CN113015800A - 核酸分离及相关方法 - Google Patents

核酸分离及相关方法 Download PDF

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Publication number
CN113015800A
CN113015800A CN201980065458.6A CN201980065458A CN113015800A CN 113015800 A CN113015800 A CN 113015800A CN 201980065458 A CN201980065458 A CN 201980065458A CN 113015800 A CN113015800 A CN 113015800A
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CN
China
Prior art keywords
solid support
nucleic acid
pectin
amidated
sample
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Pending
Application number
CN201980065458.6A
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English (en)
Chinese (zh)
Inventor
A·I·库蒂亚文
O·Z·纳纳西
D·塞尔盖耶夫
A·A·高尔
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Cepheid
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Cepheid
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Publication date
Application filed by Cepheid filed Critical Cepheid
Priority to CN202511031238.5A priority Critical patent/CN120796440A/zh
Publication of CN113015800A publication Critical patent/CN113015800A/zh
Pending legal-status Critical Current

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6806—Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1003—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor
    • C12N15/1006—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor by means of a solid support carrier, e.g. particles, polymers
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/686—Polymerase chain reaction [PCR]

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  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Genetics & Genomics (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Analytical Chemistry (AREA)
  • Biomedical Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Molecular Biology (AREA)
  • Biophysics (AREA)
  • Biochemistry (AREA)
  • Physics & Mathematics (AREA)
  • Microbiology (AREA)
  • General Health & Medical Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Immunology (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Plant Pathology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Treatment Of Liquids With Adsorbents In General (AREA)
  • Solid-Sorbent Or Filter-Aiding Compositions (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
  • Polysaccharides And Polysaccharide Derivatives (AREA)
CN201980065458.6A 2018-08-17 2019-08-16 核酸分离及相关方法 Pending CN113015800A (zh)

Priority Applications (1)

Application Number Priority Date Filing Date Title
CN202511031238.5A CN120796440A (zh) 2018-08-17 2019-08-16 核酸分离及相关方法

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US201862765149P 2018-08-17 2018-08-17
US62/765149 2018-08-17
PCT/US2019/046912 WO2020037262A1 (en) 2018-08-17 2019-08-16 Nucleic acid isolation and related methods

Related Child Applications (1)

Application Number Title Priority Date Filing Date
CN202511031238.5A Division CN120796440A (zh) 2018-08-17 2019-08-16 核酸分离及相关方法

Publications (1)

Publication Number Publication Date
CN113015800A true CN113015800A (zh) 2021-06-22

Family

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Family Applications (2)

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CN201980065458.6A Pending CN113015800A (zh) 2018-08-17 2019-08-16 核酸分离及相关方法
CN202511031238.5A Pending CN120796440A (zh) 2018-08-17 2019-08-16 核酸分离及相关方法

Family Applications After (1)

Application Number Title Priority Date Filing Date
CN202511031238.5A Pending CN120796440A (zh) 2018-08-17 2019-08-16 核酸分离及相关方法

Country Status (9)

Country Link
US (1) US20210238583A1 (https=)
EP (1) EP3837364A1 (https=)
JP (2) JP2021533778A (https=)
KR (1) KR20210045452A (https=)
CN (2) CN113015800A (https=)
AU (1) AU2019322911B2 (https=)
CA (1) CA3109814A1 (https=)
WO (1) WO2020037262A1 (https=)
ZA (1) ZA202101477B (https=)

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN113195714A (zh) * 2018-08-17 2021-07-30 塞弗德公司 核酸分离的方法和组合物
CN115160452A (zh) * 2022-08-03 2022-10-11 广东纤友朵美生物科技有限公司 一种含活性醛基结构的高酯果胶及其制备方法

Families Citing this family (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN111607590B (zh) * 2020-06-08 2021-07-06 申翌生物科技(杭州)有限公司 适用于全血、血清或血浆的磁珠法核酸提取方法及试剂盒

Citations (8)

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US20050130196A1 (en) * 2003-05-13 2005-06-16 Hofstadler Steven A. Methods for rapid purification of nucleic acids for subsequent analysis by mass spectrometry by solution capture
CN1680421A (zh) * 2004-02-20 2005-10-12 霍夫曼-拉罗奇有限公司 在核酸固相上的吸附
CN101018870A (zh) * 2004-07-15 2007-08-15 鲁米根公司 使用可裂解固相分离核酸的方法
US20110054161A1 (en) * 2009-08-28 2011-03-03 Promega Corporation Methods of purifying a nucleic acid and formulation and kit for use in performing such methods
US20140011201A1 (en) * 2003-05-13 2014-01-09 Ibis Biosciences, Inc. Method for the purification of targeted nucleic acids from background nucleic acids
CN107690481A (zh) * 2015-06-10 2018-02-13 凯杰有限公司 利用阴离子交换粒子分离细胞外核酸的方法
CN111868253A (zh) * 2018-03-16 2020-10-30 生物梅里埃公司 提取核酸的方法
CN113056291A (zh) * 2018-08-17 2021-06-29 塞弗德公司 核酸去污方法

Family Cites Families (7)

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GB9927904D0 (en) * 1999-11-25 2000-01-26 Amersham Pharm Biotech Ab A method fro obtaining a nucleic acid variant
US6374684B1 (en) 2000-08-25 2002-04-23 Cepheid Fluid control and processing system
FR2843302B1 (fr) * 2002-08-09 2004-10-22 Centre Nat Rech Scient Forme galenique pour la delivrance colique de principes actifs
DE102008063003A1 (de) * 2008-12-23 2010-06-24 Qiagen Gmbh Nukleinsäureaufreinigungsverfahren
US20140243216A1 (en) * 2011-09-26 2014-08-28 Qiagen Gmbh Methods for separating nucleic acids by size
US10619152B2 (en) * 2014-02-10 2020-04-14 Nancy Quan Isolation of cell-free nucleic acids from bodily fluid samples using solid chaotropic agents
CN105131148A (zh) * 2015-09-07 2015-12-09 三门峡康瑞药业有限公司 酰胺化果胶的制备方法

Patent Citations (10)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20050130196A1 (en) * 2003-05-13 2005-06-16 Hofstadler Steven A. Methods for rapid purification of nucleic acids for subsequent analysis by mass spectrometry by solution capture
US20140011201A1 (en) * 2003-05-13 2014-01-09 Ibis Biosciences, Inc. Method for the purification of targeted nucleic acids from background nucleic acids
CN1680421A (zh) * 2004-02-20 2005-10-12 霍夫曼-拉罗奇有限公司 在核酸固相上的吸附
CN101018870A (zh) * 2004-07-15 2007-08-15 鲁米根公司 使用可裂解固相分离核酸的方法
US20110054161A1 (en) * 2009-08-28 2011-03-03 Promega Corporation Methods of purifying a nucleic acid and formulation and kit for use in performing such methods
JP2013502934A (ja) * 2009-08-28 2013-01-31 プロメガ コーポレイション 核酸の精製方法及びこの方法の実施に使用する配合物及びキット
CN107690481A (zh) * 2015-06-10 2018-02-13 凯杰有限公司 利用阴离子交换粒子分离细胞外核酸的方法
JP2018518150A (ja) * 2015-06-10 2018-07-12 キアゲン ゲーエムベーハー アニオン交換粒子を使用する細胞外核酸を単離する方法
CN111868253A (zh) * 2018-03-16 2020-10-30 生物梅里埃公司 提取核酸的方法
CN113056291A (zh) * 2018-08-17 2021-06-29 塞弗德公司 核酸去污方法

Non-Patent Citations (1)

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Title
MARCELLO LENUCCI ET AL.: "" Do polyamines contribute to plant cell wall assembly by forming amide bonds with pectins?"", 《PHYTOCHEMISTRY》, vol. 12, 1 June 2005 (2005-06-01), pages 2583 *

Cited By (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN113195714A (zh) * 2018-08-17 2021-07-30 塞弗德公司 核酸分离的方法和组合物
US12365891B2 (en) 2018-08-17 2025-07-22 Cepheid Methods and compositions for nucleic acid isolation
CN115160452A (zh) * 2022-08-03 2022-10-11 广东纤友朵美生物科技有限公司 一种含活性醛基结构的高酯果胶及其制备方法

Also Published As

Publication number Publication date
KR20210045452A (ko) 2021-04-26
WO2020037262A1 (en) 2020-02-20
AU2019322911A1 (en) 2021-04-08
JP2024129021A (ja) 2024-09-26
EP3837364A1 (en) 2021-06-23
ZA202101477B (en) 2024-08-28
US20210238583A1 (en) 2021-08-05
CA3109814A1 (en) 2020-02-20
AU2019322911B2 (en) 2025-12-18
JP2021533778A (ja) 2021-12-09
CN120796440A (zh) 2025-10-17
JP7811612B2 (ja) 2026-02-05

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