CN120796440A - 核酸分离及相关方法 - Google Patents

核酸分离及相关方法

Info

Publication number
CN120796440A
CN120796440A CN202511031238.5A CN202511031238A CN120796440A CN 120796440 A CN120796440 A CN 120796440A CN 202511031238 A CN202511031238 A CN 202511031238A CN 120796440 A CN120796440 A CN 120796440A
Authority
CN
China
Prior art keywords
nucleic acid
solid support
pectin
sample
amidated
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Pending
Application number
CN202511031238.5A
Other languages
English (en)
Chinese (zh)
Inventor
A·I·库蒂亚文
O·Z·纳纳西
D·塞尔盖耶夫
A·A·高尔
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Cepheid
Original Assignee
Cepheid
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Cepheid filed Critical Cepheid
Publication of CN120796440A publication Critical patent/CN120796440A/zh
Pending legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6806—Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1003—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor
    • C12N15/1006—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor by means of a solid support carrier, e.g. particles, polymers
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/686—Polymerase chain reaction [PCR]

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Genetics & Genomics (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Analytical Chemistry (AREA)
  • Biomedical Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Molecular Biology (AREA)
  • Biophysics (AREA)
  • Biochemistry (AREA)
  • Physics & Mathematics (AREA)
  • Microbiology (AREA)
  • General Health & Medical Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Immunology (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Plant Pathology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Solid-Sorbent Or Filter-Aiding Compositions (AREA)
  • Treatment Of Liquids With Adsorbents In General (AREA)
  • Polysaccharides And Polysaccharide Derivatives (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
CN202511031238.5A 2018-08-17 2019-08-16 核酸分离及相关方法 Pending CN120796440A (zh)

Applications Claiming Priority (4)

Application Number Priority Date Filing Date Title
US201862765149P 2018-08-17 2018-08-17
US62/765149 2018-08-17
CN201980065458.6A CN113015800A (zh) 2018-08-17 2019-08-16 核酸分离及相关方法
PCT/US2019/046912 WO2020037262A1 (en) 2018-08-17 2019-08-16 Nucleic acid isolation and related methods

Related Parent Applications (1)

Application Number Title Priority Date Filing Date
CN201980065458.6A Division CN113015800A (zh) 2018-08-17 2019-08-16 核酸分离及相关方法

Publications (1)

Publication Number Publication Date
CN120796440A true CN120796440A (zh) 2025-10-17

Family

ID=67809722

Family Applications (2)

Application Number Title Priority Date Filing Date
CN202511031238.5A Pending CN120796440A (zh) 2018-08-17 2019-08-16 核酸分离及相关方法
CN201980065458.6A Pending CN113015800A (zh) 2018-08-17 2019-08-16 核酸分离及相关方法

Family Applications After (1)

Application Number Title Priority Date Filing Date
CN201980065458.6A Pending CN113015800A (zh) 2018-08-17 2019-08-16 核酸分离及相关方法

Country Status (9)

Country Link
US (1) US20210238583A1 (https=)
EP (1) EP3837364A1 (https=)
JP (2) JP2021533778A (https=)
KR (1) KR20210045452A (https=)
CN (2) CN120796440A (https=)
AU (1) AU2019322911B2 (https=)
CA (1) CA3109814A1 (https=)
WO (1) WO2020037262A1 (https=)
ZA (1) ZA202101477B (https=)

Families Citing this family (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN113195714A (zh) * 2018-08-17 2021-07-30 塞弗德公司 核酸分离的方法和组合物
CN111607590B (zh) * 2020-06-08 2021-07-06 申翌生物科技(杭州)有限公司 适用于全血、血清或血浆的磁珠法核酸提取方法及试剂盒
CN115160452A (zh) * 2022-08-03 2022-10-11 广东纤友朵美生物科技有限公司 一种含活性醛基结构的高酯果胶及其制备方法

Family Cites Families (15)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
GB9927904D0 (en) * 1999-11-25 2000-01-26 Amersham Pharm Biotech Ab A method fro obtaining a nucleic acid variant
US6374684B1 (en) 2000-08-25 2002-04-23 Cepheid Fluid control and processing system
FR2843302B1 (fr) * 2002-08-09 2004-10-22 Centre Nat Rech Scient Forme galenique pour la delivrance colique de principes actifs
US20140011201A1 (en) * 2003-05-13 2014-01-09 Ibis Biosciences, Inc. Method for the purification of targeted nucleic acids from background nucleic acids
US8158354B2 (en) * 2003-05-13 2012-04-17 Ibis Biosciences, Inc. Methods for rapid purification of nucleic acids for subsequent analysis by mass spectrometry by solution capture
US20050106589A1 (en) * 2003-11-17 2005-05-19 Hashem Akhavan-Tafti Compositions and methods for releasing nucleic acids from solid phase binding materials
MXPA05001815A (es) * 2004-02-20 2005-08-24 Hoffmann La Roche Adsorcion de acidos nucleicos a una fase solida.
DE102008063003A1 (de) * 2008-12-23 2010-06-24 Qiagen Gmbh Nukleinsäureaufreinigungsverfahren
US8039613B2 (en) * 2009-08-28 2011-10-18 Promega Corporation Methods of purifying a nucleic acid and formulation and kit for use in performing such methods
EP2760999A1 (en) * 2011-09-26 2014-08-06 Qiagen GmbH Methods for separating nucleic acids by size
WO2015120445A1 (en) * 2014-02-10 2015-08-13 Nancy Quan Isolation of cell-free nucleic acids from bodily fluid samples using solid chaotropic agents
JP6787916B2 (ja) * 2015-06-10 2020-11-18 キアゲン ゲーエムベーハー アニオン交換粒子を使用する細胞外核酸を単離する方法
CN105131148A (zh) * 2015-09-07 2015-12-09 三门峡康瑞药业有限公司 酰胺化果胶的制备方法
FR3078977B1 (fr) * 2018-03-16 2023-03-17 Biomerieux Sa Procede d'extraction d'acides nucleiques
JP7431217B2 (ja) * 2018-08-17 2024-02-14 セファイド 核酸デコンタミネーション方法

Also Published As

Publication number Publication date
US20210238583A1 (en) 2021-08-05
JP7811612B2 (ja) 2026-02-05
ZA202101477B (en) 2024-08-28
JP2024129021A (ja) 2024-09-26
KR20210045452A (ko) 2021-04-26
JP2021533778A (ja) 2021-12-09
WO2020037262A1 (en) 2020-02-20
CA3109814A1 (en) 2020-02-20
AU2019322911A1 (en) 2021-04-08
AU2019322911B2 (en) 2025-12-18
CN113015800A (zh) 2021-06-22
EP3837364A1 (en) 2021-06-23

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