CN101070537A - 用于在细胞中体内产生突变体文库方法 - Google Patents

用于在细胞中体内产生突变体文库方法 Download PDF

Info

Publication number
CN101070537A
CN101070537A CNA2006101101626A CN200610110162A CN101070537A CN 101070537 A CN101070537 A CN 101070537A CN A2006101101626 A CNA2006101101626 A CN A2006101101626A CN 200610110162 A CN200610110162 A CN 200610110162A CN 101070537 A CN101070537 A CN 101070537A
Authority
CN
China
Prior art keywords
polypeptide
interest
dna
variant
enzyme
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Pending
Application number
CNA2006101101626A
Other languages
English (en)
Chinese (zh)
Inventor
T·V·伯彻特
S·D·埃尔利克
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Novozymes AS
Original Assignee
Novozymes AS
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Novozymes AS filed Critical Novozymes AS
Publication of CN101070537A publication Critical patent/CN101070537A/zh
Pending legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2521/00—Reaction characterised by the enzymatic activity
    • C12Q2521/10—Nucleotidyl transfering
    • C12Q2521/101—DNA polymerase

Landscapes

  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Biotechnology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • General Engineering & Computer Science (AREA)
  • Wood Science & Technology (AREA)
  • Biomedical Technology (AREA)
  • Microbiology (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Plant Pathology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
CNA2006101101626A 1996-01-10 1997-01-10 用于在细胞中体内产生突变体文库方法 Pending CN101070537A (zh)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
DK0018/96 1996-01-10
DK1896 1996-01-10

Related Parent Applications (1)

Application Number Title Priority Date Filing Date
CN97191974A Division CN1210557A (zh) 1996-01-10 1997-01-10 用于在细胞中体内产生突变体文库方法

Publications (1)

Publication Number Publication Date
CN101070537A true CN101070537A (zh) 2007-11-14

Family

ID=8088824

Family Applications (2)

Application Number Title Priority Date Filing Date
CNA2006101101626A Pending CN101070537A (zh) 1996-01-10 1997-01-10 用于在细胞中体内产生突变体文库方法
CN97191974A Pending CN1210557A (zh) 1996-01-10 1997-01-10 用于在细胞中体内产生突变体文库方法

Family Applications After (1)

Application Number Title Priority Date Filing Date
CN97191974A Pending CN1210557A (zh) 1996-01-10 1997-01-10 用于在细胞中体内产生突变体文库方法

Country Status (8)

Country Link
EP (1) EP0873398B1 (enExample)
JP (1) JP2000502568A (enExample)
CN (2) CN101070537A (enExample)
AT (1) ATE293684T1 (enExample)
AU (1) AU1367897A (enExample)
DE (1) DE69733059T2 (enExample)
DK (1) DK0873398T3 (enExample)
WO (1) WO1997025410A1 (enExample)

Cited By (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN102016022B (zh) * 2008-04-22 2014-05-14 韩国生命工学研究院 通过4轮连续pcr或阻断pcr或其全基因合成的方法进行的含有基因特异的条形码的粟酒裂殖酵母杂合缺失突变体的全基因组构建
CN111574633A (zh) * 2020-05-15 2020-08-25 清华大学 一种具有广谱抗病毒功能的蛋白质CbAE的应用
CN114921440B (zh) * 2022-05-16 2023-05-05 北京昕大洋科技发展有限公司 一种木聚糖酶突变体及其应用

Families Citing this family (42)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6995017B1 (en) 1994-02-17 2006-02-07 Maxygen, Inc. Methods for generating polynucleotides having desired characteristics by iterative selection and recombination
US5837458A (en) 1994-02-17 1998-11-17 Maxygen, Inc. Methods and compositions for cellular and metabolic engineering
US6309883B1 (en) 1994-02-17 2001-10-30 Maxygen, Inc. Methods and compositions for cellular and metabolic engineering
US6165793A (en) * 1996-03-25 2000-12-26 Maxygen, Inc. Methods for generating polynucleotides having desired characteristics by iterative selection and recombination
US6335160B1 (en) 1995-02-17 2002-01-01 Maxygen, Inc. Methods and compositions for polypeptide engineering
US20060257890A1 (en) 1996-05-20 2006-11-16 Maxygen, Inc. Methods and compositions for cellular and metabolic engineering
US5605793A (en) * 1994-02-17 1997-02-25 Affymax Technologies N.V. Methods for in vitro recombination
US6117679A (en) 1994-02-17 2000-09-12 Maxygen, Inc. Methods for generating polynucleotides having desired characteristics by iterative selection and recombination
AU743305C (en) 1997-01-17 2006-03-30 Maxygen, Inc. Evolution of whole cells and organisms by recursive sequence recombination
US7148054B2 (en) 1997-01-17 2006-12-12 Maxygen, Inc. Evolution of whole cells and organisms by recursive sequence recombination
US6326204B1 (en) 1997-01-17 2001-12-04 Maxygen, Inc. Evolution of whole cells and organisms by recursive sequence recombination
US6153410A (en) * 1997-03-25 2000-11-28 California Institute Of Technology Recombination of polynucleotide sequences using random or defined primers
IL136574A0 (en) 1997-12-08 2001-06-14 California Inst Of Techn A method for forming a polynucleotide of desired properties
JP4410413B2 (ja) * 1997-12-22 2010-02-03 コニンクリーケ デーエスエム ナムローゼ フェンノートシャップ 糸状菌での発現クローニング
US7390619B1 (en) 1998-02-11 2008-06-24 Maxygen, Inc. Optimization of immunomodulatory properties of genetic vaccines
EP1073670A1 (en) 1998-05-01 2001-02-07 Maxygen, Inc. Optimization of pest resistance genes using dna shuffling
US6605430B1 (en) 1998-08-12 2003-08-12 Maxygen, Inc. DNA shuffling of monooxygenase genes for production of industrial chemicals
JP2002526107A (ja) 1998-10-07 2002-08-20 マキシジェン， インコーポレイテッド マイコトキシンの解毒のための核酸を生成するためのｄｎａシャッフリング
EP1129184A1 (en) 1998-11-10 2001-09-05 Maxygen, Inc. Modified adp-glucose pyrophosphorylase for improvement and optimation of plant phenotypes
US6376246B1 (en) 1999-02-05 2002-04-23 Maxygen, Inc. Oligonucleotide mediated nucleic acid recombination
US6917882B2 (en) 1999-01-19 2005-07-12 Maxygen, Inc. Methods for making character strings, polynucleotides and polypeptides having desired characteristics
US6436675B1 (en) 1999-09-28 2002-08-20 Maxygen, Inc. Use of codon-varied oligonucleotide synthesis for synthetic shuffling
IL137868A0 (en) 1999-01-19 2001-10-31 Maxygen Inc Oligonucleotide mediated nucleic acid recombination
US7024312B1 (en) 1999-01-19 2006-04-04 Maxygen, Inc. Methods for making character strings, polynucleotides and polypeptides having desired characteristics
US6961664B2 (en) 1999-01-19 2005-11-01 Maxygen Methods of populating data structures for use in evolutionary simulations
IL144657A0 (en) 1999-02-11 2002-06-30 Maxygen Inc High throughput mass spectrometry
AU3719500A (en) 1999-03-05 2000-09-21 Maxygen, Inc. Recombination of insertion modified nucleic acids
US6531316B1 (en) 1999-03-05 2003-03-11 Maxyag, Inc. Encryption of traits using split gene sequences and engineered genetic elements
US7430477B2 (en) 1999-10-12 2008-09-30 Maxygen, Inc. Methods of populating data structures for use in evolutionary simulations
US6686515B1 (en) 1999-11-23 2004-02-03 Maxygen, Inc. Homologous recombination in plants
US7115712B1 (en) 1999-12-02 2006-10-03 Maxygen, Inc. Cytokine polypeptides
SG121902A1 (en) 2000-01-11 2006-05-26 Maxygen Inc Integrated systems for diversity generation and screening
AU2001268716A1 (en) 2000-06-23 2002-01-08 Maxygen, Inc. Novel chimeric promoters
EP1360290A2 (en) 2000-06-23 2003-11-12 Maxygen, Inc. Co-stimulatory molecules
US6858422B2 (en) 2000-07-13 2005-02-22 Codexis, Inc. Lipase genes
JP4647117B2 (ja) * 2001-03-07 2011-03-09 セレスター・レキシコ・サイエンシズ株式会社 突然変異誘発方法
WO2004020627A2 (en) * 2002-08-29 2004-03-11 Andreas Beck Improved dna and proteins
DE102005037351B3 (de) * 2005-08-08 2007-01-11 Geneart Ag Verfahren für die kontinuierliche zielgerichtete Evolution von Proteinen in vitro
WO2007039461A1 (en) * 2005-09-21 2007-04-12 Andreas Meyer In vivo generation of dna, rna, peptide, and protein libraries
CN101532181B (zh) * 2009-03-13 2011-07-20 南京仙奕基因科技有限公司 原位构建基因突变文库的方法及试剂盒
SG11201506696TA (en) 2013-02-26 2015-09-29 Axiomx Inc Methods for the production of libraries for directed evolution
CN110373404B (zh) * 2018-03-07 2021-11-16 江南大学 一种酶突变体

Family Cites Families (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
FR2641793B1 (fr) * 1988-12-26 1993-10-01 Setratech Procede de recombinaison in vivo de sequences d'adn presentant des mesappariements de bases

Cited By (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN102016022B (zh) * 2008-04-22 2014-05-14 韩国生命工学研究院 通过4轮连续pcr或阻断pcr或其全基因合成的方法进行的含有基因特异的条形码的粟酒裂殖酵母杂合缺失突变体的全基因组构建
CN111574633A (zh) * 2020-05-15 2020-08-25 清华大学 一种具有广谱抗病毒功能的蛋白质CbAE的应用
CN111574633B (zh) * 2020-05-15 2022-04-08 清华大学 一种具有广谱抗病毒功能的蛋白质CbAE的应用
CN114921440B (zh) * 2022-05-16 2023-05-05 北京昕大洋科技发展有限公司 一种木聚糖酶突变体及其应用

Also Published As

Publication number Publication date
EP0873398B1 (en) 2005-04-20
JP2000502568A (ja) 2000-03-07
WO1997025410A1 (en) 1997-07-17
DE69733059D1 (de) 2005-05-25
AU1367897A (en) 1997-08-01
CN1210557A (zh) 1999-03-10
DK0873398T3 (da) 2005-08-15
EP0873398A1 (en) 1998-10-28
ATE293684T1 (de) 2005-05-15
DE69733059T2 (de) 2006-03-02

Similar Documents

Publication Publication Date Title
CN101070537A (zh) 用于在细胞中体内产生突变体文库方法
US11781147B2 (en) Promoter sequences and methods thereof for enhanced protein production in Bacillus cells
US12281316B2 (en) Methods and compositions for efficient genetic modifications of Bacillus licheniformis strains
JP7787077B2 (ja) バチルス・リケニフォルミス（ｂａｃｉｌｌｕｓ ｌｉｃｈｅｎｉｆｏｒｍｉｓ）における強化したタンパク質産生のための組成物及び方法
JP2020534821A (ja) バチルス属（Ｂａｃｉｌｌｕｓ）におけるタンパク質産生の増加のための改変５’−非翻訳領域（ＵＴＲ）配列
JP2001514529A (ja) プロテアーゼ活性を有するポリペプチドをコードする核酸
US6500644B1 (en) Method for in vivo production of a mutant library in cells
ES2703753T3 (es) Procedimiento de expresión
US11414643B2 (en) Mutant and genetically modified Bacillus cells and methods thereof for increased protein production
JP2022526414A (ja) 二重環状組換えＤＮＡコンストラクトを使用するバチルス（Ｂａｃｉｌｌｕｓ）のゲノムへのポリヌクレオチド組込みのための方法及びその組成物
CN107278230B (zh) 增强的蛋白质表达
CN107250371B (zh) 增强的蛋白质表达
CN103154263A (zh) 诱导的孢子形成筛选方法
US8846403B2 (en) Method of screening for protein secretion recombinant host cells
US20220389372A1 (en) Compositions and methods for enhanced protein production in bacillus cells
US20220282234A1 (en) Compositions and methods for increased protein production in bacillus lichenformis
JP4312832B2 (ja) インビボ組換え
Lopez-Camacho Modificaci\'on funcional de beta-glicosidasas bacterianas.

Legal Events

Date Code Title Description
C06 Publication
PB01 Publication
C10 Entry into substantive examination
SE01 Entry into force of request for substantive examination
C02 Deemed withdrawal of patent application after publication (patent law 2001)
WD01 Invention patent application deemed withdrawn after publication

Open date: 20071114