CN115011670A - 通过在离散实体中条形码化对核酸进行测序 - Google Patents
通过在离散实体中条形码化对核酸进行测序 Download PDFInfo
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- CN115011670A CN115011670A CN202210828875.5A CN202210828875A CN115011670A CN 115011670 A CN115011670 A CN 115011670A CN 202210828875 A CN202210828875 A CN 202210828875A CN 115011670 A CN115011670 A CN 115011670A
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Applications Claiming Priority (4)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US201562112075P | 2015-02-04 | 2015-02-04 | |
| US62/112,075 | 2015-02-04 | ||
| CN201680020884.4A CN107530654A (zh) | 2015-02-04 | 2016-02-03 | 通过在离散实体中条形码化对核酸进行测序 |
| PCT/US2016/016444 WO2016126871A2 (en) | 2015-02-04 | 2016-02-03 | Sequencing of nucleic acids via barcoding in discrete entities |
Related Parent Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201680020884.4A Division CN107530654A (zh) | 2015-02-04 | 2016-02-03 | 通过在离散实体中条形码化对核酸进行测序 |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN115011670A true CN115011670A (zh) | 2022-09-06 |
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Family Applications (2)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202210828875.5A Pending CN115011670A (zh) | 2015-02-04 | 2016-02-03 | 通过在离散实体中条形码化对核酸进行测序 |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN120783873A (zh) * | 2025-07-09 | 2025-10-14 | 上海锦测医学检验所有限公司 | 一种智能化蛋白质组学数据异常检测与分析方法 |
Families Citing this family (237)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US8835358B2 (en) | 2009-12-15 | 2014-09-16 | Cellular Research, Inc. | Digital counting of individual molecules by stochastic attachment of diverse labels |
| CA2865575C (en) | 2012-02-27 | 2024-01-16 | Cellular Research, Inc. | Compositions and kits for molecular counting |
| WO2013177560A1 (en) | 2012-05-25 | 2013-11-28 | The Regents Of The University Of California | Microfluidic systems for particle trapping and separation |
| AU2013302867A1 (en) | 2012-08-13 | 2015-02-26 | The Regents Of The University Of California | Methods and systems for detecting biological components |
| US10584381B2 (en) | 2012-08-14 | 2020-03-10 | 10X Genomics, Inc. | Methods and systems for processing polynucleotides |
| US10323279B2 (en) | 2012-08-14 | 2019-06-18 | 10X Genomics, Inc. | Methods and systems for processing polynucleotides |
| US11591637B2 (en) | 2012-08-14 | 2023-02-28 | 10X Genomics, Inc. | Compositions and methods for sample processing |
| AU2013302756C1 (en) | 2012-08-14 | 2018-05-17 | 10X Genomics, Inc. | Microcapsule compositions and methods |
| US10273541B2 (en) | 2012-08-14 | 2019-04-30 | 10X Genomics, Inc. | Methods and systems for processing polynucleotides |
| US9701998B2 (en) | 2012-12-14 | 2017-07-11 | 10X Genomics, Inc. | Methods and systems for processing polynucleotides |
| US9951386B2 (en) | 2014-06-26 | 2018-04-24 | 10X Genomics, Inc. | Methods and systems for processing polynucleotides |
| US10221442B2 (en) | 2012-08-14 | 2019-03-05 | 10X Genomics, Inc. | Compositions and methods for sample processing |
| US10752949B2 (en) | 2012-08-14 | 2020-08-25 | 10X Genomics, Inc. | Methods and systems for processing polynucleotides |
| CA2894694C (en) | 2012-12-14 | 2023-04-25 | 10X Genomics, Inc. | Methods and systems for processing polynucleotides |
| US10533221B2 (en) | 2012-12-14 | 2020-01-14 | 10X Genomics, Inc. | Methods and systems for processing polynucleotides |
| KR20200140929A (ko) | 2013-02-08 | 2020-12-16 | 10엑스 제노믹스, 인크. | 폴리뉴클레오티드 바코드 생성 |
| EP3597772A1 (en) | 2013-04-17 | 2020-01-22 | Agency For Science, Technology And Research | Method for generating extended sequence reads |
| CA2916662C (en) | 2013-06-25 | 2022-03-08 | Prognosys Biosciences, Inc. | Methods and systems for determining spatial patterns of biological targets in a sample |
| KR102758333B1 (ko) | 2013-08-28 | 2025-01-23 | 벡톤 디킨슨 앤드 컴퍼니 | 대량의 동시 단일 세포 분석 |
| US10395758B2 (en) | 2013-08-30 | 2019-08-27 | 10X Genomics, Inc. | Sequencing methods |
| US9824068B2 (en) | 2013-12-16 | 2017-11-21 | 10X Genomics, Inc. | Methods and apparatus for sorting data |
| BR112016023625A2 (pt) | 2014-04-10 | 2018-06-26 | 10X Genomics, Inc. | dispositivos fluídicos, sistemas e métodos para encapsular e particionar reagentes, e aplicações dos mesmos |
| CA2953469A1 (en) | 2014-06-26 | 2015-12-30 | 10X Genomics, Inc. | Analysis of nucleic acid sequences |
| WO2015200893A2 (en) | 2014-06-26 | 2015-12-30 | 10X Genomics, Inc. | Methods of analyzing nucleic acids from individual cells or cell populations |
| US12312640B2 (en) | 2014-06-26 | 2025-05-27 | 10X Genomics, Inc. | Analysis of nucleic acid sequences |
| US10697007B2 (en) | 2014-06-27 | 2020-06-30 | The Regents Of The University Of California | PCR-activated sorting (PAS) |
| GB201414177D0 (en) * | 2014-08-11 | 2014-09-24 | Sphere Fluidics Ltd | Droplet Sorting |
| US10434507B2 (en) | 2014-10-22 | 2019-10-08 | The Regents Of The University Of California | High definition microdroplet printer |
| BR112017008877A2 (pt) | 2014-10-29 | 2018-07-03 | 10X Genomics Inc | métodos e composições para sequenciamento de ácido nucleico-alvo |
| US9975122B2 (en) | 2014-11-05 | 2018-05-22 | 10X Genomics, Inc. | Instrument systems for integrated sample processing |
| US10900065B2 (en) | 2014-11-14 | 2021-01-26 | University Of Washington | Methods and kits for labeling cellular molecules |
| JP6769969B2 (ja) | 2015-01-12 | 2020-10-14 | 10エックス ジェノミクス, インコーポレイテッド | 核酸配列決定ライブラリーを作製するためのプロセス及びシステム、並びにこれらを使用して作製したライブラリー |
| EP3253479B1 (en) | 2015-02-04 | 2022-09-21 | The Regents of The University of California | Sequencing of nucleic acids via barcoding in discrete entities |
| MX2017010857A (es) | 2015-02-24 | 2017-12-11 | 10X Genomics Inc | Metodos para la cobertura de secuencia de acidos nucleicos seleccionados como diana. |
| EP3262407B1 (en) | 2015-02-24 | 2023-08-30 | 10X Genomics, Inc. | Partition processing methods and systems |
| EP3901282B1 (en) | 2015-04-10 | 2023-06-28 | Spatial Transcriptomics AB | Spatially distinguished, multiplex nucleic acid analysis of biological specimens |
| AU2016253964B2 (en) * | 2015-04-27 | 2022-07-07 | Abvitro Llc | Methods of sequencing, determining, pairing, and validating therapeutic agents and disease specific antigens |
| GB201509640D0 (en) * | 2015-06-03 | 2015-07-15 | Sphere Fluidics Ltd | Systems and methods |
| JP2018525004A (ja) * | 2015-08-17 | 2018-09-06 | ザ リージェンツ オブ ザ ユニバーシティ オブ カリフォルニア | 微小液滴ベースの多置換増幅(mda)方法及び関連組成物 |
| US9862941B2 (en) | 2015-10-14 | 2018-01-09 | Pioneer Hi-Bred International, Inc. | Single cell microfluidic device |
| ES2876039T3 (es) * | 2015-11-10 | 2021-11-11 | Illumina Inc | Generación inercial de gotitas y encapsulación de partículas |
| US11371094B2 (en) | 2015-11-19 | 2022-06-28 | 10X Genomics, Inc. | Systems and methods for nucleic acid processing using degenerate nucleotides |
| JP6954899B2 (ja) | 2015-12-04 | 2021-10-27 | 10エックス ゲノミクス,インコーポレイテッド | 核酸の解析のための方法及び組成物 |
| WO2017138984A1 (en) | 2016-02-11 | 2017-08-17 | 10X Genomics, Inc. | Systems, methods, and media for de novo assembly of whole genome sequence data |
| WO2017181161A1 (en) | 2016-04-15 | 2017-10-19 | Predicine, Inc. | Systems and methods for detecting genetic alterations |
| WO2018183796A1 (en) * | 2017-03-31 | 2018-10-04 | Predicine, Inc. | Systems and methods for predicting and monitoring cancer therapy |
| US20190127789A1 (en) | 2016-04-19 | 2019-05-02 | President And Fellows Of Harvard College | Immobilization-based systems and methods for genetic analysis and other applications |
| WO2017197343A2 (en) | 2016-05-12 | 2017-11-16 | 10X Genomics, Inc. | Microfluidic on-chip filters |
| WO2017197338A1 (en) | 2016-05-13 | 2017-11-16 | 10X Genomics, Inc. | Microfluidic systems and methods of use |
| US10202641B2 (en) | 2016-05-31 | 2019-02-12 | Cellular Research, Inc. | Error correction in amplification of samples |
| CN109563539A (zh) | 2016-06-15 | 2019-04-02 | 慕尼黑路德维希马克西米利安斯大学 | 使用dna纳米技术的单分子检测或定量 |
| US10830545B2 (en) | 2016-07-12 | 2020-11-10 | Fractal Heatsink Technologies, LLC | System and method for maintaining efficiency of a heat sink |
| JP6853523B2 (ja) * | 2016-07-27 | 2021-03-31 | 国立大学法人京都大学 | ヘリカーゼを用いたpcr |
| EP3497228A4 (en) | 2016-08-10 | 2020-05-27 | The Regents of The University of California | MULTI-DISPLACEMENT AMPLIFICATION AND PCR COMBINED IN AN EMULSION MICRO DROPLET |
| WO2018053119A2 (en) | 2016-09-15 | 2018-03-22 | Augmenta Bioworks, Inc. | Immune repertoire sequence amplification methods and applications |
| US11174503B2 (en) | 2016-09-21 | 2021-11-16 | Predicine, Inc. | Systems and methods for combined detection of genetic alterations |
| CN109791157B (zh) | 2016-09-26 | 2022-06-07 | 贝克顿迪金森公司 | 使用具有条形码化的寡核苷酸序列的试剂测量蛋白质表达 |
| WO2018067792A1 (en) * | 2016-10-07 | 2018-04-12 | President And Fellows Of Harvard College | Sequencing of bacteria or other species |
| US11090653B2 (en) | 2016-10-11 | 2021-08-17 | The Regents Of The University Of California | Systems and methods to encapsulate and preserve organic matter for analysis |
| CA3032613A1 (en) | 2016-11-10 | 2018-05-17 | Takara Bio Usa, Inc. | Methods of producing amplified double stranded deoxyribonucleic acids and compositions and kits for use therein |
| GB2563105B (en) * | 2016-11-16 | 2022-10-19 | Catalog Tech Inc | Nucleic acid-based data storage |
| CN110114473A (zh) * | 2016-11-23 | 2019-08-09 | 斯特拉斯堡大学 | 靶分子的串联条形码添加以便以单实体分辨率对靶分子进行绝对定量 |
| CA3047328A1 (en) | 2016-12-21 | 2018-06-28 | The Regents Of The University Of California | Single cell genomic sequencing using hydrogel based droplets |
| US10815525B2 (en) | 2016-12-22 | 2020-10-27 | 10X Genomics, Inc. | Methods and systems for processing polynucleotides |
| US10011872B1 (en) | 2016-12-22 | 2018-07-03 | 10X Genomics, Inc. | Methods and systems for processing polynucleotides |
| US10550429B2 (en) | 2016-12-22 | 2020-02-04 | 10X Genomics, Inc. | Methods and systems for processing polynucleotides |
| US12264411B2 (en) | 2017-01-30 | 2025-04-01 | 10X Genomics, Inc. | Methods and systems for analysis |
| EP4029939B1 (en) | 2017-01-30 | 2023-06-28 | 10X Genomics, Inc. | Methods and systems for droplet-based single cell barcoding |
| US10995333B2 (en) | 2017-02-06 | 2021-05-04 | 10X Genomics, Inc. | Systems and methods for nucleic acid preparation |
| EP3838268B1 (en) * | 2017-02-24 | 2023-05-10 | The Regents of the University of California | Particle-drop structures and methods for making and using the same |
| CN107083423B (zh) * | 2017-03-27 | 2022-01-28 | 北京极客基因科技有限公司 | 一种药物靶点预测和药物全方面评价方法 |
| US12054764B2 (en) | 2017-04-14 | 2024-08-06 | The Broad Institute, Inc. | High-throughput screens for exploring biological functions of microscale biological systems |
| US10544413B2 (en) | 2017-05-18 | 2020-01-28 | 10X Genomics, Inc. | Methods and systems for sorting droplets and beads |
| EP4435113B1 (en) | 2017-05-18 | 2025-12-10 | 10X Genomics, Inc. | Methods and systems for sorting droplets and beads |
| KR102550778B1 (ko) * | 2017-05-26 | 2023-07-03 | 에이비비트로 엘엘씨 | 고-처리량 폴리뉴클레오타이드 라이브러리 시퀀싱 및 전사체 분석 |
| US10400235B2 (en) | 2017-05-26 | 2019-09-03 | 10X Genomics, Inc. | Single cell analysis of transposase accessible chromatin |
| EP3445876B1 (en) | 2017-05-26 | 2023-07-05 | 10X Genomics, Inc. | Single cell analysis of transposase accessible chromatin |
| CN110719959B (zh) | 2017-06-05 | 2021-08-06 | 贝克顿迪金森公司 | 针对单细胞的样品索引 |
| US11517901B2 (en) | 2017-06-09 | 2022-12-06 | The Regents Of The University Of California | High-efficiency particle encapsulation in droplets with particle spacing and downstream droplet sorting |
| WO2018227210A1 (en) | 2017-06-09 | 2018-12-13 | The Regents Of The University Of California | High-efficiency encapsulation in droplets based on hydrodynamic vortices control |
| US11186862B2 (en) | 2017-06-20 | 2021-11-30 | Bio-Rad Laboratories, Inc. | MDA using bead oligonucleotide |
| TWI637834B (zh) * | 2017-07-11 | 2018-10-11 | 台灣奈米碳素股份有限公司 | 微流道裝置的製造方法及其結構 |
| CN111433549A (zh) | 2017-07-17 | 2020-07-17 | 分形散热器技术有限责任公司 | 多重分形散热器系统及方法 |
| GB2581599B8 (en) * | 2017-08-10 | 2023-09-20 | Element Biosciences Inc | Tagging nucleic acid molecules from single cells for phased sequencing |
| US10610865B2 (en) | 2017-08-22 | 2020-04-07 | 10X Genomics, Inc. | Droplet forming devices and system with differential surface properties |
| ES2994431T3 (en) | 2017-09-22 | 2025-01-23 | Univ Washington | In situ combinatorial labeling of cellular molecules |
| EP4462123A3 (en) | 2017-09-27 | 2025-02-12 | Bio-Rad Laboratories, Inc. | Digital affinity linkage assay |
| WO2019139650A2 (en) | 2017-09-29 | 2019-07-18 | The Regents Of The University Of California | Method of generating monodisperse emulsions |
| US10837047B2 (en) | 2017-10-04 | 2020-11-17 | 10X Genomics, Inc. | Compositions, methods, and systems for bead formation using improved polymers |
| WO2019075409A1 (en) | 2017-10-12 | 2019-04-18 | The Regents Of The University Of California | ISOLATION AND IDENTIFICATION WITHOUT MICROFLUIDIC LABEL OF CELLS USING FLUORESCENCE LIFE IMAGING (FLIM) |
| US10501739B2 (en) | 2017-10-18 | 2019-12-10 | Mission Bio, Inc. | Method, systems and apparatus for single cell analysis |
| WO2019079640A1 (en) * | 2017-10-18 | 2019-04-25 | Mission Bio, Inc. | METHOD, SYSTEMS, AND DEVICE FOR HIGH-EFFICIENCY UNICELLULAR DNA SEQUENCING OF DROPLET MICROFLUIDS |
| US11499127B2 (en) | 2017-10-20 | 2022-11-15 | The Regents Of The University Of California | Multi-layered microfluidic systems for in vitro large-scale perfused capillary networks |
| WO2019079787A1 (en) | 2017-10-20 | 2019-04-25 | The Regents Of The University Of California | MICROFLUIDIC SYSTEMS AND METHODS FOR CELL TRANSFECTION VIA LIPOFLEX |
| WO2019084055A1 (en) * | 2017-10-23 | 2019-05-02 | Massachusetts Institute Of Technology | CLASSIFICATION OF GENETIC VARIATION FROM UNICELLULAR TRANSCRIPTOMS |
| WO2019083852A1 (en) | 2017-10-26 | 2019-05-02 | 10X Genomics, Inc. | MICROFLUIDIC CHANNEL NETWORKS FOR PARTITIONING |
| WO2019084043A1 (en) | 2017-10-26 | 2019-05-02 | 10X Genomics, Inc. | METHODS AND SYSTEMS FOR NUCLEIC ACID PREPARATION AND CHROMATIN ANALYSIS |
| EP3700672B1 (en) | 2017-10-27 | 2022-12-28 | 10X Genomics, Inc. | Methods for sample preparation and analysis |
| SG11201913654QA (en) | 2017-11-15 | 2020-01-30 | 10X Genomics Inc | Functionalized gel beads |
| US10829815B2 (en) | 2017-11-17 | 2020-11-10 | 10X Genomics, Inc. | Methods and systems for associating physical and genetic properties of biological particles |
| EP3717661A1 (en) | 2017-11-27 | 2020-10-07 | The Trustees of Columbia University in the City of New York | Rna printing and sequencing devices, methods, and systems |
| WO2019108851A1 (en) | 2017-11-30 | 2019-06-06 | 10X Genomics, Inc. | Systems and methods for nucleic acid preparation and analysis |
| WO2019113235A1 (en) * | 2017-12-06 | 2019-06-13 | 10X Genomics, Inc. | Methods and systems for processing nucleic acid molecules |
| EP3724658A1 (en) * | 2017-12-12 | 2020-10-21 | 10X Genomics, Inc. | Systems and methods for single cell processing |
| US20200392562A1 (en) * | 2017-12-22 | 2020-12-17 | Thermo Fisher Scientific Baltics Uab | Polymerase chain reaction composition comprising amines |
| CN111712579B (zh) | 2017-12-22 | 2024-10-15 | 10X基因组学有限公司 | 用于处理来自一个或多个细胞的核酸分子的系统和方法 |
| ES2937927T3 (es) | 2018-01-29 | 2023-04-03 | St Jude Childrens Res Hospital Inc | Método para la amplificación de ácidos nucleicos |
| SG11202007686VA (en) | 2018-02-12 | 2020-09-29 | 10X Genomics Inc | Methods characterizing multiple analytes from individual cells or cell populations |
| US11639928B2 (en) | 2018-02-22 | 2023-05-02 | 10X Genomics, Inc. | Methods and systems for characterizing analytes from individual cells or cell populations |
| WO2019169028A1 (en) * | 2018-02-28 | 2019-09-06 | 10X Genomics, Inc. | Transcriptome sequencing through random ligation |
| EP3759213B1 (en) * | 2018-03-02 | 2026-04-29 | Quantumcyte, Inc. | Methods, compositions, and devices for isolation and expression analysis of regions of interest from a tissue |
| EP3762494B1 (en) | 2018-03-07 | 2022-11-23 | Wisconsin Alumni Research Foundation | High throughput nucleic acid profiling of single cells |
| CN112262218B (zh) | 2018-04-06 | 2024-11-08 | 10X基因组学有限公司 | 用于单细胞处理中的质量控制的系统和方法 |
| SG11201911961RA (en) | 2018-04-20 | 2020-01-30 | Illumina Inc | Methods of encapsulating single cells, the encapsulated cells and uses thereof |
| CN112272710A (zh) | 2018-05-03 | 2021-01-26 | 贝克顿迪金森公司 | 高通量多组学样品分析 |
| WO2019217758A1 (en) | 2018-05-10 | 2019-11-14 | 10X Genomics, Inc. | Methods and systems for molecular library generation |
| EP3797162A1 (en) * | 2018-05-22 | 2021-03-31 | Université de Strasbourg | Absolute quantification of target molecules at single-entity resolution using tandem barcoding |
| US11932899B2 (en) | 2018-06-07 | 2024-03-19 | 10X Genomics, Inc. | Methods and systems for characterizing nucleic acid molecules |
| US11703427B2 (en) | 2018-06-25 | 2023-07-18 | 10X Genomics, Inc. | Methods and systems for cell and bead processing |
| WO2020009998A1 (en) * | 2018-07-03 | 2020-01-09 | Chan Zuckerberg Biohub, Inc. | Method to perform high-throughput single cell genomic and phenotypic analyses |
| WO2020010258A1 (en) * | 2018-07-05 | 2020-01-09 | Accuragen Holdings Limited | Compositions and methods for digital polymerase chain reaction |
| US12188014B1 (en) | 2018-07-25 | 2025-01-07 | 10X Genomics, Inc. | Compositions and methods for nucleic acid processing using blocking agents |
| US20200032335A1 (en) | 2018-07-27 | 2020-01-30 | 10X Genomics, Inc. | Systems and methods for metabolome analysis |
| SG11202101164TA (en) | 2018-08-03 | 2021-03-30 | 10X Genomics Inc | Methods and systems to minimize barcode exchange |
| AU2019315604B2 (en) | 2018-08-03 | 2025-04-17 | Catolog Technologies, Inc | Systems and methods for storing and reading nucleic acid-based data with error protection |
| US20200048626A1 (en) * | 2018-08-07 | 2020-02-13 | Augmenta Bioworks, Inc. | Methods for Making Gel Beads and Core and Shell Beads with a Cell |
| US20210301247A1 (en) * | 2018-08-10 | 2021-09-30 | Cellino Biotech, Inc. | System for image-driven cell manufacturing |
| AU2019321552A1 (en) | 2018-08-17 | 2021-03-11 | The Regents Of The University Of California | Monodispersed particle-triggered droplet formation from stable jets |
| US12065688B2 (en) * | 2018-08-20 | 2024-08-20 | 10X Genomics, Inc. | Compositions and methods for cellular processing |
| WO2020041148A1 (en) | 2018-08-20 | 2020-02-27 | 10X Genomics, Inc. | Methods and systems for detection of protein-dna interactions using proximity ligation |
| ES2992135T3 (es) | 2018-10-01 | 2024-12-09 | Becton Dickinson Co | Determinar secuencias de transcripción 5 |
| WO2020077017A2 (en) | 2018-10-10 | 2020-04-16 | Augmenta Bioworks, Inc. | Methods for isolating immune binding proteins |
| US11608524B1 (en) * | 2018-10-25 | 2023-03-21 | Wisconsin Alumni Research Foundation | Methods of analyzing cells |
| WO2020086843A1 (en) * | 2018-10-26 | 2020-04-30 | Illumina, Inc. | Modulating polymer beads for dna processing |
| US11459607B1 (en) | 2018-12-10 | 2022-10-04 | 10X Genomics, Inc. | Systems and methods for processing-nucleic acid molecules from a single cell using sequential co-partitioning and composite barcodes |
| WO2020123316A2 (en) * | 2018-12-10 | 2020-06-18 | 10X Genomics, Inc. | Methods for determining a location of a biological analyte in a biological sample |
| SG11202105441WA (en) | 2018-12-13 | 2021-06-29 | Dna Script | Direct oligonucleotide synthesis on cells and biomolecules |
| TWI725686B (zh) | 2018-12-26 | 2021-04-21 | 財團法人工業技術研究院 | 用於產生液珠的管狀結構及液珠產生方法 |
| US20220033811A1 (en) * | 2018-12-28 | 2022-02-03 | Biobloxx Ab | Method and kit for preparing complementary dna |
| EP3906318A1 (en) * | 2019-01-06 | 2021-11-10 | 10X Genomics, Inc. | Methods and systems for enrichment of barcodes |
| US12169198B2 (en) | 2019-01-08 | 2024-12-17 | 10X Genomics, Inc. | Systems and methods for sample analysis |
| US11845983B1 (en) | 2019-01-09 | 2023-12-19 | 10X Genomics, Inc. | Methods and systems for multiplexing of droplet based assays |
| WO2020154391A1 (en) * | 2019-01-22 | 2020-07-30 | Mission Bio | Methods of nucleic acid detection and primer design |
| EP3924505B1 (en) | 2019-02-12 | 2025-12-17 | 10X Genomics, Inc. | Methods for processing nucleic acid molecules |
| US12305239B2 (en) | 2019-02-12 | 2025-05-20 | 10X Genomics, Inc. | Analysis of nucleic acid sequences |
| US12275993B2 (en) | 2019-02-12 | 2025-04-15 | 10X Genomics, Inc. | Analysis of nucleic acid sequences |
| WO2020167866A1 (en) | 2019-02-12 | 2020-08-20 | 10X Genomics, Inc. | Systems and methods for transposon loading |
| US11467153B2 (en) | 2019-02-12 | 2022-10-11 | 10X Genomics, Inc. | Methods for processing nucleic acid molecules |
| US11851683B1 (en) | 2019-02-12 | 2023-12-26 | 10X Genomics, Inc. | Methods and systems for selective analysis of cellular samples |
| WO2020167862A1 (en) | 2019-02-12 | 2020-08-20 | 10X Genomics, Inc. | Systems and methods for transfer of reagents between droplets |
| WO2020167920A1 (en) | 2019-02-14 | 2020-08-20 | Cellular Research, Inc. | Hybrid targeted and whole transcriptome amplification |
| US11655499B1 (en) | 2019-02-25 | 2023-05-23 | 10X Genomics, Inc. | Detection of sequence elements in nucleic acid molecules |
| SG11202111242PA (en) | 2019-03-11 | 2021-11-29 | 10X Genomics Inc | Systems and methods for processing optically tagged beads |
| WO2020183591A1 (en) * | 2019-03-11 | 2020-09-17 | Enplas Corporation | Fluid handling method, and fluid handling device and fluid handling system used for the method |
| EP3947722A1 (en) | 2019-03-27 | 2022-02-09 | 10X Genomics, Inc. | Systems and methods for processing rna from cells |
| CA3132029A1 (en) * | 2019-04-02 | 2020-10-08 | Dalia Dhingra | Methods and systems for proteomic profiling and characterization |
| CA3132030A1 (en) * | 2019-04-02 | 2020-10-08 | Dalia Dhingra | Methods, systems, and apparatus for nucleic acid detection |
| AU2020280104A1 (en) | 2019-05-22 | 2022-01-20 | Mission Bio, Inc. | Method and apparatus for simultaneous targeted sequencing of DNA, RNA and protein |
| CN114585751A (zh) * | 2019-06-12 | 2022-06-03 | 阿尔缇玛基因组学公司 | 使用分子条形码进行准确碱基判定的方法 |
| CA3145243A1 (en) * | 2019-06-27 | 2020-12-30 | Cell Microsystems, Inc. | Systems and methods for associating single cell imaging with rna transcriptomics |
| US12186751B2 (en) | 2019-06-28 | 2025-01-07 | 10X Genomics, Inc. | Devices and systems incorporating acoustic ordering and methods of use thereof |
| US11667954B2 (en) | 2019-07-01 | 2023-06-06 | Mission Bio, Inc. | Method and apparatus to normalize quantitative readouts in single-cell experiments |
| CN113891961A (zh) * | 2019-07-12 | 2022-01-04 | 深圳华大生命科学研究院 | 一种全基因组全流程微流控自动化建库方法和装置 |
| CN120099137A (zh) | 2019-07-22 | 2025-06-06 | 贝克顿迪金森公司 | 单细胞染色质免疫沉淀测序测定 |
| MX2022001324A (es) * | 2019-07-31 | 2022-05-19 | Bioskryb Genomics Inc | Análisis de células individuales. |
| US20220282326A1 (en) * | 2019-08-05 | 2022-09-08 | Mission Bio, Inc. | Method and Apparatus for Single-Cell Analysis for Determining a Cell Trajectory |
| US12179199B2 (en) | 2019-08-09 | 2024-12-31 | The Regents Of The University Of California | Microfluidic single-cell pairing array for studying cell-cell interactions in isolated compartments |
| CN115298543A (zh) * | 2019-09-04 | 2022-11-04 | 范德比尔特大学 | 抗原抗体特异性结合识别方法 |
| US12235262B1 (en) | 2019-09-09 | 2025-02-25 | 10X Genomics, Inc. | Methods and systems for single cell protein analysis |
| US20220339623A1 (en) * | 2019-09-30 | 2022-10-27 | Frederick Edward TURNER | Devices for determining a target nucleic acid profile and methods of use thereof |
| JP2022550596A (ja) * | 2019-10-05 | 2022-12-02 | ミッション バイオ インコーポレイテッド | シングルセルで同時に検出されるコピー数変動及び単一ヌクレオチド変動のための方法、システム、及び装置 |
| WO2021072306A1 (en) | 2019-10-10 | 2021-04-15 | 1859, Inc. | Methods and systems for microfluidic screening |
| WO2021076841A1 (en) * | 2019-10-16 | 2021-04-22 | Georgia Tech Research Corporation | Methods for ultrasensitive detection of protein and cellular biomarkers |
| CN110777048A (zh) * | 2019-11-01 | 2020-02-11 | 上海市第六人民医院 | 一种高通量单外泌体分离装置 |
| JP7522189B2 (ja) | 2019-11-08 | 2024-07-24 | ベクトン・ディキンソン・アンド・カンパニー | 免疫レパートリーシーケンシングのための完全長v(d)j情報を得るためのランダムプライミングの使用 |
| US20220364147A1 (en) * | 2019-11-15 | 2022-11-17 | Miltenyi Biotec B.V. & Co. KG | Color and bardcoded beads for single cell indexing |
| US12059679B2 (en) * | 2019-11-19 | 2024-08-13 | 10X Genomics, Inc. | Methods and devices for sorting droplets and particles |
| US12163189B2 (en) | 2019-11-27 | 2024-12-10 | University Of Washington | Method for preparation and high-throughput microbial single-cell RNA sequencing of bacteria |
| GB201918340D0 (en) * | 2019-12-12 | 2020-01-29 | Cambridge Entpr Ltd | Spatial barcoding |
| EP4081656A1 (en) | 2019-12-23 | 2022-11-02 | 10X Genomics, Inc. | Compositions and methods for using fixed biological samples in partition-based assays |
| CN115135984B (zh) | 2019-12-23 | 2025-12-23 | 10X基因组学有限公司 | 可逆固定试剂及其使用方法 |
| WO2021128035A1 (zh) * | 2019-12-25 | 2021-07-01 | 苏州绘真生物科技有限公司 | 高通量单细胞转录组测序方法及试剂盒 |
| US11649497B2 (en) | 2020-01-13 | 2023-05-16 | Becton, Dickinson And Company | Methods and compositions for quantitation of proteins and RNA |
| CN115698282A (zh) | 2020-01-13 | 2023-02-03 | 福路伦特生物科学公司 | 单细胞测序 |
| WO2021146219A1 (en) | 2020-01-13 | 2021-07-22 | Becton, Dickinson And Company | Cell capture using du-containing oligonucleotides |
| WO2021146187A1 (en) | 2020-01-13 | 2021-07-22 | Fluent Biosciences Inc. | Emulsion based drug screening |
| JP7730449B2 (ja) | 2020-01-13 | 2025-08-28 | イルミナ インコーポレイテッド | 単一細胞遺伝子プロファイリングのための方法およびシステム |
| US20230045126A1 (en) * | 2020-01-14 | 2023-02-09 | President And Fellows Of Harvard College | Devices and methods for determining nucleic acids using digital droplet pcr and related techniques |
| US11732299B2 (en) | 2020-01-21 | 2023-08-22 | 10X Genomics, Inc. | Spatial assays with perturbed cells |
| EP4095256A4 (en) * | 2020-01-23 | 2023-03-15 | DGI Tech (Qing Dao) Co., Limited | DROPLET MICROFLUIDIC-BASED SINGLE-CELL SEQUENCE AND APPLICATIONS |
| EP4093884A4 (en) | 2020-01-24 | 2024-05-15 | Vanderbilt University | Methods for identification of ligand-blocking antibodies and for determining antibody potency |
| US20210230681A1 (en) | 2020-01-24 | 2021-07-29 | 10X Genomics, Inc. | Methods for spatial analysis using proximity ligation |
| WO2021155057A1 (en) | 2020-01-29 | 2021-08-05 | Becton, Dickinson And Company | Barcoded wells for spatial mapping of single cells through sequencing |
| US11835462B2 (en) | 2020-02-11 | 2023-12-05 | 10X Genomics, Inc. | Methods and compositions for partitioning a biological sample |
| US12449419B1 (en) | 2020-02-12 | 2025-10-21 | 10X Genomics, Inc. | Methods for detecting binding of peptide-MHC monomers to T cells |
| WO2021173719A1 (en) | 2020-02-25 | 2021-09-02 | Becton, Dickinson And Company | Bi-specific probes to enable the use of single-cell samples as single color compensation control |
| US11926863B1 (en) | 2020-02-27 | 2024-03-12 | 10X Genomics, Inc. | Solid state single cell method for analyzing fixed biological cells |
| CN111454942A (zh) * | 2020-03-16 | 2020-07-28 | 张晓鲁 | 一种同一样品的转录组和基因组同时建立测序文库的构建方法 |
| US11866782B2 (en) | 2020-03-16 | 2024-01-09 | Fluent Biosciences Inc. | Multi-omic analysis in monodisperse droplets |
| AU2021240092A1 (en) * | 2020-03-20 | 2022-10-27 | Mission Bio, Inc. | Single cell workflow for whole genome amplification |
| WO2021195169A1 (en) | 2020-03-24 | 2021-09-30 | Fluent Biosciences Inc. | Viral detection using template emulsification |
| CN111394432B (zh) * | 2020-03-27 | 2023-03-24 | 深圳闪量科技有限公司 | 基于通用探针芯片的多重定量pcr检测系统 |
| CN115997030A (zh) * | 2020-04-30 | 2023-04-21 | 德迈逊科技有限公司 | 用于大分子操作的装置和方法 |
| GB202007059D0 (en) * | 2020-05-13 | 2020-06-24 | Univ Oxford Innovation Ltd | Polynucleotide arrays |
| US11851700B1 (en) | 2020-05-13 | 2023-12-26 | 10X Genomics, Inc. | Methods, kits, and compositions for processing extracellular molecules |
| US11661625B2 (en) | 2020-05-14 | 2023-05-30 | Becton, Dickinson And Company | Primers for immune repertoire profiling |
| EP4414459B1 (en) | 2020-05-22 | 2025-09-03 | 10X Genomics, Inc. | Simultaneous spatio-temporal measurement of gene expression and cellular activity |
| CN115867676A (zh) * | 2020-05-29 | 2023-03-28 | 国立研究开发法人理化学研究所 | 细胞群的处理方法和细胞群中所含的基因的分析方法 |
| US12157913B2 (en) | 2020-06-02 | 2024-12-03 | Becton, Dickinson And Company | Oligonucleotides and beads for 5 prime gene expression assay |
| US12265079B1 (en) | 2020-06-02 | 2025-04-01 | 10X Genomics, Inc. | Systems and methods for detecting analytes from captured single biological particles |
| US11932901B2 (en) | 2020-07-13 | 2024-03-19 | Becton, Dickinson And Company | Target enrichment using nucleic acid probes for scRNAseq |
| EP4182477A4 (en) | 2020-07-15 | 2024-09-11 | Fluent Biosciences Inc. | MULTI-LEVEL LIGATION OLIGOS |
| US12391940B2 (en) | 2020-07-31 | 2025-08-19 | Becton, Dickinson And Company | Single cell assay for transposase-accessible chromatin |
| CN114073995B (zh) * | 2020-08-13 | 2026-03-17 | 安行生物技术有限公司 | 分析单细胞的微流控设备和单元及其方法 |
| GB202012730D0 (en) * | 2020-08-14 | 2020-09-30 | Cambridge Entpr Ltd | Modular microfluidic devices, systems and methodds for total RNA analyses |
| US12480158B1 (en) | 2020-11-05 | 2025-11-25 | 10X Genomics, Inc. | Methods and systems for processing polynucleotides |
| US12084715B1 (en) | 2020-11-05 | 2024-09-10 | 10X Genomics, Inc. | Methods and systems for reducing artifactual antisense products |
| WO2022099208A1 (en) * | 2020-11-09 | 2022-05-12 | The General Hospital Corporation | High throughput microfluidics for analysis of immune cell activation |
| WO2022103712A1 (en) * | 2020-11-13 | 2022-05-19 | 10X Genomics, Inc. | Nano-partitions (encapsulated nucleic acid processing enzymes) for cell-lysis and multiple reactions in partition-based assays |
| EP4247967A1 (en) | 2020-11-20 | 2023-09-27 | Becton, Dickinson and Company | Profiling of highly expressed and lowly expressed proteins |
| US20220243264A1 (en) * | 2020-11-23 | 2022-08-04 | The Broad Institute, Inc. | Systems and methods for amplifying rna |
| CN116685850A (zh) | 2020-12-15 | 2023-09-01 | 贝克顿迪金森公司 | 单细胞分泌组分析 |
| CN113322254B (zh) * | 2021-01-06 | 2022-05-20 | 南京诺唯赞生物科技股份有限公司 | 多靶点蛋白质-dna相互作用的研究方法和工具 |
| US12398262B1 (en) | 2021-01-22 | 2025-08-26 | 10X Genomics, Inc. | Triblock copolymer-based cell stabilization and fixation system and methods of use thereof |
| CN114836838A (zh) * | 2021-02-01 | 2022-08-02 | 南方医科大学 | 一种中通量单细胞拷贝数文库构建的方法及其应用 |
| CN117015617B (zh) | 2021-02-23 | 2025-04-04 | 10X基因组学有限公司 | 基于探针的核酸和蛋白质分析 |
| WO2022221391A1 (en) | 2021-04-14 | 2022-10-20 | Partillion Bioscience Corporation | Nanoscale reaction chambers and methods of using the same |
| EP4330678A1 (en) * | 2021-04-26 | 2024-03-06 | Ecole Polytechnique Federale De Lausanne (Epfl) | High complexity microcompartment-based interaction screening |
| WO2023044307A1 (en) * | 2021-09-14 | 2023-03-23 | Becton, Dickinson And Company | Full length single cell rna sequencing |
| WO2023154816A1 (en) * | 2022-02-09 | 2023-08-17 | Mission Bio, Inc. | Systems and methods of detecting merged droplets in single cell sequencing |
| DK181862B1 (en) * | 2022-03-21 | 2025-02-24 | Samplix Aps | Method and kit for targeted enrichment of large dna molecules for long-read sequencing using facs or microfluidic partitioning |
| WO2023212223A1 (en) * | 2022-04-28 | 2023-11-02 | BioSkryb Genomics, Inc. | Single cell multiomics |
| EP4540607A1 (en) | 2022-06-17 | 2025-04-23 | 10X Genomics, Inc. | Catalytic de-crosslinking of samples for in situ analysis |
| US12534721B2 (en) | 2022-10-06 | 2026-01-27 | Illumina, Inc. | Quantitative detection and analysis of moleculesip |
| WO2024097393A1 (en) * | 2022-11-04 | 2024-05-10 | Fluid Discovery | Systems, compositions, and methods for single cell analysis |
| KR20250137709A (ko) * | 2023-01-30 | 2025-09-18 | 아이덱스 래보러토리즈, 인코포레이티드 | 전기습윤 바코드 자성 비드를 위한 방법, 시스템 및 디바이스 |
| WO2025040497A1 (en) * | 2023-08-18 | 2025-02-27 | Qiagen Gmbh | Method for improving nucleic acid processing |
Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101432439A (zh) * | 2006-02-24 | 2009-05-13 | 考利达基因组股份有限公司 | Dna阵列上的高通量基因组测序 |
| US20110059556A1 (en) * | 2009-09-04 | 2011-03-10 | The Research Foundation Of State University Of New York | Rapid and Continuous Analyte Processing in Droplet Microfluidic Devices |
| WO2012109600A2 (en) * | 2011-02-11 | 2012-08-16 | Raindance Technologies, Inc. | Methods for forming mixed droplets |
| US20120220494A1 (en) * | 2011-02-18 | 2012-08-30 | Raindance Technolgies, Inc. | Compositions and methods for molecular labeling |
| US20140323316A1 (en) * | 2013-03-15 | 2014-10-30 | Complete Genomics, Inc. | Multiple tagging of individual long dna fragments |
| US20140378349A1 (en) * | 2012-08-14 | 2014-12-25 | 10X Technologies, Inc. | Compositions and methods for sample processing |
Family Cites Families (117)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP0680335A1 (en) | 1992-11-30 | 1995-11-08 | The Wellcome Foundation Limited | Sequential targeting of tumor sites with oligonucleotide conjugates of antibody and complementary oligonucleotide conjugates of chelated radionuclide |
| US20020068357A1 (en) | 1995-09-28 | 2002-06-06 | Mathies Richard A. | Miniaturized integrated nucleic acid processing and analysis device and method |
| US6143496A (en) | 1997-04-17 | 2000-11-07 | Cytonix Corporation | Method of sampling, amplifying and quantifying segment of nucleic acid, polymerase chain reaction assembly having nanoliter-sized sample chambers, and method of filling assembly |
| US6319507B1 (en) | 1997-05-02 | 2001-11-20 | Kobo Products, Inc. | Agar gel bead composition and method |
| ES2230701T3 (es) | 1997-07-07 | 2005-05-01 | Medical Research Council | Procedimiento de seleccion in vitro. |
| US6436677B1 (en) | 2000-03-02 | 2002-08-20 | Promega Corporation | Method of reverse transcription |
| WO2003054524A1 (en) | 2001-12-11 | 2003-07-03 | Sau Lan Tang Staats | Microfluidic devices and methods for two-dimensional separations |
| JP2006507921A (ja) | 2002-06-28 | 2006-03-09 | プレジデント・アンド・フェロウズ・オブ・ハーバード・カレッジ | 流体分散のための方法および装置 |
| CA2513899C (en) | 2003-01-29 | 2013-03-26 | 454 Corporation | Methods of amplifying and sequencing nucleic acids |
| US7041481B2 (en) | 2003-03-14 | 2006-05-09 | The Regents Of The University Of California | Chemical amplification based on fluid partitioning |
| DE10339452A1 (de) | 2003-08-22 | 2005-03-17 | Institut für Physikalische Hochtechnologie e.V. | Vorrichtung und Verfahren zur Strukturierung von Flüssigkeiten |
| WO2005021151A1 (en) | 2003-08-27 | 2005-03-10 | President And Fellows Of Harvard College | Electronic control of fluidic species |
| US20050069939A1 (en) | 2003-09-26 | 2005-03-31 | Youxiang Wang | Amplification of polynucleotides by rolling circle amplification |
| EP1691792A4 (en) | 2003-11-24 | 2008-05-28 | Yeda Res & Dev | COMPOSITIONS AND PROCESSES FOR SORTING IN VITRO OF MOLECULE AND CELL LIBRARIES |
| KR100647282B1 (ko) | 2003-12-24 | 2006-11-17 | 삼성전자주식회사 | DNA 마이크로어레이 스폿팅(Spoting) 장치 및이를 이용한 스폿팅 방법 |
| US7968287B2 (en) | 2004-10-08 | 2011-06-28 | Medical Research Council Harvard University | In vitro evolution in microfluidic systems |
| US20060201868A1 (en) | 2005-03-11 | 2006-09-14 | Simmons Blake A | Methods and devices for high-throughput dielectrophoretic concentration |
| WO2007024798A2 (en) | 2005-08-22 | 2007-03-01 | Applera Corporation | Apparatus, system, and method using immiscible-fluid-discrete-volumes |
| US8383059B2 (en) | 2005-09-30 | 2013-02-26 | University Of Utah Research Foundation | Microfluidic interface for highly parallel addressing of sensing arrays |
| US9183349B2 (en) | 2005-12-16 | 2015-11-10 | Nextbio | Sequence-centric scientific information management |
| WO2007081385A2 (en) | 2006-01-11 | 2007-07-19 | Raindance Technologies, Inc. | Microfluidic devices and methods of use in the formation and control of nanoreactors |
| US7998708B2 (en) | 2006-03-24 | 2011-08-16 | Handylab, Inc. | Microfluidic system for amplifying and detecting polynucleotides in parallel |
| EP2530167A1 (en) | 2006-05-11 | 2012-12-05 | Raindance Technologies, Inc. | Microfluidic Devices |
| CA2653321A1 (en) | 2006-05-26 | 2007-12-06 | Althea Technologies, Inc. | Biochemical analysis of partitioned cells |
| NZ573187A (en) | 2006-06-02 | 2011-11-25 | Pfizer Prod Inc | Circulating tumor cell assay for predicting efficacy of 1gf-1r antagonist therapy |
| ES2586210T3 (es) | 2006-12-14 | 2016-10-13 | Sangamo Biosciences, Inc. | Proteínas de dedo de zinc no canónicas optimizadas |
| US8338166B2 (en) | 2007-01-04 | 2012-12-25 | Lawrence Livermore National Security, Llc | Sorting, amplification, detection, and identification of nucleic acid subsequences in a complex mixture |
| US9029085B2 (en) | 2007-03-07 | 2015-05-12 | President And Fellows Of Harvard College | Assays and other reactions involving droplets |
| US8454906B2 (en) | 2007-07-24 | 2013-06-04 | The Regents Of The University Of California | Microfabricated droplet generator for single molecule/cell genetic analysis in engineered monodispersed emulsions |
| US8268564B2 (en) | 2007-09-26 | 2012-09-18 | President And Fellows Of Harvard College | Methods and applications for stitched DNA barcodes |
| GB0720202D0 (en) * | 2007-10-16 | 2007-11-28 | Cambridge Entpr Ltd | Microfluidic systems |
| WO2009092106A1 (en) | 2008-01-18 | 2009-07-23 | The Regents Of The University Of California | Accurate and rapid micromixer for integrated microfluidic devices |
| WO2009111014A2 (en) | 2008-03-04 | 2009-09-11 | Crystal Bioscience Inc. | Gel microdrop composition and method of using the same |
| US9409177B2 (en) | 2008-03-21 | 2016-08-09 | Lawrence Livermore National Security, Llc | Chip-based device for parallel sorting, amplification, detection, and identification of nucleic acid subsequences |
| WO2009137415A2 (en) | 2008-05-03 | 2009-11-12 | Advanced Liquid Logic, Inc. | Reagent and sample preparation, loading, and storage |
| WO2013016459A1 (en) | 2011-07-25 | 2013-01-31 | Bio-Rad Laboratories, Inc. | Breakage of an emulsion containing nucleic acid |
| US20130084572A1 (en) | 2011-09-30 | 2013-04-04 | Quantalife, Inc. | Calibrations and controls for droplet-based assays |
| US20140179544A1 (en) | 2012-09-07 | 2014-06-26 | Bio-Rad Laboratories, Inc. | Compositions, systems and methods for droplet formation, spacing and detection |
| US8709762B2 (en) | 2010-03-02 | 2014-04-29 | Bio-Rad Laboratories, Inc. | System for hot-start amplification via a multiple emulsion |
| US9132394B2 (en) | 2008-09-23 | 2015-09-15 | Bio-Rad Laboratories, Inc. | System for detection of spaced droplets |
| WO2011120020A1 (en) | 2010-03-25 | 2011-09-29 | Quantalife, Inc. | Droplet transport system for detection |
| US9156010B2 (en) | 2008-09-23 | 2015-10-13 | Bio-Rad Laboratories, Inc. | Droplet-based assay system |
| JP5457222B2 (ja) | 2009-02-25 | 2014-04-02 | エフ.ホフマン−ラ ロシュ アーゲー | 小型化ハイスループット核酸分析 |
| EP2977455B1 (en) | 2009-06-15 | 2020-04-15 | Complete Genomics, Inc. | Method for long fragment read sequencing |
| CN102483424B (zh) | 2009-06-26 | 2014-11-05 | 哈佛学院院长等 | 用于流体操作的方法以及微流体装置 |
| CA2767056C (en) | 2009-09-02 | 2018-12-04 | Bio-Rad Laboratories, Inc. | System for mixing fluids by coalescence of multiple emulsions |
| US8746285B2 (en) | 2009-09-04 | 2014-06-10 | Auburn University | Programmable fluidic droplet generation |
| US9376713B2 (en) | 2009-09-23 | 2016-06-28 | The Board Of Trustees Of The University Of Illinois | Label free detection of nucleic acid amplification |
| EP2488656B1 (en) | 2009-10-15 | 2015-06-03 | Ibis Biosciences, Inc. | Multiple displacement amplification |
| US8835358B2 (en) | 2009-12-15 | 2014-09-16 | Cellular Research, Inc. | Digital counting of individual molecules by stochastic attachment of diverse labels |
| US9315857B2 (en) | 2009-12-15 | 2016-04-19 | Cellular Research, Inc. | Digital counting of individual molecules by stochastic attachment of diverse label-tags |
| US20120270739A1 (en) | 2010-01-19 | 2012-10-25 | Verinata Health, Inc. | Method for sample analysis of aneuploidies in maternal samples |
| AU2013203624B2 (en) | 2010-02-09 | 2015-06-25 | General Electric Company | Isothermal amplification of nucleic acid using a mixture of randomized primers and specific primers |
| US9366632B2 (en) | 2010-02-12 | 2016-06-14 | Raindance Technologies, Inc. | Digital analyte analysis |
| CA2767182C (en) | 2010-03-25 | 2020-03-24 | Bio-Rad Laboratories, Inc. | Droplet generation for droplet-based assays |
| JP5573335B2 (ja) | 2010-04-28 | 2014-08-20 | 株式会社島津製作所 | 磁性体粒子操作デバイス及び磁性体粒子操作方法 |
| ES2539047T3 (es) | 2010-06-10 | 2015-06-25 | Albert-Ludwigs-Universität Freiburg | Aparato y método para dispensar células o partículas confinadas en una gota en vuelo libre |
| US9650629B2 (en) | 2010-07-07 | 2017-05-16 | Roche Molecular Systems, Inc. | Clonal pre-amplification in emulsion |
| US8465707B2 (en) | 2010-07-22 | 2013-06-18 | Gencell Biosystems Ltd. | Composite liquid cells |
| US9695390B2 (en) | 2010-08-23 | 2017-07-04 | President And Fellows Of Harvard College | Acoustic waves in microfluidics |
| US9562897B2 (en) | 2010-09-30 | 2017-02-07 | Raindance Technologies, Inc. | Sandwich assays in droplets |
| EP2436444A1 (en) | 2010-10-01 | 2012-04-04 | Centre National de la Recherche Scientifique (C.N.R.S.) | Microfluidic device for production and collection of droplets of a fluid |
| GB2497912B (en) | 2010-10-08 | 2014-06-04 | Harvard College | High-throughput single cell barcoding |
| US20120258870A1 (en) | 2010-11-22 | 2012-10-11 | The University Of Chicago | Methods, Systems, and/or Use of Oligonucleotide Conjugates to Develop Panels for Use in Assays and Detections |
| WO2012083225A2 (en) * | 2010-12-16 | 2012-06-21 | Gigagen, Inc. | System and methods for massively parallel analysis of nycleic acids in single cells |
| WO2012102663A1 (en) | 2011-01-24 | 2012-08-02 | Ge Healthcare Bio-Sciences Ab | Method of coupling binding agents to a substrate surface |
| US8765455B2 (en) | 2011-01-27 | 2014-07-01 | Lawrence Livermore National Security, Llc | Chip-based droplet sorting |
| WO2012106385A2 (en) * | 2011-01-31 | 2012-08-09 | Apprise Bio, Inc. | Methods of identifying multiple epitopes in cells |
| CN103443338B (zh) | 2011-02-02 | 2017-09-22 | 华盛顿大学商业化中心 | 大规模平行邻接作图 |
| ES2986436T3 (es) | 2011-04-15 | 2024-11-11 | Univ Johns Hopkins | Sistema de secuenciación segura |
| EP3395957B1 (en) | 2011-04-25 | 2020-08-12 | Bio-Rad Laboratories, Inc. | Methods and compositions for nucleic acid analysis |
| SG10201605049QA (en) | 2011-05-20 | 2016-07-28 | Fluidigm Corp | Nucleic acid encoding reactions |
| BR112013029729A2 (pt) | 2011-05-23 | 2017-01-24 | Basf Se | controle de emulsões, incluindo emulsões múltiplas |
| EP3216872B1 (en) * | 2011-06-02 | 2020-04-01 | Bio-Rad Laboratories, Inc. | Enzyme quantification |
| US8841071B2 (en) | 2011-06-02 | 2014-09-23 | Raindance Technologies, Inc. | Sample multiplexing |
| US8658430B2 (en) | 2011-07-20 | 2014-02-25 | Raindance Technologies, Inc. | Manipulating droplet size |
| AU2011373694A1 (en) | 2011-07-26 | 2013-05-02 | Verinata Health, Inc. | Method for determining the presence or absence of different aneuploidies in a sample |
| US20130032235A1 (en) | 2011-08-02 | 2013-02-07 | Teledyne Dalsa Semiconductor, Inc. | Integrated microfluidic check valve and device including such a check valve |
| US9469874B2 (en) * | 2011-10-18 | 2016-10-18 | The Regents Of The University Of California | Long-range barcode labeling-sequencing |
| WO2013095469A1 (en) | 2011-12-21 | 2013-06-27 | Intel Corporation | Psmi using at-speed scan capture |
| WO2013119753A1 (en) | 2012-02-08 | 2013-08-15 | President And Fellows Of Harvard College | Droplet formation using fluid breakup |
| EP3309262B1 (en) | 2012-02-24 | 2019-09-25 | Bio-Rad Laboratories, Inc. | Labeling and sample preparation for sequencing |
| EP2820174B1 (en) | 2012-02-27 | 2019-12-25 | The University of North Carolina at Chapel Hill | Methods and uses for molecular tags |
| EP3305918B1 (en) * | 2012-03-05 | 2020-06-03 | President and Fellows of Harvard College | Methods for epigenetic sequencing |
| PT2850211T (pt) * | 2012-05-14 | 2021-11-29 | Irepertoire Inc | Método para aumentar a precisão na deteção quantitativa de polinucleótidos |
| AU2013302867A1 (en) | 2012-08-13 | 2015-02-26 | The Regents Of The University Of California | Methods and systems for detecting biological components |
| US20140378345A1 (en) * | 2012-08-14 | 2014-12-25 | 10X Technologies, Inc. | Compositions and methods for sample processing |
| AU2013302756C1 (en) | 2012-08-14 | 2018-05-17 | 10X Genomics, Inc. | Microcapsule compositions and methods |
| WO2014047556A1 (en) | 2012-09-21 | 2014-03-27 | The Broad Institute, Inc. | Compositions and methods for long insert, paired end libraries of nucleic acids in emulsion droplets |
| CA2888042A1 (en) | 2012-11-27 | 2014-06-05 | Gencell Biosystems Ltd. | Handling liquid samples |
| CA2894694C (en) | 2012-12-14 | 2023-04-25 | 10X Genomics, Inc. | Methods and systems for processing polynucleotides |
| WO2014108323A1 (en) | 2013-01-10 | 2014-07-17 | Eth Zurich | Method and apparatus for depositing droplets onto a substrate |
| WO2014138132A2 (en) | 2013-03-04 | 2014-09-12 | Verax Biomedical Incorporated | Multi-analyte assay |
| WO2014153071A1 (en) | 2013-03-14 | 2014-09-25 | The Broad Institute, Inc. | Methods for quantitating dna using digital multiple displacement amplification |
| US9752179B2 (en) * | 2013-03-14 | 2017-09-05 | Cold Spring Harbor Laboratory | Trans-splicing transcriptome profiling |
| AU2014233699B2 (en) | 2013-03-15 | 2018-05-24 | The Regents Of The University Of California | High-speed on demand microfluidic droplet generation and manipulation |
| GB2525568B (en) * | 2013-03-15 | 2020-10-14 | Abvitro Llc | Single cell barcoding for antibody discovery |
| DE102013207900A1 (de) | 2013-04-30 | 2014-10-30 | Volkswagen Ag | Membran-Elektroden-Einheit und Brennstoffzelle mit einer solchen |
| KR102758333B1 (ko) | 2013-08-28 | 2025-01-23 | 벡톤 디킨슨 앤드 컴퍼니 | 대량의 동시 단일 세포 분석 |
| US10947587B2 (en) | 2013-11-05 | 2021-03-16 | The Regents Of The University Of California | Single-cell forensic short tandem repeat typing within microfluidic droplets |
| US10384187B2 (en) | 2014-02-10 | 2019-08-20 | Gencell Biosystems Ltd | Composite liquid cell (CLC) mediated nucleic acid library preparation device, and methods for using the same |
| CN113215221A (zh) | 2014-04-08 | 2021-08-06 | 华盛顿大学商业中心 | 用于使用多分散小滴执行数字检定的方法和设备 |
| AU2015250034B2 (en) | 2014-04-21 | 2019-06-20 | President And Fellows Of Harvard College | Systems and methods for barcoding nucleic acids |
| US20150298091A1 (en) | 2014-04-21 | 2015-10-22 | President And Fellows Of Harvard College | Systems and methods for barcoding nucleic acids |
| CA2949537C (en) | 2014-05-23 | 2023-02-14 | Firefly Bioworks, Inc. | Substrate-mediated reactors for bioassays |
| AU2015273480A1 (en) | 2014-06-11 | 2016-12-08 | Samplix S.A.R.L. | Nucleotide sequence exclusion enrichment by droplet sorting (needls) |
| WO2015200893A2 (en) * | 2014-06-26 | 2015-12-30 | 10X Genomics, Inc. | Methods of analyzing nucleic acids from individual cells or cell populations |
| US10697007B2 (en) | 2014-06-27 | 2020-06-30 | The Regents Of The University Of California | PCR-activated sorting (PAS) |
| WO2016064755A2 (en) | 2014-10-20 | 2016-04-28 | The Regents Of The University Of Califronia | Rapid modulation of droplet composition with membrane microvalves |
| US10434507B2 (en) | 2014-10-22 | 2019-10-08 | The Regents Of The University Of California | High definition microdroplet printer |
| CA2974299A1 (en) | 2015-02-04 | 2016-08-11 | The Regents Of The University Of California | Multiple-emulsion nucleic acid amplification |
| EP3253479B1 (en) | 2015-02-04 | 2022-09-21 | The Regents of The University of California | Sequencing of nucleic acids via barcoding in discrete entities |
| JP2018525004A (ja) | 2015-08-17 | 2018-09-06 | ザ リージェンツ オブ ザ ユニバーシティ オブ カリフォルニア | 微小液滴ベースの多置換増幅(mda)方法及び関連組成物 |
| US20190127789A1 (en) | 2016-04-19 | 2019-05-02 | President And Fellows Of Harvard College | Immobilization-based systems and methods for genetic analysis and other applications |
| EP3497228A4 (en) | 2016-08-10 | 2020-05-27 | The Regents of The University of California | MULTI-DISPLACEMENT AMPLIFICATION AND PCR COMBINED IN AN EMULSION MICRO DROPLET |
| CA3047328A1 (en) | 2016-12-21 | 2018-06-28 | The Regents Of The University Of California | Single cell genomic sequencing using hydrogel based droplets |
| EP4212629B1 (en) | 2017-11-17 | 2025-12-31 | 10X Genomics, Inc. | METHODS AND SYSTEMS FOR ASSOCIATING THE PHYSICAL AND GENETIC PROPERTIES OF BIOLOGICAL PARTICLES |
-
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- 2016-02-03 EP EP16747229.9A patent/EP3253479B1/en active Active
- 2016-02-03 US US15/015,015 patent/US11111519B2/en active Active
- 2016-02-03 AU AU2016215304A patent/AU2016215304B2/en active Active
- 2016-02-03 CN CN202210828875.5A patent/CN115011670A/zh active Pending
- 2016-02-03 CA CA2974306A patent/CA2974306A1/en active Pending
- 2016-02-03 CN CN201680020884.4A patent/CN107530654A/zh active Pending
- 2016-02-03 EP EP22190412.1A patent/EP4112744B1/en active Active
- 2016-02-03 WO PCT/US2016/016444 patent/WO2016126871A2/en not_active Ceased
- 2016-02-03 JP JP2017539228A patent/JP2018508198A/ja active Pending
-
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- 2018-03-29 US US15/940,850 patent/US11732287B2/en active Active
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- 2022-04-27 AU AU2022202780A patent/AU2022202780A1/en not_active Abandoned
-
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- 2023-06-22 US US18/212,868 patent/US12448643B2/en active Active
Patent Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101432439A (zh) * | 2006-02-24 | 2009-05-13 | 考利达基因组股份有限公司 | Dna阵列上的高通量基因组测序 |
| US20110059556A1 (en) * | 2009-09-04 | 2011-03-10 | The Research Foundation Of State University Of New York | Rapid and Continuous Analyte Processing in Droplet Microfluidic Devices |
| WO2012109600A2 (en) * | 2011-02-11 | 2012-08-16 | Raindance Technologies, Inc. | Methods for forming mixed droplets |
| US20120220494A1 (en) * | 2011-02-18 | 2012-08-30 | Raindance Technolgies, Inc. | Compositions and methods for molecular labeling |
| US20140378349A1 (en) * | 2012-08-14 | 2014-12-25 | 10X Technologies, Inc. | Compositions and methods for sample processing |
| US20140323316A1 (en) * | 2013-03-15 | 2014-10-30 | Complete Genomics, Inc. | Multiple tagging of individual long dna fragments |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN120783873A (zh) * | 2025-07-09 | 2025-10-14 | 上海锦测医学检验所有限公司 | 一种智能化蛋白质组学数据异常检测与分析方法 |
| CN120783873B (zh) * | 2025-07-09 | 2025-12-05 | 上海锦测医学检验所有限公司 | 一种智能化蛋白质组学数据异常检测与分析方法 |
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