CN114848680A - Composition for treating cow mastitis and preparation method thereof - Google Patents
Composition for treating cow mastitis and preparation method thereof Download PDFInfo
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Abstract
The invention belongs to the technical field of veterinary medicines, and provides a composition for treating cow mastitis and a preparation method thereof. The composition for treating cow mastitis comprises periplaneta americana extract as a raw material. The composition has the advantages of few kinds of raw material medicines, short time for treating cow mastitis, remarkable effect, no toxic or side effect and difficult generation of drug resistance. The preparation method comprises the following steps: (1) preparing the periplaneta americana extract; (2) and (3) preparing the composition. The preparation method is simple and efficient, and is suitable for large-scale industrial production.
Description
Technical Field
The invention belongs to the technical field of veterinary medicines, and particularly relates to a composition for treating cow mastitis and a preparation method thereof.
Background
Cow mastitis, also known as cow mastitis, is one of the most common diseases of cows, and is mostly caused by mechanical stimulation, invasion of pathogenic microorganisms and chemical and physical injuries. The mastitis of the dairy cattle not only affects the milk yield and causes economic loss, but also affects the quality of the milk and is harmful to human health. In summer, due to the influence of factors such as hot weather, humidity and poor sanitary conditions, mastitis is more likely to occur in dairy cows.
Cow mastitis is classified into serous mastitis, cellulose catarrhal mastitis, suppurative mastitis, hemorrhagic mastitis, gangrenous mastitis, and recessive mastitis. The main symptom set is represented by the following three aspects: (1) inflammation of the breast, such as redness, swelling, heat, pain, etc.; (2) reduced or stopped lactation; (3) the milk changes.
Cow mastitis is mainly caused by staphylococcus aureus, streptococcus and escherichia coli, and is usually treated by some antibiotics such as penicillin, gentamicin, lincomycin and the like.
Although the antibiotics take effect quickly, the antibiotics are easy to generate drug resistance, have high recurrence rate and are easy to leave chemical drugs in milk. In recent years, researches and clinical practices prove that the Chinese herbal medicine has great potential in the aspect of treating the mastitis of the dairy cows, has no toxic or side effect, and is not easy to generate drug resistance by pathogenic bacteria.
The invention patent with the patent number of CN102488814B provides a traditional Chinese medicine composition for treating cow mastitis, and particularly discloses that the traditional Chinese medicine composition for treating cow mastitis contains, by weight, 15-30 parts of raw material medicines of salvia miltiorrhiza, 5-10 parts of angelica sinensis, 1-5 parts of astragalus membranaceus, 5-10 parts of red peony roots, 5-10 parts of corydalis tuber, 5-10 parts of rhizoma cyperi, 2-5 parts of branch shells, 5-10 parts of green tangerine peels, 15-20 parts of honeysuckle flowers, 5-10 parts of dandelion and 3-5 parts of liquorice. The composition can be prepared into paste, pulverizing the raw materials to obtain fine powders, mixing the fine powders to obtain mixed powder, adding edible vinegar and medical vaseline, and stirring to obtain paste. In the traditional Chinese medicine composition, the medicines are combined, and the traditional Chinese medicine composition has the functions of soothing the liver, removing blood stasis, regulating blood, stopping bleeding, promoting qi circulation, relieving pain, resisting bacteria, diminishing inflammation, tonifying qi, consolidating superficial resistance, supporting sores, promoting granulation, enhancing the immunity of the organism and the like. The Chinese patent medicine has obvious effect of treating and preventing the mastitis of the dairy cows, and has low cost and no toxic or side effect. Although the traditional Chinese medicine composition has certain effect of treating and preventing the cow mastitis, the traditional Chinese medicine composition still has the defects of more adopted raw material medicines, poor drug effect and the like.
Disclosure of Invention
In view of the above-mentioned defects in the prior art, a first object of the present invention is to provide a composition for treating cow mastitis, wherein the composition has the advantages of few raw material drug types, short time, obvious effect, no toxic or side effect and no drug resistance.
In view of the above-mentioned disadvantages of the prior art, a second object of the present invention is to provide a method for preparing a composition for treating cow mastitis, which is simple and efficient, and is suitable for large-scale industrial production.
In order to achieve the above purpose, the solution adopted by the invention is as follows:
a composition for treating cow mastitis comprises Periplaneta americana extract.
A preparation method of the composition for treating cow mastitis comprises the following steps: (1) the preparation of the periplaneta americana extract: adding water 1.5-2 times the weight of Periplaneta americana coarse powder, performing flash extraction for 2-3 times under 92-108V for 2min each time to obtain extractive solution, and centrifuging to obtain Periplaneta americana extract
The composition for treating cow mastitis and the preparation method thereof provided by the invention have the beneficial effects that:
(1) the composition for treating cow mastitis provided by the invention comprises the following raw materials of periplaneta americana extract. The main components of the periplaneta americana extract are peptides (containing EGF), alcohols, mucoglycine, mucoamino acid and other (18) amino acids, revived nucleosides, cardiovascular active peptides, high protein and newly discovered multiple growth-promoting factor groups; the periplaneta americana extract can obviously promote the hyperplasia of wound surfaces and granulation tissues of burn models, and has a corresponding dose-effect relationship, and in addition, the periplaneta americana extract also has the effects of resisting virus, inflammation, oxidation, free radicals and pain. The inventor creatively discovers that the composition taking the periplaneta americana extract as the main raw material can greatly shorten the treatment time and has obvious treatment effect on the treatment of the cow mastitis; the periplaneta americana extract is safe and has no toxic or side effect because of natural components.
(2) According to the preparation method of the composition for treating cow mastitis provided by the invention, the periplaneta americana extract obtained by the preparation method is high in active ingredient content, and the preparation method is simple and efficient and is suitable for large-scale industrial production.
Detailed Description
In order to make the objects, technical solutions and advantages of the embodiments of the present invention clearer, the technical solutions in the embodiments of the present invention will be clearly and completely described below. The examples, in which specific conditions are not specified, were conducted under conventional conditions or conditions recommended by the manufacturer. The reagents or instruments used are not indicated by the manufacturer, and are all conventional products available commercially.
The following is a detailed description of a composition for treating cow mastitis and a preparation method thereof according to embodiments of the present invention.
The embodiment of the invention provides a composition for treating cow mastitis, which comprises the following raw materials of periplaneta americana extract and herba rhodiolae. Wherein, the American cockroach extract accounts for 10-20 parts by weight, and the margarita aquatic weed extract accounts for 8-13 parts by weight; further, 12-15 parts of periplaneta americana extract and 9-11 parts of herba rhodiolae extract; furthermore, the American cockroach extract is 13 parts, and the pearl dew grass extract is 10 parts.
Herba Cymbopogonis Citrari, also known as herba Chenopodii Hybridi, herba Cymbopogonis Citrari or herba Cymbopogonis Citrari, is root of plant Aranea grass belonging to Commelinaceae; herba Cymbopogonis Citrari has effects of dredging collaterals, relieving pain, promoting diuresis and relieving swelling; can be used for treating rheumatic arthralgia, lumbago, leg pain, numbness of limbs, edema, and eczema; the herba Eragrostidis Pendulifoliae extract contains beta-ecdysone as main ingredient, and the beta-ecdysone is one of polyhydroxy hormones, and has effects of promoting cell growth, stimulating dermal cell division, and promoting ecdysis.
The inventor creatively discovers that the periplaneta americana extract and the chrysopogon margaritifera extract can generate obvious synergistic interaction when being used in combination, can greatly shorten the treatment time on the treatment of the cow mastitis, and have obvious treatment effect; the periplaneta americana extract and the eriodictyon margarita extract are natural in components, so that the pharmaceutical composition is safe and has no toxic or side effect.
Within the range of the mixture ratio, the periplaneta Americana extract and the herba Corchori Mariani extract have excellent synergistic effect, can greatly shorten the treatment duration, and have remarkable treatment effect.
The embodiment of the invention also provides a preparation method of the composition for treating cow mastitis, which comprises the following steps: (1) the preparation of the periplaneta americana extract: adding water with the weight 1.5-2 times of the weight of the periplaneta Americana coarse powder, performing flash extraction for 2-3 times under the voltage of 92-108V for 2min each time to obtain an extracting solution, and performing centrifugal separation on the extracting solution for 30min at 3500-. In the step, all parameters are cooperated with one another, so that the American cockroach extract is rich in active substances.
(2) Preparing the herba rhodiolae extract: adding deionized water 7-9 times of the weight of herba Cymbopogonis Citrari, boiling with strong fire, decocting with slow fire for 30-40min, and filtering to obtain first filtrate and residue; adding deionized water 5-7 times of the weight of the residue, boiling with strong fire, and decocting with slow fire for 22-28 min; filtering to obtain a second filtrate; mixing the first filtrate and the second filtrate to obtain herba Cymbopogonis Citrari extract; in the step, in the two boiling and filtering processes, the proportion of the pearl dew grass and the deionized water can be matched with each other, and the boiling time can be matched with each other synergistically, so that the content of active ingredients in the filtrate can be increased.
(3) Preparation of the composition: mixing the herba Cordaeae Japonicae extract and Periplaneta americana extract according to weight parts to obtain a mixed solution, adding chitosan and 101 juice clarifier or gelatin with the weight of 8-12% of the mixed solution, stirring and mixing at the rotation speed of 600 plus materials of 800r/min for 5-8min, standing to remove grease and precipitate, filtering, and concentrating to obtain the composition. In the step, the proportion of the natural clarifying agent to the mixed liquid and the synergistic cooperation of other process parameters are adopted, so that the grease and impurities in the mixed liquid can be removed, and the purity of the mixed liquid is improved. In this embodiment, the formulation of the composition includes paste, spray, film coating agent and transdermal patch.
According to the preparation method, the periplaneta Americana extract and the herba Cymbopogonis Citrari extract are obtained respectively, and then are mixed and clarified, so that the effective components in the two extracts can be extracted to the maximum degree, and the purity of the final product can be improved. The preparation method is simple and efficient, and is suitable for large-scale industrial production.
The features and properties of the present invention are described in further detail below with reference to examples.
Example 1
The present embodiment provides a method for preparing a composition for treating bovine mastitis, comprising: (1) the preparation of the periplaneta americana extract: adding water 1.8 times the weight of Periplaneta americana coarse powder, performing flash extraction for 2 times under 100V voltage for 2min each time to obtain extractive solution, and centrifuging the extractive solution at 3600r/min for 30min to obtain Periplaneta americana extract; (2) preparation of the composition: adding 10% chitosan by weight into Periplaneta americana extract, stirring and mixing at 700r/min for 6min, standing to remove oil and precipitate, filtering, and concentrating to two-thirds of its volume to obtain spray composition.
Example 2
The present embodiment provides a method for preparing a composition for treating bovine mastitis, comprising:
(1) the preparation of the periplaneta americana extract: adding water 1.8 times the weight of Periplaneta americana coarse powder, performing flash extraction for 2 times under 100V voltage for 2min each time to obtain extractive solution, and centrifuging the extractive solution at 3600r/min for 30min to obtain Periplaneta americana extract;
(2) preparing the herba rhodiolae extract: adding deionized water 8 times of the weight of the pearl dew grass into the pearl dew grass, boiling the pearl dew grass with strong fire, then continuously boiling the pearl dew grass with soft fire for 35min, and filtering the pearl dew grass to obtain first filtrate and filter residue; adding deionized water 6 times of the weight of the filter residue, boiling with strong fire, and further boiling with slow fire for 25 min; filtering to obtain a second filtrate; mixing the first filtrate and the second filtrate to obtain herba Cymbopogonis Citrari extract;
(3) preparation of the composition: mixing 8 parts of the pearl dew grass extract and 20 parts of the periplaneta americana extract according to the parts by weight to obtain a mixed solution, adding 10% of chitosan of the weight of the mixed solution into the mixed solution, stirring and mixing the mixture for 6min at the rotating speed of 700r/min, standing the mixture to remove grease and sediment, filtering the mixture, and concentrating the mixture to two thirds of the volume of the mixture to prepare the spray composition.
Example 3
The present embodiment provides a method for preparing a composition for treating bovine mastitis, comprising:
(1) the preparation of the periplaneta americana extract: adding water 1.8 times the weight of Periplaneta americana coarse powder, performing flash extraction for 2 times under 100V voltage for 2min each time to obtain extractive solution, and centrifuging the extractive solution at 3600r/min for 30min to obtain Periplaneta americana extract;
(2) preparing the herba rhodiolae extract: adding deionized water 8 times of the weight of the pearl dew grass into the pearl dew grass, boiling the pearl dew grass with strong fire, then continuously boiling the pearl dew grass with soft fire for 35min, and filtering the pearl dew grass to obtain first filtrate and filter residue; adding deionized water 6 times of the weight of the filter residue, boiling with strong fire, and further boiling with slow fire for 25 min; filtering to obtain a second filtrate; mixing the first filtrate and the second filtrate to obtain herba Cymbopogonis Citrari extract;
(3) preparation of the composition: mixing 13 parts of the pearl dew grass extract and 10 parts of the periplaneta americana extract according to the parts by weight to obtain a mixed solution, adding 10% of 101 fruit juice clarifier of the weight of the mixed solution into the mixed solution, stirring and mixing the mixture for 6min at the rotating speed of 700r/min, standing the mixture to remove grease and sediment, filtering the mixture, and concentrating the mixture to two thirds of the volume of the mixture to obtain the spray composition.
Example 4
The present embodiment provides a method for preparing a composition for treating bovine mastitis, comprising:
(1) preparing the periplaneta americana extract: adding water 1.8 times the weight of Periplaneta americana coarse powder, performing flash extraction for 2 times under 100V voltage for 2min each time to obtain extractive solution, and centrifuging the extractive solution at 3600r/min for 30min to obtain Periplaneta americana extract;
(2) preparing the herba rhodiolae extract: adding deionized water 8 times of the weight of the pearl dew grass into the pearl dew grass, boiling the pearl dew grass with strong fire, then continuously boiling the pearl dew grass with soft fire for 35min, and filtering the pearl dew grass to obtain first filtrate and filter residue; adding deionized water 6 times of the weight of the filter residue, boiling with strong fire, and further boiling with slow fire for 25 min; filtering to obtain a second filtrate; mixing the first filtrate and the second filtrate to obtain herba Cymbopogonis Citrari extract;
(3) preparation of the composition: mixing 9 parts of the pearl dew grass extract and 15 parts of the periplaneta americana extract according to the parts by weight to obtain a mixed solution, adding 10% of gelatin of the weight of the mixed solution into the mixed solution, stirring and mixing the mixture for 6min at the rotating speed of 700r/min, standing the mixed solution to remove grease and sediment, filtering the mixed solution, and concentrating the filtered mixed solution to two thirds of the volume of the mixed solution to obtain the spray composition.
Example 5
The present embodiment provides a method for preparing a composition for treating bovine mastitis, comprising:
(1) the preparation of the periplaneta americana extract: adding water 1.8 times the weight of Periplaneta americana coarse powder, performing flash extraction for 2 times under 100V voltage for 2min each time to obtain extractive solution, and centrifuging the extractive solution at 3600r/min for 30min to obtain Periplaneta americana extract;
(2) preparing the herba rhodiolae extract: adding deionized water 8 times of the weight of the pearl dew grass into the pearl dew grass, boiling the pearl dew grass with strong fire, then continuously boiling the pearl dew grass with soft fire for 35min, and filtering the pearl dew grass to obtain first filtrate and filter residue; adding deionized water 6 times of the weight of the filter residue, boiling with strong fire, and further boiling with slow fire for 25 min; filtering to obtain a second filtrate; mixing the first filtrate and the second filtrate to obtain herba Cymbopogonis Citrari extract;
(3) preparation of the composition: mixing 11 parts of the pearl dew grass extract and 12 parts of the periplaneta americana extract according to the parts by weight to obtain a mixed solution, adding 10% of gelatin of the weight of the mixed solution into the mixed solution, stirring and mixing the mixture for 6min at the rotating speed of 700r/min, standing the mixed solution to remove grease and sediment, filtering the mixed solution, and concentrating the filtered mixed solution to two thirds of the volume of the mixed solution to obtain the spray composition.
Example 6
The present embodiment provides a method for preparing a composition for treating bovine mastitis, comprising:
(1) the preparation of the periplaneta americana extract: adding water with the weight 1.8 times of that of the periplaneta Americana coarse powder into the periplaneta Americana coarse powder, performing flash extraction for 2 times and 2min each time under the condition of 100V to obtain an extracting solution, and performing centrifugal separation on the extracting solution at 3600r/min for 30min to obtain the periplaneta Americana extract;
(2) preparing the herba rhodiolae extract: adding deionized water 8 times of the weight of the pearl dew grass into the pearl dew grass, boiling the pearl dew grass with strong fire, then continuously boiling the pearl dew grass with soft fire for 35min, and filtering the pearl dew grass to obtain first filtrate and filter residue; adding deionized water 6 times of the weight of the filter residue, boiling with strong fire, and further boiling with slow fire for 25 min; filtering to obtain a second filtrate; mixing the first filtrate and the second filtrate to obtain herba Cymbopogonis Citrari extract;
(3) preparation of the composition: mixing 13 parts of the pearl dew grass extract and 10 parts of the periplaneta americana extract according to the parts by weight to obtain a mixed solution, adding 10% of gelatin of the weight of the mixed solution into the mixed solution, stirring and mixing the mixture for 6min at the rotating speed of 700r/min, standing the mixed solution to remove grease and sediment, filtering the mixed solution, and concentrating the filtered mixed solution to two thirds of the volume of the mixed solution to obtain the spray composition.
Example 7
The present embodiment provides a method for preparing a composition for treating bovine mastitis, comprising:
(1) the preparation of the periplaneta americana extract: adding water 1.8 times the weight of Periplaneta americana coarse powder, performing flash extraction for 2 times under 100V voltage for 2min each time to obtain extractive solution, and centrifuging the extractive solution at 3600r/min for 30min to obtain Periplaneta americana extract;
(2) preparing the herba rhodiolae extract: adding deionized water 7 times of the weight of the pearl dew grass into the pearl dew grass, boiling with strong fire, then turning to small fire for continuously boiling for 30min, and filtering to obtain a first filtrate and filter residue; adding deionized water 5 times of the weight of the filter residue, boiling with strong fire, and further boiling with slow fire for 22 min; filtering to obtain a second filtrate; mixing the first filtrate and the second filtrate to obtain herba Cymbopogonis Citrari extract;
(3) preparation of the composition: mixing 13 parts of the pearl dew grass extract and 10 parts of the periplaneta americana extract according to the parts by weight to obtain a mixed solution, adding 10% of gelatin of the weight of the mixed solution into the mixed solution, stirring and mixing the mixture for 6min at the rotating speed of 700r/min, standing the mixed solution to remove grease and sediment, filtering the mixed solution, and concentrating the filtered mixed solution to two thirds of the volume of the mixed solution to obtain the spray composition.
Example 8
The present embodiment provides a method for preparing a composition for treating bovine mastitis, comprising:
(1) the preparation of the periplaneta americana extract: adding water 1.8 times the weight of Periplaneta americana coarse powder, performing flash extraction for 2 times under 100V voltage for 2min each time to obtain extractive solution, and centrifuging the extractive solution at 3600r/min for 30min to obtain Periplaneta americana extract;
(2) preparing the herba rhodiolae extract: adding deionized water 9 times of the weight of the pearl dew grass into the pearl dew grass, boiling with strong fire, then turning to small fire for continuously boiling for 40min, and filtering to obtain a first filtrate and filter residue; adding deionized water 7 times of the weight of the filter residue, boiling with strong fire, and further boiling with slow fire for 28 min; filtering to obtain a second filtrate; mixing the first filtrate and the second filtrate to obtain herba Cymbopogonis Citrari extract;
(3) preparation of the composition: mixing 13 parts of the pearl dew grass extract and 10 parts of the periplaneta americana extract according to the parts by weight to obtain a mixed solution, adding 10% of gelatin of the weight of the mixed solution into the mixed solution, stirring and mixing the mixture for 6min at the rotating speed of 700r/min, standing the mixed solution to remove grease and sediment, filtering the mixed solution, and concentrating the filtered mixed solution to two thirds of the volume of the mixed solution to obtain the spray composition.
Example 9
The present embodiment provides a method for preparing a composition for treating bovine mastitis, comprising:
(1) the preparation of the periplaneta americana extract: adding water 1.5 times the weight of Periplaneta americana coarse powder, performing flash extraction for 3 times under the voltage of 108V for 2min each time to obtain an extract, and performing centrifugal separation on the extract at 3500r/min for 30min to obtain a Periplaneta americana extract;
(2) preparing the herba rhodiolae extract: adding deionized water 8 times of the weight of the pearl dew grass into the pearl dew grass, boiling the pearl dew grass with strong fire, then continuously boiling the pearl dew grass with soft fire for 35min, and filtering the pearl dew grass to obtain first filtrate and filter residue; adding deionized water 6 times of the weight of the filter residue, boiling with strong fire, and further boiling with slow fire for 25 min; filtering to obtain a second filtrate; mixing the first filtrate and the second filtrate to obtain herba Hydrocotylis Parvifolii extract;
(3) preparation of the composition: mixing 13 parts of the pearl dew grass extract and 10 parts of the periplaneta americana extract according to the parts by weight to obtain a mixed solution, adding 10% of gelatin of the weight of the mixed solution into the mixed solution, stirring and mixing the mixture for 6min at the rotating speed of 700r/min, standing the mixed solution to remove grease and sediment, filtering the mixed solution, and concentrating the filtered mixed solution to two thirds of the volume of the mixed solution to obtain the spray composition.
Example 10
The present embodiment provides a method for preparing a composition for treating bovine mastitis, comprising:
(1) the preparation of the periplaneta americana extract: adding water 2 times the weight of Periplaneta americana coarse powder, performing flash extraction for 2 times under the voltage of 92V, 2min each time to obtain an extract, and performing centrifugal separation on the extract at 3800r/min for 30min to obtain a Periplaneta americana extract;
(2) preparing the herba rhodiolae extract: adding deionized water 8 times of the weight of the pearl dew grass into the pearl dew grass, boiling the pearl dew grass with strong fire, then continuously boiling the pearl dew grass with soft fire for 35min, and filtering the pearl dew grass to obtain first filtrate and filter residue; adding deionized water 6 times of the weight of the filter residue, boiling with strong fire, and further boiling with slow fire for 25 min; filtering to obtain a second filtrate; mixing the first filtrate and the second filtrate to obtain herba Cymbopogonis Citrari extract;
(3) preparation of the composition: mixing 13 parts of the pearl dew grass extract and 10 parts of the periplaneta americana extract according to the parts by weight to obtain a mixed solution, adding 10% of gelatin of the weight of the mixed solution into the mixed solution, stirring and mixing the mixture for 6min at the rotating speed of 700r/min, standing the mixed solution to remove grease and sediment, filtering the mixed solution, and concentrating the filtered mixed solution to two thirds of the volume of the mixed solution to obtain the spray composition.
Example 11
The present embodiment provides a method for preparing a composition for treating bovine mastitis, comprising:
(1) the preparation of the periplaneta americana extract: adding water 1.8 times the weight of Periplaneta americana coarse powder, performing flash extraction for 2 times under 100V voltage for 2min each time to obtain extractive solution, and centrifuging the extractive solution at 3600r/min for 30min to obtain Periplaneta americana extract;
(2) preparing the herba rhodiolae extract: adding deionized water 8 times of the weight of the pearl dew grass into the pearl dew grass, boiling the pearl dew grass with strong fire, then continuously boiling the pearl dew grass with soft fire for 35min, and filtering the pearl dew grass to obtain first filtrate and filter residue; adding deionized water 6 times of the weight of the filter residue, boiling with strong fire, and further boiling with slow fire for 25 min; filtering to obtain a second filtrate; mixing the first filtrate and the second filtrate to obtain herba Cymbopogonis Citrari extract;
(3) preparation of the composition: mixing 13 parts of the pearl dew grass extract and 10 parts of the periplaneta americana extract according to the parts by weight to obtain a mixed solution, adding 10% of gelatin of the weight of the mixed solution into the mixed solution, stirring and mixing the mixture for 8min at the rotating speed of 600r/min, standing the mixed solution to remove grease and sediment, filtering the mixed solution, and concentrating the filtered mixed solution to two thirds of the volume of the mixed solution to obtain the spray composition.
Example 12
The present embodiment provides a method for preparing a composition for treating bovine mastitis, comprising:
(1) the preparation of the periplaneta americana extract: adding water 1.8 times the weight of Periplaneta americana coarse powder, performing flash extraction for 2 times under 100V voltage for 2min each time to obtain extractive solution, and centrifuging the extractive solution at 3600r/min for 30min to obtain Periplaneta americana extract;
(2) preparing the herba rhodiolae extract: adding deionized water 8 times of the weight of the pearl dew grass into the pearl dew grass, boiling the pearl dew grass with strong fire, then continuously boiling the pearl dew grass with soft fire for 35min, and filtering the pearl dew grass to obtain first filtrate and filter residue; adding deionized water 6 times of the weight of the filter residue, boiling with strong fire, and further boiling with slow fire for 25 min; filtering to obtain a second filtrate; mixing the first filtrate and the second filtrate to obtain herba Cymbopogonis Citrari extract;
(3) preparation of the composition: mixing 13 parts of the pearl dew grass extract and 10 parts of the periplaneta americana extract according to the parts by weight to obtain a mixed solution, adding 10% of gelatin of the weight of the mixed solution into the mixed solution, stirring and mixing the mixture for 5min at the rotating speed of 800r/min, standing the mixed solution to remove grease and sediment, filtering the mixed solution, and concentrating the filtered mixed solution to two thirds of the volume of the mixed solution to obtain the spray composition.
Comparative example 1
The present comparative example provides a method of preparing a composition for treating bovine mastitis, comprising:
(1) the preparation of the periplaneta americana extract: adding water 1.8 times the weight of Periplaneta americana coarse powder, performing flash extraction for 2 times under 100V voltage for 2min each time to obtain extractive solution, and centrifuging the extractive solution at 3600r/min for 30min to obtain Periplaneta americana extract;
(2) preparing the herba rhodiolae extract: adding deionized water 6 times of the weight of the pearl dew grass into the pearl dew grass, boiling with strong fire, then continuing to boil with soft fire for 28min, and filtering to obtain a first filtrate and filter residue; adding deionized water 4 times of the weight of the filter residue, boiling with strong fire, and further boiling with slow fire for 20 min; filtering to obtain a second filtrate; mixing the first filtrate and the second filtrate to obtain herba Cymbopogonis Citrari extract;
(3) preparation of the composition: mixing 13 parts of the pearl dew grass extract and 10 parts of the periplaneta americana extract according to the parts by weight to obtain a mixed solution, adding 10% of gelatin of the weight of the mixed solution into the mixed solution, stirring and mixing the mixture for 6min at the rotating speed of 700r/min, standing the mixed solution to remove grease and sediment, filtering the mixed solution, and concentrating the filtered mixed solution to two thirds of the volume of the mixed solution to obtain the spray composition.
Comparative example 2
The present comparative example provides a method of preparing a composition for treating bovine mastitis, comprising:
(1) the preparation of the periplaneta americana extract: adding water 1.8 times the weight of Periplaneta americana coarse powder, performing flash extraction for 2 times under 100V voltage for 2min each time to obtain extractive solution, and centrifuging the extractive solution at 3600r/min for 30min to obtain Periplaneta americana extract;
(2) preparing the herba rhodiolae extract: adding 10 times of deionized water into herba Cymbopogonis Citrari, boiling with strong fire, decocting with slow fire for 42min, and filtering to obtain first filtrate and residue; adding deionized water 8 times of the weight of the filter residue, boiling with strong fire, and further boiling with slow fire for 30 min; filtering to obtain a second filtrate; mixing the first filtrate and the second filtrate to obtain herba Cymbopogonis Citrari extract;
(3) preparation of the composition: mixing 13 parts of the pearl dew grass extract and 10 parts of the periplaneta americana extract according to the parts by weight to obtain a mixed solution, adding 10% of gelatin of the weight of the mixed solution into the mixed solution, stirring and mixing the mixture for 6min at the rotating speed of 700r/min, standing the mixed solution to remove grease and sediment, filtering the mixed solution, and concentrating the filtered mixed solution to two thirds of the volume of the mixed solution to obtain the spray composition.
Comparative example 3
The present comparative example provides a method of preparing a composition for treating bovine mastitis, comprising:
(1) the preparation of the periplaneta americana extract: adding water 1.2 times the weight of Periplaneta americana coarse powder, performing flash extraction for 1 time and 3min each time under the condition of 110V voltage to obtain extractive solution, and performing centrifugal separation on the extractive solution at 3500r/min for 30min to obtain Periplaneta americana extract;
(2) preparing the herba rhodiolae extract: adding deionized water 8 times of the weight of the pearl dew grass into the pearl dew grass, boiling the pearl dew grass with strong fire, then continuously boiling the pearl dew grass with soft fire for 35min, and filtering the pearl dew grass to obtain first filtrate and filter residue; adding deionized water 6 times of the weight of the filter residue, boiling with strong fire, and further boiling with slow fire for 25 min; filtering to obtain a second filtrate; mixing the first filtrate and the second filtrate to obtain herba Cymbopogonis Citrari extract;
(3) preparation of the composition: mixing 13 parts of the pearl dew grass extract and 10 parts of the periplaneta americana extract according to the parts by weight to obtain a mixed solution, adding 10% of gelatin of the weight of the mixed solution into the mixed solution, stirring and mixing the mixture for 6min at the rotating speed of 700r/min, standing the mixed solution to remove grease and sediment, filtering the mixed solution, and concentrating the filtered mixed solution to two thirds of the volume of the mixed solution to obtain the spray composition.
Comparative example 4
The present comparative example provides a method of preparing a composition for treating bovine mastitis, comprising:
(1) the preparation of the periplaneta americana extract: adding water with the weight 3 times of that of the periplaneta Americana coarse powder into the periplaneta Americana coarse powder, performing flash extraction for 4 times under the condition of 90V voltage, wherein each time is 1min to obtain an extracting solution, and performing centrifugal separation on the extracting solution at 4000r/min for 30min to obtain the periplaneta Americana extract;
(2) preparing the herba rhodiolae extract: adding deionized water 8 times of the weight of the pearl dew grass into the pearl dew grass, boiling the pearl dew grass with strong fire, then continuously boiling the pearl dew grass with soft fire for 35min, and filtering the pearl dew grass to obtain first filtrate and filter residue; adding deionized water 6 times of the weight of the filter residue, boiling with strong fire, and further boiling with slow fire for 25 min; filtering to obtain a second filtrate; mixing the first filtrate and the second filtrate to obtain herba Cymbopogonis Citrari extract;
(3) preparation of the composition: mixing 13 parts of the pearl dew grass extract and 10 parts of the periplaneta americana extract according to the parts by weight to obtain a mixed solution, adding 10% of gelatin of the weight of the mixed solution into the mixed solution, stirring and mixing the mixture for 6min at the rotating speed of 700r/min, standing the mixed solution to remove grease and sediment, filtering the mixed solution, and concentrating the filtered mixed solution to two thirds of the volume of the mixed solution to obtain the spray composition.
Comparative example 5
The present comparative example provides a method of preparing a composition for treating bovine mastitis, comprising:
(1) the preparation of the periplaneta americana extract: adding water 1.8 times the weight of Periplaneta americana coarse powder, performing flash extraction for 2 times under 100V voltage for 2min each time to obtain extractive solution, and centrifuging the extractive solution at 3600r/min for 30min to obtain Periplaneta americana extract;
(2) preparing the herba rhodiolae extract: adding deionized water 8 times of the weight of the pearl dew grass into the pearl dew grass, boiling the pearl dew grass with strong fire, then continuously boiling the pearl dew grass with soft fire for 35min, and filtering the pearl dew grass to obtain first filtrate and filter residue; adding deionized water 6 times of the weight of the filter residue, boiling with strong fire, and further boiling with slow fire for 25 min; filtering to obtain a second filtrate; mixing the first filtrate and the second filtrate to obtain herba Cymbopogonis Citrari extract;
(3) preparation of the composition: mixing 14 parts of the pearl dew grass extract and 9 parts of the periplaneta americana extract according to the parts by weight to obtain a mixed solution, adding 10% of gelatin of the weight of the mixed solution into the mixed solution, stirring and mixing the mixture for 6min at the rotating speed of 700r/min, standing the mixed solution to remove grease and sediment, filtering the mixed solution, and concentrating the filtered mixed solution to two thirds of the volume of the mixed solution to obtain the spray composition.
Comparative example 6
The present comparative example provides a method of preparing a composition for treating bovine mastitis, comprising:
(1) the preparation of the periplaneta americana extract: adding water 1.8 times the weight of Periplaneta americana coarse powder, performing flash extraction for 2 times under 100V voltage for 2min each time to obtain extractive solution, and centrifuging the extractive solution at 3600r/min for 30min to obtain Periplaneta americana extract;
(2) preparing the herba rhodiolae extract: adding deionized water 8 times of the weight of the pearl dew grass into the pearl dew grass, boiling the pearl dew grass with strong fire, then continuously boiling the pearl dew grass with soft fire for 35min, and filtering the pearl dew grass to obtain first filtrate and filter residue; adding deionized water 6 times of the weight of the filter residue, boiling with strong fire, and further boiling with slow fire for 25 min; filtering to obtain a second filtrate; mixing the first filtrate and the second filtrate to obtain herba Cymbopogonis Citrari extract;
(3) preparation of the composition: mixing 7 parts of the pearl dew grass extract and 21 parts of the periplaneta americana extract according to the parts by weight to obtain a mixed solution, adding 10% of gelatin of the weight of the mixed solution into the mixed solution, stirring and mixing the mixture for 6min at the rotating speed of 700r/min, standing the mixed solution to remove grease and sediment, filtering the mixed solution, and concentrating the filtered mixed solution to two thirds of the volume of the mixed solution to obtain the spray composition.
Comparative example 7
The present comparative example provides a method of preparing a composition for treating bovine mastitis, comprising:
(1) the preparation of the periplaneta americana extract: adding water 1.8 times the weight of Periplaneta americana coarse powder, performing flash extraction for 2 times under 100V voltage for 2min each time to obtain extractive solution, and centrifuging the extractive solution at 3600r/min for 30min to obtain Periplaneta americana extract;
(2) preparing the herba rhodiolae extract: adding deionized water 8 times of the weight of the pearl dew grass into the pearl dew grass, boiling the pearl dew grass with strong fire, then continuously boiling the pearl dew grass with soft fire for 35min, and filtering the pearl dew grass to obtain first filtrate and filter residue; adding deionized water 6 times of the weight of the filter residue, boiling with strong fire, and further boiling with slow fire for 25 min; filtering to obtain a second filtrate; mixing the first filtrate and the second filtrate to obtain herba Cymbopogonis Citrari extract;
(3) preparation of the composition: mixing 13 parts of the pearl dew grass extract and 10 parts of the periplaneta americana extract according to the parts by weight to obtain a mixed solution, adding 7 percent of gelatin of the weight of the mixed solution into the mixed solution, stirring and mixing the mixture for 4min at the rotating speed of 900r/min, standing the mixed solution to remove grease and sediment, filtering the mixed solution, and concentrating the filtered mixed solution to two thirds of the volume of the mixed solution to obtain the spray composition.
Comparative example 8
The present comparative example provides a method of preparing a composition for treating bovine mastitis, comprising:
(1) the preparation of the periplaneta americana extract: adding water 1.8 times the weight of Periplaneta americana coarse powder, performing flash extraction for 2 times under 100V voltage for 2min each time to obtain extractive solution, and centrifuging the extractive solution at 3600r/min for 30min to obtain Periplaneta americana extract;
(2) preparing the herba rhodiolae extract: adding deionized water 8 times of the weight of the pearl dew grass into the pearl dew grass, boiling the pearl dew grass with strong fire, then continuously boiling the pearl dew grass with soft fire for 35min, and filtering the pearl dew grass to obtain first filtrate and filter residue; adding deionized water 6 times of the weight of the filter residue, boiling with strong fire, and further boiling with slow fire for 25 min; filtering to obtain a second filtrate; mixing the first filtrate and the second filtrate to obtain herba Cymbopogonis Citrari extract;
(3) preparation of the composition: mixing 13 parts of the pearl dew grass extract and 10 parts of the periplaneta americana extract according to the parts by weight to obtain a mixed solution, adding 13 percent of gelatin of the weight of the mixed solution into the mixed solution, stirring and mixing the mixture for 9min at the rotating speed of 500r/min, standing the mixed solution to remove grease and sediment, filtering the mixed solution, and concentrating the filtered mixed solution to two thirds of the volume of the mixed solution to obtain the spray composition.
Comparative example 9
The comparative example provides a preparation method of a margarita and aquatic weed extract paste, comprising the following steps: preparing the herba rhodiolae extract: adding deionized water 8 times of the weight of the pearl dew grass into the pearl dew grass, boiling the pearl dew grass with strong fire, then continuously boiling the pearl dew grass with soft fire for 35min, and filtering the pearl dew grass to obtain first filtrate and filter residue; adding deionized water 6 times of the weight of the filter residue, boiling with strong fire, and further boiling with slow fire for 25 min; filtering to obtain a second filtrate; mixing the first filtrate and the second filtrate to obtain herba Cymbopogonis Citrari extract; adding gelatin 10% of its weight into herba Cymbopogonis Citrari extract, stirring and mixing at 700r/min for 6min, standing to remove oil and precipitate, filtering, and concentrating to two-thirds of its volume to obtain herba Cymbopogonis Citrari extract spray.
Experimental example 1 in vitro bacteriostatic test for mastitis in milk cow
The experimental method comprises the following steps:
(1) main reagents and culture medium: beef extract, peptone, yeast powder, agar powder and common agar culture medium;
(2) test strains: escherichia coli, staphylococcus aureus, and bovine streptococcus agalactiae;
(3) preparation of test strains: activating and inoculating the test strain in LB liquid culture medium, culturing for 18-24h, and preparing into 1 × 10 6 CFU/ml for use.
(4) The test method comprises the following steps: preparing a sterilized common agar culture medium plate according to a conventional method, and respectively inoculating different test strains on different plates; punching holes (3mm) on the plate inoculated with the strain by using a puncher, injecting 25 μ l of liquid medicine (the spray provided by examples 1-13 and comparative examples 1-10, the blank group is not used with medicine) into each hole, culturing at 37 ℃ for 24h, observing and recording the size (mm) of the inhibition zone diameter of each medicine to each bacterium, and the result is shown in table 1;
TABLE 1
| Medicine group number | Escherichia coli (mm) | Staphylococcus aureus (mm) | Streptococcus agalactiae (mm) |
| Example 1 | 9 | 8 | 8 |
| Example 2 | 11 | 13 | 11 |
| Example 3 | 12 | 12 | 12 |
| Example 4 | 12 | 12 | 10 |
| Example 5 | 11 | 12 | 11 |
| Example 6 | 12 | 13 | 13 |
| Example 7 | 11 | 12 | 13 |
| Example 8 | 10 | 12 | 12 |
| Example 9 | 11 | 11 | 12 |
| Example 10 | 10 | 11 | 12 |
| Example 11 | 10 | 12 | 11 |
| Example 12 | 11 | 10 | 11 |
| Comparative example 1 | 9 | 8 | 8 |
| Comparative example 2 | 9 | 8 | 9 |
| Comparative example 3 | 8 | 7 | 7 |
| Comparative example 4 | 7 | 8 | 7 |
| Comparative example 5 | 5 | 5 | 5 |
| Comparative example 6 | 5 | 5 | 6 |
| Comparative example 7 | 8 | 7 | 8 |
| Comparative example 8 | 8 | 8 | 8 |
| Comparative example 9 | 2 | 1 | 1 |
| Blank group | 0 | 0 | 0 |
The data in table 1 show that the composition prepared by the preparation method of the embodiment of the application has excellent bacteriostatic ability on escherichia coli, staphylococcus aureus and bovine streptococcus agalactiae.
Experimental example 2 animal experiments
Selecting 230 cows identified as having subclinical mastitis, wherein the condition of the cow breasts is as follows: the breast is slightly red and swollen, the affected breast area becomes hard, and there are clotted milk or purulent secretion in the coagulated milk.
Randomly dividing the cows into 23 experimental groups, and 10 cows in each group; experimental groups 1-12 the compositions of examples 1-12 were sprayed onto the surface of a cow's udder, respectively; experimental groups 13-21 the compositions of comparative examples 1-9 were sprayed on the surface of cow udder, respectively; experimental group 22 was a blank control group, which was not subjected to any treatment; the experimental group 23 was treated by the traditional method of injecting penicillin and streptomycin into the breast chamber in combination with orally administered dandelion powder and magnesium sulfate solution for fomentation of the affected part of the breast, and the cure rate after 3 days of administration (twice daily administration, both administration after the affected cow breast areas were cleaned and the administration amount of the experimental group was the same) was counted (the cure rate was calculated as the cure standard, the cure rate was calculated as the number of cured breast areas of 10 cows/the number of affected breast areas of 10 cows: 100%), and the results are shown in table 2:
TABLE 2
As can be seen from the data in table 2, the compositions provided in examples 1 to 12 of the present application have excellent effect of treating bovine papillitis, and the cure rate of 3 days can be as high as 97% or more, which indicates that the compositions prepared by the preparation method provided in the present application can greatly shorten the treatment period and have significant treatment effect.
The cure rates of the experimental groups 13 and 14 (corresponding to the comparative examples 1 and 2) were lower than those of the experimental groups 2-12, which shows that the synergistic effect can be achieved within the process parameter ranges in the preparation of the extract of the herba rhodiolae so as to obtain the extract of the herba rhodiolae with higher active ingredient content. The cure rates of experimental groups 15 and 16 (corresponding to comparative examples 3 and 4) were lower than those of experimental groups 1-12, indicating that synergy can be achieved within the process parameter ranges in the preparation of the periplaneta americana extract of the present application, so that the contents of active ingredients in the obtained periplaneta americana extract are higher. The cure rate of the experimental groups 17 and 18 (corresponding to the comparative examples 5 and 6) is lower than that of the experimental groups 1 to 12, which shows that the pearl dew grass extract and the periplaneta americana extract provided by the application can be synergistically synergistic within the range of the mixture ratio, so that the obtained composition has better effect of treating the bovine papillitis. The lower cure rates of experimental groups 19 and 20 (corresponding to comparative examples 7 and 8) compared to experimental groups 1-12 indicate that synergistic synergy can be achieved within the process parameters in the preparation of the compositions provided herein, such that the resulting compositions are more effective in treating bovine papillitis. The cure rate of the experimental group 21 (corresponding to the comparative example 9) is lower than that of the experimental groups 1-12, which shows that the application adopts the herba rhodiolae extract and the periplaneta americana extract to have the synergistic effect, and the effect of the prepared composition for treating the cow papillitis is obviously better than that of the composition adopting the herba rhodiolae extract alone.
In conclusion, the composition for treating the cow mastitis provided by the invention has the advantages of few raw material medicine types, short time, obvious effect, no toxic or side effect and difficulty in generating drug resistance when being used for treating the cow mastitis. The preparation method of the composition for treating cow mastitis provided by the invention is simple and efficient, and is suitable for large-scale industrial production.
The above description is only a preferred embodiment of the present invention and is not intended to limit the present invention, and various modifications and changes may be made by those skilled in the art. Any modification, equivalent replacement, or improvement made within the spirit and principle of the present invention should be included in the protection scope of the present invention.
Claims (10)
1. A composition for treating bovine mastitis, comprising: the raw materials comprise American cockroach extracts.
2. The composition for use in the treatment of bovine mastitis according to claim 1, wherein: the raw materials also comprise a herba rhodiolae extract.
3. The composition for use in the treatment of bovine mastitis according to claim 2, wherein: according to the parts by weight, the American cockroach extract is 10-20 parts, and the margarita aquatic plant extract is 8-13 parts.
4. The composition for use in the treatment of bovine mastitis according to claim 3, wherein: according to the weight parts, the American cockroach extract is 13 parts, and the pearl dew grass extract is 10 parts.
5. A method of preparing a composition for treating bovine mastitis according to claims 1 to 4, wherein: the method comprises the following steps: (1) the preparation of the periplaneta americana extract: adding water with the weight 1.5-2 times of the weight of the periplaneta Americana coarse powder into the periplaneta Americana coarse powder, performing flash extraction for 2-3 times under the voltage of 92-108V for 2min each time to obtain an extracting solution, and performing centrifugal separation on the extracting solution to obtain the periplaneta Americana extract.
6. The method of preparing a composition for treating bovine mastitis according to claim 5, wherein: further comprising:
(2) preparing the herba rhodiolae extract: adding deionized water 7-9 times of the weight of herba Cymbopogonis Citrari, boiling with strong fire, decocting with slow fire for 30-40min, and filtering to obtain first filtrate and residue; adding deionized water 5-7 times of the weight of the filter residue, boiling with strong fire, and decocting with slow fire for 22-28 min; filtering to obtain a second filtrate; combining the first filtrate and the second filtrate to obtain the herba Hydrocotyles extract;
(3) preparation of the composition: mixing the herba rhodiolae extract and the periplaneta americana extract according to the weight part to obtain a mixed solution, adding a natural clarifying agent into the mixed solution, stirring at the rotation speed of 600-800r/min for 5-8min, standing to remove grease and precipitates, filtering, and concentrating to obtain the composition.
7. The method of preparing a composition for treating bovine mastitis according to claim 6, wherein: in the step (2), the rotation speed of the centrifugation is 3500-3800r/min, and the time is 30 min.
8. The method of preparing a composition for treating bovine mastitis according to claim 6, wherein: the natural clarifying agent comprises one of chitosan, 101 juice clarifying agent and gelatin.
9. The method of preparing a composition for treating bovine mastitis according to claim 8, wherein: the dosage of the natural clarifying agent is 8-12% of the self weight of the mixed solution.
10. The method of preparing a composition for treating bovine mastitis according to claim 6, wherein: the dosage forms of the composition comprise paste, spray, coating agent and transdermal plaster.
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Citations (12)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US20100254960A1 (en) * | 2007-10-03 | 2010-10-07 | E. I. Dupont Nemours And Company | Naphthalene isoxazoline compounds for control of invertebrate pests |
| CN102488814A (en) * | 2011-12-22 | 2012-06-13 | 中国农业科学院北京畜牧兽医研究所 | Chinese medicinal composition for treating bovine mastitis |
| CN102743741A (en) * | 2012-05-06 | 2012-10-24 | 大理学院 | Preparation of blattodea polypeptide, and uses of the same in anti-gram-positive bacteria and anti-gram-negative bacteria |
| CN103191232A (en) * | 2013-04-11 | 2013-07-10 | 西南大学 | Traditional Chinese medicine composition for preventing and treating mastitis of dairy cattle and preparation and application of composition |
| CN104622912A (en) * | 2015-03-05 | 2015-05-20 | 济南康众医药科技开发有限公司 | Pharmaceutical composition free of periplaneta Americana odor |
| CN105012442A (en) * | 2015-08-11 | 2015-11-04 | 黄春荣 | External traditional Chinese medicine composition for treating otitis media |
| CN105770779A (en) * | 2016-06-02 | 2016-07-20 | 烟台民望机电技术有限公司 | Medicine composition for preventing and treating contagious bovine pleuropneumonia and preparation method thereof |
| CN105878290A (en) * | 2015-07-06 | 2016-08-24 | 四川好医生攀西药业有限责任公司 | Periplaneta americana preparation and applications thereof in preparing medicines for treating colon cancer |
| CN106619767A (en) * | 2016-12-30 | 2017-05-10 | 成都乾坤动物药业股份有限公司 | Medicine composition for treating dairy cow mastitis as well as preparation method and application thereof |
| CN107412124A (en) * | 2017-07-22 | 2017-12-01 | 深圳诺漫斯生物科技有限公司 | A kind of anti-seborrhea shampoo of the active component containing American cockroach |
| CN114588130A (en) * | 2020-11-20 | 2022-06-07 | 四川好医生攀西药业有限责任公司 | Adhesive bandage containing periplaneta americana active ingredients and preparation method thereof |
| CN115518026A (en) * | 2021-06-25 | 2022-12-27 | 四川好医生攀西药业有限责任公司 | External veterinary drug containing periplaneta americana as well as preparation method and application thereof |
-
2021
- 2021-02-03 CN CN202110149441.8A patent/CN114848680B/en active Active
Patent Citations (12)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US20100254960A1 (en) * | 2007-10-03 | 2010-10-07 | E. I. Dupont Nemours And Company | Naphthalene isoxazoline compounds for control of invertebrate pests |
| CN102488814A (en) * | 2011-12-22 | 2012-06-13 | 中国农业科学院北京畜牧兽医研究所 | Chinese medicinal composition for treating bovine mastitis |
| CN102743741A (en) * | 2012-05-06 | 2012-10-24 | 大理学院 | Preparation of blattodea polypeptide, and uses of the same in anti-gram-positive bacteria and anti-gram-negative bacteria |
| CN103191232A (en) * | 2013-04-11 | 2013-07-10 | 西南大学 | Traditional Chinese medicine composition for preventing and treating mastitis of dairy cattle and preparation and application of composition |
| CN104622912A (en) * | 2015-03-05 | 2015-05-20 | 济南康众医药科技开发有限公司 | Pharmaceutical composition free of periplaneta Americana odor |
| CN105878290A (en) * | 2015-07-06 | 2016-08-24 | 四川好医生攀西药业有限责任公司 | Periplaneta americana preparation and applications thereof in preparing medicines for treating colon cancer |
| CN105012442A (en) * | 2015-08-11 | 2015-11-04 | 黄春荣 | External traditional Chinese medicine composition for treating otitis media |
| CN105770779A (en) * | 2016-06-02 | 2016-07-20 | 烟台民望机电技术有限公司 | Medicine composition for preventing and treating contagious bovine pleuropneumonia and preparation method thereof |
| CN106619767A (en) * | 2016-12-30 | 2017-05-10 | 成都乾坤动物药业股份有限公司 | Medicine composition for treating dairy cow mastitis as well as preparation method and application thereof |
| CN107412124A (en) * | 2017-07-22 | 2017-12-01 | 深圳诺漫斯生物科技有限公司 | A kind of anti-seborrhea shampoo of the active component containing American cockroach |
| CN114588130A (en) * | 2020-11-20 | 2022-06-07 | 四川好医生攀西药业有限责任公司 | Adhesive bandage containing periplaneta americana active ingredients and preparation method thereof |
| CN115518026A (en) * | 2021-06-25 | 2022-12-27 | 四川好医生攀西药业有限责任公司 | External veterinary drug containing periplaneta americana as well as preparation method and application thereof |
Non-Patent Citations (3)
| Title |
|---|
| HUANG T等: "DNA vaccination based on pyolysin co-immunized with IL-1β enhances host antibacterial immunity against Trueperella pyogenes infection", 《VACCINE》, vol. 2020, no. 30, pages 3469 - 3477 * |
| HUANG T等: "Quorum-sensing molecules N-acyl homoserine lactones inhibit Trueperella pyogenes infection in mouse model", 《VET MICROBIOL》, vol. 213, no. 2018, pages 89 - 94 * |
| 高洁等: "美洲大蠊药理作用及其临床疗效的研究进展", 中药药理与临床, vol. 34, no. 04, pages 205 - 210 * |
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