ATE293684T1 - Methode zur invivo produktion einer mutanten- bibliothek in zellen - Google Patents

Methode zur invivo produktion einer mutanten- bibliothek in zellen

Info

Publication number
ATE293684T1
ATE293684T1 AT97900206T AT97900206T ATE293684T1 AT E293684 T1 ATE293684 T1 AT E293684T1 AT 97900206 T AT97900206 T AT 97900206T AT 97900206 T AT97900206 T AT 97900206T AT E293684 T1 ATE293684 T1 AT E293684T1
Authority
AT
Austria
Prior art keywords
cells
replication
production
invivo
genetic
Prior art date
Application number
AT97900206T
Other languages
German (de)
English (en)
Inventor
Torben Vedel Borchert
Stanislas Dusko Ehrlich
Agronomique
Original Assignee
Novozymes As
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Novozymes As filed Critical Novozymes As
Application granted granted Critical
Publication of ATE293684T1 publication Critical patent/ATE293684T1/de

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2521/00—Reaction characterised by the enzymatic activity
    • C12Q2521/10—Nucleotidyl transfering
    • C12Q2521/101—DNA polymerase

Landscapes

  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Biotechnology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • General Engineering & Computer Science (AREA)
  • Wood Science & Technology (AREA)
  • Biomedical Technology (AREA)
  • Microbiology (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Plant Pathology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
AT97900206T 1996-01-10 1997-01-10 Methode zur invivo produktion einer mutanten- bibliothek in zellen ATE293684T1 (de)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
DK1896 1996-01-10
PCT/DK1997/000014 WO1997025410A1 (en) 1996-01-10 1997-01-10 A method for in vivo production of a mutant library in cells

Publications (1)

Publication Number Publication Date
ATE293684T1 true ATE293684T1 (de) 2005-05-15

Family

ID=8088824

Family Applications (1)

Application Number Title Priority Date Filing Date
AT97900206T ATE293684T1 (de) 1996-01-10 1997-01-10 Methode zur invivo produktion einer mutanten- bibliothek in zellen

Country Status (8)

Country Link
EP (1) EP0873398B1 (enExample)
JP (1) JP2000502568A (enExample)
CN (2) CN1210557A (enExample)
AT (1) ATE293684T1 (enExample)
AU (1) AU1367897A (enExample)
DE (1) DE69733059T2 (enExample)
DK (1) DK0873398T3 (enExample)
WO (1) WO1997025410A1 (enExample)

Families Citing this family (45)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5837458A (en) 1994-02-17 1998-11-17 Maxygen, Inc. Methods and compositions for cellular and metabolic engineering
US5605793A (en) * 1994-02-17 1997-02-25 Affymax Technologies N.V. Methods for in vitro recombination
US20060257890A1 (en) 1996-05-20 2006-11-16 Maxygen, Inc. Methods and compositions for cellular and metabolic engineering
US6309883B1 (en) 1994-02-17 2001-10-30 Maxygen, Inc. Methods and compositions for cellular and metabolic engineering
US6335160B1 (en) 1995-02-17 2002-01-01 Maxygen, Inc. Methods and compositions for polypeptide engineering
US6995017B1 (en) 1994-02-17 2006-02-07 Maxygen, Inc. Methods for generating polynucleotides having desired characteristics by iterative selection and recombination
US6165793A (en) * 1996-03-25 2000-12-26 Maxygen, Inc. Methods for generating polynucleotides having desired characteristics by iterative selection and recombination
US6117679A (en) 1994-02-17 2000-09-12 Maxygen, Inc. Methods for generating polynucleotides having desired characteristics by iterative selection and recombination
US6326204B1 (en) 1997-01-17 2001-12-04 Maxygen, Inc. Evolution of whole cells and organisms by recursive sequence recombination
ES2270505T3 (es) 1997-01-17 2007-04-01 Maxygen, Inc. Evolucion de celulas completas procariotidas por recombinacion de secuencias recursivas.
US7148054B2 (en) 1997-01-17 2006-12-12 Maxygen, Inc. Evolution of whole cells and organisms by recursive sequence recombination
US6153410A (en) * 1997-03-25 2000-11-28 California Institute Of Technology Recombination of polynucleotide sequences using random or defined primers
IL136574A0 (en) 1997-12-08 2001-06-14 California Inst Of Techn A method for forming a polynucleotide of desired properties
JP4410413B2 (ja) * 1997-12-22 2010-02-03 コニンクリーケ デーエスエム ナムローゼ フェンノートシャップ 糸状菌での発現クローニング
US7390619B1 (en) 1998-02-11 2008-06-24 Maxygen, Inc. Optimization of immunomodulatory properties of genetic vaccines
JP2002513550A (ja) 1998-05-01 2002-05-14 マキシジェン， インコーポレイテッド Ｄｎａシャッフリングを使用する病害虫抵抗性遺伝子の至適化
CA2332615A1 (en) 1998-08-12 2000-02-24 Maxygen Inc. Dna shuffling of monooxygenase genes for production of industrial chemicals
CA2345203A1 (en) 1998-10-07 2000-04-13 Maxygen Inc. Dna shuffling to produce nucleic acids for mycotoxin detoxification
WO2000028018A1 (en) 1998-11-10 2000-05-18 Maxygen, Inc. Modified adp-glucose pyrophosphorylase for improvement and optimization of plant phenotypes
US6436675B1 (en) 1999-09-28 2002-08-20 Maxygen, Inc. Use of codon-varied oligonucleotide synthesis for synthetic shuffling
US6917882B2 (en) 1999-01-19 2005-07-12 Maxygen, Inc. Methods for making character strings, polynucleotides and polypeptides having desired characteristics
US6376246B1 (en) 1999-02-05 2002-04-23 Maxygen, Inc. Oligonucleotide mediated nucleic acid recombination
US7024312B1 (en) 1999-01-19 2006-04-04 Maxygen, Inc. Methods for making character strings, polynucleotides and polypeptides having desired characteristics
US6961664B2 (en) 1999-01-19 2005-11-01 Maxygen Methods of populating data structures for use in evolutionary simulations
ATE465459T1 (de) 1999-01-19 2010-05-15 Maxygen Inc Durch oligonukleotide-vermittelte nukleinsäuren- rekombination
IL144657A0 (en) 1999-02-11 2002-06-30 Maxygen Inc High throughput mass spectrometry
US6531316B1 (en) 1999-03-05 2003-03-11 Maxyag, Inc. Encryption of traits using split gene sequences and engineered genetic elements
AU3391900A (en) 1999-03-05 2000-09-21 Maxygen, Inc. Encryption of traits using split gene sequences
US7430477B2 (en) 1999-10-12 2008-09-30 Maxygen, Inc. Methods of populating data structures for use in evolutionary simulations
US6686515B1 (en) 1999-11-23 2004-02-03 Maxygen, Inc. Homologous recombination in plants
US7115712B1 (en) 1999-12-02 2006-10-03 Maxygen, Inc. Cytokine polypeptides
EP1276900A2 (en) 2000-01-11 2003-01-22 Maxygen, Inc. Integrated systems and methods for diversity generation and screening
US7074590B2 (en) 2000-06-23 2006-07-11 Maxygen, Inc. Chimeric promoters
CA2411828A1 (en) 2000-06-23 2002-01-03 Maxygen, Inc. Novel co-stimulatory molecules
US6858422B2 (en) 2000-07-13 2005-02-22 Codexis, Inc. Lipase genes
JP4647117B2 (ja) * 2001-03-07 2011-03-09 セレスター・レキシコ・サイエンシズ株式会社 突然変異誘発方法
AU2003258658A1 (en) * 2002-08-29 2004-03-19 Andreas Beck Improved dna and proteins
DE102005037351B3 (de) * 2005-08-08 2007-01-11 Geneart Ag Verfahren für die kontinuierliche zielgerichtete Evolution von Proteinen in vitro
WO2007039461A1 (en) * 2005-09-21 2007-04-12 Andreas Meyer In vivo generation of dna, rna, peptide, and protein libraries
KR101098032B1 (ko) * 2008-04-22 2011-12-23 한국생명공학연구원 4차 연속 피씨알, 4차 블록 피씨알, 또는 유전자 합성방법을 이용한 균주 특이적 바코드를 포함하는 유전자 적중 이형접합체 분열효모 균주의 제조방법
CN101532181B (zh) * 2009-03-13 2011-07-20 南京仙奕基因科技有限公司 原位构建基因突变文库的方法及试剂盒
US9422549B2 (en) 2013-02-26 2016-08-23 Axiomx, Inc. Methods for the production of libraries for directed evolution
CN108342407A (zh) * 2018-03-07 2018-07-31 江南大学 一种得到高表达、高活力及高稳定性的酶突变体的方法
CN111574633B (zh) * 2020-05-15 2022-04-08 清华大学 一种具有广谱抗病毒功能的蛋白质CbAE的应用
CN114921440B (zh) * 2022-05-16 2023-05-05 北京昕大洋科技发展有限公司 一种木聚糖酶突变体及其应用

Family Cites Families (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
FR2641793B1 (fr) * 1988-12-26 1993-10-01 Setratech Procede de recombinaison in vivo de sequences d'adn presentant des mesappariements de bases

Also Published As

Publication number Publication date
EP0873398B1 (en) 2005-04-20
DE69733059D1 (de) 2005-05-25
DK0873398T3 (da) 2005-08-15
AU1367897A (en) 1997-08-01
JP2000502568A (ja) 2000-03-07
WO1997025410A1 (en) 1997-07-17
CN1210557A (zh) 1999-03-10
DE69733059T2 (de) 2006-03-02
CN101070537A (zh) 2007-11-14
EP0873398A1 (en) 1998-10-28

Similar Documents

Publication Publication Date Title
DE69733059D1 (de) Methode zur invivo produktion einer mutanten-bibliothek in zellen
Pavlı́ček et al. Similar integration but different stability of Alus and LINEs in the human genome
DE69739192D1 (de) Indexierte zell-bibliothek mit genomischen veränderungen und verfahren zur deren herstellung und verwendung
National Research Council et al. Mapping and sequencing the human genome
Wolstenholme et al. Sequence evolution of Drosophila mitochondrial DNA
Filipski Correlation between molecular clock ticking, codon usage, fidelity of DNA repair, chromosome banding and chromatin compactness in germline cells
ATE414782T1 (de) Verfahren zur herstellung von polypeptiden in mutierten aspergillus zellen
Ferguson et al. Characterization of a hotspot for mimicry: assembly of a butterfly wing transcriptome to genomic sequence at the HmYb/Sb locus
Giguere et al. Telomere-to-telomere genome assembly of Phaeodactylum tricornutum
DE69918820D1 (de) Dna hochdurchsatzvektor für sequenzierung
ATE374827T1 (de) Überexpression von gewünschten proteinen in eukaryotischen zellen mittels überexpression von cyclin d1
Kuehn et al. Nucleotide sequence of the genetically labile repeated elements 5′ to the origin of mouse rRNA transcription
Flintoff et al. Moderate-level gene amplification in methotrexate-resistant Chinese hamster ovary cells is accompanied by chromosomal translocations at or near the site of the amplified DHFR gene
DE69723855D1 (de) Verfahren zur herstellung von polypeptiden in surfaktinmutanten von bacillus zellen
Cappelletti et al. Robertsonian fusion and centromere repositioning contributed to the formation of satellite-free centromeres during the evolution of zebras
Lorenzi et al. Subtelomeres are fast-evolving regions of the Streptomyces linear chromosome
Hou et al. Novel transfer RNAs that are active in Escherichia coli
Loveless et al. DNA writing at a single genomic site enables lineage tracing and analog recording in mammalian cells
Wild et al. Targeting and retrofitting pre-existing libraries of transposon insertions with FRT and oriV elements for in-vivo generation of large quantities of any genomic fragment
Landy et al. Site-specific Recombination in Bacteriophage λ: Structural Features of Recombining Sites
DE60331644D1 (de) Verfahren zur herstellung von varianten einer dna-sequenz in filamentösen pilzen
Li et al. piggyBac transposon-based insertional mutagenesis for the fission yeast Schizosaccharomyces pombe
Robbins Representing genomic maps in a relational database
Pladevall et al. The genome sequence of the Violet Copper, Lycaena helle (Denis & Schiffermüller, 1775)
Witkowski Milestones in the Development of DNA Technology

Legal Events

Date Code Title Description
RER Ceased as to paragraph 5 lit. 3 law introducing patent treaties