CN115536450A - Environment-friendly novel liquid enzyme fertilizer taking Brazil ginseng stems and leaves as raw materials and preparation method thereof - Google Patents
Environment-friendly novel liquid enzyme fertilizer taking Brazil ginseng stems and leaves as raw materials and preparation method thereof Download PDFInfo
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- 239000003337 fertilizer Substances 0.000 title claims abstract description 61
- 239000007788 liquid Substances 0.000 title claims abstract description 51
- 239000002994 raw material Substances 0.000 title claims abstract description 45
- 235000008434 ginseng Nutrition 0.000 title claims abstract description 43
- 235000005035 Panax pseudoginseng ssp. pseudoginseng Nutrition 0.000 title claims abstract description 42
- 235000003140 Panax quinquefolius Nutrition 0.000 title claims abstract description 42
- 108090000790 Enzymes Proteins 0.000 title claims abstract description 15
- 102000004190 Enzymes Human genes 0.000 title claims abstract description 15
- 241000208340 Araliaceae Species 0.000 title claims abstract 10
- 238000002360 preparation method Methods 0.000 title claims description 16
- 241000894006 Bacteria Species 0.000 claims abstract description 57
- 239000002131 composite material Substances 0.000 claims abstract description 37
- XSQUKJJJFZCRTK-UHFFFAOYSA-N Urea Chemical compound NC(N)=O XSQUKJJJFZCRTK-UHFFFAOYSA-N 0.000 claims abstract description 20
- 239000004202 carbamide Substances 0.000 claims abstract description 20
- GSFSVEDCYBDIGW-UHFFFAOYSA-N 2-(1,3-benzothiazol-2-yl)-6-chlorophenol Chemical compound OC1=C(Cl)C=CC=C1C1=NC2=CC=CC=C2S1 GSFSVEDCYBDIGW-UHFFFAOYSA-N 0.000 claims abstract description 17
- 150000001413 amino acids Chemical class 0.000 claims abstract description 17
- YUKQRDCYNOVPGJ-UHFFFAOYSA-N thioacetamide Chemical compound CC(N)=S YUKQRDCYNOVPGJ-UHFFFAOYSA-N 0.000 claims abstract description 17
- DLFVBJFMPXGRIB-UHFFFAOYSA-N thioacetamide Natural products CC(N)=O DLFVBJFMPXGRIB-UHFFFAOYSA-N 0.000 claims abstract description 17
- CDMADVZSLOHIFP-UHFFFAOYSA-N disodium;3,7-dioxido-2,4,6,8,9-pentaoxa-1,3,5,7-tetraborabicyclo[3.3.1]nonane;decahydrate Chemical compound O.O.O.O.O.O.O.O.O.O.[Na+].[Na+].O1B([O-])OB2OB([O-])OB1O2 CDMADVZSLOHIFP-UHFFFAOYSA-N 0.000 claims abstract description 15
- 229910000402 monopotassium phosphate Inorganic materials 0.000 claims abstract description 15
- 235000019796 monopotassium phosphate Nutrition 0.000 claims abstract description 15
- GNSKLFRGEWLPPA-UHFFFAOYSA-M potassium dihydrogen phosphate Chemical compound [K+].OP(O)([O-])=O GNSKLFRGEWLPPA-UHFFFAOYSA-M 0.000 claims abstract description 15
- LWIHDJKSTIGBAC-UHFFFAOYSA-K potassium phosphate Substances [K+].[K+].[K+].[O-]P([O-])([O-])=O LWIHDJKSTIGBAC-UHFFFAOYSA-K 0.000 claims abstract description 15
- OTYBMLCTZGSZBG-UHFFFAOYSA-L potassium sulfate Chemical compound [K+].[K+].[O-]S([O-])(=O)=O OTYBMLCTZGSZBG-UHFFFAOYSA-L 0.000 claims abstract description 15
- 229910052939 potassium sulfate Inorganic materials 0.000 claims abstract description 15
- 235000011151 potassium sulphates Nutrition 0.000 claims abstract description 15
- 239000000706 filtrate Substances 0.000 claims description 24
- 238000002156 mixing Methods 0.000 claims description 22
- 238000003756 stirring Methods 0.000 claims description 20
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 18
- 241000223260 Trichoderma harzianum Species 0.000 claims description 16
- 241000193830 Bacillus <bacterium> Species 0.000 claims description 15
- 241000194108 Bacillus licheniformis Species 0.000 claims description 15
- 244000063299 Bacillus subtilis Species 0.000 claims description 15
- 235000014469 Bacillus subtilis Nutrition 0.000 claims description 15
- 238000001914 filtration Methods 0.000 claims description 12
- 239000000463 material Substances 0.000 claims description 10
- 235000013877 carbamide Nutrition 0.000 claims description 6
- 239000011259 mixed solution Substances 0.000 claims description 6
- 238000007789 sealing Methods 0.000 claims description 6
- 238000007873 sieving Methods 0.000 claims description 6
- 235000002791 Panax Nutrition 0.000 claims description 5
- 241000208343 Panax Species 0.000 claims description 5
- 238000000034 method Methods 0.000 claims description 5
- 235000015241 bacon Nutrition 0.000 claims description 4
- 238000000855 fermentation Methods 0.000 claims description 4
- 230000004151 fermentation Effects 0.000 claims description 4
- 238000010298 pulverizing process Methods 0.000 claims description 2
- 241000168720 Panax japonicus Species 0.000 claims 1
- 235000003174 Panax japonicus Nutrition 0.000 claims 1
- 241001268782 Paspalum dilatatum Species 0.000 claims 1
- 239000002689 soil Substances 0.000 abstract description 15
- 241000233866 Fungi Species 0.000 abstract description 4
- 201000010099 disease Diseases 0.000 abstract description 3
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 abstract description 3
- 241000196324 Embryophyta Species 0.000 abstract description 2
- 240000004371 Panax ginseng Species 0.000 description 33
- 241000219927 Eucalyptus Species 0.000 description 24
- 230000000052 comparative effect Effects 0.000 description 14
- 244000005700 microbiome Species 0.000 description 8
- IJGRMHOSHXDMSA-UHFFFAOYSA-N Atomic nitrogen Chemical compound N#N IJGRMHOSHXDMSA-UHFFFAOYSA-N 0.000 description 6
- 235000015097 nutrients Nutrition 0.000 description 6
- 230000009286 beneficial effect Effects 0.000 description 5
- 210000000481 breast Anatomy 0.000 description 5
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- 150000001875 compounds Chemical class 0.000 description 4
- 230000002195 synergetic effect Effects 0.000 description 4
- OAICVXFJPJFONN-UHFFFAOYSA-N Phosphorus Chemical compound [P] OAICVXFJPJFONN-UHFFFAOYSA-N 0.000 description 3
- 210000000038 chest Anatomy 0.000 description 3
- 230000000694 effects Effects 0.000 description 3
- 238000004519 manufacturing process Methods 0.000 description 3
- 229910052757 nitrogen Inorganic materials 0.000 description 3
- 229910052698 phosphorus Inorganic materials 0.000 description 3
- 239000011574 phosphorus Substances 0.000 description 3
- 239000000047 product Substances 0.000 description 3
- IOVCWXUNBOPUCH-UHFFFAOYSA-M Nitrite anion Chemical compound [O-]N=O IOVCWXUNBOPUCH-UHFFFAOYSA-M 0.000 description 2
- 230000000813 microbial effect Effects 0.000 description 2
- 239000000203 mixture Substances 0.000 description 2
- 239000003895 organic fertilizer Substances 0.000 description 2
- 238000012545 processing Methods 0.000 description 2
- 230000001737 promoting effect Effects 0.000 description 2
- 238000011160 research Methods 0.000 description 2
- 239000000126 substance Substances 0.000 description 2
- QJZYHAIUNVAGQP-UHFFFAOYSA-N 3-nitrobicyclo[2.2.1]hept-5-ene-2,3-dicarboxylic acid Chemical compound C1C2C=CC1C(C(=O)O)C2(C(O)=O)[N+]([O-])=O QJZYHAIUNVAGQP-UHFFFAOYSA-N 0.000 description 1
- 241000186361 Actinobacteria <class> Species 0.000 description 1
- 241000219317 Amaranthaceae Species 0.000 description 1
- 102000000634 Cytochrome c oxidase subunit IV Human genes 0.000 description 1
- 108050008072 Cytochrome c oxidase subunit IV Proteins 0.000 description 1
- 235000002789 Panax ginseng Nutrition 0.000 description 1
- ZLMJMSJWJFRBEC-UHFFFAOYSA-N Potassium Chemical compound [K] ZLMJMSJWJFRBEC-UHFFFAOYSA-N 0.000 description 1
- 238000010521 absorption reaction Methods 0.000 description 1
- XKMRRTOUMJRJIA-UHFFFAOYSA-N ammonia nh3 Chemical compound N.N XKMRRTOUMJRJIA-UHFFFAOYSA-N 0.000 description 1
- 238000004458 analytical method Methods 0.000 description 1
- 230000001093 anti-cancer Effects 0.000 description 1
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- 230000008859 change Effects 0.000 description 1
- 239000003795 chemical substances by application Substances 0.000 description 1
- 238000000354 decomposition reaction Methods 0.000 description 1
- 238000006731 degradation reaction Methods 0.000 description 1
- 238000011161 development Methods 0.000 description 1
- 230000018109 developmental process Effects 0.000 description 1
- MZGNSEAPZQGJRB-UHFFFAOYSA-N dimethyldithiocarbamic acid Chemical compound CN(C)C(S)=S MZGNSEAPZQGJRB-UHFFFAOYSA-N 0.000 description 1
- 238000001035 drying Methods 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 230000035558 fertility Effects 0.000 description 1
- 235000013305 food Nutrition 0.000 description 1
- 235000013402 health food Nutrition 0.000 description 1
- 239000004021 humic acid Substances 0.000 description 1
- 230000007062 hydrolysis Effects 0.000 description 1
- 238000006460 hydrolysis reaction Methods 0.000 description 1
- 238000009655 industrial fermentation Methods 0.000 description 1
- 230000002401 inhibitory effect Effects 0.000 description 1
- 230000004060 metabolic process Effects 0.000 description 1
- 244000000010 microbial pathogen Species 0.000 description 1
- 231100000252 nontoxic Toxicity 0.000 description 1
- 230000003000 nontoxic effect Effects 0.000 description 1
- 150000007524 organic acids Chemical class 0.000 description 1
- 239000005416 organic matter Substances 0.000 description 1
- 230000035699 permeability Effects 0.000 description 1
- 230000029553 photosynthesis Effects 0.000 description 1
- 238000010672 photosynthesis Methods 0.000 description 1
- 239000011591 potassium Substances 0.000 description 1
- 229910052700 potassium Inorganic materials 0.000 description 1
- 102000004169 proteins and genes Human genes 0.000 description 1
- 108090000623 proteins and genes Proteins 0.000 description 1
- 238000007670 refining Methods 0.000 description 1
- 230000029058 respiratory gaseous exchange Effects 0.000 description 1
- 230000010496 root system development Effects 0.000 description 1
- 239000002881 soil fertilizer Substances 0.000 description 1
- 238000006467 substitution reaction Methods 0.000 description 1
- 239000013589 supplement Substances 0.000 description 1
- 238000012360 testing method Methods 0.000 description 1
- 239000003053 toxin Substances 0.000 description 1
- 231100000765 toxin Toxicity 0.000 description 1
- 108700012359 toxins Proteins 0.000 description 1
- 235000013619 trace mineral Nutrition 0.000 description 1
- 239000011573 trace mineral Substances 0.000 description 1
- 230000001960 triggered effect Effects 0.000 description 1
Classifications
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- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05F—ORGANIC FERTILISERS NOT COVERED BY SUBCLASSES C05B, C05C, e.g. FERTILISERS FROM WASTE OR REFUSE
- C05F17/00—Preparation of fertilisers characterised by biological or biochemical treatment steps, e.g. composting or fermentation
- C05F17/20—Preparation of fertilisers characterised by biological or biochemical treatment steps, e.g. composting or fermentation using specific microorganisms or substances, e.g. enzymes, for activating or stimulating the treatment
-
- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05B—PHOSPHATIC FERTILISERS
- C05B7/00—Fertilisers based essentially on alkali or ammonium orthophosphates
-
- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05G—MIXTURES OF FERTILISERS COVERED INDIVIDUALLY BY DIFFERENT SUBCLASSES OF CLASS C05; MIXTURES OF ONE OR MORE FERTILISERS WITH MATERIALS NOT HAVING A SPECIFIC FERTILISING ACTIVITY, e.g. PESTICIDES, SOIL-CONDITIONERS, WETTING AGENTS; FERTILISERS CHARACTERISED BY THEIR FORM
- C05G3/00—Mixtures of one or more fertilisers with additives not having a specially fertilising activity
-
- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05G—MIXTURES OF FERTILISERS COVERED INDIVIDUALLY BY DIFFERENT SUBCLASSES OF CLASS C05; MIXTURES OF ONE OR MORE FERTILISERS WITH MATERIALS NOT HAVING A SPECIFIC FERTILISING ACTIVITY, e.g. PESTICIDES, SOIL-CONDITIONERS, WETTING AGENTS; FERTILISERS CHARACTERISED BY THEIR FORM
- C05G5/00—Fertilisers characterised by their form
- C05G5/20—Liquid fertilisers
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02W—CLIMATE CHANGE MITIGATION TECHNOLOGIES RELATED TO WASTEWATER TREATMENT OR WASTE MANAGEMENT
- Y02W30/00—Technologies for solid waste management
- Y02W30/40—Bio-organic fraction processing; Production of fertilisers from the organic fraction of waste or refuse
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- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Pest Control & Pesticides (AREA)
- Biotechnology (AREA)
- Biochemistry (AREA)
- Chemical Kinetics & Catalysis (AREA)
- General Chemical & Material Sciences (AREA)
- Microbiology (AREA)
- Molecular Biology (AREA)
- Engineering & Computer Science (AREA)
- Tropical Medicine & Parasitology (AREA)
- Fertilizers (AREA)
Abstract
The invention discloses an environment-friendly novel liquid ferment fertilizer taking Barcely ginseng stems and leaves as raw materials, which comprises the following raw materials in parts by weight: 5 to 10 parts of fresh Brazilian ginseng stem and leaf, 18 to 35 parts of urea, 10 to 25 parts of monopotassium phosphate, 5 to 15 parts of potassium sulfate, 0 to 4.5 parts of sodium borate decahydrate, 1 to 5 parts of brown sugar, 0 to 15 parts of water-soluble amino acid, 0.1 to 1.1 parts of composite growth-promoting bacteria, 0.3 to 1.5 parts of sodium dimethyldithiocarbamate and 0.2 to 0.9 part of thioacetamide. The novel environment-friendly liquid enzyme fertilizer can reduce the loss of effective components in the fertilizer, improve the utilization rate of the fertilizer, enhance the plant resistance, reduce diseases caused by bacteria, fungi and the like to the maximum extent, improve the soil microenvironment and improve the growth and quality of crops.
Description
Technical Field
The invention relates to the field of fertilizer processing, in particular to an environment-friendly novel liquid enzyme fertilizer taking stems and leaves of ginseng in Barceli as a raw material and a preparation method thereof.
Background
At present, the yield increase of agriculture generally depends on fertilizers, so that the environment pollution is caused on one hand, and the quality of agricultural products is influenced on the other hand. The "organic green barrier" has also become an important obstacle to export of agricultural products. Therefore, people gradually realize that while the fertilizer is used, the use proportion of the organic fertilizer must be increased, the organic matter content of soil must be increased, the original land fertility of the land must be continuously improved, and the organic fertilizer is used, so that the yield can be increased, the quality can be improved, and the ecological environment can be protected. With the national demand for ecological agriculture and sustainable agriculture, and the rise of global green food production and consumption, the development of green and nontoxic biological fertilizers becomes a hot tide.
The ferment fertilizer is prepared by adopting ferment fungus agricultural bioengineering technology, high-efficiency ferment fungus strains and an international advanced fungus fertilizer production process through modern industrial fermentation, processing, drying and refining on the basis of a reasonable formula. The ferment fertilizer contains rich organic matters, humic acid, amino acid and a large number of living bacteria, has strong fermentation and decomposition capacity, contains nitrogen, phosphorus and potassium nutrients necessary for the growth of crops and a plurality of medium and trace elements, and is added with a slow release agent in individual varieties to uniformly release the nutrients and prolong the fertilizer efficiency.
At present, most of the existing enzyme preparation methods are general preparation principles, and the produced enzyme fertilizer has limited promotion of crop growth.
The Brazilian ginseng Familia belongs to Amaranthaceae, is perennial herbaceous, stoloniferous, thick in rhizome, and is mainly distributed in original ecological regions of Brazilian goiya in tropical rain forest climatic zones of south America, 1994, and the Familia is approved as a health food by the American FDA, and is used for nourishing and treating various diseases. In the eighties of the last century, as Japanese scientists discovered the anti-cancer characteristic of Famifia, the research hot tide of scientists in various countries in the world on Famifia was triggered, and various single or compound products taking Brazilian ginseng as a raw material appeared. However, the Brazilian ginseng roots are mostly used at present, and the residual Brazilian ginseng stems and leaves after being picked are dried in the sun and then are directly burnt, so that not only is the resource wasted, but also the environment is polluted.
The stem and leaf dry matter of Brazilian ginseng contains 11.23 percent of total amount of 18 common amino acids and 13.9 percent of protein, and is a high-quality animal feed and fermentation raw material, which is detected by autonomous region analysis and test research center of Guangxi Zhuang nationality. Therefore, how to adopt Brazilian ginseng stems and leaves as raw materials, optimize the formula, develop a novel environment-friendly liquid ferment fertilizer which promotes the growth of crops and has low cost becomes a new research direction.
Disclosure of Invention
The invention aims to provide an environment-friendly novel liquid ferment fertilizer taking Brazilian ginseng stems and leaves as raw materials and a preparation method thereof, and aims to solve the practical technical problem of how to adopt Brazilian ginseng stems and leaves as raw materials, optimize a formula and develop the environment-friendly novel liquid ferment fertilizer which can promote the growth of crops and has low cost.
In order to solve the technical problems, the invention adopts the following technical scheme:
an environment-friendly novel liquid ferment fertilizer taking the stems and leaves of the ginseng in the Brazil as the raw material comprises the following raw materials in parts by weight: 5 to 10 parts of fresh Brazilian ginseng stem and leaf, 18 to 35 parts of urea, 10 to 25 parts of monopotassium phosphate, 5 to 15 parts of potassium sulfate, 0 to 4.5 parts of sodium borate decahydrate, 1 to 5 parts of brown sugar, 0 to 15 parts of water-soluble amino acid, 0.1 to 1.1 parts of composite growth-promoting bacteria, 0.3 to 1.5 parts of sodium dimethyldithiocarbamate and 0.2 to 0.9 part of thioacetamide.
Further, the environment-friendly novel liquid ferment fertilizer taking the stems and leaves of the panax papaveris as the raw material comprises the following raw materials in parts by weight: 8 parts of fresh Brazilian ginseng stem and leaf, 26 parts of urea, 18 parts of monopotassium phosphate, 12 parts of potassium sulfate, 2 parts of sodium borate decahydrate, 3 parts of brown sugar, 8 parts of water-soluble amino acid, 0.8 part of composite growth-promoting bacteria, 1 part of sodium dimethyldithiocarbamate and 0.7 part of thioacetamide.
Furthermore, the composite growth-promoting bacteria comprise one or more of EM bacteria, bacillus subtilis, bacillus licheniformis, bacillus nodosum and trichoderma harzianum.
Further, the composite growth-promoting bacteria comprise the following components in parts by weight: 0.1 to 0.5 portion of EM bacteria, 0 to 0.2 portion of bacillus subtilis, 0 to 0.1 portion of bacillus licheniformis, 0 to 0.1 portion of bacillus nodularis and 0 to 0.2 portion of trichoderma harzianum.
Further, the composite growth-promoting bacteria comprise the following components in parts by weight: 0.45 part of EM bacteria, 0.1 part of bacillus subtilis, 0.06 part of bacillus licheniformis, 0.05 part of bacillus nodosum and 0.14 part of trichoderma harzianum.
The invention also provides a preparation method of the environment-friendly novel liquid ferment fertilizer taking the stems and leaves of the panax ginseng in the bacon as a raw material, which comprises the following steps:
s1: preparing composite growth-promoting bacteria for later use;
s2: pulverizing fresh Brazilian ginseng stem and leaf, sieving, mixing the obtained pulverized material with brown sugar and water, sealing, fermenting, and filtering to obtain filtrate a;
s3: uniformly mixing urea, monopotassium phosphate, potassium sulfate, sodium borate decahydrate and water-soluble amino acid according to parts by weight, dissolving in water, and filtering to obtain filtrate b;
s4: mixing the composite growth-promoting bacteria prepared in the step S1, the filtrate a prepared in the step S2 and the filtrate b prepared in the step S3, and then stirring to prepare a mixed solution;
s5: and (5) adding water into the mixed liquid obtained in the step (S4), and stirring to obtain the environment-friendly novel liquid enzyme fertilizer.
Furthermore, the mesh number of the screen in the step S2 is 100-150 meshes.
Further, the time for sealed fermentation in step S2 is 2.8-3.3 months.
Further, the conditions for stirring in step S4 are: stirring for 10-15 min at the rotating speed of 200-300 r/min.
Further, the stirring condition in step S5 is to stir for 7 to 10min at a rotation speed of 300 to 400 r/min.
The invention has the following beneficial effects:
(1) Under the mutual cooperation of the compound growth-promoting bacteria, the sodium dimethyldithiocarbamate and the thioacetamide, the compound growth-promoting bacteria, the dimethyl dithiocarbamate and the thioacetamide have a synergistic effect, provide a large number of advantageous microbial community, supplement nutrient components in soil for planting eucalyptus, secrete nutrient substances for the eucalyptus to absorb, promote the growth of the eucalyptus and improve the average tree height and the average chest diameter of the eucalyptus.
(2) After 1-year-old eucalyptus is planted by the environment-friendly novel liquid ferment fertilizer for 2 years, the average tree height of the eucalyptus is more than 13.3m, and the breast diameter is more than 10.4 cm.
(3) The environment-friendly novel liquid ferment fertilizer taking the Brazilian ginseng stems and leaves as the raw material can realize automatic degradation, is environment-friendly, does not pollute the environment, and can greatly reduce the production cost because a large amount of Brazilian ginseng stems and leaves are utilized as the raw material.
Detailed Description
In order that the invention may be better understood, reference will now be made in detail to the present invention as illustrated in the accompanying drawings. It should be emphasized that the following description is merely exemplary in nature and is not intended to limit the scope of the invention or its application.
In the embodiment, the environment-friendly novel liquid ferment fertilizer taking the stems and leaves of the Brazilian ginseng as the raw material comprises the following raw materials in parts by weight: 5 to 10 parts of fresh Brazilian ginseng stem leaves, 18 to 35 parts of urea, 10 to 25 parts of monopotassium phosphate, 5 to 15 parts of potassium sulfate, 0 to 4.5 parts of sodium borate decahydrate, 1 to 5 parts of brown sugar, 0 to 15 parts of water-soluble amino acid, 0.1 to 1.1 parts of composite growth-promoting bacteria, 0.3 to 1.5 parts of sodium dimethyldithiocarbamate and 0.2 to 0.9 part of thioacetamide;
the composite growth-promoting bacterium comprises the following components in parts by weight: 0.1 to 0.5 portion of EM bacteria, 0 to 0.2 portion of bacillus subtilis, 0 to 0.1 portion of bacillus licheniformis, 0 to 0.1 portion of bacillus tubercle and 0 to 0.2 portion of trichoderma harzianum;
the preparation method of the environment-friendly novel liquid ferment fertilizer taking the stems and leaves of Brazilian ginseng as the raw material comprises the following steps:
s1: uniformly mixing EM (effective microorganisms), bacillus subtilis, bacillus licheniformis, bacillus tubercle and trichoderma harzianum according to parts by weight to prepare composite growth-promoting bacteria;
s2: crushing fresh Brazilian ginseng stems and leaves, sieving the crushed material by a sieve of 100 to 150 meshes, uniformly mixing the obtained crushed material with brown sugar and 6 to 20 parts of water, sealing and fermenting the mixture for 2.8 to 3.3 months, and filtering the mixture to obtain filtrate a;
s3: uniformly mixing urea, monopotassium phosphate, potassium sulfate, sodium borate decahydrate and water-soluble amino acid according to parts by weight, dissolving in 35-100 parts of water, and filtering to obtain filtrate b;
s4: mixing the composite growth-promoting bacteria prepared in the step S1, the filtrate a prepared in the step S2 and the filtrate b prepared in the step S3, and then stirring for 10-15 min at the rotating speed of 200-300 r/min to prepare a mixed solution;
s5: and (4) adding 300-450 parts of water into the mixed liquid obtained in the step (S4), and stirring at the rotating speed of 300-400 r/min for 7-10 min to obtain the novel environment-friendly liquid enzyme fertilizer.
The following examples are given by way of illustration.
Example 1
An environment-friendly novel liquid ferment fertilizer taking the stems and leaves of the ginseng in the Brazil as the raw material comprises the following raw materials in parts by weight: 5 parts of fresh Brazilian ginseng stem and leaf, 20 parts of urea, 11 parts of monopotassium phosphate, 5 parts of potassium sulfate, 1 part of sodium borate decahydrate, 1.2 parts of brown sugar, 2 parts of water-soluble amino acid, 0.2 part of composite growth-promoting bacteria, 0.4 part of sodium dimethyldithiocarbamate and 0.3 part of thioacetamide;
the composite growth-promoting bacterium comprises the following components in parts by weight: 0.1 part of EM bacteria, 0.03 part of bacillus subtilis, 0.02 part of bacillus licheniformis, 0.03 part of bacillus nodosum and 0.02 part of trichoderma harzianum;
the preparation method of the environment-friendly novel liquid ferment fertilizer taking the stems and leaves of Brazilian ginseng as the raw material comprises the following steps:
s1: uniformly mixing EM (effective microorganisms), bacillus subtilis, bacillus licheniformis, bacillus tubercle and trichoderma harzianum according to parts by weight to prepare composite growth-promoting bacteria;
s2: crushing fresh Brazilian ginseng stems and leaves, sieving the crushed material by a 100-mesh sieve, uniformly mixing the obtained crushed material with brown sugar and 7 parts of water, sealing and fermenting for 2.8 months, and filtering to obtain filtrate a;
s3: uniformly mixing urea, monopotassium phosphate, potassium sulfate, sodium borate decahydrate and water-soluble amino acid according to parts by weight, dissolving in 38 parts of water, and filtering to obtain filtrate b;
s4: mixing the composite growth-promoting bacteria prepared in the step S1, the filtrate a prepared in the step S2 and the filtrate b prepared in the step S3, and then stirring for 15min at the rotating speed of 200r/min to prepare a mixed solution;
s5: and (4) adding 320 parts of water into the mixed liquid obtained in the step (S4), and stirring at the rotating speed of 300r/min for 10min to obtain the novel environment-friendly liquid enzyme fertilizer.
Example 2
An environment-friendly novel liquid ferment fertilizer taking stems and leaves of radix ginseng in the western area as raw materials comprises the following raw materials in parts by weight: 8 parts of fresh Brazilian ginseng stem and leaf, 26 parts of urea, 18 parts of monopotassium phosphate, 12 parts of potassium sulfate, 2 parts of sodium borate decahydrate, 3 parts of brown sugar, 8 parts of water-soluble amino acid, 0.8 part of composite growth-promoting bacteria, 1 part of sodium dimethyldithiocarbamate and 0.7 part of thioacetamide;
the composite growth-promoting bacterium comprises the following components in parts by weight: 0.45 part of EM bacteria, 0.1 part of bacillus subtilis, 0.06 part of bacillus licheniformis, 0.05 part of bacillus nodosum and 0.14 part of trichoderma harzianum;
the preparation method of the environment-friendly novel liquid ferment fertilizer taking the stems and leaves of Brazilian ginseng as the raw material comprises the following steps:
s1: uniformly mixing EM (effective microorganisms), bacillus subtilis, bacillus licheniformis, bacillus tubercle and trichoderma harzianum according to parts by weight to prepare composite growth-promoting bacteria;
s2: crushing fresh Brazilian ginseng stems and leaves, sieving the crushed material with a 120-mesh sieve, uniformly mixing the obtained crushed material with brown sugar and 15 parts of water, sealing and fermenting for 3 months, and filtering to obtain filtrate a;
s3: uniformly mixing urea, monopotassium phosphate, potassium sulfate, sodium borate decahydrate and water-soluble amino acid according to parts by weight, dissolving in 70 parts of water, and filtering to obtain filtrate b;
s4: mixing the composite growth-promoting bacteria prepared in the step S1, the filtrate a prepared in the step S2 and the filtrate b prepared in the step S3, and then stirring for 10min at the rotating speed of 300r/min to prepare a mixed solution;
s5: and (4) adding 380 parts of water into the mixed liquid obtained in the step (S4), and stirring at the rotating speed of 400r/min for 7min to obtain the novel environment-friendly liquid enzyme fertilizer.
Example 3
An environment-friendly novel liquid ferment fertilizer taking the stems and leaves of the ginseng in the Brazil as the raw material comprises the following raw materials in parts by weight: 9 parts of fresh Brazilian ginseng stem and leaf, 32 parts of urea, 20 parts of monopotassium phosphate, 13 parts of potassium sulfate, 4 parts of sodium borate decahydrate, 5 parts of brown sugar, 14 parts of water-soluble amino acid, 1 part of composite growth-promoting bacteria, 1.5 parts of sodium dimethyldithiocarbamate and 0.8 part of thioacetamide;
the composite growth-promoting bacterium comprises the following components in parts by weight: 0.5 part of EM bacteria, 0.14 part of bacillus subtilis, 0.1 part of bacillus licheniformis, 0.1 part of bacillus tubercle and 0.16 part of trichoderma harzianum;
the preparation method of the environment-friendly novel liquid ferment fertilizer taking the stems and leaves of Brazilian ginseng as the raw material comprises the following steps:
s1: uniformly mixing EM (effective microorganisms), bacillus subtilis, bacillus licheniformis, bacillus tubercle and trichoderma harzianum according to parts by weight to prepare composite growth-promoting bacteria;
s2: crushing fresh Brazilian ginseng stems and leaves, sieving the crushed material with a 150-mesh sieve, uniformly mixing the obtained crushed material with brown sugar and 19 parts of water, sealing and fermenting for 3.2 months, and filtering to obtain filtrate a;
s3: uniformly mixing urea, monopotassium phosphate, potassium sulfate, sodium borate decahydrate and water-soluble amino acid according to parts by weight, dissolving in 90 parts of water, and filtering to obtain filtrate b;
s4: mixing the composite growth-promoting bacteria prepared in the step S1, the filtrate a prepared in the step S2 and the filtrate b prepared in the step S3, and then stirring for 15min at the rotating speed of 200r/min to prepare a mixed solution;
s5: and (4) adding 420 parts of water into the mixed liquid obtained in the step (S4), and stirring at the rotating speed of 300r/min for 10min to obtain the novel environment-friendly liquid enzyme fertilizer.
Comparative example 1
The preparation process is basically the same as that of example 2, except that the raw materials for preparing the environment-friendly novel liquid ferment fertilizer lack the composite growth-promoting bacteria, the sodium dimethyldithiocarbamate and the thioacetamide.
Comparative example 2
The preparation process is basically the same as that of comparative example 1, except that the raw materials for preparing the environment-friendly novel liquid ferment fertilizer are added with the composite growth-promoting bacteria.
Comparative example 3
The preparation process is basically the same as that of comparative example 1, except that sodium dimethyldithiocarbamate is added into the raw materials for preparing the environment-friendly novel liquid ferment fertilizer.
Comparative example 4
The preparation process is basically the same as that of comparative example 1, except that thioacetamide is added into the raw materials for preparing the environment-friendly novel liquid ferment fertilizer.
The liquid ferment fertilizers prepared in examples 1 to 3 and comparative examples 1 to 4 were tested for fertilizer efficiency by the following method:
selecting 1 year old eucalyptus which is strong in tree age and basically identical in growth vigor in a test sample plot, selecting 7 samples in a eucalyptus base, wherein each sample is 1 mu, planting 111 eucalyptus plants per mu, using the liquid enzyme fertilizers prepared in the examples 1-3 and the comparative examples 1-4 for the 7 samples respectively, measuring the fertilizer application amount of each eucalyptus plant in the 1 st year, the fertilizer application amount of each eucalyptus plant in the 2 nd year, measuring the tree height and the breast diameter of each eucalyptus sample after planting for 2 years, and calculating the average tree height and the breast diameter of each eucalyptus sample respectively, wherein the results are shown in the following table.
| Experimental group | Average height of tree (m) | Mean chest diameter (cm) |
| Example 1 | 13.3 | 10.4 |
| Example 2 | 13.9 | 11.0 |
| Example 3 | 13.5 | 10.8 |
| Comparative example 1 | 10.1 | 7.6 |
| Comparative example 2 | 11.5 | 8.9 |
| Comparative example 3 | 10.6 | 8.2 |
| Comparative example 4 | 11.1 | 8.6 |
From the above table, it can be seen that: (1) As can be seen from the data calculation of the example 2 and the comparative examples 1 to 4, the composite growth-promoting bacteria, the sodium dimethyldithiocarbamate and the thioacetamide play a synergistic role in preparing the novel environment-friendly liquid ferment fertilizer, and the average tree height and the average chest diameter of the eucalyptus are synergistically improved. This may be because: the EM bacteria in the composite growth-promoting bacteria can quickly and stably occupy the ecological status in the soil due to the easy growth and reproduction in the soil, and form a beneficial dominant community of microorganisms, thereby controlling the reproduction of pathogenic microorganisms and the attack on crops; beneficial microbial bacteria are formed to perform symbiotic co-reproduction of actinomycetes and the like in soil, a good crop growth environment is formed, soil fertilizer is improved, and soil property is improved; in addition, EM bacteria can enhance plant metabolism, promote photosynthesis, strengthen leaf protecting film, resist pathogenic bacteria and promote root system development. The bacillus subtilis, the bacillus licheniformis and the bacillus nodosum in the composite growth-promoting bacteria can secrete a large amount of organic acid, dissolve inorganic phosphorus and improve the absorption and utilization of eucalyptus on phosphorus. The trichoderma harzianum in the composite growth-promoting bacteria can eliminate soil disease toxins and harmful microorganisms, and increase the content of organic matters and microorganisms; in addition, the trichoderma harzianum can activate soil, promote the granular structure of the soil, increase air permeability, eliminate hardening, adjust the pH value of the soil and restore the original power of the soil. The urea can be absorbed and utilized by eucalyptus after being hydrolyzed into ammonium nitrogen by urease, the sodium dimethyldithiocarbamate can compete with the urea for a binding site with the urease, and the affinity of the sodium dimethyldithiocarbamate with the urease is higher, so that the urea reduces the probability of acting with the urease, the aim of inhibiting the urea hydrolysis is fulfilled, and the effect of slowly releasing nitrogen elements is achieved. The thioacetamide can influence the respiration of nitrosobacteria and the activity of cytochrome oxidase, change the microenvironment of soil, reduce the pH value of the soil, make the nitrite bacteria difficult to breathe and further inhibit the reproduction of the nitrite bacteria. The sodium dimethyldithiocarbamate and thioacetamide can improve the fertilizer efficiency of the urea under the synergistic action, thereby reducing the loss of nitrogen element and promoting the growth of eucalyptus. Under the mutual matching of the compound growth-promoting bacteria, the sodium dimethyldithiocarbamate and the thioacetamide, a synergistic effect is achieved, a large number of advantageous communities of beneficial microorganisms are provided, nutrient components in soil for planting eucalyptus are supplemented, nutrient substances are secreted to be absorbed by the eucalyptus, the growth of the eucalyptus is promoted, and the average tree height and the average breast diameter of the eucalyptus are improved.
(2) As can be seen from the data of the embodiments 1 to 3, the average tree height of the eucalyptus trees is more than 13.3m and the diameter at breast height is more than 10.4cm after the 1-year eucalyptus is planted for 2 years, which indicates that the environment-friendly novel liquid ferment fertilizer prepared by the invention has good fertilizer effect and is beneficial to promoting the rapid growth of the eucalyptus.
The above description should not be taken as limiting the invention to the embodiments, but rather, as will be apparent to those skilled in the art to which the invention pertains, numerous simplifications or substitutions may be made without departing from the spirit of the invention, which shall be deemed to fall within the scope of the invention as defined by the claims appended hereto.
Claims (10)
1. The environment-friendly novel liquid ferment fertilizer taking the stems and leaves of the panax papaveris as the raw material is characterized by comprising the following raw materials in parts by weight: 5 to 10 parts of fresh Brazilian ginseng stem and leaf, 18 to 35 parts of urea, 10 to 25 parts of monopotassium phosphate, 5 to 15 parts of potassium sulfate, 0 to 4.5 parts of sodium borate decahydrate, 1 to 5 parts of brown sugar, 0 to 15 parts of water-soluble amino acid, 0.1 to 1.1 parts of composite growth-promoting bacteria, 0.3 to 1.5 parts of sodium dimethyldithiocarbamate and 0.2 to 0.9 part of thioacetamide.
2. The environment-friendly novel liquid ferment fertilizer taking the stems and leaves of the panax papaveris as the raw material as the claim 1, is characterized by comprising the following raw materials in parts by weight: 8 parts of fresh Brazilian ginseng stem and leaf, 26 parts of urea, 18 parts of monopotassium phosphate, 12 parts of potassium sulfate, 2 parts of sodium borate decahydrate, 3 parts of brown sugar, 8 parts of water-soluble amino acid, 0.8 part of composite growth-promoting bacteria, 1 part of sodium dimethyldithiocarbamate and 0.7 part of thioacetamide.
3. The environment-friendly novel liquid ferment fertilizer taking the stems and leaves of paspalum japonicum as raw materials according to claim 1 or 2, characterized in that the composite growth-promoting bacteria comprise one or more of EM bacteria, bacillus subtilis, bacillus licheniformis, bacillus nodularis and trichoderma harzianum.
4. The novel environment-friendly liquid ferment fertilizer taking the stems and leaves of the panax papaveris as the raw material as claimed in claim 3, wherein the composite growth-promoting bacteria comprise the following components in parts by weight: 0.1 to 0.5 portion of EM bacteria, 0 to 0.2 portion of bacillus subtilis, 0 to 0.1 portion of bacillus licheniformis, 0 to 0.1 portion of bacillus nodularis and 0 to 0.2 portion of trichoderma harzianum.
5. The novel environment-friendly liquid ferment fertilizer taking the stems and leaves of the panax papaveris as the raw material as claimed in claim 4, wherein the composite growth-promoting bacteria comprise the following components in parts by weight: 0.45 part of EM bacteria, 0.1 part of bacillus subtilis, 0.06 part of bacillus licheniformis, 0.05 part of bacillus nodosum and 0.14 part of trichoderma harzianum.
6. The preparation method of the novel environment-friendly liquid ferment fertilizer taking the stems and leaves of the panax japonicus as the raw material according to any one of claims 1 to 5, is characterized by comprising the following steps:
s1: preparing composite growth-promoting bacteria for later use;
s2: pulverizing fresh Brazilian ginseng stem and leaf, sieving, mixing the obtained pulverized material with brown sugar and water, sealing, fermenting, and filtering to obtain filtrate a;
s3: uniformly mixing urea, monopotassium phosphate, potassium sulfate, sodium borate decahydrate and water-soluble amino acid according to parts by weight, dissolving in water, and filtering to obtain filtrate b;
s4: mixing the composite growth-promoting bacteria prepared in the step S1, the filtrate a prepared in the step S2 and the filtrate b prepared in the step S3, and then stirring to prepare a mixed solution;
s5: and (5) adding water into the mixed liquid obtained in the step (S4), and stirring to obtain the environment-friendly novel liquid ferment fertilizer.
7. The method for preparing the novel environment-friendly liquid enzyme fertilizer with the stems and leaves of the bacon ginseng as the raw material as the claim 6, wherein the mesh number of the sieve in the step S2 is 100-150 meshes.
8. The method for preparing the novel environment-friendly liquid enzyme fertilizer with the stems and leaves of the bacon ginseng as the raw material as the claim 6, wherein the time for the sealed fermentation in the step S2 is 2.8-3.3 months.
9. The method for preparing the environment-friendly novel liquid ferment fertilizer by taking the stems and leaves of the radix ginseng basketii as the raw material as claimed in claim 6, wherein the stirring conditions in the step S4 are as follows: stirring for 10-15 min at the rotating speed of 200-300 r/min.
10. The method for preparing the novel environment-friendly liquid enzyme fertilizer with the stems and leaves of the bacon ginseng as the raw material as the claim 6, wherein the stirring condition in the step S5 is that the stirring is carried out for 7-10 min at the rotating speed of 300-400 r/min.
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