CN110711152A - Sunscreen cream containing cannabidiol liposome and preparation method thereof - Google Patents
Sunscreen cream containing cannabidiol liposome and preparation method thereof Download PDFInfo
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Abstract
The invention discloses sunscreen cream containing cannabidiol liposome and a preparation method thereof. The sunscreen cream disclosed by the invention comprises the following components in percentage by weight: 0.5-2% of cannabidiol liposome, 0.5-8% of titanium dioxide, 1-8% of diethylamino hydroxybenzoyl hexyl benzoate, 0.5-10% of isoamyl methoxycinnamate, 1-5% of ethylhexyl salicylate, 0.5-1.5% of ethylhexyl palmitate, 2-6% of glyceryl monostearate, 0.5-2.5% of cetostearyl alcohol, 1-10% of glycerol, 1-5% of butanediol, 0.1-1% of allantoin, 0.1-2% of tocopheryl acetate, 0.01-0.5% of xanthan gum, 0.01-0.2% of EDTA-2Na, 0.01-0.1% of preservative and the balance of deionized water. According to the invention, the nano cannabidiol liposome is prepared by adopting a liposome embedding technology, so that the water solubility and stability of Cannabidiol (CBD) are improved, and meanwhile, the transdermal absorption effect of the CBD is effectively promoted, so that the CBD can rapidly penetrate through the surface layer of the skin, directly reach the deep layer of the skin and slowly release the CBD, and the effect is more durable. The sunscreen cream containing the nanoscale cannabidiol liposome has a good sunscreen effect, can effectively remove free radicals and delay skin aging, and the skin feels fresh and not greasy after the sunscreen cream is used.
Description
Technical Field
The invention belongs to the technical field of cosmetics, and particularly relates to sunscreen cream containing cannabidiol liposome and a preparation method thereof.
Background
The skin of human body, especially the face skin, can damage epidermal cells after being excessively exposed to ultraviolet rays, harmful free radicals can be generated after the ultraviolet rays permeate the skin, the free radicals can damage elastic fibers of the skin, the skin is loosened, the aging is accelerated, simultaneously, the increase of the secretion of melanocytes can be stimulated, the synthesis of melanin is increased, and the pigmentation is caused, such as the increase, the enlargement and the deepening of various stains such as black spots, freckles, chloasma and the like; under strong irradiation, the skin can be inflamed and burned, and the skin can be seriously loosened, rough and wrinkled, the blood capillaries can be dilated, and erythema can be generated; it also can reduce skin immunity and cause skin cancer or precancerous lesion. Therefore, the sun protection becomes a necessary link for skin care, and the products are necessary for people to go out.
Cannabidiol is a main active ingredient in cannabinoids, is a non-addictive active ingredient, has no neurotoxicity, and has a series of physiological activity functions of blocking breast cancer metastasis, resisting epilepsy, resisting spasm, resisting rheumatoid arthritis, resisting anxiety, resisting insomnia and the like. Because of its strong antioxidant activity, antibacterial, anti-inflammatory, anti-aging and repairing function to damaged cells, people pay more and more attention to it.
Patent CN109820786A discloses a sunscreen cream for repairing skin injury, which contains cannabis sativa flower and leaf extract, and the main ingredient of the sunscreen cream is cannabidiol oil or cannabidiol Crystal (CBD). However, as the active ingredient cannabidiol belongs to fat-soluble compounds and is insoluble in water, the solubility of the active ingredient can be reduced by directly adding the cannabidiol extract or cannabidiol into the basic formula of the sunscreen cream, so that the active ingredient is not easily absorbed by skin, the bioavailability is greatly reduced, and the effect can not be fully exerted. According to the invention, cannabidiol is prepared into the nanoscale cannabidiol liposome by a liposome embedding technology, so that the water solubility and stability of CBD are improved, the transdermal absorption effect of CBD is effectively promoted, the CBD can quickly penetrate through the surface layer of skin, directly reach the deep layer of skin and slowly release the CBD, and the effect is more durable. The sunscreen cream has good sunscreen effect, can effectively remove free radicals, and has good effects of resisting oxidation and delaying skin aging.
Disclosure of Invention
The invention aims to prepare the nano-scale cannabidiol by using a liposome technology, solve the problem that the cannabidiol is insoluble in water, improve the water solubility and stability of the cannabidiol, and increase the transdermal absorption effect of the cannabidiol, so that CBD can quickly and effectively penetrate through the surface layer of skin to reach the deep layer of the skin and slowly release the CBD, and the effect is obviously and durably. The sunscreen cream disclosed by the invention has a good sunscreen effect, is fresh and cool in skin feel, and can effectively remove free radicals and delay skin aging.
In order to achieve the purpose, the invention adopts the following technical scheme:
1. the sunscreen cream containing cannabidiol liposome is characterized by comprising the following components in percentage by mass: 0.5-2% of cannabidiol liposome, 0.5-8% of titanium dioxide, 1-8% of diethylamino hydroxybenzoyl hexyl benzoate, 0.5-10% of isoamyl methoxycinnamate, 1-5% of ethylhexyl salicylate, 0.5-1.5% of ethylhexyl palmitate, 2-6% of glyceryl monostearate, 0.5-2.5% of cetostearyl alcohol, 1-10% of glycerol, 1-5% of butanediol, 0.1-1% of allantoin, 0.1-2% of tocopheryl acetate, 0.01-0.5% of xanthan gum, 0.01-0.2% of EDTA-2Na, 0.01-0.1% of preservative and the balance of deionized water.
Further, the preservative comprises one or more of cason, nipagin ester, phenoxyethanol and sodium benzoate;
2. the invention provides a preparation method of the sunscreen cream, which comprises the following steps:
(1) preparing an oil phase: placing titanium dioxide, diethylamino hydroxybenzoyl hexyl benzoate, isoamyl methoxycinnamate, ethylhexyl salicylate, ethylhexyl palmitate, glyceryl monostearate, cetearyl alcohol and tocopherol acetate in an oil phase pot according to the proportion, heating to dissolve the titanium dioxide, the diethylamino hydroxybenzoyl hexyl benzoate, the isoamyl methoxycinnamate, the ethylhexyl salicylate, the ethylhexyl palmitate, the glyceryl monostearate, the cetearyl alcohol and the tocopherol acetate, and keeping the temperature at 70-80 ℃.
(2) Preparing an aqueous phase: adding water, glycerol, xanthan gum, butanediol, EDTA-2Na, and allantoin into water phase pot, homogenizing, stirring, heating to 70-80 deg.C, heating to dissolve completely, pumping into emulsifying pot, and maintaining the temperature in the emulsifying pot at 70-80 deg.C.
(3) Emulsification: and (2) adding the oil phase obtained in the step (1) into an emulsifying pot, fully and uniformly stirring with the water phase, and homogenizing and emulsifying at a high speed for 5-10 min.
(4) When the temperature in the emulsifying pot is reduced to 40 ℃, cannabidiol liposome and preservative are added according to the proportion, the mixture is fully and evenly stirred, and the sunscreen cream containing the cannabidiol liposome is obtained after the inspection is qualified and the material is discharged.
3. The invention also provides a preparation method of the nano cannabidiol liposome, which comprises the following steps:
(1) preparing an organic phase: cannabidiol, phospholipid and cholesterol are weighed accurately according to a certain proportion and added into an organic solvent, and the mixture is heated and stirred to be fully dissolved to obtain an organic phase.
(2) Film preparation: evaporating the organic phase obtained in step (1) on a rotary evaporator to remove the solvent, so that the residue forms a phospholipid film on the inner wall of the container.
(3) Hydration: and (3) adding a hydration medium phosphate buffer solution into the container obtained in the step (2), and continuously performing rotary evaporation to enable the phospholipid film to fall off to obtain the cannabidiol liposome suspension.
(4) Homogenizing: and (3) injecting the suspension into a high-pressure homogenizer for homogenizing for 2-3 times, wherein the homogenizing pressure is 30-50 MPa, filtering through a 100-200 nm filter membrane after homogenizing, and extruding.
(5) And (3) drying: and (4) carrying out spray drying on the extrusion liquid to obtain cannabidiol liposome powder.
The invention has the beneficial effects that:
1. according to the invention, the cannabidiol insoluble in water is embedded in the phospholipid bilayer by a liposome embedding technology to form the closed vesicle with hydrophilic outside and lipophilic inside, the particle size of the liposome prepared by the invention reaches the nanometer level, the water solubility and the stability of the cannabidiol can be effectively improved, the cannabidiol is stably encapsulated in the nanoliposome, and the transdermal absorption efficiency and the bioavailability of the cannabidiol can be greatly increased.
2. According to the invention, the nano cannabidiol liposome is added, so that cannabidiol can quickly and effectively penetrate through the surface layer of the skin and directly reach the deep layer of the skin to slowly release cannabidiol, and the effect is obvious and lasting.
3. The sunscreen cream disclosed by the invention has a good sunscreen effect, is fresh and cool in skin feel and not greasy, can effectively remove free radicals, and has good effects of resisting oxidation and delaying senescence.
Detailed Description
The present invention is further illustrated by, but is not limited to, the following examples.
Example 1
(1) The preparation of cannabidiol liposome comprises the following steps:
① preparing organic phase by accurately weighing 10mg cannabidiol, 100mg phospholipid and 20mg cholesterol, adding into ethanol, heating, and stirring to dissolve completely to obtain organic phase.
② film preparation, evaporating the organic phase obtained in step ① on a rotary evaporator to remove the solvent, and forming a phospholipid film on the residue on the inner wall of the container, wherein the rotary evaporation temperature is 40 ℃ and the vacuum degree is 0.1 MPa.
③ hydration, adding 10ml 0.01mol/L phosphate buffer (pH 6.5) slowly into the phospholipid film container, and rotary steaming for 1 hr to remove phospholipid film to obtain cannabidiol liposome suspension.
④ homogenizing, namely injecting the suspension into a high-pressure homogenizer for homogenizing for 3 times, homogenizing under the pressure of 30MPa, filtering through a filter membrane of 200nm, and extruding.
⑤ drying, and spray drying the extruded solution at air inlet temperature of 200 deg.C and air outlet temperature of 90 deg.C to obtain cannabidiol liposome.
(2) A sunscreen cream containing cannabidiol liposome and a preparation method thereof, the steps are as follows:
① oil phase was prepared by placing 1% titanium dioxide, 2% diethylamino hydroxybenzoyl hexyl benzoate, 1% isoamyl methoxycinnamate, 5% ethylhexyl salicylate, 0.5% ethylhexyl palmitate, 2% glyceryl monostearate, 0.5% cetearyl alcohol, 0.5% tocopheryl acetate in a pan containing oil phase, heating to dissolve, and maintaining the temperature at 70 deg.C.
② preparing water phase by adding 50% water, 1% glycerol, 0.05% xanthan gum, 5% butanediol, 0.01% EDTA-2Na, and 0.1% allantoin into water phase pot, homogenizing, stirring, heating to 70 deg.C, heating to dissolve completely, pumping into emulsifying pot, and maintaining the temperature in the emulsifying pot at 70 deg.C.
③ emulsifying, adding the oil phase into an emulsifying pan, stirring with the water phase, and homogenizing at high speed for 5 min.
④ when the temperature in the emulsifying pot is reduced to 40 deg.C, adding cannabidiol liposome 0.5% and cason 0.01% according to the proportion, stirring thoroughly until completely dissolved, adding deionized water to supplement the rest, and discharging after inspection to obtain sunscreen cream containing cannabidiol liposome.
Example 2
(1) The preparation of cannabidiol liposome comprises the following steps:
① preparing organic phase by accurately weighing 10mg cannabidiol, 200mg phospholipid and 50mg cholesterol, adding into ethanol, heating, and stirring to dissolve completely to obtain organic phase.
② film preparation, evaporating the organic phase obtained in step ① on a rotary evaporator to remove the solvent, and forming a phospholipid film on the residue on the inner wall of the container, wherein the rotary evaporation temperature is 40 ℃ and the vacuum degree is 0.2 MPa.
③ hydration, adding 15ml 0.02mol/L phosphate buffer (pH7.0) slowly into the phospholipid film container, and rotary steaming for 2 hr to remove phospholipid film to obtain cannabidiol liposome suspension.
④ homogenizing, namely injecting the suspension into a high-pressure homogenizer for homogenizing for 2 times, homogenizing under 40MPa, filtering with a 200nm filter membrane, and extruding.
⑤ drying, and spray drying the extruded solution at air inlet temperature of 170 deg.C and air outlet temperature of 80 deg.C to obtain cannabidiol liposome.
(2) A sunscreen cream containing cannabidiol liposome and a preparation method thereof, the steps are as follows:
① oil phase was prepared by placing 4% titanium dioxide, 5% diethylamino hydroxybenzoyl hexyl benzoate, 5% isoamyl methoxycinnamate, 3% ethylhexyl salicylate, 1% ethylhexyl palmitate, 4% glyceryl monostearate, 1.5% cetearyl alcohol, 1% tocopheryl acetate in a pan of the oil phase, heating to dissolve it, and maintaining the temperature at 75 ℃.
② preparing water phase by adding 40% water, 5% glycerol, 0.1% xanthan gum, 2% butanediol, 0.1% EDTA-2Na, and 0.5% allantoin into water phase pot, homogenizing, stirring, heating to 75 deg.C, heating to dissolve completely, pumping into emulsifying pot, and maintaining the temperature in the pot at 75 deg.C.
③ emulsifying, adding the oil phase into an emulsifying pan, stirring with the water phase, and homogenizing at high speed for 5 min.
④ when the temperature in the emulsifying pot is reduced to 40 deg.C, adding 1.5% cannabidiol liposome and 0.05% phenoxyethanol, stirring thoroughly until completely dissolved, adding deionized water to supplement the rest part, and discharging after inspection to obtain sunscreen cream containing cannabidiol liposome.
Example 3
(1) The preparation of cannabidiol liposome comprises the following steps:
① preparing organic phase by accurately weighing 10mg cannabidiol, 300mg phospholipid and 50mg cholesterol, adding into ethanol, heating, and stirring to dissolve completely to obtain organic phase.
② film preparation, evaporating the organic phase obtained in step ① on a rotary evaporator to remove the solvent, and forming a phospholipid film on the residue on the inner wall of the container, wherein the rotary evaporation temperature is 40 ℃ and the vacuum degree is 0.3 MPa.
③ hydration, adding 20ml 0.02mol/L phosphate buffer (pH 7.5) slowly into the phospholipid film container, and rotary steaming for 3 hr to remove phospholipid film to obtain cannabidiol liposome suspension.
④ homogenizing, namely injecting the suspension into a high-pressure homogenizer for homogenizing for 2 times, homogenizing under 40MPa, filtering with a 200nm filter membrane, and extruding.
⑤ drying, and spray drying the extruded solution at air inlet temperature of 150 deg.C and air outlet temperature of 70 deg.C to obtain cannabidiol liposome.
(2) A sunscreen cream containing cannabidiol liposome and a preparation method thereof, the steps are as follows:
① oil phase is prepared by placing 8% titanium dioxide, 8% diethylamino hydroxybenzoyl hexyl benzoate, 10% isoamyl methoxycinnamate, 1% ethylhexyl salicylate, 1.5% ethylhexyl palmitate, 6% glyceryl monostearate, 2.5% cetearyl alcohol, 2% tocopheryl acetate in a pan of oil phase, heating to dissolve, and maintaining the temperature at 80 deg.C.
② preparing water phase by adding 40% water, 10% glycerol, 0.5% xanthan gum, 1% butanediol, 0.2% EDTA-2Na, and 1% allantoin into water phase pot, homogenizing, stirring, heating to 80 deg.C, heating to dissolve completely, pumping into emulsifying pot, and maintaining the temperature in the emulsifying pot at 80 deg.C.
③ emulsifying, adding the oil phase into an emulsifying pan, stirring with the water phase, and homogenizing at high speed for 5 min.
④ when the temperature in the emulsifying pot is reduced to 40 deg.C, adding 2% cannabidiol liposome and 0.1% sodium benzoate, stirring thoroughly until completely dissolved, adding deionized water to supplement the rest part, and discharging after inspection to obtain sunscreen cream containing cannabidiol liposome.
Example 4
Evaluation of sunscreen Effect of sunscreen cream
The adhesive tape method comprises the following steps: QB/T2410-1998
Ultraviolet absorbers and ultraviolet screeners in sunscreen cosmetics are capable of absorbing and blocking ultraviolet rays in sunlight. The quartz cell and the medical permeable adhesive tape are used as biological materials for simulating the cuticle and epidermis of a human body, the sunscreen cream is uniformly smeared on the quartz cell and the medical permeable adhesive tape, an ultraviolet spectrophotometer is used as an ultraviolet light source, the ultraviolet absorbance A value of a sample in a UVB area (280 nm-320 nm) is measured, and the sunscreen effect of the product is represented by the absorbance value.
And (3) testing a sample: blank (no sunscreen), control (commercial sunscreen), test (examples 1-3, respectively).
The determination method comprises the following steps:
1. commercially available air permeable medical adhesive tape was cut into a 1cm × 4cm rectangle and adhered to the surface of the quartz cell on the light-transmitting side.
2. Accurately weighing samples (10 +/-0.2 mg), putting on a medical latex finger stall, uniformly smearing the samples, and setting each sample for 3 times.
3. Placing the absorption cell coated with the sample at room temperature (22 + -1 deg.C) for 30min, and measuring absorbance at 280nm-320nm with ultraviolet spectrophotometer.
The results are shown in Table 1.
TABLE 1 evaluation of sunscreen effect of sunscreen cream
| Sample (I) | A280 | A290 | A300 | A310 | A320 | UVB |
| Blank group | 0.458 | 0.416 | 0.373 | 0.321 | 0.214 | 0.356 |
| Is commercially available | 0.719 | 0.686 | 0.618 | 0.574 | 0.517 | 0.623 |
| Example 1 | 0.882 | 0.851 | 0.765 | 0.643 | 0.562 | 0.741 |
| Example 2 | 0.912 | 0.893 | 0.804 | 0.719 | 0.594 | 0.784 |
| Example 3 | 0.854 | 0.796 | 0.811 | 0.695 | 0.531 | 0.737 |
As can be seen from the absorbance values in Table 1, compared with the blank control, the commercial sunscreen cream and the sunscreen cream of the invention have better absorptivity to ultraviolet UVB, and the absorbance values of examples 1 to 3 are slightly higher than those of the commercial products, which shows that the sunscreen cream of the invention has certain sunscreen efficacy.
Example 5
Determination of antioxidant Activity of sunscreen cream
The in vitro antioxidant test proves the effect of the sunscreen cream on eliminating free radicals, and the anti-aging effect of the product is judged according to the theory of free radical aging.
DPPH is a stable free radical, and when a free radical scavenger is added to a DPPH solution, the solution becomes lighter in color, the absorbance at 517nm decreases, and the degree of decrease in absorbance is linear with the degree to which the free radical is scavenged. Therefore, the method can be used for detecting the scavenging condition of free radicals so as to evaluate the antioxidant capacity of a certain substance, and the capacity is expressed by the Scavenging Rate (SR), and the larger the scavenging rate is, the stronger the antioxidant capacity is.
Test materials: examples 1-3, commercial sunscreens;
the experimental method comprises the following steps: dissolving 1g of the sunscreen cream in 2mL of absolute ethanol, adding 2mL of 60 mu mol/L DPPH solution after complete dissolution, uniformly mixing, standing for 30min, adjusting to zero by using absolute ethanol, and measuring the absorbance at 517nm to be Ai; in the same method, 2mL of absolute ethyl alcohol solvent and 2mL of DPPH solution are uniformly mixed, and the absorbance is determined to be Ac; and dissolving 1g of sunscreen cream in 2mL of absolute ethyl alcohol, adding 2mL of absolute ethyl alcohol solvent after complete dissolution, uniformly mixing, and determining the absorbance Aj. The radical scavenging rate was calculated by the following formula.
The clearance (%) [1- (Ai-Aj)/Ac ] x 100%
Wherein Aj reflects the contribution of the sample itself to absorbance; absorbance values of Ai samples after action on DPPH; ac is the absorption of DPPH itself at the measurement wavelength.
The results of the experiment are shown in table 2:
TABLE 2 sunscreen cream free radical (DPPH) clearance
| Sample (I) | Clearance (%) |
| Example 1 | 90.5 |
| Example 2 | 92.3 |
| Example 3 | 91.7 |
| Is commercially available | 82.9 |
The results in Table 2 show that compared with the commercial sunscreen cream, the sunscreen cream provided by the invention has better capability of scavenging free radicals, and the antioxidant effect is better.
Example 6
Skin feel testing of sunscreen
To test the skin feel of the sunscreen of the present invention, 30 volunteers with oily or mixed skin, aged 20-35 years, were tried with the sunscreens described in examples 1-3, respectively. The use method comprises the following steps: a proper amount of sunscreen cream is uniformly smeared on the skin at the back of the cleaned face, and the trial effect is shown in table 3.
Table 3 skin feel test results for examples 1-3
| Index (I) | Good effect | In general | Is poor |
| Degree of freshness | 27 | 3 | 0 |
| Absorbency of | 26 | 3 | 1 |
| Spreadability | 23 | 5 | 2 |
And (4) evaluating the results: the sunscreen cream disclosed by the invention is fresh and cool in skin feel, not greasy and good in absorption effect after being used.
The above description is only an exemplary embodiment of the present invention, and is not intended to limit the scope of the present invention. It will be apparent to those skilled in the art that equivalent embodiments or modifications without departing from the technical spirit of the present invention are within the scope of the present invention.
Claims (9)
1. The sunscreen cream containing cannabidiol liposome is characterized by comprising the following components in percentage by mass: 0.5-2% of cannabidiol liposome, 0.5-8% of titanium dioxide, 1-8% of diethylamino hydroxybenzoyl hexyl benzoate, 0.5-10% of isoamyl methoxycinnamate, 1-5% of ethylhexyl salicylate, 0.5-1.5% of ethylhexyl palmitate, 2-6% of glyceryl monostearate, 0.5-2.5% of cetostearyl alcohol, 1-10% of glycerol, 1-5% of butanediol, 0.1-1% of allantoin, 0.1-2% of tocopheryl acetate, 0.01-0.5% of xanthan gum, 0.01-0.2% of EDTA-2Na, 0.01-0.1% of preservative and the balance of deionized water.
2. The sunscreen cream containing cannabidiol liposomes as claimed in claim 1 and the preparation method thereof, wherein the preparation process of the sunscreen cream is as follows:
(1) preparing an oil phase: placing titanium dioxide, diethylamino hydroxybenzoyl hexyl benzoate, isoamyl methoxycinnamate, ethylhexyl salicylate, ethylhexyl palmitate, glyceryl monostearate, cetearyl alcohol and tocopherol acetate in an oil phase pot according to the proportion, heating to dissolve the titanium dioxide, the diethylamino hydroxybenzoyl hexyl benzoate, the isoamyl methoxycinnamate, the ethylhexyl salicylate, the ethylhexyl palmitate, the glyceryl monostearate, the cetearyl alcohol and the tocopherol acetate, and keeping the temperature at 70-80 ℃;
(2) preparing an aqueous phase: adding water, glycerol, xanthan gum, butanediol, EDTA-2Na, and allantoin into water phase pot, homogenizing, stirring, heating to 70-80 deg.C, heating to dissolve completely, pumping into emulsifying pot, and maintaining the temperature in the emulsifying pot at 70-80 deg.C;
(3) emulsification: adding the oil phase obtained in the step (1) into an emulsifying pot, fully and uniformly stirring with the water phase, and homogenizing and emulsifying at a high speed for 5-10 min;
(4) when the temperature in the emulsifying pot is reduced to 40 ℃, cannabidiol liposome and preservative are added according to the proportion, the mixture is fully and evenly stirred, and the sunscreen cream containing the cannabidiol liposome is obtained after the inspection is qualified and the material is discharged.
3. The sunscreen cream containing cannabidiol liposomes as claimed in claim 1 or 2, and the preparation method thereof, wherein the preparation method of cannabidiol liposomes comprises the following steps:
(1) preparing an organic phase: accurately weighing cannabidiol, phospholipid and cholesterol according to a certain proportion, adding into an organic solvent, heating and stirring to fully dissolve the cannabidiol, the phospholipid and the cholesterol to obtain an organic phase;
(2) film preparation: evaporating the organic phase obtained in the step (1) on a rotary evaporator to remove the solvent, so that the residue forms a uniform film on the inner wall of the container;
(3) hydration: adding a hydration medium phosphate buffer solution into the container obtained in the step (2), and continuously performing rotary evaporation to enable a phospholipid film to fall off to obtain a cannabidiol liposome suspension;
(4) homogenizing: injecting the suspension into a high-pressure homogenizer for homogenization, and filtering and extruding through an extruder after homogenization;
(5) and (3) drying: and (4) carrying out spray drying on the extrusion liquid to obtain cannabidiol liposome powder.
4. The sunscreen cream containing cannabidiol liposomes as claimed in claim 1 or 2, and the preparation method thereof, wherein the preservative comprises one or more of cason, paraben, phenoxyethanol, and sodium benzoate.
5. The method of claim 3, wherein the phospholipid comprises any one of egg yolk lecithin, hydrogenated egg yolk lecithin, soybean lecithin, hydrogenated soybean lecithin, sphingomyelin, phosphatidylethanolamine, dimyristoylphosphatidylcholine (i.e., DMPC), dimyristoylphosphatidylglycerol (i.e., DMPG), dipalmitoylphosphatidylcholine, distearoylphosphatidylcholine, dioleoylphosphatidylcholine, dilauroylphosphatidylcholine, and combinations thereof; the organic solvent comprises methanol, ethanol, dichloromethane, chloroform and their mixture.
6. The method for preparing cannabidiol nanoliposomes according to claim 3, wherein the ratio of phospholipid to cholesterol is 2-6: 1, cannabidiol to phospholipid ratio of 1: 10 to 30.
7. The method for preparing cannabidiol nanoliposomes according to claim 3, wherein the rotary evaporation temperature is 40-60 ℃, the vacuum degree is 0.1-0.6 MPa, and the rotary evaporation time is 1-3 h.
8. The method for preparing cannabidiol nanoliposomes according to claim 3, wherein the phosphate buffer solution has a pH of 6-8 and a concentration of 0.01-0.05 mol/L.
9. The method for preparing cannabidiol nanoliposome as claimed in claim 3, wherein homogenizing is performed for 2-3 times, the homogenizing pressure is 20-50 MPa, and the pore diameter of the filter membrane is 100-200 nm; spray drying conditions: the air inlet temperature is 150-200 ℃, and the air outlet temperature is 70-110 ℃.
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| Application Number | Priority Date | Filing Date | Title |
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| CN201911060504.1A CN110711152A (en) | 2019-11-01 | 2019-11-01 | Sunscreen cream containing cannabidiol liposome and preparation method thereof |
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| CN201911060504.1A CN110711152A (en) | 2019-11-01 | 2019-11-01 | Sunscreen cream containing cannabidiol liposome and preparation method thereof |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN114601743A (en) * | 2022-03-22 | 2022-06-10 | 宝萃生物科技有限公司 | Dihydromyricetin liposome and preparation and application thereof |
| CN115350099A (en) * | 2022-08-26 | 2022-11-18 | 广州栋方生物科技股份有限公司 | Emulsification method and application of oil-in-water type sunscreen composition |
-
2019
- 2019-11-01 CN CN201911060504.1A patent/CN110711152A/en not_active Withdrawn
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN114601743A (en) * | 2022-03-22 | 2022-06-10 | 宝萃生物科技有限公司 | Dihydromyricetin liposome and preparation and application thereof |
| CN114601743B (en) * | 2022-03-22 | 2022-09-13 | 宝萃生物科技有限公司 | Dihydromyricetin liposome and preparation and application thereof |
| CN115350099A (en) * | 2022-08-26 | 2022-11-18 | 广州栋方生物科技股份有限公司 | Emulsification method and application of oil-in-water type sunscreen composition |
| CN115350099B (en) * | 2022-08-26 | 2023-12-29 | 广州栋方生物科技股份有限公司 | Emulsification method of oil-in-oil type sun-screening composition and application thereof |
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