JP2000262277A - Microbial preparation - Google Patents

Abstract

PROBLEM TO BE SOLVED: To obtain a microbial preparation useful for making decomposable waste materials, feces of domestic animals, etc., as a completely matured com post by mixing an enzyme for decomposing proteins, cellulose, starch, lipids, etc., with a specific Bacillus subtilis-related bacteria having no hemolytic prop erty. SOLUTION: This microbial preparation used for an acceleration of the fermentation of decomposable waste materials or excreted materials, is produced by mixing or adding one kind or two or more kinds enzymes capable of decomposing proteins, cellulose, starch, lipids, etc., with a microorganisms of Bacillus subtilis-related bacteria not having hemolytic activity, having a larger cellular volume as compared with that of a standard Bacillus subtilis (IFO-3134 strain), and since it shows almost no inducing period and propagates in a short period of time in a nutrient broth shaking culture at 37 deg.C, generating an extremely larger metabolic energy by its propagation with a synergistic effect of the above effects as compared with that of the above standard Bacillus subtilis. Further, as the microorganism, Bacillus sp OYK-01-600 (FERM BP-6394), etc., are used.

Description

DETAILED DESCRIPTION OF THE INVENTION

[0001]

BACKGROUND OF THE INVENTION 1. Field of the Invention The present invention relates to a cell preparation which is mixed or added at an early stage in the decomposition process of putrefactive waste or excrement to promote decomposition.

[0002]

2. Description of the Related Art The use of useful microorganisms to improve the environment and the development of drugs useful for humans have been practiced for a long time, and many commercialized products have been put on the market. Above all, subtilis of the genus Bacillus is widely distributed in nature and has an activity in decomposing organic substances, so that it has high utility as a human-friendly bacterium. On the other hand, related bacteria of Bacillus cereus are widely distributed in soil and form insecticidal crystal proteins at the time of buds, so they are used as insecticidal microbial pesticides. The production of antibiotics using Bacillus pumilus is described in JP-A-50-69295, and a number of techniques for producing novel antibiotics from bacterial products of the genus Bacillus have been proposed. In addition, as a deodorant,
No. 146556 discloses a mixed composition of rice bran, bran, butyric acid bacteria, and Bacillus subtilis, and is capable of sustained deodorization. As described above, among the genus Bacillus, a large number of bacteria having different characteristics depending on the use are being used.

[0003] In addition, as the population increases, various types of putrefactive wastes and excreta are produced in large quantities, and pollution of the environment is taken up as a serious problem, and many methods for effectively utilizing microorganisms have been proposed. Where you are. One of the techniques for shortening the maturation period of composting or for reducing odor emission to mature is described in a large number of prior arts. Among them, Japanese Patent Application Laid-Open No. 1-215886 discloses that useful microorganisms are porous. After contact with natural coconut fiber to absorb moisture, fermentation treatment is performed by adding necessary nutrients, and microorganisms are sufficiently propagated in pores inside coconut fiber to obtain an excellent soil conditioner. Is described. As useful microorganisms, anaerobic bacteria such as Lactobacillus and Micrococcus and aerobic bacteria such as Bacillus and Saccharomyces are described. JP-A-3-159981 discloses that a layer of chicken manure with a controlled water content, a fermenting agent containing a facultative aerobic or anaerobic bacterium of the genus Bacillus sublis and a fine powder of a mineral containing a rare earth element are mixed. A method for producing a mature fermented compost in which layers are alternately stacked and fermented is described. Also, in Japanese Patent Application Laid-Open No. 8-26873, putrefactive waste is produced under a poor oxygen condition by generating frictional heat by a mechanical means such as a screw and ventilated to 40 to 70 ° C., thereby causing odor caused by facultative anaerobic bacteria. After suppressing or killing the microorganisms including the substance-producing bacteria, fermentation by the Galus X bacteria that survived under the high-temperature and poor oxygen environment under aerobic conditions while maintaining the temperature of these wastes at a high temperature. It is described that a composting material is obtained by starting the fermentation with priority. The Galus X bacterium mentioned here is a new species of the genus Bacillus and is considered to be an aerobic thermophilic bacterium.

As described above, there are many bacteria useful for maturation of compost in the genus Bacillus, but the means for utilizing the characteristics of each of the employed bacteria are different, and the process of action is also different. In general, the most useful target for microorganisms such as fungi is that they are in a eutrophic environment.The reason for this is that the microorganism density required for the microorganism to exert its full effect is sufficient. Is trying to secure. Therefore, during the period before the microorganisms are actually administered, the nutrients necessary for survival are not initially provided, but it is difficult to say that they provide an environment that allows them to grow. is there. According to the present inventors' large achievements, in the composting of livestock dung, the moisture content of dung such as livestock is 50 to 60%, and the temperature of the environment is 25%.
At ~ 35 ° C, nutrients contained in feces are ingested, bacteria contained in feces are actively activated and fermentation proceeds, but fermentation does not proceed under low moisture conditions such as winter due to high moisture content. Have experienced that. It is also effective to supply eutrophic sources that are easy to ingest microbes contained in feces, but existing microorganisms have a slow growth rate, and eutrophic sources (proteins, etc.) themselves are added to the cost of compost. This puts a heavy burden on composters. An object of the present invention is to provide an enzyme or a nitrogen source for shaping a nutrient source of a composting target into an easily ingestible form when a specific bacterium proliferates, and further impart an oxygen supply source to the bacterium. The purpose of the present invention is to provide a method and means for obtaining a safe ripe compost irrespective of the season, while suppressing the growth of other undesirable bacteria by growing and maintaining the high density. The OYK bacterium, which is a novel microorganism described in the claims, is a bacterium belonging to the genus Bacillus and is related to subtilis, and the details thereof are described in Japanese Patent Application No. 9-237974. The explanation is omitted here.

[0005]

[Problems to be solved by the present invention] An object of the present invention is to disassemble feces such as livestock as safe ripened compost regardless of the season as an example of putrefactive waste or excrement. And to provide a cell preparation that promotes fermentation.

[0006]

DISCLOSURE OF THE INVENTION The present invention relates to a Bacillus tin butyls-related bacterium having no hemolytic properties, and comprising a standard Bacillus subtilis (IFO).
-3134 strain), the cell volume is larger, and 37
In nutrient broth shaking culture at ℃, there is almost no induction period in the initial stage of culture, and the growth is short, and due to these synergistic effects, the metabolizable energy generated by the growth is extremely large as compared with that of the standard B. subtilis. Mixing or adding one or more enzymes that decompose proteins, cellulose, starch, lipids, etc. to microorganisms characterized in that they reduce the molecular weight of various organic nutrients contained in feces such as livestock and make them easier to ingest This obviates the need for additional eutrophic sources. Examples of the enzyme include protease, cellulase, amylase, lipase and the like. Proteases are effective for decomposing proteins, amylase is effective for decomposing starch, cellulase is effective for decomposing fiber, and lipase is effective for decomposing lipid. Also, when adding or mixing the enzyme, an appropriate amount of defatted soybean granules or fine powder is added, and if an easily ingestible plant protein source is supplied, the effect of promoting the growth of the microorganism even when the environment is low in winter or the like is low. There is.

[0007] To explain the above-mentioned microorganism in more detail, it is a non-haemolytic bacillus tin butyls-related bacterium having a cell volume four times or more that of a standard Bacillus subtilis (IFO-3134 strain). In addition, in the nutrient broth shaking culture at 37 ° C., since there is almost no induction period in the initial stage of culture, the initial number of cells at 10 ³ cells / ml is 10 ⁷ cells / ml in about 4 hours.
The metabolic energy generated by the proliferation is 10,000 times or more as compared with that of the standard Bacillus subtilis.

[0008] Instead of natural soybean protein, at least one or more carbon compounds, nitrogen-containing compounds, phosphorus-containing compounds, and sulfur-containing compounds are added or added to the microorganisms as a eutrophic source. When culturing microorganisms, there are cases where they are added to solids such as soybeans, and cases where they are carried out in a liquid such as nutrient broth shaking culture.In the former case, powdering is carried out including soybeans. Since it can be used, the eutrophic source is soy protein, and it is possible to supply carbon, nitrogen, phosphorus, and other elements necessary for the growth of microorganisms in a well-balanced manner. However, in order to absorb these nutrients, the microorganisms need to secrete the degrading enzyme, which takes time. On the other hand, in the latter case of liquid culture, the microorganisms are extracted by filtration, so that a small amount of protein source is contained, but nutrient sources for growth are insufficient. Therefore, it is necessary to supply main nutrients such as carbon, nitrogen, phosphorus and sulfur to make up for the shortfall. As described above, soy protein may be used, but it is advisable to use a low-molecular compound that is more easily absorbed by microorganisms. Such compounds include the following. As the carbon compound, aromatic or aliphatic hydrocarbon, alcohol, glycol,
Monohydric or polyhydric alcohols such as glycerin, aldehydes, ketones, fats and oils and the like can be mentioned. In addition, organic acids or their derivatives, such as esters, glucose, starch, and carbohydrates such as cellulose can be exemplified. Furthermore, examples of the compound containing carbon and nitrogen elements include amino acids, oligopeptides, proteins, and ethanolamine. As the nitrogen-containing compound, compounds generally put to practical use as nitrogenous fertilizers, for example, ammonium chloride, ammonium sulfate, ammonium nitrate, ureaform, isobutylidene diurea, ureaset, crotonidene diurea, glycoluril, guanylurea, oxamide and the like Urea derivatives, nitrates such as sodium nitrate and potassium nitrate can be used. In addition, for example, organic acid ammonium salts such as ammonium citrate and ammonium tartrate can also be used. Of these, urea and urea derivatives are particularly preferred. Urea and urea derivatives may be used alone, or a processed product whose surface is coated with fats and oils, wax, sulfur or the like to control the elution amount may be used. Further, if necessary, a nitrification inhibitor such as thiourea or a derivative thereof, dicyandiamide and the like may be added. Examples of the phosphorus-containing compound include phosphates such as phosphoric acid or sodium phosphate, calcium phosphate, and ammonium phosphate, as well as conjugated phosphoric acid or sodium tripolyphosphate, condensed phosphates such as sodium pyrophosphate, and further, carbon, Phosphoric acid esters such as nucleosides and the like can be exemplified as the compound containing a phosphorus element simultaneously with nitrogen. Examples of the sulfur-containing compound include sulfates such as ammonium sulfate, organic sulfonic acids or salts thereof, sulfamic acids or salts thereof, and compounds containing a sulfur element simultaneously with carbon and nitrogen, such as thiourea, thioacetamide, and cysteine and methionine. Sulfur-containing amino acids and the like can be exemplified.

Further, the above-mentioned carbon compound, nitrogen-containing compound,
A phosphorus-containing compound or a sulfur-containing compound is used after being subjected to slow-acting or slow-acting processing. Among these compounds, for example, ureaform, isobutylinidene diurea, ureaset, oxamide and the like, there are compounds having a slow-acting property that elutes slowly in water, but a compound having an immediate action has a slow-acting property. Alternatively, it can be processed into a slow-release, slow release or release with controlled release rate or dissolution rate to prevent rapid runoff or runoff from the fermentation system and maintain an effective concentration in the system. Can be used. As the mixture, for example, a powder-granular mixture obtained by granulating and drying these compounds and a polymer compound in a solution or a dispersion mixture using a spray dryer or the like can be used.
Such mixtures generally exhibit a slow release. In addition, these compounds in powder or granular form are coated with a polymer compound,
Mixtures having a controlled release or dissolution rate by coating or encapsulation can be used. The coating or coating can be performed using various commonly used granulation coating apparatuses, for example, a fluidized bed coating machine and a pan coating machine. As the high molecular compound, soluble high molecular compounds such as starch, gelatin, polyvinyl alcohol and carboxymethyl cellulose, decomposable high molecular compounds such as polylactic acid, waxes, fats and oils, and sulfur can be used. Such mixtures generally exhibit a delayed action.
In addition, among the compounds serving as the above-mentioned eutrophic source, a liquid compound is used by impregnating pulp, cellulose or the like as a medium.

In order to supplement the supply of oxygen by ventilation, an oxygen-generating compound is added as an oxygen supply source. Examples of the oxygen generating compound include sodium peroxide carbonate, calcium peroxide and the like. For aerobic and facultatively anaerobic microorganisms, an air-permeable environment is preferred, so coffee scum (CFP) is often used as a porous material, but it takes more time and energy to decompose fibrous materials. This can be omitted or the amount used can be kept small. Sodium peroxide carbonate supplies oxygen and heat energy to improve the environment in which the above-mentioned novel microorganisms grow.

The above-mentioned oxygen-generating compound is used after being subjected to slow-acting or slow-acting processing. If these peroxides are granulated, coated with a water-soluble or alkali-water-soluble film and mixed or added, the elution time can be adjusted, so that frequent switching operations can be reduced. In order to adjust such elution time, the above-mentioned slow-acting or slow-acting processing means is similarly used. In addition, since the elution time can be controlled by the wax coating in conjunction with the rise in temperature, it is possible to cope with variations in the fermentation time under various different environments. This technique can also be applied to compounds intended to supply a nutrient source such as the carbon compounds and nitrogen-containing compounds described above.

The above-mentioned carbon compounds, nitrogen-containing compounds, phosphorus-containing compounds, sulfur-containing compounds, and oxygen-generating compounds may be added or mixed together with microorganisms and the like at an early stage, but are added by spraying during the fermentation process. This is also included in the present invention.
Then, in order to simplify the operation as much as possible, if an immediate-acting compound and a compound that has been subjected to slow-acting or slow-acting processing are simultaneously added or mixed at the initial stage, an appropriate amount of nutrient source and Oxygen can be provided to microorganisms.

The microorganisms include the novel microorganisms described below. Bacillus sp. OYK-01-6
00 (FERM BP-6394), Bacillus sp. OYK-03-60
0 (FERM BP-6395) and Bacillus sp. OYK-03-
No. 600 (FERM BP-6396) is used. These novel microorganisms have already been described in detail in Japanese Patent Application No. Hei 9-237974, and will not be described.

The cell preparation according to the present invention is used in the fermentation process of putrefactive waste or excrement to promote fermentation. The microorganisms described above are preferable in terms of environmental hygiene, because they have a high growth rate and do not generate an odor in the process of decomposing organic substances. In particular, in fermentation in the composting of livestock manure, the above-mentioned microorganisms rapidly grow without an induction period, so that the temperature of the compost is remarkably increased and the composting period can be shortened.
Since it has the effect of suppressing the bad smell of organic substances, it can be used as a deodorant, so it is also used for sanitary goods, diapers, and other articles dealing with excrement. Until the time of addition and mixing, microorganisms are sporulated and dormant, but germinate and proliferate when exposed to water.

DESCRIPTION OF THE PREFERRED EMBODIMENTS The present invention will be described based on embodiments. In each embodiment, 600 g of cow dung was placed in a thermos bottle, a thermometer was inserted, and the temperature was automatically collected at predetermined time intervals.

[0015]

Example 1 FIG. 1 shows the use of the bacterial cell preparation according to the present invention.
It shows the temperature change in the early stage of composting cow dung. In the figure, the samples of the curves with the respective reference numerals are as follows. The air supply is 20 cc / min. (A) A powder of OYK bacteria having a bacterial concentration of 1 × 10 ⁹ cells / g, diluted 100-fold with finely divided defatted soybeans, was added at a weight ratio of 0.2% to 600 g of cow dung, Further, 0.03% of protease of protease is added and stirred. (B) Remove protease from sample (A). (C) Only cow dung excluding OYK bacteria and protease from sample (A). Approximately 30 hours after the start of the test, each sample reaches the maximum temperature. The maximum temperature difference is obvious, and the temperature difference between the samples (A) and (B) is 8 ° C., and the samples (A) and (A) The temperature difference of C) actually reaches 19 ° C. OY
Bacterium K originally has the ability to secrete protein-degrading enzymes and degrade proteins to absorb nutrients such as nitrogen and carbon. However, there is no induction period during initial growth, and Since the growth starts rapidly, the more nutrients, the faster the growth rate will be. The defatted soybean added in the sample (A) is a plant protein source, and its surface area is larger in each stage than in the granular form because it has been pulverized. Dispersion is widespread and easy. Originally, animal dung contains various protein sources, so fermentation does not proceed under bad conditions where bacteria can not absorb those nutrients, so they are decomposed using enzymes that decompose nutrients. This allows
Since nutrients can be given to the OYK bacteria, the OYK bacteria start growing and fermentation is started. It is not possible to specify whether the added protease acted on the above defatted soybean protein source or on various protein sources contained in livestock dung. The effect of the protease on the OYK bacterium is remarkable, since no is observed. In order to absorb the protein source, it is necessary to decompose the protein to reduce its molecular weight. is there.

[0016]

Embodiment 2 FIG. 2 shows an example in which sodium nitrate (sodium nitrate) is added as an inorganic nitrogen source. In the figure, the samples of the curves with the respective reference numerals are as follows. The cow dung used was from a dairy cow tested by the National Agriculture and Forestry Technology Center.
6.1%. On the other hand, 9% (by weight) of coffee residue and 9% (by weight) of compost in the Kannan district with a moisture content of 40% were added to adjust the moisture content to 75%, and then subjected to the test. (D) A powder of OYK bacteria having a bacterial concentration of 1 × 10 ⁹ cells / g was diluted 100-fold with micronized defatted soybean to adjust the bacterial concentration to 1 × 10 ⁷ cells / g, and 600 g of cow dung 0.3% by weight with respect to. Further, sodium nitrate was added to 0.1%.
Add 3% and stir. (E) 0.3% of the above-mentioned bacterial concentration adjusted to 1 × 10 ⁷ cells / g and 1% of defatted soybean granules are added to cow dung and stirred. (F) Only cow dung of sample (D). For each sample, each additive was mixed with 600 g of cow dung, sealed in a pot, and the temperature change of the cow dung was measured. The air supply is 20 cc / min. Since sodium nitrate easily releases nitrogen, it is presumed that bacteria can be absorbed faster than nitrogen absorbed after decomposing soy protein. Sample (D) reached a maximum temperature in about 2 days, with a maximum temperature of 69.1 ° C. In contrast, sample (E) stopped at 65.9 ° C., and reached the maximum temperature about half a day later. The sample (F) is only cow dung, and its temperature rises slowly and its maximum temperature is as low as 50.9 ° C., and unfermented substances remain, so that the temperature drops slowly. Since the quality of the compost is better as the amount of unfermented material is smaller, the temperature is raised to 60 ° C or higher in a short time to promote the growth of actinomycetes, and promoting fermentation prevents the growth of other harmful bacteria. A compost with low odor can be obtained. The fermentation of the present embodiment is aerobic fermentation, and since temperature rise due to anaerobic fermentation is common, it has been assumed that conventional composting facilities suffer from bad odors. Composting can be performed without diverging.

[0017]

Embodiment 3 An example in which urea is used as an organic compound to supply a carbon source and a nitrogen source will be described. In this case, since a carbon source can also be supplied, a better result can be expected. Therefore, FIG. 3 shows a test result obtained by mixing only urea without adding another mixture. The cow dung used was from a dairy cow tested by the National Agriculture and Forestry Technology Center and had an initial moisture content of 86.1%. On the other hand, 9% (by weight) of coffee residue and 9% (by weight) of compost from the Kannan district with a moisture content of 40% were added to reduce the moisture content to 75%.
Was adjusted for the test. A sample having the following composition was prepared using a nutrient source of a synthesized chemical substance without using a natural nutrient source such as soybean protein. (G) OYK bacteria having a bacterial concentration of 1 × 10 ⁹ cells / g grown by liquid culture of nutrient broth were diluted with pulverized pulp to 1 × 10 ⁸ cells / g, and 0.1 g / g of cattle dung was collected. Add 24% and mix 0.6% urea. The result was put in a pot and subjected to an appropriate amount of ventilation, and the temperature was measured. The result is shown by a symbol (G) in FIG. The temperature reaches 47 ° C. approximately one day after the start of the test, but the temperature rise stops once. During this time, urea, which is a nutrient source, was consumed or leaked and OYK
It is presumed that the bacteria could not be absorbed. It is also determined that the bacterial flora has changed. After that, the temperature rose again, which is presumed to be the result of decomposing and growing the organic matter contained in the cow dung. Under such circumstances, an attempt was made to increase the temperature continuously by controlling the elution of urea over time.
Using a fluidized bed coating machine, urea microparticles are thinly coated with polyvinyl alcohol to make a slow-acting mixture that is brought into contact with the water of cow dung to elute in about one day, and mixed with unprocessed urea. The following is an example of the addition. (H) For 600 g of cow dung having the same configuration as (G),
0.4% by weight of urea and 0.4% of the above-mentioned slow-soluble urea
The temperature rise was measured in the same manner after addition and mixing, and a temperature curve indicated by the symbol (H) in FIG. 3 was obtained. The rising curve at the beginning of the measurement is slightly gentler than (G), but the temperature continuously rises. On the third day, a maximum temperature of 67.5 ° C. was recorded. This confirms that even if unprocessed ordinary urea was consumed as a nutrient source, there was an effect by continuing to supplement the nutrition with a slow-dissolving urea. Urea can be a source of nutrients not only for OYK bacteria but also for other bacteria contained in cow dung.
It is caused by metabolic energy that accompanies the rapid growth of bacteria and promotes the activity of thermophilic bacteria. In this example, a slow-acting urea was used, but a mixed solution of urea and polyvinyl alcohol was prepared and finely pulverized with a spray dryer to obtain a slow-acting urea. Can be dealt with.

The components of phosphorus and sulfur required for the growth of microorganisms may be supplied individually, but a compound containing both carbon and nitrogen is more workable because the amount of addition is very small. . Phosphate esters such as nucleosides are suitable for supplying phosphorus, and thiourea is preferred as a sulfur source. These compounds are suitable for the growth of OYK bacteria, but when actually added to and mixed with cow dung, it has been found that the temperature rise curve is slightly improved and shows a more stable temperature rise for different types of cow dung. I have. Cattle dung has different diets in warm and cold regions, and the chemicals mixed into the diets also make a difference in the components of cow dung. Furthermore, beef cattle and dairy cattle are raised differently, and the components of manure are different between drinking water and tap water and natural water. Therefore, the preparation is slightly changed depending on the conditions under which the cell preparation of the present invention is used.

[0019]

Embodiment 4 On the other hand, the following effects can be obtained by mixing or adding a peroxide compound as an oxygen supply source. When fermentation is started at an early stage and the temperature starts to gradually increase, the growth of microorganisms becomes more active and oxygen tends to be insufficient. As the peroxide compound, sodium peroxide carbonate, calcium peroxide and the like are preferably used.
In this embodiment, ground pulp is also used as a diluent for adjusting the bacterial concentration. FIG. 4 shows an example using sodium percarbonate and urea as a mixture. In the figure, the samples of each curve with reference numerals are as follows. (I) OYK bacteria having a bacterial concentration of 1 × 10 ⁹ cells / g grown by liquid culture of nutrient broth were diluted with pulverized pulp to adjust to 1 × 10 ⁷ cells / g. Add 1% and mix 0.15% defatted soybean with 0.15% urea. (J) The concentration of OYK bacteria obtained in the same manner as in (I) is adjusted to 1 × 10 ⁸ cells / g, and 1%, 1% sodium percarbonate and 1% citric acid are mixed. (K) Only OYK bacteria containing no mixture in the configuration of (J). (L) Only cow dung of sample (I). However, the cow dung used was from a dairy cow tested by the National Agriculture and Forestry Technology Center and had an initial moisture content of 86.1%. On the other hand, compost 9 in the Kannan district with 9% coffee ratio (weight ratio) and 40% moisture content
% (Weight ratio) and adjusted to a moisture content of 75% were subjected to the test. From these tests, it was found that when the bacterial concentration was diluted with ground pulp that was not a eutrophic source, it took about one extra day to reach the maximum temperature. FIG. 3 shows a case where only the situation where the maximum temperature is reached is taken every 30 minutes. Comparing samples (J) and (K) with respect to the effect of the oxygen supply source, the bacteria of sample (K) alone have a faster temperature rise immediately after the start of the test, but start to stagnate after 16 hours. This is considered to be due to lack of oxygen. On the other hand, in the sample (J), the temperature rise is gradual, but after 16 hours, the temperature rise exceeds that of the sample (K). Although the supply of oxygen was successful, the slow rise in temperature immediately after the start of the test is considered to be due to the effect of components generated when sodium percarbonate starts decomposing by contact with moisture. In any case, immediately after the start of the test, the sample is agitated in order to put the sample into the thermos, so that it is clear that air sufficiently intervenes, and it is understood that such a result was obtained. For sample (I), urea effectively acted as a nutrient for OYK bacteria. Although the bacterial concentration is one order of magnitude lower than that of the sample (K), the temperature rise is good.

From the above example, it can be seen that about two to
The temperature of the compost rises to a peak on the third day, at which point the growth is most advanced and there is a substantial lack of oxygen. Normally, when mixing the cell preparation of the present invention in the initial stage of fermentation, the compost is stirred and a large amount of air is involved, and the growth of the bacteria is performed and consumed using this air. Therefore, the extent to which the rise in temperature is extended depends on the amount of air initially contained. Conventionally, a method has been adopted in which when the fermentation temperature starts to decrease, the fermentation temperature is turned back and the temperature is increased again. From such a situation, it is convenient to add peroxide as an oxygen supply source at an early stage or at a time before the temperature rise reaches a peak. In the initial stage, when water is mixed at a high level, it reacts with water and decomposes with the generation of heat to generate oxygen. Therefore, it can contribute not only to an increase in the initial temperature but also to contain a large amount of oxygen. On the other hand, in order to have a delay of about 2 days, the peroxide granules are coated with an alkaline substance as a primary film, a polyolefin resin is coated as a secondary film, and the secondary film is formed of water. This is achieved by allowing the primary coating to elute when passing through and dissolving the secondary coating. Also, as the fermentation proceeds, a small amount of ammonia is generated during the growth of the bacteria, and the compost becomes alkaline, making use of the fact that the compound is alkaline and is soluble in alkaline water, such as hydroxypropylmethylcellulose phthalate and phthalate acetate. Acidic cellulose and the like are insoluble in water but are soluble in alkaline water and may be coated with these thin films. In any case,
The OYK bacterium used in the present invention grows well in a weak alkali, but cannot grow in a strong alkali. Therefore, a large amount of the above peroxide cannot be added and mixed. The addition amount is less than 1.5% based on the weight of the compost, preferably 1 to
The range is 0.5%. When the above peroxide is mixed,
Although the temperature peak is slightly higher, the high temperature range tends to be maintained, and it greatly contributes to the maturity as compost.

Also, a wax coating that controls the elution time as the temperature rises is advantageous because the oxygen deficiency time can be linked to the temperature change. This is because the rapid rise in temperature coincides with the time when the lack of oxygen is feared due to the growth. In fact, the nutrient sources contained in the feed differ depending on the production area, and the beef cattle and dairy cattle have different methods of raising and the composition of cow dung, so the bacterial cell preparation of the present invention also needs to adjust the mixing ratio accordingly. .
Therefore, in addition to determining the elution time when the slow-acting or slow-acting processing comes into contact with moisture, a technique in which elution is performed according to a rise in temperature of the compost to be composted is also extremely effective. This method is also applied to carbon compounds, nitrogen-containing compounds, phosphorus-containing compounds, sulfur-containing compounds, and the like, which are supplied as the above-mentioned nutrient sources.

[0022]

As described above, according to the microbial cell preparation of the present invention, the decomposition of feces of livestock and the like is promoted, and the fermentation heat upon decomposition is rapidly increased, so that the maturation degree is high and the harmful bacteria It is possible to obtain compost with less amount in a short time. This also contributes to promoting the decomposition of other putrefactive waste or excrement. In addition, the proteases and carbon compounds, nitrogen-containing compounds, phosphorus-containing compounds, and sulfur-containing compounds contained in the cell preparation of the present invention are also compounds used for chemical fertilizers, and the OYK bacteria used do not have hemolytic properties. The safety of bacillus tin butyls has been confirmed in animal experiments, and it is harmless even at the position where it touches human skin. Therefore, it is possible to suppress the odor of excrement and the growth of harmful bacteria and to keep the local area clean by using it as a sanitary article. In the embodiment, 6
Although the results of mixing various compounds with 00 g of cow dung were described, the effect was also confirmed in a field test using a large amount of cow dung in an actual composting lane,
When targeting a large amount of cow dung, there is a warming effect,
It has been confirmed that the maximum temperature rise is about 2 to 5 degrees higher than the experimental value described above. In addition, since the cell preparation of the present invention keeps the compost to be fermented at a fermentation temperature of 60 ° C. or higher, it is possible to provide a high-quality compost free of weed seeds and harmful bacteria.

[Brief description of the drawings]

FIG. 1 is a graph showing a temperature change when an enzyme is mixed.

FIG. 2 is a graph showing a temperature change when an inorganic nitrogen source is mixed.

FIG. 3 is a graph showing a temperature change when urea of an organic compound is mixed as a carbon source and a nitrogen source.

FIG. 4 is a graph showing a temperature change when an oxygen supply source is mixed.

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Claims (5)

[Claims] 1. Bacillus tin butyls-related bacterium which does not have hemolytic properties, has a larger cell volume than standard Bacillus subtilis (IFO-3134 strain), and has an initial culture in shaking culture of nutrient broth at 37 ° C. Has almost no induction period,
Due to the short-term growth, these synergistic effects degrade proteins, cellulose, starch, lipids, etc. into microorganisms characterized by the metabolizable energy generated by the growth being extremely large compared to that of the standard Bacillus subtilis. A cell preparation comprising one or more of the following enzymes mixed or added. 2. The microorganism according to claim 1, further comprising a carbon compound, a nitrogen-containing compound, a phosphorus-containing compound, a sulfur-containing compound, and / or
Or a cell preparation prepared by mixing or adding at least one or more processed compounds or mixtures in which the effect is controlled by subjecting these compounds to slow-acting processing or slow-acting processing. 3. An oxygen supply source comprising at least one oxygen-generating compound and / or a processed oxygen-generating compound or a mixture obtained by subjecting these compounds to slow-acting or slow-acting to control the development of their effects. The bacterial cell preparation according to claim 1, which is mixed or added. 4. The microorganism according to claim 1, wherein the microorganism is Bacillus s.
p. OYK-01-600 (FERM BP -6394), Bacillus sp.
OYK-03-600 (FERM BP-6395) and Bacillus
A cell preparation using a novel microorganism which is at least one selected from the group consisting of sp. OYK-03-600 (FERM BP-6396). 5. The bacterial cell preparation according to claim 1, which is used in a fermentation process of putrefactive waste or excrement to promote fermentation.