DK0873398T3 - Fremgangsmåde til in vivo produktion af et mutantbibliotek i celler - Google Patents

Fremgangsmåde til in vivo produktion af et mutantbibliotek i celler

Info

Publication number
DK0873398T3
DK0873398T3 DK97900206T DK97900206T DK0873398T3 DK 0873398 T3 DK0873398 T3 DK 0873398T3 DK 97900206 T DK97900206 T DK 97900206T DK 97900206 T DK97900206 T DK 97900206T DK 0873398 T3 DK0873398 T3 DK 0873398T3
Authority
DK
Denmark
Prior art keywords
cells
replication
vivo production
genetic
polypeptide
Prior art date
Application number
DK97900206T
Other languages
Danish (da)
English (en)
Inventor
Torben Vedel Borchert
Stanislas Dusko Ehrlich
Original Assignee
Novozymes As
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Novozymes As filed Critical Novozymes As
Application granted granted Critical
Publication of DK0873398T3 publication Critical patent/DK0873398T3/da

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2521/00—Reaction characterised by the enzymatic activity
    • C12Q2521/10—Nucleotidyl transfering
    • C12Q2521/101—DNA polymerase

Landscapes

  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Biotechnology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • General Engineering & Computer Science (AREA)
  • Wood Science & Technology (AREA)
  • Biomedical Technology (AREA)
  • Microbiology (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Plant Pathology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
DK97900206T 1996-01-10 1997-01-10 Fremgangsmåde til in vivo produktion af et mutantbibliotek i celler DK0873398T3 (da)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
DK1896 1996-01-10
PCT/DK1997/000014 WO1997025410A1 (en) 1996-01-10 1997-01-10 A method for in vivo production of a mutant library in cells

Publications (1)

Publication Number Publication Date
DK0873398T3 true DK0873398T3 (da) 2005-08-15

Family

ID=8088824

Family Applications (1)

Application Number Title Priority Date Filing Date
DK97900206T DK0873398T3 (da) 1996-01-10 1997-01-10 Fremgangsmåde til in vivo produktion af et mutantbibliotek i celler

Country Status (8)

Country Link
EP (1) EP0873398B1 (enExample)
JP (1) JP2000502568A (enExample)
CN (2) CN1210557A (enExample)
AT (1) ATE293684T1 (enExample)
AU (1) AU1367897A (enExample)
DE (1) DE69733059T2 (enExample)
DK (1) DK0873398T3 (enExample)
WO (1) WO1997025410A1 (enExample)

Families Citing this family (45)

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Publication number Priority date Publication date Assignee Title
US5837458A (en) 1994-02-17 1998-11-17 Maxygen, Inc. Methods and compositions for cellular and metabolic engineering
US5605793A (en) 1994-02-17 1997-02-25 Affymax Technologies N.V. Methods for in vitro recombination
US6995017B1 (en) 1994-02-17 2006-02-07 Maxygen, Inc. Methods for generating polynucleotides having desired characteristics by iterative selection and recombination
US20060257890A1 (en) 1996-05-20 2006-11-16 Maxygen, Inc. Methods and compositions for cellular and metabolic engineering
US6309883B1 (en) 1994-02-17 2001-10-30 Maxygen, Inc. Methods and compositions for cellular and metabolic engineering
US6165793A (en) * 1996-03-25 2000-12-26 Maxygen, Inc. Methods for generating polynucleotides having desired characteristics by iterative selection and recombination
US6335160B1 (en) 1995-02-17 2002-01-01 Maxygen, Inc. Methods and compositions for polypeptide engineering
US6117679A (en) * 1994-02-17 2000-09-12 Maxygen, Inc. Methods for generating polynucleotides having desired characteristics by iterative selection and recombination
US7148054B2 (en) 1997-01-17 2006-12-12 Maxygen, Inc. Evolution of whole cells and organisms by recursive sequence recombination
US6326204B1 (en) 1997-01-17 2001-12-04 Maxygen, Inc. Evolution of whole cells and organisms by recursive sequence recombination
DE69835360T2 (de) 1997-01-17 2007-08-16 Maxygen, Inc., Redwood City EVOLUTION Prokaryotischer GANZER ZELLEN DURCH REKURSIVE SEQUENZREKOMBINATION
US6153410A (en) * 1997-03-25 2000-11-28 California Institute Of Technology Recombination of polynucleotide sequences using random or defined primers
JP3712255B2 (ja) 1997-12-08 2005-11-02 カリフォルニア・インスティチュート・オブ・テクノロジー ポリヌクレオチドおよびポリペプチド配列を生成するための方法
ATE367439T1 (de) * 1997-12-22 2007-08-15 Dsm Ip Assets Bv Expressionsklonierung in filamentösen pilzen
US7390619B1 (en) 1998-02-11 2008-06-24 Maxygen, Inc. Optimization of immunomodulatory properties of genetic vaccines
WO1999057128A1 (en) 1998-05-01 1999-11-11 Maxygen, Inc. Optimization of pest resistance genes using dna shuffling
EP1104459A1 (en) 1998-08-12 2001-06-06 Maxygen, Inc. Dna shuffling of monooxygenase genes for production of industrial chemicals
WO2000020573A2 (en) 1998-10-07 2000-04-13 Maxygen, Inc. Dna shuffling to produce nucleic acids for mycotoxin detoxification
WO2000028018A1 (en) 1998-11-10 2000-05-18 Maxygen, Inc. Modified adp-glucose pyrophosphorylase for improvement and optimization of plant phenotypes
US6376246B1 (en) 1999-02-05 2002-04-23 Maxygen, Inc. Oligonucleotide mediated nucleic acid recombination
US6917882B2 (en) 1999-01-19 2005-07-12 Maxygen, Inc. Methods for making character strings, polynucleotides and polypeptides having desired characteristics
US6436675B1 (en) 1999-09-28 2002-08-20 Maxygen, Inc. Use of codon-varied oligonucleotide synthesis for synthetic shuffling
US6961664B2 (en) 1999-01-19 2005-11-01 Maxygen Methods of populating data structures for use in evolutionary simulations
JP2002534966A (ja) 1999-01-19 2002-10-22 マキシジェン， インコーポレイテッド オリゴヌクレオチド媒介核酸組換え
US7024312B1 (en) 1999-01-19 2006-04-04 Maxygen, Inc. Methods for making character strings, polynucleotides and polypeptides having desired characteristics
JP2003524394A (ja) 1999-02-11 2003-08-19 マキシジェン， インコーポレイテッド ハイスループット質量分析法
US6531316B1 (en) 1999-03-05 2003-03-11 Maxyag, Inc. Encryption of traits using split gene sequences and engineered genetic elements
AU3391900A (en) 1999-03-05 2000-09-21 Maxygen, Inc. Encryption of traits using split gene sequences
US7430477B2 (en) 1999-10-12 2008-09-30 Maxygen, Inc. Methods of populating data structures for use in evolutionary simulations
US6686515B1 (en) 1999-11-23 2004-02-03 Maxygen, Inc. Homologous recombination in plants
US7115712B1 (en) 1999-12-02 2006-10-03 Maxygen, Inc. Cytokine polypeptides
EP1276900A2 (en) 2000-01-11 2003-01-22 Maxygen, Inc. Integrated systems and methods for diversity generation and screening
EP1297146A2 (en) 2000-06-23 2003-04-02 Maxygen, Inc. Novel chimeric promoters
JP2004513878A (ja) 2000-06-23 2004-05-13 マキシジェン，　インコーポレイテッド 新規同時刺激分子
US6858422B2 (en) 2000-07-13 2005-02-22 Codexis, Inc. Lipase genes
JP4647117B2 (ja) * 2001-03-07 2011-03-09 セレスター・レキシコ・サイエンシズ株式会社 突然変異誘発方法
WO2004020627A2 (en) * 2002-08-29 2004-03-11 Andreas Beck Improved dna and proteins
DE102005037351B3 (de) * 2005-08-08 2007-01-11 Geneart Ag Verfahren für die kontinuierliche zielgerichtete Evolution von Proteinen in vitro
US20090269803A1 (en) * 2005-09-21 2009-10-29 Andreas Meyer In Vivo Generation of Dna, Rna, Peptide, and Protein Libraries
KR101098032B1 (ko) * 2008-04-22 2011-12-23 한국생명공학연구원 4차 연속 피씨알, 4차 블록 피씨알, 또는 유전자 합성방법을 이용한 균주 특이적 바코드를 포함하는 유전자 적중 이형접합체 분열효모 균주의 제조방법
CN101532181B (zh) * 2009-03-13 2011-07-20 南京仙奕基因科技有限公司 原位构建基因突变文库的方法及试剂盒
SG11201506696TA (en) * 2013-02-26 2015-09-29 Axiomx Inc Methods for the production of libraries for directed evolution
CN110295159A (zh) * 2018-03-07 2019-10-01 江南大学 一种酶突变体
CN111574633B (zh) * 2020-05-15 2022-04-08 清华大学 一种具有广谱抗病毒功能的蛋白质CbAE的应用
CN114921440B (zh) * 2022-05-16 2023-05-05 北京昕大洋科技发展有限公司 一种木聚糖酶突变体及其应用

Family Cites Families (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
FR2641793B1 (fr) * 1988-12-26 1993-10-01 Setratech Procede de recombinaison in vivo de sequences d'adn presentant des mesappariements de bases

Also Published As

Publication number Publication date
CN101070537A (zh) 2007-11-14
DE69733059T2 (de) 2006-03-02
CN1210557A (zh) 1999-03-10
EP0873398B1 (en) 2005-04-20
DE69733059D1 (de) 2005-05-25
JP2000502568A (ja) 2000-03-07
EP0873398A1 (en) 1998-10-28
ATE293684T1 (de) 2005-05-15
AU1367897A (en) 1997-08-01
WO1997025410A1 (en) 1997-07-17

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