DK3036336T3 - Fremgangsmåder og sammensætninger til cdna-syntese og enkeltcelle transkriptomprofilering under anvendelse af skabelonomskiftende reaktion - Google Patents
Fremgangsmåder og sammensætninger til cdna-syntese og enkeltcelle transkriptomprofilering under anvendelse af skabelonomskiftende reaktion Download PDFInfo
- Publication number
- DK3036336T3 DK3036336T3 DK14837396.2T DK14837396T DK3036336T3 DK 3036336 T3 DK3036336 T3 DK 3036336T3 DK 14837396 T DK14837396 T DK 14837396T DK 3036336 T3 DK3036336 T3 DK 3036336T3
- Authority
- DK
- Denmark
- Prior art keywords
- propholfing
- compositions
- methods
- single cell
- cdna synthesis
- Prior art date
Links
- 238000010804 cDNA synthesis Methods 0.000 title 1
- 238000006243 chemical reaction Methods 0.000 title 1
- 238000000034 method Methods 0.000 title 1
- 239000000203 mixture Substances 0.000 title 1
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
- C12Q1/6883—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for diseases caused by alterations of genetic material
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6809—Methods for determination or identification of nucleic acids involving differential detection
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
- C12N15/1096—Processes for the isolation, preparation or purification of DNA or RNA cDNA Synthesis; Subtracted cDNA library construction, e.g. RT, RT-PCR
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P19/00—Preparation of compounds containing saccharide radicals
- C12P19/26—Preparation of nitrogen-containing carbohydrates
- C12P19/28—N-glycosides
- C12P19/30—Nucleotides
- C12P19/34—Polynucleotides, e.g. nucleic acids, oligoribonucleotides
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6844—Nucleic acid amplification reactions
- C12Q1/6853—Nucleic acid amplification reactions using modified primers or templates
-
- C—CHEMISTRY; METALLURGY
- C40—COMBINATORIAL TECHNOLOGY
- C40B—COMBINATORIAL CHEMISTRY; LIBRARIES, e.g. CHEMICAL LIBRARIES
- C40B40/00—Libraries per se, e.g. arrays, mixtures
- C40B40/04—Libraries containing only organic compounds
- C40B40/06—Libraries containing nucleotides or polynucleotides, or derivatives thereof
- C40B40/08—Libraries containing RNA or DNA which encodes proteins, e.g. gene libraries
-
- C—CHEMISTRY; METALLURGY
- C40—COMBINATORIAL TECHNOLOGY
- C40B—COMBINATORIAL CHEMISTRY; LIBRARIES, e.g. CHEMICAL LIBRARIES
- C40B50/00—Methods of creating libraries, e.g. combinatorial synthesis
- C40B50/06—Biochemical methods, e.g. using enzymes or whole viable microorganisms
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q2600/00—Oligonucleotides characterized by their use
- C12Q2600/158—Expression markers
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- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Organic Chemistry (AREA)
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Wood Science & Technology (AREA)
- Zoology (AREA)
- Genetics & Genomics (AREA)
- Molecular Biology (AREA)
- General Engineering & Computer Science (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Biotechnology (AREA)
- Biochemistry (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Microbiology (AREA)
- General Health & Medical Sciences (AREA)
- Analytical Chemistry (AREA)
- Biophysics (AREA)
- Physics & Mathematics (AREA)
- Biomedical Technology (AREA)
- Immunology (AREA)
- Chemical Kinetics & Catalysis (AREA)
- General Chemical & Material Sciences (AREA)
- Plant Pathology (AREA)
- Medicinal Chemistry (AREA)
- Pathology (AREA)
- Bioinformatics & Computational Biology (AREA)
- Crystallography & Structural Chemistry (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US201361869220P | 2013-08-23 | 2013-08-23 | |
| PCT/US2014/052233 WO2015027135A1 (en) | 2013-08-23 | 2014-08-22 | Methods and compositions for cdna synthesis and single-cell transcriptome profiling using template switching reaction |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| DK3036336T3 true DK3036336T3 (da) | 2020-11-02 |
Family
ID=52484185
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| DK14837396.2T DK3036336T3 (da) | 2013-08-23 | 2014-08-22 | Fremgangsmåder og sammensætninger til cdna-syntese og enkeltcelle transkriptomprofilering under anvendelse af skabelonomskiftende reaktion |
Country Status (8)
| Country | Link |
|---|---|
| US (2) | US10266894B2 (enExample) |
| EP (1) | EP3036336B1 (enExample) |
| JP (2) | JP6336080B2 (enExample) |
| CN (2) | CN115558661A (enExample) |
| CA (1) | CA2921603C (enExample) |
| DK (1) | DK3036336T3 (enExample) |
| ES (1) | ES2827307T3 (enExample) |
| WO (1) | WO2015027135A1 (enExample) |
Families Citing this family (21)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN115558661A (zh) * | 2013-08-23 | 2023-01-03 | 路德维格癌症研究院 | 用于使用模板转换反应进行cDNA合成和单细胞转录组概况分析的方法和组合物 |
| SG11201605344YA (en) * | 2013-12-30 | 2016-07-28 | Atreca Inc | Analysis of nucleic acids associated with single cells using nucleic acid barcodes |
| EP3350732B1 (en) | 2015-09-15 | 2024-07-24 | Takara Bio USA, Inc. | Method for preparing a next generation sequencing (ngs) library from a ribonucleic acid (rna) sample and kit for practicing the same |
| CN108473984A (zh) | 2015-11-04 | 2018-08-31 | 阿特雷卡公司 | 用于分析与单个细胞相关联的核酸的核酸条形码的组合组 |
| WO2017214417A1 (en) | 2016-06-10 | 2017-12-14 | Takara Bio Usa, Inc. | Methods and compositions employing blocked primers |
| CN106498040B (zh) * | 2016-10-12 | 2018-10-23 | 浙江大学 | 一种分子标记微珠及基于该分子标记微珠的高通量单细胞测序方法 |
| EP3538662B1 (en) | 2016-11-10 | 2021-12-01 | Takara Bio USA, Inc. | Methods of producing amplified double stranded deoxyribonucleic acids and compositions and kits for use therein |
| KR102489902B1 (ko) | 2016-11-11 | 2023-01-19 | 바이오 래드 래버러토리스 인코오포레이티드 | 핵산 샘플을 프로세싱하는 방법 |
| CN106868005B (zh) * | 2017-03-17 | 2020-07-21 | 中山大学附属肿瘤医院 | 一种用于高效快速扩增cDNA末端的锚定引物及扩增方法 |
| CA3075280A1 (en) | 2017-09-11 | 2019-03-14 | Ludwig Institute For Cancer Research Ltd | Selective labeling of 5-methylcytosine in circulating cell-free dna |
| CN108103055B (zh) * | 2018-01-09 | 2021-05-28 | 序康医疗科技（苏州）有限公司 | 一种单细胞rna逆转录与文库构建的方法 |
| WO2019152860A1 (en) | 2018-02-02 | 2019-08-08 | The United States Of America, As Represented By The Secretary, Department Of Health And Human Services | Random amplification methods for extremely low input nucleic acids |
| EP3837380A4 (en) | 2018-08-17 | 2022-05-11 | Cellecta, Inc. | MULTIPLEX PREPARATION OF BARCODE GENE-SPECIFIC DNA FRAGMENTS |
| EP3902922A1 (en) | 2018-12-28 | 2021-11-03 | Biobloxx AB | Method and kit for preparing complementary dna |
| CN113811610A (zh) * | 2019-05-09 | 2021-12-17 | 加利福尼亚太平洋生物科学股份有限公司 | 用于改进cDNA合成的组合物和方法 |
| US20220356462A1 (en) | 2019-07-11 | 2022-11-10 | Tokyo University Of Science Foundation | Method for amplifying nucleic acid using solid-phase carrier |
| US20230193254A1 (en) * | 2020-05-20 | 2023-06-22 | Chan Zuckerberg Biohub, Inc. | Total rna profiling of biological samples and single cells |
| CN117247990A (zh) * | 2022-06-17 | 2023-12-19 | 复旦大学 | 基于拓扑捕获的rna样本处理系统 |
| KR20250094720A (ko) | 2023-03-09 | 2025-06-25 | 이뮤노제네텍스 가부시키가이샤 | 상보 dna쇄를 증폭하는 방법 |
| CN116497105B (zh) * | 2023-06-28 | 2023-09-29 | 浙江大学 | 基于末端转移酶的单细胞转录组测序试剂盒及测序方法 |
| CN119776495A (zh) * | 2024-12-31 | 2025-04-08 | 北京普译生物科技有限公司 | 一种cDNA建库中的模板转换方法 |
Family Cites Families (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US5962271A (en) | 1996-01-03 | 1999-10-05 | Cloutech Laboratories, Inc. | Methods and compositions for generating full-length cDNA having arbitrary nucleotide sequence at the 3'-end |
| EP1161561B1 (en) | 1999-03-18 | 2005-12-28 | Exiqon A/S | One step sample preparation and detection of nucleic acids in complex biological samples |
| US20120010091A1 (en) * | 2009-03-30 | 2012-01-12 | Illumina, Inc. | Gene expression analysis in single cells |
| DK3037536T3 (da) | 2011-01-28 | 2020-01-13 | Illumina Inc | Oligonukleotiderstatning for di-taggede og retnings biblioteker |
| EP2670894B1 (en) * | 2011-02-02 | 2017-11-29 | University Of Washington Through Its Center For Commercialization | Massively parallel continguity mapping |
| WO2012129363A2 (en) * | 2011-03-24 | 2012-09-27 | President And Fellows Of Harvard College | Single cell nucleic acid detection and analysis |
| CN115558661A (zh) * | 2013-08-23 | 2023-01-03 | 路德维格癌症研究院 | 用于使用模板转换反应进行cDNA合成和单细胞转录组概况分析的方法和组合物 |
-
2014
- 2014-08-22 CN CN202211158053.7A patent/CN115558661A/zh active Pending
- 2014-08-22 US US14/912,556 patent/US10266894B2/en active Active
- 2014-08-22 EP EP14837396.2A patent/EP3036336B1/en active Active
- 2014-08-22 JP JP2016536474A patent/JP6336080B2/ja active Active
- 2014-08-22 CA CA2921603A patent/CA2921603C/en active Active
- 2014-08-22 ES ES14837396T patent/ES2827307T3/es active Active
- 2014-08-22 CN CN201480053360.6A patent/CN105579587A/zh active Pending
- 2014-08-22 DK DK14837396.2T patent/DK3036336T3/da active
- 2014-08-22 WO PCT/US2014/052233 patent/WO2015027135A1/en not_active Ceased
-
2017
- 2017-12-28 JP JP2017253460A patent/JP6603699B2/ja active Active
-
2019
- 2019-02-19 US US16/279,245 patent/US20190264284A1/en not_active Abandoned
Also Published As
| Publication number | Publication date |
|---|---|
| EP3036336B1 (en) | 2020-09-30 |
| CA2921603C (en) | 2019-11-05 |
| US10266894B2 (en) | 2019-04-23 |
| EP3036336A4 (en) | 2017-06-28 |
| CN105579587A (zh) | 2016-05-11 |
| EP3036336A1 (en) | 2016-06-29 |
| JP2016527919A (ja) | 2016-09-15 |
| CN115558661A (zh) | 2023-01-03 |
| JP2018088924A (ja) | 2018-06-14 |
| JP6336080B2 (ja) | 2018-06-06 |
| JP6603699B2 (ja) | 2019-11-06 |
| WO2015027135A1 (en) | 2015-02-26 |
| CA2921603A1 (en) | 2015-02-26 |
| ES2827307T3 (es) | 2021-05-20 |
| US20190264284A1 (en) | 2019-08-29 |
| US20160258016A1 (en) | 2016-09-08 |
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