KR20210045452A - 핵산 단리 및 관련 방법 - Google Patents

핵산 단리 및 관련 방법 Download PDF

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Publication number
KR20210045452A
KR20210045452A KR1020217007885A KR20217007885A KR20210045452A KR 20210045452 A KR20210045452 A KR 20210045452A KR 1020217007885 A KR1020217007885 A KR 1020217007885A KR 20217007885 A KR20217007885 A KR 20217007885A KR 20210045452 A KR20210045452 A KR 20210045452A
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KR
South Korea
Prior art keywords
nucleic acid
solid support
pectin
amidated
sample
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Ceased
Application number
KR1020217007885A
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English (en)
Korean (ko)
Inventor
알렉스 아이. 쿠티아빈
올리버 제트. 나나시
드미트리 세르귀브
알렉산더 에이. 갈
Original Assignee
세페이드
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
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Publication date
Application filed by 세페이드 filed Critical 세페이드
Publication of KR20210045452A publication Critical patent/KR20210045452A/ko
Ceased legal-status Critical Current

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6806—Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1003—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor
    • C12N15/1006—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor by means of a solid support carrier, e.g. particles, polymers
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/686—Polymerase chain reaction [PCR]

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  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Genetics & Genomics (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Analytical Chemistry (AREA)
  • Biomedical Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Molecular Biology (AREA)
  • Biophysics (AREA)
  • Biochemistry (AREA)
  • Physics & Mathematics (AREA)
  • Microbiology (AREA)
  • General Health & Medical Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Immunology (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Plant Pathology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Solid-Sorbent Or Filter-Aiding Compositions (AREA)
  • Treatment Of Liquids With Adsorbents In General (AREA)
  • Polysaccharides And Polysaccharide Derivatives (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
KR1020217007885A 2018-08-17 2019-08-16 핵산 단리 및 관련 방법 Ceased KR20210045452A (ko)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US201862765149P 2018-08-17 2018-08-17
US62/765,149 2018-08-17
PCT/US2019/046912 WO2020037262A1 (en) 2018-08-17 2019-08-16 Nucleic acid isolation and related methods

Publications (1)

Publication Number Publication Date
KR20210045452A true KR20210045452A (ko) 2021-04-26

Family

ID=67809722

Family Applications (1)

Application Number Title Priority Date Filing Date
KR1020217007885A Ceased KR20210045452A (ko) 2018-08-17 2019-08-16 핵산 단리 및 관련 방법

Country Status (9)

Country Link
US (1) US20210238583A1 (https=)
EP (1) EP3837364A1 (https=)
JP (2) JP2021533778A (https=)
KR (1) KR20210045452A (https=)
CN (2) CN120796440A (https=)
AU (1) AU2019322911B2 (https=)
CA (1) CA3109814A1 (https=)
WO (1) WO2020037262A1 (https=)
ZA (1) ZA202101477B (https=)

Families Citing this family (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN113195714A (zh) * 2018-08-17 2021-07-30 塞弗德公司 核酸分离的方法和组合物
CN111607590B (zh) * 2020-06-08 2021-07-06 申翌生物科技(杭州)有限公司 适用于全血、血清或血浆的磁珠法核酸提取方法及试剂盒
CN115160452A (zh) * 2022-08-03 2022-10-11 广东纤友朵美生物科技有限公司 一种含活性醛基结构的高酯果胶及其制备方法

Family Cites Families (15)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
GB9927904D0 (en) * 1999-11-25 2000-01-26 Amersham Pharm Biotech Ab A method fro obtaining a nucleic acid variant
US6374684B1 (en) 2000-08-25 2002-04-23 Cepheid Fluid control and processing system
FR2843302B1 (fr) * 2002-08-09 2004-10-22 Centre Nat Rech Scient Forme galenique pour la delivrance colique de principes actifs
US20140011201A1 (en) * 2003-05-13 2014-01-09 Ibis Biosciences, Inc. Method for the purification of targeted nucleic acids from background nucleic acids
US8158354B2 (en) * 2003-05-13 2012-04-17 Ibis Biosciences, Inc. Methods for rapid purification of nucleic acids for subsequent analysis by mass spectrometry by solution capture
US20050106589A1 (en) * 2003-11-17 2005-05-19 Hashem Akhavan-Tafti Compositions and methods for releasing nucleic acids from solid phase binding materials
MXPA05001815A (es) * 2004-02-20 2005-08-24 Hoffmann La Roche Adsorcion de acidos nucleicos a una fase solida.
DE102008063003A1 (de) * 2008-12-23 2010-06-24 Qiagen Gmbh Nukleinsäureaufreinigungsverfahren
US8039613B2 (en) * 2009-08-28 2011-10-18 Promega Corporation Methods of purifying a nucleic acid and formulation and kit for use in performing such methods
EP2760999A1 (en) * 2011-09-26 2014-08-06 Qiagen GmbH Methods for separating nucleic acids by size
WO2015120445A1 (en) * 2014-02-10 2015-08-13 Nancy Quan Isolation of cell-free nucleic acids from bodily fluid samples using solid chaotropic agents
JP6787916B2 (ja) * 2015-06-10 2020-11-18 キアゲン ゲーエムベーハー アニオン交換粒子を使用する細胞外核酸を単離する方法
CN105131148A (zh) * 2015-09-07 2015-12-09 三门峡康瑞药业有限公司 酰胺化果胶的制备方法
FR3078977B1 (fr) * 2018-03-16 2023-03-17 Biomerieux Sa Procede d'extraction d'acides nucleiques
JP7431217B2 (ja) * 2018-08-17 2024-02-14 セファイド 核酸デコンタミネーション方法

Also Published As

Publication number Publication date
US20210238583A1 (en) 2021-08-05
CN120796440A (zh) 2025-10-17
JP7811612B2 (ja) 2026-02-05
ZA202101477B (en) 2024-08-28
JP2024129021A (ja) 2024-09-26
JP2021533778A (ja) 2021-12-09
WO2020037262A1 (en) 2020-02-20
CA3109814A1 (en) 2020-02-20
AU2019322911A1 (en) 2021-04-08
AU2019322911B2 (en) 2025-12-18
CN113015800A (zh) 2021-06-22
EP3837364A1 (en) 2021-06-23

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