

  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Biotechnology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • General Engineering & Computer Science (AREA)
  • Wood Science & Technology (AREA)
  • Biomedical Technology (AREA)
  • Microbiology (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Plant Pathology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
AU13678/97A 1996-01-10 1997-01-10 A method for in vivo production of a mutant library in cells Abandoned AU1367897A (en)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
DK18/96 1996-01-10
DK1896 1996-01-10
PCT/DK1997/000014 WO1997025410A1 (en) 1996-01-10 1997-01-10 A method for in vivo production of a mutant library in cells

Publications (1)

Publication Number Publication Date
AU1367897A true AU1367897A (en) 1997-08-01

Family

ID=8088824

Family Applications (1)

Application Number Title Priority Date Filing Date
AU13678/97A Abandoned AU1367897A (en) 1996-01-10 1997-01-10 A method for in vivo production of a mutant library in cells

Country Status (8)

Country Link
EP (1) EP0873398B1 (enExample)
JP (1) JP2000502568A (enExample)
CN (2) CN1210557A (enExample)
AT (1) ATE293684T1 (enExample)
AU (1) AU1367897A (enExample)
DE (1) DE69733059T2 (enExample)
DK (1) DK0873398T3 (enExample)
WO (1) WO1997025410A1 (enExample)

Families Citing this family (45)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20060257890A1 (en) 1996-05-20 2006-11-16 Maxygen, Inc. Methods and compositions for cellular and metabolic engineering
US6117679A (en) 1994-02-17 2000-09-12 Maxygen, Inc. Methods for generating polynucleotides having desired characteristics by iterative selection and recombination
US5605793A (en) 1994-02-17 1997-02-25 Affymax Technologies N.V. Methods for in vitro recombination
US6995017B1 (en) 1994-02-17 2006-02-07 Maxygen, Inc. Methods for generating polynucleotides having desired characteristics by iterative selection and recombination
US5837458A (en) 1994-02-17 1998-11-17 Maxygen, Inc. Methods and compositions for cellular and metabolic engineering
US6335160B1 (en) 1995-02-17 2002-01-01 Maxygen, Inc. Methods and compositions for polypeptide engineering
US6165793A (en) * 1996-03-25 2000-12-26 Maxygen, Inc. Methods for generating polynucleotides having desired characteristics by iterative selection and recombination
US6309883B1 (en) 1994-02-17 2001-10-30 Maxygen, Inc. Methods and compositions for cellular and metabolic engineering
KR100568008B1 (ko) 1997-01-17 2006-04-07 맥시겐, 인크. 반복적 서열 재조합에 의한 전세포 및 유기체의 개량 방법
US7148054B2 (en) 1997-01-17 2006-12-12 Maxygen, Inc. Evolution of whole cells and organisms by recursive sequence recombination
US6326204B1 (en) 1997-01-17 2001-12-04 Maxygen, Inc. Evolution of whole cells and organisms by recursive sequence recombination
US6153410A (en) * 1997-03-25 2000-11-28 California Institute Of Technology Recombination of polynucleotide sequences using random or defined primers
EP1036198B1 (en) 1997-12-08 2012-09-26 California Institute Of Technology Method for creating polynucleotide and polypeptide sequences
DE69838106T2 (de) * 1997-12-22 2008-04-03 Dsm Ip Assets B.V. Expressionsklonierung in filamentösen pilzen
US7390619B1 (en) 1998-02-11 2008-06-24 Maxygen, Inc. Optimization of immunomodulatory properties of genetic vaccines
BR9910174A (pt) 1998-05-01 2001-03-06 Maxygen Inc Processo para se obter um gene recombinante otimizado de resistência à praga, biblioteca, e, processo para se obter um organismo que seja patogênico a uma praga de vegetal
EP1104459A1 (en) 1998-08-12 2001-06-06 Maxygen, Inc. Dna shuffling of monooxygenase genes for production of industrial chemicals
US6500639B2 (en) 1998-10-07 2002-12-31 Maxygen, Inc. DNA shuffling to produce nucleic acids for mycotoxin detoxification
WO2000028018A1 (en) 1998-11-10 2000-05-18 Maxygen, Inc. Modified adp-glucose pyrophosphorylase for improvement and optimization of plant phenotypes
US6376246B1 (en) 1999-02-05 2002-04-23 Maxygen, Inc. Oligonucleotide mediated nucleic acid recombination
US6436675B1 (en) 1999-09-28 2002-08-20 Maxygen, Inc. Use of codon-varied oligonucleotide synthesis for synthetic shuffling
US6917882B2 (en) 1999-01-19 2005-07-12 Maxygen, Inc. Methods for making character strings, polynucleotides and polypeptides having desired characteristics
JP2002534966A (ja) 1999-01-19 2002-10-22 マキシジェン， インコーポレイテッド オリゴヌクレオチド媒介核酸組換え
US6961664B2 (en) 1999-01-19 2005-11-01 Maxygen Methods of populating data structures for use in evolutionary simulations
US7024312B1 (en) 1999-01-19 2006-04-04 Maxygen, Inc. Methods for making character strings, polynucleotides and polypeptides having desired characteristics
US7384387B1 (en) 1999-02-11 2008-06-10 Maxygen, Inc. High throughput mass spectrometry
AU3719500A (en) 1999-03-05 2000-09-21 Maxygen, Inc. Recombination of insertion modified nucleic acids
US6531316B1 (en) 1999-03-05 2003-03-11 Maxyag, Inc. Encryption of traits using split gene sequences and engineered genetic elements
US7430477B2 (en) 1999-10-12 2008-09-30 Maxygen, Inc. Methods of populating data structures for use in evolutionary simulations
US6686515B1 (en) 1999-11-23 2004-02-03 Maxygen, Inc. Homologous recombination in plants
US7115712B1 (en) 1999-12-02 2006-10-03 Maxygen, Inc. Cytokine polypeptides
CA2396320A1 (en) 2000-01-11 2001-07-19 Maxygen, Inc. Integrated systems and methods for diversity generation and screening
US7094875B2 (en) 2000-06-23 2006-08-22 Maxygen, Inc. Co-stimulatory polypeptides
US7074590B2 (en) 2000-06-23 2006-07-11 Maxygen, Inc. Chimeric promoters
US6858422B2 (en) 2000-07-13 2005-02-22 Codexis, Inc. Lipase genes
JP4647117B2 (ja) * 2001-03-07 2011-03-09 セレスター・レキシコ・サイエンシズ株式会社 突然変異誘発方法
AU2003258658A1 (en) * 2002-08-29 2004-03-19 Andreas Beck Improved dna and proteins
DE102005037351B3 (de) * 2005-08-08 2007-01-11 Geneart Ag Verfahren für die kontinuierliche zielgerichtete Evolution von Proteinen in vitro
EP1926816A1 (en) * 2005-09-21 2008-06-04 Meyer, Andreas In vivo generation of dna, rna, peptide, and protein libraries
KR101098032B1 (ko) * 2008-04-22 2011-12-23 한국생명공학연구원 4차 연속 피씨알, 4차 블록 피씨알, 또는 유전자 합성방법을 이용한 균주 특이적 바코드를 포함하는 유전자 적중 이형접합체 분열효모 균주의 제조방법
CN101532181B (zh) * 2009-03-13 2011-07-20 南京仙奕基因科技有限公司 原位构建基因突变文库的方法及试剂盒
CN105283588A (zh) * 2013-02-26 2016-01-27 艾希奥美公司 用于产生用于定向进化的文库的方法
CN110358786B (zh) * 2018-03-07 2022-02-01 江南大学 一种酶突变体
CN111574633B (zh) * 2020-05-15 2022-04-08 清华大学 一种具有广谱抗病毒功能的蛋白质CbAE的应用
CN114921440B (zh) * 2022-05-16 2023-05-05 北京昕大洋科技发展有限公司 一种木聚糖酶突变体及其应用

Family Cites Families (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
FR2641793B1 (fr) * 1988-12-26 1993-10-01 Setratech Procede de recombinaison in vivo de sequences d'adn presentant des mesappariements de bases

Also Published As

Publication number Publication date
JP2000502568A (ja) 2000-03-07
EP0873398A1 (en) 1998-10-28
CN101070537A (zh) 2007-11-14
ATE293684T1 (de) 2005-05-15
CN1210557A (zh) 1999-03-10
WO1997025410A1 (en) 1997-07-17
EP0873398B1 (en) 2005-04-20
DK0873398T3 (da) 2005-08-15
DE69733059D1 (de) 2005-05-25
DE69733059T2 (de) 2006-03-02

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