CN117551563A - 一种复合菌糖肽制造工艺及液体培养基和固体培养基的制备方法 - Google Patents
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Abstract
本发明提供了一种制造复合菌糖肽的液体培养基的制备方法,包括如下步骤:将土豆洗净去皮切片,加入麸皮,适量的水,至煮沸20分钟后过滤;加入蔗糖、MgSO4,KH2PO4,混均,灭菌。本发明涉及一种复合菌糖肽制造工艺,包括如下步骤:制作液体培养基,灭菌后的液体培养基接种灵芝、蛹虫草、茯苓等,制作固体培养基,酶解,烘干后粉碎至细末状,得到含有真菌多糖和真菌多肽的产品。本发明将传统的食用的粮食淀粉通过食用菌的发酵、酶解,将淀粉转化成对机体具有调节生理活性的菌多糖,将食用的粮食的蛋白质通过食用菌的发酵、酶解,将粮食蛋白转化成对机体易吸收、具有调节生理活性的多肽,制造工艺简单、实施成本低廉、转化效率高。
Description
本申请是申请日为2015年07月08日、申请号为201510395278.8、发明名称为《一种复合菌糖肽制造工艺》的分案申请。
技术领域
本发明涉及一种复合菌糖肽制造工艺及液体培养基和固体培养基的制备方法。
背景技术
在公知的技术领域,糖肽是糖蛋白和蛋白聚糖中寡糖与氨基酸或多肽链共价连接而形成的结构区域,是生物体内极为重要的生物信息分子。在细胞分裂、信号转导、细胞识别、癌症、免疫、炎症、微生物感染等众多的生命现象和疾病形成过程中,糖肽均扮演着非常重要的角色。研究糖肽的结构与功能之间的关系对于揭示其生物学功能以及糖肽类药物的开发有重要作用。由于生物体内的糖肽含量低且具有微不均一性,从生物样品中分离纯化糖肽十分困难,常常需要合成糖肽作为模型分子来研究其构效关系,因此糖肽合成方法的研究成为糖生物学和糖化学领域的一大热点。
现有技术中,真菌培养基包括以玉米浆、玉米皮、玉米粉、大米粉、面粉、麦麸、土豆、葡萄糖和CMC中的一种或多种为碳源,以豆粕、动物蛋白、植物蛋白和硫铵等作为氮源,一般除了含碳物质还需要配以优质含氮物质,成本较高,因此亟待提供一种成本低廉的复合菌培养基。
发明内容
本发明要解决的技术问题是:提供一种以土豆为碳源,转化效率高、简单方便且制造成本低廉的复合菌糖肽制造工艺。
本发明解决其技术问题所采用的技术方案是:
本发明提供了一种制造复合菌糖肽的液体培养基的制备方法,包括如下步骤:
(1)制作液体培养基,将土豆洗净,称取所需液体培养基总质量的30%去皮切片,称取总质量7%的麸皮,加入适量的水,至煮沸20分钟后过滤,取过滤液并补足体积;
(2)称取总质量2%的蔗糖、0.015%的MgSO4,0.075%的KH2PO4,混合搅拌均匀,装入抽滤瓶;
(3)高压锅灭菌30分钟,温度保持在121℃,得到液体培养基。
本发明提供了一种制造复合菌糖肽的固体培养基的制备方法,包括如下步骤:
(1)制作固体培养基,将玉米、莜麦、大豆和小麦粉碎后按质量比7:1:1:1混合,加入总质量55%的水、3%的蔗糖、0.03%的MgSO4、0.06%的KH2PO4、1%的CaCO4,并按照每100g混合液体添加0.035mg的硒,搅拌均匀装入培养瓶;
(2)固体培养瓶高压锅灭菌1.5h,温度121℃,得到固体培养基。
本发明还提供了一种复合菌糖肽制造工艺,包括如下步骤:
(a)制作液体培养基,将土豆洗净,称取所需液体培养基总质量的30%去皮切片,称取总质量7%的麸皮,加入适量的水,至煮沸20分钟后过滤,取过滤液并补足体积;
(b)称取总质量2%的蔗糖、0.015%的MgSO4,0.075%的KH2PO4,混合搅拌均匀,装入抽滤瓶;
(c)高压锅灭菌30分钟,温度保持在121℃,得到液体培养基;
(d)灭菌后的液体培养基接种灵芝、蛹虫草、茯苓、灰树花、云芝、猴头菌,培养5~7天,混合后得到液体菌种;
(e)制作固体培养基,将玉米、莜麦、大豆和小麦粉碎后按质量比7:1:1:1混合,加入总质量55%的水、3%的蔗糖、0.03%的MgSO4、0.06%的KH2PO4、1%的CaCO4,并按照每100g混合液体添加0.035mg的硒,搅拌均匀装入培养瓶;
(f)固体培养瓶高压锅灭菌1.5h,温度121℃,得到固体培养基;
(g)接种室内用空气消毒杀菌机灭菌30分钟;
(h)将液体菌种接种到培养瓶中,28℃下培养10~15天;
(i)取出培养物烘干,膨化粉碎,得到菌粉;
(j)菌粉与水以质量比3:20的比例混合搅拌均匀;
(k)加入适量蛋白酶,淀粉酶,果胶酶,糖化酶及纤维素酶混合均匀,50℃~55℃下恒温搅拌24h酶解;
(1)酶解液在80℃下灭活20分钟;
(m)烘干后粉碎至细末状,得到含有真菌多糖和真菌多肽的产品。
进一步的,本发明中所获得真菌多糖与真菌多肽试质量比为4:1。
本发明的有益效果是:本发明将传统的食用的粮食淀粉通过食用菌的发酵、酶解,将淀粉转化成对机体具有调节生理活性的菌多糖,将食用的粮食的蛋白质通过食用菌的发酵、酶解,将粮食蛋白转化成对机体易吸收、具有调节生理活性的多肽,制造工艺简单、实施成本低廉、转化效率高。
具体实施方式
本发明提供了一种复合菌糖肽制造工艺,包括如下步骤:
(a)制作液体培养基,将土豆洗净,称取所需液体培养基总质量的30%去皮切片,称取总质量7%的麸皮,加入适量的水,至煮沸20分钟后过滤,取过滤液并补足体积;
(b)称取总质量2%的蔗糖、0.015%的MgSO4,0.075%的KH2PO4,混合搅拌均匀,装入抽滤瓶;
(c)高压锅灭菌30分钟,温度保持在121℃,得到液体培养基;
(d)灭菌后的液体培养基接种灵芝、蛹虫草、茯苓、灰树花、云芝、猴头菌,培养5~7天,混合后得到液体菌种;
(e)制作固体培养基,将玉米、莜麦、大豆和小麦粉碎后按质量比7:1:1:1混合,加入总质量55%的水、3%的蔗糖、0.03%的MgSO4、0.06%的KH2PO4、1%的CaCO4,并按照每100g混合液体添加0.035mg的硒,搅拌均匀装入培养瓶;
(f)固体培养瓶高压锅灭菌1.5h,温度121℃,得到固体培养基;
(g)接种室内用空气消毒杀菌机灭菌30分钟;
(h)将液体菌种接种到培养瓶中,28℃下培养10~15天;
(i)取出培养物烘干,膨化粉碎,得到菌粉;
(j)菌粉与水以质量比3:20的比例混合搅拌均匀;
(k)加入适量蛋白酶,淀粉酶,果胶酶,糖化酶及纤维素酶混合均匀,50℃~55℃下恒温搅拌24h酶解;
(1)酶解液在80℃下灭活20分钟;
(m)烘干后粉碎至细末状,得到含有真菌多糖和真菌多肽的产品,所获得真菌多糖与真菌多肽试质量比为4:1。
以上述依据本发明的理想实施例为启示,通过上述的说明内容,相关工作人员完全可以在不偏离本项发明技术思想的范围内,进行多样的变更以及修改。本项发明的技术性范围并不局限于说明书上的内容,必须要根据权利要求范围来确定其技术性范围。
Claims (4)
1.一种制造复合菌糖肽的液体培养基的制备方法,其特征在于,包括如下步骤:
(1)制作液体培养基,将土豆洗净,称取所需液体培养基总质量的30%去皮切片,称取总质量7%的麸皮,加入适量的水,至煮沸20分钟后过滤,取过滤液并补足体积;
(2)称取总质量2%的蔗糖、0.015%的MgSO4,0.075%的KH2PO4,混合搅拌均匀,装入抽滤瓶;
(3)高压锅灭菌30分钟,温度保持在121℃,得到液体培养基。
2.一种制造复合菌糖肽的固体培养基的制备方法,其特征在于,包括如下步骤:
(1)制作固体培养基,将玉米、莜麦、大豆和小麦粉碎后按质量比7:1:1:1混合,加入总质量55%的水、3%的蔗糖、0.03%的MgSO4、0.06%的KH2PO4、1%的CaCO4,并按照每100g混合液体添加0.035mg的硒,搅拌均匀装入培养瓶;
(2)固体培养瓶高压锅灭菌1.5h,温度121℃,得到固体培养基。
3.一种复合菌糖肽制造工艺,其特征在于,包括如下步骤:
(a)制作液体培养基,将土豆洗净,称取所需液体培养基总质量的30%去皮切片,称取总质量7%的麸皮,加入适量的水,至煮沸20分钟后过滤,取过滤液并补足体积;
(b)称取总质量2%的蔗糖、0.015%的MgSO4,0.075%的KH2PO4,混合搅拌均匀,装入抽滤瓶;
(c)高压锅灭菌30分钟,温度保持在121℃,得到液体培养基;
(d)灭菌后的液体培养基接种灵芝、蛹虫草、茯苓、灰树花、云芝、猴头菌,培养5~7天,混合后得到液体菌种;
(e)制作固体培养基,将玉米、莜麦、大豆和小麦粉碎后按质量比7:1:1:1混合,加入总质量55%的水、3%的蔗糖、0.03%的MgSO4、0.06%的KH2PO4、1%的CaCO4,并按照每100g混合液体添加0.035mg的硒,搅拌均匀装入培养瓶;
(f)固体培养瓶高压锅灭菌1.5h,温度121℃;得到固体培养基;
(g)接种室内用空气消毒杀菌机灭菌30分钟;
(h)将液体菌种接种到培养瓶中,28℃下培养10~15天;
(i)取出培养物烘干,膨化粉碎,得到菌粉;
(j)菌粉与水以质量比3:20的比例混合搅拌均匀;
(k)加入适量蛋白酶,淀粉酶,果胶酶,糖化酶及纤维素酶混合均匀,50℃~55℃下恒温搅拌24h酶解;
(1)酶解液在80℃下灭活20分钟;
(m)烘干后粉碎至细末状,得到含有真菌多糖和真菌多肽的产品。
4.如权利要求3所述的一种复合菌糖肽制造工艺,其特征在于,所获得真菌多糖与真菌多肽试质量比为4:1。
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