CN112956348B - Muskmelon grafting compatibility stock screening method - Google Patents
Muskmelon grafting compatibility stock screening method Download PDFInfo
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- 244000064895 Cucumis melo subsp melo Species 0.000 title 1
- 235000015510 Cucumis melo subsp melo Nutrition 0.000 claims abstract description 52
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- ZCYVEMRRCGMTRW-UHFFFAOYSA-N 7553-56-2 Chemical compound [I] ZCYVEMRRCGMTRW-UHFFFAOYSA-N 0.000 claims abstract description 35
- 229910052740 iodine Inorganic materials 0.000 claims abstract description 35
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- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 claims abstract description 8
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G2/00—Vegetative propagation
- A01G2/30—Grafting
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G22/00—Cultivation of specific crops or plants not otherwise provided for
- A01G22/05—Fruit crops, e.g. strawberries, tomatoes or cucumbers
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N1/00—Sampling; Preparing specimens for investigation
- G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
- G01N1/30—Staining; Impregnating ; Fixation; Dehydration; Multistep processes for preparing samples of tissue, cell or nucleic acid material and the like for analysis
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
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- G01N21/84—Systems specially adapted for particular applications
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- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N1/00—Sampling; Preparing specimens for investigation
- G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
- G01N1/30—Staining; Impregnating ; Fixation; Dehydration; Multistep processes for preparing samples of tissue, cell or nucleic acid material and the like for analysis
- G01N2001/302—Stain compositions
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/84—Systems specially adapted for particular applications
- G01N2021/8466—Investigation of vegetal material, e.g. leaves, plants, fruits
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Abstract
The incompatibility of melon grafting is a problem often faced in growth, and the development of an efficient affinity rootstock early screening technology is urgently needed. The invention belongs to the technical field of stock screening, and discloses a melon grafting compatibility stock screening method, which comprises the steps of respectively disinfecting and cleaning melon stock seeds and melon scion seeds, soaking the seeds and accelerating germination; grafting by adopting a monocotyledon grafting manner; when the grafting heals for 7 days, quickly putting a stem section of 2mm of the healed part of the grafting scion into 2ml of 75% medical alcohol, fixing and decoloring for 24 hours, and storing at room temperature; taking out the decolorized stem sample, washing in tap water for 1min, dyeing with pre-prepared iodine solution for 1min, and washing in tap water for 1 min; taking out and placing on filter paper; and (4) observing by using a stereoscopic microscope, calculating the iodine staining area percentage of starch of the stem section of the scion healing part, screening the compatible rootstocks based on the calculation result, and if the iodine staining area is less than 10%, determining the grafted compatible rootstocks. The method can complete the screening of the compatible stock material 7 days after the melon grafting, and has the advantages of high accuracy, time and labor saving and low cost.
Description
Technical Field
The invention belongs to the technical field of rootstock screening, and particularly relates to a method for screening grafting compatibility rootstocks of melons.
Background
At present, the melon is one of ten fruits in the world, is an important horticultural crop in China, and plays a great role in promoting the income of farmers and meeting the increasing melon and fruit requirements of people. However, in recent years, the problem of continuous cropping obstacle of soil is prominent due to the perennial fixed-place cultivation of farmers, the occurrence of soil-borne diseases and insect pests such as blight and the like is becoming more serious, the normal growth of melons is seriously affected, and the development of the melon industry is hindered. Grafting and root replacement can effectively prevent the problem.
But the grafting proportion of the melons in China is still low at present, mainly because of the lack of high-quality compatible melon stock resources. Poor grafting compatibility is a problem often faced in melon grafting cultivation, and the occurrence of official events in production due to grafting incompatibility often occurs. In the past, compatible stock materials are screened by scientific research units, seedling companies or farmers, melon seedlings grafted on different stocks are planted in fields, the later growth condition, the fruit yield, the fruit quality and the like of the grafted seedlings are detected, and the compatibility is judged. The whole process is long in time consumption, consumes a large amount of manpower and material resources and is high in cost.
Through the above analysis, the problems and defects of the prior art are as follows: the existing melon grafting affinity screening rootstock process is long in time consumption, consumes a large amount of manpower and material resources and is high in cost.
The difficulty in solving the above problems and defects is: the method is based on the fact that the scion and the rootstock cannot be effectively communicated with each other due to grafting incompatibility of the rootstock, and carbohydrate is blocked to be transported at the scion grafting healing part and is converted into starch, so that dyeing can be carried out according to iodine dyeing, and the grafting compatibility of the melon rootstock can be judged. The difficulty in solving the above problems is moderate.
The significance of solving the problems and the defects is as follows: by the method, the grafting compatibility of the stock and the melon can be judged 7 days after grafting, and the method is rapid, high in accuracy, time-saving, labor-saving and low in cost. The method has important significance for the seed selection and grafting cultivation of the melon stocks, promotes the large-area application of the grafted melons in production, and reduces the production loss caused by grafting incompatibility in production.
Disclosure of Invention
Aiming at the problems in the prior art, the invention provides a muskmelon grafting compatibility stock screening method.
The invention is realized in such a way that the screening method of the melon grafting compatibility stock comprises the following steps:
respectively carrying out stock seedling culture and scion seedling culture, treating the stock and the scion when the 1 st true leaf of the stock is completely flattened and the 1 st true leaf of the scion is slightly exposed, grafting by adopting a monocotyledon grafting mode, and carrying out grafting affinity screening by calculating the iodine staining area of a grafting stem.
Further, the method for screening the melon grafting compatibility rootstock comprises the following steps:
step one, respectively disinfecting stock seeds and scion seeds of the melons; respectively cleaning, and soaking seeds for 6-8 h at room temperature; accelerating germination of the melon stock seeds after seed soaking at the constant temperature of 30 ℃ for 24-48 h, and sowing the seeds in 50-hole trays when radicles are exposed to white, wherein 1 hole is formed;
step two, accelerating germination of the soaked melon scion seeds at the constant temperature of 30 ℃ for 24 hours, and broadcasting the seeds in a flat disc when radicles are exposed to white; grafting by adopting a monocotyledon grafting mode when the 1 st true leaf of the stock is completely flattened and the 1 st true leaf of the scion is slightly exposed;
step three, managing the grafting healing period; and after 7d of grafting healing, taking a stem section of 2mm of the healing part of the grafting scion, namely a grafting healing interface, quickly putting the stem section into 2ml of 75% medical alcohol, fixing and decoloring for 24h, and storing at room temperature;
taking out a decolorized stem sample, washing the stem sample in tap water for 1min, dyeing the stem sample for 1min by using a prepared iodine solution, and washing the stem sample in the tap water for 1 min; taking out and placing on filter paper for 2s-3 s;
and fifthly, observing by a stereoscopic microscope, calculating the percentage of the iodine-stained area of the starch of the stem section of the scion healing part, and screening the compatible rootstock based on the calculation result.
Further, in step one, the sterilizing comprises: sterilizing with 1 ‰ potassium permanganate for 5 min.
Further, in the second step, before grafting by adopting a monocotyledon grafting mode when the 1 st true leaf of the stock is completely flattened and the 1 st true leaf of the scion is slightly exposed, the following steps are required:
disinfecting the blade and the grafting clip with 75% medical alcohol, and naturally drying; and the matrix is watered thoroughly 1 day before grafting.
Further, in the second step, the grafting by adopting the monocotyledon grafting manner comprises:
treating the rootstock: removing all true leaves, and cutting off a cotyledon with a blade at the base of the cotyledon by inclining 45 degrees downwards, wherein the cut surface is about 0.5 cm;
scion treatment: the grafting blade is obliquely cut at an angle of about 45 degrees upwards at a position 0.5cm-1.0cm away from the base part of the cotyledon, and the section is about 0.5 cm;
the stock is closely attached to the section of the scion, and a grafting clip is clipped.
Further, in step three, the performing the management of the graft healing period comprises:
temperature management: 28-25 ℃ in the daytime and 22-18 ℃ at night;
humidity management: the humidity of the grafted product is kept above 95% in the 3d period;
ventilating 4d-6d after grafting, wherein the ventilation time is that the cotyledon of the scion is not wilted;
7d, opening the film, and entering normal management;
illumination management: after grafting, the light intensity is kept at 80 mu mol/m in the dark at 1d and in the low light after 1d2S; after 1 week, normal management was performed.
Further, the iodine dye solution preparation comprises the following steps:
dissolving 8.8g of KI in 30mL of distilled water, then adding 2.2g of crystal iodine for dissolving to obtain a mother solution, and placing the mother solution in a brown reagent bottle for storage at normal temperature;
1mL of the mother liquor is dissolved in 30mL of tap water for dilution to obtain the iodine dye solution.
Further, the calculation formula of the iodine-staining area percentage of the starch of the stem segment of the scion healing part is as follows:
further, the affinity rootstock screening based on the calculation result comprises: if the iodine staining area percentage is less than 10%, the rootstock is the compatible rootstock.
By combining all the technical schemes, the invention has the advantages and positive effects that: the invention provides a muskmelon grafting compatibility stock screening method, which can complete screening of compatibility stock materials (table 1) 7 days after grafting, shows obvious difference in iodine staining percentage of scion healing stem sections, and is high in accuracy, time-saving, labor-saving and low in cost. The traditional method needs at least 28d, the plant height and dry weight of the scions do not show significant difference after grafting at 7d, and the compatibility cannot be judged until 28d shows significant difference (table 2 and table 3).
Results and required time of three screening methods for different stocks in the prior art and experimental comparison of the invention show that:
TABLE 1 screening results and time required for this method on different stocks
Note: the letters in the same column indicate that the difference reaches a significant level after multiple comparisons of Duncan (P <0.05)
TABLE 2 results and time required for screening different stocks by conventional screening methods (plant height data)
Note: the letters in the same column indicate that the difference reaches a significant level after multiple comparisons of Duncan (P <0.05)
TABLE 3 results and time required for conventional screening methods for different rootstocks (scion dry weight data)
Note: the letters in the same column indicate that the difference reaches a significant level after multiple comparisons of Duncan (P <0.05)
Drawings
In order to more clearly illustrate the technical solutions of the embodiments of the present application, the drawings needed to be used in the embodiments of the present application will be briefly described below, and it is obvious that the drawings described below are only some embodiments of the present application, and it is obvious for those skilled in the art that other drawings can be obtained from the drawings without creative efforts.
FIG. 1 is a schematic diagram of an efficient and effective screening method for melon grafting compatibility stocks provided by the embodiment of the invention.
FIG. 2 is a flow chart of the efficient and effective screening method for melon grafting compatibility rootstocks provided by the embodiment of the invention.
FIG. 3 is a schematic diagram of the phenotype of the melon grafted and healed with four stocks for 7d and the iodine staining condition of the starch of the scion stem segment provided by the embodiment of the invention.
In the figure: FIG. 3(a) Halloween anvil (compatible rootstock); FIG. 3(b) Green Excellent stock No. one (compatible stock); figure 3(c) jingxin rootstock No. (incompatible rootstock); FIG. 3(d) Ansheng cucurbita pepo (incompatible rootstock).
FIG. 4 is a schematic diagram of the phenotype and the iodine-staining condition of the starch of the scion stem segment after the melon is grafted with four stocks for 28 d.
In the figure: FIG. 4(a) Halloween anvil (compatible rootstock); FIG. 4(b) Green Excellent stock No. one (compatible stock); figure 4(c) jingxin rootstock No. (incompatible rootstock); FIG. 4(d) Ansheng cucurbita pepo (incompatible rootstock).
Detailed Description
In order to make the objects, technical solutions and advantages of the present invention more apparent, the present invention is further described in detail with reference to the following embodiments. It should be understood that the specific embodiments described herein are merely illustrative of the invention and are not intended to limit the invention.
Aiming at the problems in the prior art, the invention provides a method for screening melon grafting compatibility rootstocks, and the method is described in detail below with reference to the accompanying drawings.
As shown in fig. 1, the method for screening melon grafting compatibility rootstocks provided by the embodiment of the present invention comprises:
respectively carrying out stock seedling culture and scion seedling culture, treating the stock and the scion when the 1 st true leaf of the stock is completely flattened and the 1 st true leaf of the scion is slightly exposed, grafting by adopting a monocotyledon grafting mode, and carrying out grafting affinity screening by calculating the iodine staining area of a grafting stem.
As shown in fig. 2, the method for screening melon grafting compatibility rootstocks provided by the embodiment of the invention comprises the following steps:
s101, sterilizing rootstock seeds of the melons and scion seeds of the melons for 5min by using 1 per thousand potassium permanganate respectively; respectively cleaning, and soaking seeds for 6-8 h at room temperature; accelerating germination of the melon stock seeds after seed soaking at the constant temperature of 30 ℃ for 24-48 h, and sowing the seeds in 50-hole trays when radicles are exposed to white, wherein 1 hole is formed;
s102, accelerating germination of the soaked melon scion seeds at a constant temperature of 30 ℃ for 24 hours, and broadcasting the seeds in a flat disc when radicles are exposed to white; grafting by adopting a monocotyledon grafting mode when the 1 st true leaf of the stock is completely flattened and the 1 st true leaf of the scion is slightly exposed;
s103, managing the grafting healing period; when the grafting heals for 7 days, taking a stem section of 2mm of the healed part of the grafting scion, namely a grafting heals interface, quickly putting the stem section into 2ml of 75% medical alcohol, fixing and decoloring for 24 hours, and storing at room temperature;
s104, taking out a decolorized stem sample, washing the stem sample in tap water for 1min, dyeing the stem sample for 1min by using a prepared iodine solution, and washing the stem sample in the tap water for 1 min; taking out and placing on filter paper for 2s-3 s;
and S105, observing by using a stereoscopic microscope, calculating the percentage of the iodine-stained area of the starch of the stem section of the scion healing part, and screening the compatible rootstock based on the calculation result.
In step S102, before grafting by adopting the monocotyledon grafting method when the 1 st true leaf of the rootstock is completely flattened and the 1 st true leaf of the scion is slightly exposed, the steps of:
disinfecting the blade and the grafting clip with 75% medical alcohol, and naturally drying; and the matrix is watered thoroughly 1d before grafting.
In step S102, the grafting by the monocotyledon grafting method according to the embodiment of the present invention includes:
treating the rootstock: removing all true leaves, and cutting off a cotyledon with a blade at the base of the cotyledon by inclining 45 degrees downwards, wherein the cut surface is about 0.5 cm;
scion treatment: the grafting blade is obliquely cut at an angle of about 45 degrees upwards at a position 0.5cm-1.0cm away from the base part of the cotyledon, and the section is about 0.5 cm;
the stock is closely attached to the section of the scion, and a grafting clip is clipped.
In step S103, the management for performing the graft healing period provided by the embodiment of the present invention includes:
temperature management: 28-25 ℃ in the daytime and 22-18 ℃ at night;
humidity management: the humidity of the grafted product is kept above 95% in the 3d period;
ventilating 4d-6d after grafting, wherein the ventilation time is that the cotyledon of the scion is not wilted;
7d, opening the film, and entering normal management;
illumination management: after grafting, the light intensity is kept at 80 mu mol/m in the dark at 1d and in the low light after 1d2S; after 1 week, normal management was performed.
The iodine dye solution provided by the embodiment of the invention comprises the following components:
dissolving 8.8g of KI in 30mL of distilled water, then adding 2.2g of crystal iodine for dissolving to obtain a mother solution, and placing the mother solution in a brown reagent bottle for storage at normal temperature;
1mL of the mother liquor is dissolved in 30mL of tap water for dilution to obtain the iodine dye solution.
The calculation formula of the iodine staining area percentage of the starch of the stem section of the scion healing part provided by the embodiment of the invention is as follows:
the affinity rootstock screening based on the calculation result provided by the embodiment of the invention comprises the following steps: if the iodine staining area percentage is less than 10%, the rootstock is the compatible rootstock.
The technical solution of the present invention is further illustrated by the following specific examples.
Example (b):
efficient screening technology for melon stock grafting compatibility stock
(1) Melon and stock variety: in the embodiment of the scheme, four melon stocks including 'Qingyou stock I', 'Ansheng gourd', 'Jingxin stock I' and 'Qingsheng stock' are selected as melon stocks, and 'Akkouqi' of melons is selected as melon scion
(2) Stock seedling culture: sterilizing melon stock seeds by 1 per thousand potassium permanganate for 5min, cleaning, and soaking seeds at room temperature for 6-8 h; then accelerating germination at constant temperature of 30 ℃ for 24-48 h, sowing the radicles in a 50-hole tray when the radicles are exposed to white, 1 hole for 1 grain, and grafting the 1 st true leaves when the leaves are completely spread.
(2) Scion seedling culture: sterilizing melon scion seeds for 5min by 1 per mill potassium permanganate, cleaning, and soaking seeds for 6-8 h at room temperature; then accelerating germination at constant temperature of 30 ℃ for 24h, broadcasting the radicles in a flat dish when the radicles are exposed to white, and grafting when the 1 st true leaves are slightly exposed.
(3) And (3) grafting period: stock: 1, completely flattening true leaves, and grafting: 1, exposing true leaves to micro dew; and the matrix is completely watered thoroughly one day before grafting.
(4) Grafting operation: adopting monocotyledon grafting and rootstock treatment: removing all true leaves by hand, and cutting off a cotyledon with a blade at 45-degree slant at the base of the cotyledon, wherein the cut surface is about 0.5 cm; scion treatment: the grafting blade is obliquely cut at an angle of about 45 degrees upwards at a position 0.5cm-1.0cm away from the base part of the cotyledon, and the section is about 0.5 cm; and finally, tightly attaching the stock to the scion section and clamping the grafting clamp.
(6) Managing the grafting healing period:
controlling the temperature at 28-25 ℃ in the daytime and 22-18 ℃ at night, and managing the humidity: the humidity of the grafted stem is kept above 95% in the 3d period. May be covered with a plastic film. Ventilating gradually at 4-6 days after grafting, wherein the ventilation time is preferably no wilting of the cotyledon of the scion. And 7d, opening the film, and entering normal management. Illumination management: after grafting, the light intensity is kept at 80 mu mol/m in the dark at 1d and in the low light after 1d2S. After 1 week, normal management was performed.
(7) And (3) screening grafting affinity:
grafting stem section sampling: after grafting healing for 7 days, a stem section (above a grafting healing interface) of 2mm at the healing part of the grafting scion is taken and quickly placed into 2ml of 75% medical alcohol, fixed and decolored for 24h, and stored at room temperature.
Preparing an iodine dye solution: 8.8g of KI is dissolved in 30mL of distilled water, then 2.2g of crystal iodine is added for dissolution, and the brown reagent bottle is stored at normal temperature. 1mL of the mother liquor was dissolved in 30mL of tap water before each use.
Iodine staining of stem sections: taking out the decolorized stem sample, washing with tap water for 1min, dyeing with iodine solution for 1min, and washing with tap water for 1min (removing background color); thereafter, the sheet was taken out and placed on a filter paper, and excess water was sucked off (about 2s to 3 s).
Photographing and observing: observing by a stereomicroscope, and calculating the percentage of iodine-stained starch of stem sections of the scion healing parts by imagej software:
TABLE 1 screening results and time required for this method on different stocks
Note: different letters in the same column indicate that the difference reaches a significant level (P <0.05) after multiple comparisons of Duncan, Table 2 shows that the traditional screening method has screened results and required time (plant height data) for different stocks
Note: the letters in the same column indicate that the difference reaches a significant level after multiple comparisons of Duncan (P <0.05)
TABLE 3 results and time required for conventional screening methods for different rootstocks (scion dry weight data)
Note: the letters in the same column indicate that the difference reaches a significant level after multiple comparisons of Duncan (P <0.05)
In the invention, fig. 3 is a schematic diagram of phenotypes and scion stem starch iodine staining conditions when melons are grafted with four stocks for 7d, which are provided by the embodiment of the invention.
In the figure: FIG. 3(a) Halloween anvil (compatible rootstock); FIG. 3(b) Green Excellent stock No. one (compatible stock); figure 3(c) jingxin rootstock No. (incompatible rootstock); FIG. 3(d) Ansheng cucurbita pepo (incompatible rootstock).
FIG. 4 is a schematic diagram of the phenotype and the iodine-staining condition of the starch of the scion stem segment after the melon is grafted with four stocks for 28 d.
In the figure: FIG. 4(a) Halloween anvil (compatible rootstock); FIG. 4(b) Green Excellent stock No. one (compatible stock); figure 4(c) jingxin rootstock No. (incompatible rootstock); FIG. 4(d) Ansheng cucurbita pepo (incompatible rootstock).
The above description is only for the purpose of illustrating the present invention and the appended claims are not to be construed as limiting the scope of the invention, which is intended to cover all modifications, equivalents and improvements that are within the spirit and scope of the invention as defined by the appended claims.
Claims (3)
1. A muskmelon grafting compatibility stock screening method comprises the following steps: respectively carrying out stock seedling raising and scion seedling raising, treating the stock and the scion when the 1 st main leaf of the stock is completely flattened and the 1 st main leaf of the scion is slightly exposed, and grafting by adopting a monocotyledon grafting mode, and the melon grafting compatibility stock screening method is characterized by further comprising the following steps of:
performing grafting affinity screening by calculating the iodine staining area of stem sections of the healing parts of the grafting scions; the method for screening grafting affinity specifically comprises the following steps:
managing the grafting healing period; when the grafting heals for 7 days, taking a stem section of 2mm of the healed part of the grafting scion, namely 2mm above a grafting healed interface, quickly putting the stem section into 2ml of 75% medical alcohol, fixing and decoloring for 24 hours, and storing at room temperature;
taking out the decolorized stem sample, washing in tap water for 1min, dyeing with pre-prepared iodine solution for 1min, and washing in tap water for 1 min; taking out and placing on filter paper for 2s-3 s;
observing by a stereoscopic microscope, calculating the percentage of iodine-stained area of starch of the stem section of the scion healing part, and screening the compatible rootstock based on the calculation result;
the screening method of the melon grafting compatibility rootstock further comprises the following steps:
sterilizing the stock seeds and the scion seeds of the melons by 1 per thousand potassium permanganate for 5min respectively; respectively cleaning, and soaking seeds for 6-8 h at room temperature; accelerating germination of the melon stock seeds after seed soaking at the constant temperature of 30 ℃ for 24-48 h, and sowing the seeds in 50-hole trays when radicles are exposed to white, wherein 1 hole is formed;
accelerating germination of the melon scion seeds after seed soaking at the constant temperature of 30 ℃ for 24h, and broadcasting the seeds in a flat disc when radicles are exposed to white; grafting by adopting a monocotyledon grafting mode when the 1 st true leaf of the stock is completely flattened and the 1 st true leaf of the scion is slightly exposed;
and when the 1 st true leaf of the stock is completely flattened and the 1 st true leaf of the scion is slightly exposed, grafting is carried out by adopting a monocotyledon grafting mode, wherein the grafting is carried out by adopting the following steps:
disinfecting the blade and the grafting clip with 75% medical alcohol, and naturally drying; and watering the matrix thoroughly 1 day before grafting;
the grafting by adopting the monocotyledon grafting manner comprises the following steps:
treating the rootstock: removing all true leaves, and cutting off a cotyledon with a blade at the cotyledon base part obliquely at 45 degrees downwards to obtain a cotyledon with a section of 0.5 cm;
scion treatment: the grafting blade is obliquely cut upwards at an angle of about 45 degrees at a position 0.5cm-1.0cm away from the base part of the cotyledon, and the section is 0.5 cm;
closely attaching the stock to the section of the scion, and clamping a grafting clamp;
the management for performing the graft healing period comprises:
temperature management: 28-25 ℃ in the daytime and 22-18 ℃ at night;
humidity management: the humidity of the grafted product is kept above 95% in the 3d period;
ventilating 4d-6d after grafting, wherein the ventilation time is that the cotyledon of the scion is not wilted;
7d, opening the film, and entering normal management;
the calculation formula of the iodine staining area percentage of the starch of the stem section of the scion healing part is as follows:
the affinity rootstock screening based on the calculation result comprises the following steps: if the iodine staining area percentage is less than 10%, the rootstock is the compatible rootstock.
2. The method for screening melon engrafting compatible rootstocks according to claim 1, wherein said managing for grafting healing period further comprises: illumination management: after grafting, the light intensity is kept at 80 mu mol/m in the dark at 1d and in the low light after 1d2S; after 1 week, normal management was performed.
3. The method for screening melon grafting compatibility rootstock according to claim 1, wherein the iodine staining solution preparation comprises:
dissolving 8.8g of KI in 30mL of distilled water, then adding 2.2g of crystal iodine for dissolving to obtain a mother solution, and placing the mother solution in a brown reagent bottle for storage at normal temperature;
1mL of mother liquor is dissolved in 30mL of tap water for dilution to obtain iodine dye solution.
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| US4937971A (en) * | 1987-04-08 | 1990-07-03 | Groupement Champenois D'exploitation Viticole | Process of grafted cuttings of stock and scion from in vitro |
| JP2001340021A (en) * | 2000-06-01 | 2001-12-11 | Nippon Del Monte Corp | Vegetable's multi-kind laterally multi-branched seedling |
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| JP2001340021A (en) * | 2000-06-01 | 2001-12-11 | Nippon Del Monte Corp | Vegetable's multi-kind laterally multi-branched seedling |
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