ES2827307T3 - Métodos y composiciones para la síntesis de ADNc y la elaboración del perfil del transcriptoma de células individuales utilizando una reacción de cambio de plantilla - Google Patents

Métodos y composiciones para la síntesis de ADNc y la elaboración del perfil del transcriptoma de células individuales utilizando una reacción de cambio de plantilla Download PDF

Info

Publication number
ES2827307T3
ES2827307T3 ES14837396T ES14837396T ES2827307T3 ES 2827307 T3 ES2827307 T3 ES 2827307T3 ES 14837396 T ES14837396 T ES 14837396T ES 14837396 T ES14837396 T ES 14837396T ES 2827307 T3 ES2827307 T3 ES 2827307T3
Authority
ES
Spain
Prior art keywords
cdna
tso
rna
blocked
complementary
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Active
Application number
ES14837396T
Other languages
English (en)
Spanish (es)
Inventor
Rickard Sandberg
Simone Picelli
Omid R Faridani
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Ludwig Institute for Cancer Research Ltd
Original Assignee
Ludwig Institute for Cancer Research Ltd
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Ludwig Institute for Cancer Research Ltd filed Critical Ludwig Institute for Cancer Research Ltd
Application granted granted Critical
Publication of ES2827307T3 publication Critical patent/ES2827307T3/es
Active legal-status Critical Current
Anticipated expiration legal-status Critical

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
    • C12Q1/6883—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for diseases caused by alterations of genetic material
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6809—Methods for determination or identification of nucleic acids involving differential detection
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1096—Processes for the isolation, preparation or purification of DNA or RNA cDNA Synthesis; Subtracted cDNA library construction, e.g. RT, RT-PCR
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P19/00—Preparation of compounds containing saccharide radicals
    • C12P19/26—Preparation of nitrogen-containing carbohydrates
    • C12P19/28—N-glycosides
    • C12P19/30—Nucleotides
    • C12P19/34—Polynucleotides, e.g. nucleic acids, oligoribonucleotides
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/6853—Nucleic acid amplification reactions using modified primers or templates
    • C—CHEMISTRY; METALLURGY
    • C40—COMBINATORIAL TECHNOLOGY
    • C40B—COMBINATORIAL CHEMISTRY; LIBRARIES, e.g. CHEMICAL LIBRARIES
    • C40B40/00—Libraries per se, e.g. arrays, mixtures
    • C40B40/04—Libraries containing only organic compounds
    • C40B40/06—Libraries containing nucleotides or polynucleotides, or derivatives thereof
    • C40B40/08—Libraries containing RNA or DNA which encodes proteins, e.g. gene libraries
    • C—CHEMISTRY; METALLURGY
    • C40—COMBINATORIAL TECHNOLOGY
    • C40B—COMBINATORIAL CHEMISTRY; LIBRARIES, e.g. CHEMICAL LIBRARIES
    • C40B50/00—Methods of creating libraries, e.g. combinatorial synthesis
    • C40B50/06—Biochemical methods, e.g. using enzymes or whole viable microorganisms
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2600/00—Oligonucleotides characterized by their use
    • C12Q2600/158—Expression markers

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Genetics & Genomics (AREA)
  • Molecular Biology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biotechnology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biochemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Microbiology (AREA)
  • General Health & Medical Sciences (AREA)
  • Analytical Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Biomedical Technology (AREA)
  • Immunology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Plant Pathology (AREA)
  • Medicinal Chemistry (AREA)
  • Pathology (AREA)
  • Bioinformatics & Computational Biology (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
ES14837396T 2013-08-23 2014-08-22 Métodos y composiciones para la síntesis de ADNc y la elaboración del perfil del transcriptoma de células individuales utilizando una reacción de cambio de plantilla Active ES2827307T3 (es)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US201361869220P 2013-08-23 2013-08-23
PCT/US2014/052233 WO2015027135A1 (en) 2013-08-23 2014-08-22 Methods and compositions for cdna synthesis and single-cell transcriptome profiling using template switching reaction

Publications (1)

Publication Number Publication Date
ES2827307T3 true ES2827307T3 (es) 2021-05-20

Family

ID=52484185

Family Applications (1)

Application Number Title Priority Date Filing Date
ES14837396T Active ES2827307T3 (es) 2013-08-23 2014-08-22 Métodos y composiciones para la síntesis de ADNc y la elaboración del perfil del transcriptoma de células individuales utilizando una reacción de cambio de plantilla

Country Status (8)

Country Link
US (2) US10266894B2 (enExample)
EP (1) EP3036336B1 (enExample)
JP (2) JP6336080B2 (enExample)
CN (2) CN115558661A (enExample)
CA (1) CA2921603C (enExample)
DK (1) DK3036336T3 (enExample)
ES (1) ES2827307T3 (enExample)
WO (1) WO2015027135A1 (enExample)

Families Citing this family (21)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2015027135A1 (en) * 2013-08-23 2015-02-26 Ludwig Institute For Cancer Research Methods and compositions for cdna synthesis and single-cell transcriptome profiling using template switching reaction
CN106460033B (zh) * 2013-12-30 2021-12-24 阿特雷卡公司 使用核酸条形码分析与单细胞缔合的核酸
EP4461812A3 (en) 2015-09-15 2025-01-22 Takara Bio USA, Inc. Method for preparing a next generation sequencing (ngs) library from a ribonucleic acid (rna) sample and compositions for practicing the same
JP7064439B2 (ja) 2015-11-04 2022-05-10 アトレカ インコーポレイテッド 単一細胞に関連する核酸の解析のための、核酸バーコードの組み合わせセット
WO2017214417A1 (en) 2016-06-10 2017-12-14 Takara Bio Usa, Inc. Methods and compositions employing blocked primers
CN106498040B (zh) * 2016-10-12 2018-10-23 浙江大学 一种分子标记微珠及基于该分子标记微珠的高通量单细胞测序方法
EP3985122A1 (en) 2016-11-10 2022-04-20 Takara Bio USA, Inc. Methods of producing amplified double stranded deoxyribonucleic acids and compositions and kits for use therein
CN110312800B (zh) 2016-11-11 2025-01-10 生物辐射实验室股份有限公司 处理核酸样本的方法
CN106868005B (zh) * 2017-03-17 2020-07-21 中山大学附属肿瘤医院 一种用于高效快速扩增cDNA末端的锚定引物及扩增方法
US11946043B2 (en) 2017-09-11 2024-04-02 Ludwig Institute For Cancer Research Ltd Selective labeling of 5-methylcytosine in circulating cell-free DNA
CN108103055B (zh) * 2018-01-09 2021-05-28 序康医疗科技（苏州）有限公司 一种单细胞rna逆转录与文库构建的方法
US11814674B2 (en) 2018-02-02 2023-11-14 The United States Of America, As Represented By The Secretary Department Of Health And Human Services Random amplification methods for extremely low input nucleic acids
WO2020036926A1 (en) 2018-08-17 2020-02-20 Cellecta, Inc. Multiplex preparation of barcoded gene specific dna fragments
WO2020136438A1 (en) 2018-12-28 2020-07-02 Biobloxx Ab Method and kit for preparing complementary dna
CN113811610A (zh) * 2019-05-09 2021-12-17 加利福尼亚太平洋生物科学股份有限公司 用于改进cDNA合成的组合物和方法
EP3998338B1 (en) 2019-07-11 2024-04-24 Tokyo University of Science Foundation Method for amplifying nucleic acid using solid-phase carrier
WO2021236963A1 (en) * 2020-05-20 2021-11-25 Chan Zuckerberg Biohub, Inc. Total rna profiling of biological samples and single cells
WO2023241721A1 (zh) * 2022-06-17 2023-12-21 复旦大学 基于拓扑捕获的rna样本处理系统
WO2024185884A1 (ja) 2023-03-09 2024-09-12 イムノジェネテクス株式会社 相補ｄｎａ鎖を増幅する方法
CN116497105B (zh) * 2023-06-28 2023-09-29 浙江大学 基于末端转移酶的单细胞转录组测序试剂盒及测序方法
CN119776495A (zh) * 2024-12-31 2025-04-08 北京普译生物科技有限公司 一种cDNA建库中的模板转换方法

Family Cites Families (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5962271A (en) 1996-01-03 1999-10-05 Cloutech Laboratories, Inc. Methods and compositions for generating full-length cDNA having arbitrary nucleotide sequence at the 3'-end
JP4975905B2 (ja) 1999-03-18 2012-07-11 エクシコン エ／エス 複合生物試料における核酸の単工程調製及び検出
ES2706227T3 (es) 2009-03-30 2019-03-27 Illumina Inc Análisis de expresión génica en células individuales
ES2762866T3 (es) 2011-01-28 2020-05-26 Illumina Inc Reemplazo de nucleótidos por bibliotecas doblemente etiquetadas y direccionales
JP6017458B2 (ja) 2011-02-02 2016-11-02 ユニヴァーシティ・オブ・ワシントン・スルー・イッツ・センター・フォー・コマーシャリゼーション 大量並列連続性マッピング
US9260753B2 (en) * 2011-03-24 2016-02-16 President And Fellows Of Harvard College Single cell nucleic acid detection and analysis
WO2015027135A1 (en) * 2013-08-23 2015-02-26 Ludwig Institute For Cancer Research Methods and compositions for cdna synthesis and single-cell transcriptome profiling using template switching reaction

Also Published As

Publication number Publication date
US20190264284A1 (en) 2019-08-29
JP2018088924A (ja) 2018-06-14
DK3036336T3 (da) 2020-11-02
EP3036336A4 (en) 2017-06-28
CA2921603A1 (en) 2015-02-26
JP6603699B2 (ja) 2019-11-06
CN105579587A (zh) 2016-05-11
EP3036336B1 (en) 2020-09-30
CN115558661A (zh) 2023-01-03
CA2921603C (en) 2019-11-05
US10266894B2 (en) 2019-04-23
EP3036336A1 (en) 2016-06-29
US20160258016A1 (en) 2016-09-08
JP2016527919A (ja) 2016-09-15
WO2015027135A1 (en) 2015-02-26
JP6336080B2 (ja) 2018-06-06

Similar Documents

Publication Publication Date Title
ES2827307T3 (es) Métodos y composiciones para la síntesis de ADNc y la elaboración del perfil del transcriptoma de células individuales utilizando una reacción de cambio de plantilla
US10711269B2 (en) Method for making an asymmetrically-tagged sequencing library
ES2959047T3 (es) Bibliotecas de genoma completo de célula individual para la secuenciación de metilación
ES2885191T3 (es) Métodos y composiciones para preparar bibliotecas de ácidos nucleicos
KR102435352B1 (ko) 리가제-연관 핵산 원형화 및 증폭
ES2841077T3 (es) Enriquecimiento de dianas por extensión del cebador de sonda individual
US20200139335A1 (en) Enrichment of DNA Sequencing Libraries from Samples Containing Small Amounts of Target DNA
US20120003657A1 (en) Targeted sequencing library preparation by genomic dna circularization
JP2016535979A (ja) Ｄｎａ末端修復、アデニル化、リン酸化のための酵素組成物
WO2011161549A2 (en) Methods and compositions for polynucleotide library production, immortalization and region of interest extraction
US20190169603A1 (en) Compositions and Methods for Labeling Target Nucleic Acid Molecules
EP3189157A1 (en) Preparation of adapter-ligated amplicons
US20230304069A1 (en) Methods and compositions for single cell analysis
US20220380839A1 (en) Methods and kits for depleting undesired nucleic acids
US20200140850A1 (en) Methods for isolation and quantification of short nucleic acid molecules
KR20150090352A (ko) 염기서열 확인 과정에서 분리된 핵산 단편들을 회수하는 방법
CN112996925A (zh) 用于crispr的靶标无关的向导rna
US20240384336A1 (en) Optimized Set Of Oligonucleotides For Bulk RNA Barcoding And Sequencing
EP4536854A1 (en) Optimised set of oligonucleotides for bulk rna barcoding and sequencing
CN113073133A (zh) 放大微量dna并用于多种核酸检测的方法及核酸检测装置
HK40075118A (en) Methods for the multiplexed isothermal amplification of nucleic acid sequences
HK40075118B (en) Methods for the multiplexed isothermal amplification of nucleic acid sequences