CN115517087A - Method for rooting twigs by cutting plum blossom - Google Patents
Method for rooting twigs by cutting plum blossom Download PDFInfo
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- 238000005520 cutting process Methods 0.000 title claims abstract description 61
- 238000000034 method Methods 0.000 title claims abstract description 28
- 241000732800 Cymbidium Species 0.000 claims abstract description 34
- SEOVTRFCIGRIMH-UHFFFAOYSA-N indole-3-acetic acid Chemical compound C1=CC=C2C(CC(=O)O)=CNC2=C1 SEOVTRFCIGRIMH-UHFFFAOYSA-N 0.000 claims abstract description 19
- 239000001963 growth medium Substances 0.000 claims abstract description 16
- 239000002689 soil Substances 0.000 claims abstract description 16
- 241000196324 Embryophyta Species 0.000 claims abstract description 13
- 238000005286 illumination Methods 0.000 claims abstract description 12
- 239000007864 aqueous solution Substances 0.000 claims abstract description 9
- 239000003617 indole-3-acetic acid Substances 0.000 claims abstract description 7
- 230000035699 permeability Effects 0.000 claims abstract description 6
- 238000010008 shearing Methods 0.000 claims abstract description 4
- 239000000243 solution Substances 0.000 claims description 26
- 239000000645 desinfectant Substances 0.000 claims description 20
- 239000000843 powder Substances 0.000 claims description 20
- 238000004659 sterilization and disinfection Methods 0.000 claims description 20
- 239000002985 plastic film Substances 0.000 claims description 12
- 229920006255 plastic film Polymers 0.000 claims description 12
- 230000001954 sterilising effect Effects 0.000 claims description 10
- XSQUKJJJFZCRTK-UHFFFAOYSA-N Urea Chemical compound NC(N)=O XSQUKJJJFZCRTK-UHFFFAOYSA-N 0.000 claims description 7
- 239000004202 carbamide Substances 0.000 claims description 7
- PRPINYUDVPFIRX-UHFFFAOYSA-N 1-naphthaleneacetic acid Chemical compound C1=CC=C2C(CC(=O)O)=CC=CC2=C1 PRPINYUDVPFIRX-UHFFFAOYSA-N 0.000 claims description 5
- 235000017166 Bambusa arundinacea Nutrition 0.000 claims description 5
- 235000017491 Bambusa tulda Nutrition 0.000 claims description 5
- 241001330002 Bambuseae Species 0.000 claims description 5
- TWFZGCMQGLPBSX-UHFFFAOYSA-N Carbendazim Natural products C1=CC=C2NC(NC(=O)OC)=NC2=C1 TWFZGCMQGLPBSX-UHFFFAOYSA-N 0.000 claims description 5
- 235000015334 Phyllostachys viridis Nutrition 0.000 claims description 5
- 239000011425 bamboo Substances 0.000 claims description 5
- JNPZQRQPIHJYNM-UHFFFAOYSA-N carbendazim Chemical compound C1=C[CH]C2=NC(NC(=O)OC)=NC2=C1 JNPZQRQPIHJYNM-UHFFFAOYSA-N 0.000 claims description 5
- 239000006013 carbendazim Substances 0.000 claims description 5
- 239000012286 potassium permanganate Substances 0.000 claims description 5
- 210000002615 epidermis Anatomy 0.000 claims description 4
- 238000005507 spraying Methods 0.000 claims description 4
- 241000173290 Cymbidium faberi Species 0.000 abstract description 15
- 229930192334 Auxin Natural products 0.000 abstract description 5
- 239000002363 auxin Substances 0.000 abstract description 5
- 230000004083 survival effect Effects 0.000 abstract description 5
- 238000007598 dipping method Methods 0.000 abstract description 3
- 239000000758 substrate Substances 0.000 description 9
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 8
- 239000000284 extract Substances 0.000 description 6
- 238000004519 manufacturing process Methods 0.000 description 6
- 238000009395 breeding Methods 0.000 description 4
- 239000000463 material Substances 0.000 description 4
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- 230000009286 beneficial effect Effects 0.000 description 3
- 230000001488 breeding effect Effects 0.000 description 3
- 239000003337 fertilizer Substances 0.000 description 3
- 238000007654 immersion Methods 0.000 description 3
- 230000002401 inhibitory effect Effects 0.000 description 3
- 238000004080 punching Methods 0.000 description 3
- 239000000126 substance Substances 0.000 description 3
- ZRJBHWIHUMBLCN-SEQYCRGISA-N Huperzine A Natural products N1C(=O)C=CC2=C1C[C@H]1/C(=C/C)[C@]2(N)CC(C)=C1 ZRJBHWIHUMBLCN-SEQYCRGISA-N 0.000 description 2
- ZRJBHWIHUMBLCN-UHFFFAOYSA-N Shuangyiping Natural products N1C(=O)C=CC2=C1CC1C(=CC)C2(N)CC(C)=C1 ZRJBHWIHUMBLCN-UHFFFAOYSA-N 0.000 description 2
- 229930003451 Vitamin B1 Natural products 0.000 description 2
- 230000005059 dormancy Effects 0.000 description 2
- ZRJBHWIHUMBLCN-YQEJDHNASA-N huperzine A Chemical compound N1C(=O)C=CC2=C1C[C@H]1\C(=C/C)[C@]2(N)CC(C)=C1 ZRJBHWIHUMBLCN-YQEJDHNASA-N 0.000 description 2
- 239000007788 liquid Substances 0.000 description 2
- 230000008569 process Effects 0.000 description 2
- ZRJBHWIHUMBLCN-BMIGLBTASA-N rac-huperzine A Natural products N1C(=O)C=CC2=C1C[C@@H]1C(=CC)[C@@]2(N)CC(C)=C1 ZRJBHWIHUMBLCN-BMIGLBTASA-N 0.000 description 2
- 238000002791 soaking Methods 0.000 description 2
- 229960003495 thiamine Drugs 0.000 description 2
- DPJRMOMPQZCRJU-UHFFFAOYSA-M thiamine hydrochloride Chemical compound Cl.[Cl-].CC1=C(CCO)SC=[N+]1CC1=CN=C(C)N=C1N DPJRMOMPQZCRJU-UHFFFAOYSA-M 0.000 description 2
- YWYZEGXAUVWDED-UHFFFAOYSA-N triammonium citrate Chemical compound [NH4+].[NH4+].[NH4+].[O-]C(=O)CC(O)(CC([O-])=O)C([O-])=O YWYZEGXAUVWDED-UHFFFAOYSA-N 0.000 description 2
- 239000011691 vitamin B1 Substances 0.000 description 2
- 235000010374 vitamin B1 Nutrition 0.000 description 2
- 240000004539 Alocasia cucullata Species 0.000 description 1
- 241001070941 Castanea Species 0.000 description 1
- 235000014036 Castanea Nutrition 0.000 description 1
- 241000530047 Herpetospermum pedunculosum Species 0.000 description 1
- 244000241838 Lycium barbarum Species 0.000 description 1
- 235000015459 Lycium barbarum Nutrition 0.000 description 1
- 235000015468 Lycium chinense Nutrition 0.000 description 1
- 244000018633 Prunus armeniaca Species 0.000 description 1
- 235000009827 Prunus armeniaca Nutrition 0.000 description 1
- 235000011158 Prunus mume Nutrition 0.000 description 1
- 244000018795 Prunus mume Species 0.000 description 1
- 230000004075 alteration Effects 0.000 description 1
- 239000003086 colorant Substances 0.000 description 1
- 230000007547 defect Effects 0.000 description 1
- 235000013399 edible fruits Nutrition 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 230000007613 environmental effect Effects 0.000 description 1
- 230000004720 fertilization Effects 0.000 description 1
- 230000035784 germination Effects 0.000 description 1
- 150000004676 glycans Chemical class 0.000 description 1
- 238000009403 interspecific hybridization Methods 0.000 description 1
- 239000008518 lycium barbarum polysaccharide Substances 0.000 description 1
- 230000002503 metabolic effect Effects 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
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- 229920001282 polysaccharide Polymers 0.000 description 1
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- 230000008929 regeneration Effects 0.000 description 1
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Classifications
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G2/00—Vegetative propagation
- A01G2/10—Vegetative propagation by means of cuttings
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G17/00—Cultivation of hops, vines, fruit trees, or like trees
- A01G17/005—Cultivation methods
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- Life Sciences & Earth Sciences (AREA)
- Botany (AREA)
- Environmental Sciences (AREA)
- Developmental Biology & Embryology (AREA)
- Cultivation Of Plants (AREA)
Abstract
The invention belongs to the field of plant cuttage, and particularly discloses a method for rooting plum blossom through twig cuttage, which comprises the following steps: selecting soil which is loose and fertile, has good illumination and good drainage and air permeability as a slotting bed, wherein the length of the slotting bed is 1.5m, and the width of the slotting bed is 1m; selecting current-year semi-grown lignified plum blossom twigs with the time of 4-5 months, selecting a twigs section with the length of 12-13cm from the upper section of the plum blossom twigs as a cymbidium hyridus, wherein the upper end of the cymbidium hyridus is a plain mouth, and the middle part of the lower end of the cymbidium hyridus is cut into a V-shaped structure, so that the shearing surface is smooth and neat; the method selects the current-year semi-grown lignified plum blossom tender branches for 4-5 months, cuts the upper branch section as the cymbidium faberi Rolfe, thereby avoiding the problem that the branch section is too tender and easy to rot or too old and the auxin is reduced, achieving the seed selection condition easy to take root, and then quickly dipping the cymbidium faberi Rolfe in 500mg/kg indoleacetic acid aqueous solution and then inserting the cymbidium faberi into the culture medium in a culture dish for seedling culture, thereby effectively improving the survival rate of seedlings and improving the plum blossom cutting seedling quality.
Description
Technical Field
The invention relates to the field of plant cuttage, in particular to a method for rooting plum blossom by twig cuttage.
Background
Plum blossom (Prunus mume) is one of ten traditional flowers in China, has bright and colorful flower colors and rich branch type variation, and is an important ornamental flower tree and fruit tree. The plum blossom cultivation range is limited to the Yangtze river and the Huai river basin for more than 2000 years, and in order to expand the cultivation area range of the traditional famous flower, especially to popularize and apply the plum blossom to the northwest China, the cold-resistant plum blossom breeding work is already carried out before the half century. In the early stage, the variety of the plum blossom with the cold resistance is cultivated by interspecific hybridization of the variety of the true plum line and the kindred species such as apricot and the like, and the plant landscape in early spring in the north is enriched.
The breeding method of the plum blossom mainly comprises the following steps: seeding, grafting, cuttage and the like. Although the sowing and breeding are simple and easy, the offspring can not keep the excellent characteristics of the plum blossom breed. The production adopts a grafting method, and grafting is usually carried out in a plum blossom dormancy stage by bud grafting, cleft grafting or bark grafting. The selection of the grafting period has great influence on the survival rate, and the labor cost of grafting, bud picking, wind prevention, fixation and the like is high.
The branch cutting propagation can breed more seedlings in a short time and can keep the characteristics of good varieties, but because the survival rate of plum blossom cutting propagation is low, the plum blossom cutting propagation is not generally adopted in production. The invention with publication number CN106665243A discloses a plum blossom cutting seedling method, which takes semi-lignified branches in ten days of 11 months as cutting strips, the cutting length is 13-15 cm, 8-9 cm of the base parts of the cutting strips are firstly soaked in a soaking solution prepared from a Chinese rice-water chestnut extract and vitamin B1 for 84-88 min, then soaked in a lycium barbarum polysaccharide aqueous solution for 37-39 min, then the base parts are wrapped by a plastic film and then placed at the temperature of 37-39 ℃ for 6-7 h to obtain the cutting strips; cutting into seedling substrate, keeping the seedling shed shade all day, humidity of 86-90%, naturally illuminating for 320-340min after 10 days, and applying fertilizer solution prepared from semen Herpetospermi extract, herba Bulgaria extract, huperzine A, urea and ammonium citrate to the root after 20 days. The method has the following defects:
1. material taking: the material is obtained in the last ten days of 11 months, and semi-lignified branches 13-15 cm long are used as cuttings. Because branches in dormant seasons are used as scions, nutrients are only consumed and accumulated, metabolic activity of plants is slowed, and root regeneration and bud germination are not facilitated. The scions are long and cannot save materials well. Meanwhile, due to the temperature difference in North China and south China, the plant dormancy period has great difference, and the setting of the material taking time is not beneficial to wide popularization.
2. Pretreatment: before cutting, the cutting slips are soaked in a soaking solution prepared from a plant and river vehicle extract and vitamin B1 for 84-88 min, then soaked in a wolfberry polysaccharide aqueous solution for 37-39 min, then wrapped by a plastic film at the base part and put at 37-39 ℃ for 6-7 h for pretreating the cutting slips. The whole treatment process is long in time and complex in operation, and the propagation period and the production cost are increased.
3. And (3) environmental control: keeping the seedling shed shade all day after cuttage, the humidity is 86% -90%, after 10 days, natural illumination is carried out for 320-340min each day, and after 20 days, fertilizer liquid made of herpetospermum pedunculosum seed extract, alocasia cucullata extract, huperzine A, urea, ammonium citrate and the like is applied to the root. The labor cost of shading, single-plant fertilization, continuous pinching and the like all day is high, the components of the fertilizer liquid are complex and are not suitable for purchase, the production cost is high, and the method is not suitable for wide popularization.
The cutting seedling raising method is complex in process, high in production cost, and incapable of meeting the requirements of actual production and scientific research and breeding of the plum blossom without considering the cutting survival rate among varieties, so that the invention provides a method for efficiently and simply obtaining plum blossom cutting seedlings.
Disclosure of Invention
The invention aims to provide a method for rooting twigs by cutting plum blossom so as to solve the problems in the background technology.
In order to achieve the purpose, the invention provides the following technical scheme: a method for rooting twigs by cutting plum blossom comprises the following steps:
s1: selecting soil which is loose and fertile, has good illumination and good drainage and air permeability as a slotting bed, wherein the length of the slotting bed is 1.5m, and the width of the slotting bed is 1m;
s2: selecting annual semi-grown lignified plum blossom twigs for 4-5 months, selecting a twigs section with the length of 12-13cm from the upper section of each plum blossom twig as a cymbidium, cutting the middle part of the lower end of the cymbidium into a V-shaped structure, and ensuring that the shearing surface is smooth and regular;
s3: preparing a disinfectant, namely putting the lower end of the cymbidium hyridus into the disinfectant for disinfection and sterilization, so as to ensure that the V-shaped structure of the lower end of the cymbidium hyridus is completely immersed into the disinfectant, wherein the disinfection and sterilization time is 5-10min;
s4: preparing rooting powder, and after the cymbidium hyridus is sterilized, putting the cymbidium hyridus into the rooting powder, and ensuring that the lower V-shaped structure of the cymbidium hyridus completely enters the rooting powder for 30min;
s5: arranging a culture medium in a culture dish, obliquely inserting cymbidium into the culture medium, covering a plastic film on the surface of the culture medium, and putting the culture dish into an incubator for culture, wherein the ambient temperature in the incubator is 23-27 ℃;
s6: after the root system of the seedling grows completely, cutting the seedling into a cutting bed, wherein the plant spacing of each adjacent seedling is 3-5cm, the row spacing is 14-16cm, and the upper end of the seedling leaks 2-3cm from the ground;
s7: compacting the soil around the seedlings, watering at proper time to ensure the soil to be moist, and obtaining the plum blossom plants.
Preferably, the disinfectant is prepared by mixing a 10% potassium permanganate solution and 50% carbendazim.
Preferably, the rooting powder adopts 100-200ppm of naphthylacetic acid.
Preferably, the culture medium is prepared by mixing one or more of an ATP solution of 50-150mg/kg, an NAA solution of 100-150mg/kg or an IBA solution of 100-150 mg/kg.
Preferably, in the step S5, the cymbidium faberi is dipped into 500mg/kg of indoleacetic acid aqueous solution and then obliquely inserted into the culture medium.
Preferably, in the step S6, before the seedlings are cut, holes are punched at corresponding positions of the cutting bed by bamboo sticks, so as to prevent the surface of the seedlings from being damaged and rotten during the cutting process.
Preferably, in the step S6, after the root system of the seedling grows completely, the plastic film is gradually uncovered for 10 days to prolong the illumination time of the seedling.
Preferably, in the step S6, 0.1-0.2% of urea solution is sprayed every 7-10 days after the seedlings are planted in the cutting bed.
Preferably, the environment temperature of the slotting machine is 21-24 ℃, the relative humidity is 80-85%, and the shading degree is 70%.
Compared with the prior art, the invention has the beneficial effects that: the method selects the current-year semi-grown lignified plum blossom tender branches for 4-5 months, cuts the upper branch section as the cymbidium faberi Rolfe, thereby avoiding the problem that the branch section is too tender and easy to rot or too old and the auxin is reduced, achieving the seed selection condition easy to take root, and then quickly dipping the cymbidium faberi Rolfe in 500mg/kg indoleacetic acid aqueous solution and then inserting the cymbidium faberi into the culture medium in a culture dish for seedling culture, thereby effectively improving the survival rate of seedlings and improving the plum blossom cutting seedling quality.
Detailed Description
The technical solutions in the embodiments of the present invention will be clearly and completely described below, and it is obvious that the described embodiments are only a part of the embodiments of the present invention, and not all embodiments. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.
Example 1:
the invention provides a method for rooting twigs by cutting plum blossom, which comprises the following steps:
s1: soil which is loose and fertile, good in illumination and good in water drainage and air permeability is selected as the slotting machine, the quincuncial has high water demand but cannot resist water accumulation, and the slotting machine is 1.5m long and 1m wide;
s2: selecting annual semi-lignified plum blossom twigs which are 4 th of the middle ten days of the month, selecting branches which are too tender and too lignified, are not good, are too tender and easy to rot, have too old auxin reduced, have increased rooting inhibiting substance content and are not beneficial to rooting, selecting branch sections with the length of 12-13cm in the middle upper section of the plum blossom twigs as cymbidium, cutting the middle part of the lower end of the cymbidium into a V-shaped structure, and ensuring that the shearing surface is smooth and regular;
s3: preparing a disinfectant, wherein the disinfectant is formed by mixing a 10% potassium permanganate solution and 50% carbendazim, the lower end of the cymbidium faberi is put into the disinfectant for disinfection and sterilization, the V-shaped structure at the lower end of the cymbidium faberi is ensured to be completely immersed into the disinfectant, and the disinfection and sterilization time is 5min;
s4: preparing rooting powder, wherein the rooting powder adopts 100-200ppm of naphthylacetic acid, and after the cymbidium hyridus is disinfected and sterilized, the cymbidium hyridus is put into the rooting powder, so that the V-shaped structure at the lower end of the cymbidium hyridus is ensured to be completely put into the rooting powder, and the immersion time is 30min;
s5: preparing a culture substrate in a culture dish, wherein the culture substrate is formed by mixing 100mg/kg ATP solution and 150mg/kg NAA solution, the cymbidium faberi is dipped in 500mg/kg indoleacetic acid aqueous solution and then obliquely inserted into the culture substrate, a plastic film is covered on the surface of the culture substrate, the culture dish is placed into an incubator for culture, and the ambient temperature in the incubator is 23-27 ℃;
s6: after the root systems of the seedlings grow completely, gradually uncovering the plastic film according to days, wherein the days are 10 days, so as to prolong the illumination time of the seedlings, cutting the seedlings into a cutting bed, spraying 0.1-0.2 urea solution every 7 days, wherein the row spacing of every two adjacent seedlings is 3-5cm, the row spacing is 14-16cm, so that the upper ends of the seedlings leak out of the ground by 2-3cm, and before the seedlings are cut, punching holes at corresponding positions of the cutting bed by bamboo sticks to prevent the epidermis from being damaged and rotten in the cutting process of the seedlings;
s7: compacting soil around the seedlings, watering at proper time to ensure soil moistening, and obtaining the plum blossom plants, wherein the environment temperature of the cutting bed is 21-24 ℃, the relative humidity is 80-85%, and the shading degree is 70%.
Example 2:
the invention provides a method for rooting twigs by cutting plum blossom, which comprises the following steps:
s1: soil which is loose and fertile, good in illumination and good in drainage and air permeability is selected as an slotting bed, the requirement of plum blossom on water is high, water accumulation is not resisted, and the slotting bed is 1.5m long and 1m wide;
s2: selecting current-year semi-lignified plum blossom twigs which are 4 in the middle ten days of the month, wherein the twigs which are too tender and too lignified are poor, the twigs are too tender and easy to rot, too old auxin is reduced, the content of rooting inhibiting substances is increased, and rooting is not facilitated;
s3: preparing a disinfectant, wherein the disinfectant is formed by mixing a 10% potassium permanganate solution and 50% carbendazim, the lower end of the cymbidium faberi is put into the disinfectant for disinfection and sterilization, the V-shaped structure at the lower end of the cymbidium faberi is ensured to be completely immersed into the disinfectant, and the disinfection and sterilization time is 5min;
s4: preparing rooting powder, wherein the rooting powder adopts 100-200ppm of naphthylacetic acid, and after the cymbidium hyridus is disinfected and sterilized, the cymbidium hyridus is put into the rooting powder, so that the V-shaped structure at the lower end of the cymbidium hyridus is ensured to be completely put into the rooting powder, and the immersion time is 30min;
s5: preparing a culture substrate in a culture dish, wherein the culture substrate is formed by mixing 100mg/kg of ATP solution and 150mg/kg of IBA solution, the cymbidium faberi is dipped in 500mg/kg of indoleacetic acid aqueous solution and then obliquely inserted into the culture substrate, a plastic film is covered on the surface of the culture substrate, the culture dish is placed into an incubator for culture, and the ambient temperature in the incubator is 23-27 ℃;
s6: after the root systems of the seedlings grow completely, gradually uncovering the plastic film according to days, wherein the days are 10 days, so as to prolong the illumination time of the seedlings, cutting the seedlings into a cutting bed, spraying 0.1-0.2 urea solution every 7 days, wherein the row spacing of every two adjacent seedlings is 3-5cm, the row spacing is 14-16cm, so that the upper ends of the seedlings leak out of the ground by 2-3cm, and before the seedlings are cut, punching holes at corresponding positions of the cutting bed by bamboo sticks to prevent the epidermis from being damaged and rotten in the cutting process of the seedlings;
s7: compacting soil around the seedlings, watering at proper time to ensure that the soil is moist, and obtaining the plum blossom plants, wherein the environment temperature of the cutting bed is 21-24 ℃, the relative humidity is 80-85%, and the shading degree is 70%.
Example 3:
the invention provides a method for rooting twigs by cutting plum blossom, which comprises the following steps:
s1: soil which is loose and fertile, good in illumination and good in drainage and air permeability is selected as an slotting bed, the requirement of plum blossom on water is high, water accumulation is not resisted, and the slotting bed is 1.5m long and 1m wide;
s2: selecting current-year semi-lignified plum blossom twigs which are 4 in the middle ten days of the month, wherein the twigs which are too tender and too lignified are poor, the twigs are too tender and easy to rot, too old auxin is reduced, the content of rooting inhibiting substances is increased, and rooting is not facilitated;
s3: preparing a disinfectant, wherein the disinfectant is formed by mixing a 10% potassium permanganate solution and 50% carbendazim, and the lower end of the cymbidium faberi is put into the disinfectant for disinfection and sterilization, so that the V-shaped structure of the lower end of the cymbidium faberi is ensured to be completely immersed into the disinfectant, and the disinfection and sterilization time is 5min;
s4: preparing rooting powder, wherein the rooting powder adopts 100-200ppm of naphthylacetic acid, and after the cymbidium hyridus is sterilized, the cymbidium hyridus is put into the rooting powder, so that the lower V-shaped structure of the cymbidium hyridus is completely put into the rooting powder, and the immersion time is 30min;
s5: preparing a culture medium in a culture dish, wherein the culture medium is formed by mixing one or more of 150mg/kg NAA solution and 150mg/kg IBA solution, quickly dipping cymbidium into 500mg/kg indoleacetic acid aqueous solution, obliquely inserting the cymbidium into the culture medium, covering a plastic film on the surface of the culture medium, and putting the culture dish into an incubator for culture, wherein the ambient temperature in the incubator is 23-27 ℃;
s6: after the root systems of the seedlings grow completely, gradually uncovering the plastic film according to days, wherein the days are 10 days, so as to prolong the illumination time of the seedlings, cutting the seedlings into a cutting bed, spraying 0.1-0.2 urea solution every 7 days, wherein the row spacing of every two adjacent seedlings is 3-5cm, the row spacing is 14-16cm, so that the upper ends of the seedlings leak out of the ground by 2-3cm, and before the seedlings are cut, punching holes at corresponding positions of the cutting bed by bamboo sticks to prevent the epidermis from being damaged and rotten in the cutting process of the seedlings;
s7: compacting soil around the seedlings, watering at proper time to ensure soil moistening, and obtaining the plum blossom plants, wherein the environment temperature of the cutting bed is 21-24 ℃, the relative humidity is 80-85%, and the shading degree is 70%.
Although embodiments of the present invention have been shown and described, it will be appreciated by those skilled in the art that changes, modifications, substitutions and alterations can be made in these embodiments without departing from the principles and spirit of the invention, the scope of which is defined in the appended claims and their equivalents.
Claims (9)
1. A method for rooting a twig cutting plum blossom is characterized by comprising the following steps:
s1: selecting soil which is loose and fertile, has good illumination and good drainage and air permeability as a slotting bed, wherein the length of the slotting bed is 1.5m, and the width of the slotting bed is 1m;
s2: selecting current-year semi-grown lignified plum blossom twigs with the time of 4-5 months, selecting a twigs section with the length of 12-13cm from the upper section of the plum blossom twigs as a cymbidium hyridus, wherein the upper end of the cymbidium hyridus is a plain mouth, and the middle part of the lower end of the cymbidium hyridus is cut into a V-shaped structure, so that the shearing surface is smooth and neat;
s3: preparing a disinfectant, and putting the lower end of the cymbidium into the disinfectant for disinfection and sterilization, so as to ensure that the V-shaped structure of the lower end of the cymbidium is completely immersed into the disinfectant, wherein the disinfection and sterilization time is 5-10min;
s4: preparing rooting powder, and after the cymbidium hyridus is sterilized, putting the cymbidium hyridus into the rooting powder, and ensuring that the lower V-shaped structure of the cymbidium hyridus completely enters the rooting powder for 30min;
s5: arranging a culture medium in a culture dish, obliquely inserting cymbidium into the culture medium, covering a plastic film on the surface of the culture medium, and putting the culture dish into an incubator for culture, wherein the ambient temperature in the incubator is 23-27 ℃;
s6: after the root system of the seedling grows completely, cutting the seedling into a cutting bed, wherein the plant distance of each adjacent seedling is 3-5cm, the row distance is 14-16cm, and the upper end of the seedling leaks out of the ground by 2-3cm;
s7: compacting the soil around the seedlings, watering at proper time to ensure the soil to be moist, and obtaining the plum blossom plants.
2. The method for rooting twig-cutting plum blossom according to claim 1, characterized in that: the disinfectant is prepared by mixing a potassium permanganate solution with the concentration of 10% and carbendazim with the concentration of 50%.
3. The method for rooting twig-cutting plum blossom according to claim 1, characterized in that: the rooting powder adopts 100-200ppm of naphthylacetic acid.
4. The method for rooting twig-cutting plum blossom according to claim 1, characterized in that: the culture medium is prepared by mixing one or more of ATP solution 50-150mg/kg, NAA solution 100-150mg/kg or IBA solution 100-150 mg/kg.
5. The method for rooting twig-cutting plum blossom according to claim 1, characterized in that: in the step S5, the cymbidium is dipped into 500mg/kg of indoleacetic acid aqueous solution and then obliquely inserted into a culture medium.
6. The method for rooting twig-cutting plum blossom according to claim 1, characterized in that: in the step S6, before the seedlings are cut, holes are punched at the corresponding positions of the cutting bed by bamboo sticks, so that the epidermis is prevented from being damaged and rotten in the cutting process of the seedlings.
7. The method for rooting twig-cutting plum blossom according to claim 1, characterized in that: and in the step S6, after the root system of the seedling grows completely, gradually uncovering the plastic film for 10 days to prolong the illumination time of the seedling.
8. The method for rooting twig-cutting plum blossom according to claim 1, characterized in that: and in the step S6, spraying 0.1-0.2% of urea solution every 7-10 days after the seedlings are planted in the cutting bed.
9. The method for rooting twig-cutting plum blossom according to claim 1, characterized in that: the environment temperature of the slotting machine is 21-24 ℃, the relative humidity is 80-85%, and the shading degree is 70%.
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