CN114848680B - Composition for treating cow mastitis and preparation method thereof - Google Patents
Composition for treating cow mastitis and preparation method thereof Download PDFInfo
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Abstract
The invention belongs to the technical field of veterinary medicines, and provides a composition for treating cow mastitis and a preparation method thereof. The raw materials of the composition for treating cow mastitis comprise periplaneta americana extract. The composition has few raw material medicines, short time for treating cow mastitis, obvious effect, no toxic or side effect and difficult generation of drug resistance. The preparation method comprises the following steps: (1) preparation of periplaneta americana extract; (2) preparation of the composition. The preparation method is simple and efficient, and is suitable for large-scale industrial production.
Description
Technical Field
The invention belongs to the technical field of veterinary medicines, and particularly relates to a composition for treating dairy cow mastitis and a preparation method thereof.
Background
Cow mastitis, also known as cow mastitis, is one of the most common diseases of cows, and the cause of the cow mastitis is mainly caused by mechanical stimulation, pathogenic microorganism immersion and chemical physical injury. Cow mastitis not only affects milk yield, causing economic loss, but also affects milk quality, and endangers human health. In summer, the dairy cows are more prone to mastitis due to the influence of factors such as hot weather, dampness, poor sanitary conditions and the like.
Cow mastitis is classified into serous mastitis, cellulose catarrh mastitis, suppurative mastitis, hemorrhagic mastitis, gangrene mastitis and recessive mastitis. The main symptom set appears in the following three aspects: (1) the breast has inflammation manifestations such as red, swelling, heat, pain and the like; (2) reduced or stopped lactation; (3) the milk changes.
Cow mastitis is mainly caused by staphylococcus aureus, streptococcus and escherichia coli, and is usually treated by antibiotics such as penicillin, gentamicin, lincomycin and the like.
Antibiotics, although fast in effect, are easy to produce drug resistance, high in recurrence rate and easy to remain chemical drugs in milk. In recent years, research and clinical practice prove that the Chinese herbal medicine has great potential in treating cow mastitis, has no toxic or side effect, and is not easy to generate drug resistance by pathogenic bacteria.
The invention patent No. CN102488814B provides a traditional Chinese medicine composition for treating cow mastitis, and specifically discloses a traditional Chinese medicine composition for treating cow mastitis, which comprises, by weight, 15-30 parts of raw material medicines of red sage root, 5-10 parts of Chinese angelica, 1-5 parts of astragalus, 5-10 parts of red paeony root, 5-10 parts of rhizoma corydalis, 5-10 parts of nutgrass galingale rhizome, 2-5 parts of branch shell, 5-10 parts of green tangerine peel, 15-20 parts of honeysuckle, 5-10 parts of dandelion and 3-5 parts of liquorice. The composition can be prepared into paste, various raw materials are crushed to obtain fine powder of various raw materials, the crushed fine powder of various raw materials is stirred and mixed uniformly to obtain mixed powder, then vinegar and medical vaseline are added into the mixed powder, and the mixed powder is stirred uniformly to form paste. In the Chinese patent medicine formula, the medicines are combined, and the Chinese patent medicine has the functions of soothing liver, removing blood stasis, regulating blood, stopping bleeding, promoting qi circulation, relieving pain, resisting bacteria, diminishing inflammation, tonifying qi, strengthening exterior, promoting tissue regeneration, enhancing organism immunity and the like. The Chinese patent medicine has remarkable effect of treating and preventing cow mastitis, and has low cost and no toxic or side effect. The traditional Chinese medicine composition has certain effect of treating and preventing cow mastitis, but the traditional Chinese medicine composition still has the defects of more adopted raw medicines, poor efficacy and the like.
Disclosure of Invention
In view of the above-mentioned shortcomings in the prior art, a first object of the present invention is to provide a composition for treating cow mastitis, which has few raw material medicines, short time for treating cow mastitis, obvious effect, no toxic or side effect and difficult generation of drug resistance.
In view of the above-mentioned shortcomings in the prior art, a second object of the present invention is to provide a preparation method of a composition for treating cow mastitis, which is simple and efficient, and is suitable for large-scale industrial production.
In order to achieve the above object, the present invention adopts the following solutions:
a composition for treating cow mastitis comprises periplaneta americana extract as raw material.
A preparation method of the composition for treating cow mastitis comprises the following steps: (1) preparation of periplaneta americana extract: adding 1.5-2 times of water into Periplaneta americana coarse powder, flash extracting under 92-108V for 2-3 times and 2min each time to obtain extractive solution, and centrifuging to obtain Periplaneta americana extract
The composition for treating cow mastitis and the preparation method thereof provided by the invention have the beneficial effects that:
(1) The raw materials of the composition for treating cow mastitis provided by the invention comprise periplaneta americana extract. The main components of the American cockroach extract are peptides (containing EGF), alcohols, mucin and other 18 kinds of amino acids, reviving nucleosides, cardiovascular active peptides, high proteins and newly discovered various growth promoting factor groups; the periplaneta americana extract can obviously promote the proliferation of wound surfaces and granulation tissues of burn models, and has corresponding dose-effect relationship, and in addition, the periplaneta americana extract also has the effects of resisting virus, inflammation, oxidation, free radicals and pain. The inventor creatively discovers that the composition taking the periplaneta americana extract as the main raw material can greatly shorten the treatment duration and has obvious treatment effect when being used for treating the cow mammitis; the periplaneta americana extract is safe and has no toxic or side effect because of natural components.
(2) The preparation method of the composition for treating cow mastitis provided by the invention has the advantages that the content of active ingredients in the periplaneta americana extract obtained by the preparation method is high, and the preparation method is simple and efficient and is suitable for large-scale industrial production.
Detailed Description
In order to make the objects, technical solutions and advantages of the embodiments of the present invention more clear, the technical solutions of the embodiments of the present invention will be clearly and completely described below. The specific conditions are not noted in the examples and are carried out according to conventional conditions or conditions recommended by the manufacturer. The reagents or apparatus used were conventional products commercially available without the manufacturer's attention.
The following describes a composition for treating cow mastitis and a preparation method thereof.
The embodiment of the invention provides a composition for treating cow mastitis, which comprises the following raw materials of periplaneta americana extract and pearl dew grass extract. Wherein, the American cockroach extract is 10-20 parts by weight and the pearl dew grass extract is 8-13 parts by weight; further, the periplaneta americana extract is 12-15 parts, and the pearl dew grass extract is 9-11 parts; further, the periplaneta americana extract is 13 parts, and the pearl dew grass extract is 10 parts.
The pearl grass, also called the sad grass or the blue grass, is the root of the plant spider silk blue grass, belonging to Commelinaceae; the pearl water grass has the effects of dredging collaterals, relieving pain, promoting diuresis and relieving swelling; is used for treating rheumatalgia, lumbago, skelalgia, numbness of limbs, edema and eczema; the main component of the pearl dew grass extract is beta-ecdysone, and the beta-ecdysone is one of polyhydroxy hormones, can promote cell growth, stimulate dermal cell division, and play a role in ecdysis promotion.
The inventor creatively discovers that when the periplaneta americana extract and the pearl dew grass extract are used in combination, the periplaneta americana extract and the pearl dew grass extract can generate obvious synergistic effect, the treatment duration can be greatly shortened in the treatment of the dairy cow mammitis, and the treatment effect is obvious; the periplaneta americana extract and the pearl dew grass extract are natural in components, so that the periplaneta americana extract and the pearl dew grass extract are safe and have no toxic or side effects.
In the proportion range, the periplaneta americana extract and the pearl dew grass extract have excellent synergistic effect, can greatly shorten the treatment duration, and have obvious treatment effect.
The embodiment of the invention also provides a preparation method of the composition for treating the dairy cow mastitis, which comprises the following steps: (1) preparation of periplaneta americana extract: adding 1.5-2 times of water into the periplaneta americana coarse powder, performing flash extraction for 2-3 times under the condition of 92-108V for 2min each time to obtain an extract, and performing centrifugal separation on the extract at 3500-3800r/min for 30min to obtain the periplaneta americana extract. In this step, the parameters are cooperatively matched so that the obtained periplaneta americana extract is rich in active substances.
(2) Preparation of the Pearl dew grass extract: adding deionized water with the weight 7-9 times of that of the pearl dew grass into the pearl dew grass, boiling with strong fire, turning to low fire, continuously boiling for 30-40min, and filtering to obtain first filtrate and filter residue; adding deionized water with the weight 5-7 times of that of the filter residue, boiling with strong fire, and continuing boiling with small fire for 22-28min; filtering to obtain a second filtrate; combining the first filtrate and the second filtrate to obtain a pearl dew grass extract; in the step, in the two-time boiling and filtering process, the proportion of the pearl water grass to the deionized water can be matched in a synergistic way, so that the content of active ingredients in the filtrate can be improved.
(3) Preparation of the composition: mixing the pearl dew grass extract and the American cockroach extract according to parts by weight to obtain a mixed solution, adding 8-12% of chitosan, 101 juice clarifier or gelatin by weight into the mixed solution, stirring and mixing for 5-8min at the rotating speed of 600-800r/min, standing to remove grease and sediment, filtering and concentrating to obtain the composition. In the step, the mixture ratio of the natural clarifying agent and the mixed liquid and other technological parameters are adopted to cooperatively cooperate, so that grease and impurities in the mixed liquid can be removed, and the purity of the mixed liquid is improved. In this example, the dosage forms of the composition include ointments, sprays, film-coating agents and transdermal patches.
According to the preparation method, the American cockroach extract and the pearl dew grass extract are respectively obtained, and then are mixed and clarified, so that the effective components in the two extracts can be extracted to the greatest extent, and the purity of a final product can be improved. The preparation method is simple and efficient, and is suitable for large-scale industrial production.
The features and capabilities of the present invention are described in further detail below in connection with the examples.
Example 1
The embodiment provides a preparation method of a composition for treating cow mastitis, which comprises the following steps: (1) preparation of periplaneta americana extract: adding 1.8 times of water into the periplaneta americana coarse powder, performing flash extraction for 2 times under the condition of 100V voltage for 2min each time to obtain an extracting solution, and performing centrifugal separation on the extracting solution at 3600r/min for 30min to obtain periplaneta americana extract; (2) preparation of the composition: adding chitosan 10% of the weight of the periplaneta americana extract, stirring and mixing at the rotation speed of 700r/min for 6min, standing to remove grease and precipitate, filtering, and concentrating to two thirds of the volume of the periplaneta americana extract to obtain the spray composition.
Example 2
The embodiment provides a preparation method of a composition for treating cow mastitis, which comprises the following steps:
(1) Preparing the American cockroach extract: adding 1.8 times of water into the periplaneta americana coarse powder, performing flash extraction for 2 times under the condition of 100V voltage for 2min each time to obtain an extracting solution, and performing centrifugal separation on the extracting solution at 3600r/min for 30min to obtain periplaneta americana extract;
(2) Preparation of the Pearl dew grass extract: adding deionized water with the weight 8 times of that of the pearl dew grass into the pearl dew grass, boiling with big fire, turning into small fire, continuously boiling for 35min, and filtering to obtain first filtrate and filter residue; adding deionized water with the weight 6 times of that of the filter residue, boiling with strong fire, and continuing boiling with small fire for 25min; filtering to obtain a second filtrate; combining the first filtrate and the second filtrate to obtain a pearl dew grass extract;
(3) Preparation of the composition: according to parts by weight, 8 parts of pearl water weed extract and 20 parts of American cockroach extract are mixed to obtain mixed solution, 10 percent of chitosan with the weight of the mixed solution is added into the mixed solution, and the mixed solution is stirred and mixed for 6 minutes at the rotating speed of 700r/min, then the mixed solution is stood for removing grease and sediment, and is concentrated to two thirds of the volume of the mixed solution after being filtered to obtain the spray composition.
Example 3
The embodiment provides a preparation method of a composition for treating cow mastitis, which comprises the following steps:
(1) Preparing the American cockroach extract: adding 1.8 times of water into the periplaneta americana coarse powder, performing flash extraction for 2 times under the condition of 100V voltage for 2min each time to obtain an extracting solution, and performing centrifugal separation on the extracting solution at 3600r/min for 30min to obtain periplaneta americana extract;
(2) Preparation of the Pearl dew grass extract: adding deionized water with the weight 8 times of that of the pearl dew grass into the pearl dew grass, boiling with big fire, turning into small fire, continuously boiling for 35min, and filtering to obtain first filtrate and filter residue; adding deionized water with the weight 6 times of that of the filter residue, boiling with strong fire, and continuing boiling with small fire for 25min; filtering to obtain a second filtrate; combining the first filtrate and the second filtrate to obtain a pearl dew grass extract;
(3) Preparation of the composition: 13 parts of pearl water weed extract and 10 parts of American cockroach extract are mixed according to parts by weight to obtain mixed solution, 101 juice clarifying agent with 10 percent of the weight of the mixed solution is added into the mixed solution, and the mixed solution is stirred and mixed for 6 minutes at the rotating speed of 700r/min, then the mixed solution is stood for removing grease and sediment, and is concentrated to two thirds of the volume of the mixed solution after being filtered to obtain the spray composition.
Example 4
The embodiment provides a preparation method of a composition for treating cow mastitis, which comprises the following steps:
(1) Preparing the American cockroach extract: adding 1.8 times of water into the periplaneta americana coarse powder, performing flash extraction for 2 times under the condition of 100V voltage for 2min each time to obtain an extracting solution, and performing centrifugal separation on the extracting solution at 3600r/min for 30min to obtain periplaneta americana extract;
(2) Preparation of the Pearl dew grass extract: adding deionized water with the weight 8 times of that of the pearl dew grass into the pearl dew grass, boiling with big fire, turning into small fire, continuously boiling for 35min, and filtering to obtain first filtrate and filter residue; adding deionized water with the weight 6 times of that of the filter residue, boiling with strong fire, and continuing boiling with small fire for 25min; filtering to obtain a second filtrate; combining the first filtrate and the second filtrate to obtain a pearl dew grass extract;
(3) Preparation of the composition: according to parts by weight, 9 parts of pearl water weed extract and 15 parts of American cockroach extract are mixed to obtain mixed solution, 10% of gelatin of the weight of the mixed solution is added into the mixed solution, the mixed solution is stirred and mixed for 6min at the rotating speed of 700r/min, and then the mixed solution is stood for removing grease and sediment, and is concentrated to two thirds of the volume of the mixed solution after being filtered to obtain the spray composition.
Example 5
The embodiment provides a preparation method of a composition for treating cow mastitis, which comprises the following steps:
(1) Preparing the American cockroach extract: adding 1.8 times of water into the periplaneta americana coarse powder, performing flash extraction for 2 times under the condition of 100V voltage for 2min each time to obtain an extracting solution, and performing centrifugal separation on the extracting solution at 3600r/min for 30min to obtain periplaneta americana extract;
(2) Preparation of the Pearl dew grass extract: adding deionized water with the weight 8 times of that of the pearl dew grass into the pearl dew grass, boiling with big fire, turning into small fire, continuously boiling for 35min, and filtering to obtain first filtrate and filter residue; adding deionized water with the weight 6 times of that of the filter residue, boiling with strong fire, and continuing boiling with small fire for 25min; filtering to obtain a second filtrate; combining the first filtrate and the second filtrate to obtain a pearl dew grass extract;
(3) Preparation of the composition: according to parts by weight, 11 parts of pearl water weed extract and 12 parts of American cockroach extract are mixed to obtain mixed solution, 10% of gelatin of the weight of the mixed solution is added into the mixed solution, the mixed solution is stirred and mixed for 6min at the rotating speed of 700r/min, and then the mixed solution is stood for removing grease and sediment, and is filtered and concentrated to two thirds of the volume of the mixed solution to prepare the spray composition.
Example 6
The embodiment provides a preparation method of a composition for treating cow mastitis, which comprises the following steps:
(1) Preparing the American cockroach extract: adding 1.8 times of water into the periplaneta americana coarse powder, performing flash extraction for 2 times under the condition of 100V voltage for 2min each time to obtain an extracting solution, and performing centrifugal separation on the extracting solution at 3600r/min for 30min to obtain periplaneta americana extract;
(2) Preparation of the Pearl dew grass extract: adding deionized water with the weight 8 times of that of the pearl dew grass into the pearl dew grass, boiling with big fire, turning into small fire, continuously boiling for 35min, and filtering to obtain first filtrate and filter residue; adding deionized water with the weight 6 times of that of the filter residue, boiling with strong fire, and continuing boiling with small fire for 25min; filtering to obtain a second filtrate; combining the first filtrate and the second filtrate to obtain a pearl dew grass extract;
(3) Preparation of the composition: 13 parts of pearl water weed extract and 10 parts of American cockroach extract are mixed according to parts by weight to obtain mixed solution, 10 percent of gelatin of the weight of the mixed solution is added into the mixed solution, and the mixed solution is stirred and mixed for 6 minutes at the rotating speed of 700r/min, then the mixed solution is stood for removing grease and sediment, and is filtered and concentrated to two thirds of the volume of the mixed solution to prepare the spray composition.
Example 7
The embodiment provides a preparation method of a composition for treating cow mastitis, which comprises the following steps:
(1) Preparing the American cockroach extract: adding 1.8 times of water into the periplaneta americana coarse powder, performing flash extraction for 2 times under the condition of 100V voltage for 2min each time to obtain an extracting solution, and performing centrifugal separation on the extracting solution at 3600r/min for 30min to obtain periplaneta americana extract;
(2) Preparation of the Pearl dew grass extract: adding deionized water with the weight 7 times of that of the pearl dew grass into the pearl dew grass, boiling with big fire, turning into small fire, continuously boiling for 30min, and filtering to obtain first filtrate and filter residue; adding deionized water with the weight 5 times of that of the filter residue, boiling with strong fire, and continuing boiling with small fire for 22min; filtering to obtain a second filtrate; combining the first filtrate and the second filtrate to obtain a pearl dew grass extract;
(3) Preparation of the composition: 13 parts of pearl water weed extract and 10 parts of American cockroach extract are mixed according to parts by weight to obtain mixed solution, 10 percent of gelatin of the weight of the mixed solution is added into the mixed solution, and the mixed solution is stirred and mixed for 6 minutes at the rotating speed of 700r/min, then the mixed solution is stood for removing grease and sediment, and is filtered and concentrated to two thirds of the volume of the mixed solution to prepare the spray composition.
Example 8
The embodiment provides a preparation method of a composition for treating cow mastitis, which comprises the following steps:
(1) Preparing the American cockroach extract: adding 1.8 times of water into the periplaneta americana coarse powder, performing flash extraction for 2 times under the condition of 100V voltage for 2min each time to obtain an extracting solution, and performing centrifugal separation on the extracting solution at 3600r/min for 30min to obtain periplaneta americana extract;
(2) Preparation of the Pearl dew grass extract: adding deionized water with the weight 9 times of that of the pearl dew grass into the pearl dew grass, boiling with big fire, turning into small fire, continuously boiling for 40min, and filtering to obtain first filtrate and filter residue; adding deionized water with the weight 7 times of that of the filter residue, boiling with strong fire, and continuing boiling with small fire for 28min; filtering to obtain a second filtrate; combining the first filtrate and the second filtrate to obtain a pearl dew grass extract;
(3) Preparation of the composition: 13 parts of pearl water weed extract and 10 parts of American cockroach extract are mixed according to parts by weight to obtain mixed solution, 10 percent of gelatin of the weight of the mixed solution is added into the mixed solution, and the mixed solution is stirred and mixed for 6 minutes at the rotating speed of 700r/min, then the mixed solution is stood for removing grease and sediment, and is filtered and concentrated to two thirds of the volume of the mixed solution to prepare the spray composition.
Example 9
The embodiment provides a preparation method of a composition for treating cow mastitis, which comprises the following steps:
(1) Preparing the American cockroach extract: adding 1.5 times of water into the periplaneta americana coarse powder, performing flash extraction for 3 times under the condition of 108V for 2min each time to obtain an extracting solution, and performing centrifugal separation on the extracting solution at 3500r/min for 30min to obtain periplaneta americana extract;
(2) Preparation of the Pearl dew grass extract: adding deionized water with the weight 8 times of that of the pearl dew grass into the pearl dew grass, boiling with big fire, turning into small fire, continuously boiling for 35min, and filtering to obtain first filtrate and filter residue; adding deionized water with the weight 6 times of that of the filter residue, boiling with strong fire, and continuing boiling with small fire for 25min; filtering to obtain a second filtrate; combining the first filtrate and the second filtrate to obtain a pearl dew grass extract;
(3) Preparation of the composition: 13 parts of pearl water weed extract and 10 parts of American cockroach extract are mixed according to parts by weight to obtain mixed solution, 10 percent of gelatin of the weight of the mixed solution is added into the mixed solution, and the mixed solution is stirred and mixed for 6 minutes at the rotating speed of 700r/min, then the mixed solution is stood for removing grease and sediment, and is filtered and concentrated to two thirds of the volume of the mixed solution to prepare the spray composition.
Example 10
The embodiment provides a preparation method of a composition for treating cow mastitis, which comprises the following steps:
(1) Preparing the American cockroach extract: adding water with the weight 2 times of the periplaneta americana coarse powder into the periplaneta americana coarse powder, performing flash extraction for 2 times under the condition of 92V for 2min each time to obtain an extracting solution, and performing centrifugal separation on the extracting solution at 3800r/min for 30min to obtain periplaneta americana extract;
(2) Preparation of the Pearl dew grass extract: adding deionized water with the weight 8 times of that of the pearl dew grass into the pearl dew grass, boiling with big fire, turning into small fire, continuously boiling for 35min, and filtering to obtain first filtrate and filter residue; adding deionized water with the weight 6 times of that of the filter residue, boiling with strong fire, and continuing boiling with small fire for 25min; filtering to obtain a second filtrate; combining the first filtrate and the second filtrate to obtain a pearl dew grass extract;
(3) Preparation of the composition: 13 parts of pearl water weed extract and 10 parts of American cockroach extract are mixed according to parts by weight to obtain mixed solution, 10 percent of gelatin of the weight of the mixed solution is added into the mixed solution, and the mixed solution is stirred and mixed for 6 minutes at the rotating speed of 700r/min, then the mixed solution is stood for removing grease and sediment, and is filtered and concentrated to two thirds of the volume of the mixed solution to prepare the spray composition.
Example 11
The embodiment provides a preparation method of a composition for treating cow mastitis, which comprises the following steps:
(1) Preparing the American cockroach extract: adding 1.8 times of water into the periplaneta americana coarse powder, performing flash extraction for 2 times under the condition of 100V voltage for 2min each time to obtain an extracting solution, and performing centrifugal separation on the extracting solution at 3600r/min for 30min to obtain periplaneta americana extract;
(2) Preparation of the Pearl dew grass extract: adding deionized water with the weight 8 times of that of the pearl dew grass into the pearl dew grass, boiling with big fire, turning into small fire, continuously boiling for 35min, and filtering to obtain first filtrate and filter residue; adding deionized water with the weight 6 times of that of the filter residue, boiling with strong fire, and continuing boiling with small fire for 25min; filtering to obtain a second filtrate; combining the first filtrate and the second filtrate to obtain a pearl dew grass extract;
(3) Preparation of the composition: 13 parts of pearl water weed extract and 10 parts of American cockroach extract are mixed according to parts by weight to obtain mixed solution, 10 percent of gelatin of the weight of the mixed solution is added into the mixed solution, and the mixed solution is stirred and mixed for 8 minutes at the rotating speed of 600r/min, then the mixed solution is stood for removing grease and sediment, and is filtered and concentrated to two thirds of the volume of the mixed solution to prepare the spray composition.
Example 12
The embodiment provides a preparation method of a composition for treating cow mastitis, which comprises the following steps:
(1) Preparing the American cockroach extract: adding 1.8 times of water into the periplaneta americana coarse powder, performing flash extraction for 2 times under the condition of 100V voltage for 2min each time to obtain an extracting solution, and performing centrifugal separation on the extracting solution at 3600r/min for 30min to obtain periplaneta americana extract;
(2) Preparation of the Pearl dew grass extract: adding deionized water with the weight 8 times of that of the pearl dew grass into the pearl dew grass, boiling with big fire, turning into small fire, continuously boiling for 35min, and filtering to obtain first filtrate and filter residue; adding deionized water with the weight 6 times of that of the filter residue, boiling with strong fire, and continuing boiling with small fire for 25min; filtering to obtain a second filtrate; combining the first filtrate and the second filtrate to obtain a pearl dew grass extract;
(3) Preparation of the composition: 13 parts of pearl water weed extract and 10 parts of American cockroach extract are mixed according to parts by weight to obtain mixed solution, 10 percent of gelatin of the weight of the mixed solution is added into the mixed solution, and the mixed solution is stirred and mixed for 5 minutes at the rotating speed of 800r/min, then the mixed solution is stood for removing grease and sediment, and is filtered and concentrated to two thirds of the volume of the mixed solution to prepare the spray composition.
Comparative example 1
The present comparative example provides a method for preparing a composition for treating bovine mastitis, comprising:
(1) Preparing the American cockroach extract: adding 1.8 times of water into the periplaneta americana coarse powder, performing flash extraction for 2 times under the condition of 100V voltage for 2min each time to obtain an extracting solution, and performing centrifugal separation on the extracting solution at 3600r/min for 30min to obtain periplaneta americana extract;
(2) Preparation of the Pearl dew grass extract: adding deionized water with the weight 6 times of that of the pearl dew grass into the pearl dew grass, boiling with big fire, turning into small fire, continuously boiling for 28min, and filtering to obtain first filtrate and filter residue; adding deionized water with the weight 4 times of that of the filter residue, boiling with strong fire, and continuing boiling with small fire for 20min; filtering to obtain a second filtrate; combining the first filtrate and the second filtrate to obtain a pearl dew grass extract;
(3) Preparation of the composition: 13 parts of pearl water weed extract and 10 parts of American cockroach extract are mixed according to parts by weight to obtain mixed solution, 10 percent of gelatin of the weight of the mixed solution is added into the mixed solution, and the mixed solution is stirred and mixed for 6 minutes at the rotating speed of 700r/min, then the mixed solution is stood for removing grease and sediment, and is filtered and concentrated to two thirds of the volume of the mixed solution to prepare the spray composition.
Comparative example 2
The present comparative example provides a method for preparing a composition for treating bovine mastitis, comprising:
(1) Preparing the American cockroach extract: adding 1.8 times of water into the periplaneta americana coarse powder, performing flash extraction for 2 times under the condition of 100V voltage for 2min each time to obtain an extracting solution, and performing centrifugal separation on the extracting solution at 3600r/min for 30min to obtain periplaneta americana extract;
(2) Preparation of the Pearl dew grass extract: adding deionized water with the weight 10 times of that of the pearl dew grass into the pearl dew grass, boiling with big fire, turning into small fire, continuously boiling for 42min, and filtering to obtain first filtrate and filter residue; adding deionized water with the weight 8 times of that of the filter residue into the filter residue, boiling with strong fire, and then continuously boiling with small fire for 30min; filtering to obtain a second filtrate; combining the first filtrate and the second filtrate to obtain a pearl dew grass extract;
(3) Preparation of the composition: 13 parts of pearl water weed extract and 10 parts of American cockroach extract are mixed according to parts by weight to obtain mixed solution, 10 percent of gelatin of the weight of the mixed solution is added into the mixed solution, and the mixed solution is stirred and mixed for 6 minutes at the rotating speed of 700r/min, then the mixed solution is stood for removing grease and sediment, and is filtered and concentrated to two thirds of the volume of the mixed solution to prepare the spray composition.
Comparative example 3
The present comparative example provides a method for preparing a composition for treating bovine mastitis, comprising:
(1) Preparing the American cockroach extract: adding 1.2 times of water into the periplaneta americana coarse powder, performing flash extraction for 1 time under the condition of 110V for 3min each time to obtain an extracting solution, and performing centrifugal separation on the extracting solution at 3500r/min for 30min to obtain periplaneta americana extract;
(2) Preparation of the Pearl dew grass extract: adding deionized water with the weight 8 times of that of the pearl dew grass into the pearl dew grass, boiling with big fire, turning into small fire, continuously boiling for 35min, and filtering to obtain first filtrate and filter residue; adding deionized water with the weight 6 times of that of the filter residue, boiling with strong fire, and continuing boiling with small fire for 25min; filtering to obtain a second filtrate; combining the first filtrate and the second filtrate to obtain a pearl dew grass extract;
(3) Preparation of the composition: 13 parts of pearl water weed extract and 10 parts of American cockroach extract are mixed according to parts by weight to obtain mixed solution, 10 percent of gelatin of the weight of the mixed solution is added into the mixed solution, and the mixed solution is stirred and mixed for 6 minutes at the rotating speed of 700r/min, then the mixed solution is stood for removing grease and sediment, and is filtered and concentrated to two thirds of the volume of the mixed solution to prepare the spray composition.
Comparative example 4
The present comparative example provides a method for preparing a composition for treating bovine mastitis, comprising:
(1) Preparing the American cockroach extract: adding water 3 times of the periplaneta americana coarse powder into the periplaneta americana coarse powder, performing flash extraction for 4 times under the condition of 90V voltage for 1min each time to obtain an extracting solution, and performing centrifugal separation on the extracting solution at 4000r/min for 30min to obtain periplaneta americana extract;
(2) Preparation of the Pearl dew grass extract: adding deionized water with the weight 8 times of that of the pearl dew grass into the pearl dew grass, boiling with big fire, turning into small fire, continuously boiling for 35min, and filtering to obtain first filtrate and filter residue; adding deionized water with the weight 6 times of that of the filter residue, boiling with strong fire, and continuing boiling with small fire for 25min; filtering to obtain a second filtrate; combining the first filtrate and the second filtrate to obtain a pearl dew grass extract;
(3) Preparation of the composition: 13 parts of pearl water weed extract and 10 parts of American cockroach extract are mixed according to parts by weight to obtain mixed solution, 10 percent of gelatin of the weight of the mixed solution is added into the mixed solution, and the mixed solution is stirred and mixed for 6 minutes at the rotating speed of 700r/min, then the mixed solution is stood for removing grease and sediment, and is filtered and concentrated to two thirds of the volume of the mixed solution to prepare the spray composition.
Comparative example 5
The present comparative example provides a method for preparing a composition for treating bovine mastitis, comprising:
(1) Preparing the American cockroach extract: adding 1.8 times of water into the periplaneta americana coarse powder, performing flash extraction for 2 times under the condition of 100V voltage for 2min each time to obtain an extracting solution, and performing centrifugal separation on the extracting solution at 3600r/min for 30min to obtain periplaneta americana extract;
(2) Preparation of the Pearl dew grass extract: adding deionized water with the weight 8 times of that of the pearl dew grass into the pearl dew grass, boiling with big fire, turning into small fire, continuously boiling for 35min, and filtering to obtain first filtrate and filter residue; adding deionized water with the weight 6 times of that of the filter residue, boiling with strong fire, and continuing boiling with small fire for 25min; filtering to obtain a second filtrate; combining the first filtrate and the second filtrate to obtain a pearl dew grass extract;
(3) Preparation of the composition: according to parts by weight, 14 parts of pearl water weed extract and 9 parts of American cockroach extract are mixed to obtain mixed solution, 10% of gelatin of the weight of the mixed solution is added into the mixed solution, the mixed solution is stirred and mixed for 6min at the rotating speed of 700r/min, and then the mixed solution is stood for removing grease and sediment, and is filtered and concentrated to two thirds of the volume of the mixed solution to prepare the spray composition.
Comparative example 6
The present comparative example provides a method for preparing a composition for treating bovine mastitis, comprising:
(1) Preparing the American cockroach extract: adding 1.8 times of water into the periplaneta americana coarse powder, performing flash extraction for 2 times under the condition of 100V voltage for 2min each time to obtain an extracting solution, and performing centrifugal separation on the extracting solution at 3600r/min for 30min to obtain periplaneta americana extract;
(2) Preparation of the Pearl dew grass extract: adding deionized water with the weight 8 times of that of the pearl dew grass into the pearl dew grass, boiling with big fire, turning into small fire, continuously boiling for 35min, and filtering to obtain first filtrate and filter residue; adding deionized water with the weight 6 times of that of the filter residue, boiling with strong fire, and continuing boiling with small fire for 25min; filtering to obtain a second filtrate; combining the first filtrate and the second filtrate to obtain a pearl dew grass extract;
(3) Preparation of the composition: according to parts by weight, 7 parts of pearl water weed extract and 21 parts of American cockroach extract are mixed to obtain mixed solution, 10% of gelatin of the weight of the mixed solution is added into the mixed solution, the mixed solution is stirred and mixed for 6min at the rotating speed of 700r/min, and then the mixed solution is stood for removing grease and sediment, and is filtered and concentrated to two thirds of the volume of the mixed solution to prepare the spray composition.
Comparative example 7
The present comparative example provides a method for preparing a composition for treating bovine mastitis, comprising:
(1) Preparing the American cockroach extract: adding 1.8 times of water into the periplaneta americana coarse powder, performing flash extraction for 2 times under the condition of 100V voltage for 2min each time to obtain an extracting solution, and performing centrifugal separation on the extracting solution at 3600r/min for 30min to obtain periplaneta americana extract;
(2) Preparation of the Pearl dew grass extract: adding deionized water with the weight 8 times of that of the pearl dew grass into the pearl dew grass, boiling with big fire, turning into small fire, continuously boiling for 35min, and filtering to obtain first filtrate and filter residue; adding deionized water with the weight 6 times of that of the filter residue, boiling with strong fire, and continuing boiling with small fire for 25min; filtering to obtain a second filtrate; combining the first filtrate and the second filtrate to obtain a pearl dew grass extract;
(3) Preparation of the composition: 13 parts of pearl water weed extract and 10 parts of American cockroach extract are mixed according to parts by weight to obtain mixed solution, 7% of gelatin of the weight of the mixed solution is added into the mixed solution, the mixed solution is stirred and mixed for 4min at the rotating speed of 900r/min, and then the mixed solution is stood for removing grease and sediment, and is concentrated to two thirds of the volume of the mixed solution after being filtered to obtain the spray composition.
Comparative example 8
The present comparative example provides a method for preparing a composition for treating bovine mastitis, comprising:
(1) Preparing the American cockroach extract: adding 1.8 times of water into the periplaneta americana coarse powder, performing flash extraction for 2 times under the condition of 100V voltage for 2min each time to obtain an extracting solution, and performing centrifugal separation on the extracting solution at 3600r/min for 30min to obtain periplaneta americana extract;
(2) Preparation of the Pearl dew grass extract: adding deionized water with the weight 8 times of that of the pearl dew grass into the pearl dew grass, boiling with big fire, turning into small fire, continuously boiling for 35min, and filtering to obtain first filtrate and filter residue; adding deionized water with the weight 6 times of that of the filter residue, boiling with strong fire, and continuing boiling with small fire for 25min; filtering to obtain a second filtrate; combining the first filtrate and the second filtrate to obtain a pearl dew grass extract;
(3) Preparation of the composition: 13 parts of pearl water weed extract and 10 parts of American cockroach extract are mixed according to parts by weight to obtain mixed solution, 13 percent of gelatin of the weight of the mixed solution is added into the mixed solution, and the mixed solution is stirred and mixed for 9 minutes at the rotating speed of 500r/min, then the mixed solution is stood for removing grease and sediment, and is filtered and concentrated to two thirds of the volume of the mixed solution to prepare the spray composition.
Comparative example 9
The comparative example provides a preparation method of a pearl water weed extract paste, which comprises the following steps: preparation of the Pearl dew grass extract: adding deionized water with the weight 8 times of that of the pearl dew grass into the pearl dew grass, boiling with big fire, turning into small fire, continuously boiling for 35min, and filtering to obtain first filtrate and filter residue; adding deionized water with the weight 6 times of that of the filter residue, boiling with strong fire, and continuing boiling with small fire for 25min; filtering to obtain a second filtrate; combining the first filtrate and the second filtrate to obtain a pearl dew grass extract; adding gelatin 10% of its own weight into the herba Phyllanthi Urinariae extract, stirring at 700r/min for 6min, standing to remove oil and precipitate, filtering, and concentrating to two thirds of its volume to obtain herba Phyllanthi Urinariae extract spray.
Experimental example 1 in vitro bacteriostasis test for bovine mastitis
The experimental method comprises the following steps:
(1) Main reagent and culture medium: beef extract, peptone, yeast powder, agar powder and common agar medium;
(2) Test strain: coli, staphylococcus aureus and streptococcus agalactiae;
(3) Test strain preparation: activating and inoculating the test strain into LB liquid medium, culturing for 18-24 hr to obtain 1×10 strain 6 CFU/ml was ready for use.
(4) The test method comprises the following steps: preparing a sterilizing common agar culture medium plate according to a conventional method, and respectively inoculating different test strains on different plates; punching (3 mm) the inoculated strain plate with a puncher, injecting 25 μl of medicinal liquid (spray provided in examples 1-13 and comparative examples 1-10, blank group without medication) into each hole, culturing at 37deg.C for 24 hr, observing and recording the diameter (mm) of each bacteriostasis zone of each medicine, and the result is shown in Table 1;
TABLE 1
Drug group number | Coli (mm) | Staphylococcus aureus (mm) | Streptococcus agalactiae (mm) |
Example 1 | 9 | 8 | 8 |
Example 2 | 11 | 13 | 11 |
Example 3 | 12 | 12 | 12 |
Example 4 | 12 | 12 | 10 |
Example 5 | 11 | 12 | 11 |
Example 6 | 12 | 13 | 13 |
Example 7 | 11 | 12 | 13 |
Example 8 | 10 | 12 | 12 |
Example 9 | 11 | 11 | 12 |
Example 10 | 10 | 11 | 12 |
Example 11 | 10 | 12 | 11 |
Example 12 | 11 | 10 | 11 |
Comparative example 1 | 9 | 8 | 8 |
Comparative example 2 | 9 | 8 | 9 |
Comparative example 3 | 8 | 7 | 7 |
Comparative example 4 | 7 | 8 | 7 |
Comparative example 5 | 5 | 5 | 5 |
Comparative example 6 | 5 | 5 | 6 |
Comparative example 7 | 8 | 7 | 8 |
Comparative example 8 | 8 | 8 | 8 |
Comparative example 9 | 2 | 1 | 1 |
Blank group | 0 | 0 | 0 |
The data in Table 1 show that the composition prepared by the preparation method of the embodiment of the application has excellent antibacterial capability on escherichia coli, staphylococcus aureus and streptococcus agalactiae.
Experimental example 2 animal experiment
230 cows identified as having recessive mastitis were selected, wherein the condition of the cow udder is: the breast is slightly red and swollen, the infected milk area is hardened, and curd blocks or purulent secretion are arranged in the curd juice.
The cows are randomly divided into 23 experimental groups of 10 cows each; experiment groups 1-12 the compositions of examples 1-12 were sprayed on the surface of the dairy cow breast, respectively; the compositions of comparative examples 1-9 were sprayed on the udder surface of cows, respectively, in experimental groups 13-21; experimental group 22 was a blank control group, which was not subjected to any treatment; the treatment of experimental group 23 was performed by using the traditional method of injecting penicillin and streptomycin into the dairy room in combination with oral dandelion powder and magnesium sulfate solution to compress the affected part of the breast, and the cure rate (calculated by taking the disappearance of the symptoms as a cure standard and the cure rate as the cure standard) after 3 days of administration (administration twice a day is administration after the milk area of the affected cow is cleaned, and the dosage of the experimental group is the same) is counted, and the result is shown in table 2:
TABLE 2
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As can be seen from the data in table 2, the composition provided in examples 1 to 12 of the present application has an excellent effect of treating cow papilloma, and the cure rate in 3 days can be up to 97% or more, which indicates that the composition prepared by the preparation method provided in the present application can greatly shorten the treatment duration and has a remarkable treatment effect.
The lower cure rates of experimental groups 13 and 14 (corresponding to comparative examples 1 and 2) compared to experimental groups 2-12 demonstrate that the various process parameters in the preparation of the pearl dew grass extract of the present application can be synergistically enhanced to provide higher levels of active ingredients in the resulting pearl dew grass extract. The lower cure rates of experimental groups 15 and 16 (corresponding to comparative examples 3 and 4) compared to experimental groups 1-12 demonstrate that the various process parameters in the preparation of the American cockroach extract of the present application can synergistically cooperate to increase the active ingredient content of the resulting American cockroach extract. The lower cure rates of experimental groups 17 and 18 (corresponding to comparative examples 5 and 6) compared with experimental groups 1-12 indicate that the synergistic interaction can be achieved in the ratio range of the pearl water weed extract and the American cockroach extract provided by the application, so that the effect of the obtained composition for treating cow papillitis is better. The lower cure rates of experimental groups 19 and 20 (corresponding to comparative examples 7 and 8) compared to experimental groups 1-12 demonstrate the synergistic interaction within the process parameters of the preparation of the compositions provided herein, so that the resulting compositions have a better effect in treating cow papilloma. The lower cure rate of experimental group 21 (corresponding to comparative example 9) compared with experimental groups 1-12 indicates that the application adopts the pearl dew grass extract and the American cockroach extract to have a synergistic effect, and the prepared composition has a significantly better effect for treating cow mammitis than the effect of singly adopting the pearl dew grass extract.
In conclusion, the composition for treating the dairy cow mastitis provided by the invention has few raw material medicines, is short in time and remarkable in effect when being used for treating the dairy cow mastitis, has no toxic or side effect and is not easy to generate drug resistance. The preparation method of the composition for treating cow mastitis provided by the invention is simple and efficient, and is suitable for large-scale industrial production.
The above description is only of the preferred embodiments of the present invention and is not intended to limit the present invention, but various modifications and variations can be made to the present invention by those skilled in the art. Any modification, equivalent replacement, improvement, etc. made within the spirit and principle of the present invention should be included in the protection scope of the present invention.
Claims (6)
1. A composition for treating cow mastitis, comprising: the raw materials comprise, by weight, 10-20 parts of American cockroach extract and 8-13 parts of pearl water weed extract;
the preparation method of the composition comprises the following steps:
(1) The preparation of the periplaneta americana extract comprises the following steps: adding 1.5-2 times of water into the periplaneta americana coarse powder, performing flash extraction for 2-3 times under the condition of 92-108V for 2min each time to obtain an extracting solution, and performing centrifugal separation on the extracting solution to obtain the periplaneta americana extract;
(2) The preparation of the pearl water weed extract comprises the following steps: adding deionized water with the weight 7-9 times of that of the pearl dew grass into the pearl dew grass, boiling with strong fire, turning to low fire, continuously boiling for 30-40min, and filtering to obtain first filtrate and filter residue; adding deionized water with the weight 5-7 times of the weight of the filter residue into the filter residue, boiling with strong fire, and continuing boiling with small fire for 22-28min; filtering to obtain a second filtrate; combining the first filtrate and the second filtrate to obtain the pearl dew grass extract;
(3) Preparation of the composition: mixing the pearl dew grass extract and the American cockroach extract according to the weight parts to obtain a mixed solution, adding a natural clarifying agent into the mixed solution, stirring for 5-8min at the rotating speed of 600-800r/min, standing to remove grease and sediment, filtering and concentrating to obtain the composition.
2. The composition for treating cow mastitis according to claim 1, wherein: 13 parts of the periplaneta americana extract and 10 parts of the pearl water weed extract.
3. The composition for treating cow mastitis according to claim 2, wherein: in the step (2), the rotational speed of centrifugation is 3500-3800r/min, and the time is 30min.
4. A composition for treating bovine mastitis according to claim 3, wherein: the natural clarifying agent comprises one of chitosan, 101 fruit juice clarifying agent and gelatin.
5. The composition for treating bovine mastitis according to claim 4, wherein: the amount of the natural clarifying agent is 8-12% of the weight of the mixed solution.
6. The composition for treating cow mastitis according to claim 1, wherein: the formulation of the composition comprises one of ointment, spray, film coating agent and transdermal plaster.
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