CN107875059B - Composition with acne removing effect - Google Patents
Composition with acne removing effect Download PDFInfo
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- CN107875059B CN107875059B CN201711243357.2A CN201711243357A CN107875059B CN 107875059 B CN107875059 B CN 107875059B CN 201711243357 A CN201711243357 A CN 201711243357A CN 107875059 B CN107875059 B CN 107875059B
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/96—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution
- A61K8/97—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution from algae, fungi, lichens or plants; from derivatives thereof
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/19—Cosmetics or similar toiletry preparations characterised by the composition containing inorganic ingredients
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/30—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds
- A61K8/64—Proteins; Peptides; Derivatives or degradation products thereof
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
- A61Q19/00—Preparations for care of the skin
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2800/00—Properties of cosmetic compositions or active ingredients thereof or formulation aids used therein and process related aspects
- A61K2800/80—Process related aspects concerning the preparation of the cosmetic composition or the storage or application thereof
- A61K2800/805—Corresponding aspects not provided for by any of codes A61K2800/81 - A61K2800/95
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2800/00—Properties of cosmetic compositions or active ingredients thereof or formulation aids used therein and process related aspects
- A61K2800/80—Process related aspects concerning the preparation of the cosmetic composition or the storage or application thereof
- A61K2800/82—Preparation or application process involves sonication or ultrasonication
Abstract
The invention relates to a composition with an acne removing effect, which is characterized by comprising the following components in parts by weight: 10-15 parts of traditional Chinese medicine extract, 3-5 parts of ficus pumila cryptocephala extract, 3-5 parts of momordica grosvenori extract, 4-6 parts of lonicera edulis extract, 2-4 parts of cantonese rubus suavissima fruit extract, 6-8 parts of mango extract, 6-8 parts of longan extract, 10-12 parts of apple extract, 0.5 part of nano graphene, 0.01 part of fibroblast growth factor, 1-2 parts of dextran, 0.05-0.1 part of polyglutamic acid, 2-4 parts of mannitol and 65-85 parts of deionized water. The nano graphene, the fibroblast growth factor and active ingredients in the all-pure natural fruit extract are combined for the first time, and the preparation method does not contain a preservative, has no stimulation, continuously and efficiently resists inflammation and bacteria, removes acne and prevents pockmark formation. Because the biological transdermal enhancer is derived from the whole fruit extract, the biological transdermal enhancer has good biocompatibility, does not damage the skin and has safety.
Description
Technical Field
The invention belongs to the technical field of preparation of daily skin care products, and particularly relates to a composition with an acne removing effect.
Background
Acne is also known as acne, and the occurrence of acne is closely related to factors such as excessive sebum secretion, blockage of pilosebaceous ducts, bacterial infection and inflammatory reaction. After adolescence, the level of androgen, particularly Cudrazone in human body is rapidly increased, so as to promote the development of sebaceous glands and produce a large amount of sebum. Meanwhile, abnormal keratinization of the pilosebaceous canal causes the canal to be blocked, sebum is obstructed to be discharged, and a keratoplug, namely micro acne, is formed. A number of microorganisms in the hair follicle, particularly propionibacterium acnes, multiply in number, and lipases produced by propionibacterium acnes break down sebum to produce free fatty acids, while chemotactic inflammatory cells and mediators, ultimately inducing and exacerbating the inflammatory response.
With the improvement of the consumption consciousness of people, consumers pay more and more attention to the safety of the product when selecting the cleaning product and the skin care product, and the safety of the product is far more important than the curative effect and the cosmetic effect. The natural plant and animal extracts have low sensitization rate and high safety, and products prepared by the active ingredient extracts are more and more valued and favored by consumers.
The nano graphene can kill more than 10 bacteria such as pseudomonas aeruginosa, escherichia coli and the like, because the nano graphene is a series of carbon atoms, like a layer of very thin and very dense cloth, and three ways of dealing with the bacteria are provided: the first is direct cutting, because the graphene is nano-scale and the bacteria are micro-scale, the former is 1000 times smaller than the latter, and the graphene is like a sharp knife to directly cut the bacteria; the second is starvation bacteria, and the nano-scale graphene can tightly wrap the bacteria like cloth, so that the bacteria can not absorb nutrition and can be alive and starved; the third one is slow elimination, because the graphene is too small, the graphene can be swallowed by bacteria, and the bacteria can be slowly eliminated just like people eating foreign matters can pull the belly uncomfortable, and the bacteria can not generate drug resistance, and the physical sterilization method of the graphene has good acne removing and inflammation diminishing effects on the skin.
At present, hormone, antibiotics, tretinoin and other components are used in a plurality of acne-removing products, so that the acne-removing products have high irritation, easily cause drug resistance of skin to bacteria, and are continuously questioned in safety. And such products generally do not have long lasting bacteriostatic and anti-acne effects, nor the ability to remove acne marks and stains.
Disclosure of Invention
In order to overcome the defects of the prior art, the invention aims to provide a composition with an acne removing effect and a preparation method of freeze-dried powder thereof, which can effectively solve the problems that the extraction efficiency of the currently produced product on plant fruit active substances exerting the drug effect is low, the skin is damaged by preservatives and hormones, the skin generates drug resistance, the oxidation resistance is weak, and the formation of pockmarks and acne scars cannot be effectively prevented.
The invention is realized by the following technical scheme:
the composition with the acne removing effect is characterized by comprising the following components in parts by weight: 10-15 parts of traditional Chinese medicine extract, 3-5 parts of ficus pumila cryptocephala extract, 3-5 parts of momordica grosvenori extract, 4-6 parts of lonicera edulis extract, 2-4 parts of cantonese rubus suavissima fruit extract, 6-8 parts of mango extract, 6-8 parts of longan extract, 10-12 parts of apple extract, 0.5 part of nano graphene, 0.01 part of fibroblast growth factor, 1-2 parts of dextran, 0.05-0.1 part of polyglutamic acid, 2-4 parts of mannitol and 65-85 parts of deionized water; the traditional Chinese medicine extract comprises the following components in parts by weight: 5 parts of licorice extract and 5 parts of salvia miltiorrhiza extract;
the preparation method of the longan extract adopts a pectinase and cellulase double-enzyme simultaneous enzymolysis extraction method, and comprises the following specific steps: drying fresh longan with hot air at 80 deg.C, peeling, removing core to obtain dried arillus longan, sealing, and storing at 4 deg.C; weighing dried longan pulp, adding deionized water, soaking for 12h with a material-water ratio of 1:3, and mashing with a high-speed tissue mashing machine; adding deionized water into the smashed material, wherein the material-water ratio is 1:12, performing enzymolysis by using two enzymes of pectinase and cellulase at the same time, wherein the pectinase is 0.6 percent and the cellulase is 0.05 percent, the pH value is adjusted to be 3.1, the enzymolysis temperature is 52 ℃, the enzymolysis time is 160min, after the enzymolysis is finished, the enzyme is inactivated for 30min at the temperature of 90 ℃, filtering by a 200-mesh sieve to collect filtrate, concentrating the filtrate into semi-flowing pulp, and then mixing the pulp with the ratio of 1: dissolving the mixture in deionized water at a volume ratio of 10, stirring and shaking up to obtain the longan extract.
Further, the preparation method of the Guangxi rubus suavissimus fruit extract comprises the following steps: weighing Guangxi sweet tea peel and pulp, placing in a mortar, adding 85% methanol solution, grinding, completely transferring to a container with a plug, ultrasonically extracting for 50 min at 40 deg.C and 200W ultrasonic power, cooling at room temperature, and centrifuging the extractive solution at 4500 rpm/min for 10 min; after concentrating the supernatant from the centrifugation to a semi-fluid slurry, the slurry was mixed at a ratio of 1: dissolving the extract in deionized water at a volume ratio of 10, stirring and shaking uniformly to obtain the Guangxi rubus suavissimus fruit extract.
Further, the preparation methods of the cryptocarpus ficus pumila extract, the momordica grosvenori extract and the apple extract comprise the following steps: respectively adding 90% ethanol solution into peel and pulp of various raw materials pulverized by liquid nitrogen at a solid-liquid ratio of 1:33, and ultrasonically extracting at 31 deg.C and 200W for 38min to obtain extractive solution; filtering the extractive solution, and concentrating under reduced pressure to obtain slurry; the slurry was mixed at a ratio of 1: dissolving 10 volume ratio in deionized water, stirring, and shaking to obtain Ficus pumila Linn hidden fruit extract, fructus Siraitiae Grosvenorii extract and fructus Mali Pumilae extract.
Further, the preparation method of the lonicera edulis extract comprises the following steps: washing away silt on the surface of the lonicera edulis by flowing water, introducing an ozone generator into sterile water to wash the lonicera edulis again, then draining and removing the core, crushing pulp into pulp, adding deionized water according to 40% of the volume of the pulp, simultaneously adding 3.5% of pectinase, stirring uniformly, and standing for 30min at 35 ℃; adding the supernatant into 95% ethanol solution, ultrasonic extracting at 40 deg.C and 200W ultrasonic power for 15min, and centrifuging at 4000 rpm/min for 10 min; after concentrating the supernatant from the centrifugation to a semi-fluid slurry, the slurry was mixed at a ratio of 1: dissolving the mixture in deionized water according to the volume ratio of 10, stirring and shaking up to obtain the lonicera edulis extract.
Further, the preparation method of the mango extract comprises the following specific steps: weighing mango pulp and peel freeze-dried powder, adding 80% acetone at a solid-to-liquid ratio of 1:20, ultrasonically extracting for 30min at an ultrasonic frequency of 60kHz and a power of 30W, centrifuging for 10min at 7000rpm/min, taking supernatant, repeating the above operations twice, and combining the supernatants; and (3) evaporating the combined supernatant on a rotary evaporator to dryness, and mixing the product obtained after evaporation to dryness in a ratio of 1: dissolving the mixture in deionized water at a volume ratio of 10, stirring and shaking up to obtain the mango extract.
A preparation method of freeze-dried powder of a composition with an acne removing effect is characterized by comprising the following steps:
the method comprises the following steps: putting polyglutamic acid and deionized water into a homogenizer according to a proportion, homogenizing for 10min at 78 ℃, completely dissolving to obtain A, and then placing A for natural cooling;
step two: when the temperature of A is reduced to about 50 ℃, mannitol and dextran are added according to the proportion and are uniformly mixed to obtain B;
step three: when the temperature of the B is reduced to about 30 ℃, adding the traditional Chinese medicine extract, the ficus pumila cryptocephala extract, the momordica grosvenori extract, the lonicera edulis extract, the Guangxi rubus suavissimus fruit extract, the mango extract, the longan extract, the apple extract, the nano-graphene and the fibroblast growth factor in proportion, uniformly stirring to obtain C, then freezing the C at-25 ℃ for 20 hours, and discharging after the plate alignment is qualified;
step four: filling the frozen C, placing the C in a vacuum freeze drying device, and carrying out vacuum freeze drying treatment for 35h at the temperature of-50 ℃ and the vacuum degree of 7Pa to obtain the freeze-dried powder containing the composition with the acne removing effect.
The Ficus pumila is a evergreen vine with edible, medicinal and ecological values. The fig fruit is rich in nutrition, and the parts of the fig, the perianth and the like contain rich nutritional ingredients. The tall fig fruit contains high content of fatty acid which is up to 30.13 percent, mainly unsaturated fatty acid such as alpha-linolenic acid, linoleic acid and the like, and also contains rich crude fiber (33.4 percent), protein (15.7 percent), pectin (15.15 percent) and the like, and the analysis of amino acids shows that the tall fig fruit contains 17 amino acids, wherein the content of glutamic acid is the highest, 8 amino acids which are necessary for a human body are complete, and the contents of various mineral elements and vitamins are also rich. Modern researches show that the cryptocarpus ficus pumila linn fruit contains various compounds such as flavonoids, triterpenes, coumarins and glycosides, has excellent antioxidant activity and antibacterial activity, and has the effects of dispelling wind and removing dampness, promoting blood circulation and removing meridian obstruction, relieving swelling and detoxifying and resisting inflammation, more importantly, the cryptocarpus pumila linn fruit contains alexandrine, the component is also a natural stabilizer, and the natural anti-inflammatory, antibacterial and antioxidant raw material of the cryptocarpus pumila linn fruit can be simultaneously utilized as the natural stabilizer without adding an industrial stabilizer in a raw material system.
Momordica grosvenori is the fruit of plants of genus Momordica of family Cucurbitaceae of class Dicotyledoneae, and is named as "Momordica grosvenori" in the plant science. The fructus Siraitiae Grosvenorii extract and its components separated from fructus Siraitiae Grosvenorii have effects of clearing away heat and toxic materials, relieving cough, clearing lung-heat, inhibiting bacteria, relieving inflammation, and enhancing immunity. The main active component is mogrosides which are cucurbitacin triterpenoid sweet glycoside compounds. The fructus Siraitiae Grosvenorii polysaccharide and mogroside have antibacterial, antiviral, and radioprotective effects. The momordica grosvenori also contains flavonoid glycoside and protein amino acids, contains 17 amino acid components and 16 inorganic trace elements, has the VC content which is much higher than that of the kiwi fruit, and is a natural medicine fruit with rich nutrition.
The indigo honeysuckle belongs to Caprifoliaceae, is a wild berry plant, has extremely small seeds, extremely high juice yield, contains abundant amino acids, vitamins and bioactive substances, has excellent antioxidant activity and extremely high nutritional and health-care values. Anthocyanin and flavonoid compounds are used as functional components in the lonicera edulis fruit and have a plurality of beneficial physiological functions and pharmacological actions, wherein the anthocyanin has the functions of eliminating free radicals, resisting aging, resisting oxidation, resisting inflammation and improving immunity, the flavonoid compounds can promote blood circulation in human bodies, and the combined action of amino acid, vitamin, anthocyanin and flavonoid substances can effectively resist inflammation, promote wound healing and prevent scar formation. Amino acids, vitamins and anthocyanins in the lonicera edulis can also utilize effective flavonoid components in other plant and fruit extracts of the invention to achieve the effects of resisting inflammation, resisting oxidation, promoting wound healing and preventing scar formation to achieve synergistic effect.
Guangxi sweet tea is a new variety of plants in the genus Rubus of the family Rosaceae, and is a specific plant in Guangxi. The sweet tea fruit has rich and complete nutrition, soft and juicy fruit, pleasant color and luster, and can be processed into jam, fruit juice, fruit wine and other products due to sweet and rich fragrance and nutrition. The fruit medicine can improve eyesight, tonify kidney, calm nerves and regulate qi. Recent researches show that the rubus suavissimus fruit extract contains abundant terpenoids and volatile oil components, wherein the main components are quercetin and kaempferol, the two components have good antibacterial and anti-inflammatory effects and have good treatment effects on acne and pox skin, and meanwhile, the rubus suavissimus fruit extract is also a natural preservative, and no preservative is required to be additionally added in the raw materials.
Mango is an original Indian evergreen arbor, leafy leatheroid and intergrowth; warm in nature, small in flower, yellow or light red, forming a terminal panicle. Mango fruits contain sugar, protein, crude fiber, and the precursor carotene component of vitamin a contained in mango is particularly high and is rare in all fruits. Secondly, the content of vitamin C is not low. Minerals, proteins, fats, carbohydrates, etc., are also the main nutritional components. Mango is one of the famous tropical fruits, and is well liked by people because of fine and smooth pulp and unique flavor, so that mango is called as the "tropical fruit king". The most unique and important component in the mango fruit extract is mangiferin, which is an important polyphenol compound in mango and belongs to xanthone compounds.
Longan is a famous subtropical fruit in China, the fruit is popular name longan, and is advocated as a "holy fruit". Longan has high nutritive value and is one of important medicinal materials. The longan pulp contains rich phospholipid components: 3.95 plus or minus 0.12 of total phospholipid (mg/g), 13.8 of lysophospholipid phthalein choline, 49.5 of phosphatidic acid choline, 2.4 of phosphatide inositol, 3.8 of phosphatide serine, 3.8 of phosphatide ethanolamine, 2.8 of phosphatidic acid and 19.7 of phosphatide glycerol, wherein the components have strong effects on resisting skin aging, improving the immune function of the organism and the like. Meanwhile, the longan pulp also contains rich components of polysaccharide, polyphenol alcohol and terpenoid, wherein the most important component is ginsenoside, and the components have the functions of high-efficiency anti-inflammation, detoxification and cell growth promotion. Can be cooperated with other antibacterial components in the invention, so that the antibacterial and anti-inflammatory effects of the product of the invention are greatly improved.
The apple is one of ten fruits in the world, the apple has high health care value, and the apple phenolic substances are important substances influencing the health care effect of the apple. Phenolics are one of the important secondary metabolites found in nature and are an important source of natural antioxidants. The apple fruit is rich in sugar, protein, organic acid, vitamins and various phenolic components, wherein anthocyanin, phenolic acid and flavonoid substances are main phenolic components in the apple. According to the nutritionists survey in the netherlands, the united states, etc., apple is the third largest dietary source of phenolics behind tea and onion. Many pharmacological studies show that the phenolic substances in the apples have various biological activities, can remove excessive oxygen free radicals in the body and have strong antioxidant effect. The apple extract can effectively reduce capillary permeability, improve microcirculation on the skin surface, and promote wound healing and scar fading. Apple extract itself is also a preservative.
The glycyrrhizin is called as whitening queen, and the extract of the glycyrrhizin can resist ultraviolet rays, inhibit the activity of tyrosinase, and improve the problems of rough skin, water deficiency, inflammation and the like. The effective components with whitening effect are flavonoids such as liquiritin, liquiritigenin, glycyrrhetinic acid, glycyrrhizin and some polysaccharides. Research results show that when the liquorice is used alone, the inhibition rate of the liquorice on the tyrosinase activity is very low, and the inhibition rate of the compound on the tyrosinase activity is increased by 5-6 times.
The Saviae Miltiorrhizae radix chemical components mainly comprise fat-soluble diterpene compounds such as tanshinone, water-soluble phenolic acids such as tanshinol, salvianolic acid and polysaccharides. When the licorice extract and the salvia extract are used independently, the free radical scavenging oxidation resistance is weak, and the light stability is poor, but the licorice extract and the salvia extract have better light stability and more multiplied oxidation resistance when being compounded. The compound extract can have important influence on the proliferation of human epidermal fibroblasts and human epidermal keratinocytes, and the compound extract can promote the proliferation of the human epidermal fibroblasts, promote metabolism, inhibit the proliferation of the human epidermal keratinocytes, soften the stratum corneum and promote wound healing without stimulating the skin. The compound extract of the licorice extract and the salvia extract can well inhibit the activity of the tyrosinase. Namely, the combined use of the licorice extract, the salvia extract and the nano graphene can greatly improve the inhibition and synergy effects on tyrosinase activity and the anti-inflammatory activity.
The Chinese medicinal materials in the invention are matched and used as the medicaments, so that the effects of resisting bacteria, diminishing inflammation, removing acnes and promoting tissue regeneration can be achieved through mutual synergism. Meanwhile, the preservative can be cooperated with the Guangxi beet fruit extract and the apple extract to form a more complete and effective preservative system.
Fibroblast Growth Factors (FGFs) are multifunctional nonspecific active substances, and have the effects of promoting DNA synthesis and cell division of cells derived from various mesoderm and neuroectoderm such as endothelial cells and fibroblasts. FGFs also play a bidirectional regulating role in the synthesis of collagens of fibroblasts, continuously improve and repair the structure and strength of tissues through continuous synthesis, secretion, reconstruction and updating of the collagens, and can stimulate the expression of the collagens, thereby effectively inhibiting pathological scars generated by mass synthesis and excessive precipitation of the collagens and further improving the quality of wound healing.
The nano graphene can kill more than 10 bacteria such as pseudomonas aeruginosa, escherichia coli and the like, because the nano graphene is a series of carbon atoms, like a layer of very thin and very dense cloth, and three ways of dealing with the bacteria are provided: the first is direct cutting, because the graphene is nano-scale and the bacteria are micro-scale, the former is 1000 times smaller than the latter, and the graphene is like a sharp knife, so that the bacteria are directly cut off; the second is starvation bacteria, and the nano-scale graphene can tightly wrap the bacteria like cloth, so that the bacteria can not absorb nutrition and can be alive and starved; the third one is slow elimination, because the graphene is too small, the graphene can be swallowed by the bacteria, and the bacteria can be slowly eliminated just like people eating foreign matters can pull the belly uncomfortable, and the bacteria can not generate drug resistance, so that the physical sterilization method of the graphene has good acne removing and inflammation diminishing effects on the skin.
The fruit components with targeted effects, the nano graphene and the growth factors are used in a compounding manner, so that the problems caused by acne and comedo on the skin can be solved more efficiently, and meanwhile, the antibacterial, anti-inflammatory, antioxidant, healing promoting and repairing performances of the product are greatly improved.
The composition with the acne removing effect and the preparation method of the freeze-dried powder thereof have the following beneficial effects:
1. the composition with the acne removing effect and the preparation method of the freeze-dried powder thereof are combined with the nano-graphene, the fibroblast growth factor and active ingredients in the all-natural fruit extract for the first time, and the freeze-dried powder which does not contain a preservative and a stabilizer, has no stimulation, continuously and efficiently resists inflammation and bacteria, removes acne and prevents pockmarks from forming is prepared. The invention has no any industrial preservative, and the components of the invention have the advantages of safety, no toxicity, good water solubility, good thermal stability and wide action range of natural preservatives.
2. According to the composition with the acne removing effect and the preparation method of the freeze-dried powder of the composition, most of extracts are extracted by an ultrasonic extraction method, the extraction efficiency of the process is high, the utilization rate of active ingredients in fruit medicines is extremely high, and resources are not wasted. The invention adopts an ultrasonic extraction method to prepare the extract, utilizes special effects of strong vibration, higher acceleration, strong cavitation effect, stirring and the like generated by ultrasonic waves to destroy plant cells, enables a solvent to permeate into material cells, promotes chemical components in raw materials to be extracted to be dissolved in the solvent, and obtains the required chemical components through separation and purification. The extraction rate of the ultrasonic extraction for 30min is 9% higher than that of the conventional decocting method for 3 h.
3. The preparation method of the composition with the acne removing effect and the freeze-dried powder thereof adopts an enzymolysis extraction method, wherein a single kind of enzymolysis extraction method also has an enzymolysis extraction method of mixing a plurality of enzymes. The enzyme engineering technology is a biological engineering technology for extracting active ingredients of natural plants in recent years. In the extraction process of bioactive substances, the extraction conditions can be reduced by using enzymes, plant tissues can be decomposed under mild conditions, and the extraction efficiency is improved. The natural and effective preservative system is effectively formed by combining, no extra addition is needed, the skin-friendly effect is not stimulated, and the cost is also reduced.
4. The composition with the acne removing effect and the preparation method of the freeze-dried powder thereof and the multiple fruit extracts are a composite action system, and comprehensively utilize the effects of taking longan extracts as the first, Ficus pumila cravings fruit extracts and mango extracts as auxiliary synergistic anti-inflammatory components, the effects of antibacterial and antiviral components of fructus momordicae extracts and Guangxi beet fruit extracts, the effects of special light scar healing promoting components of indigo honeysuckle extracts and apple extracts, the effect of an antiseptic component of the common effect of the apple extracts and the Guangxi beet fruit extracts, and the Ficus pumila crassipes fruit extracts as a natural stabilizer. The components of the whole natural fruit extract can bring out the best in each other aiming at the problems of acne and pox. The invention combines the advantages of various raw materials, such as sufficient and perfect efficacy, and more efficiently plays the overall effects of continuous antibiosis, anti-inflammation, acne removal, scar lightening and cell proliferation promotion to regenerate the skin.
The composition with the acne removing effect and the preparation method of the freeze-dried powder thereof have good biocompatibility, do not damage skin and have safety because the biological transdermal enhancer is derived from plant fruit extracts.
Detailed Description
The invention discloses a composition with acne removing efficacy and a preparation method of freeze-dried powder thereof, and the invention is further explained by combining with a specific embodiment as follows:
example 1:
the composition with the acne removing effect comprises the following components in parts by weight:
10 parts of traditional Chinese medicine extract, 3 parts of ficus pumila cryptocarpus extract, 3 parts of momordica grosvenori extract, 4 parts of indigo honeysuckle extract, 2 parts of Guangxi rubus suavissimus fruit extract, 6 parts of mango extract, 6 parts of longan extract, 10 parts of apple extract, 0.5 part of nano graphene, 0.01 part of fibroblast growth factor, 1 part of dextran, 0.05 part of polyglutamic acid, 2 parts of mannitol and 65 parts of deionized water; the traditional Chinese medicine extract comprises the following components in parts by weight: 5 parts of licorice extract and 5 parts of salvia miltiorrhiza extract.
Example 2:
the composition with the acne removing effect comprises the following components in parts by weight:
13 parts of traditional Chinese medicine extract, 4 parts of ficus pumila cryptocephala extract, 4 parts of momordica grosvenori extract, 5 parts of indigo honeysuckle extract, 3 parts of Guangxi rubus suavissimus fruit extract, 7 parts of mango extract, 7 parts of longan extract, 11 parts of apple extract, 0.5 part of nano graphene, 0.01 part of fibroblast growth factor, 2 parts of dextran, 0.08 part of polyglutamic acid, 3 parts of mannitol and 70 parts of deionized water; the traditional Chinese medicine extract comprises the following components in parts by weight: 5 parts of licorice extract and 5 parts of salvia miltiorrhiza extract.
Example 3:
the composition with the acne removing effect comprises the following components in parts by weight:
15 parts of traditional Chinese medicine extract, 5 parts of ficus pumila cryptocephala extract, 5 parts of momordica grosvenori extract, 6 parts of lonicera edulis extract, 4 parts of cantonese rubus suavissima fruit extract, 8 parts of mango extract, 8 parts of longan extract, 12 parts of apple extract, 0.5 part of nano graphene, 0.01 part of fibroblast growth factor, 2 parts of dextran, 0.1 part of polyglutamic acid, 4 parts of mannitol and 85 parts of deionized water; the traditional Chinese medicine extract comprises the following components in parts by weight: 5 parts of licorice extract and 5 parts of salvia miltiorrhiza extract.
The raw material extraction method in each example is as follows:
the preparation method of the longan extract adopts a pectinase and cellulase double-enzyme simultaneous enzymolysis extraction method, and comprises the following specific steps: drying fresh longan with hot air at 80 deg.C, peeling, removing core to obtain dried arillus longan, sealing, and storing at 4 deg.C; weighing dried longan pulp, adding deionized water, soaking for 12h with a material-water ratio of 1:3, and mashing with a high-speed tissue mashing machine; adding deionized water into the smashed material, wherein the material-water ratio is 1:12, performing enzymolysis by using two enzymes of pectinase and cellulase at the same time, wherein the pectinase is 0.6 percent and the cellulase is 0.05 percent, the pH value is adjusted to be 3.1, the enzymolysis temperature is 52 ℃, the enzymolysis time is 160min, after the enzymolysis is finished, the enzyme is inactivated for 30min at the temperature of 90 ℃, filtering by a 200-mesh sieve to collect filtrate, concentrating the filtrate into semi-flowing pulp, and then mixing the pulp with the ratio of 1: dissolving the mixture in deionized water at a volume ratio of 10, stirring and shaking up to obtain the longan extract.
The preparation method of the Guangxi sweet tea fruit extract comprises the following steps: weighing Guangxi sweet tea peel and pulp, placing in a mortar, adding 85% methanol solution, grinding, completely transferring to a container with a plug, ultrasonically extracting for 50 min at 40 deg.C and 200W ultrasonic power, cooling at room temperature, and centrifuging the extractive solution at 4500 rpm/min for 10 min; after concentrating the supernatant from the centrifugation to a semi-fluid slurry, the slurry was mixed at a ratio of 1: dissolving the extract in deionized water at a volume ratio of 10, stirring and shaking uniformly to obtain the Guangxi rubus suavissimus fruit extract.
The preparation methods of the ficus pumila extract, the momordica grosvenori extract and the apple extract comprise the following steps: respectively adding 90% ethanol solution into peel and pulp of various raw materials pulverized by liquid nitrogen at a solid-liquid ratio of 1:33, and ultrasonically extracting at 31 deg.C and 200W for 38min to obtain extractive solution; filtering the extractive solution, and concentrating under reduced pressure to obtain slurry; the slurry was mixed at a ratio of 1: dissolving 10 volume ratio in deionized water, stirring, and shaking to obtain Ficus pumila Linn hidden fruit extract, fructus Siraitiae Grosvenorii extract and fructus Mali Pumilae extract.
The preparation method of the indigo fruit extract comprises the following steps: washing away silt on the surface of the lonicera edulis by flowing water, introducing an ozone generator into sterile water to wash the lonicera edulis again, then draining and removing the core, crushing pulp into pulp, adding deionized water according to 40% of the volume of the pulp, simultaneously adding 3.5% of pectinase, stirring uniformly, and standing for 30min at 35 ℃; adding the supernatant into 95% ethanol solution, ultrasonic extracting at 40 deg.C and 200W ultrasonic power for 15min, and centrifuging at 4000 rpm/min for 10 min; after concentrating the supernatant from the centrifugation to a semi-fluid slurry, the slurry was mixed at a ratio of 1: dissolving the mixture in deionized water according to the volume ratio of 10, stirring and shaking up to obtain the lonicera edulis extract.
The preparation method of the mango extract comprises the following steps: weighing mango pulp and peel freeze-dried powder, adding 80% acetone at a solid-to-liquid ratio of 1:20, ultrasonically extracting for 30min at an ultrasonic frequency of 60kHz and a power of 30W, centrifuging for 10min at 7000rpm/min, taking supernatant, repeating the above operations twice, and combining the supernatants; and (3) evaporating the combined supernatant on a rotary evaporator to dryness, and mixing the product obtained after evaporation to dryness in a ratio of 1: dissolving the mixture in deionized water at a volume ratio of 10, stirring and shaking up to obtain the mango extract.
The preparation method of the freeze-dried powder of the composition with the acne removing effect in the embodiment is characterized by comprising the following steps:
the method comprises the following steps: putting polyglutamic acid and deionized water into a homogenizer according to a proportion, homogenizing for 10min at 78 ℃, completely dissolving to obtain A, and then placing A for natural cooling;
step two: when the temperature of A is reduced to about 50 ℃, mannitol and dextran are added according to the proportion and are uniformly mixed to obtain B;
step three: when the temperature of the B is reduced to about 30 ℃, adding the traditional Chinese medicine extract, the ficus pumila cryptocephala extract, the momordica grosvenori extract, the lonicera edulis extract, the Guangxi rubus suavissimus fruit extract, the mango extract, the longan extract, the apple extract, the nano-graphene and the fibroblast growth factor in proportion, uniformly stirring to obtain C, then freezing the C at-25 ℃ for 20 hours, and discharging after the plate alignment is qualified;
step four: filling the frozen C, placing the C in a vacuum freeze drying device, and carrying out vacuum freeze drying treatment for 35h at the temperature of-50 ℃ and the vacuum degree of 7Pa to obtain the freeze-dried powder containing the composition with the acne removing effect.
The composition with the acne removing effect and the preparation method of the freeze-dried powder thereof have the following effects:
the application method of the freeze-dried powder comprises the following steps: firstly, the solvent is extracted, and injected into an ampoule bottle filled with freeze-dried powder, and the bottle is shaken to ensure that the freeze-dried powder and the solvent are completely mixed to prepare freeze-dried powder solution. One bottle of the freeze-dried powder solution is used once a day.
Human skin irritation test
100 male and female aged 18-28 are selected respectively, after the medicine is continuously used for 10 days, whether skin is red, swollen or not is observed, and a feedback test result after 10 days is that 200 test subjects have no red, swollen or edema phenomenon, which shows that the freeze-dried powder of the invention has no stimulation to the skin and can be suitable for people with various skins.
Detection of acne-removing Effect
300 patients with face acne in 18-28 years old are selected for continuous trial for 30 days, and then divided into three groups of 100 people to feed back acne and acne removing effects.
Wherein, the three groups of people who have obvious effect, namely feel that the acne and the pox are obviously lightened are respectively 75, 80 and 77; three groups of people who are effective, namely, people who feel the acne and the pox relieved and improved by themselves are 25 people, 20 people and 23 people respectively; three groups of people who are ineffective, i.e., who find themselves with little or no visible reduction in acne or pox are 0. From the detection results, the significant effects, i.e., the significant efficiencies of the three groups were 75%, 80% and 77%, respectively, and the average significant efficiency of the three groups was 77.3%. The composition with the acne removing effect and the preparation method of the freeze-dried powder thereof have excellent acne removing effect. Meanwhile, if the Chinese medicinal composition is used for a long time, the Chinese medicinal composition can also effectively resist external and self oxidation factors, and resist pigmentation, and formation of pockmarks and pox scars. The test samples in the present invention were the samples prepared in example 3 of the present invention.
The composition with the acne removing effect and the preparation method of the freeze-dried powder thereof and the multiple fruit extracts are a composite action system, and comprehensively utilize the effects of taking longan extracts as the first, Ficus pumila cravings fruit extracts and mango extracts as auxiliary synergistic anti-inflammatory components, the effects of antibacterial and antiviral components of fructus momordicae extracts and Guangxi beet fruit extracts, the effects of special light scar healing promoting components of indigo honeysuckle extracts and apple extracts, the effect of an antiseptic component of the common effect of the apple extracts and the Guangxi beet fruit extracts, and the Ficus pumila crassipes fruit extracts as a natural stabilizer. The components of the whole natural fruit extract can bring out the best in each other aiming at the problems of acne and pox. The invention combines the advantages of various raw materials, such as sufficient and perfect efficacy, and more efficiently plays the overall effects of continuous antibiosis, anti-inflammation, acne removal, scar lightening and cell proliferation promotion to regenerate the skin. According to the composition with the acne removing effect and the preparation method of the freeze-dried powder thereof, the biological transdermal enhancer is derived from the plant fruit extract, so that the composition has good biocompatibility, cannot damage the skin, and has safety.
The present invention has been described in connection with the specific embodiments, and it is to be understood that the invention is not limited to the above-described embodiments, and various modifications may be made without departing from the spirit and scope of the invention.
Claims (1)
1. A composition with acne removing efficacy is characterized in that: the composition comprises the following components in parts by weight: 10 parts of traditional Chinese medicine extract, 3-5 parts of ficus pumila cryptocephala extract, 3-5 parts of momordica grosvenori extract, 4-6 parts of lonicera edulis extract, 2-4 parts of cantonese rubus suavissima fruit extract, 6-8 parts of mango extract, 6-8 parts of longan extract, 10-12 parts of apple extract, 0.5 part of nano graphene, 0.01 part of fibroblast growth factor, 1-2 parts of dextran, 0.05-0.1 part of polyglutamic acid, 2-4 parts of mannitol and 65-85 parts of deionized water; the traditional Chinese medicine extract comprises the following components in parts by weight: 5 parts of licorice extract and 5 parts of salvia miltiorrhiza extract;
putting polyglutamic acid and deionized water into a homogenizer according to a proportion, homogenizing for 10min at 78 ℃, completely dissolving to obtain A, and then placing A for natural cooling;
when the temperature of the A is reduced to 50 ℃, mannitol and dextran are added according to the proportion and are uniformly mixed to obtain B;
when the temperature of the B is reduced to 30 ℃, adding the traditional Chinese medicine extract, the ficus pumila cryptocephala extract, the momordica grosvenori extract, the lonicera edulis extract, the Guangxi rubus suavissimus fruit extract, the mango extract, the longan extract, the apple extract, the nano graphene and the fibroblast growth factor in proportion, uniformly stirring to obtain C, then freezing the C at the temperature of minus 25 ℃ for 20 hours, and discharging after the plate alignment is qualified; filling the frozen C, placing the C in vacuum freeze drying equipment, and carrying out vacuum freeze drying treatment for 35h at the temperature of-50 ℃ and the vacuum degree of 7Pa to obtain freeze-dried powder containing the composition with the acne removing effect;
the preparation method of the longan extract adopts a pectinase and cellulase double-enzyme simultaneous enzymolysis extraction method, and comprises the following specific steps: drying fresh longan with hot air at 80 deg.C, peeling, removing core to obtain dried arillus longan, sealing, and storing at 4 deg.C; weighing dried longan pulp, adding deionized water, soaking for 12h with a material-water ratio of 1:3, and mashing with a high-speed tissue mashing machine; adding deionized water into the smashed material, wherein the material-water ratio is 1:12, performing enzymolysis by using two enzymes of pectinase and cellulase at the same time, wherein the pectinase is 0.6 percent and the cellulase is 0.05 percent, the pH value is adjusted to be 3.1, the enzymolysis temperature is 52 ℃, the enzymolysis time is 160min, after the enzymolysis is finished, the enzyme is inactivated for 30min at the temperature of 90 ℃, filtering by a 200-mesh sieve to collect filtrate, concentrating the filtrate into semi-flowing pulp, and then mixing the pulp with the ratio of 1: dissolving the mixture in deionized water at a volume ratio of 10, stirring and shaking up to obtain longan extract;
the preparation method of the Guangxi rubus suavissimus fruit extract comprises the following steps: weighing Guangxi sweet tea peel and pulp, placing in a mortar, adding 85% methanol solution, grinding, completely transferring to a container with a plug, ultrasonically extracting for 50 min at 40 deg.C and 200W ultrasonic power, cooling at room temperature, and centrifuging the extractive solution at 4500 rpm/min for 10 min; after concentrating the supernatant from the centrifugation to a semi-fluid slurry, the slurry was mixed at a ratio of 1: dissolving the mixture in deionized water according to the volume ratio of 10, stirring and shaking uniformly to obtain the Guangxi rubus suavissimus fruit extract;
the preparation methods of the cryptocarpus ficus pumila extract, the momordica grosvenori extract and the apple extract comprise the following steps: respectively adding 90% ethanol solution into peel and pulp of various raw materials pulverized by liquid nitrogen at a solid-liquid ratio of 1:33, and ultrasonically extracting at 31 deg.C and 200W for 38min to obtain extractive solution; filtering the extractive solution, and concentrating under reduced pressure to obtain slurry; the slurry was mixed at a ratio of 1: dissolving 10 volume ratios of the extracts in deionized water, stirring and shaking up to obtain the cryptomeria japonica extract, the momordica grosvenori extract and the apple extract respectively;
the preparation method of the lonicera edulis extract comprises the following steps: washing away silt on the surface of the lonicera edulis by flowing water, introducing an ozone generator into sterile water to wash the lonicera edulis again, then draining and removing the core, crushing pulp into pulp, adding deionized water according to 40% of the volume of the pulp, simultaneously adding 3.5% of pectinase, stirring uniformly, and standing for 30min at 35 ℃; adding the supernatant into 95% ethanol solution, ultrasonic extracting at 40 deg.C and 200W ultrasonic power for 15min, and centrifuging at 4000 rpm/min for 10 min; after concentrating the supernatant from the centrifugation to a semi-fluid slurry, the slurry was mixed at a ratio of 1: dissolving the mixture in deionized water at a volume ratio of 10, stirring and shaking up to obtain the lonicera edulis extract;
the preparation method of the mango extract comprises the following specific steps: weighing mango pulp and peel freeze-dried powder, adding 80% acetone at a solid-to-liquid ratio of 1:20, ultrasonically extracting for 30min at an ultrasonic frequency of 60kHz and a power of 30W, centrifuging for 10min at 7000rpm/min, taking supernatant, repeating the above operations twice, and combining the supernatants; and (3) evaporating the combined supernatant on a rotary evaporator to dryness, and mixing the product obtained after evaporation to dryness in a ratio of 1: dissolving the mixture in deionized water at a volume ratio of 10, stirring and shaking up to obtain the mango extract.
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