CN115558661A - 用于使用模板转换反应进行cDNA合成和单细胞转录组概况分析的方法和组合物 - Google Patents

用于使用模板转换反应进行cDNA合成和单细胞转录组概况分析的方法和组合物 Download PDF

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CN115558661A
CN115558661A CN202211158053.7A CN202211158053A CN115558661A CN 115558661 A CN115558661 A CN 115558661A CN 202211158053 A CN202211158053 A CN 202211158053A CN 115558661 A CN115558661 A CN 115558661A
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cdna
tso
rna
nucleotide residue
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里卡德·桑德伯格
西莫内·佩斯里
欧麦德·R·法瑞达尼
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Ludwig Institute for Cancer Research Ltd
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Ludwig Institute for Cancer Research Ltd
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CN202211158053.7A 2013-08-23 2014-08-22 用于使用模板转换反应进行cDNA合成和单细胞转录组概况分析的方法和组合物 Pending CN115558661A (zh)

Applications Claiming Priority (4)

Application Number Priority Date Filing Date Title
US201361869220P 2013-08-23 2013-08-23
US61/869,220 2013-08-23
CN201480053360.6A CN105579587A (zh) 2013-08-23 2014-08-22 用于使用模板转换反应进行cDNA合成和单细胞转录组概况分析的方法和组合物
PCT/US2014/052233 WO2015027135A1 (en) 2013-08-23 2014-08-22 Methods and compositions for cdna synthesis and single-cell transcriptome profiling using template switching reaction

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CN201480053360.6A Pending CN105579587A (zh) 2013-08-23 2014-08-22 用于使用模板转换反应进行cDNA合成和单细胞转录组概况分析的方法和组合物

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CA (1) CA2921603C (enExample)
DK (1) DK3036336T3 (enExample)
ES (1) ES2827307T3 (enExample)
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WO2015027135A1 (en) * 2013-08-23 2015-02-26 Ludwig Institute For Cancer Research Methods and compositions for cdna synthesis and single-cell transcriptome profiling using template switching reaction
CN106460033B (zh) * 2013-12-30 2021-12-24 阿特雷卡公司 使用核酸条形码分析与单细胞缔合的核酸
EP4461812A3 (en) 2015-09-15 2025-01-22 Takara Bio USA, Inc. Method for preparing a next generation sequencing (ngs) library from a ribonucleic acid (rna) sample and compositions for practicing the same
JP7064439B2 (ja) 2015-11-04 2022-05-10 アトレカ インコーポレイテッド 単一細胞に関連する核酸の解析のための、核酸バーコードの組み合わせセット
WO2017214417A1 (en) 2016-06-10 2017-12-14 Takara Bio Usa, Inc. Methods and compositions employing blocked primers
CN106498040B (zh) * 2016-10-12 2018-10-23 浙江大学 一种分子标记微珠及基于该分子标记微珠的高通量单细胞测序方法
EP3985122A1 (en) 2016-11-10 2022-04-20 Takara Bio USA, Inc. Methods of producing amplified double stranded deoxyribonucleic acids and compositions and kits for use therein
CN110312800B (zh) 2016-11-11 2025-01-10 生物辐射实验室股份有限公司 处理核酸样本的方法
CN106868005B (zh) * 2017-03-17 2020-07-21 中山大学附属肿瘤医院 一种用于高效快速扩增cDNA末端的锚定引物及扩增方法
US11946043B2 (en) 2017-09-11 2024-04-02 Ludwig Institute For Cancer Research Ltd Selective labeling of 5-methylcytosine in circulating cell-free DNA
CN108103055B (zh) * 2018-01-09 2021-05-28 序康医疗科技（苏州）有限公司 一种单细胞rna逆转录与文库构建的方法
US11814674B2 (en) 2018-02-02 2023-11-14 The United States Of America, As Represented By The Secretary Department Of Health And Human Services Random amplification methods for extremely low input nucleic acids
WO2020036926A1 (en) 2018-08-17 2020-02-20 Cellecta, Inc. Multiplex preparation of barcoded gene specific dna fragments
WO2020136438A1 (en) 2018-12-28 2020-07-02 Biobloxx Ab Method and kit for preparing complementary dna
CN113811610A (zh) * 2019-05-09 2021-12-17 加利福尼亚太平洋生物科学股份有限公司 用于改进cDNA合成的组合物和方法
EP3998338B1 (en) 2019-07-11 2024-04-24 Tokyo University of Science Foundation Method for amplifying nucleic acid using solid-phase carrier
WO2021236963A1 (en) * 2020-05-20 2021-11-25 Chan Zuckerberg Biohub, Inc. Total rna profiling of biological samples and single cells
WO2023241721A1 (zh) * 2022-06-17 2023-12-21 复旦大学 基于拓扑捕获的rna样本处理系统
WO2024185884A1 (ja) 2023-03-09 2024-09-12 イムノジェネテクス株式会社 相補ｄｎａ鎖を増幅する方法
CN116497105B (zh) * 2023-06-28 2023-09-29 浙江大学 基于末端转移酶的单细胞转录组测序试剂盒及测序方法
CN119776495A (zh) * 2024-12-31 2025-04-08 北京普译生物科技有限公司 一种cDNA建库中的模板转换方法

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US20190264284A1 (en) 2019-08-29
JP2018088924A (ja) 2018-06-14
DK3036336T3 (da) 2020-11-02
EP3036336A4 (en) 2017-06-28
CA2921603A1 (en) 2015-02-26
JP6603699B2 (ja) 2019-11-06
CN105579587A (zh) 2016-05-11
ES2827307T3 (es) 2021-05-20
EP3036336B1 (en) 2020-09-30
CA2921603C (en) 2019-11-05
US10266894B2 (en) 2019-04-23
EP3036336A1 (en) 2016-06-29
US20160258016A1 (en) 2016-09-08
JP2016527919A (ja) 2016-09-15
WO2015027135A1 (en) 2015-02-26
JP6336080B2 (ja) 2018-06-06

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