CN116034746A

CN116034746A - Cutting propagation method of female plant of Liguo

Info

Publication number: CN116034746A
Application number: CN202211587102.9A
Authority: CN
Inventors: 向明剑; 谢松林; 吴松; 毛富平
Original assignee: Sichuan Lide Seedling Technology Co ltd
Current assignee: Sichuan Lide Seedling Technology Co ltd
Priority date: 2022-12-10
Filing date: 2022-12-10
Publication date: 2023-05-02

Abstract

The invention belongs to the technical field of Litsea japonica propagation, and discloses a cutting propagation method of Litsea japonica female plants, which comprises the following steps: collecting a tip section of a female plant twig of the Litsea japonica as a cutting slip; refrigerating for 4 weeks at a low temperature of 1-5 ℃, treating the cutting slips, sterilizing, removing leaves below 3cm below the lower part of the tip section, and retaining terminal buds and surrounding leaves; filling the sterilized cutting matrix into a plug, compacting and watering thoroughly for standby, soaking the lower part of the cutting slip with 100mg/L NAA for 0.5h, watering 30ml of 100mg/L NAA solution for the cutting slip and the nearby matrix every 5d after cutting slip cutting, and watering 2 times; after hormone irrigation, the mixture is placed in a greenhouse for cultivation. The method selects the young branch tip section of the female plant of the beautiful fruit tree as the cutting slip, and the cutting rooting rate can reach more than 95 percent after low-temperature treatment and hormone treatment, thereby effectively solving the problems of low seedling raising amount and low forming speed in the actual gardening production of the beautiful fruit tree.

Description

Cutting propagation method of female plant of Liguo

Technical Field

The invention belongs to the technical field of beautiful fruit tree propagation, and relates to a cutting propagation method of an female beautiful fruit tree strain.

Background

Litsea (Gaultheria mucronata), the Ericaceae family white-bead tree belongs to evergreen shrubs, and is grown in high-altitude areas above the tree lines of the volcanic areas in the south of Argentina and in the south of Chilean, compact and dense. It is cool and moist and resistant to low temperature. The plant height can reach 1.5 meters, the length of the leaves is about 1cm, and the plant has the characteristics of innovation and dark green luster; white flowers, single plants, and 5-6 months of flowering period; dense berries are planted at the tips of branches, the fruits are ripe in autumn, and the fruits can be harvested to the early spring, and have white, pink, red and the like.

The beautiful fruit tree is novel and peculiar and has great ornamental value, the main ornamental value is that fruits are covered on the plant branches at every fruit period, and the beautiful fruit tree can be used as an excellent fruit-observing potted plant and branch-cutting material in gardening production, so that the variety of the ornamental fruit plant is enriched.

At present, the Liguo tree is generally subjected to seed propagation and tissue culture propagation, and the Liguo tree is low in seed sowing emergence rate, irregular in emergence, difficult to maintain and the like due to the fact that the Liguo tree seeds are small, and is limited by factors such as seasons and the like, so that large-scale seedling cultivation is difficult to carry out in a short time. In addition, the beautiful fruit tree is a hermaphrodite plant, seeds are needed to be obtained through artificial pollination during sowing and propagation, and the seed setting rate is low. The tissue culture seedlings have the defect of low plant forming speed. Therefore, a cutting propagation method of the Litsea coreana is required to be developed, and the problems of low seed propagation emergence rate and low tissue culture propagation forming speed in actual production are effectively solved, so that reasonable development and utilization of the Litsea coreana are promoted.

Disclosure of Invention

The invention provides a cutting propagation method of a female plant of Litsea coreana, aiming at the problems of low seed propagation emergence rate and low tissue culture propagation forming speed in the propagation of Litsea coreana, wherein the method selects the young branch tip section of the female plant of Litsea coreana as a cutting slip, and the rooting rate of the cutting slip can reach more than 95% after low-temperature treatment and hormone treatment, so that the problems of low seedling raising amount and low forming speed in the actual gardening production of Litsea coreana are effectively solved, and reasonable development and utilization of Litsea coreana resources can be accelerated.

In order to achieve the above purpose, the present invention adopts the following technical scheme:

the invention provides a cutting propagation method of a female plant of Liguo, which comprises the following steps:

a. collecting a tip section of a healthy and strong female plant twig of the beautiful fruit tree without plant diseases and insect pests as a cutting slip, wherein the length of the tip section is 8cm; b. refrigerating at 1-5 deg.c for 4 weeks to treat cutting, sterilizing, eliminating leaf below 3cm below the lower part of the tip section and maintaining the terminal bud and surrounding leaf;

c. filling the sterilized cutting matrix into a plug, compacting and watering thoroughly for standby, soaking the lower part of the cutting slip with 100mg/L NAA for 0.5h, watering 30ml of 100mg/L NAA solution for the cutting slip and the nearby matrix every 5d after cutting slip cutting, and watering 2 times;

d. after hormone irrigation, placing the mixture into a greenhouse for culturing for 40 days, wherein the greenhouse conditions are as follows: the temperature is 22-28 ℃, the illumination intensity is 2000-5000lx, and the spraying is carried out every 30min in the daytime by using a timing spraying facility.

Further, the method further comprises the following steps before the step a: 2 months before picking, spraying 500 times 2% of benzyl amino purine every 10 days for 1 time, and 3 times in total, so as to promote the light branches of the Litsea coreana.

Further, the method further comprises the following steps after the step a: and (5) uniformly spraying Shi Li female flowers of fruit trees with 200mg/L ethephon 3 days before cutting, and removing the female flowers.

Further, the cutting matrix is vermiculite and peat with a volume ratio of 3:2.

Compared with the prior art, the invention has the beneficial effects that:

according to the invention, researches on branch treatment, branch material selection, matrix proportion and hormone treatment and later management are carried out, and through the comparative tests on the branch material selection, matrix proportion and hormone treatment, the young branch tip section of the female plant of the Liguo tree is finally selected as a cutting slip, and after low-temperature treatment and hormone treatment, the rooting rate can reach more than 95%, so that the problems of low seedling raising amount and low forming speed in actual gardening production of the Liguo tree are effectively solved, and reasonable development and utilization of Liguo tree resources can be accelerated.

Drawings

FIG. 1 shows the rooting of test number 63 according to the invention.

Fig. 2 shows the normal growth of the seedlings of Litsea coreana after the application of the pot.

Detailed Description

The following examples are illustrative of the present invention and are not intended to limit the scope of the invention. The technical means used in the examples are conventional means well known to those skilled in the art unless otherwise indicated. The test methods in the following examples are conventional methods unless otherwise specified.

Example 1

1.1 selecting test materials

The Liguo wood is a hermaphrodite plant, a large number of female plants are produced in gardening, and a small number of male plants are used for pollination, so that the female plants are selected as female parent for scion. And collecting the tip section, the middle section and the basal section of the young branches of the healthy and healthy female plants of the beautiful fruit tree without plant diseases and insect pests as cutting slips. The application amount of the phosphorus fertilizer and the potassium fertilizer is gradually reduced 1 month before the cutting is collected, the application amount of the nitrogen fertilizer is maintained, and watering is reduced 7-10 days before the cutting is collected, so that the pot soil is slightly dried, the growth of branches is prolonged and slowed down, and the branches are more robust.

The growth characteristics of the Liguo woods are good upright property and poor branching property, the quantity of the cutting shoots obtained by a single female parent is limited, and the rapid propagation of Yu Li woods is unfavorable. 2 months before picking, spraying 500 times 2% of benzyl amino purine every 10 days for 3 times, and promoting the light branches of the Liguo woods.

When in cutting, 75% alcohol is used for disinfecting both hands and the branch scissors, then the cutting is carried out, and the both hands and the cutting blades are sprayed with 75% alcohol every half an hour for disinfecting. The Liguo wood is native to high altitude areas, and the collected cutting slips may need low temperature treatment to improve rooting rate during propagation in plain.

And using a special plug for cutting the forest tree with 7 cm deep holes of 72 holes as a cutting container, uniformly spraying and sterilizing the upper surface and the lower surface of the plug by using 1000 times of potassium permanganate solution, and airing for later use.

1.2 cutting treatment

And at the position of uniformly spraying Shi Li female flowers of fruit tree by using 200mg/L ethephon 3 days before cutting, removing female flowers of the female parent of the fruit tree for cutting, thereby reducing the workload of manual flower removal. The slightly-staged, middle-staged and basal sections of the female branches of the Litsea coreana are processed at low temperature (refrigerated for 4 weeks at 1-5 ℃) before cutting, and the hormone processing mode is as follows: 100mg/L NAA soaked the spike for 0.5h at 1cm below. After the cutting, 30ml of 100mg/L NAA solution was poured into the cutting and the nearby substrate every 5 days, and the total amount of pouring was 2 times.

Removing leaves below 3cm below the lower part of the 8cm tip section treated at low temperature, retaining terminal buds and surrounding leaves, soaking the lower part for 1-2cm deep with 800 times of 50% carbendazim solution, sterilizing for 30min, and washing with distilled water for use.

1.3 rooting conditions of different matrix proportions

The cutting matrix is vermiculite and peat, and the matrix is sterilized: spraying 800-1000 times potassium permanganate solution on the surface of the substrate, and covering the film for 3-5 days. And filling the matrix into the hole tray 1 day before cutting, compacting, and fully watering and watering for standby.

And (3) treating the slightly-cut sections of the female plant twigs of the Liguo tree according to the step (1.2), adopting different cutting medium ratios, and counting rooting conditions after 40 days. The results are shown in Table 1.

TABLE 1 Effect of different cutting substrates on rooting of cutting slips of Litsea

As shown in Table 1, when the volume ratio of vermiculite to peat in the matrix is 3:2, the rooting rate is 97.5%, which is obviously superior to the rooting rate of other ratios as cutting matrix.

1.4 rooting conditions of different cutting positions

Vermiculite and peat with a matrix volume ratio of 3:2 are used as matrixes, the slightly-staged, the middle-staged and the basal stage of the female plant twig of the beautiful fruit tree are treated according to the step 1.2, and the non-low-temperature treatment is used as a control. And counting rooting condition after 40 days. The results are shown in Table 2.

TABLE 2 influence of different cutting positions on rooting of cutting of Litsea

As is clear from Table 2, the rooting rate was different depending on the selected portion of the insert and whether the insert was subjected to low temperature treatment (1-5 ℃ C. For 4 weeks), and the rooting rate was 97.5% in the shoot segment (test No. 63) after the low temperature treatment, which had the highest rooting rate.

1.5 rooting Condition treated with different hormones

The tip section treated at low temperature is used as cutting, vermiculite and peat with the volume ratio of matrix of 3:2 are used as matrixes, and after different hormone treatments, the beautiful fruit tree is inserted into the matrixes prepared 1 day in advance with the depth of 2-3 cm. In the test, 20 cuttings are treated by each hormone, and the treatment is repeated for 3 times, and the rooting conditions are counted after 40 days, wherein the rooting conditions comprise rooting rate, average root number and average root length. The hormone removing treatment modes in the test treatment are different, and the source and the part of cutting, the cutting matrix, the post management and the like are consistent. The results are shown in Table 3.

TABLE 3 Effect of different hormone concentrations on rooting of Litsea wood cuttings

As shown in Table 3, the rooting rate was 97.5% as high as the rooting rate of the cuttings treated with 100mg/L NAA before and after the cutting.

Rooting for test number 63 is shown in FIG. 1.

1.7 post-management

After hormone treatment and cuttage are completed, the hormone is put into a greenhouse for daily management at the temperature of 22-28 ℃ and the illumination intensity of 2000-5000lx, and a timing spraying facility is used for spraying for 10s every 30min in the daytime. After about 15 days of cutting, root tips begin to appear, and the root systems of substantially all the beautiful fruit trees can cluster the matrix after 40 days.

And then transplanting the female plants into a pot to obtain female plant Liguo wood potted plants, and carrying out daily maintenance, wherein the survival rate can reach 100%. The seedlings of the Liguo tree which normally grow after being put on the pot are shown in figure 2.

The above-mentioned embodiments are merely preferred embodiments of the present invention, which are not intended to limit the scope of the present invention, and other embodiments can be easily made by those skilled in the art through substitution or modification according to the technical disclosure in the present specification, so that all changes and modifications made in the principle of the present invention shall be included in the scope of the present invention.

Claims

1. The cutting propagation method of the female plant of the Litsea coreana is characterized by comprising the following steps of:

a. collecting a tip section of a healthy and strong female plant twig of the beautiful fruit tree without plant diseases and insect pests as a cutting slip, wherein the length of the tip section is 8cm;

b. refrigerating for 4 weeks at a low temperature of 1-5 ℃, treating the cutting slips, sterilizing, removing leaves below 3cm below the lower part of the tip section, and retaining terminal buds and surrounding leaves;

2. The cutting propagation method of the female plant of beautiful fruit tree according to claim 1, which is characterized by further comprising the following steps before the step a: 2 months before picking, spraying 500 times 2% of benzyl amino purine every 10 days for 1 time, and 3 times in total, so as to promote the light branches of the Litsea coreana.

3. The cutting propagation method of the female plant of beautiful fruit tree according to claim 1, which is characterized by further comprising the following steps after the step a: and (5) uniformly spraying Shi Li female flowers of fruit trees with 200mg/L ethephon 3 days before cutting, and removing the female flowers.

4. The cutting propagation method of the female plant of beautiful fruit tree according to claim 1, wherein the cutting substrate is vermiculite and peat with a volume ratio of 3:2.