CN115518026A

CN115518026A - External veterinary drug containing periplaneta americana as well as preparation method and application thereof

Info

Publication number: CN115518026A
Application number: CN202110712884.3A
Authority: CN
Inventors: 耿福能
Original assignee: Sichuan Gooddoctor Panxi Pharmaceutical Co ltd
Current assignee: Sichuan Gooddoctor Panxi Pharmaceutical Co ltd
Priority date: 2021-06-25
Filing date: 2021-06-25
Publication date: 2022-12-27

Abstract

The invention belongs to the technical field of veterinary drugs, and provides an external veterinary drug containing periplaneta americana as well as a preparation method and application thereof. The external veterinary drug containing the periplaneta americana comprises the following raw materials: periplaneta americana extract and algae extract. The external veterinary drug containing the periplaneta americana has few raw material types, can be used as a drug for treating the mastitis of the dairy cattle, and has the advantages of short treatment period, good effect, no drug resistance, no toxic or side effect and high safety. The preparation method comprises the following steps: mixing Periplaneta americana extract and algae extract, and adding glycerol. The preparation method is simple and efficient, and is suitable for industrial large-scale production.

Description

External veterinary drug containing periplaneta americana as well as preparation method and application thereof

Technical Field

The invention belongs to the technical field of veterinary drugs, and particularly relates to an external veterinary drug containing periplaneta americana as well as a preparation method and application thereof.

Background

Cow mastitis is an inflammatory reaction caused by stimulation of chemical and physical factors or invasion of various pathogenic microorganisms into cow breast tissues, is usually manifested as red, swollen, hot, painful breasts and is a common animal disease.

At present, antibiotic medicines such as penicillin, amoxicillin and cefuroxime are mostly adopted to treat cow mastitis. Antibiotics have certain effect on treating cow mastitis, however, long-term use of antibiotics can increase drug-resistant bacteria, and antibiotic residues easily cause 'milk resistance' and the like.

Patent document CN102488814B discloses a Chinese medicinal composition for treating cow mastitis, and specifically discloses a Chinese medicinal composition for treating cow mastitis, which comprises, by weight, 15-30 parts of root of red-rooted salvia, 5-10 parts of Chinese angelica, 1-5 parts of astragalus membranaceus, 5-10 parts of red peony root, 5-10 parts of corydalis tuber, 5-10 parts of rhizoma cyperi, 2-5 parts of branch shell, 5-10 parts of pericarpium citri reticulatae viride, 15-20 parts of honeysuckle, 5-10 parts of dandelion and 3-5 parts of liquorice. The composition can be prepared into paste, pulverizing the raw materials to obtain fine powders, mixing the fine powders to obtain mixed powder, adding edible vinegar and medical vaseline, and stirring to obtain paste. In the traditional Chinese medicine composition, the medicines are combined, and the traditional Chinese medicine composition has the functions of soothing the liver, removing blood stasis, regulating blood, stopping bleeding, promoting qi circulation, relieving pain, resisting bacteria, diminishing inflammation, tonifying qi, consolidating superficial resistance, supporting sores, promoting granulation, enhancing the immunity of the organism and the like. The Chinese patent medicine has obvious effect of treating and preventing the mastitis of the dairy cows, and has low cost and no toxic or side effect. Although the traditional Chinese medicine composition disclosed in the patent literature can avoid the problems of drug resistance and safety caused by the adoption of antibiotics, the required raw material medicines are various, the treatment period is long, and the treatment effect is general.

The periplaneta americana extract contains active substances such as WHF (polyhydric alcohols and epidermal growth factor), muconic acid, mucoglycine, various amino acids and the like, and has the effects of resisting inflammation, reducing swelling, promoting cell proliferation and growth of new granulation tissues, accelerating repair of damaged tissues, accelerating shedding of necrotic tissues, improving the immune function of an organism and the like. Can be clinically used for treating scald, burn and bedsore.

Algal plants are a group of lower plants which have chlorophyll, are in a vegetative life, have no substantial differentiation of roots, stems, leaves, and are bred with unicellular spores or zygotes. The algae can be widely used in food, medicine, fine chemical industry, feed and other industries. Patent No. CN103463139B discloses a preparation method of an anti-inflammatory, detumescent and antipruritic cream for skin extracted from microalgae meal, and further discloses that an external skin cream produced by using the microalgae meal extract as a raw material has the effects of resisting bacteria, relieving itching, diminishing inflammation and detumescence, and is suitable for local treatment of neurodermatitis, contact dermatitis, chronic eczema, insect bite dermatitis and pruritic skin diseases.

At present, the combination of the periplaneta americana extract and the algae extract as a medicament for treating the mastitis of the dairy cows has not been reported.

Disclosure of Invention

Aiming at the defects in the prior art, the first purpose of the invention is to provide the external veterinary drug containing the periplaneta americana, the external veterinary drug containing the periplaneta americana has few raw material types, can be used as a drug for treating the mastitis of the dairy cow, and has the advantages of short treatment period, good effect, no drug resistance, no toxic or side effect and high safety.

Aiming at the defects in the prior art, the second purpose of the invention is to provide a preparation method of the external veterinary drug containing periplaneta americana, which is simple and efficient and is suitable for industrial large-scale production.

In order to achieve the purpose, the invention adopts the following solution:

a topical veterinary drug containing Periplaneta americana comprises the following raw materials: periplaneta americana extract and algae extract.

A preparation method of the external veterinary drug containing the periplaneta americana comprises the following steps: mixing Periplaneta americana extract and algae extract, and adding glycerol.

An application of the external veterinary drug containing periplaneta americana in preparing a drug for treating cow mastitis.

The invention provides an external veterinary drug containing periplaneta americana, a preparation method and application thereof, and the external veterinary drug has the beneficial effects that:

(1) The external veterinary drug containing the periplaneta americana provided by the invention comprises the periplaneta americana extract and the algae extract. The main components of the periplaneta americana extract are peptides, alcohols, mucoglycine, mucoamino acid and other (18) amino acids, revived nucleosides, cardiovascular active peptides, high protein and newly discovered multiple growth-promoting factor groups. The periplaneta americana extract can remarkably promote the proliferation of the granulation tissue of the wound and the burn model and generate corresponding dose-effect relationship, and in addition, the periplaneta americana extract also has the effects of resisting virus, inflammation, oxidation, free radical and pain.

The algae include organisms of Chlorophyta, rhodophyta, dinophyta, xanthophyta, chrysophyta, diatoma, phaeophyta, and the like. The extract of algae is rich in protein, lipid, algal polysaccharides, inorganic elements, carotenoid and the like, and has high pharmacological and nutritional values.

The inventor unexpectedly finds that the periplaneta americana extract and the algae extract are combined to prepare the external veterinary drug containing the periplaneta americana, and the external veterinary drug can generate obvious synergistic effect when being used as a drug for treating the mastitis of the dairy cow, thereby not only greatly shortening the treatment period, but also having extremely high cure rate.

The external veterinary drug containing the periplaneta americana needs a few raw materials, the two raw materials are all pure natural animal and plant extracts, the drug is safe and environment-friendly, has no toxic or side effect, does not generate drug resistance, has very good synergistic effect, and has obvious treatment effect when being used as a cow mastitis drug.

(2) The preparation method of the external veterinary drug containing the periplaneta americana provided by the invention is simple and efficient, and is suitable for industrial large-scale production.

Detailed Description

In order to make the objects, technical solutions and advantages of the embodiments of the present invention clearer, the technical solutions in the embodiments of the present invention will be clearly and completely described below. The examples, in which specific conditions are not specified, were conducted under conventional conditions or conditions recommended by the manufacturer. The reagents or instruments used are not indicated by the manufacturer, and are all conventional products available commercially.

The external veterinary drug containing periplaneta americana provided by the embodiment of the invention and the preparation method and the application thereof are specifically explained below.

A topical veterinary drug containing Periplaneta americana comprises the following raw materials: periplaneta americana extract and algae extract. Further, the American cockroach extract and the alga extract are 6-9 parts by weight and 2-5 parts by weight. The inventor unexpectedly finds that the periplaneta americana extract and the algae extract have very excellent synergistic effect within the above proportioning range, the treatment period is short, the cure rate is high, and the recurrence rate is extremely low.

In this example, the preparation method of the periplaneta americana extract includes: crushing the dried periplaneta americana into coarse powder, adding 80-85% ethanol by volume, and performing reflux extraction to obtain an extracting solution. Filtering the extract to obtain filtrate. Adding natural clarifier into the filtrate, stirring for 5-8min, standing for 1-2 hr, removing oil and precipitate, filtering, and concentrating under reduced pressure to obtain Periplaneta americana extract. The periplaneta americana extract prepared by the preparation method is an external veterinary drug containing the periplaneta americana prepared by combining the periplaneta americana extract with the algae extract, and has a remarkable effect of treating the mastitis of the dairy cows.

In this embodiment, further, the algal plant is selected from chlorella, spirulina or dunaliella.

Wherein, chlorella belongs to Chlorophyta, which is a single-cell edible green alga, contains multiple vitamins, protein with the content as high as 70 percent and abundant micronutrient, and the content of carotene in the chlorella is 100 times of that in common plants. Chlorella has effects of improving immunity, reducing blood lipid, lowering blood pressure, preventing cancer, resisting cancer, regulating gastrointestinal absorption, promoting toxin excretion, protecting liver, removing toxic substance, treating cancer such as gastric ulcer and ulcerative colitis, and resisting bacteria and inflammation.

Spirulina belongs to the phylum cyanobacteria, is rich in nutrition, has protein content of 60% -70%, and also contains fat, carbohydrate, chlorophyll, carotenoid, phycocyanin, vitamins A, B1, B2, B6, B12, E, nicotinic acid, creatine, gamma-linolenic acid, calcium pantothenate, folic acid and calcium, ferrum, zinc, magnesium, etc. Spirulina has effects of reducing cholesterol, regulating blood sugar, enhancing immune system, protecting intestine and stomach, resisting tumor, preventing and inhibiting cancer, preventing and treating hyperlipidemia, resisting oxidation, resisting aging, relieving fatigue, resisting radiation, and treating anemia.

Dunaliella salina belongs to Chlorophyta, is rich in various natural carotenoids, folic acid, vitamin A, vitamin E, linolenic acid, linoleic acid, lecithin, fucoidin, dietary fiber and the like required by human health, more than 70 mineral substances and trace elements required by human body such as calcium, iron, zinc, selenium and the like, and is also rich in 18 amino acids, wherein 8 amino acids are necessary for human body. The Dunaliella salina can be used for preventing and adjuvant treating cancer, cardiovascular disease and cerebrovascular disease, stomach common diseases and oral ulcer, cataract, etc.

In this embodiment, the method for preparing the algae extract includes: collecting algae mud from the algae plant culture medium, cleaning the algae mud for 2 times, adding 85-92% ethanol with volume 3-5 times of that of the algae mud, performing ultrasonic treatment at 210-220W for 7-10min, performing constant temperature water bath at 40-46 deg.C in dark place for 10-15min, and filtering the filtrate to obtain algae extract. The alga extract prepared by the preparation method is combined with the periplaneta americana extract to prepare the periplaneta americana-containing external veterinary drug, and the effect of the external veterinary drug on treatment of cow mastitis is remarkable.

The embodiment also provides a preparation method of the external veterinary drug containing the periplaneta americana, which comprises the following steps: mixing the above Periplaneta americana extract and the above algae extract, and adding glycerol. In the embodiment, the external veterinary drug containing the periplaneta americana can be prepared into the following dosage forms according to the requirement and the conventional method: ointments, gels, solutions, aerosols, and the like.

Examples 1 to 3

The embodiment provides an external veterinary drug containing periplaneta americana, and the preparation method comprises the following steps: (1) preparing the periplaneta americana extract: crushing dried periplaneta americana into coarse powder, adding 82% ethanol by volume fraction, performing reflux extraction to obtain an extracting solution, filtering the extracting solution to obtain a filtrate, adding a natural clarifying agent into the filtrate, stirring for 7min, standing for 1.5h, removing oil and precipitates, filtering, and performing reduced pressure concentration to obtain the periplaneta americana extract; (2) preparation of algae extract: collecting algae mud from culture medium (Chlorella, spirulina, dunaliella salina), cleaning algae mud for 2 times, adding 90% ethanol 4 times volume of algae mud, performing ultrasonic treatment at 215W for 8min, extracting in 43 deg.C water bath under constant temperature and dark condition for 13min, and filtering to obtain filtrate to obtain algae extract; (3) preparation of external veterinary drug containing periplaneta americana: mixing the periplaneta americana extract (9 parts, 6 parts and 7 parts) and the algae extract (2 parts, 5 parts and 4 parts respectively) in parts by weight, and adding glycerol to obtain the compound feed.

Examples 4 to 5

The embodiment provides an external veterinary drug containing periplaneta americana, and the preparation method comprises the following steps: (1) preparing the periplaneta americana extract: crushing dried periplaneta americana into coarse powder, adding ethanol with volume fractions of 80% and 85% respectively, performing reflux extraction to obtain an extracting solution, filtering the extracting solution to obtain a filtrate, adding a natural clarifying agent into the filtrate, stirring for 5min and 8min respectively, standing for 1h and 2h respectively, removing grease and precipitates, filtering, and performing reduced pressure concentration to obtain a periplaneta americana extract; (2) preparation of algae extract: collecting algae mud from Dunaliella salina culture medium, cleaning algae mud for 2 times, adding 90% ethanol 4 times the volume of algae mud, performing ultrasonic treatment at 215W for 8min, performing constant temperature water bath at 43 deg.C in dark place for 13min, and filtering to obtain filtrate to obtain algae extract; (3) preparation of external veterinary drug containing periplaneta americana: respectively mixing 7 parts of periplaneta americana extract and 4 parts of algae extract according to the parts by weight, and adding glycerol to obtain the compound feed.

Examples 6 to 7

The embodiment provides an external veterinary drug containing periplaneta americana, and the preparation method comprises the following steps: (1) preparing the periplaneta americana extract: crushing dried periplaneta americana into coarse powder, adding 82% ethanol by volume fraction, performing reflux extraction to obtain an extracting solution, filtering the extracting solution to obtain a filtrate, adding a natural clarifying agent into the filtrate, stirring for 7min, standing for 1.5h, removing oil and precipitates, filtering, and performing reduced pressure concentration to obtain the periplaneta americana extract; (2) preparation of algae extract: collecting algae mud from Dunaliella salina plant culture medium, cleaning the algae mud for 2 times, respectively adding ethanol with the volume of 3 times and 5 times of the algae mud and the concentration of 85 percent and 92 percent into (210W and 220W) for ultrasonic treatment (7 min and 10 min), then extracting in constant temperature water bath at 40 ℃ and 46 ℃ in the dark for 10min and 15min, and filtering the obtained filtrate to obtain an algae extract; (3) preparation of external veterinary drug containing periplaneta americana: respectively mixing 7 parts of periplaneta americana extract and 4 parts of algae extract according to the parts by weight, and adding glycerol to obtain the compound feed.

Comparative example 1

The comparative example provides an external veterinary drug containing periplaneta americana, and the preparation method comprises the following steps: (1) preparing the periplaneta americana extract: crushing dried periplaneta americana into coarse powder, adding 82% ethanol by volume fraction, performing reflux extraction to obtain an extracting solution, filtering the extracting solution to obtain a filtrate, adding a natural clarifying agent into the filtrate, stirring for 7min, standing for 1.5h, removing oil and precipitates, filtering, and performing reduced pressure concentration to obtain the periplaneta americana extract; (2) preparation of external veterinary drug containing periplaneta americana: respectively adding 11 parts of periplaneta americana extract to glycerol according to the parts by weight to obtain the composition.

Comparative example 2

The comparative example provides an external veterinary drug containing periplaneta americana, and the preparation method comprises the following steps: (1) preparation of algae extract: collecting algae mud from Dunaliella salina culture medium, cleaning algae mud for 2 times, adding 90% ethanol 4 times the volume of algae mud, performing ultrasonic treatment at 215W for 8min, performing constant temperature water bath at 43 deg.C in dark place for 13min, and filtering to obtain filtrate to obtain algae extract; (2) preparation of external veterinary drug containing periplaneta americana: respectively adding 11 parts of the algae extract into glycerol according to the parts by weight to obtain the algae extract.

Comparative examples 3 to 4

The comparative example provides an external veterinary drug containing periplaneta americana, and the preparation method comprises the following steps: (1) preparing the periplaneta americana extract: crushing dried periplaneta americana into coarse powder, adding 82% ethanol by volume fraction, performing reflux extraction to obtain an extracting solution, filtering the extracting solution to obtain a filtrate, adding a natural clarifying agent into the filtrate, stirring for 7min, standing for 1.5h, removing oil and precipitates, filtering, and performing reduced pressure concentration to obtain the periplaneta americana extract; (2) preparation of algae extract: collecting algae mud from Dunaliella salina culture medium, cleaning algae mud for 2 times, adding 90% ethanol 4 times the volume of algae mud, performing ultrasonic treatment at 215W for 8min, performing constant temperature water bath and dark extraction at 43 deg.C for 13min, and filtering to obtain filtrate to obtain algae extract; (3) preparation of external veterinary drug containing periplaneta americana: mixing the periplaneta americana extract (10 parts and 5 parts) and the algae extract (1 part and 6 parts respectively) in parts by weight, and adding glycerol to obtain the compound feed.

Comparative example 5

The comparative example provides an external veterinary drug containing periplaneta americana, and the preparation method comprises the following steps: (1) preparing the periplaneta americana extract: crushing dried periplaneta americana into coarse powder, adding ethanol with the volume fraction of 95% for reflux extraction to obtain an extracting solution, filtering the extracting solution to obtain a filtrate, adding a natural clarifying agent into the filtrate, stirring for 10min, standing for 2.5h, removing grease and precipitates, filtering, and concentrating under reduced pressure to obtain the periplaneta americana extract; (2) preparation of algae extract: collecting algae mud from Dunaliella salina culture medium, cleaning algae mud for 2 times, adding 90% ethanol 4 times the volume of algae mud, performing ultrasonic treatment at 215W for 8min, performing constant temperature water bath and dark extraction at 43 deg.C for 13min, and filtering to obtain filtrate to obtain algae extract; (3) preparation of external veterinary drug containing periplaneta americana: respectively mixing 7 parts of periplaneta americana extract and 4 parts of algae extract according to the parts by weight, and adding glycerol to obtain the compound feed.

Comparative example 6

The comparative example provides an external veterinary drug containing periplaneta americana, and the preparation method comprises the following steps: (1) preparing the periplaneta americana extract: crushing dried periplaneta americana into coarse powder, adding 82% ethanol by volume fraction, performing reflux extraction to obtain an extracting solution, filtering the extracting solution to obtain a filtrate, adding a natural clarifying agent into the filtrate, stirring for 7min, standing for 1.5h, removing oil and precipitates, filtering, and performing reduced pressure concentration to obtain the periplaneta americana extract; (2) preparation of algae extract: collecting algae mud from Dunaliella salina plant culture medium, cleaning the algae mud for 2 times, adding 80% ethanol 6 times the volume of the algae mud, performing ultrasonic treatment at 230W for 6min, performing constant temperature water bath at 50 deg.C in dark place for 8min, and filtering the filtrate to obtain algae extract; (3) preparation of external veterinary drug containing periplaneta americana: respectively mixing 7 parts of periplaneta americana extract and 4 parts of algae extract according to the parts by weight, and adding glycerol to obtain the compound feed.

Experimental example 1 safety test of rabbits

The experimental method comprises the following steps: 26 adult healthy rabbits with the weight of 1.5 +/-0.2 Kg are taken and randomly divided into 13 groups, namely, male rabbits and female rabbits are divided into 13 groups, and after the skin of the rabbits is disinfected, the skin of the rabbits is rubbed by abrasive paper to obtain damaged skin experimental rabbits by the degree of bleeding. On the 2 nd day after the skin is damaged, the same amount of the external veterinary drug containing the periplaneta americana prepared in examples 1 to 7 and comparative examples 1 to 6 is respectively coated on the damaged area of the skin of the rabbit, and a layer of gauze is covered after the application and is fixed by a non-irritant adhesive plaster. Each animal was raised in cages. After the test object is given for 24 hours, the residual test object is removed by warm water or a non-irritant solvent, the body temperature, the changes of skin, hair, eyes and mucous membranes, respiration, central nervous system, limb activities and other toxic manifestations of the animal are observed and recorded 1 hour, 24 hours, 48 hours, 72 hours and 7 days after the test object is removed, and necropsy and visual observation should be carried out in time if the dead animal exists, and pathological histological examination should be carried out when pathological changes are visible to the naked eye.

The experimental results are as follows: after the external veterinary drug containing the periplaneta americana provided in the examples 1 to 7 and the comparative examples 1 to 6 is administered, no erythema or edema appears on the skin of the rabbits; the conditions of the rabbits of each group, such as ingestion, body temperature, skin, hair, eyes, mucous membrane, respiration, central nervous system, limb activity and the like, are normal, and no poisoning or abnormal symptom is seen, even no death condition occurs; it shows no acute skin toxicity and good safety.

Experimental example 2 animal clinical experiments

The test animals were selected from 300 clinical dairy cows with recessive mastitis in a dairy farm in Ganzizun. The condition of the cow udder is: the breast is slightly red and swollen, the affected breast area becomes hard, and there are clotted milk or purulent secretion in the coagulated milk.

Randomly dividing the cows into experimental groups 1-15, and each group has 20 cows; the externally applied veterinary drug containing periplaneta americana of examples 1-7 was smeared (2 mm thick, covering the affected milk area) on the affected milk areas of the cows of experimental groups 1-7 respectively; respectively smearing the same amount of the compositions of comparative examples 1-6 on the affected milk areas of the dairy cows of experimental groups 8-13; the cows of the experimental group 14 are treated by adopting a method of injecting penicillin and streptomycin into a breast room, combining oral dandelion powder and magnesium sulfate solution and carrying out hot compress on the affected part of the breast; experimental group 15 was a blank control group, which was not subjected to any treatment; the cure rate after 3 days of medication (twice a day, both the twice a day medication are the medication after the affected dairy cow breast areas are cleaned, and the dosage of each breast area of the experimental groups 1-13 is the same) is counted (the disappearance of the above symptoms is taken as the cure standard, and the cure rate is calculated: number of cured breast areas of cows in a group/number of affected breast areas of cows in a group 100%); and counting the recurrence rate (number of repeated cows in group/number of cows in group) of experimental groups 1-14 within half a year from the time of administration; the results are shown in Table 1:

TABLE 1

Group number	Cure rate	Recurrence rate
			1	99.8％	0
2	99.5％	0
			3	100％	0
4	99.4％	0
			5	99.6％	0
6	99.3％	0
			7	99.5％	0
8	85.2％	10％
			9	72.4％	15％
10	92.6％	0
			11	91.5％	0
12	88.4％	0
			13	86.1％	0
14	97.4％	10％
			15	0	-

The data in table 1 show that, compared with the traditional treatment method for treating cow mastitis in experimental example 14, the composition provided in examples 1 to 7 has a significant effect of treating cow mastitis, the cure rate of 3 days can reach more than 99%, and the recurrence rate within half a year is 0, which indicates that the external veterinary drug containing periplaneta americana prepared by the preparation method provided in the application can greatly shorten the treatment period, and the cure rate is extremely high and the recurrence rate is extremely low.

The periplaneta americana-containing veterinary drug for external use of the experimental group 8 (comparative example 1) is prepared by only the preparation method of examples 1 to 3 to obtain the periplaneta americana extract as the main raw material, and the amount of the periplaneta americana extract is the same as that of the total extract of examples 1 to 3. The results in table 1 show that the external veterinary drug containing periplaneta americana of experimental group 8, which is used as a therapeutic agent for cow mastitis, has a significantly reduced recovery rate within the same treatment time compared to examples 1 to 3, and has a recurrence within half a year.

The periplaneta americana-containing veterinary drug for external use of experimental group 9 (comparative example 2) was prepared using only the preparation methods of examples 1 to 3 to obtain the algae extract as the main raw material, and the amount of the algae extract was the same as that of the total extract of examples 1 to 3. The results in table 1 show that the external veterinary drug containing periplaneta americana of experimental group 9, which is used as a therapeutic agent for cow mastitis, has a significantly reduced recovery rate within the same treatment time compared to examples 1 to 3, and has a recurrence within half a year.

The periplaneta americana extract and the algae extract of experimental groups 10-11 (comparative examples 3-4) for external veterinary use containing periplaneta americana were prepared by the preparation methods of examples 1-3, but the ratio of the periplaneta americana extract to the algae extract is not within the protection scope of the present application. The results in table 1 show that the external veterinary drug containing periplaneta americana of experimental groups 10-11, which is used as the drug for treating cow mastitis, has a significantly reduced recovery rate in the same treatment time compared with examples 1-3, which indicates that the range of the ratio of the periplaneta americana extract to the algae extract in examples 1-3 of the present application is better for treating cow mastitis. The external veterinary drug containing the periplaneta americana of experimental groups 10-11 is adopted, no recurrence is caused within half a year, and the result proves that the external veterinary drug containing the periplaneta americana obtained by matching the periplaneta americana extract and the algae extract is thorough in treatment and good in stability and has certain repairing and preventing effects.

In the preparation process of the periplaneta americana-containing veterinary drug of experimental group 12 (comparative example 5), compared with examples 4-5, the preparation process parameters of the periplaneta americana extract are not within the protection scope of the present application under the same conditions. The results in table 1 show that the recovery rate of the periplaneta americana-containing veterinary drug of experimental group 12 as a treatment drug for cow mastitis is significantly reduced compared with that in examples 4-5 within the same treatment time, which indicates that the periplaneta americana extract prepared by the preparation process parameters of the periplaneta americana extract of the present application contains more effective components, and the periplaneta americana-containing veterinary drug prepared by the compatibility with the algae extract has a better treatment effect on cow mastitis. The external veterinary drug containing the periplaneta americana of the experimental group 12 has no recurrence within half a year, and proves that the external veterinary drug containing the periplaneta americana, which is obtained by matching the periplaneta americana extract and the algae extract, is thorough in treatment and good in stability, and has certain repairing and preventing effects.

In the preparation process of the periplaneta americana-containing veterinary drug of experimental group 13 (comparative example 6), the preparation process parameters of the algae extract are out of the protection scope of the present application compared to examples 6-7 under otherwise identical conditions. The results in table 1 show that the recovery rate of the external veterinary drug containing periplaneta americana of experimental group 13 as a treatment drug for cow mastitis is obviously reduced compared with that of examples 6 to 7 within the same treatment time, which indicates that the alga extract prepared by adopting the preparation process parameters of the alga extract of the application contains more effective components, and the external veterinary drug containing periplaneta americana prepared by matching with the periplaneta americana extract has better treatment effect on cow mastitis. The external veterinary drug containing the periplaneta americana of the experimental group 13 has no recurrence within half a year, and proves that the external veterinary drug containing the periplaneta americana, which is obtained by matching the periplaneta americana extract and the algae extract, is thorough in treatment and good in stability, and has certain repairing and preventing effects.

In conclusion, the external veterinary drug containing the periplaneta americana provided by the invention has the advantages of few raw material types, short treatment period, obvious effect, no toxic or side effect, high safety, thorough treatment and good stability as a drug for treating the mastitis of the dairy cow, and has certain repairing and preventing effects.

The above description is only a preferred embodiment of the present invention and is not intended to limit the present invention, and various modifications and changes may be made by those skilled in the art. Any modification, equivalent replacement, or improvement made within the spirit and principle of the present invention should be included in the protection scope of the present invention.

Claims

1. A topical veterinary drug containing Periplaneta americana is characterized in that: the raw materials comprise: periplaneta americana extract and algae extract.

2. The periplaneta americana-containing external veterinary drug according to claim 1, which is characterized in that: according to the parts by weight, the American cockroach extract is 6-9 parts, and the algae extract is 2-5 parts.

3. The periplaneta americana-containing topical veterinary drug according to claim 2, characterized in that: the preparation method of the periplaneta americana extract comprises the following steps: crushing the dried periplaneta americana into coarse powder, adding 80-85% ethanol by volume, and performing reflux extraction to obtain an extracting solution; filtering the extracting solution to obtain filtrate; adding a natural clarifying agent into the filtrate, stirring for 5-8min, standing for 1-2h, removing oil and precipitate, filtering, and concentrating under reduced pressure to obtain the Periplaneta americana extract.

4. The periplaneta americana-containing external veterinary drug according to claim 2, which is characterized by comprising the following components in percentage by weight: the preparation method of the algae extract comprises the following steps: collecting algae mud from algae plant culture medium, cleaning the algae mud for 2 times, adding ethanol with volume 3-5 times of the algae mud for ultrasonic treatment, performing light-shielding extraction in 40-46 deg.C water bath for 10-15min, and filtering the filtrate to obtain algae extract.

5. The periplaneta americana-containing external veterinary drug according to claim 4, which is characterized in that: the concentration of the ethanol is 85-92%.

6. The periplaneta americana-containing external veterinary drug according to claim 4, which is characterized in that: during ultrasonic treatment, the ultrasonic power is 210-220W, and the ultrasonic time is 7-10min.

7. The periplaneta americana-containing external veterinary drug according to claim 4, which is characterized in that: the algae comprises organisms of Chlorophyta, rhodophyta, dinophyta, xanthophyta, chrysophyta, diatom and Phaeophyta; preferably, the algal plant is selected from chlorella, spirulina or dunaliella.

8. The periplaneta americana-containing external veterinary drug according to claim 2, which is characterized by comprising the following components in percentage by weight: the external veterinary drug containing the periplaneta americana comprises an ointment, a gel, a solution and an aerosol.

9. The preparation method of the periplaneta americana-containing external veterinary drug as claimed in claim 1, is characterized by comprising the following steps: the method comprises the following steps: mixing the periplaneta americana extract and the algae extract, and adding glycerol to obtain the compound feed.

10. Use of the periplaneta americana-containing external veterinary drug as claimed in claim 1 in preparing a drug for treating cow mastitis.