CN115530070A - Breeding method of new wheat variety - Google Patents
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- CN115530070A CN115530070A CN202211338273.8A CN202211338273A CN115530070A CN 115530070 A CN115530070 A CN 115530070A CN 202211338273 A CN202211338273 A CN 202211338273A CN 115530070 A CN115530070 A CN 115530070A
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- 241000209140 Triticum Species 0.000 title claims abstract description 70
- 235000021307 Triticum Nutrition 0.000 title claims abstract description 70
- 238000009395 breeding Methods 0.000 title claims abstract description 33
- 230000010152 pollination Effects 0.000 claims abstract description 59
- 230000001488 breeding effect Effects 0.000 claims abstract description 18
- 238000009966 trimming Methods 0.000 claims abstract description 6
- 210000005069 ears Anatomy 0.000 claims description 57
- 238000000034 method Methods 0.000 claims description 32
- 238000005520 cutting process Methods 0.000 claims description 18
- 230000008775 paternal effect Effects 0.000 claims description 12
- 238000009396 hybridization Methods 0.000 claims description 11
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 claims description 10
- 238000003825 pressing Methods 0.000 claims description 6
- 238000002054 transplantation Methods 0.000 claims description 6
- 241000196324 Embryophyta Species 0.000 claims description 5
- 235000009161 Espostoa lanata Nutrition 0.000 claims description 5
- 240000001624 Espostoa lanata Species 0.000 claims description 5
- 238000004140 cleaning Methods 0.000 claims description 5
- 210000003811 finger Anatomy 0.000 claims description 5
- 210000005224 forefinger Anatomy 0.000 claims description 5
- 210000004936 left thumb Anatomy 0.000 claims description 5
- 238000005201 scrubbing Methods 0.000 claims description 5
- 239000002689 soil Substances 0.000 claims description 4
- FFRBMBIXVSCUFS-UHFFFAOYSA-N 2,4-dinitro-1-naphthol Chemical compound C1=CC=C2C(O)=C([N+]([O-])=O)C=C([N+]([O-])=O)C2=C1 FFRBMBIXVSCUFS-UHFFFAOYSA-N 0.000 claims description 3
- 235000017166 Bambusa arundinacea Nutrition 0.000 claims description 3
- 235000017491 Bambusa tulda Nutrition 0.000 claims description 3
- 240000003433 Miscanthus floridulus Species 0.000 claims description 3
- 241000495841 Oenanthe oenanthe Species 0.000 claims description 3
- 235000015334 Phyllostachys viridis Nutrition 0.000 claims description 3
- 239000011425 bamboo Substances 0.000 claims description 3
- 239000000843 powder Substances 0.000 claims description 3
- 238000010008 shearing Methods 0.000 claims description 3
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 3
- 244000082204 Phyllostachys viridis Species 0.000 claims 1
- 238000004519 manufacturing process Methods 0.000 description 3
- 241001330002 Bambuseae Species 0.000 description 2
- 241001024327 Oenanthe <Aves> Species 0.000 description 2
- 238000001035 drying Methods 0.000 description 2
- 230000006872 improvement Effects 0.000 description 2
- 230000004075 alteration Effects 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 238000011161 development Methods 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 238000011156 evaluation Methods 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 238000009401 outcrossing Methods 0.000 description 1
- 108090000623 proteins and genes Proteins 0.000 description 1
- 238000005215 recombination Methods 0.000 description 1
- 230000006798 recombination Effects 0.000 description 1
- 230000009467 reduction Effects 0.000 description 1
- 238000006467 substitution reaction Methods 0.000 description 1
- 238000012360 testing method Methods 0.000 description 1
- 230000007704 transition Effects 0.000 description 1
Classifications
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- A01H1/00—Processes for modifying genotypes ; Plants characterised by associated natural traits
- A01H1/02—Methods or apparatus for hybridisation; Artificial pollination ; Fertility
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
- Y02A40/10—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in agriculture
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- General Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
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- Developmental Biology & Embryology (AREA)
- Environmental Sciences (AREA)
- Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
Abstract
The invention belongs to the technical field of crop breeding, and particularly relates to a breeding method of a new wheat variety, which comprises the steps of spike selection and ear trimming, castration and bagging, pollination and propagation.
Description
Technical Field
The invention relates to the technical field of crop breeding, in particular to a breeding method of a new wheat variety.
Background
The wheat variety improvement in China starts from the evaluation of local varieties, and a batch of good local varieties are evaluated from various places to be popularized and applied in production as transition; and then, foreign variety tests are introduced for trial breeding, and the hybrid is preferentially hybridized with local varieties to improve the varieties, along with the improvement of the production level of the wheat, the condition that the yield per unit is gradually increased appears in China and other wheat production countries, although the existing breeding method for new wheat varieties can breed wheat with better varieties, the soil condition and climate of each area are different, and the breeding method is different, so that the breeding method for the wheat which can be suitable for most areas is needed.
Disclosure of Invention
The invention aims to provide a breeding method of a new wheat variety, which solves the problem of reduction in yield and quality of wheat in the background technology.
In order to achieve the purpose, the invention provides the following technical scheme: a breeding method of a new wheat variety comprises the following steps:
step 1: selecting ears and trimming ears, selecting main stem ears of 4-6 robust and plump plants in the female parent population according to the determined hybridization combination, reserving 8-10 small ears in the middle of the main stem ears, removing small flowers at the upper parts of the small ears by using forceps, and reserving only two well-developed small flowers at the outer sides of the base parts;
step 2: removing stamens and bagging, namely cleaning anthers of each floret in the step 1, clamping wheat ears in the step 1 by using a left thumb and a middle finger during castration, slightly pressing the top of each glume by using a forefinger to separate the inner glume from the outer glume, scrubbing the tweezers by using an alcohol cotton ball, sequentially inserting the tweezers into the joint seams of the inner glume and the outer glume from top to bottom by using a right hand, slightly extruding three stamens by using the tweezers without clamping the anthers and damaging stigma, immediately bagging and isolating after castration is finished, and marking names and dates on the bags;
and 3, step 3: pollination, pollination is carried out within 1-3 days after castration in the step 2, when the pollination time is 8-11 am, the stigmas are checked to have no damage before pollination, when the stigmas are in feather-shaped bifurcation and glossy, which shows that the pollination period is right, the ear pollen of which the small flowers in the middle of the ears are opened in the male parent is selected to be put into the castrated flowers in an artificial or natural pollination mode, anthers are lightly smeared on the stigmas during pollination, the paper bags are sleeved after full ear pollination, the name of the male parent and the pollination date are written on the cards, and the paper bags are removed after 10 days;
and 4, step 4: breeding, performing vernalization treatment and generation-adding breeding on the wheat seeds of the 1 generation obtained in the step 3 to obtain wheat seeds of the 2 generation; vernalization and generational propagation are carried out on the wheat seeds of the 2 generations to obtain wheat seeds of the 3 generations; vernalization treatment and generation-adding propagation are carried out on the F3 generation wheat seeds to obtain 4 generation wheat seeds; vernalization treatment is carried out on the 4-generation wheat seeds, and directional cultivation is carried out to obtain a new variety of wheat.
Preferably, the main shoot spike in the step 1 is just drawn out of the leaf sheath, and the anther is green.
Preferably, when castration is performed on wheat in the step 2, if anthers break or the anthers are yellow, the flowers should be cut off, and the tweezers are wiped clean with alcohol, so as to avoid the phenomenon of powder stringing.
Preferably, the artificial pollination in the step 3 is to collect mature pollen of the male parent in a small cup or on paper, and then to put the pollen into each castrated flower in sequence by a pollinator immediately, wherein the mature pollen of the male parent is golden yellow in anther and has pollen to spill out.
Preferably, the natural pollination mode in the step 3 comprises a tassel twisting hybridization method and a paternal transplantation method, wherein the tassel twisting hybridization method comprises the following steps:
step A: removing tassels for the whole ear, removing tassels for the EF68 super wheat firstly, wherein the tasseling method is the same as the tasseling method in the step 2, then cutting miscanthus and glumes, and the cut glumes account for about 1/3-1/2 of the whole glumes, and taking the degree of not damaging stigma as a measure;
and B: treating the ear of the male parent before pollination, selecting the ear of the male parent from which anther of a small flower at the middle upper part is about to extend, then carrying out glume cutting treatment, cutting the ear of the male parent which is cut with glume, inserting the ear of the male parent into soil, and drying the ear of the male parent in the sun for 2-3 minutes, and carrying out pollination when the existing anther extends out of glume and is about to loose pollen;
and C: and (3) cutting the upper end of the paper bag sleeved in the step (2) by using scissors, moving the paternal ear processed in the step (B) to the position above the bag opening, opening the paper bag opening by using one hand, carefully turning the cut opening of the paternal ear downwards by using the other hand, inserting the cut opening into the paper bag, rotating to enable pollen to naturally fall onto the stigma of each flower, and folding and clamping the cut opening of the paper bag after pollination is finished.
The paternal transplantation method comprises the following steps:
the method comprises the following steps: emasculation is carried out on the whole ear, the emasculation is carried out on the EF68 super wheat firstly, and the emasculation method is consistent with the emasculation method in the step 2;
step two: pollinating, stripping 1-2 small ears in the middle part to see the pollen of the male parent which turns yellow but does not bloom, shearing 2-3 male parent ears from the second section of the basal part, inserting the male parent ears into a bottle which is bound on a bamboo pole and filled with water to ensure that the male parent ears uniformly surround the female parent ears, the male parent ears are higher than the female parent ears, slightly bundling thin lines, and then sleeving a large paper bag.
Compared with the prior art, the invention has the beneficial effects that:
the invention can improve the outcrossing rate, improve the breeding efficiency and promote the gene recombination among varieties, thereby breeding the wheat with high-quality varieties.
Detailed Description
The technical solutions in the embodiments of the present invention will be described clearly and completely below, and it is obvious that the described embodiments are only a part of the embodiments of the present invention, and not all of the embodiments. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.
In the description of the present invention, it is to be understood that the terms "upper", "lower", "front", "rear", "left", "right", "top", "bottom", "inner", "outer", and the like are used merely for convenience in describing and simplifying the description, and do not indicate or imply that the device or element referred to must have a particular orientation, be constructed and operated in a particular orientation, and thus, should not be taken as limiting the invention.
The invention provides a technical scheme that:
example 1:
a breeding method of a new wheat variety comprises the following steps:
step 1: selecting ears and trimming ears, selecting main stem ears of 4 robust and plump plants in a female parent group according to a determined hybridization combination, remaining 8 small ears in the middle of the main stem ears, removing small flowers at the upper parts of the small ears by using forceps, and only remaining two small flowers with good development outside a basal part;
step 2: removing stamens and bagging, namely cleaning the anther of each floret in the step 1, clamping the wheat ear in the step 1 by using a left thumb and a middle finger during castration, slightly pressing the top of an outer glume by using a forefinger to separate the inner glume from the outer glume, scrubbing the tweezers by using an alcohol cotton ball, sequentially inserting the tweezers into the joint of the inner glume and the outer glume from top to bottom by using a right hand, slightly cutting out three stamens by using the tweezers without breaking the anther and damaging a stigma, immediately bagging and isolating after castration is finished, marking a name and a date on the bag, cutting off the flower if the anther is broken or the anther is yellow during castration of the wheat, and wiping the tweezers by using alcohol to avoid the pollen stringing phenomenon;
and step 3: pollination, pollination is carried out within 1 day after castration in the step 2, when the pollination time is 8 am, the stigma is checked to have no damage before pollination, when the stigma is feather-shaped and forked and glossy, which shows that the pollination period is right, the ear pollen of which the small flowers in the middle of the ear in the male parent are opened is selected to be put into the castrated flower in an artificial or natural pollination mode, anthers are lightly smeared on the stigma during pollination, a paper bag is sleeved after full ear pollination, the name of the male parent and the pollination date are written on the card, and the paper bag is removed after 10 days;
and 4, step 4: breeding, performing vernalization treatment and generation-adding breeding on the wheat seeds of the 1 generation obtained in the step 3 to obtain wheat seeds of the 2 generation; vernalization and generational propagation are carried out on the wheat seeds of the 2 generations to obtain wheat seeds of the 3 generations; vernalization treatment and generation-adding propagation are carried out on the F3 generation wheat seeds to obtain 4 generation wheat seeds; vernalization treatment is carried out on the 4-generation wheat seeds, and directional cultivation is carried out to obtain a new variety of wheat.
Example 2:
a breeding method of a new wheat variety comprises the following steps:
step 1: selecting ears and trimming ears, selecting main stem ears of 5 robust and plump plants in the female parent population according to the determined hybridization combination, reserving 9 small ears in the middle of the main stem ears, removing small flowers at the upper parts of the small ears by using forceps, and reserving only two small flowers which are well developed at the outer sides of the base parts;
step 2: removing stamens and bagging, namely cleaning the anther of each floret in the step 1, clamping the wheat ear in the step 1 by using a left thumb and a middle finger during castration, slightly pressing the top of an outer glume by using a forefinger to separate the inner glume from the outer glume, scrubbing the tweezers by using an alcohol cotton ball, sequentially inserting the tweezers into the joint of the inner glume and the outer glume from top to bottom by using a right hand, slightly cutting out three stamens by using the tweezers without breaking the anther and damaging a stigma, immediately bagging and isolating after castration is finished, marking a name and a date on the bag, cutting off the flower if the anther is broken or the anther is yellow during castration of the wheat, and wiping the tweezers by using alcohol to avoid the pollen stringing phenomenon;
and 3, step 3: pollination, pollination is carried out within 2 days after castration in the step 2, when the pollination time is 10 am, the stigma is checked to have no damage before pollination, when the stigma is feather-shaped and forked and glossy, which shows that the pollination period is right, the ear pollen of which the small flowers in the middle of the ear in the male parent are opened is selected to be put into the castrated flower in an artificial or natural pollination mode, anthers are lightly smeared on the stigma during pollination, a paper bag is sleeved after full ear pollination, the name of the male parent and the pollination date are written on the card, and the paper bag is removed after 10 days;
and 4, step 4: breeding, performing vernalization treatment and generation-adding breeding on the wheat seeds of the 1 generation obtained in the step 3 to obtain wheat seeds of the 2 generation; vernalization and generational propagation are carried out on the wheat seeds of the 2 generations to obtain wheat seeds of the 3 generations; vernalizing and breeding the F3 generation wheat seeds to obtain 4 generation wheat seeds; vernalization treatment is carried out on the 4-generation wheat seeds, and directional cultivation is carried out to obtain new wheat varieties.
Example 3:
a breeding method of a new wheat variety comprises the following steps:
step 1: selecting ears and trimming ears, selecting main stem ears of 6 robust and plump plants in the female parent population according to the determined hybridization combination, reserving 10 small ears in the middle of the main stem ears, removing the upper small flowers of the small ears by using forceps, and reserving only two well-developed small flowers outside the base;
and 2, step: removing the stamens and bagging, cleaning the anther of each floret in the step 1, clamping the wheat ears in the step 1 by using a left thumb and a middle finger during castration, slightly pressing the top of the glume with a forefinger to separate the glume from the glume, scrubbing the tweezers by using an alcohol cotton ball, sequentially inserting the tweezers into the joint of the glume from top to bottom by using the tweezers on the right hand, slightly cutting three stamens by using the tweezers without clamping the anther and damaging the stigma, immediately bagging and isolating after castration is finished, marking the name and the date on the bag, cutting the flower when the anther is broken or the anther is yellow during castration of the wheat, and wiping the tweezers by using alcohol to avoid the phenomenon of powder stringing;
and step 3: pollination is carried out within 3 days after castration in the step 2, when the pollination time is 11 am, the stigmas are not damaged, when the stigmas are in feather-shaped bifurcation and glossy, which shows that the stigmas are in the pollination period, the ear pollen of which the small flowers in the middle of the ears in the male parent are opened is selected to be put into the castrated flowers in a manual or natural pollination mode, anthers are lightly smeared on the stigmas during pollination, a paper bag is sleeved after full ear pollination, the name of the male parent and the pollination date are written on the cards, and the paper bag is removed after 10 days;
and 4, step 4: breeding, performing vernalization treatment and generation-adding breeding on the wheat seeds of the 1 generation obtained in the step 3 to obtain wheat seeds of the 2 generation; vernalization and generational propagation are carried out on the wheat seeds of the 2 generations to obtain wheat seeds of the 3 generations; vernalization treatment and generation-adding propagation are carried out on the F3 generation wheat seeds to obtain 4 generation wheat seeds; vernalization treatment is carried out on the 4-generation wheat seeds, and directional cultivation is carried out to obtain a new variety of wheat.
The artificial pollination mode is that mature male parent pollen is collected in a small cup or on paper, and then pollen is immediately put into each castrated flower in sequence by a pollinator, wherein the mature male parent pollen is golden yellow in anther and has pollen to spill out; the natural pollination mode comprises a ear-twisting hybridization method and a male parent transplantation method, wherein the ear-twisting hybridization method comprises the following steps:
step A: removing tassels for the whole ear, removing tassels for the EF68 super wheat firstly, wherein the tasseling method is the same as the tasseling method in the step 2, then cutting miscanthus and glumes, and the cut glumes account for about 1/3-1/2 of the whole glumes, and taking the degree of not damaging stigma as a measure;
and B: treating the ear of the male parent before pollination, selecting the ear of the male parent from which anther of a small flower at the middle upper part is about to extend, then carrying out glume cutting treatment, cutting the ear of the male parent which is cut with glume, inserting the ear of the male parent into soil, and drying the ear of the male parent in the sun for 2-3 minutes, and carrying out pollination when the existing anther extends out of glume and is about to loose pollen;
and C: and (3) cutting the upper end of the paper bag sleeved in the step (2) by using scissors, moving the paternal ears processed in the step (B) to the positions above the bag openings, opening the paper bag openings by using one hand, carefully turning the cut openings of the paternal ears downwards by using the other hand, inserting the paternal ears into the paper bag, rotating to enable the pollen to naturally fall onto the stigma of each flower, and folding and clamping the cut openings of the paper bag after pollination is finished.
The paternal transplantation method comprises the following steps:
the method comprises the following steps: emasculation is carried out on the whole ear, the emasculation is carried out on the EF68 super wheat firstly, and the emasculation method is consistent with the emasculation method in the step 2;
step two: pollinating, stripping 1-2 small ears in the middle part to see the pollen of the male parent which turns yellow but does not bloom, shearing 2-3 male parent ears from the second section of the basal part, inserting the male parent ears into a bottle which is bound on a bamboo pole and filled with water to ensure that the male parent ears uniformly surround the female parent ears, the male parent ears are higher than the female parent ears, slightly bundling thin lines, and then sleeving a large paper bag.
While there have been shown and described what are at present considered to be the basic principles and essential features of the invention and advantages thereof, it will be apparent to those skilled in the art that the invention is not limited to the details of the foregoing illustrative embodiments, but is capable of other embodiments without departing from the spirit or essential characteristics thereof; the present embodiments are therefore to be considered in all respects as illustrative and not restrictive, the scope of the invention being indicated by the appended claims rather than by the foregoing description, and all changes which come within the meaning and range of equivalency of the claims are therefore intended to be embraced therein.
Although embodiments of the present invention have been shown and described, it will be appreciated by those skilled in the art that changes, modifications, substitutions and alterations can be made in these embodiments without departing from the principles and spirit of the invention, the scope of which is defined in the appended claims and their equivalents.
Claims (5)
1. A breeding method of a new wheat variety is characterized by comprising the following steps: the method comprises the following steps:
step 1: selecting ears and trimming ears, selecting main stem ears of 4-6 robust and plump plants in the female parent population according to the determined hybridization combination, reserving 8-10 small ears in the middle of the main stem ears, removing small flowers at the upper parts of the small ears by using forceps, and reserving only two well-developed small flowers at the outer sides of the base parts;
and 2, step: removing the stamens and bagging, namely cleaning the anther of each floret in the step 1, clamping the wheat ear in the step 1 by using a left thumb and a middle finger during castration, slightly pressing the top of the glume with a forefinger to separate the glume from the glume, scrubbing the tweezers by using an alcohol cotton ball, sequentially inserting the tweezers into the joint of the glume from top to bottom by using the tweezers on the right hand, slightly pressing the tweezers to generate three stamens without clamping the anther and damaging the stigma, immediately bagging and isolating after castration is finished, and marking the name and the date on the bag;
and step 3: pollination, pollination is carried out within 1-3 days after castration in the step 2, when the pollination time is 8-11 am, the stigmas are checked to have no damage before pollination, when the stigmas are in feather-shaped bifurcation and glossy, which shows that the pollination period is right, the ear pollen of which the small flowers in the middle of the ears are opened in the male parent is selected to be put into the castrated flowers in an artificial or natural pollination mode, anthers are lightly smeared on the stigmas during pollination, the paper bags are sleeved after full ear pollination, the name of the male parent and the pollination date are written on the cards, and the paper bags are removed after 10 days;
and 4, step 4: breeding, performing vernalization treatment and generation-adding breeding on the wheat seeds of the 1 generation obtained in the step 3 to obtain wheat seeds of the 2 generation; vernalizing and breeding the wheat seeds of the 2 generation to obtain wheat seeds of the 3 generation; vernalizing and breeding the F3 generation wheat seeds to obtain 4 generation wheat seeds; vernalization treatment is carried out on the 4-generation wheat seeds, and directional cultivation is carried out to obtain new wheat varieties.
2. The method of claim 1, wherein the method comprises the following steps: the main stem spike in the step 1 is just drawn out of the leaf sheath, and the anther is green.
3. The method of claim 1, wherein the method comprises the steps of: in the step 2, when the midlings are castrated, if the anthers are cracked or yellow, the flowers are cut off, and the tweezers are wiped clean by alcohol to avoid the phenomenon of powder stringing.
4. A breeding method of a new wheat variety according to any one of claims 1 to 3, characterized in that: and the artificial pollination mode in the step 3 is to collect mature male parent pollen in a small cup or on paper, and then put the pollen into each castrated flower in sequence by using a pollinator immediately, wherein the mature male parent pollen is golden yellow in anther and has pollen to spill out.
5. A breeding method of a new wheat variety according to claim 4, characterized in that: the natural pollination mode in the step 3 comprises a tassel twisting hybridization method and a paternal transplantation method, wherein the tassel twisting hybridization method comprises the following steps:
step A: removing tassels for the whole ear, removing tassels for the EF68 super wheat firstly, wherein the tasseling method is the same as the tasseling method in the step 2, then cutting miscanthus and glumes, and the cut glumes account for about 1/3-1/2 of the whole glumes, and taking the degree of not damaging stigma as a measure;
and B: treating the male parent ear before pollination, selecting the male parent ear from which anther of a small flower at the middle upper part is about to extend, then carrying out glume cutting treatment, cutting the glume-cut male parent ear, inserting the tasme into soil, and airing for 2-3 minutes in the sun, and carrying out pollination when the existing anther extends out of glume and is about to break pollen;
step C: and (3) cutting the upper end of the paper bag sleeved in the step (2) by using scissors, moving the paternal ear processed in the step (B) to the position above the bag opening, opening the paper bag opening by using one hand, carefully turning the cut opening of the paternal ear downwards by using the other hand, inserting the cut opening into the paper bag, rotating to enable pollen to naturally fall onto the stigma of each flower, and folding and clamping the cut opening of the paper bag after pollination is finished.
The paternal transplantation method comprises the following steps:
the method comprises the following steps: emasculation is carried out on the whole ear, the emasculation is carried out on the EF68 super wheat firstly, and the emasculation method is consistent with the emasculation method in the step 2;
step two: pollinating, stripping 1-2 small ears in the middle part to see the pollen of the male parent which turns yellow but does not bloom, shearing 2-3 male parent ears from the second section of the basal part, inserting the male parent ears into a bottle which is bound on a bamboo pole and filled with water to ensure that the male parent ears uniformly surround the female parent ears, the male parent ears are higher than the female parent ears, slightly bundling thin lines, and then sleeving a large paper bag.
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN116369195A (en) * | 2023-05-11 | 2023-07-04 | 安新县益农农业科技有限公司 | Seed selection method for wheat with big spike head |
| CN116508645A (en) * | 2023-04-17 | 2023-08-01 | 安新县益农农业科技有限公司 | Crossbreeding method of new wheat strain |
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| US20080289061A1 (en) * | 2001-02-09 | 2008-11-20 | Penner Greg A | Identification of seeds or plants using phenotypic markers |
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