CA2921603C - Methods and compositions for cdna synthesis and single-cell transcriptome profiling using template switching reaction - Google Patents
Methods and compositions for cdna synthesis and single-cell transcriptome profiling using template switching reaction Download PDFInfo
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- CA2921603C CA2921603C CA2921603A CA2921603A CA2921603C CA 2921603 C CA2921603 C CA 2921603C CA 2921603 A CA2921603 A CA 2921603A CA 2921603 A CA2921603 A CA 2921603A CA 2921603 C CA2921603 C CA 2921603C
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Classifications
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
- C12Q1/6883—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for diseases caused by alterations of genetic material
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
-
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- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
- C12N15/1096—Processes for the isolation, preparation or purification of DNA or RNA cDNA Synthesis; Subtracted cDNA library construction, e.g. RT, RT-PCR
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- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P19/00—Preparation of compounds containing saccharide radicals
- C12P19/26—Preparation of nitrogen-containing carbohydrates
- C12P19/28—N-glycosides
- C12P19/30—Nucleotides
- C12P19/34—Polynucleotides, e.g. nucleic acids, oligoribonucleotides
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6809—Methods for determination or identification of nucleic acids involving differential detection
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- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6844—Nucleic acid amplification reactions
- C12Q1/6853—Nucleic acid amplification reactions using modified primers or templates
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- C—CHEMISTRY; METALLURGY
- C40—COMBINATORIAL TECHNOLOGY
- C40B—COMBINATORIAL CHEMISTRY; LIBRARIES, e.g. CHEMICAL LIBRARIES
- C40B40/00—Libraries per se, e.g. arrays, mixtures
- C40B40/04—Libraries containing only organic compounds
- C40B40/06—Libraries containing nucleotides or polynucleotides, or derivatives thereof
- C40B40/08—Libraries containing RNA or DNA which encodes proteins, e.g. gene libraries
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- C—CHEMISTRY; METALLURGY
- C40—COMBINATORIAL TECHNOLOGY
- C40B—COMBINATORIAL CHEMISTRY; LIBRARIES, e.g. CHEMICAL LIBRARIES
- C40B50/00—Methods of creating libraries, e.g. combinatorial synthesis
- C40B50/06—Biochemical methods, e.g. using enzymes or whole viable microorganisms
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q2600/00—Oligonucleotides characterized by their use
- C12Q2600/158—Expression markers
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| US201361869220P | 2013-08-23 | 2013-08-23 | |
| US61/869,220 | 2013-08-23 | ||
| PCT/US2014/052233 WO2015027135A1 (en) | 2013-08-23 | 2014-08-22 | Methods and compositions for cdna synthesis and single-cell transcriptome profiling using template switching reaction |
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| CA2921603A1 CA2921603A1 (en) | 2015-02-26 |
| CA2921603C true CA2921603C (en) | 2019-11-05 |
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| CN105579587A (zh) * | 2013-08-23 | 2016-05-11 | 惠氏公司 | 用于使用模板转换反应进行cDNA合成和单细胞转录组概况分析的方法和组合物 |
| PL3089822T3 (pl) * | 2013-12-30 | 2022-09-19 | Atreca, Inc. | Analiza kwasów nukleinowych powiązanych z pojedynczymi komórkami z użyciem kodów kreskowych kwasów nukleinowych |
| EP4461812A3 (en) | 2015-09-15 | 2025-01-22 | Takara Bio USA, Inc. | Method for preparing a next generation sequencing (ngs) library from a ribonucleic acid (rna) sample and compositions for practicing the same |
| CA3004310A1 (en) | 2015-11-04 | 2017-05-11 | Atreca, Inc. | Combinatorial sets of nucleic acid barcodes for analysis of nucleic acids associated with single cells |
| WO2017214417A1 (en) | 2016-06-10 | 2017-12-14 | Takara Bio Usa, Inc. | Methods and compositions employing blocked primers |
| CN106498040B (zh) * | 2016-10-12 | 2018-10-23 | 浙江大学 | 一种分子标记微珠及基于该分子标记微珠的高通量单细胞测序方法 |
| JP7050057B2 (ja) | 2016-11-10 | 2022-04-07 | タカラ バイオ ユーエスエー， インコーポレイテッド | 増幅二本鎖デオキシリボ核酸の作製方法ならびに該方法で使用する組成物及びキット |
| JP7155136B2 (ja) | 2016-11-11 | 2022-10-18 | バイオ－ラド ラボラトリーズ，インコーポレイティド | 核酸試料をプロセシングする方法 |
| CN106868005B (zh) * | 2017-03-17 | 2020-07-21 | 中山大学附属肿瘤医院 | 一种用于高效快速扩增cDNA末端的锚定引物及扩增方法 |
| EP3682005A4 (en) | 2017-09-11 | 2021-05-26 | Ludwig Institute for Cancer Research Ltd. | SELECTIVE LABELING OF 5-METHYLCYTOSINE IN CIRCULATING CELL-FREE DNA |
| CN108103055B (zh) * | 2018-01-09 | 2021-05-28 | 序康医疗科技（苏州）有限公司 | 一种单细胞rna逆转录与文库构建的方法 |
| US11814674B2 (en) | 2018-02-02 | 2023-11-14 | The United States Of America, As Represented By The Secretary Department Of Health And Human Services | Random amplification methods for extremely low input nucleic acids |
| EP3837380A4 (en) | 2018-08-17 | 2022-05-11 | Cellecta, Inc. | MULTIPLEX PREPARATION OF BARCODE GENE-SPECIFIC DNA FRAGMENTS |
| WO2020136438A1 (en) | 2018-12-28 | 2020-07-02 | Biobloxx Ab | Method and kit for preparing complementary dna |
| CN113811610A (zh) * | 2019-05-09 | 2021-12-17 | 加利福尼亚太平洋生物科学股份有限公司 | 用于改进cDNA合成的组合物和方法 |
| EP3998338B1 (en) | 2019-07-11 | 2024-04-24 | Tokyo University of Science Foundation | Method for amplifying nucleic acid using solid-phase carrier |
| US20230193254A1 (en) * | 2020-05-20 | 2023-06-22 | Chan Zuckerberg Biohub, Inc. | Total rna profiling of biological samples and single cells |
| WO2023241721A1 (zh) * | 2022-06-17 | 2023-12-21 | 复旦大学 | 基于拓扑捕获的rna样本处理系统 |
| AU2024231805A1 (en) | 2023-03-09 | 2025-09-18 | Immunogeneteqs, Inc. | Method for amplifying complimentary dna strand |
| CN116497105B (zh) * | 2023-06-28 | 2023-09-29 | 浙江大学 | 基于末端转移酶的单细胞转录组测序试剂盒及测序方法 |
| CN119776495A (zh) * | 2024-12-31 | 2025-04-08 | 北京普译生物科技有限公司 | 一种cDNA建库中的模板转换方法 |
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| US5962271A (en) * | 1996-01-03 | 1999-10-05 | Cloutech Laboratories, Inc. | Methods and compositions for generating full-length cDNA having arbitrary nucleotide sequence at the 3'-end |
| CN1350591A (zh) | 1999-03-18 | 2002-05-22 | 埃克西库恩公司 | 以单一步骤制备和检测复合生物样品中的核酸 |
| DK3002337T3 (en) * | 2009-03-30 | 2019-02-18 | Illumina Inc | ANALYSIS OF EXPRESSION OF GENES IN SINGLE CELLS |
| EP3037536B1 (en) * | 2011-01-28 | 2019-11-27 | Illumina, Inc. | Oligonucleotide replacement for di-tagged and directional libraries |
| EP2670894B1 (en) | 2011-02-02 | 2017-11-29 | University Of Washington Through Its Center For Commercialization | Massively parallel continguity mapping |
| WO2012129363A2 (en) | 2011-03-24 | 2012-09-27 | President And Fellows Of Harvard College | Single cell nucleic acid detection and analysis |
| CN105579587A (zh) * | 2013-08-23 | 2016-05-11 | 惠氏公司 | 用于使用模板转换反应进行cDNA合成和单细胞转录组概况分析的方法和组合物 |
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| US10266894B2 (en) | 2019-04-23 |
| JP2018088924A (ja) | 2018-06-14 |
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| JP6336080B2 (ja) | 2018-06-06 |
| ES2827307T3 (es) | 2021-05-20 |
| US20160258016A1 (en) | 2016-09-08 |
| CN105579587A (zh) | 2016-05-11 |
| DK3036336T3 (da) | 2020-11-02 |
| US20190264284A1 (en) | 2019-08-29 |
| EP3036336A1 (en) | 2016-06-29 |
| CA2921603A1 (en) | 2015-02-26 |
| JP6603699B2 (ja) | 2019-11-06 |
| EP3036336B1 (en) | 2020-09-30 |
| CN115558661A (zh) | 2023-01-03 |
| JP2016527919A (ja) | 2016-09-15 |
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