CN114929732A - 一种重组人神经生长因子的制备方法 - Google Patents

一种重组人神经生长因子的制备方法 Download PDF

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Publication number
CN114929732A
CN114929732A CN202080086412.5A CN202080086412A CN114929732A CN 114929732 A CN114929732 A CN 114929732A CN 202080086412 A CN202080086412 A CN 202080086412A CN 114929732 A CN114929732 A CN 114929732A
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solution
inclusion body
washing
urea
inclusion
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朱祯平
黄浩旻
肖建国
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Sunshine Guojian Pharmaceutical Shanghai Co Ltd
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Sunshine Guojian Pharmaceutical Shanghai Co Ltd
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/70—Vectors or expression systems specially adapted for E. coli
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/14—Extraction; Separation; Purification
    • C07K1/16—Extraction; Separation; Purification by chromatography
    • C07K1/20—Partition-, reverse-phase or hydrophobic interaction chromatography
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/14—Extraction; Separation; Purification
    • C07K1/30—Extraction; Separation; Purification by precipitation
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/14—Extraction; Separation; Purification
    • C07K1/34—Extraction; Separation; Purification by filtration, ultrafiltration or reverse osmosis
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/14—Extraction; Separation; Purification
    • C07K1/36—Extraction; Separation; Purification by a combination of two or more processes of different types
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/475—Growth factors; Growth regulators
    • C07K14/48—Nerve growth factor [NGF]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P21/00—Preparation of peptides or proteins
    • C12P21/02—Preparation of peptides or proteins having a known sequence of two or more amino acids, e.g. glutathione
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12R—INDEXING SCHEME ASSOCIATED WITH SUBCLASSES C12C - C12Q, RELATING TO MICROORGANISMS
    • C12R2001/00—Microorganisms ; Processes using microorganisms
    • C12R2001/01—Bacteria or Actinomycetales ; using bacteria or Actinomycetales
    • C12R2001/185—Escherichia
    • C12R2001/19—Escherichia coli

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  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Biochemistry (AREA)
  • Molecular Biology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biophysics (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • General Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • Medicinal Chemistry (AREA)
  • Biomedical Technology (AREA)
  • Microbiology (AREA)
  • Analytical Chemistry (AREA)
  • Plant Pathology (AREA)
  • Physics & Mathematics (AREA)
  • Toxicology (AREA)
  • Gastroenterology & Hepatology (AREA)
  • General Chemical & Material Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Water Supply & Treatment (AREA)
  • Peptides Or Proteins (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)

Abstract

提供了一种重组人神经生长因子的制备方法，可以作为治疗药物。

Description

PCT国内申请，说明书已公开。

Claims (11)

  1. PCT国内申请，权利要求书已公开。
CN202080086412.5A 2019-12-20 2020-11-03 一种重组人神经生长因子的制备方法 Pending CN114929732A (zh)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
CN2019113253400 2019-12-20
CN201911325340.0A CN113004388A (zh) 2019-12-20 2019-12-20 一种重组人神经生长因子的制备方法
PCT/CN2020/126147 WO2021120900A1 (zh) 2019-12-20 2020-11-03 一种重组人神经生长因子的制备方法

Publications (1)

Publication Number Publication Date
CN114929732A true CN114929732A (zh) 2022-08-19

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CN201911325340.0A Pending CN113004388A (zh) 2019-12-20 2019-12-20 一种重组人神经生长因子的制备方法
CN202080086412.5A Pending CN114929732A (zh) 2019-12-20 2020-11-03 一种重组人神经生长因子的制备方法

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CN201911325340.0A Pending CN113004388A (zh) 2019-12-20 2019-12-20 一种重组人神经生长因子的制备方法

Country Status (6)

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US (1) US20230037311A1 (zh)
EP (1) EP4079754A4 (zh)
JP (1) JP2023507298A (zh)
CN (2) CN113004388A (zh)
TW (1) TWI773019B (zh)
WO (1) WO2021120900A1 (zh)

Families Citing this family (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2023004784A1 (zh) 2021-07-30 2023-02-02 成都优洛生物科技有限公司 一种重组cho细胞、其构建方法、利用其的检测系统和方法
CN115894705A (zh) * 2021-09-30 2023-04-04 三生国健药业（上海）股份有限公司 一种β-NGF融合蛋白、其制备方法及用途

Family Cites Families (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5986070A (en) 1990-04-06 1999-11-16 Amgen Inc. Production of biologically active NGF proteins
CN1219795C (zh) * 2000-07-19 2005-09-21 深圳市海王英特龙生物技术股份有限公司 用新的复性方法制备重组人神经生长因子
CN102628058B (zh) * 2012-03-23 2013-06-26 杭州纽龙生物科技有限公司 制备重组人源神经生长因子蛋白质的方法及复性液
CN102839182B (zh) * 2012-08-07 2013-10-16 武汉海特生物制药股份有限公司 利用大肠杆菌表达系统制备重组人神经生长因子的方法
EP2889304A1 (en) * 2013-12-27 2015-07-01 Sandoz Ag Method for refolding recombinantly produced polypeptides
CN103880943A (zh) * 2014-01-20 2014-06-25 厦门北大之路生物工程有限公司 一种rhNGF成熟肽的制备方法
CN103880944B (zh) * 2014-01-20 2017-03-29 未名生物医药有限公司 一种野生型rhNGF的制备方法
CN107973848B (zh) * 2017-12-28 2020-10-16 未名生物医药有限公司 一种从混合物中分离天然序列神经生长因子的方法

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Publication number Publication date
EP4079754A4 (en) 2024-06-19
TWI773019B (zh) 2022-08-01
WO2021120900A1 (zh) 2021-06-24
CN113004388A (zh) 2021-06-22
TW202128738A (zh) 2021-08-01
JP2023507298A (ja) 2023-02-22
EP4079754A1 (en) 2022-10-26
US20230037311A1 (en) 2023-02-09

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