KR20120021618A - Propagation method of juniperus chinensis var. sargentii using cutting - Google Patents

Abstract

PURPOSE: An increasing method of juniperus chinensis using cuttings is provided to process scions collected from the juniperus chinensis with a rooting promoter. CONSTITUTION: An increasing method of juniperus chinensis using cuttings comprises the following steps: processing scions collected from the juniperus chinensis with a rooting promoter by dipping the bases of the scions in the rooting promoter for 3-7 minutes; and planting the scions processed with the rooting promoter in culture soil at April or May. The length of the scions is 7-10cm.

Description

Propagation method of perennial tree using cuttings {Propagation method of Juniperus chinensis var. sargentii using cutting}

The present invention provides a method for processing as specifically cuttings taken from the eye of the eye than juniper juniper (Juniperus chinensis var sargentii.) Growth method using the cuttings (cutting) by a rooting promoter; It relates to a fragrance tree propagation method using the cuttings and the fragrance tree growth method using the cuttings, characterized in that it comprises the step of inserting the cuttings treated with the rooting promoter in the culture soil.

Snow juniper (Juniperus chinensis var. Sargentii) is also referred to as lying juniper, devastation trees nunsang tree evergreen coniferous shrubs gymnosperms taxales cupressaceae. It grows in the cracks of high mountains, and its main stem stands at an angle or extends to the ground. It is similar to juniper, but grows to the side and its branches are winding. Leaves are sharp needles when young, but do not poke like cedar, but when they grow old, they have only scaly leaves. There are two white lines wider than the veins, and the back is blueish green. Scale leaves alternate with small branches, rhombic, rounded.

Flowers bloom in May and are male and female. The male flower is egg-shaped, 2mm long, and the female flower is ball-shaped and hangs on the end of the branch. Fruits are coniferous and ripen in October of the following year. It is blue, ball-shaped, and contains 1 ~ 3 eggs in the shape of eggs.

As the main stem stands at an angle or extends to the ground, it is mainly used for ornamental and landscaping. It is distributed in Korea (Jeju, Gyeongbuk, Gangwon, Pyeongbuk, Pyeongnam, Hambuk), Japan, Taiwan, and Sakhalin Island.

There are many ways to breed plants, and among them, cutting of one species, a method of asexual breeding, is the fastest and most accurate way to maintain and propagate parasite traits. Many attempts have been made for the propagation of.

Among the studies on cutting propagation, most attempts have been made to increase the rooting rate of cuttings using rooting promoters, and cutting times, cutting methods, topsoil, cutting parameters, and harvesting sites have also been reported. Berhe and Negash, 1998; Negash, 2002; Song et al., 2006; Kim et al., 2006).

Depending on the plant, the right time for cutting is different, and the timing of cutting is closely related to rooting. According to Hartmann et al. (1990), evergreen conifers are hardwood in spring and summer. It is reported that better results than cutting.

In addition, rooting agents may be treated on the cutting surface of the cuttings in order to improve roots during cutting in cutting of plants using cuttings. Attention was 86% at IBA 200 mgL ^-1 (11% untreated: Kim and Nam, 1985), larch at 80% at IBA 1000 mgL ^-1 (20-52% untreated: Chung and Lee, 1994), Snowy cedar is untreated, but 93% or more in IAA 1000mgL- ¹ and NAA 500mgL- ¹ (Song et al. , 2006), and birch tree is 86% in IBA 100mgL- ¹ (untreated 75.2% : Jung et al ., 2009) reported the best cutting results, respectively.

However, the proliferation of the fragrance tree has not been studied yet. The inventors of the present invention use the cutting of the fragrance tree through the identification of the harvest time, the cutting time, and the type and concentration of the rooting agent to be treated in the cutting of the fragrance tree. To provide a method of propagation of.

It is an object of the present invention to provide a method for propagating fragrance trees using cuttings, and more particularly, to provide a method for growing fragrance trees using cuttings.

Another object of the present invention is to provide a fragrance tree obtained by the fragrance tree growth method using the above-mentioned cutting.

The present invention comprises the steps of treating the spatula collected from the fragrant wood with a rooting promoter; It is possible to provide a fragrance tree propagation method using the cuttings, comprising the step of inserting the cuttings treated with the rooting promoter in cultured soil.

The present invention can provide a fragrance tree obtained by the fragrance tree growth method using the above-mentioned cutting.

The present invention is to provide a method for propagating fragrance trees by using the cutting of the asexual breeding method to provide a method for propagating fragrant trees facing the endangered world.

On the other hand, in the growth method of the fragrance tree using the cutting of the present invention, the callus formation rate is higher and rooting is promoted compared to the untreated (control) without the rooting agent in the cutting time, the cutting time and the cutting of the fragrance tree. By identifying the type and concentration of the promoter can provide a method for growing perennial tree using the best cutting.

In particular, the method of propagating the fragrant tree using the cutting of the present invention can maintain the genetically identical traits of the fragrant tree because it uses the uncultivated breeding method. It can lead to solving the problem of sexual growth. In addition, the production time of seedlings can be shortened and mass production at a low cost can greatly contribute to the tree-related industry.

FIG. 1 is a photograph showing a clump of cedar tree collected from the cuttings.
Figure 2 is a graph showing the rooting rate (Rooting rate) according to the cutting time (cutting time) of the fragrance tree.
Figure 3 is a graph showing the rooting rate (Rooting rate) of the fragrance bark cutting according to the rooting agent treatment of the fragrance bark insertion when cutting the fragrance.
Figure 4 is a photograph showing the rooting of the fragrance tree after 3 months of cutting after IBA 1000mgL ^-1 treatment on the fragrance tree.

The present invention shows a method for growing perennial tree using cuttings.

The present invention comprises the steps of treating the spatula collected from the fragrant wood with a rooting promoter; Represents a method for growing fragrance wood using cuttings comprising inserting the above-mentioned rooting agent-treated insert into cultured soil.

In the above, the cutting of the incense can be carried out in April-May.

In the above, the cutting of the incense can be carried out in May.

The cuttings collected from the fragrant wood can be treated with a rooting promoter of indolebutyl acid (IBA) 500-1000 mgL ^-1 .

The cuttings collected from the fragrant wood can be treated with a rooting promoter of IBA 1000mgL- ¹ .

The above-mentioned cuts from the fragrance wood can be treated with a rooting accelerator of indole acetic acid (IAA) 500-1000 mgL ^-1 .

The cuts collected from the fragrant wood can be treated with an IAA 500mgL- ¹ rooting promoter.

The above-mentioned cuts from the fragrant wood can be treated with a rooting accelerator of 200-500 mgL ^-1 of naphthalene acetic acid (NAA).

The cuts collected from the fragrant wood can be treated with a rooting promoter of NAA 500mgL- ¹ .

The cuts collected from the fragrance trees can be treated with IBA 500-1000 mgL ^-1 , IAA 500-1000 mgL ^-1 or NAA 200-500 mgL ^-1 rooting promoter.

The cuts collected from the fragrance trees can be treated with IBA 1000mgL- ¹ , IAA 500mgL- ¹ or NAA 500mgL- ¹ rooting promoter.

In this case, the rooting agent-treated insert can be inserted into the culture soil in April-May.

In the above, the rooting agent-treated insert can be inserted into the culture soil in May.

When inserting the rooting agent-treated cuttings in the culture soil in the above it is good to perform within the shortest time after collecting the cuttings from the fragrant wood. That is, after cutting the cuttings from the fragrant tree, it is recommended to treat the cuttings with a rooting promoter and immediately insert the cuttings.

In the above time when inserting the rooting agent-treated cuttings into the culture soil, it is preferable to treat the cuttings taken from the perennial tree in April and May with rooting agents and inserting them in April and May.

The cuttings collected from the fragrance trees are 7-10 cm in length and can be used for shoots of fragrance trees of 1 to 3 years.

The cuttings collected from the fragrance tree are 8 cm long and can be used to shoot a 2 year old fragrance tree.

Treatment of the cuttings collected from the fragrant wood with rooting promoter can be immersed in the rooting agent for 2.5 to 3.5cm in the rooting of the cuttings collected from the fragrant wood for 3-7 minutes.

Treatment of the cuttings collected from the fragrant wood with rooting promoter can be immersed in the rooting agent for 5 minutes to 3 cm of the base of the cuttings collected from the fragrant wood.

The cultured soil may be a mixed soil in which sand: pitmos: vermiculite is mixed in the same volume ratio.

The cultured soil may be a mixed soil in which sand: pitmos: vermiculite is mixed in a volume ratio of 1: 1: 1 :.

The bark tree growth method using the cutting of the present invention was carried out under various conditions, in order to achieve the object of the present invention, it is preferable to provide a bark tree growth method using the cutting by the above-mentioned conditions.

The present invention includes a bark bark cut obtained by the bark bark propagation method using the above-mentioned bark.

The present invention includes the fragrance tree obtained by the fragrance tree growth method using the above-mentioned cutting.

Hereinafter, the content of the present invention will be described in detail through experimental examples and examples. However, these are for the purpose of illustrating the present invention in more detail, and the scope of the present invention is not limited thereto.

Experimental Example

1. Materials and Methods

The test materials were collected on May 10, 2008 and August 7, 2008, respectively, from the persimmon tree population growing at 1,520m above the mountainside of Seoraksan (see Fig. 1). In particular, the test fixture was placed so that one clump could be repeated for each of three clumps that could be prepared with a sufficient amount of inserts.

The cuts taken on May 10, 2008 were cut in the rainforest greenhouse on May 10, 2008. The cuts taken on August 7, 2008, were cut in the rainforest greenhouse on August 7, 2008. Thus, the inserts were inserted at different times.

After cutting, the shading net was installed to prevent the temperature rise in the non-raining greenhouse. During the cutting period (9 months), the temperature and humidity environment in the greenhouse were maintained at 27.5 ± 9.1 ℃ and 56.3 ± 15.4%, respectively.

In the above, the cutting was 8 cm long so that 2 years of raw material was included, and the base was immersed in the rooting agent for 3 minutes for about 3 cm for 5 minutes.

The rooting promoters (IAA, IBA, NAA) were inserted before insertion on May 10, 2008 and August 7, 2008, respectively, ⁰ mgL ^-1 [untreated (control)], 100 mgL ^-1 , 200 ^{㎎L -1, 500㎎L -1, 1000㎎L -1} , respectively, were processed by the concentration of 2000㎎L ^-1, 1- naphthyl acetamide (1-naphthylacetamide 0.4%) ( Rooton) is treated with the powder After insertion into the topsoil.

The above-mentioned soils used for cutting on May 10, 2008 and August 7, 2008, respectively, were mixed with sand: peat moss: vermiculite mixed in a volume ratio of 1: 1: 1.

The results of this study showed that the survival rate and root length were more than 5 mm after 3 months of cutting, and the rooting rate, rooting number, root length, and thickness of each treatment were examined.

The data analysis was used to analyze the variance of the rooting rate characteristics by using the angular transformation for strict analysis of the experimental data (SAS, 1996).

2. Cutting result

The effect of cutting time of the cedar tree on rooting was 0 ~ 43.8% for May cutting, and the average rooting rate was 20.4%. The difference was shown (see FIG. 2).

In particular, all of the uncut roots were killed in August, while the average survival rate was 68% in May, and the second grade was 9 months after the insertion. However, only 27 of the 5.9% were found to have additional rootings, making rooting very slow and difficult compared to other species (see Table 1).

The effects of the rooting promoters on the basis of IAA, IBA, NAA, and Rooton treatment showed the highest rooting rates of 21.7%, 25.4%, 12.7%, and 20.4%, respectively (see FIG. 3). In addition, the coefficient of variation was 34.4%, 32.7%, 58.4%, and 50.0%, respectively, indicating that the lowest value of IBA treatment was more stable than the other treatments.

The effects of rooting promoter concentrations were 30.4%, 14.0% and 20.2 on average in the untreated, 100mgL- ¹ , 200mgL- ¹ , 500mgL- ¹ , 1000mgL- ¹ and 2000mgL- ¹ , respectively. The rooting ratios of%, 26.5%, 22.3%, and 12.7% were shown, which was the best in no treatment (Table 1). In addition, the type and concentration of each other rooting promoter has been the most favorable case in the IBA 1000㎎L ^-1 treated with a mean 36.4%, IAA and NAA is the case both in the concentration 500㎎L ^-1 treated with an average 27.8% and 23.9%, respectively Good rooting rate was shown. Therefore, IBA 1000mgL ^-1 treatment may be the most effective method for asexual growth of Perennial bark by cuttings in May.

As a result of analyzing the effects of the types and concentrations of rooting promoters on rooting, statistical significance was found in the types, concentrations, and interactions of rooting promoters (see Table 2). However, statistical significance between repetitions was not recognized, so the difference in cutting rooting rate between clumps was insignificant.

The characteristics of root number, length and thickness of rooting roots are shown in Table 3 and FIG. 4, and statistical significance was not recognized according to the type and concentration of rooting promoter. The number of roots showed 1.93 average dog, dogs showed a maximum 1.0 minimum in NAA 2000㎎L ^-1 NAA 500㎎L ^-1 2.83 in dogs. By rooting agents, IBA, IAA, NAA, and Rooton showed 1.87, 1.83, 1.94, and 2.20 roots, respectively. Root length ranged from 2.8 to 16.4cm, with an average of 12.5cm in total, showing the best growth of 16.4cm and 16.1cm in IBA and NAA 500mgL- ¹ . Root thickness ranged from 0.61 to 0.93 mm, averaging 0.79 mm.

In conclusion, several factors such as cutting time, type and concentration of rooting promoters affect the rooting of rare trees, which are rare species. It was found that picking and cutting hardwood in April and May was preferable to semi-hardwood. The high rate of callus formation type and concentration of the rooting rooting promoter that promotes the IBA 1000㎎L ^{- 1} and is determined to be a large amount of growth by propagation in air permeability and good drainage baehapto.

Rooting rate and survival rate according to the type and concentration of rooting promoters Rooting accelerator Rooting rate (%) Survival rate
Survival rate (%) Kinds density
(MgL- ¹ ) Replication Average
(Mean) I Ⅱ Ⅲ No treatment
(Control) 0 31.3 25.0 35.0 30.4 82.5 Rooton - 31.3 18.8 11.1 20.4 53.7 IAA 100 25.0 12.5 22.2 19.9 70.6 200 25.0 25.0 22.2 24.1 68.5 500 18.8 31.3 33.3 27.8 67.8 1000 37.5 25.0 11.1 24.5 86.1 2000 0 12.5 5.6 9.1 64.6 IBA 100 18.8 18.8 5.6 14.4 82.2 200 37.5 18.8 5.6 20.6 92.2 500 18.8 31.3 33.0 27.7 94.0 1000 37.5 43.8 27.8 36.4 87.9 2000 31.3 25.0 27.8 28.0 80.3 NAA 100 0 6.3 16.7 11.5 70.1 200 18.8 6.3 22.2 15.8 72.3 500 12.5 31.3 27.8 23.9 62.5 1000 6.3 6.3 5.6 6.1 6.1 2000 6.3 0 0 6.3 6.3

Analysis of Variance on the Rooting Rate of Ripening Bark Trees Variable df Mean square F-value Pr> F Growth regulators (A) 3 361.7 7.27 0.0006 Concentration (B) 5 351.2 7.07 0.0001 Replication 2 4.9 0.10 0.9058 A ㅧ B 10 138.3 2.78 0.0118

Rooting Characteristics According to Cuttings of Persimmon Trees Rooting accelerator Root can
(No. of root)
(EA) Root length
(Root length)
(Cm) Root diameter
(Root diameter) (mm) Kinds density
(MgL- ¹ ) No treatment
(Control) 0 2.36 ± 0.1 Z 12.42 ± 0.7 0.87 ± 0.01 Rooton  - 2.20 ± 1.2 11.93 ± 0.8 0.87 ± 0.13 IAA 100 1.50 ± 0.4 12.99 ± 4.3 0.67 ± 0.04 200 1.92 ± 0.3  9.55 ± 1.6 0.70 ± 0.12 500 2.12 ± 0.1 11.90 ± 4.7 0.69 ± 0.13 1000 1.36 ± 0.4 14.74 ± 0.6 0.89 ± 0.12 2000 2.25 ± 1.1 10.75 ± 0.4 0.61 ± 0.34 IBA 100 1.45 ± 0.4 15.69 ± 4.4 0.81 ± 0.17 200 1.83 ± 1.3  7.39 ± 4.6 0.79 ± 0.25 500 2.09 ± 0.3 16.39 ± 1.6 0.66 ± 0.48 1000 1.62 ± 0.6 13.62 ± 2.7 0.81 ± 0.02 2000 2.38 ± 0.7 13.69 ± 1.7 0.78 ± 0.04 NAA 100 1.50 ± 0.7 12.50 ± 0.0 0.80 ± 0.06 200 1.69 ± 0.7 14.59 ± 4.6 0.73 ± 0.07 500 2.83 ± 0.7 16.09 ± 3.2 0.93 ± 0.12 1000 2.67 ± 2.9 14.60 ± 10.7 0.83 ± 0.43 2000 1.00 ± 0.0  2.80 ± 0.0 0.90 ± 0.00

^Z standard deviation

≪ Example 1 >

On May 15, 2009, a shovel was taken from the native cedar, which grows 1,520 meters above sea level near Mt.

The above inserts were treated with an IBA 1000mgL ^-1 rooting promoter, and then inserted on culture soil mixed with sand: pitmos: vermiculite in a volume ratio of 1: 1: 1: on the same day, to grow the fragrant wood.

The cuts collected from the fragrance trees were 8 cm long and were used to shoot 2 year old fragrance trees.

Treatment of the cuttings collected from the fragrant wood with rooting promoter was immersed for 5 minutes in the rooting agent 3 cm of the base of the cuttings collected from the fragrant wood.

<Example 2>

On May 15, 2009, a shovel was taken from the native cedar, which grows 1,520 meters above sea level near Mt.

The above cuttings were treated with IAA 500mgL ^-1 rooting promoter and then inserted on cultured soil mixed with sand: pitmos: vermiculite in a volume ratio of 1: 1: 1: on the same day, to grow the fragrant wood.

The cuts collected from the fragrance trees were 8 cm long and were used to shoot 2 year old fragrance trees.

Treatment of the cuttings collected from the fragrant wood with rooting promoter was immersed for 5 minutes in the rooting agent 3 cm of the base of the cuttings collected from the fragrant wood.

<Example 3>

On May 15, 2009, a shovel was taken from the native cedar, which grows 1,520 meters above sea level near Mt.

The above inserts were treated with a rooting promoter of NAA 500 mgL ^-1 and then inserted into cultured soil mixed with sand: pitmos: vermiculite in a volume ratio of 1: 1: 1 on the same day to grow the fragrant wood.

The cuts collected from the fragrance trees were 8 cm long and were used to shoot 2 year old fragrance trees.

Treatment of the cuttings collected from the fragrant wood with rooting promoter was immersed for 5 minutes in the rooting agent 3 cm of the base of the cuttings collected from the fragrant wood.

As described above, the present invention has been described with reference to the preferred experimental examples and examples, but those skilled in the art can vary the present invention without departing from the spirit and scope of the present invention as set forth in the claims below. It will be understood that modifications and changes can be made.

Rooting promoter which has higher callus formation rate and promotes rooting compared to the untreated (control) without the rooting agent in the cutting time, the cutting time and the cutting of the fragrance tree in the growth method of the fragrance tree using the cutting of the present invention By identifying the type and concentration of can provide a method for growing perennial tree using the best cutting.

In particular, the method of propagating the fragrant tree using the cutting of the present invention can maintain the genetically identical traits of the fragrant tree because it uses the uncultivated breeding method. It can lead to solving the problem of sexual growth. In addition, the production period of seedlings can be shortened and mass production at a low cost can greatly contribute to the perennial tree-related industry, so there is industrial applicability.

Claims (9)

Treating the cuttings collected from the fragrant wood with a rooting promoter;
The method of growing the fragrant wood using the cuttings, characterized in that the step of inserting the rooting treatment treated with the rooting in the culture soil. The method of claim 1,
Treatment of Iso 1000 mgL ^-1 , IAA 500 mgL ^-1 or NAA 500 mgL ^{-1 with} a rooting promoter, in April-May;
Rootwood growth method using the cuttings comprising the step of inserting the rooting treatment-treated cuttings in the culture soil in April-May. The method of claim 1,
The cuttings of the persimmon tree is 7-10 cm in length and 1--3 year old shoots of the fragrance tree (shallow), characterized in that the fragrance tree growth method using. The method of claim 1,
The cuttings of the fragrance tree are 8 cm long and are used to grow the fragrance of the fragrance tree. The method of claim 1,
A method of propagating fragrance trees using cuttings, characterized in that the treatment of the cuttings collected from the fragrant tree with the rooting agent is immersed in the rooting agent 2.5 ~ 3.5cm base for 3 to 7 minutes. The method of claim 1,
Treatment of the cuttings collected from the fragrant tree with rooting promoter is a method of propagating the fragrant tree using the cuttings, characterized in that immersion of 3cm base of the cuttings collected from the fragrant tree for 5 minutes in the rooting promoter. The method of claim 1,
Cultured soil is a mixture of sand: pitmos: vermiculite is a mixed soil mixed with the same volume ratio of the persimmon tree growth method. The method of claim 1,
Cultured soil is a mixture of sand: pitmos: vermiculite 1: 1: 1: mixed soil, characterized in that the growth of persimmon tree using the cuttings. Claims 1 to 8 of the persimmon tree obtained by the method of growing persimmon tree using the cutting of any one of the selected.

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