CN106619150B - The natural sun-prevention moisturizing lipidosome freeze-dried powder of γ-PGA modification and its preparation and application - Google Patents
The natural sun-prevention moisturizing lipidosome freeze-dried powder of γ-PGA modification and its preparation and application Download PDFInfo
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Abstract
The invention discloses a kind of natural sun-prevention moisturizing lipidosome freeze-dried powders of γ-PGA modification, by mass percentage include following components: rutin and pineapple leaves extract 2~5%, phosphatidase 1 5~35%; cholesterol 5~10%; positive charge control agent 2~8%, Tween-803~12%, VE 0.5~2%; VC 0.5~2%; beta glucan 1~14%, gamma-polyglutamic acid 1~3%, freeze drying protectant 20~60%; the quality summation of water≤5%, the above components is 100%.Preparation method is to use liposomal encapsulated rutin and pineapple leaves extract, and is prepared into DPPC Thermo-sensitive liposome, then gamma-polyglutamic acid is used to modify liposome.It can directly be made an addition in cosmetics after lipidosome freeze-dried powder aquation of the invention, sun-proof and energy moisturizing, sharp property is low, and Transdermal absorption effect is good, and the rate of release of effective component is big.
Description
Technical field
The invention belongs to cosmetic material preparation technical fields, are related to a kind of day of γ-PGA (gamma-polyglutamic acid) modification
Right sun-proof moisturizing lipidosome freeze-dried powder, the invention further relates to the preparation method and application of the liposome.
Background technique
Ozone layer it is continuous thinning, cause reach ground ultraviolet radiation (UVR) enhanced strength, excessive exposure ultraviolet light
(UV) the problems such as causing sunburn, pigmentation, solar dermatitis or even cutaneum carcinoma.For this purpose, antitan agent has caused widely to close
Note.
Sunscreen product in the market is mostly to reach broad spectrum ultraviolet light by addition physical sunscreen agent and chemical sun to prevent
The effect of shield, but the problems such as physical sunscreen agent there are protective capacities weak, easy blocking pore, then there is photostability in chemical sun
The problems such as poor, irritant.In recent years with the natural plant composition of sun-screening function, not only there is excellent sun-proof performance, and
And people are met in safety, the demand of environmentally protective aspect.
Existing scholar screens and develops to natural sun-screening agents at present, and is directly appended in cosmetics, still
It is influenced by self stability etc., and Transdermal absorption is undesirable；Most of sunscreen cosmetic ingredient is single, and broad absorption is not achieved
The effect of ultraviolet light；And the moistening effect of most of sunscreen cosmetic is not satisfactory.
Summary of the invention
It is an object of the present invention to provide a kind of gamma-polyglutamic acid modification natural sun-prevention moisturizing lipidosome freeze-dried powder,
The ultraviolet absorbing agent of liposomal encapsulated two kinds of complementary types, solves that existing sunscreen product ingredient is single, stability is poor, function
Single problem.
It is a further object to provide the natural sun-prevention moisturizing lipidosome freeze-dried powders of above-mentioned gamma-polyglutamic acid modification
Preparation method.
Third object of the present invention is to provide the natural sun-prevention moisturizing lipidosome freeze-dried powders of above-mentioned gamma-polyglutamic acid modification
Application.
First technical solution that the present invention uses is a kind of natural sun-prevention moisturizing liposome of gamma-polyglutamic acid modification
Freeze-dried powder includes following components: rutin and pineapple leaves extract 2~5%, phosphatidase 1 5~35%, cholesterol by mass percentage
5~10%, positive charge control agent 2~8%, Tween-803~12%, VE 0.5~2%, VC 0.5~2%, beta glucan 1
~14%, the quality summation of gamma-polyglutamic acid 1~3%, freeze drying protectant 20~60%, water≤5%, the above components is
100%.
Preferably, phosphatide be dipalmitoylphosphatidylcholine (DPPC) or with dimyristoyl phosphatidyl choline (DMPC),
A kind of mixture of hydrolecithin (HSPC), lysophosphatide (MSPC), dipalmitoylphosphatidylglycerol (DPPG) etc.；Freeze-drying
Protective agent is trehalose and mannitol mixture；Positive charge control agent is octadecylamine, phosphatidyl-ethanolamine or dialkyl ether ammonium salt
One or more of；
Preferably, the mass ratio of rutin and pineapple leaves extract is 1:1~3:1.
Another technical solution of the invention is that the natural sun-prevention moisturizing of above-mentioned gamma-polyglutamic acid modification is lipidosome freeze-dried
The preparation method of powder, following steps:
Step 1, raw material components are weighed by following mass percent: rutin and pineapple leaves extract 2~5%, phosphatidase 1 5~
35%, cholesterol 5~10%, positive charge control agent 2~8%, T ween-803~12%, VE 0.5~2%, VC 0.5~
2%, beta glucan 1~14%, gamma-polyglutamic acid 1~3%, freeze drying protectant 20~60%, the quality summation of the above components
It is 100%.
Step 2: phosphatide, cholesterol, VE, Tween-80, positive charge control agent, rutin and pineapple leaves extract are dissolved in nothing
Water-ethanol obtains A liquid, A liquid rotary evaporation is removed dehydrated alcohol, and form one layer of lipid membrane on the wall.
Step 3: VC, beta glucan and freeze drying protectant being dissolved in phosphate buffer solution (PBS), obtain B liquid.
Step 4: in the obtained lipid membrane of B solution addition step 2, will make film separation, after abundant aquation, obtain liposome
Suspension C liquid.
Step 5: gamma-polyglutamic acid being dissolved in water, and liposome turbid liquor C liquid is added, obtains gamma-polyglutamic acid after centrifugation
The natural sun-prevention moisturizing liposome of modification, then gamma-polyglutamic acid modification is successively obtained after film whole grain, freeze thawing, freeze-drying
Natural sun-prevention moisturizing lipidosome freeze-dried powder.
Preferably, 30~40 DEG C of step 2 rotating evaporation temperature, 0.008~0.01Mpa of vacuum degree.
Preferably, gamma-polyglutamic acid molecular weight is 70~1,000,000 in step 5, and gamma-polyglutamic acid concentration of aqueous solution is
0.1%, pH are 5~6.
Preferably, step 5 crosses film whole grain are as follows: successively passes through 0.8 μm of filter membrane, 0.65 μm of filter membrane, 0.45 μm of filter membrane and 0.22
μm filter membrane carries out whole grain.
Preferably, step 5 freeze thawing treatment process are as follows: freeze 30~60min first in -60 DEG C of environment, be subsequently placed in 15
Make its thawing in~20 DEG C of environment, is ultrasonically treated 1min, circular treatment 3~4 after the completion of thawing in 15~20 DEG C of environment
It is secondary.
Preferably, step 5 freeze-drying process process are as follows: under -60 ± 1 DEG C of environmental condition after pre-freeze 10h, then -52
± 2 DEG C, vacuum degree less than 100mT environmental condition under be freeze-dried 25h.
The present invention also provides the natural sun-prevention moisturizing lipidosome freeze-dried powders of above-mentioned gamma-polyglutamic acid modification in cosmetics
Application.
When preparing sunscreen cosmetic, deionized water aquation is added to the liposome of the encapsulating sun-prevention component of above-mentioned γ-PGA modification
It is then directly added into cosmetics afterwards, can be applied to a variety of State of cosmetics such as lotion, sun-proof creams, spraying, facial mask or aqua.
Additive amount of the liposome of the encapsulating sun-prevention component of γ-PGA modification in sunscreen cosmetic is 0.2~15%.
The present invention selects rutin and pineapple leaves extract as ultraviolet absorbing agent, absorption activity of the rutin to UVA, UVC
Greatly, pineapple leaves extract is big to the absorption activity of UVB, UVC, since two kinds of active components compounding has ultraviolet radiation absorption activity
Two kinds are used in mixed way the effect that can reach broad absorption ultraviolet light by complementarity.And directly addition uses, light in environment,
The factors such as heat, oxygen and metal ion in system and pH value, seriously affect its stability and percutaneous absorbability.
Active constituent or drug are wrapped up using liposome technology and are prepared into nano liposomes by the present invention, can promote
Skin absorbs.Liposome is the completely enclosed multi-layer vesicles of the bilayer formed by lipoids such as phosphatide, cholesterol.For making up
The liposome of product can encapsulate the substance of stability difference, improve the irritation of raw material, controllable and safe and non-toxic with targeting, being sustained
The characteristics of, while the phospholipid bilayer of liposome may also help in during effective component preferably penetrates to the skin, it is wide
It is general to be applied in cosmetics.
The present invention joined dipalmitoylphosphatidylcholine (DPPC) in membrane material, and conventional liposome is prepared into DPPC temperature
Quick property liposome, when ambient temperature reaches at or above phase transition temperature (Tm) of membrane material, phospholipid bilayer is by " gel " state knot
Structure changes to " liquid crystal " state structure, and the acyl chain turbulence increase of hydrophobic side results in phosphatide, and the mobility of liposome membrane increases
Greatly, the drug of lipid core or the release speed of active constituent and the drug being embedded in inside bilayer or active constituent are encapsulated in
Rate increases, performance its effect that can be bigger.
The present invention also uses gamma-polyglutamic acid to modify liposome.Gamma-polyglutamic acid (γ-PGA) is a kind of
Polycarboxylic acid has extremely strong moisture retention.Cosmetics containing γ-PGA can form thin film on the skin, prevent moisture
Evaporation, or even the self-regeneration function of skin can also be restored.The effective component that positive charge is taken in gamma-polyglutamic acid and liposome is sent out
Raw molecular action makes effective component slow release and act on skin within the relatively long period, is conducive to its slow-release capability
Skin fully absorbs, and plays the effect of effective component.
The beneficial effects of the present invention are:
(1) natural sun-prevention moisturizing lipidosome freeze-dried powder of the invention encloses the ultraviolet absorbing agent of two kinds of complementary types, can
To reach the efficient absorption of ultraviolet light.
(2) irritation is low, and Transdermal absorption effect is good, solves and directly makes an addition in cosmetics there are certain irritation,
The problems such as stability is poor, and Transdermal absorption ability is not satisfactory；
(3) rate of release of the sun-proof moisturizing active constituent of the present invention when temperature reaches a certain level is big, hair that can be bigger
Wave its effect.
(4) moisture-keeping efficacy can also be played while effective protection skin sunburn of the present invention.
Detailed description of the invention
Fig. 1 is natural sun-prevention moisturizing lipidosome freeze-dried powder preparation method flow chart of the present invention；
Fig. 2 is invented liposomes freeze-dried powder grain size distribution；
Fig. 3 is that the liposome transdermal of natural sun-prevention component of the present invention absorbs figure；
Fig. 4 is that the liposome Cumulative release amount of natural sun-prevention component of the present invention varies with temperature figure；
Fig. 5 is the liposome ultraviolet absorbance figure of natural sun-prevention component of the present invention.
Specific embodiment
Present invention will be described in further detail below with reference to the accompanying drawings and specific embodiments, but the present invention is not limited to
These embodiments.
Embodiment 1
Preparation method is as shown in Figure 1.
Step 1: weigh respectively dipalmitoylphosphatidylcholine (DPPC) 2.40g, cholesterol 0.80g, VE0.06g,
Tween-80 0.30g, octadecylamine 0.36g, rutin 0.12g, pineapple leaves extract 0.12g are dissolved in 42mL dehydrated alcohol and obtain A liquid；
Step 2: the solution A that step 1 is obtained is in 35 DEG C on Rotary Evaporators, and the evaporation of 0.009Mpa rotating pressure-decreasing is except anhydrous
Ethyl alcohol forms one layer of lipid membrane；
Step 3: beta glucan 0.58g, VC 0.08g, trehalose 1.6g, mannitol 1.6g are dissolved in 129mL pH=7.0
Phosphate buffer solution obtain B liquid；Decompression rotary evaporation 50min, which is added, in B liquid makes film separation, after abundant aquation, 35 DEG C of water
Ultrasound 15min obtains liposome turbid liquor in bath.
Step 4: the gamma-polyglutamic acid aqueous solution that compound concentration is 0.1% is 5 with NaOH tune pH, stirs 20min；
Step 5: the liposome solutions of the encapsulating natural sun-prevention component of step 3 are added to the polyglutamic acid solution of step 4, centrifugation
The natural sun-prevention moisturizing liposome of gamma-polyglutamic acid modification is obtained afterwards；
Step 6: successively carrying out whole grain by 0.8 μm of filter membrane, 0.65 μm of filter membrane, 0.45 μm of filter membrane and 0.22 μm of filter membrane.
Step 7: freeze 60min in -60 DEG C of environment, being subsequently placed in 15 DEG C of environment makes its thawing, after the completion of thawing
It is ultrasonically treated 1min in 15 DEG C of environment, circular treatment 3 times, is distributed into cillin bottle the pre-freeze under -60 ± 1 DEG C of environmental condition
After 10h, then -52 ± 2 DEG C, vacuum degree less than 100mT environmental condition under be freeze-dried 25h, obtain gamma-polyglutamic acid modification
Natural sun-prevention moisturizing lipidosome freeze-dried powder.
Its constituent mass percentage of the natural sun-prevention moisturizing lipidosome freeze-dried powder of the modification of gamma-polyglutamic acid made from the present embodiment
Than for rutin and pineapple leaves extract 2.81%, phosphatidase 2 8.10%, cholesterol 9.37%, positive charge control agent 4.21%,
Tween-80 3.51%, VE 0.70%, VC 0.94%, beta glucan 6.79%, gamma-polyglutamic acid 2.00%, frozen-dried protective
Agent 37.47%, water 4.10%.
Embodiment 2
Step 1: weighing dipalmitoylphosphatidylcholine (DPPC) and dipalmitoylphosphatidylglycerol (DPPG) 4.90g
(DPPC:DPPG=10:1), cholesterol 1.30g, VE 0.10g, Tween-80 0.40g, two-octadecane-two-methyl amine salt
0.60g, rutin 0.30g, pineapple leaves extract 0.20g are dissolved in 78mL dehydrated alcohol and obtain A liquid；
Step 2: the solution A that step 1 is obtained is in 40 DEG C on Rotary Evaporators, and the evaporation of 0.01Mpa rotating pressure-decreasing is except anhydrous
Ethyl alcohol forms one layer of lipid membrane；
Step 3: beta glucan 0.50g, VC 0.10g, trehalose 3.2g, mannitol 3.2g are dissolved in 233mL pH=
6.9 phosphate buffer solution obtains B liquid；Decompression rotary evaporation 50min, which is added, in B liquid makes film separation, after abundant aquation, 35 DEG C
Ultrasound 15min obtains liposome turbid liquor in water-bath.
Step 4: the gamma-polyglutamic acid aqueous solution that compound concentration is 0.1% is 5.5 with NaOH tune pH, stirs 20min；
Step 5: the liposome solutions of the encapsulating natural sun-prevention component of step 3 are added to the gamma-polyglutamic acid solution of step 4,
The natural sun-prevention moisturizing liposome of gamma-polyglutamic acid modification is obtained after centrifugation；
Step 6: successively carrying out whole grain by 0.8 μm of filter membrane, 0.65 μm of filter membrane, 0.45 μm of filter membrane and 0.22 μm of filter membrane.
Step 7: freeze 60min in -60 DEG C of environment, being subsequently placed in 18 DEG C of environment makes its thawing, after the completion of thawing
It is ultrasonically treated 1min in 18 DEG C of environment, circular treatment 3 times, is distributed into cillin bottle the pre-freeze under -60 ± 1 DEG C of environmental condition
After 10h, then -52 ± 2 DEG C, vacuum degree less than 100mT environmental condition under be freeze-dried 25h, obtain gamma-polyglutamic acid modification
Natural sun-prevention moisturizing lipidosome freeze-dried powder.
Its constituent mass percentage of the natural sun-prevention moisturizing lipidosome freeze-dried powder of the modification of gamma-polyglutamic acid made from the present embodiment
Than for rutin and pineapple leaves extract 3.24%, phosphatidase 3 2.42%, cholesterol 8.43%, positive charge control agent 3.87%,
Tween-80 2.59%, VE 0.65%, VC 0.65%, beta glucan 3.24%, gamma-polyglutamic acid 2.02%, frozen-dried protective
Agent 41.50%, water 1.36%.
Embodiment 3
Step 1: weighing dipalmitoylphosphatidylcholine (DPPC) and dimyristoyl phosphatidyl choline (DMPC) 1.60g
(DPPC:DMPC=10:1), cholesterol 0.46g, VE 0.18g, Tween-80 1.00g, dioleoylphosphatidylethanolamine
(DOPE) 0.60g, rutin 0.12g, pineapple leaves extract 0.04g are dissolved in 40mL dehydrated alcohol and obtain A liquid；
Step 2: the solution A that step 1 is obtained is in 30 DEG C on Rotary Evaporators, and the evaporation of 0.008Mpa rotating pressure-decreasing is except anhydrous
Ethyl alcohol forms one layer of lipid membrane；
Step 3: beta glucan 1.10g, VC 0.18g, trehalose 1.6g, mannitol 1.6g are dissolved in 150 mL pH=
6.8 phosphate buffer solution obtains B liquid；Decompression rotary evaporation 50min, which is added, in B liquid makes film separation, after abundant aquation, 35 DEG C
Ultrasound 15min obtains liposome turbid liquor in water-bath；
Step 4: the gamma-polyglutamic acid aqueous solution that compound concentration is 0.1% is 6 with NaOH tune pH, stirs 20min；
Step 5: the liposome of the encapsulating natural sun-prevention component of step 3 is added to the polyglutamic acid solution of step 4, after centrifugation
The natural sun-prevention moisturizing liposome modified to gamma-polyglutamic acid；
Step 6: successively carrying out whole grain by 0.8 μm of filter membrane, 0.65 μm of filter membrane, 0.45 μm of filter membrane and 0.22 μm of filter membrane.
Step 7: freeze 60min in -60 DEG C of environment, being subsequently placed in 20 DEG C of environment makes its thawing, after the completion of thawing
It is ultrasonically treated 1min in 20 DEG C of environment, circular treatment 3 times, is distributed into cillin bottle the pre-freeze under -60 ± 1 DEG C of environmental condition
After 10h, then -52 ± 2 DEG C, vacuum degree less than 100mT environmental condition under be freeze-dried 25h, obtain gamma-polyglutamic acid modification
Natural sun-prevention moisturizing lipidosome freeze-dried powder.
Its constituent mass percentage of the natural sun-prevention moisturizing lipidosome freeze-dried powder of the modification of gamma-polyglutamic acid made from the present embodiment
Than for rutin and pineapple leaves extract 1.79%, phosphatidase 1 7.94%, cholesterol 1.79%, positive charge control agent 6.73%,
Tween-80 11.21%, VE 2.02%, VC 2.02%, beta glucan 12.33%, gamma-polyglutamic acid 2.13%, freeze-drying are protected
Protect agent 35.87%, water 2.80%.
Through detecting, the average grain diameter of invented liposomes suspension is 280 ± 30nm, as shown in Figure 2.
The effect of investigating the natural sun-prevention moisturizing liposome of gamma-polyglutamic acid prepared by the present invention modification, with embodiment 1,
For the lipidosome freeze-dried powder of embodiment 2 and embodiment 3, the detection of following aspect is carried out:
1. temperature sensitivity detects
Test method are as follows: the lipidosome freeze-dried powder in Example 1 takes 10mL to be placed in bag filter after being dissolved in water in right amount, puts
It is discharged in physiological saline, 35 DEG C to 44 DEG C of temperature range, discharges 20min in the case of each set temperature, take a random sample respectively
It is measured and is detected with HPLC, obtain drug Cumulative release amount.As shown in figure 3, it can be seen from the figure that living in 35 DEG C to 39 DEG C
Property ingredient release have almost no change, when temperature within the scope of 39 DEG C to 42 DEG C burst size increase, as temperature continues to increase,
Burst size tends to be steady.Quick release of the active constituent at 39 DEG C is attributed to 38.13 DEG C of first phase transition temperature, when in temperature
Burst size tends to be steady when rising to 42 DEG C is determined by 41.99 DEG C of its second phase transition temperature, is discharged when within the scope of 35 DEG C to 39 DEG C
Amount varies with temperature only slight increase, and the reason of such case occur is that release temperature is lower than temperature sensitive phase change temperature of liposome,
The permeance property of phospholipid bilayer only slightly changes.
2. percutaneous abilities detect
Sample sets be 1 lipidosome freeze-dried powder of embodiment, reference substance be prepare concentration same as Example 1 sun-prevention component it is molten
Liquid distinguishes the sun-prevention component being in reference substance without liposome.
Test method are as follows: tested using isolated skin Transdermal absorption, in vitro rabbit in homogeneous thickness is chosen, with physiological saline
It rinses well, freezen protective.Using Franz transdermal diffusion apparatus, rabbit is placed between the room up and down of Franz diffusion cell, cutin
Layer upward, takes test sample to be applied to skin surface, and phosphate buffer solution is contained in reception tank, and built-in stirrer keeps temperature 36
±1℃.Respectively at 0.5,2,4,6,8,10,12, the whole receiving liquids of taking-up for 24 hours, and isometric receiving liquid is supplemented rapidly.It utilizes
High performance liquid chromatograph is detected, and calculates Percutaneous permeability Q (the μ g/cm of sun-prevention component2)。
Test concrete outcome is shown in Fig. 4, as can be seen from the figure the natural sun-prevention moisturizing liposome after gamma-polyglutamic acid modification
It is apparently higher than the transdermal effect of the sun-prevention component without liposome in each sample point Transdermal absorption effect, illustrates γ-
Liposome after polyglutamic acid modification can improve transdermal the problem of being obstructed.
3. sun-proof function is tested
A, sun-proof spf value measurement
Test object is two comparative examples of three embodiments of the invention and setting, two comparative example preparation steps and item
Part is same as Example 3, the difference is that, rutin 0.16g is added in 1 step of comparative example (1), pineapple leaves extract is not added,
Pineapple leaves extract 0.16g is added in step (1) in comparative example 2, and rutin is not added.
Using the portable spectrometer Optometrics LLC SPF-290S of u s company Optometrics production in room
It inside measures, in order to guarantee measurement accuracy when measurement, operating process is carried out in strict accordance with measuring process as defined in the instrument:
Test environment conditions: thermostatic constant wet chamber, 22 DEG C ± 1 DEG C of temperature, humidity 50% ± 10%.
Appropriate standard specimen or sample (2mg/cm are drawn with one-shot injector2), by it, uniformly point puts on latex on PMMA plate
Finger-cover for medical use is smeared uniformly, places 30min in dark.After preheater apparatus 15min, it is inserted into blank plate, adjusts light intensity and maximum wave
It grows to through instrument preliminary examination, is subsequently inserted into standard specimen and sample panel, randomly select 8 points of tests, obtain MPF curve, it is mating by instrument
Sample spf value is calculated in software.Test concrete outcome is shown in Table 1.
The measurement of 1 sample spf value of table
From table 1 as can be seen that the SPF value for the natural sun-prevention liposome that gamma-polyglutamic acid prepared by the present invention is modified compared with
The effect of height of single sun-prevention component, a combination thereof, is obviously improved, and has certain protection effect.
B, ultraviolet radiation absorption determination of activity
Liposome accurate weighing 100mg obtained by embodiment 3 and comparative example 1, comparative example 2 is dissolved in 50mL volumetric flask
In, dehydrated alcohol is added as solvent, and solution is made.By manufactured solution down in 1cm × 1cm × 5cm quartz cuvette pond,
The wave-length coverage ultraviolet absorbance of 200~400nm is measured in the ultra-violet and visible spectrophotometer debugged.Test knot
Fruit is as shown in figure 5, it can be seen from the figure that the only encapsulating pineapple leaves extract liposome of preparation is in UVA mean light absorbency than making
The liposome of standby only encapsulating rutin is low in UVA mean absorbance values, and in the mean absorbance values in the section UVB, UVC than only wrapping
The liposome for sealing rutin is high, and what is prepared is absorbing purple using rutin and pineapple leaves extract as the liposome of ultraviolet absorbing agent
Outside line effect is more preferably compared with single component.
Claims (8)
1. a kind of natural sun-prevention moisturizing lipidosome freeze-dried powder of γ-PGA modification, which is characterized in that include by mass percentage with
Lower component: rutin and pineapple leaves extract 2~5%, phosphatidase 1 5~35%, cholesterol 5~10%, positive charge control agent 2~
8%, Tween-803~12%, VE 0.5~2%, VC 0.5~2%, beta glucan 1~14%, gamma-polyglutamic acid 1~
3%, the quality summation of freeze drying protectant 20~60%, water≤5%, the above components is 100%；Wherein, phosphatide is two palms
Phosphatidyl choline, or be dimyristoyl phosphatidyl choline, hydrolecithin, lysophosphatide, dipalmitoylphosphatidylglycerol
In any mixture with dipalmitoylphosphatidylcholine；Freeze drying protectant is trehalose and mannitol mixture；Positive charge
Controlling agent is one or more of octadecylamine, phosphatidyl-ethanolamine or dialkyl ether ammonium salt；Rutin and pineapple leaves extract
Mass ratio is 1:1~3:1.
2. the preparation method of the natural sun-prevention moisturizing lipidosome freeze-dried powder of γ-PGA modification as described in claim 1, feature exist
In following steps:
Step 1, raw material components are weighed by following mass percent: rutin and pineapple leaves extract 2~5%, phosphatidase 1 5~35%,
Cholesterol 5~10%, positive charge control agent 2~8%, Tween-803~12%, VE0.5~2%, VC 0.5~2%, β-Portugal
The quality summation of glycan 1~14%, gamma-polyglutamic acid 1~3%, freeze drying protectant 20~60%, the above components is 100%；
Step 2: by phosphatide, cholesterol, VE, Tween-80, positive charge control agent, rutin and pineapple leaves extract are dissolved in anhydrous second
Alcohol obtains A liquid, A liquid rotary evaporation is removed dehydrated alcohol, and form one layer of lipid membrane on the wall；
Step 3: VC, beta glucan and freeze drying protectant being dissolved in phosphate buffer solution (PBS), obtain B liquid；
Step 4: in the obtained lipid membrane of B solution addition step 2, will make film separation, after abundant aquation, obtain liposome suspension
Liquid C liquid；
Step 5: gamma-polyglutamic acid being dissolved in water, and liposome turbid liquor C liquid is added, gamma-polyglutamic acid modification is obtained after centrifugation
Natural sun-prevention moisturizing liposome, then successively obtained after film whole grain, freeze thawing, freeze-drying gamma-polyglutamic acid modification day
Right sun-proof moisturizing lipidosome freeze-dried powder.
3. the preparation method of the natural sun-prevention moisturizing lipidosome freeze-dried powder of γ-PGA modification according to claim 2, special
Sign is, 30~40 DEG C of rotating evaporation temperature described in step 2,0.008~0.01Mpa of vacuum degree.
4. the preparation method of the natural sun-prevention moisturizing lipidosome freeze-dried powder of γ-PGA modification according to claim 2, special
Sign is that gamma-polyglutamic acid molecular weight described in step 5 is 70~1,000,000, and gamma-polyglutamic acid concentration of aqueous solution is 0.1%, pH
It is 5~6.
5. the preparation method of the natural sun-prevention moisturizing lipidosome freeze-dried powder of γ-PGA modification according to claim 2, special
Sign is, film whole grain is crossed described in step 5 are as follows: successively by 0.8 μm of filter membrane, 0.65 μm of filter membrane, 0.45 μm of filter membrane and 0.22 μm of filter
Film carries out whole grain.
6. the preparation method of the natural sun-prevention moisturizing lipidosome freeze-dried powder of γ-PGA modification according to claim 2, special
Sign is, freeze thawing treatment process described in step 5 are as follows: freezes 30~60min first in -60 DEG C of environment, is subsequently placed in 15~20
Make its thawing in DEG C environment, is ultrasonically treated 1min after the completion of thawing in 15~20 DEG C of environment, circular treatment 3~4 times.
7. the preparation method of the natural sun-prevention moisturizing lipidosome freeze-dried powder of γ-PGA modification according to claim 2, special
Sign is, freeze-drying process process described in step 5 are as follows: under -60 ± 1 DEG C of environmental condition after pre-freeze 10h, then -52 ± 2
DEG C, vacuum degree less than 100mT environmental condition under be freeze-dried 25h.
8. the natural sun-prevention moisturizing lipidosome freeze-dried powder that the γ-PGA as described in any one of claim 1 is modified is in cosmetics
Using, which is characterized in that then γ-the PGA natural sun-prevention moisturizing lipidosome freeze-dried powder modified is added after water hydratable directly
It is added in cosmetics.
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| CN107982159A (en) * | 2017-11-30 | 2018-05-04 | 宁波秋日小城智能科技有限公司 | A kind of preparation method of longan extract |
| CN108158852A (en) * | 2017-12-29 | 2018-06-15 | 青岛百瑞吉生物工程有限公司 | A kind of sunscreen spray and preparation method thereof |
| CN112656696B (en) * | 2020-12-23 | 2023-01-06 | 杭州优玛达生物科技有限公司 | Composition of molecular motor lipid vesicles and preparation method and application thereof |
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