CN103931477A - Novel acacia melanoxylon tissue culture seedling rooting method - Google Patents
Novel acacia melanoxylon tissue culture seedling rooting method Download PDFInfo
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- CN103931477A CN103931477A CN201410175953.1A CN201410175953A CN103931477A CN 103931477 A CN103931477 A CN 103931477A CN 201410175953 A CN201410175953 A CN 201410175953A CN 103931477 A CN103931477 A CN 103931477A
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- seedling
- black wooden
- transplanting
- tissue cultured
- root
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Abstract
The invention discloses a novel acacia melanoxylon tissue culture seedling rooting method. The method includes the steps that small holes with the depths of 1.0-1.5 cm are uniformly formed in a watered transplanting substrate through inoculation tweezers in a pricking mode firstly; an acacia melanoxylon rootless stem bud cultured through a strong seedling is taken out of a tissue culture bottle, culture media of a base portion are washed clean with tap water, then the acacia melanoxylon rootless stem bud is cut off from the base portion with an inoculation cutter, and leaves are cut off until 3-5 leatherleaves are left around an apical bud through the inoculation cutter; the acacia melanoxylon rootless stem bud is put in a rooting accelerant to be immersed for a certain period of time, then a small seedling is clamped through the tweezers to be inserted into the transplanting substrate, the transplanting substrate is pressed to be compact, and the exposed apical bud and the leaves exposed are 1.5-2.0 cm away from the upper portion of the transplanting substrate; ultimately, a culture box is placed in a domestication chamber where lighting intensity and lighting time are set. According to the method, root quality is greatly improved, loss caused by pollution is reduced, the survival rate of transplanted seedlings is improved, the seedling cycle is shortened due to the fact that rooting, domestication and transplanting are combined, and production cost is greatly saved.
Description
Technical field
The present invention relates to plant tissue culture fast breeding technique field.Be specifically related to a kind of black wooden Tissue Cultured Acacia seedling rooting new method.
Background technology
Plant tissue culture fast breeding technique is developed so far day by day ripe, yet also still there are problems, particularly black wooden Tissue Cultured Acacia fast breeding technique was studied in recent years the stage in stagnation always, fail to find one effectively can raising group train seedling quality, can shorten growing-seedling period again, the method reducing costs; And taking root of black wooden Tissue Cultured Acacia seedling also needs to improve root system quality, shorten rootage duration, improve transplanting survival rate.
Summary of the invention
The object of the present invention is to provide a kind of reducing costs, shorten growing-seedling period, improve the black wooden Tissue Cultured Acacia seedling rooting new method of transplanting survival rate.
For achieving the above object, the invention provides a kind of black wooden Tissue Cultured Acacia seedling rooting new method, it is characterized in that, comprise the steps,
First on the transplanting medium that has watered certain volume water, with inoculation tweezers, stab into one by one the approximately dark aperture of 1.0 ~ 1.5cm equably;
Black wooden yearning between lovers unrooted stem eye through strong seedling culture is taken out in tissue culture bottle, with running water, base portion medium is cleaned up, then black wooden yearning between lovers stem eye is scaled off from base portion with vaccinating lancet, then remain 3 ~ 5 leatherleafs near blade being switched to terminal bud with vaccinating lancet; Put into after root-inducing promoter soaks certain hour and clamp seedling insertion transplanting medium compacting with tweezers, expose terminal bud and blade 1.5 ~ 2.0cm above; Finally cultivating box, be placed in the domestication chamber cultivation that sets intensity of illumination and light irradiation time.
Described transplanting medium is turfy soil, sawdust and perlitic mixture, and its volume ratio is 6:3:1.
In the transplanting medium of described certain volume water, moisture content is 40 volume % ~ 50 volume %.
Described root-inducing promoter is ABT
1, concentration is 200 ~ 300mg/L, soaks 10 ~ 20min.
Described intensity of illumination is 90 ~ 135 μ molm
-2s
-1, light irradiation time is 12 ~ 16 h.
The object of the invention is by black wooden yearning between lovers choiceness group training seedling is carried out to the open research of taking root, environment conditioning technology (take root matrix type and moisture content, the kind of root-inducing promoter, concentration and processing time, different luminous environments (intensity of illumination and periodicity of illumination)) be applied to taking root of black wooden Tissue Cultured Acacia seedling, summary draws and is applicable to the open optimal environmental condition of taking root of black wooden yearning between lovers choiceness group training seedling, and the rooting rate of black wooden Tissue Cultured Acacia seedling can reach more than 90%, do not need to carry out hardening, can directly be transplanted in nutritious bag, survival rate reaches more than 95%, apply the transplanting survival rate that this invention has not only improved black wooden Tissue Cultured Acacia seedling, and shortened growing-seedling period, reduced cost, for black wooden Tissue Cultured Acacia seedling rooting provides a new technology.
Black wooden Tissue Cultured Acacia seedling of the present invention derives from the black wooden yearning between lovers choiceness seedling of geographical (Xiamen) bio tech ltd, and experiment place is geographical (Xiamen) domestication chamber, bio tech ltd, temperature (
) ℃, humidity 80%-90%.
The present invention consists of following test:
A, different transplanting medium are on the open impact of taking root of black wooden Tissue Cultured Acacia seedling: consider 4 kinds of matrix: 1. turfy soil; 2. turfy soil+sawdust (2:1); 3. turfy soil+perlite (3:1); 4. turfy soil+sawdust+perlite (6:3:1).Black wooden yearning between lovers unrooted stem eye need not be processed by any root-inducing promoter, and cultivating intensity of illumination is 90 μ molm
-2s
-1, cultivate the photoperiod and be 12h/12h(light/dark);
The different water cut of B, transplanting medium is on the open impact of taking root of black wooden Tissue Cultured Acacia seedling: pour into the score of matrix volume Wei 1:14,1:12, the running water of 1:10,1:8, until water fully mixes with matrix, in each box, evenly fetch earth, do 3 repetitions, the culture dish (m that the soil of taking-up is placed on to preprepared and has weighed
0) in, after taking, weigh the gross mass (m of culture dish and soil sample
1), put into baking oven and arrive constant weight with 80 ℃, treat that culture dish temperature drops to room temperature, the soil sample after weighing is dried and the gross mass (m of culture dish
2) (being accurate to 0.01g);
；
The matrix moisture content calculating under each processing by above formula is respectively 30.6%, 40.7%, 50.6%, 60.5%;
The matrix of using in this test is turfy soil+sawdust+perlite (6:3:1), and black wooden yearning between lovers unrooted stem eye need not be processed by any root-inducing promoter, and cultivating intensity of illumination is 90 μ molm
-2s
-1, cultivate the photoperiod and be 12h/12h(light/dark);
The kind of C, root-inducing promoter, concentration and processing time are on the open impact of taking root of black wooden Tissue Cultured Acacia seedling: consider IAA, NAA, IBA, ABT
1four kinds of root-inducing promoters (in Table 1), every kind all adopts 100mgL
-1, 200mgL
-1, 300mgL
-1, 400mgL
-1four levels, each concentration is soaked without rhizome clump base portion 5,10,20,40min.The matrix of using in this test is turfy soil+sawdust+perlite (6:3:1), and cultivating intensity of illumination is 90 μ molm
-2s
-1, cultivate the photoperiod and be 12h/12h(light/dark);
D, different illumination intensity are on the open impact of taking root of black wooden Tissue Cultured Acacia seedling: consider four kinds of intensity of illumination (22.5 μ molm
-2s
-1, 45 μ molm
-2s
-1, 90 μ molm
-2s
-1, 135 μ molm
-2s
-1), matrix of using in this test is turfy soil+sawdust+perlite (6:3:1), black wooden yearning between lovers unrooted stem eye need not be processed by any root-inducing promoter, cultivates the photoperiod and be 12h/12h(light/dark);
E, different photoperiod are on the open impact of taking root of group training seedling: consider 8h/16h(light/dark), 10h/14h(light/dark), 14h/10h(light/dark), 16h/8h(light/dark) four kinds of periodicity of illuminations and 12h/12h(light/secretly) compare.The matrix of using in this test is turfy soil+sawdust+perlite (6:3:1), and black wooden yearning between lovers unrooted stem eye need not be processed by any root-inducing promoter, and cultivating intensity of illumination is 90 μ molm
-2s
-1.
Remarkable advantage of the present invention is: in traditional black wooden yearning between lovers bottle of comparing, take root, black wooden Tissue Cultured Acacia seedling is carried out to open taking root and can greatly improve root system quality, and can reduce the loss bringing because polluting, improved transplanting survival rate.In addition, the present invention also has a significance, and the present invention combines taking root, tame and transplanting of black wooden Tissue Cultured Acacia seedling, has shortened growing-seedling period, has greatly saved production cost.
Method of operating: first stab into one by one the approximately dark aperture of 1.0 ~ 1.5cm with inoculation tweezers equably on the transplanting medium that has watered water.Black wooden yearning between lovers unrooted stem eye through strong seedling culture is taken out in tissue culture bottle, with running water, base portion medium is cleaned up, then black wooden yearning between lovers stem eye is scaled off from base portion with vaccinating lancet, near blade being switched to terminal bud with vaccinating lancet again, remain 3 ~ 5 leatherleafs, then with tweezers, clamp seedling and insert in matrix and matrix compacting, expose terminal bud and blade 1.5 ~ 2.0cm above.Cuttage is complete should be noted following some: one, with 0.1% carbendazim or tpn, spray stromal surface, first week is every spray in a day once, and each week sprays once afterwards.Two, to spray blade face mode, water once every day.Three, for adding forced ventilation, cultivate after one week, prop 1/2 after lid being propped to 1/4, two week with tweezers, after surrounding, can uncap completely.Four, to regulate indoor humidity to be at least 90% after uncapping, increase in addition blade face irrigation times, avoid blade dehydration.
Best Applying Condition:
(1) being applicable to the open best transplanting medium of taking root of black wooden yearning between lovers choiceness group training seedling is turfy soil+sawdust+perlite (6:3:1), and optimum substrate moisture content is 40% ~ 50%;
(2) being applicable to the open best root-inducing promoter of taking root of black wooden yearning between lovers choiceness group training seedling is ABT
1, optium concentration is 200 ~ 300mg/L, optimum pretreatment time is 10 ~ 20min;
(3) being applicable to the open optimum illumination intensity of taking root of black wooden yearning between lovers choiceness group training seedling is 90 ~ 135 μ molm
-2s
-1, optimum illumination duration is 12 ~ 16 h.
Accompanying drawing explanation
Fig. 1 is the seedling figure cultivating after 7d;
Fig. 2 cultivates the seedling figure after 30d.
embodiment
Describe embodiments of the invention below in detail, the example of described embodiment is shown in the drawings, and wherein same or similar label represents same or similar element or has the element of identical or similar functions from start to finish.Below by the embodiment being described with reference to the drawings, be exemplary, be intended to for explaining the present invention, and can not be interpreted as limitation of the present invention.Unreceipted concrete technology or condition person in embodiment, according to the described technology of the document in this area or condition or carry out according to product description.The unreceipted person of production firm of agents useful for same or instrument, being can be by the conventional products of commercial acquisition.
Black wooden Tissue Cultured Acacia seedling derives from the black wooden yearning between lovers choiceness seedling of geographical (Xiamen) bio tech ltd, and experiment place is geographical (Xiamen) domestication chamber, bio tech ltd, temperature (
) ℃, humidity 80%-90%.
embodiment 1:black wooden Tissue Cultured Acacia seedling rooting
Method of operating: first stab into one by one the approximately dark aperture of 1.0 ~ 1.5cm with inoculation tweezers equably having watered on the transplanting medium of water (turfy soil+sawdust+perlitic mixture, volume ratio is 6:3:1).Black wooden yearning between lovers unrooted stem eye through strong seedling culture is taken out in tissue culture bottle, with running water, base portion medium is cleaned up, then black wooden yearning between lovers stem eye is scaled off from base portion with vaccinating lancet, near blade being switched to terminal bud with vaccinating lancet again, remain 3 ~ 5 leatherleafs, then with tweezers, clamping seedling cuttage enters in matrix and matrix compacting, expose terminal bud and blade 1.5 ~ 2.0cm above, finally cultivating box, be placed in 90 ~ 135 μ molm
-2s
-1, cultivate the domestication chamber that light irradiation time is 12 ~ 16h.
Cuttage is complete should be noted following some: one, with 0.1% carbendazim or tpn, spray stromal surface, first week is every spray in a day once, and each week sprays once afterwards.Two, to spray blade face mode, water once every day.Three, for adding forced ventilation, cultivate after one week, with tweezers, lid is propped to 1/4(and see Fig. 1), after two weeks, prop 1/2, the (see figure 2) of can uncapping completely after surrounding.Four, to regulate indoor humidity to be at least 90% after uncapping, increase in addition blade face irrigation times, avoid blade dehydration.
Embodiment 2: the selection of different experimental conditions
A, different transplanting medium are on the open impact of taking root of black wooden Tissue Cultured Acacia seedling: consider 4 kinds of matrix: 1. turfy soil; 2. turfy soil+sawdust (2:1); 3. turfy soil+perlite (3:1); 4. turfy soil+sawdust+perlite (6:3:1).Black wooden yearning between lovers unrooted stem eye need not be processed by any root-inducing promoter, and cultivating intensity of illumination is 90 μ molm
-2s
-1, cultivate the photoperiod and be 12h/12h(light/dark).
Draw by experiment: being applicable to the open best transplanting medium of taking root of black wooden yearning between lovers choiceness group training seedling is turfy soil+sawdust+perlite (6:3:1),
The different water cut of B, transplanting medium is on the open impact of taking root of black wooden Tissue Cultured Acacia seedling: pour into the score of matrix volume Wei 1:14,1:12, the running water of 1:10,1:8, until water fully mixes with matrix, in each box, evenly fetch earth, do 3 repetitions, the culture dish (m that the soil of taking-up is placed on to preprepared and has weighed
0) in, after taking, weigh the gross mass (m of culture dish and soil sample
1), put into baking oven and arrive constant weight with 80 ℃, treat that culture dish temperature drops to room temperature, the soil sample after weighing is dried and the gross mass (m of culture dish
2) (being accurate to 0.01g).
The matrix moisture content calculating under each processing by above formula is respectively 30.6%, 40.7%, 50.6%, 60.5%.
The matrix of using in this test is turfy soil+sawdust+perlite (6:3:1), and black wooden yearning between lovers unrooted stem eye need not be processed by any root-inducing promoter, and cultivating intensity of illumination is 90 μ molm
-2s
-1, cultivate the photoperiod and be 12h/12h(light/dark).
Result draws: when matrix is turfy soil+sawdust+perlite (6:3:1), optimum substrate moisture content is 40% ~ 50%.
The kind of C, root-inducing promoter, concentration and processing time are on the open impact of taking root of black wooden Tissue Cultured Acacia seedling: consider IAA, NAA, IBA, ABT
1four kinds of root-inducing promoters, every kind all adopts 100mgL
-1, 200mgL
-1, 300mgL
-1, 400mgL
-1four levels, each concentration is soaked without rhizome clump base portion 5,10,20,40min.The matrix of using in this test is turfy soil+sawdust+perlite (6:3:1), and cultivating intensity of illumination is 90 μ molm
-2s
-1, cultivate the photoperiod and be 12h/12h(light/dark).
Table 1
| ? | English full name | Chinese name |
| IAA | indole propionic acid | Heteroauxin |
| NAA | a-Naphthalene acetic acid | Methyl α-naphthyl acetate |
| IBA | indole butyric acid | Indolebutyric acid |
| ABT 1 | ? | ABT1 root-inducing powder |
Result draws: being applicable to the open best root-inducing promoter of taking root of black wooden yearning between lovers choiceness group training seedling is ABT
1, optium concentration is 200 ~ 300mg/L, optimum pretreatment time is 10 ~ 20min.
D, different illumination intensity are on the open impact of taking root of black wooden Tissue Cultured Acacia seedling: consider four kinds of intensity of illumination (22.5 μ molm
-2s
-1, 45 μ molm
-2s
-1, 90 μ molm
-2s
-1, 135 μ molm
-2s
-1), matrix of using in this test is turfy soil+sawdust+perlite (6:3:1), black wooden yearning between lovers unrooted stem eye need not be processed by any root-inducing promoter, cultivates the photoperiod and be 12h/12h(light/dark).
Result is learnt: being applicable to the open optimum illumination intensity of taking root of black wooden yearning between lovers choiceness group training seedling is 90 ~ 135 μ molm
-2s
-1.
E, different photoperiod are on the open impact of taking root of group training seedling: consider 8h/16h(light/dark), 10h/14h(light/dark), 14h/10h(light/dark), 16h/8h(light/dark) four kinds of periodicity of illuminations and 12h/12h(light/secretly) compare.The matrix of using in this test is turfy soil+sawdust+perlite (6:3:1), and black wooden yearning between lovers unrooted stem eye need not be processed by any root-inducing promoter, and cultivating intensity of illumination is 90 μ molm
-2s
-1.
Result is learnt, when optimum illumination intensity is 90 ~ 135 μ molm
-2s
-1time, optimum illumination duration is 12 ~ 16 h.
Although illustrated and described embodiments of the invention above, be understandable that, above-described embodiment is exemplary, can not be interpreted as limitation of the present invention, those of ordinary skill in the art can change above-described embodiment within the scope of the invention in the situation that not departing from principle of the present invention and aim, modification, replacement and modification.
Claims (5)
1. a black wooden Tissue Cultured Acacia seedling rooting new method, is characterized in that, comprise the steps,
First on the transplanting medium that has watered certain volume water, with inoculation tweezers, stab into one by one the approximately dark aperture of 1.0 ~ 1.5cm equably;
Black wooden yearning between lovers unrooted stem eye through strong seedling culture is taken out in tissue culture bottle, with running water, base portion medium is cleaned up, then black wooden yearning between lovers stem eye is scaled off from base portion with vaccinating lancet, then remain 3 ~ 5 leatherleafs near blade being switched to terminal bud with vaccinating lancet; Put into after root-inducing promoter soaks certain hour and clamp seedling insertion transplanting medium compacting with tweezers, expose terminal bud and blade 1.5 ~ 2.0cm above; Finally cultivating box, be placed in the domestication chamber cultivation that sets intensity of illumination and light irradiation time.
2. black wooden Tissue Cultured Acacia seedling rooting new method described in claim 1, is characterized in that, described transplanting medium is turfy soil, sawdust and perlitic mixture, and its volume ratio is 6:3:1.
3. black wooden Tissue Cultured Acacia seedling rooting new method described in claim 1, is characterized in that, in the transplanting medium of described certain volume water, moisture content is 40 volume % ~ 50 volume %.
4. black wooden Tissue Cultured Acacia seedling rooting new method described in claim 1, is characterized in that, described root-inducing promoter is ABT
1, concentration is 200 ~ 300mg/L, soaks 10 ~ 20min.
5. black wooden Tissue Cultured Acacia seedling rooting new method described in claim 1, is characterized in that, described intensity of illumination is 90 ~ 135 μ molm
-2s
-1, light irradiation time is 12 ~ 16 h.
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|---|---|---|---|
| CN201410175953.1A CN103931477A (en) | 2014-04-29 | 2014-04-29 | Novel acacia melanoxylon tissue culture seedling rooting method |
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|---|---|---|---|
| CN201410175953.1A CN103931477A (en) | 2014-04-29 | 2014-04-29 | Novel acacia melanoxylon tissue culture seedling rooting method |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108419552A (en) * | 2018-06-07 | 2018-08-21 | 福建省泉州罗溪国有林场 | A kind of black wooden yearning between lovers root turion cuttage and seedling culture method |
| CN110583407A (en) * | 2019-10-18 | 2019-12-20 | 青岛市农业科学研究院 | Radish seed-replacing and reserving method |
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2014
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN108419552A (en) * | 2018-06-07 | 2018-08-21 | 福建省泉州罗溪国有林场 | A kind of black wooden yearning between lovers root turion cuttage and seedling culture method |
| CN110583407A (en) * | 2019-10-18 | 2019-12-20 | 青岛市农业科学研究院 | Radish seed-replacing and reserving method |
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