CN108042610B - Safe dental implant restoration liquid and preparation method thereof - Google Patents
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Abstract
The invention discloses a safe dental implant restoration liquid and a preparation method thereof, wherein the safe dental implant restoration liquid comprises the following raw materials in parts by weight: 60-80 parts of water, 20-30 parts of traditional Chinese medicine extract, 10-20 parts of pearl extract, 1-5 parts of repair synergist and 5-10 parts of honey. The safe dental implant restoration liquid and the preparation method thereof can effectively reduce postoperative infection and swelling, reduce wounds to the minimum, reduce inflammation, accelerate dental implant restoration, improve the success rate of dental implant implantation and prolong the service life of dental implant and natural tooth.
Description
Technical Field
The invention relates to the technical field of oral care, in particular to a safe dental implant restoration liquid and a preparation method thereof.
Background
The implant refers to a tooth missing restoration method for supporting and retaining an upper dental restoration based on a lower structure implanted into bone tissue. The dental implant comprises a lower supporting implant (dental implant) and an upper dental prosthesis (dental prosthesis-supported). It adopts artificial material (such as metal, ceramic, etc.) to make implant (generally similar to tooth root form), and adopts the operation method to implant into tissue (generally upper and lower jaws) and obtain firm retention support of bone tissue, and utilizes special device and mode to connect and support the dental prosthesis of upper portion.
The implant is the most advanced method for repairing the lost tooth because of the loss of tooth or dentition caused by periodontal disease, caries, trauma and the like. Through years of research and practice, the technology of the dental implant is mature, but due to the influence of various factors, the local part of the dental implant is easy to generate inflammatory reaction, the healing of the dental implant and surrounding bone tissues is influenced, the dental implant and the surrounding bone tissues are taken as main reasons for bone tissue absorption, and finally the dental implant fails. One important cause of implant failure is peri-implantitis, which is an inflammatory reaction that occurs in tissue surrounding an implant that has formed osseointegration and begins to function. Periimplantitis belongs to periodontitis in a wide sense, periodontal soft tissue is red, swollen and painful at the initial stage, periodontal pockets are formed to cause the loss and looseness of the implanted tooth level, and finally the failure of the implanted tooth is caused.
Therefore, the dental implant restoration care is very important. The invention aims to provide a safe dental implant restoration solution, which reduces postoperative infection and swelling, minimizes trauma, reduces inflammation and improves the success rate of dental implant.
Disclosure of Invention
Aiming at the defects in the prior art, the technical problem to be solved by the invention is to provide the safe dental implant restoration liquid and the preparation method thereof, and the obtained safe dental implant restoration liquid can effectively reduce postoperative infection and swelling, reduce the wound to the minimum degree, reduce the occurrence of inflammation, accelerate the restoration of a dental implant, improve the success rate of the dental implant and prolong the service life of the dental implant and natural teeth.
The purpose of the invention is realized by the following technical scheme:
a safe dental implant restoration liquid comprises the following raw materials in parts by weight: 60-80 parts of water, 20-30 parts of traditional Chinese medicine extract, 10-20 parts of pearl extract, 1-5 parts of repair synergist and 5-10 parts of honey.
A safe dental implant restoration liquid comprises the following raw materials in parts by weight: 60-80 parts of medical stone water, 20-30 parts of traditional Chinese medicine extract, 10-20 parts of pearl extract, 1-5 parts of repair synergist and 5-10 parts of honey.
The preparation method of the traditional Chinese medicine extracting solution comprises the following steps:
(1) drying flos Lonicerae 20-30 parts, Galla chinensis 20-30 parts, radix Arnebiae 5-10 parts, Scutellariae radix 5-10 parts, and radix Angelicae Dahuricae 1-5 parts at 60-80 deg.C for 6-10 hr respectively, pulverizing, and mixing to obtain Chinese medicinal powder;
(2) adding water 8-12 times the weight of the Chinese medicinal powder into the Chinese medicinal powder, maintaining the temperature at 90-98 deg.C for 50-60 min, filtering with 300 mesh filter cloth, and collecting filtrate A; adding water 5-10 times the weight of the Chinese medicinal powder into the residue, maintaining the temperature at 90-98 deg.C for 40-50 min, filtering with 300 mesh filter cloth to remove residue to obtain filtrate B, and mixing the two filtrates to obtain Chinese medicinal extractive solution.
The preparation method of the medical stone water comprises the following steps:
(1) cleaning the surface of medical stone, airing the surface moisture, crushing, and sieving by a 100-mesh sieve to obtain medical stone powder;
(2) mixing medical stone powder and water according to a solid-to-liquid ratio of 1 g: (10-20) mL, standing at 20-30 ℃ for 12-24 hours, and stirring at 60-80 ℃ for 2-6 hours at 300 rpm and 100-;
(3) centrifuging the Maifanitum mixed solution at 4000-.
Preferably, the preparation method of the medical stone water comprises the following steps:
(1) cleaning the surface of medical stone, airing the surface moisture, crushing, and sieving by a 100-mesh sieve to obtain medical stone powder;
(2) mixing medical stone powder and water according to a solid-to-liquid ratio of 1 g: (10-20) mL, standing at 20-30 ℃ for 12-24 hours, adjusting the pH to 5.5-6.5 with acid liquor, and stirring at 60-80 ℃ for 2-6 hours at 300 rpm under 100 ℃ to obtain medical stone mixed solution;
(3) centrifuging the Maifanitum mixed solution at 4000-.
The preparation method of the pearl extract comprises the following steps:
(1) cleaning Margarita, removing impurities, and pulverizing to obtain coarse Margarita powder;
(2) adding zirconia balls with the diameter of 0.1mm into a grinding cylinder of a sanding dispersion machine, adding ethanol into a feeding stirring cylinder, starting the grinding machine, adding coarse pearl powder simultaneously, wherein the solid-to-liquid ratio of the coarse pearl powder to the ethanol is 1 g: (15-25) mL, grinding for 65-75 minutes to obtain pearl powder slurry, centrifuging the pearl powder slurry for 10-20 minutes at 3000-5000 rpm/min, and drying the solid at 60-70 ℃ for 6-8 hours to obtain nano-scale pearl powder;
(3) nano-scale pearl powder and water are mixed according to the solid-to-liquid ratio of 1 g: (20-40) mL, adding mixed enzyme, wherein the addition amount of the mixed enzyme is 0.1-0.5% of the mass of the nano-scale pearl powder, stirring at 50-60 ℃ for 2-6 hours at 300 revolutions per minute, and preserving the temperature at 95-100 ℃ for 3-5 minutes to obtain enzymatic hydrolysate;
(4) centrifuging the enzymolysis solution at 3000-5000 rpm for 10-20 min, collecting supernatant, and filtering with membrane with pore diameter of 0.22 μm to obtain Margarita extractive solution.
The mixed enzyme is prepared by mixing 50-70 wt% of neutral protease and 30-50 wt% of papain.
The acid solution is 5-10% of organic acid aqueous solution, and the organic acid is one of citric acid, tartaric acid and lactic acid.
Preferably, the organic acid is lactic acid.
The repair synergist is lycium barbarum polysaccharide and/or soybean polysaccharide.
Preferably, the repair synergist is a mixture of lycium barbarum polysaccharide and soybean polysaccharide, and the mass ratio of the lycium barbarum polysaccharide to the soybean polysaccharide is (2-6): 1.
a preparation method of safe dental implant restoration liquid comprises the following steps: adding the traditional Chinese medicine extract, the pearl extract, the repair synergist and the honey into the medical stone water, and stirring for 30-60 minutes at 200-400 rpm to obtain the safe dental implant repair liquid.
The safe dental implant restoration liquid can be gargled, taken or smeared, and can be used before and after a dental implant operation.
The safe dental implant restoration liquid and the preparation method thereof can effectively reduce postoperative infection and swelling, reduce wounds to the minimum, reduce inflammation, accelerate dental implant restoration, improve the success rate of dental implant implantation and prolong the service life of dental implant and natural tooth.
Detailed Description
In the present invention, all the equipment and materials are commercially available or commonly used in the art, and the methods in the following examples are conventional in the art unless otherwise specified.
Pearl provided by Fuquan Zhenzhen Zhenjiang Fuzhen pearl farm is a substance secreted by Hyriopisi cumingii (Hyriopisi cumingii) under stimulation.
Maifanitum, and food grade Maifanitum provided by processing plant of Jinshou county mineral products.
Honeysuckle, Latin scientific name: lonicera Japonica, a dried bud or a flower with an initial bloom of Lonicera Japonica thunb, a honeysuckle family, and has a flavor: sweet and cold in nature. Meridian tropism: it enters lung, heart and stomach meridians. The efficacy is as follows: clear heat and remove toxicity, cool and disperse wind-heat. The main treatment is as follows: can be used for treating carbuncle, furuncle, pharyngitis, erysipelas, toxic heat, dysentery, wind-heat type common cold, epidemic febrile disease, and fever.
Gallnut, latin scientific name: rhus chinensis Mill, is Rhus chinensis Mill of Anacardiaceae, Rhus chinensis Mill, Rhus potaninii Maxim, or Rhus punjabensis Stew.var.sinica (Diels) Rehd.et wils. Nature and taste: sour, astringent and cold. Meridian tropism: it enters lung, large intestine and kidney meridians. The efficacy is as follows: astringe lung to reduce fire, astringe intestine to check diarrhea, astringe sweat to stop bleeding, astringe dampness to heal wound. The main treatment is as follows: can be used for treating chronic cough due to lung deficiency, chronic diarrhea, night sweat, diabetes, hemafecia, hemorrhoid, traumatic hemorrhage, carbuncle, sore, and skin ulcer.
Arnebia root, Latin scientific name: lithospermum erythrorhizon Sieb. et Zucc., which is a dried root of Arnebia euchroma (Royle) Johnst. or Arnebia guttata Bunge of Arnebia euchroma (Royle) of the family Boraginaceae. Nature and taste: sweet, salty and cold. Meridian tropism: it enters heart and liver meridians. The efficacy is as follows: cool blood, activate blood, remove toxicity and promote eruption. The main treatment is as follows: can be used for treating blood heat and toxic materials, purple and black skin, measles without adequate eruption, pyocutaneous disease, eczema, and scald due to hot water and fire.
Scutellaria, latin scientific name: scutellaria basilica Georgi, which is the dried root of Scutellaria baicalensis Georgi of Labiatae, and has the following properties and tastes: bitter and cold. Meridian tropism: it enters lung, gallbladder, spleen, large intestine and small intestine meridians. The efficacy is as follows: clear heat and dry dampness, purge toxicity, stop bleeding, prevent abortion. The main treatment is as follows: can be used for treating chest distress, nausea, damp-heat, dysentery, jaundice, cough due to lung heat, hyperpyrexia, polydipsia, hematemesis, carbuncle, swelling, sore, and threatened abortion.
Dahurian angelica root, Latin scientific name: angelica Dahurica (Fisch. ex Hoffm.) Benth.et Hook.f. ex Franch.et Sav is dried root of Angelica dahurica (Fisch. ex Hoffm.) Benth.et Hook.f. or Angelica dahurica (Fisch. ex Hoffm.) Benth.et Hoffm. Benth.f. or Angelica dahurica (Boiss.) Benth.et Hook.f. var.fortmosana (Boiss.) Shan et Yuan of Umbelliferae). Nature and taste: pungent and warm. Meridian tropism: it enters stomach, large intestine and lung meridians. The efficacy is as follows: dispel wind and dampness, induce resuscitation and alleviate pain, resolve swelling and expel pus. The main treatment is as follows: can be used for treating common cold, headache, supraorbital bone pain, nasal obstruction, nasosinusitis, toothache, leucorrhea, and pyocutaneous disease with swelling and pain.
The papain is food-grade papain produced by Biotech Limited of Henghuadao, Nanjing, with enzyme activity of 30000U/g.
The neutral protease is food-grade neutral protease produced by Biotech Limited of Henghuadao, Nanjing, with enzyme activity of 30000U/g.
Soybean polysaccharide, provided by Jiangsu Qianji bioengineering Co., Ltd, is in food grade.
Lycium barbarum polysaccharides, food grade, available from Walt Laisi Biotech, Lanzhou
Citric acid, CAS No.: 77-92-9, food-grade anhydrous citric acid provided by Weifang Yixuan industry Co., Ltd.
Tartaric acid, CAS number: 133-37-9, and food grade DL-tartaric acid produced by a new Hangzhou Linan Jinlong chemical company.
Lactic acid, CAS No.: 50-21-5, food grade lactic acid produced by Shandong Baisheng Biotech limited.
Honey, locust honey provided by the Yongxing cooperative society for honey breeding in Mengyin county.
The sanding dispersion machine adopts a vertical double-shaft nano ceramic sanding dispersion machine which is produced by Shenzhen Keli nano engineering equipment Limited and has the model of KLLN.
Ethanol, in the examples, absolute ethanol was used, and was supplied by Yonghua chemical technology (Jiangsu) GmbH.
Anti-inflammatory effect test: 150 SPF-grade healthy Kunming mice with the weight of 20 +/-2 g, the temperature of a breeding house of each half of male and female animals controlled at 20-25 ℃, the relative humidity controlled at 50-60%, good ventilation, artificial illumination for 12h and clean cages. Mice were fed water and adapted to the experimental environment for 3 days, and were fasted for 12h before the experiment.
The mice were randomly divided into 10 groups of 15 mice each, namely a control group and an experimental group (examples 1-7 and comparative examples 1-2). The experimental group is perfused with the safe dental implant restoration solution obtained by the invention once a day and continuously perfused with stomach for 5 days according to 0.1mL/10g of stomach, the contrast group is perfused with the same amount of normal saline, after the last drug administration for 2 hours, both sides of the right ear of each mouse are coated with 30 mu L of xylene inflammation causing agent (produced by Chongqing Chundong chemical industry), and the left ear is not treated as the contrast of the normal ear. After 30min of inflammation, the mice were sacrificed by dislocation of cervical vertebrae, both ears were cut along the baseline of pinna, the ears were removed from the same portions of both ears respectively by a punch with a diameter of 8mm, weighed on an analytical balance, respectively, and the inhibition rate of auricle swelling was calculated according to the following formula.
Statistical analysis is carried out after comparing the mouse ear corridor swelling test data, and the result shows that P is less than 0.05, and the difference has statistical significance.
The antibacterial performance of the safe dental implant restoration liquid obtained in the embodiment is tested by referring to the method of appendix C in GB15797-2002, and the test strains are Candida albicans (ATCC 10231) and Staphylococcus aureus (ATCC 6538).
Example 1
The safe dental implant restoration liquid comprises the following raw materials in parts by weight: 70 parts of water, 25 parts of traditional Chinese medicine extracting solution, 15 parts of pearl extracting solution, 2 parts of repair synergist and 8 parts of honey.
The preparation method of the traditional Chinese medicine extracting solution comprises the following steps:
(1) respectively drying 25 parts of honeysuckle, 25 parts of gallnut, 8 parts of lithospermum, 8 parts of scutellaria baicalensis and 3 parts of angelica dahurica at 70 ℃ for 8 hours, crushing, sieving with a 80-mesh sieve, and uniformly mixing to obtain traditional Chinese medicine powder;
(2) adding water 10 times the weight of the Chinese medicinal powder into the Chinese medicinal powder, keeping the temperature at 95 deg.C for 55 min, filtering with 300 mesh filter cloth, and collecting filtrate A; adding water 8 times the weight of the Chinese medicinal powder into the residue, keeping the temperature at 95 deg.C for 45 min, filtering with 300 mesh filter cloth to remove residue to obtain filtrate B, and mixing the two filtrates to obtain Chinese medicinal extractive solution.
The preparation method of the pearl extract comprises the following steps:
(1) cleaning Margarita, removing impurities, pulverizing, and sieving with 80 mesh sieve to obtain coarse Margarita powder;
(2) adding zirconia ball with diameter of 0.1mm into the grinding cylinder of sanding dispersion machine, adding ethanol into the feeding mixing cylinder, starting the grinding machine, rotating speed of the main machine is 1800 rpm, feeding pressure is 0.35kPa, adding crude pearl powder simultaneously, and the solid-to-liquid ratio of the crude pearl powder and the ethanol is 1 g: 20mL, the mass ratio of the crude pearl powder to the zirconia balls is 1: 30, grinding for 70 minutes to obtain pearl powder slurry, centrifuging the pearl powder slurry at 4000 rpm/separation for 15 minutes, and drying the solid at 65 ℃ for 7 hours to obtain nano-scale pearl powder;
(3) nano-scale pearl powder and water are mixed according to the solid-to-liquid ratio of 1 g: mixing 30mL of the mixed solution, adding mixed enzyme, wherein the adding amount of the mixed enzyme is 0.3 percent of the mass of the nano-scale pearl powder, stirring for 4 hours at the temperature of 55 ℃ at 200 rpm, and preserving the heat for 4 minutes at the temperature of 98 ℃ to obtain enzymatic hydrolysate;
(4) centrifuging the enzymolysis solution at 4000 rpm for 15 min, collecting supernatant, and filtering with membrane with pore diameter of 0.22 μm to obtain Margarita extractive solution.
The mixed enzyme is prepared by mixing 60 wt% of neutral protease and 40 wt% of papain.
The repair synergist is lycium barbarum polysaccharide.
The preparation method of the safe dental implant restoration liquid comprises the following steps: adding the traditional Chinese medicine extracting solution, the pearl extracting solution, the repair synergist and the honey into water, and stirring at 300 revolutions per minute for 40 minutes to obtain the safe dental implant repair solution.
Example 2
The safe dental implant restoration liquid comprises the following raw materials in parts by weight: 70 parts of medical stone water, 25 parts of traditional Chinese medicine extracting solution, 15 parts of pearl extracting solution, 2 parts of repair synergist and 8 parts of honey.
The preparation method of the traditional Chinese medicine extracting solution comprises the following steps:
(1) respectively drying 25 parts of honeysuckle, 25 parts of gallnut, 8 parts of lithospermum, 8 parts of scutellaria baicalensis and 3 parts of angelica dahurica at 70 ℃ for 8 hours, crushing, sieving with a 80-mesh sieve, and uniformly mixing to obtain traditional Chinese medicine powder;
(2) adding water 10 times the weight of the Chinese medicinal powder into the Chinese medicinal powder, keeping the temperature at 95 deg.C for 55 min, filtering with 300 mesh filter cloth, and collecting filtrate A; adding water 8 times the weight of the Chinese medicinal powder into the residue, keeping the temperature at 95 deg.C for 45 min, filtering with 300 mesh filter cloth to remove residue to obtain filtrate B, and mixing the two filtrates to obtain Chinese medicinal extractive solution.
The preparation method of the medical stone water comprises the following steps:
(1) cleaning the surface of medical stone, airing the surface moisture, crushing, and sieving by a 100-mesh sieve to obtain medical stone powder;
(2) mixing medical stone powder and water according to a solid-to-liquid ratio of 1 g: mixing 15mL of the components, standing the mixture for 18 hours at 25 ℃, and stirring the mixture for 4 hours at 70 ℃ at 200 rpm to obtain medical stone mixed solution;
(3) centrifuging the Maifanitum mixed solution at 6000 rpm for 15 min, collecting supernatant, and filtering with membrane with pore diameter of 0.22 μm to obtain Maifanitum water.
The preparation method of the pearl extract comprises the following steps:
(1) cleaning Margarita, removing impurities, pulverizing, and sieving with 80 mesh sieve to obtain coarse Margarita powder;
(2) adding zirconia ball with diameter of 0.1mm into the grinding cylinder of sanding dispersion machine, adding ethanol into the feeding mixing cylinder, starting the grinding machine, rotating speed of the main machine is 1800 rpm, feeding pressure is 0.35kPa, adding crude pearl powder simultaneously, and the solid-to-liquid ratio of the crude pearl powder and the ethanol is 1 g: 20mL, the mass ratio of the crude pearl powder to the zirconia balls is 1: 30, grinding for 70 minutes to obtain pearl powder slurry, centrifuging the pearl powder slurry at 4000 rpm/separation for 15 minutes, and drying the solid at 65 ℃ for 7 hours to obtain nano-scale pearl powder;
(3) nano-scale pearl powder and water are mixed according to the solid-to-liquid ratio of 1 g: mixing 30mL of the mixed solution, adding mixed enzyme, wherein the adding amount of the mixed enzyme is 0.3 percent of the mass of the nano-scale pearl powder, stirring for 4 hours at the temperature of 55 ℃ at 200 rpm, and preserving the heat for 4 minutes at the temperature of 98 ℃ to obtain enzymatic hydrolysate;
(4) centrifuging the enzymolysis solution at 4000 rpm for 15 min, collecting supernatant, and filtering with membrane with pore diameter of 0.22 μm to obtain Margarita extractive solution.
The mixed enzyme is prepared by mixing 60 wt% of neutral protease and 40 wt% of papain.
The repair synergist is lycium barbarum polysaccharide.
The preparation method of the safe dental implant restoration liquid comprises the following steps: adding the traditional Chinese medicine extract, the pearl extract, the repair synergist and the honey into the medical stone water, and stirring at 300 revolutions per minute for 40 minutes to obtain the safe dental implant repair liquid.
Comparative example 1
The safe dental implant restoration liquid comprises the following raw materials in parts by weight: 85 parts of medical stone water, 25 parts of traditional Chinese medicine extracting solution, 2 parts of repair synergist and 8 parts of honey.
The preparation method of the traditional Chinese medicine extracting solution comprises the following steps:
(1) respectively drying 25 parts of honeysuckle, 25 parts of gallnut, 8 parts of lithospermum, 8 parts of scutellaria baicalensis and 3 parts of angelica dahurica at 70 ℃ for 8 hours, crushing, sieving with a 80-mesh sieve, and uniformly mixing to obtain traditional Chinese medicine powder;
(2) adding water 10 times the weight of the Chinese medicinal powder into the Chinese medicinal powder, keeping the temperature at 95 deg.C for 55 min, filtering with 300 mesh filter cloth, and collecting filtrate A; adding water 8 times the weight of the Chinese medicinal powder into the residue, keeping the temperature at 95 deg.C for 45 min, filtering with 300 mesh filter cloth to remove residue to obtain filtrate B, and mixing the two filtrates to obtain Chinese medicinal extractive solution.
The preparation method of the medical stone water comprises the following steps:
(1) cleaning the surface of medical stone, airing the surface moisture, crushing, and sieving by a 100-mesh sieve to obtain medical stone powder;
(2) mixing medical stone powder and water according to a solid-to-liquid ratio of 1 g: mixing 15mL of the components, standing the mixture for 18 hours at 25 ℃, and stirring the mixture for 4 hours at 70 ℃ at 200 rpm to obtain medical stone mixed solution;
(3) centrifuging the Maifanitum mixed solution at 6000 rpm for 15 min, collecting supernatant, and filtering with membrane with pore diameter of 0.22 μm to obtain Maifanitum water.
The repair synergist is lycium barbarum polysaccharide.
The preparation method of the safe dental implant restoration liquid comprises the following steps: adding the traditional Chinese medicine extract, the repair synergist and the honey into the medical stone water, and stirring at 300 revolutions per minute for 40 minutes to obtain the safe dental implant repair liquid.
Comparative example 2
The safe dental implant restoration liquid comprises the following raw materials in parts by weight: 25 parts of traditional Chinese medicine extracting solution, 85 parts of pearl extracting solution, 2 parts of repair synergist and 8 parts of honey.
The preparation method of the traditional Chinese medicine extracting solution comprises the following steps:
(1) respectively drying 25 parts of honeysuckle, 25 parts of gallnut, 8 parts of lithospermum, 8 parts of scutellaria baicalensis and 3 parts of angelica dahurica at 70 ℃ for 8 hours, crushing, sieving with a 80-mesh sieve, and uniformly mixing to obtain traditional Chinese medicine powder;
(2) adding water 10 times the weight of the Chinese medicinal powder into the Chinese medicinal powder, keeping the temperature at 95 deg.C for 55 min, filtering with 300 mesh filter cloth, and collecting filtrate A; adding water 8 times the weight of the Chinese medicinal powder into the residue, keeping the temperature at 95 deg.C for 45 min, filtering with 300 mesh filter cloth to remove residue to obtain filtrate B, and mixing the two filtrates to obtain Chinese medicinal extractive solution.
The preparation method of the pearl extract comprises the following steps:
(1) cleaning Margarita, removing impurities, pulverizing, and sieving with 80 mesh sieve to obtain coarse Margarita powder;
(2) adding zirconia ball with diameter of 0.1mm into the grinding cylinder of sanding dispersion machine, adding ethanol into the feeding mixing cylinder, starting the grinding machine, rotating speed of the main machine is 1800 rpm, feeding pressure is 0.35kPa, adding crude pearl powder simultaneously, and the solid-to-liquid ratio of the crude pearl powder and the ethanol is 1 g: 20mL, the mass ratio of the crude pearl powder to the zirconia balls is 1: 30, grinding for 70 minutes to obtain pearl powder slurry, centrifuging the pearl powder slurry at 4000 rpm/separation for 15 minutes, and drying the solid at 65 ℃ for 7 hours to obtain nano-scale pearl powder;
(3) nano-scale pearl powder and water are mixed according to the solid-to-liquid ratio of 1 g: mixing 30mL of the mixed solution, adding mixed enzyme, wherein the adding amount of the mixed enzyme is 0.3 percent of the mass of the nano-scale pearl powder, stirring for 4 hours at the temperature of 55 ℃ at 200 rpm, and preserving the heat for 4 minutes at the temperature of 98 ℃ to obtain enzymatic hydrolysate;
(4) centrifuging the enzymolysis solution at 4000 rpm for 15 min, collecting supernatant, and filtering with membrane with pore diameter of 0.22 μm to obtain Margarita extractive solution.
The mixed enzyme is prepared by mixing 60 wt% of neutral protease and 40 wt% of papain.
The repair synergist is lycium barbarum polysaccharide.
The preparation method of the safe dental implant restoration liquid comprises the following steps: mixing the traditional Chinese medicine extract, the pearl extract, the repair synergist and the honey, and stirring at 300 revolutions per minute for 40 minutes to obtain the safe dental implant repair liquid.
Example 3
The safe dental implant restoration liquid comprises the following raw materials in parts by weight: 70 parts of medical stone water, 25 parts of traditional Chinese medicine extracting solution, 15 parts of pearl extracting solution, 2 parts of repair synergist and 8 parts of honey.
The preparation method of the traditional Chinese medicine extracting solution comprises the following steps:
(1) respectively drying 25 parts of honeysuckle, 25 parts of gallnut, 8 parts of lithospermum, 8 parts of scutellaria baicalensis and 3 parts of angelica dahurica at 70 ℃ for 8 hours, crushing, sieving with a 80-mesh sieve, and uniformly mixing to obtain traditional Chinese medicine powder;
(2) adding water 10 times the weight of the Chinese medicinal powder into the Chinese medicinal powder, keeping the temperature at 95 deg.C for 55 min, filtering with 300 mesh filter cloth, and collecting filtrate A; adding water 8 times the weight of the Chinese medicinal powder into the residue, keeping the temperature at 95 deg.C for 45 min, filtering with 300 mesh filter cloth to remove residue to obtain filtrate B, and mixing the two filtrates to obtain Chinese medicinal extractive solution.
The preparation method of the medical stone water comprises the following steps:
(1) cleaning the surface of medical stone, airing the surface moisture, crushing, and sieving by a 100-mesh sieve to obtain medical stone powder;
(2) mixing medical stone powder and water according to a solid-to-liquid ratio of 1 g: mixing 15mL of the components, standing the mixture for 18 hours at 25 ℃, adjusting the pH value to 6.0 by using acid liquor, and stirring the mixture for 4 hours at 70 ℃ at 200 rpm to obtain medical stone mixed solution;
(3) centrifuging the Maifanitum mixed solution at 6000 rpm for 15 min, collecting supernatant, and filtering with membrane with pore diameter of 0.22 μm to obtain Maifanitum water.
The preparation method of the pearl extract comprises the following steps:
(1) cleaning Margarita, removing impurities, pulverizing, and sieving with 80 mesh sieve to obtain coarse Margarita powder;
(2) adding zirconia ball with diameter of 0.1mm into the grinding cylinder of sanding dispersion machine, adding ethanol into the feeding mixing cylinder, starting the grinding machine, rotating speed of the main machine is 1800 rpm, feeding pressure is 0.35kPa, adding crude pearl powder simultaneously, and the solid-to-liquid ratio of the crude pearl powder and the ethanol is 1 g: 20mL, the mass ratio of the crude pearl powder to the zirconia balls is 1: 30, grinding for 70 minutes to obtain pearl powder slurry, centrifuging the pearl powder slurry at 4000 rpm/separation for 15 minutes, and drying the solid at 65 ℃ for 7 hours to obtain nano-scale pearl powder;
(3) nano-scale pearl powder and water are mixed according to the solid-to-liquid ratio of 1 g: mixing 30mL of the mixed solution, adding mixed enzyme, wherein the adding amount of the mixed enzyme is 0.3 percent of the mass of the nano-scale pearl powder, stirring for 4 hours at the temperature of 55 ℃ at 200 rpm, and preserving the heat for 4 minutes at the temperature of 98 ℃ to obtain enzymatic hydrolysate;
(4) centrifuging the enzymolysis solution at 4000 rpm for 15 min, collecting supernatant, and filtering with membrane with pore diameter of 0.22 μm to obtain Margarita extractive solution.
The mixed enzyme is prepared by mixing 60 wt% of neutral protease and 40 wt% of papain.
The acid solution is an organic acid aqueous solution with the mass fraction of 8%.
The organic acid is citric acid.
The repair synergist is lycium barbarum polysaccharide.
The preparation method of the safe dental implant restoration liquid comprises the following steps: adding the traditional Chinese medicine extract, the pearl extract, the repair synergist and the honey into the medical stone water, and stirring at 300 revolutions per minute for 40 minutes to obtain the safe dental implant repair liquid.
Example 4
Essentially the same as example 3, except that the organic acid was tartaric acid.
Example 5
Essentially the same as example 3 except that the organic acid was lactic acid.
Example 6
The safe dental implant restoration liquid comprises the following raw materials in parts by weight: 70 parts of medical stone water, 25 parts of traditional Chinese medicine extracting solution, 15 parts of pearl extracting solution, 2 parts of repair synergist and 8 parts of honey.
The preparation method of the traditional Chinese medicine extracting solution comprises the following steps:
(1) respectively drying 25 parts of honeysuckle, 25 parts of gallnut, 8 parts of lithospermum, 8 parts of scutellaria baicalensis and 3 parts of angelica dahurica at 70 ℃ for 8 hours, crushing, sieving with a 80-mesh sieve, and uniformly mixing to obtain traditional Chinese medicine powder;
(2) adding water 10 times the weight of the Chinese medicinal powder into the Chinese medicinal powder, keeping the temperature at 95 deg.C for 55 min, filtering with 300 mesh filter cloth, and collecting filtrate A; adding water 8 times the weight of the Chinese medicinal powder into the residue, keeping the temperature at 95 deg.C for 45 min, filtering with 300 mesh filter cloth to remove residue to obtain filtrate B, and mixing the two filtrates to obtain Chinese medicinal extractive solution.
The preparation method of the medical stone water comprises the following steps:
(1) cleaning the surface of medical stone, airing the surface moisture, crushing, and sieving by a 100-mesh sieve to obtain medical stone powder;
(2) mixing medical stone powder and water according to a solid-to-liquid ratio of 1 g: mixing 15mL of the components, standing the mixture for 18 hours at 25 ℃, adjusting the pH value to 6.0 by using acid liquor, and stirring the mixture for 4 hours at 70 ℃ at 200 rpm to obtain medical stone mixed solution;
(3) centrifuging the Maifanitum mixed solution at 6000 rpm for 15 min, collecting supernatant, and filtering with membrane with pore diameter of 0.22 μm to obtain Maifanitum water.
The preparation method of the pearl extract comprises the following steps:
(1) cleaning Margarita, removing impurities, pulverizing, and sieving with 80 mesh sieve to obtain coarse Margarita powder;
(2) adding zirconia ball with diameter of 0.1mm into the grinding cylinder of sanding dispersion machine, adding ethanol into the feeding mixing cylinder, starting the grinding machine, rotating speed of the main machine is 1800 rpm, feeding pressure is 0.35kPa, adding crude pearl powder simultaneously, and the solid-to-liquid ratio of the crude pearl powder and the ethanol is 1 g: 20mL, the mass ratio of the crude pearl powder to the zirconia balls is 1: 30, grinding for 70 minutes to obtain pearl powder slurry, centrifuging the pearl powder slurry at 4000 rpm/separation for 15 minutes, and drying the solid at 65 ℃ for 7 hours to obtain nano-scale pearl powder;
(3) nano-scale pearl powder and water are mixed according to the solid-to-liquid ratio of 1 g: mixing 30mL of the mixed solution, adding mixed enzyme, wherein the adding amount of the mixed enzyme is 0.3 percent of the mass of the nano-scale pearl powder, stirring for 4 hours at the temperature of 55 ℃ at 200 rpm, and preserving the heat for 4 minutes at the temperature of 98 ℃ to obtain enzymatic hydrolysate;
(4) centrifuging the enzymolysis solution at 4000 rpm for 15 min, collecting supernatant, and filtering with membrane with pore diameter of 0.22 μm to obtain Margarita extractive solution.
The mixed enzyme is prepared by mixing 60 wt% of neutral protease and 40 wt% of papain.
The acid solution is an organic acid aqueous solution with the mass fraction of 8%.
The organic acid is lactic acid.
The repair synergist is soybean polysaccharide.
The preparation method of the safe dental implant restoration liquid comprises the following steps: adding the traditional Chinese medicine extract, the pearl extract, the repair synergist and the honey into the medical stone water, and stirring at 300 revolutions per minute for 40 minutes to obtain the safe dental implant repair liquid.
The anti-inflammatory effect test result of the obtained safe dental implant restoration liquid is as follows: the swelling inhibition rate is 39.87%; the result of the bacteriostatic effect test is as follows: the bacteriostatic rate of Candida albicans is 88.42%, and the bacteriostatic rate of Staphylococcus aureus is 89.06%.
Example 7
The safe dental implant restoration liquid comprises the following raw materials in parts by weight: 70 parts of medical stone water, 25 parts of traditional Chinese medicine extracting solution, 15 parts of pearl extracting solution, 2 parts of repair synergist and 8 parts of honey.
The preparation method of the traditional Chinese medicine extracting solution comprises the following steps:
(1) respectively drying 25 parts of honeysuckle, 25 parts of gallnut, 8 parts of lithospermum, 8 parts of scutellaria baicalensis and 3 parts of angelica dahurica at 70 ℃ for 8 hours, crushing, sieving with a 80-mesh sieve, and uniformly mixing to obtain traditional Chinese medicine powder;
(2) adding water 10 times the weight of the Chinese medicinal powder into the Chinese medicinal powder, keeping the temperature at 95 deg.C for 55 min, filtering with 300 mesh filter cloth, and collecting filtrate A; adding water 8 times the weight of the Chinese medicinal powder into the residue, keeping the temperature at 95 deg.C for 45 min, filtering with 300 mesh filter cloth to remove residue to obtain filtrate B, and mixing the two filtrates to obtain Chinese medicinal extractive solution.
The preparation method of the medical stone water comprises the following steps:
(1) cleaning the surface of medical stone, airing the surface moisture, crushing, and sieving by a 100-mesh sieve to obtain medical stone powder;
(2) mixing medical stone powder and water according to a solid-to-liquid ratio of 1 g: mixing 15mL of the components, standing the mixture for 18 hours at 25 ℃, adjusting the pH value to 6.0 by using acid liquor, and stirring the mixture for 4 hours at 70 ℃ at 200 rpm to obtain medical stone mixed solution;
(3) centrifuging the Maifanitum mixed solution at 6000 rpm for 15 min, collecting supernatant, and filtering with membrane with pore diameter of 0.22 μm to obtain Maifanitum water.
The preparation method of the pearl extract comprises the following steps:
(1) cleaning Margarita, removing impurities, pulverizing, and sieving with 80 mesh sieve to obtain coarse Margarita powder;
(2) adding zirconia ball with diameter of 0.1mm into the grinding cylinder of sanding dispersion machine, adding ethanol into the feeding mixing cylinder, starting the grinding machine, rotating speed of the main machine is 1800 rpm, feeding pressure is 0.35kPa, adding crude pearl powder simultaneously, and the solid-to-liquid ratio of the crude pearl powder and the ethanol is 1 g: 20mL, the mass ratio of the crude pearl powder to the zirconia balls is 1: 30, grinding for 70 minutes to obtain pearl powder slurry, centrifuging the pearl powder slurry at 4000 rpm/separation for 15 minutes, and drying the solid at 65 ℃ for 7 hours to obtain nano-scale pearl powder;
(3) nano-scale pearl powder and water are mixed according to the solid-to-liquid ratio of 1 g: mixing 30mL of the mixed solution, adding mixed enzyme, wherein the adding amount of the mixed enzyme is 0.3 percent of the mass of the nano-scale pearl powder, stirring for 4 hours at the temperature of 55 ℃ at 200 rpm, and preserving the heat for 4 minutes at the temperature of 98 ℃ to obtain enzymatic hydrolysate;
(4) centrifuging the enzymolysis solution at 4000 rpm for 15 min, collecting supernatant, and filtering with membrane with pore diameter of 0.22 μm to obtain Margarita extractive solution.
The mixed enzyme is prepared by mixing 60 wt% of neutral protease and 40 wt% of papain.
The acid solution is an organic acid aqueous solution with the mass fraction of 8%.
The organic acid is lactic acid.
The repairing synergist is a mixture of lycium barbarum polysaccharide and soybean polysaccharide, wherein the weight ratio of the lycium barbarum polysaccharide to the soybean polysaccharide is (4): 1.
the preparation method of the safe dental implant restoration liquid comprises the following steps: adding the traditional Chinese medicine extract, the pearl extract, the repair synergist and the honey into the medical stone water, and stirring at 300 revolutions per minute for 40 minutes to obtain the safe dental implant repair liquid.
The anti-inflammatory effect test result of the obtained safe dental implant restoration liquid is as follows: the swelling inhibition rate is 50.46%; the result of the bacteriostatic effect test is as follows: the bacteriostasis rate of the candida albicans is 93.58 percent, and the bacteriostasis rate of the staphylococcus aureus is 94.67 percent.
Test example 1
The anti-inflammatory effects of the safe dental implant restoration solutions prepared in examples 1-5 and comparative examples 1-2 were tested, and the specific test results are shown in table 1.
Table 1 table of anti-inflammatory effect test results of safe dental implant restoration solution
Test example 2
The antibacterial effect of the safe dental implant restoration liquid prepared in the examples 1-5 and the comparative examples 1-2 is tested, and the specific test results are shown in table 2.
Table 2 bacteriostatic effect test result table
| Candida albicans inhibitory rate (%) | Staphylococcus aureus inhibitory rate (%) | |
| Example 1 | 80.18 | 82.52 |
| Example 2 | 85.67 | 86.85 |
| Comparative example 1 | 81.24 | 82.89 |
| Comparative example 2 | 82.89 | 83.25 |
| Example 3 | 87.56 | 88.72 |
| Example 4 | 86.78 | 87.36 |
| Example 5 | 89.34 | 90.61 |
Example 2 the anti-inflammatory and bacteriostatic effects of the obtained safe dental implant restoration solution are better than those of example 1 by adopting medical stone water. The medical stone water contains elements beneficial to human bodies and elements essential to the human bodies, and can promote metabolism, improve immunity and further improve the effects of resisting inflammation and inhibiting bacteria.
In the example 2, the medical stone water and the pearl extract are adopted simultaneously, so that the anti-inflammatory and antibacterial effects of the obtained safe dental implant restoration liquid are superior to those of the safe dental implant restoration liquid in the comparative examples 1-2. The pearl extract contains various free amino acids and microelements, which interact with beneficial elements in Maifanitum water, are beneficial for organism absorption, and improve antiinflammatory and antibacterial effects.
Examples 3-5 preparation of medical stone water in a weak acid environment, the anti-inflammatory and bacteriostatic effects of the obtained safe dental implant restoration solution were superior to those of example 2. This is because some of the beneficial elements in Maifanitum exist in the form of oxides, which is favorable for dissolution of these beneficial elements in an acidic environment. Meanwhile, the lactic acid is adopted to adjust the pH in the embodiment 5, and the effect is better.
Claims (4)
1. The safe dental implant restoration liquid is characterized by comprising the following raw materials in parts by weight: 60-80 parts of medical stone water, 20-30 parts of traditional Chinese medicine extract, 10-20 parts of pearl extract, 1-5 parts of repair synergist and 5-10 parts of honey;
the preparation method of the medical stone water comprises the following steps:
(1) cleaning the surface of medical stone, airing the surface moisture, crushing, and sieving by a 100-mesh sieve to obtain medical stone powder;
(2) mixing medical stone powder and water according to a solid-to-liquid ratio of 1 g: (10-20) mL, standing at 20-30 ℃ for 12-24 hours, adjusting the pH to 5.5-6.5 with acid liquor, and stirring at 60-80 ℃ for 2-6 hours at 300 rpm under 100 ℃ to obtain medical stone mixed solution;
(3) centrifuging the Maifanitum mixed solution at 4000-;
the preparation method of the traditional Chinese medicine extracting solution comprises the following steps:
(1) drying flos Lonicerae 20-30 parts, Galla chinensis 20-30 parts, radix Arnebiae 5-10 parts, Scutellariae radix 5-10 parts, and radix Angelicae Dahuricae 1-5 parts at 60-80 deg.C for 6-10 hr respectively, pulverizing, and mixing to obtain Chinese medicinal powder;
(2) adding water 8-12 times the weight of the Chinese medicinal powder into the Chinese medicinal powder, maintaining the temperature at 90-98 deg.C for 50-60 min, filtering with 300 mesh filter cloth, and collecting filtrate A; adding water 5-10 times the weight of the Chinese medicinal powder into the residue, keeping the temperature at 90-98 deg.C for 40-50 min, filtering the residue with 300 mesh filter cloth to obtain filtrate B, and mixing the two filtrates to obtain Chinese medicinal extractive solution;
the preparation method of the pearl extract comprises the following steps:
(1) cleaning Margarita, removing impurities, and pulverizing to obtain coarse Margarita powder;
(2) adding zirconia balls with the diameter of 0.1mm into a grinding cylinder of a sanding dispersion machine, adding ethanol into a feeding stirring cylinder, starting the grinding machine, adding coarse pearl powder simultaneously, wherein the solid-to-liquid ratio of the coarse pearl powder to the ethanol is 1 g: (15-25) mL, grinding for 65-75 minutes to obtain pearl powder slurry, centrifuging the pearl powder slurry for 10-20 minutes at 3000-5000 rpm/min, and drying the solid at 60-70 ℃ for 6-8 hours to obtain nano-scale pearl powder;
(3) nano-scale pearl powder and water are mixed according to the solid-to-liquid ratio of 1 g: (20-40) mL, adding mixed enzyme, wherein the addition amount of the mixed enzyme is 0.1-0.5% of the mass of the nano-scale pearl powder, stirring at 50-60 ℃ for 2-6 hours at 300 revolutions per minute, and preserving the temperature at 95-100 ℃ for 3-5 minutes to obtain enzymatic hydrolysate;
(4) centrifuging the enzymolysis solution at 3000-;
the mixed enzyme is formed by mixing 50-70 wt% of neutral protease and 30-50 wt% of papain;
the repair synergist is a mixture of lycium barbarum polysaccharide and soybean polysaccharide, wherein the lycium barbarum polysaccharide and the soybean polysaccharide are in a mass ratio of (2-6): 1.
2. the safe dental implant restoration solution according to claim 1, wherein the acid solution is an aqueous solution of 5-10% by mass of an organic acid, and the organic acid is one of citric acid, tartaric acid and lactic acid.
3. The safe dental implant restorative liquid of claim 2, wherein the organic acid is lactic acid.
4. The preparation method of the safe dental implant restoration solution of any one of claims 1 to 3, comprising the following steps: adding the traditional Chinese medicine extract, the pearl extract, the repair synergist and the honey into the medical stone water, and stirring for 30-60 minutes at 200-400 rpm to obtain the safe dental implant repair liquid.
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| CN101077079A (en) * | 2006-05-25 | 2007-11-28 | 西安格润森生物制药股份有限公司 | Plant-type antibacterial agent |
| CN104399973A (en) * | 2014-12-11 | 2015-03-11 | 北海银源医药科技有限公司 | Disperse protective agent prepared from pearl and momordica grosvenori extract and application thereof |
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