CN115024106A - Double-root-cutting sleeve grafting method for medlar tomatoes - Google Patents
Double-root-cutting sleeve grafting method for medlar tomatoes Download PDFInfo
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- CN115024106A CN115024106A CN202210773633.0A CN202210773633A CN115024106A CN 115024106 A CN115024106 A CN 115024106A CN 202210773633 A CN202210773633 A CN 202210773633A CN 115024106 A CN115024106 A CN 115024106A
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- 235000007688 Lycopersicon esculentum Nutrition 0.000 title claims abstract description 60
- 244000182216 Mimusops elengi Species 0.000 title claims abstract description 51
- 235000017784 Mespilus germanica Nutrition 0.000 title claims abstract description 45
- 235000000560 Mimusops elengi Nutrition 0.000 title claims abstract description 45
- 235000007837 Vangueria infausta Nutrition 0.000 title claims abstract description 45
- 238000005520 cutting process Methods 0.000 title claims abstract description 38
- 238000000034 method Methods 0.000 title claims abstract description 24
- 240000003768 Solanum lycopersicum Species 0.000 title claims description 53
- 230000001954 sterilising effect Effects 0.000 claims abstract description 9
- 239000011159 matrix material Substances 0.000 claims abstract description 8
- 238000004321 preservation Methods 0.000 claims abstract description 8
- 235000013162 Cocos nucifera Nutrition 0.000 claims abstract description 6
- 244000060011 Cocos nucifera Species 0.000 claims abstract description 6
- 244000241872 Lycium chinense Species 0.000 claims description 40
- 235000015468 Lycium chinense Nutrition 0.000 claims description 40
- TWFZGCMQGLPBSX-UHFFFAOYSA-N Carbendazim Natural products C1=CC=C2NC(NC(=O)OC)=NC2=C1 TWFZGCMQGLPBSX-UHFFFAOYSA-N 0.000 claims description 9
- JNPZQRQPIHJYNM-UHFFFAOYSA-N carbendazim Chemical compound C1=C[CH]C2=NC(NC(=O)OC)=NC2=C1 JNPZQRQPIHJYNM-UHFFFAOYSA-N 0.000 claims description 9
- 239000006013 carbendazim Substances 0.000 claims description 9
- 239000000843 powder Substances 0.000 claims description 9
- 239000000758 substrate Substances 0.000 claims description 6
- 238000004659 sterilization and disinfection Methods 0.000 claims description 4
- DTULEIIGXRSACV-UHFFFAOYSA-N C1(=CC=CC2=CC=CC=C12)CC(=O)O.N1C(=CC2=CC=CC=C12)CCCC(=O)O Chemical compound C1(=CC=CC2=CC=CC=C12)CC(=O)O.N1C(=CC2=CC=CC=C12)CCCC(=O)O DTULEIIGXRSACV-UHFFFAOYSA-N 0.000 claims description 3
- 230000003203 everyday effect Effects 0.000 claims description 3
- 238000005507 spraying Methods 0.000 claims description 3
- 239000004563 wettable powder Substances 0.000 claims description 3
- 239000007788 liquid Substances 0.000 claims description 2
- 230000004083 survival effect Effects 0.000 abstract description 7
- 241000196324 Embryophyta Species 0.000 abstract description 5
- 239000010903 husk Substances 0.000 abstract description 5
- 241000227653 Lycopersicon Species 0.000 abstract 7
- 238000010923 batch production Methods 0.000 abstract 1
- 230000029663 wound healing Effects 0.000 description 5
- 244000241838 Lycium barbarum Species 0.000 description 4
- 235000015459 Lycium barbarum Nutrition 0.000 description 4
- 206010052428 Wound Diseases 0.000 description 4
- 208000027418 Wounds and injury Diseases 0.000 description 4
- 238000004519 manufacturing process Methods 0.000 description 4
- 230000035876 healing Effects 0.000 description 3
- 238000009396 hybridization Methods 0.000 description 3
- 235000019362 perlite Nutrition 0.000 description 2
- 239000010451 perlite Substances 0.000 description 2
- 230000035699 permeability Effects 0.000 description 2
- 235000019354 vermiculite Nutrition 0.000 description 2
- 239000010455 vermiculite Substances 0.000 description 2
- 229910052902 vermiculite Inorganic materials 0.000 description 2
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 description 1
- 241001106041 Lycium Species 0.000 description 1
- 241000208292 Solanaceae Species 0.000 description 1
- 235000002634 Solanum Nutrition 0.000 description 1
- 241000207763 Solanum Species 0.000 description 1
- 230000004075 alteration Effects 0.000 description 1
- 230000000844 anti-bacterial effect Effects 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 235000021028 berry Nutrition 0.000 description 1
- 235000013399 edible fruits Nutrition 0.000 description 1
- 239000011121 hardwood Substances 0.000 description 1
- 230000002262 irrigation Effects 0.000 description 1
- 238000003973 irrigation Methods 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 238000010008 shearing Methods 0.000 description 1
- 238000006467 substitution reaction Methods 0.000 description 1
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Classifications
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G2/00—Vegetative propagation
- A01G2/30—Grafting
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G2/00—Vegetative propagation
- A01G2/10—Vegetative propagation by means of cuttings
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G2/00—Vegetative propagation
- A01G2/30—Grafting
- A01G2/38—Holding; Ligating
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
- Y02A40/10—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in agriculture
- Y02A40/25—Greenhouse technology, e.g. cooling systems therefor
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- Life Sciences & Earth Sciences (AREA)
- Botany (AREA)
- Developmental Biology & Embryology (AREA)
- Environmental Sciences (AREA)
- Agricultural Chemicals And Associated Chemicals (AREA)
Abstract
The invention discloses a double-root-cutting sleeve grafting method for medlar and tomato, which comprises the steps of sterilizing a medlar stock and a tomato scion plant, firstly taking a fleshy stem which is germinated on a medlar seedling and is not lignified as the stock, and then taking a section of tomato seedling as a scion; keeping one leaf and axillary buds in the middle of the medlar, parallelly grafting and placing the upper end of a medlar fleshy stem and the lower end of a tomato cutting, obliquely cutting off the medlar stock and the tomato cutting at an angle of 45 degrees by using a grafting knife at the position 5-7cm above the medlar stock, sleeving a sleeve at the joint of the medlar stock and the tomato cutting, cutting a grafted seedling in a coconut husk matrix, carrying out management such as heat preservation and moisture preservation after grafting, and the like, wherein when the grafted seedling grows to 3-4 leaves after 30-35d after the cutting, the medlar root system in a hole of the hole tray becomes a lump, and the medlar grafted tomato seedling is obtained. The method adopts double-root-cutting grafting and adopts sleeve fixation, so that the cut surfaces of the stock and the scion are mutually matched, the stock and the scion are tightly contacted, the stock and the scion are not loosened and fall, the grafting survival rate is high, and the batch production is convenient.
Description
Technical Field
The invention relates to the technical field of tomato grafting, in particular to a double-root-cutting sleeve grafting method for medlar tomatoes.
Background
Grafting is one of artificial propagation methods of plants. The branch or bud of one plant is grafted to the stem or root of another plant, and the two parts which are connected together are grown into a complete plant. At present, grafting between tomatoes and medlar is difficult, although tomatoes and medlar are solanaceae plants, but different genera and two types are berries, and hybridization has certain feasibility, but the two types have great difference. The medlar belongs to the genus lycium in classification, the tomato belongs to the genus herbaceous solanum, and the hybridization between the medlar and the tomato belongs to intergeneric hybridization.
Disclosure of Invention
The present invention is directed to solving at least one of the problems of the prior art. Therefore, the invention aims to provide a double-root-cutting sleeve grafting method for medlar tomatoes.
The technical scheme of the invention is as follows: a double-root-cutting sleeve grafting method for Chinese wolfberry and tomato is characterized by comprising the following steps:
s1: watering the medlar plants and the tomato seedlings thoroughly one day before grafting, and spraying carbendazim sterilizing liquid on the leaf surfaces of the medlar plants and the tomato seedlings for sterilization;
s2: scissoring fleshy stems which germinate on perennial medlar seedlings and are not lignified as stock, wherein the length of the stock is 6-8 cm, the stock is obliquely cut from the lowest part of the fleshy stems at an angle of 45 degrees, and the stock is dipped in rooting powder solution for 10 s;
s3: taking a section of tomato seedlings as a scion by using a blade, and reserving 2-3 true leaves of the scion; removing leaves and axillary buds at the bottom end and the upper end of a Chinese wolfberry stock, keeping one leaf and axillary bud in the middle, parallelly grafting and placing the upper end of a Chinese wolfberry fleshy stem and the lower end of a tomato cutting shoot, and cutting the Chinese wolfberry stock and the tomato cutting shoot downwards at an angle of 45 degrees at the position 5-7cm away from the upper part of the Chinese wolfberry stock by using a grafting knife, wherein the length of a cut is 0.8-1.0 cm; selecting a tomato scion with proper thickness and a sleeve with a corresponding specification according to the thickness of the fleshy stem of the Chinese wolfberry stock, sleeving the sleeve at the incision of the Chinese wolfberry stock, sleeving the sleeve into the Chinese wolfberry stock 1/2, inserting the tomato scion into the sleeve, enabling the cut surfaces of the Chinese wolfberry stock and the tomato scion to be in fit and close contact with each other, and tightly sleeving the Chinese wolfberry stock and the tomato scion by the sleeve;
s4: cutting the grafted seedling in the hole of the plug tray, wherein the cutting depth is 2.5-3.0 cm, and compacting the root matrix;
s5: then quickly placing the grafted seedlings into a shed for heat preservation and moisture preservation, keeping the air humidity at 90% -95%, the daytime temperature at 25-28 ℃ and the nighttime temperature at 18-22 ℃;
s6: after three days of grafting, ventilating for 15min normally every day; after 5-7 days, the grafting wound is gradually healed, the covering film is removed and placed in the shade to prevent direct sunlight, and after 10-15 days, the medlar begins to root; when the grafted seedlings sprout new roots of 4-7 and more than 3cm, removing new buds of the medlar sprouting around the bottom of the stock of the medlar according to normal seedling management for about 15-20 days; and (3) when the leaves of the grafted seedlings grow to 3-4 leaves after 30-35 days of cuttage, and root systems of the Chinese wolfberry in the hole tray holes form lumps, so that the Chinese wolfberry grafted tomato seedlings are obtained.
Further, in step S1, the carbendazim bactericidal solution is 1000 times as much as 50% wettable powder carbendazim.
Further, in step S2, the rooting powder solution is 1000 times of solution of indolebutyric acid-naphthylacetic acid rooting powder.
Further, in step S3, the specifications of the sleeve include a 2.5mm special grafting sleeve and a 3.0mm special grafting sleeve.
Further, in step S4, the substrate is coconut husk substrate, so that the air permeability and water retention are good, and in the test, the survival rate of the medlar grafted seedling on the coconut husk substrate can reach more than 90%, which is obviously higher than the perlite substrate type of turf vermiculite.
Compared with the prior art, the invention has the following beneficial effects:
(1) by adopting a double-root-cutting grafting method, the double-root-cutting means that the tomato scions and the fleshy stems of the Chinese wolfberry are cut off, grafting is carried out, new root systems are induced to generate while healing, so that the fleshy twigs of the Chinese wolfberry subjected to cuttage have more rapid rooting compared with the hard branches, the Chinese wolfberry rooting and the healing of tomato and Chinese wolfberry grafting wounds are carried out simultaneously, the root systems are more developed, the growth speed is higher, and the survival is easier;
(2) the sleeve is adopted for fixing during grafting, so that the stock and the scion can be matched with each other and contacted closely, looseness and falling are avoided, complete wound healing is ensured, and the sleeve automatically falls off after the wound is healed completely.
(3) The method can realize mass production by utilizing the cuttage of the succulent stems of the perennial medlar, the grafting survival rate is higher than 90%, and the grafted medlar seedlings can be obtained after 30-35 days after grafting and can be used for production.
Additional aspects and advantages of the invention will be set forth in part in the description which follows and, in part, will be obvious from the description, or may be learned by practice of the invention.
Drawings
In order to more clearly illustrate the embodiments of the present invention or the technical solutions in the prior art, the drawings used in the description of the embodiments or the prior art will be briefly described below, it is obvious that the drawings in the following description are only some embodiments of the present invention, and for those skilled in the art, other drawings can be obtained according to the drawings without creative efforts:
FIG. 1 is a schematic representation of the steps of the method of the present invention;
FIG. 2 is a picture of selecting the strong fleshy roots and stems of Lycium chinense Mill;
FIG. 3 is a double-root sleeve graft picture;
FIG. 4 is a photograph of grafted wound healing;
FIG. 5 is a photograph of radication of Lycium barbarum;
FIG. 6 is a photograph of a grafted tomato seedling of Lycium barbarum;
FIG. 7 is a photograph of a field growth of a Lycium barbarum tomato;
fig. 8 is a picture of a fruit of a lycium barbarum tomato.
Detailed Description
In order to make the objects, technical solutions and advantages of the embodiments of the present invention clearer, the technical solutions in the embodiments of the present invention will be clearly and completely described below with reference to the drawings in the embodiments of the present invention, and it is obvious that the described embodiments are some, but not all, embodiments of the present invention. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.
The method for grafting the double-root-cutting sleeve of the medlar and tomato is described in detail below with reference to the attached drawings, fig. 1-8, and the method is characterized in that the tomato is used as a scion, the medlar is used as a stock, the double-root-cutting sleeve grafting method is adopted, a special grafting cutter, special grafting sleeves of 2.5mm and 3.0mm and 75% absolute ethyl alcohol are required to be prepared before grafting.
The method comprises the following steps:
s1: watering the medlar plants and the tomato seedlings thoroughly one day before grafting, and spraying carbendazim sterilizing solution to the leaf surfaces of the medlar plants and the tomato seedlings for sterilization, wherein the carbendazim sterilizing solution is 1000 times of 50% wettable powder carbendazim;
s2: the method comprises the following steps of (1) shearing fleshy stems which germinate on perennial medlar seedlings and are not lignified as stock, wherein the length of each stock is 6-8 cm, the stock is obliquely cut from the lowest part of each fleshy stem at an angle of 45 degrees, and the stock is dipped in rooting powder solution for 10s, wherein the rooting powder solution is 1000 times of indolebutyric acid-naphthylacetic acid rooting powder;
s3: taking a section of tomato seedlings as a scion by using a blade, and reserving 2-3 true leaves of the scion; removing leaves and axillary buds at the bottom end and the upper end of a Chinese wolfberry stock, keeping one leaf and the axillary buds in the middle, parallelly grafting and placing the upper end of a Chinese wolfberry succulent stem and the lower end of a tomato cutting, and obliquely cutting the Chinese wolfberry stock and the tomato cutting at an angle of 45 degrees downwards at the position 5-7cm away from the upper part of the Chinese wolfberry stock by using a grafting knife, wherein the length of a cut is 0.8-1.0 cm; selecting a tomato scion with proper thickness and a sleeve with a corresponding specification according to the thickness of the fleshy stem of the Chinese wolfberry stock, sleeving the sleeve at the incision of the Chinese wolfberry stock, sleeving the sleeve into the Chinese wolfberry stock 1/2, inserting the tomato scion into the sleeve, enabling the cut surfaces of the Chinese wolfberry stock and the tomato scion to be in fit and close contact with each other, and tightly sleeving the Chinese wolfberry stock and the tomato scion by the sleeve;
s4: cutting the grafted seedlings in the hole plate holes, wherein the cutting depth is 2.5-3.0 cm, and compacting a root matrix, wherein the matrix is a coconut husk matrix, the air permeability and water retention property are good, and in a test, the survival rate of the grafted medlar seedlings in the coconut husk matrix can reach more than 90%, and is obviously higher than the type of a turf vermiculite perlite matrix;
s5: then quickly putting the grafted seedlings into a built small arched shed for heat preservation and moisture preservation to prevent the grafted seedlings from being dehydrated and wilted, keeping the air humidity at 90-95%, the daytime temperature at 25-28 ℃ and the nighttime temperature at 18-22 ℃, and generally not needing irrigation within 7 days after grafting;
s6: after three days of grafting, ventilating for 15min normally every day; after 5-7 days, the grafting wound is gradually healed, the covering film is removed and placed in the shade to prevent direct sunlight, and after 10-15 days, the medlar begins to root; when the grafted seedlings sprout new roots of 4-7 and more than 3cm, removing new buds of the medlar sprouting around the bottom of the stock of the medlar according to normal seedling management for about 15-20 days; and (3) when the leaves of the grafted seedlings grow to 3-4 leaves after 30-35 days of cuttage, and root systems of the Chinese wolfberry in the hole tray holes form lumps, so that the Chinese wolfberry grafted tomato seedlings are obtained.
The method has the following characteristics:
(1) the double-broken-root grafting method is adopted, the double-broken root means that the root of the original stock is removed while grafting, and the new root system is induced to generate while healing, so that the purity of the grafted medlar fleshy twig seedling is higher than that of the hardwood cutting rooting, the medlar rooting and tomato medlar grafting wound healing are carried out simultaneously, the root system is developed, the growth speed is higher, and the survival is easier;
(2) the sleeve is adopted for fixation during grafting, so that the stock and the scion are in fit contact with each other, looseness and falling are avoided, complete wound healing is ensured, and the sleeve automatically falls off after the wound healing is complete. .
(3) The method can realize mass production by utilizing the cuttage of the succulent stems of the perennial medlar, the grafting survival rate is higher than 90%, and the medlar grafted seedling can be obtained after 25-30 days after grafting and is used for production.
While embodiments of the invention have been shown and described, it will be understood by those of ordinary skill in the art that: various changes, modifications, substitutions and alterations can be made to the embodiments without departing from the principles and spirit of the invention, the scope of which is defined by the claims and their equivalents.
Claims (5)
1. A double-root-cutting sleeve grafting method for Chinese wolfberry and tomato is characterized by comprising the following steps:
s1: watering the medlar plants and the tomato seedlings thoroughly one day before grafting, and spraying carbendazim sterilizing liquid on the leaf surfaces of the medlar plants and the tomato seedlings for sterilization;
s2: scissoring fleshy stems which germinate on perennial medlar seedlings and are not lignified as stock, wherein the length of the stock is 6-8 cm, the stock is obliquely cut from the lowest part of the fleshy stems at an angle of 45 degrees, and the stock is dipped in rooting powder solution for 10 s;
s3: taking a section of tomato seedlings as a scion by using a blade, and reserving 2-3 true leaves of the scion; removing leaves and axillary buds at the bottom end and the upper end of a Chinese wolfberry stock, keeping one leaf and the axillary buds in the middle, parallelly grafting and placing the upper end of a Chinese wolfberry succulent stem and the lower end of a tomato cutting, and obliquely cutting the Chinese wolfberry stock and the tomato cutting at an angle of 45 degrees downwards at the position 5-7cm away from the upper part of the Chinese wolfberry stock by using a grafting knife, wherein the length of a cut is 0.8-1.0 cm; selecting a tomato scion with proper thickness and a sleeve with a corresponding specification according to the thickness of the fleshy stem of the Chinese wolfberry stock, sleeving the sleeve at the incision of the Chinese wolfberry stock, sleeving the sleeve into the Chinese wolfberry stock 1/2, inserting the tomato scion into the sleeve, enabling the cut surfaces of the Chinese wolfberry stock and the tomato scion to be in fit and close contact with each other, and tightly sleeving the Chinese wolfberry stock and the tomato scion by the sleeve;
s4: cutting the grafted seedling in the hole of the plug tray, wherein the cutting depth is 2.5-3.0 cm, and compacting the root matrix;
s5: then, quickly placing the grafted seedlings into a shed for heat preservation and moisture preservation, keeping the air humidity at 90% -95%, the daytime temperature at 25-28 ℃ and the nighttime temperature at 18-22 ℃;
s6: after three days of grafting, ventilating for 15min normally every day; after 5-7 days, the grafting wound is gradually healed, the covering film is removed and placed in the shade to prevent direct sunlight, and after 10-15 days, the medlar begins to root; when the grafted seedlings sprout new roots of 4-7 and more than 3cm, removing new buds of the medlar sprouting around the bottom of the stock of the medlar according to normal seedling management for about 15-20 days; and (3) when the leaves of the grafted seedlings grow to 3-4 leaves after 30-35 days of cuttage, and root systems of the Chinese wolfberry in the hole tray holes form lumps, so that the Chinese wolfberry grafted tomato seedlings are obtained.
2. The double-root-cutting sleeve grafting method for the medlar tomatoes as claimed in claim 1, wherein in step S1, the carbendazim sterilization solution is 1000 times of 50% wettable powder carbendazim.
3. The double-root-cutting sleeve grafting method for the medlar tomatoes as claimed in claim 1, wherein in step S2, the rooting powder solution is 1000 times of indolebutyric acid-naphthylacetic acid rooting powder.
4. The grafting method of the double-root-cutting sleeve of the medlar tomato as claimed in claim 1, wherein in step S3, the specifications of the sleeve comprise a 2.5mm special grafting sleeve and a 3.0mm special grafting sleeve.
5. The double-root-cutting sleeve grafting method for the medlar tomatoes as claimed in claim 1, wherein in step S4, the substrate is coconut coir substrate.
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| Application Number | Priority Date | Filing Date | Title |
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| CN202210773633.0A CN115024106A (en) | 2022-07-01 | 2022-07-01 | Double-root-cutting sleeve grafting method for medlar tomatoes |
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| CN202210773633.0A CN115024106A (en) | 2022-07-01 | 2022-07-01 | Double-root-cutting sleeve grafting method for medlar tomatoes |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN115443821A (en) * | 2022-10-28 | 2022-12-09 | 宿迁市设施园艺研究院 | Intensive grafting seedling method for medlar tomatoes |
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2022
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| CN111213504A (en) * | 2019-04-11 | 2020-06-02 | 浙江大学 | Woody stock for efficient grafting of solanaceous vegetables and efficient grafting seedling method thereof |
| CN113875411A (en) * | 2021-09-14 | 2022-01-04 | 浙江大学 | Method for rapidly inducing chimeric roots of solanaceae plants by grafting technology |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN115443821A (en) * | 2022-10-28 | 2022-12-09 | 宿迁市设施园艺研究院 | Intensive grafting seedling method for medlar tomatoes |
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Application publication date: 20220909 |