CN116076252A - Precise application method of exogenous substances for strong seedling cultivation of grafted seedlings - Google Patents
Precise application method of exogenous substances for strong seedling cultivation of grafted seedlings Download PDFInfo
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- CN116076252A CN116076252A CN202211502015.9A CN202211502015A CN116076252A CN 116076252 A CN116076252 A CN 116076252A CN 202211502015 A CN202211502015 A CN 202211502015A CN 116076252 A CN116076252 A CN 116076252A
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- cotyledon
- grafting
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- scion
- stock
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- 238000000034 method Methods 0.000 title claims abstract description 36
- 239000000126 substance Substances 0.000 title claims abstract description 33
- 230000035876 healing Effects 0.000 claims abstract description 10
- 239000003153 chemical reaction reagent Substances 0.000 claims abstract description 9
- 238000005728 strengthening Methods 0.000 claims abstract description 6
- 239000000463 material Substances 0.000 claims description 17
- 229930192334 Auxin Natural products 0.000 claims description 11
- 239000002363 auxin Substances 0.000 claims description 11
- SEOVTRFCIGRIMH-UHFFFAOYSA-N indole-3-acetic acid Chemical compound C1=CC=C2C(CC(=O)O)=CNC2=C1 SEOVTRFCIGRIMH-UHFFFAOYSA-N 0.000 claims description 11
- 239000007788 liquid Substances 0.000 claims description 8
- 210000002615 epidermis Anatomy 0.000 claims description 7
- 238000006748 scratching Methods 0.000 claims 1
- 230000002393 scratching effect Effects 0.000 claims 1
- 239000013003 healing agent Substances 0.000 abstract description 4
- 238000004519 manufacturing process Methods 0.000 abstract description 2
- 235000013311 vegetables Nutrition 0.000 abstract description 2
- 238000009423 ventilation Methods 0.000 description 6
- 238000002791 soaking Methods 0.000 description 5
- 241000219112 Cucumis Species 0.000 description 4
- 235000015510 Cucumis melo subsp melo Nutrition 0.000 description 4
- 230000003203 everyday effect Effects 0.000 description 4
- 239000002985 plastic film Substances 0.000 description 4
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 4
- 241000196324 Embryophyta Species 0.000 description 3
- FJJCIZWZNKZHII-UHFFFAOYSA-N [4,6-bis(cyanoamino)-1,3,5-triazin-2-yl]cyanamide Chemical compound N#CNC1=NC(NC#N)=NC(NC#N)=N1 FJJCIZWZNKZHII-UHFFFAOYSA-N 0.000 description 3
- 238000005520 cutting process Methods 0.000 description 3
- 238000007598 dipping method Methods 0.000 description 3
- 230000000694 effects Effects 0.000 description 3
- 235000000832 Ayote Nutrition 0.000 description 2
- 235000009854 Cucurbita moschata Nutrition 0.000 description 2
- 240000001980 Cucurbita pepo Species 0.000 description 2
- 235000009804 Cucurbita pepo subsp pepo Nutrition 0.000 description 2
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 description 2
- XEEYBQQBJWHFJM-UHFFFAOYSA-N Iron Chemical compound [Fe] XEEYBQQBJWHFJM-UHFFFAOYSA-N 0.000 description 2
- 239000005708 Sodium hypochlorite Substances 0.000 description 2
- 238000004140 cleaning Methods 0.000 description 2
- 230000008020 evaporation Effects 0.000 description 2
- 238000001704 evaporation Methods 0.000 description 2
- 230000002349 favourable effect Effects 0.000 description 2
- 230000035784 germination Effects 0.000 description 2
- 235000015097 nutrients Nutrition 0.000 description 2
- 229920003023 plastic Polymers 0.000 description 2
- 229920006255 plastic film Polymers 0.000 description 2
- 235000015136 pumpkin Nutrition 0.000 description 2
- SUKJFIGYRHOWBL-UHFFFAOYSA-N sodium hypochlorite Chemical compound [Na+].Cl[O-] SUKJFIGYRHOWBL-UHFFFAOYSA-N 0.000 description 2
- 238000005507 spraying Methods 0.000 description 2
- 230000001954 sterilising effect Effects 0.000 description 2
- 239000008399 tap water Substances 0.000 description 2
- 235000020679 tap water Nutrition 0.000 description 2
- 150000001720 carbohydrates Chemical class 0.000 description 1
- 230000007547 defect Effects 0.000 description 1
- 230000018109 developmental process Effects 0.000 description 1
- 239000012153 distilled water Substances 0.000 description 1
- 229910052742 iron Inorganic materials 0.000 description 1
- 230000002503 metabolic effect Effects 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 230000008092 positive effect Effects 0.000 description 1
- 238000002360 preparation method Methods 0.000 description 1
- 230000035945 sensitivity Effects 0.000 description 1
- 238000007619 statistical method Methods 0.000 description 1
- 238000004659 sterilization and disinfection Methods 0.000 description 1
Images
Classifications
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G2/00—Vegetative propagation
- A01G2/30—Grafting
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G7/00—Botany in general
- A01G7/06—Treatment of growing trees or plants, e.g. for preventing decay of wood, for tingeing flowers or wood, for prolonging the life of plants
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- Life Sciences & Earth Sciences (AREA)
- Botany (AREA)
- Environmental Sciences (AREA)
- Biodiversity & Conservation Biology (AREA)
- Ecology (AREA)
- Forests & Forestry (AREA)
- Developmental Biology & Embryology (AREA)
- Engineering & Computer Science (AREA)
- Wood Science & Technology (AREA)
- Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
Abstract
The invention belongs to the technical field of vegetable production, and particularly relates to a precise application method of exogenous substances for strong seedling cultivation of grafted seedlings, which comprises the following steps of (1) raising seedlings of scions and stocks; (2) grafting scions with the stock; (3) precise application of exogenous substances; and (4) managing the grafting healing period. By the method, exogenous substances can be accurately applied to the scions and the stocks, so that the action positions of the exogenous substances are judged, and development of grafting seedling strengthening reagents such as grafting healing agents special for the scions is facilitated.
Description
Technical Field
The invention belongs to the technical field of vegetable production, and particularly relates to a precise application method of exogenous substances for strong seedling cultivation of grafted seedlings.
Background
The application of chemical substances from external sources is one of the common means for improving the quality of grafted seedlings. The application of exogenous substances such as auxin, saccharides and the like can obviously improve the quality of grafted seedlings. However, the conventional application methods of exogenous substances mainly include dipping, soaking, smearing and spraying of whole plants on grafting cuts. The grafting wound is easily polluted by the modes of dipping, soaking, smearing and the like which are directly contacted with the wound, and a large amount of reagent is wasted by the modes of whole plant spraying and the like; simultaneously, the application of the mode enables the scion and the stock to be simultaneously attached with the exogenous reagent, and the main action parts which cannot effectively distinguish exogenous substances are the scion and the stock or both. The scion (lacking the underground part) and the stock (lacking the underground part) play roles in the grafting healing process, and have great differences in metabolic levels, and have different sensitivity degrees to the types and the concentrations of the exogenous substances, so that proper exogenous substances are required to be screened for the scion and the stock respectively, and technical guidance is provided for developing grafting seedling strengthening reagents such as grafting healing agents special for the scion. Therefore, the patent develops a precise application method of exogenous substances for strong seedling cultivation of grafted seedlings, which can effectively and respectively precisely apply exogenous substances to scions and stocks, further judge whether the action part of the exogenous substances is a scion or a stock or both, and is favorable for developing grafting seedling strengthening reagents such as grafting healing agents special for the scions. If the method of injecting the nutrient solution to the grafting position by adopting the needle tube of the prior art CN104303851A, namely adding the nutrient solution into the needle tube along the wound, is the same as the defect of soaking and dipping, the method of directly contacting the wound is easy to pollute the grafting wound, simultaneously, exogenous reagents are simultaneously attached to the scion and the stock, and the main action positions of the exogenous substances which cannot be effectively distinguished are the scion and the stock or both.
Disclosure of Invention
Aiming at the problems existing in the prior art, the invention provides a precise application method of exogenous substances for strong seedling cultivation of grafted seedlings, which aims to solve part of the problems in the prior art or at least relieve part of the problems in the prior art.
A precise application method of exogenous substances for strong seedling cultivation of grafted seedlings comprises the following steps:
(1) Seedling raising of scions and stocks;
(2) Grafting scions and stock;
(3) Accurately applying exogenous substances;
(4) Management of grafting healing period:
further, in the step (1), 5% sodium hypochlorite sterilization is carried out on scions and stock seeds for 10min, tap water is used for cleaning, and soaking is carried out for 6-8h at room temperature; accelerating germination at a constant temperature of 30 ℃ until radicle is exposed to white, and broadcasting the radicle in a flat disc; grafting the 1 st true leaves when the leaves are slightly exposed.
Further, in the step (2), monocotyledonous sticking is adopted, the scion is obliquely inclined at 30 degrees from the position 1cm below the basal part of the cotyledon, and the section is smooth; the stock is obliquely cut at an angle of 30 degrees at the basal part of cotyledons, a cotyledon and all growing points are cut off, and the section is smooth; and tightly attaching the stock to the cutting surface of the scion, and fixing the grafting clip.
Further, the blades and grafting clips used in the step (2) are sterilized by 75% medical alcohol in advance, and are naturally dried for use.
Further, the precise application of the exogenous material in the step (3) may be performed by the following procedure: 1ml of a medical syringe was taken. The needle point is used for sliding slightly on the back of the cotyledon of the scion or the stock, a lower epidermis wound with the length of about 2-3mm is formed, but the cotyledon cannot be scratched. Removing needle head, sucking exogenous material into needle tube, lightly holding the upper surface of cotyledon by hand, and driving the liquid into cotyledon along wound.
Further, the needle in step (3) only breaks the lower epidermis and cannot pierce the cotyledons; the application time of the exogenous material can be selected before or after grafting, and the application time can be specifically designed according to an experimenter.
Further, the management of the grafting healing period in the step (4) may adopt the following process:
covering with transparent plastic film 1 day before grafting to prevent scion death caused by water evaporation; 2-4 days after grafting, ventilation is carried out for 0.5-4 hours every day, 4-8 days after grafting, ventilation time is gradually increased every day, the ventilation time is not too long, the scion is just not withered to be optimal, and after grafting for 8 days, the plastic film is removed; the temperature in the grafting healing period is slightly higher than the normal culture temperature, 26-28 ℃ in the daytime and 18-20 ℃ at night. When sunlight is strong during grafting healing, a sunshade net needs to be covered, so that weak light (80 mu mol.m) is ensured 10 days before grafting seedling -2 ·s -1 ) Status of
The invention relates to a precise application method of exogenous substances, which is applied to grafting.
The precise application method of the exogenous material is applied to the development of the grafted seedling strengthening reagent.
The main action parts for effectively distinguishing the exogenous substances are scions, stocks or both.
The invention relates to a method for improving the quality of grafted seedlings, which is characterized in that auxin is applied to scions by adopting the exogenous material precise application method.
The invention relates to a method for improving the quality of grafted seedlings, which is characterized in that auxin is applied to a stock by adopting the exogenous material precise application method.
The invention relates to a method for improving the quality of grafted seedlings, which is characterized in that auxin is simultaneously applied to scions and stocks by adopting the exogenous material precise application method.
In summary, the invention has the advantages and positive effects that: the patent develops a method for accurately applying exogenous substances for strong seedling cultivation of grafted seedlings, which can effectively and accurately apply exogenous substances to scions and stocks respectively, further judge whether the action part of the exogenous substances is a scion or a stock or both, and is favorable for developing the strong seedling reagent of grafted seedlings such as grafting healing agent special for the scions.
Drawings
FIG. 1A is a graph showing the effect of exogenous substances on melon cotyledons before accurate application
FIG. 1B is a graph showing the effect of the exogenous material applied to cotyledons of melons (water stains represent the entry of exogenous material into cotyledons)
FIG. 2 is a statistical analysis of the quality of grafted seedlings with auxin applied to scion, stock and scion stock simultaneously
Note that: strong seedling index= (scion stem thickness/plant height) ×root surface area
Detailed Description
Example 1
A precise application method of exogenous substances for strong seedling cultivation of grafted seedlings comprises the following steps:
(1) Selecting scions and stocks: the melon variety 'old iron sheet' is selected as scion. A good pumpkin stock variety 'Qing you stock No. 1' with good affinity and strong stress resistance is selected as the stock.
(2) Scion and stock seedling:
sterilizing melon scions and pumpkin stock seeds with 5% sodium hypochlorite for 10min, cleaning with tap water, and soaking for 6-8h at room temperature; accelerating germination at a constant temperature of 30 ℃ until radicle is exposed to white, and broadcasting the radicle in a flat disc; grafting the 1 st true leaves when the leaves are slightly exposed.
(3) Preparation before grafting:
the blade used for grafting and the grafting clamp are sterilized by 75% medical alcohol in advance and naturally dried for standby; distilled water is used for preparing 0.1mM auxin as an exogenous substance, and the auxin is preserved at 4 ℃ in dark for standby.
(4) Grafting scion and stock:
adopting monocotyledonous sticking, obliquely cutting the scion at an angle of 30 degrees at a position 1cm below the basal part of the cotyledon, and making the section smooth; the stock is obliquely cut at an angle of 30 degrees at the basal part of cotyledons, a cotyledon and all growing points are cut off, and the section is smooth; and tightly attaching the stock to the cutting surface of the scion, and fixing the grafting clip.
(5) Accurate application of exogenous substances:
after grafting, 1ml of medical syringe was taken.
A: accurate application of scions: the tip of the needle was slid slightly on the back of the cotyledon of the scion to form a lower epidermal wound of about 2-3mm in length, but the cotyledon could not be scratched. Removing needle head, sucking exogenous material into needle tube, lightly holding the upper surface of cotyledon by hand, and driving the liquid into cotyledon along wound. About 0.5ml per scion was injected.
B: accurate application of the stock: the needle tip was slid slightly on the back of the cotyledon of the stock to form a lower epidermal wound of about 2-3mm in length, but the cotyledon could not be scratched. Removing needle head, sucking exogenous material into needle tube, lightly holding the upper surface of cotyledon by hand, and driving the liquid into cotyledon along wound. About 0.5ml of each stock was injected.
C: accurate application of scions and stocks: the needle tip was slid slightly on the back of the cotyledon of the scion and the stock to form a lower epidermal wound of about 2-3mm in length, but the cotyledon could not be scratched. Removing needle head, sucking exogenous material into needle tube, lightly holding the upper surface of cotyledon by hand, and driving the liquid into cotyledon along wound. About 0.5ml of each scion and stock are injected.
(6) Management of grafting healing period:
covering with transparent plastic film 1 day before grafting to prevent scion death caused by water evaporation; 2-4 days after grafting, ventilation is carried out for 0.5-4 hours every day, 4-8 days after grafting, ventilation time is gradually increased every day, the ventilation time is not too long, the scion is just not withered to be optimal, and after grafting for 8 days, the plastic film is removed; the temperature in the grafting healing period is slightly higher than the normal culture temperature, 26-28 ℃ in the daytime and 18-20 ℃ at night. When sunlight is strong during grafting healing, a sunshade net needs to be covered, so that weak light (80 mu mol.m) is ensured 10 days before grafting seedling -2 ·s -1 ) Status of the device.
(7) Experimental results:
the results show that: the quality of grafted seedlings can be obviously improved by simultaneously applying auxin to the stock, the scion and the scion, but the seedling strengthening effect of the scion application is obviously better than that of the stock and the scion and the stock.
The foregoing description of the preferred embodiments of the invention is not intended to be limiting, but rather is intended to cover all modifications, equivalents, and alternatives falling within the spirit and principles of the invention.
Claims (10)
1. A method for accurately applying exogenous substances for strong seedling cultivation of grafted seedlings comprises the following steps of (1) raising seedlings of scions and stocks; (2) grafting scions with the stock; (3) precise application of exogenous substances; and (4) managing the grafting healing period.
2. The method of claim 1, wherein the precise application of the exogenous material in step (3) can be performed by the following steps: taking 1ml medical injector, and lightly sliding the needle point on the back of cotyledon of scion or stock to form lower epidermis wound with length of about 2-3mm, but not scratching cotyledon; removing needle head, sucking exogenous material into needle tube, lightly holding the upper surface of cotyledon by hand, and driving the liquid into cotyledon along wound.
3. The method of claim 1, wherein in step (3) the needle breaks only the lower epidermis and cannot pierce the cotyledons; the application time of the exogenous material can be selected before grafting or after grafting.
4. The method of claim 1, wherein the precise application of the scion in step (3) is as follows: the needle point is used for sliding slightly on the back of the cotyledon of the scion to form a lower epidermis wound with the length of about 2-3mm, but the cotyledon cannot be scratched; removing the needle head, sucking exogenous substances into the needle tube, lightly propping against the upper surface of the cotyledon by hand, and driving the liquid in the needle tube into the cotyledon along the wound; about 0.5ml per scion was injected.
5. The method of claim 1, wherein the precise application of the rootstock in step (3): the needle point is used for sliding slightly on the back of the cotyledon of the stock to form a lower epidermis wound with the length of about 2-3mm, but the cotyledon cannot be scratched; removing the needle head, sucking exogenous substances into the needle tube, lightly propping against the upper surface of the cotyledon by hand, and driving the liquid in the needle tube into the cotyledon along the wound; about 0.5ml of each stock was injected.
6. The method of claim 1, wherein in step (3) the precise application of scions to the rootstock: the needle point is used for sliding slightly on the back surfaces of cotyledons of scions and stocks to form a lower epidermis wound with the length of about 2-3mm, but the cotyledons cannot be scratched; removing needle head, sucking exogenous material into needle tube, lightly holding the upper surface of cotyledon by hand, and driving the liquid into cotyledon along wound. About 0.5ml of each scion and stock are injected.
7. Use of the method according to claim 1 in grafting.
8. The method as claimed in claim 1, which is applied to the development of a reagent for strengthening grafted seedlings.
9. The use of the method of claim 1, wherein the primary site of action for effective foreign material discrimination is scion, rootstock, or both.
10. A method for improving the quality of grafted seedlings, applying auxin to scions by the method of claim 4 or applying auxin to stocks by the method of claim 5 or applying auxin to scions and stocks simultaneously by the method of claim 6.
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| Application Number | Priority Date | Filing Date | Title |
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| CN202211502015.9A CN116076252A (en) | 2022-11-28 | 2022-11-28 | Precise application method of exogenous substances for strong seedling cultivation of grafted seedlings |
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| CN202211502015.9A CN116076252A (en) | 2022-11-28 | 2022-11-28 | Precise application method of exogenous substances for strong seedling cultivation of grafted seedlings |
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Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1593091A (en) * | 2004-07-12 | 2005-03-16 | 黑龙江省牡丹江林业科学研究所 | Pine subgenus evergreen arbor needle oil grafting technology |
| CN102337291A (en) * | 2011-07-27 | 2012-02-01 | 湖南农业大学 | Method for gene transient expression of citrus |
| CN104303851A (en) * | 2014-09-28 | 2015-01-28 | 定远县金胜农业开发有限公司 | Tomato grafting method capable of improving survival rate |
| CN108048601A (en) * | 2017-12-20 | 2018-05-18 | 江苏省农业科学院 | A kind of capsicum Cucumber Mosaic Virus Seedling Inoculation method and its application |
| CN114223426A (en) * | 2021-12-27 | 2022-03-25 | 中国热带农业科学院热带生物技术研究所 | Monocotyledon leaf liquid injection method |
-
2022
- 2022-11-28 CN CN202211502015.9A patent/CN116076252A/en active Pending
Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1593091A (en) * | 2004-07-12 | 2005-03-16 | 黑龙江省牡丹江林业科学研究所 | Pine subgenus evergreen arbor needle oil grafting technology |
| CN102337291A (en) * | 2011-07-27 | 2012-02-01 | 湖南农业大学 | Method for gene transient expression of citrus |
| CN104303851A (en) * | 2014-09-28 | 2015-01-28 | 定远县金胜农业开发有限公司 | Tomato grafting method capable of improving survival rate |
| CN108048601A (en) * | 2017-12-20 | 2018-05-18 | 江苏省农业科学院 | A kind of capsicum Cucumber Mosaic Virus Seedling Inoculation method and its application |
| CN114223426A (en) * | 2021-12-27 | 2022-03-25 | 中国热带农业科学院热带生物技术研究所 | Monocotyledon leaf liquid injection method |
Non-Patent Citations (1)
| Title |
|---|
| 傅达奇著: "植物中病毒诱导基因沉默技术操作指导", 30 November 2016, 中国农业大学出版社, pages: 52 - 53 * |
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