SG10201706943PA - Methods for the production of libraries for directed evolution - Google Patents

Methods for the production of libraries for directed evolution

Info

Publication number
SG10201706943PA
SG10201706943PA SG10201706943PA SG10201706943PA SG10201706943PA SG 10201706943P A SG10201706943P A SG 10201706943PA SG 10201706943P A SG10201706943P A SG 10201706943PA SG 10201706943P A SG10201706943P A SG 10201706943PA SG 10201706943P A SG10201706943P A SG 10201706943PA
Authority
SG
Singapore
Prior art keywords
libraries
production
methods
directed evolution
evolution
Prior art date
Application number
SG10201706943PA
Inventor
Michael Weiner
Margaret Kiss
Original Assignee
Axiomx Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Axiomx Inc filed Critical Axiomx Inc
Publication of SG10201706943PA publication Critical patent/SG10201706943PA/en

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1034—Isolating an individual clone by screening libraries
    • C12N15/1058—Directional evolution of libraries, e.g. evolution of libraries is achieved by mutagenesis and screening or selection of mixed population of organisms
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/102—Mutagenizing nucleic acids
    • C12N15/1024—In vivo mutagenesis using high mutation rate "mutator" host strains by inserting genetic material, e.g. encoding an error prone polymerase, disrupting a gene for mismatch repair
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1034—Isolating an individual clone by screening libraries
    • C12N15/1037—Screening libraries presented on the surface of microorganisms, e.g. phage display, E. coli display
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1034—Isolating an individual clone by screening libraries
    • C12N15/1068—Template (nucleic acid) mediated chemical library synthesis, e.g. chemical and enzymatical DNA-templated organic molecule synthesis, libraries prepared by non ribosomal polypeptide synthesis [NRPS], DNA/RNA-polymerase mediated polypeptide synthesis
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1034—Isolating an individual clone by screening libraries
    • C12N15/1093—General methods of preparing gene libraries, not provided for in other subgroups
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/87—Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation
    • C12N15/90—Stable introduction of foreign DNA into chromosome
    • C12N15/902—Stable introduction of foreign DNA into chromosome using homologous recombination
    • C12N15/907—Stable introduction of foreign DNA into chromosome using homologous recombination in mammalian cells
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2317/00—Immunoglobulins specific features
    • C07K2317/10—Immunoglobulins specific features characterized by their source of isolation or production
    • C07K2317/14—Specific host cells or culture conditions, e.g. components, pH or temperature
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2317/00—Immunoglobulins specific features
    • C07K2317/50—Immunoglobulins specific features characterized by immunoglobulin fragments
    • C07K2317/51—Complete heavy chain or Fd fragment, i.e. VH + CH1
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2317/00—Immunoglobulins specific features
    • C07K2317/50—Immunoglobulins specific features characterized by immunoglobulin fragments
    • C07K2317/515—Complete light chain, i.e. VL + CL
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2317/00—Immunoglobulins specific features
    • C07K2317/60—Immunoglobulins specific features characterized by non-natural combinations of immunoglobulin fragments
    • C07K2317/62—Immunoglobulins specific features characterized by non-natural combinations of immunoglobulin fragments comprising only variable region components
    • C07K2317/622—Single chain antibody (scFv)
    • C—CHEMISTRY; METALLURGY
    • C40—COMBINATORIAL TECHNOLOGY
    • C40B—COMBINATORIAL CHEMISTRY; LIBRARIES, e.g. CHEMICAL LIBRARIES
    • C40B30/00—Methods of screening libraries
    • C40B30/04—Methods of screening libraries by measuring the ability to specifically bind a target molecule, e.g. antibody-antigen binding, receptor-ligand binding
    • C—CHEMISTRY; METALLURGY
    • C40—COMBINATORIAL TECHNOLOGY
    • C40B—COMBINATORIAL CHEMISTRY; LIBRARIES, e.g. CHEMICAL LIBRARIES
    • C40B50/00—Methods of creating libraries, e.g. combinatorial synthesis
    • C40B50/06—Biochemical methods, e.g. using enzymes or whole viable microorganisms

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Biomedical Technology (AREA)
  • Zoology (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Biophysics (AREA)
  • Microbiology (AREA)
  • Physics & Mathematics (AREA)
  • Plant Pathology (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Bioinformatics & Computational Biology (AREA)
  • Ecology (AREA)
  • Medicinal Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Immunology (AREA)
  • Cell Biology (AREA)
  • Mycology (AREA)
  • Virology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
SG10201706943PA 2013-02-26 2014-02-26 Methods for the production of libraries for directed evolution SG10201706943PA (en)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
US201361769517P 2013-02-26 2013-02-26

Publications (1)

Publication Number Publication Date
SG10201706943PA true SG10201706943PA (en) 2017-10-30

Family

ID=51428767

Family Applications (2)

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SG10201706943PA SG10201706943PA (en) 2013-02-26 2014-02-26 Methods for the production of libraries for directed evolution
SG11201506696TA SG11201506696TA (en) 2013-02-26 2014-02-26 Methods for the production of libraries for directed evolution

Family Applications After (1)

Application Number Title Priority Date Filing Date
SG11201506696TA SG11201506696TA (en) 2013-02-26 2014-02-26 Methods for the production of libraries for directed evolution

Country Status (10)

Country Link
US (4) US9422549B2 (en)
EP (1) EP2961866B1 (en)
JP (1) JP6552969B2 (en)
KR (1) KR20150140663A (en)
CN (1) CN105283588A (en)
AU (2) AU2014223602C1 (en)
CA (1) CA2902189C (en)
IL (1) IL240742A0 (en)
SG (2) SG10201706943PA (en)
WO (1) WO2014134166A1 (en)

Families Citing this family (11)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2014100419A1 (en) 2012-12-20 2014-06-26 Axiomx, Inc. Compositions and methods for the identification and isolation of cell-membrane protein specific binding moieties
SG10201706943PA (en) 2013-02-26 2017-10-30 Axiomx Inc Methods for the production of libraries for directed evolution
US11473080B2 (en) 2014-04-30 2022-10-18 The Board Of Trustees Of The University Of Illinois Method for generating high affinity, bivalent binding agents for sandwich assays
US20150315566A1 (en) * 2014-04-30 2015-11-05 The Board Of Trustees Of The University Of Illinois Method for generating high affinity, bivalent binding agents
CA2989143A1 (en) 2015-06-12 2016-12-15 Axiomx, Inc. Methods and compositions for producing a chimeric polypeptide
CN107794572B (en) * 2016-08-31 2022-04-05 安诺优达基因科技(北京)有限公司 Method for constructing large fragment library and application thereof
CN108203847B (en) * 2016-12-16 2022-01-04 深圳华大智造科技股份有限公司 Library, reagent and application for second-generation sequencing quality evaluation
US20210032368A1 (en) 2017-08-04 2021-02-04 Abcam Plc Methods and compositions for the development of antibodies specific to epitope post-translational modification status
US10350307B2 (en) * 2017-09-18 2019-07-16 General Electric Company In vivo RNA or protein expression using double-stranded concatemeric DNA including phosphorothioated nucleotides
CN112063688A (en) * 2020-07-28 2020-12-11 翌圣生物科技（上海）有限公司 Method for preparing annular ssDNA and kit thereof
WO2023230553A2 (en) * 2022-05-26 2023-11-30 Illumina, Inc. Preparation of long read nucleic acid libraries

Family Cites Families (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
AU1367897A (en) * 1996-01-10 1997-08-01 Novo Nordisk A/S A method for in vivo production of a mutant library in cells
CA2448628A1 (en) 2001-05-30 2002-12-05 Stratagene Compositions and methods for random nucleic acid mutagenesis
FI20096371A0 (en) * 2009-12-21 2009-12-21 Turun Yliopisto Method of Mutagenization
WO2014100419A1 (en) 2012-12-20 2014-06-26 Axiomx, Inc. Compositions and methods for the identification and isolation of cell-membrane protein specific binding moieties
SG10201706943PA (en) 2013-02-26 2017-10-30 Axiomx Inc Methods for the production of libraries for directed evolution
EP3077508B1 (en) 2013-12-04 2018-11-14 AxioMx, Inc. Methods of utilizing recombination for the identification of binding moieties

Also Published As

Publication number Publication date
EP2961866B1 (en) 2021-04-21
CA2902189C (en) 2023-05-23
AU2018256545A1 (en) 2018-11-22
US11066662B2 (en) 2021-07-20
US9617537B2 (en) 2017-04-11
EP2961866A1 (en) 2016-01-06
JP6552969B2 (en) 2019-07-31
US20160032280A1 (en) 2016-02-04
JP2016507252A (en) 2016-03-10
US20160304859A1 (en) 2016-10-20
CN105283588A (en) 2016-01-27
AU2014223602C1 (en) 2018-11-08
AU2018256545B2 (en) 2021-05-27
US20190345486A1 (en) 2019-11-14
KR20150140663A (en) 2015-12-16
US9422549B2 (en) 2016-08-23
CA2902189A1 (en) 2014-09-04
EP2961866A4 (en) 2017-03-01
US10301617B2 (en) 2019-05-28
AU2014223602A1 (en) 2015-09-10
WO2014134166A9 (en) 2015-09-17
US20170204404A1 (en) 2017-07-20
SG11201506696TA (en) 2015-09-29
IL240742A0 (en) 2015-10-29
WO2014134166A1 (en) 2014-09-04
AU2014223602B2 (en) 2018-08-02

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