KR20190085686A - Cosmetic composition comprising micro algae extracts stabilized in algaesome as active ingredient - Google Patents

Abstract

The present invention relates to a cosmetic composition for preventing skin aging, containing Algasome comprising a microalgae extract therein. Algasome of the present invention comprises a microalgae extract captured in a lipid complex comprising phospholipid, phytosphingosine, ceramide 3, cholesterol, phytosterol, beta-sitosterol and squalene, thereby having an excellent skin-delivery-effect. Therefore, the cosmetic composition containing Algasome comprising a microalgae extract, according to the present invention, exhibits excellent skin wrinkle reduction, skin elasticity improvement and moisturization effects.

Description

[0001] The present invention relates to a cosmetic composition comprising algae containing microalgae extract,

The present invention relates to a cosmetic composition containing an algae containing a microalgae extract.

Before aging of skin, the skin cells are active physiologically. In other words, in the fibroblast in the dermis, synthesis and metabolism of collagen important for suppressing the elasticity and wrinkle formation of the skin are active, and also, it is necessary for the skin including the hyaluronic acid GAGS (Glucosaminoglycans), a protein, is also actively synthesized, which can impart elasticity, softness, flexibility and moisturizing properties to the skin. In epidermis, proliferation of basal layer cells is active, and the production of binding proteins such as laminin, integrin, and desmosomes that enhance binding between skin cells is balanced Strengthens skin tissue to maintain healthy skin.

However, contemporary human skin is exposed to various harmful environments and stresses, and various kinds of damage are being experienced. As the age of the skin lowers the physiological activity of the skin and the recovery speed of the damaged skin is slowed down, the skin is deteriorated in elasticity, There are various skin aging phenomena such as calmness, black spots, and dullness. Therefore, the biggest goal of cosmetics is to improve the symptoms of aging, and a variety of cosmetics are being developed for this purpose.

In general, skin aging phenomena such as skin elasticity reduction, skin wrinkles and pigmentation are caused by collagen breakdown, reduction of collagen synthesis, disappearance of elastin, and melanin pigmentation due to lowering of skin physiology. It is a phenomenon. In recent years, many skin physiological studies have been conducted to prevent skin aging. Actually, ultraviolet ray blocking agents, antioxidants, cell activation promoters and the like have been used to prevent skin wrinkles in cosmetics and methods for improving skin wrinkles due to collagen synthesis have been proposed .

On the other hand, based on the advances in biochemistry and molecular biology, a small amount of signal substances such as growth factors existing in the living body have been found, and the aging theory of living bodies based on them has been reestablished. In addition, this signal substance decreases in vivo with increasing age. It has been found that the decrease of this growth factor is closely related to our aging.

Especially, reactive oxygen radicals and free radicals, which are generated due to increase of ultraviolet ray amount, air pollution and excessive fatigue and stress of modern people, oxidize or denature biological components (protein, nucleic acid, cell membrane lipid, etc.) As the main cause of the rise, interest in free radicals is increasing. However, as the age increases, the biosynthesis of various biologic antioxidants decreases, and oxidative stress due to various radicals is exposed to the harmful environment such as pollution of the atmospheric environment, unhealthy diet, ultraviolet ray exposure, stress and disease It becomes difficult to erase sufficiently, and when exposed to such oxidative stress, the skin ages and changes variously such as wrinkles, decreased elasticity, stain, freckles, and drying.

In order to solve the problem of skin aging, U.S. Patent Nos. 4603146 and 4877805 disclose examples using retinoids (vitamin A). However, retinol is very limited in the cosmetic field due to the problem that it is discolored, detached, and titered because it is prescribed to the product and its effect is decreased and skin irritation is caused despite the use of a small amount.

Therefore, various studies for stabilizing such retinoids are under way. For example, U.S. Patent No. 3,906,108 discloses an oil-in-water (O / W) emulsifier type in which retinoic acid is stabilized by using BHT, dl-α-tocopherol as an oil-oxidizing agent and EDTA as a chelating agent, and U.S. Patent No. 4,247,547 US Pat. No. 4,826,828 discloses a method for producing retinol, which comprises retinol, retinyl acetate, and retinyl palmitate using BHT and BHA, which are oil-soluble oxidizing agents, in order to stabilize retinol by using citric acid and tocopherol, And United States Patent 4,720,353 discloses a water-in-oil type in which retinol is stabilized by using antioxidants such as BHA, ascorbic acid and tocopheryl linoleate. In EP 0440398 BI, an oil-in-water type emulsion composition containing at least one water-soluble antioxidant, at least one oil-soluble antioxidant, and a chelating gel is disclosed in order to improve the chemical stability of retinoids. European Patent No. 1568106A1 discloses a retinoid A water-soluble antioxidant, an oil-in-water type emulsion composition containing an antioxidant and an free base type imidazole simultaneously present in an oil phase and an aqueous phase of an emulsion, and a chelating agent and an oil-soluble antioxidant.

Thus, substances having excellent effects for improving wrinkle, moisturizing and elasticity have problems in safety and stability, while those having excellent safety and stability are relatively weak in clinical efficacy, And a prolonged period of time.

Liposomes are widely used not only in the pharmaceutical field but also in cosmetics and foods. This has the advantage that the physiologically active ingredient can be stably delivered without breakage, and that the physiologically active ingredient can be both lipid-soluble and water-soluble. Liposomes are divided into multilamellar vesicles (MLV) and unilamellar vesicles (ULV). ULV can be divided into Large Unilamella vesicles and Small Unilamella vesicles. The size of MLV is 400 ~ 3,500nm, the content of liposome is 5 ~ 15%, the size of LUV is 100 ~ 1,000nm, the content of liposome is 36 ~ 65% Is 20 to 50 nm and the amount that can be contained in the liposome is 0.5 to 1.0%. The amount of physiologically active substance that can be contained is the most LUV but unstable, the SLV is the most stable, but the amount that can be contained is small.

A cell membrane with a bilayer structure in the form of a multilamellar vesicle (MLV) is a constituent of all cells and distinguishes the inside and the outside of the cell. The cell membrane is a thin, structured phospholipid bilayer composed of phospholipids and protein molecules, and has selective permeability. Cell surface membranes also have receptor proteins and cell adhesion proteins (membrane proteins). There are other proteins that perform other functions, and these proteins play a very important role in maintaining constant cell function and organization at all times. In animal cells, cell membranes perform this role alone, whereas in yeast, bacteria, and plants, cell walls form the outermost boundaries and provide physical protection. The cell membrane is approximately 10 nanometers thick and can be faintly separated by transmission electron microscopy. Another important role of cell membranes is to maintain cell potential.

The basic structure and structure of cell membranes are the same as those of membranes surrounding other cell organelles. The cell membrane is composed of two layers of phospholipid, a phospholipid bilayer, and the cell membrane is described as a flow mosaic structure. A flow mosaic is a two-dimensional flow in which lipids float freely and has a protein acting as a channel or receptor across the cell membrane. This cell membrane model was developed in 1971 by S.J. Singer proposed a lipid protein model, and in 1972 G.L. Nicolson has attached carbohydrates to present its flow properties. Cells vary in the type and amount of lipids to maintain cell membrane fluidity. Cholesterol molecules (in the case of eukaryotic cells) or hopanoids (in the case of prokaryotes) inside the bilayer help to maintain fluidity. In fact, all lipid molecules inside the cell membrane are not fluids in the sense that they can diffuse freely. That is, Caveola is relatively fixed in the cell membrane region, and many proteins do not diffuse. The cytoskeleton supports the cell membrane, immobilizes the intracellular proteins, directs them to a specific surface, and also limits the extent to which proteins can move within the cell membrane. The cell membrane surface always shows some structure, not a fluid that moves without form. Synapse is an example of a more structural cell membrane.

The new material is contained in or removed from the cell membrane in a number of ways: For example, the fusion of intercellular vesicles not only secretes vesicle internals, but also includes the vesicle membrane material into the cell membrane, which in turn forms vesicles that eventually become vesicles. In another example, if the cell membrane has a tubular structure, the material from the tube can be absorbed into the cell membrane. As another example, molecular exchange is possible in these aqueous materials, even if the concentration of aqueous material in the cell membrane is low (stable cell membrane constituents are not well soluble in water). In all of the above cases, the cell membrane tension affects the exchange rate of the material. In some cells, particularly in cells with a smooth shape, the cell membrane tension and area are related to elasticity and fluidity, respectively. The association with time is called homeostasis, which is how much the area or tension is constant over time.

MLV, which has a multilamellar structure similar to the cell membrane structure of the skin, has an affinity for the skin but is too large to penetrate the skin.

Korean Patent No. 10-0750870 discloses a method of extracting seaweed extracts from Salina species of the genus Dunaliella with Dunaliellaceae of Chlamydomonadales neck of Chlorophyceae And a cosmetic composition for improving skin transparency.

The inventors of the present invention found that a microalgae extract can be effectively used as an active ingredient of a skin anti-aging cosmetic composition in accordance with this purpose while seeking a substance for improving skin aging, and completed the present invention.

Conventional liposome manufacturing processes have suffered from heat stability of various physiologically active substances in microalgae extract by utilizing a high-pressure microfluidizer generating heat of 60 ° C or more. The present invention complements the disadvantages of conventional MLV liposomes which have disadvantages when using the above-described high-pressure emulsification device and have skin and affinity but are too large to penetrate the skin, thereby assuring skin-friendly and physiologically active ingredients as much as possible (Algaesome), which is easy to manufacture as well as being able to make microalgae extracts at room temperature to stabilize and penetrate into the skin.

It is an object of the present invention to provide a cosmetic composition excellent in anti-aging effect of skin containing alginate stabilized by containing microalgae extract.

The present invention relates to a cosmetic composition for preventing skin aging and containing an algae containing a microalgae extract in a lipid complex.

Preferably, the algae contain a microalgae extract in a lipid complex consisting of phospholipid, phytosphingosine, ceramide 3, cholesterol, phytosterol, beta-sitosterol and squalene.

Preferably, the algae comprises 0.01-30.0% by weight phospholipid, 0.01-10.0% by weight of phytosphingosine, 0.01-50.0% by weight of ceramide 3, 0.01-30.0% by weight of cholesterol, 0.01-0.01% by weight of phytosterol 0.01 -20.0% by weight, beta-sitosterol 0.01-10.0% by weight, squalene 0.01-80.0% by weight and microalgae extract 0.01-50.0% by weight.

Preferably, the cosmetic composition for anti-aging has at least one of an effect of improving skin wrinkles, a skin elasticity improving effect, and a skin moisturizing effect.

Preferably, the algae are contained in an amount of 0.01 to 100.0% by weight based on the total weight of the entire cosmetic composition.

Preferably, the above-mentioned algosa is dissolved at high pressure to form a lipid complex in the form of a translucent gel, and the lipid complex is formed at room temperature. The lipid complex may be prepared by dissolving phosphatidylserine, phytosphingosine, ceramide 3, cholesterol, phytosterol, beta-sitosterol, And a microalgae extract.

Preferably, the microalgae extract is cultured with Dunaliella Salina .

Preferably, the cosmetic composition is selected from the group consisting of solutions, suspensions, emulsions, pastes, gels, creams, lotions, powders, soaps, surfactant-containing cleansing, oils, powder foundations, emulsion foundations, wax foundations and sprays And may have a formulation to be selected.

The cosmetic of the present invention can contain a microalgae extract in an algae and stabilize it, so that the skin transfer effect is superior to that of a conventional liposome-capturing cosmetic, and thus, a better anti-aging effect of the skin can be obtained. In addition, since the active ingredient of the microalgae extract is stabilized at the alum size at room temperature, destruction of the active ingredient can be prevented.

FIG. 1 shows particle distributions of algae containing microalgae extract prepared in Example 1 according to the present invention.
FIG. 2 is a polarized microscope photograph of algae containing the microalgae extract prepared in Example 1. FIG.

Unless defined otherwise, all technical terms used in the present invention have the following definitions and are consistent with the meaning as commonly understood by one of ordinary skill in the art to which this invention pertains. In addition, preferred methods or samples are described in this specification, but similar or equivalent ones are also included in the scope of the present invention.

The term "about" is used herein to refer to a reference amount, level, value, number, frequency, percentage, dimension, size, quantity, weight or length of 30, 25, 20, 15, 10, 9, 8, 7, Level, value, number, frequency, percent, dimension, size, quantity, weight or length of a variable, such as 4, 3, 2 or 1%.

Throughout this specification, the words "comprises" and "comprising ", unless the context requires otherwise, include the steps or components, or groups of steps or elements, Steps, or groups of elements are not excluded.

The present invention relates to a skin anti-aging cosmetic composition containing algaesome containing microalgae extract which improves the skin aging phenomenon.

In the present invention, prevention of skin aging is used in a broad sense to include wrinkle improvement, elasticity enhancement effect, skin moisturizing effect and skin regeneration effect.

In the present invention, the microalgae extract includes all the extracts obtained by culturing microalgae.

For example, the microalgae extract may be extracted with one or more extraction solvents selected from the group consisting of various extraction solvents available in the art, for example, purified water, alcohol, butylene glycol, glycerin, acetate, , Preferably ethanol or purified water, and more preferably can be extracted using purified water.

In addition, the microalgae extract according to the present invention includes an ultrasonic extract. Ultrasonic extraction may be carried out by extracting microalgae in an extraction solvent, for example, an extraction solvent selected from the group consisting of purified water, alcohol, butylene glycol, glycerin, acetate, hexane and the like, followed by extraction with an ultrasonic extractor.

As used herein, the term "microalgae " refers to a single-celled plant, often referred to as phytoplankton, which is only observable by microalgal microscopy and has no roots, stems, or leaves. In addition, blue-green, yellow, red, and brown, depending on the pigment, are typical microalgae such as diatoms and dinoflagellates.

In the microalgae culturing method of the present invention, a closed photobioreactor system capable of producing an expensive physiologically active substance and culturing at a high concentration was used. The photobioreactor used in the present invention maximizes the surface area per unit volume to efficiently transmit light, selects an effective light source that can increase the utilization efficiency of the total light energy, and transmits light efficiently from the light source to the cell. Thereby maximizing the concentration.

The term "algae" in the present invention means a case where a vesicle containing a microalgae extract is captured and stabilized.

In order to produce a skin-friendly and stable Vesicle, the alges according to the present invention can be used as a main lipid component constituting the cell membrane such as phospholipid, phytosphingosine, ceramide 3, cholesterol Dissolving cholesterol, phytosterol, beta-sitosterol and squalene at high pressure to prepare a lipid complex in the form of a translucent gel; and mixing the lipid complex and the microalgae extract at room temperature . ≪ / RTI >

Specifically, the main lipid components constituting the cell membrane are phospholipid, phytosphingosine, ceramide 3, cholesterol, phytosterol, β-sitosterol ) And squalene are dissolved at high pressure using a high-pressure emulsifier (Microfludizer) to make a translucent gel-like lipid complex. Next, an appropriate amount of the lipid complex is added to an aqueous solution in which the microalgae extract is dispersed at room temperature to obtain an algae containing the microalgae extract of the present invention having a closed double-layer structure. At this time, the temperature of the reaction process proceeds at room temperature. The conventional liposome manufacturing process using a high-pressure microfludizer has a disadvantage in that heat stability of temperature-sensitive components can not be secured due to the formation of liposomes at a high temperature (60 ° C or higher). The present invention differs from the use of a high pressure emulsification apparatus for mixing liposome composites, but the use of a high pressure emulsification apparatus for forming liposomes of the prior art. In the present invention, a lipid complex dissolved and mixed in a high-pressure emulsification apparatus is formed, and they form a vesicle in an aqueous solution phase and form an algae by inlaying an effective ingredient, i.e., a microalgae extract, dispersed in an aqueous solution .

In order to obtain the desired effect of the present invention, the content of the microalgae extract may be 0.001 to 50.0% by weight, preferably 0.1 to 20.0% by weight based on the total weight of the algae.

The phospholipid constituting the algae is contained in an amount of 0.001-30.0% by weight, preferably 0.1-10.0% by weight, based on the total weight, based on the total weight of the algae. Phytosphingosine is contained in an amount of 0.01-10.0% by weight, preferably 0.1-5.0% by weight, based on the total weight of the algae. Ceramide 3 is contained in an amount of 0.01 to 50.0% by weight, preferably 0.1 to 30.0% by weight based on the total weight of algae. Cholesterol is contained in an amount of 0.01-30.0% by weight, preferably 0.01-20.0% by weight, based on the total weight of the algae. Phytosterol is contained in an amount of 0.01-20.0% by weight, preferably 0.1-10.0% by weight, based on the total weight of the algae. The beta-sitosterol is contained in an amount of 0.01-10.0% by weight based on the total weight of the algae, preferably 0.1-5.0% by weight based on the total weight. Squalene content is 0.01-80.0 wt% based on the total weight of algae, preferably 0.1-50.0 wt% based on the total weight.

The average droplet size of the algae thus prepared is 20 to 500 nm, preferably 50 to 200 nm, and most preferably 80 to 150 nm. When the average droplet size of the above algae exceeds 500 nm, skin penetration and formulation stability are very weak.

In order to obtain the desired effects of the present invention, ALGAESOME may be contained in an amount of 0.01 to 100.0% by weight, preferably 0.1 to 20.0% by weight based on the total weight of the cosmetic composition.

The components contained in the cosmetic composition of the present invention may contain, in addition to the microalgae extract, various components which maintain the activity of the biological component, components commonly used in cosmetic compositions such as antioxidants, stabilizers, solubilizers, vitamins , Customary adjuvants such as pigments and flavoring agents, and carriers, and the like.

The cosmetic composition containing the alginate containing the microalgae extract of the present invention is not particularly limited in its formulation and may be, for example, softening lotion, astringent lotion, nutritional lotion, eye cream, serum, It can be applied to the formulations of massage cream, cleansing cream, cleansing lotion, cleansing foam, cleansing water, body cleanser, body lotion, body cream, powder, essence, pack, hair tonic, hair treatment, shampoo, rinse, Lt; / RTI > In addition, in the cosmetic composition of each formulation, the other ingredients for further enhancing the efficacy in addition to the algae containing the microalgae extract may be selected and mixed without difficulty by those skilled in the art depending on the formulation or use purpose of other cosmetics.

Hereinafter, the constitution and effects of the present invention will be described in detail with reference to Examples, Experimental Examples, Production Examples and Formulation Examples. However, it should be understood that these examples, experimental examples, preparation examples and formulation examples are provided only to illustrate the present invention to those skilled in the art, And the formulation examples are not intended to be limiting, and the examples, experimental examples, preparation examples and formulation examples according to the present invention may be modified into various forms.

[ Manufacturing example  1] Preparation of microalgae extract

The microalgae used for the present invention were Dunaliella Salina from KMMCC (Korea Marine Microalgae Bank), and the medium used for culture was F / 2 medium (Guillard and Ryther 1962) And sterilized at 121 占 폚 for 15 minutes. For the present invention, Dunaliella < RTI ID = 0.0 > Salina ) was inoculated into a closed photobioreactor system, the culture temperature was adjusted to 22 ° C, and a white LED having a wavelength of 440 nm was constantly supplied at 60 W intensity for 10 days to obtain microalgae of the present invention. The cultured microalgae were centrifuged at 10,000 rpm for 10 minutes, and the supernatant containing the medium was discarded. Only the microalgae were separated, followed by centrifugation at 10,000 rpm for 10 minutes by adding purified water. The supernatant was discarded, The microalgae of the present invention are obtained. Add purified water to the obtained microalgae and sonicate (1min on, 30seconds off, 22.5kHz) 10 times. After sonication, centrifuge at 10,000 rpm for 10 minutes to separate the supernatant except for the precipitate, and centrifuge again at 10,000 rpm for 5 minutes. The supernatant thus obtained was filtered with a 6 μm filter to prepare microalgae extract of the present invention.

[ Manufacturing example  2] Containing microalgae extract ALGERSOME (ALGAESOME)  Produce

Algaesome has the same contents as in Table 1 below, such as phospholipid, phytosphingosine, ceramide 3, cholesterol, phytosterol, beta-sitosterol β-sitosterol and squalene were passed through a high-pressure microfludizer three times at a pressure of 1,000 bar to form a translucent gel-like lipid complex. A solution in which a microalgae extract (Preparation Example 1) was dispersed on an aqueous solution was prepared. Next, at the room temperature, the lipid complex was added to the aqueous phase containing the microalgae extract to prepare the microalgae extract - captured algae.

ingredient weight% Phospholipid
Phytosphingosine
Ceramide 3 (Ceramide 3)
Cholesterol
Phytosterol
Beta-sitosterol < / RTI >
Squalene
Microalgae extract (Preparation Example 1) 5.0
2.5
25.0
2.5
2.5
2.5
50.0
10 system 100

[ Experimental Example  One] Alge  Particle size distribution

The following experiment was conducted to examine the particle size distribution of the algae particles containing the microalgae extract of the present invention. The algae of Preparation Example 2 in Table 1 was diluted to a suitable concentration in 10% -Microplasma Hyopnemoniae OD410 = 0.1 buffer solution, and the particle size of the algae particles was measured. The particle size of the alumina particles was analyzed by using a Zetasizer NS apparatus and the results are shown in the graph of FIG. The average particle size of the obtained algae is 120 nm, which is smaller than that of the liposome prepared using the conventional high pressure emulsifier (microfluidizer), and the homogeneity of the particle size can be improved and the stability can be improved. FIG. 2 shows an SEM photograph of the algae (Preparation Example 2) containing the microalgae extract prepared according to the present invention.

[ Example  1] Containing microalgae extract To know  Containing Cosmetics  Preparation of composition

As shown in Table 2, a composition (Example 1) containing the alginate containing the microalgae extract prepared in Preparation Example 2 and a composition containing the existing liposome containing the microalgae extract (Comparative Example 1) were prepared. The conventional liposome used in Comparative Example 1 (Comparative Preparation Example 1) was prepared by mixing 3.0 wt% of phospholipid, 10.0 wt% of propylene glycol, 10.0 wt% of ethyl alcohol, 3.0 wt% of mineral oil and 64 wt% of purified water, , And then 10.0% by weight of the microalgae extract was poured into the above-mentioned solution, stirred, and then passed through a high pressure microfludizer at a pressure of 1,000 bar twice.

(Unit: wt%)) ingredient Example 1 Comparative Example 1 Totally 0.2 0.2 Algussa (Preparation Example 2) 10.0 - Liposome (Comparative Preparation Example 1) - 10.0 A Arachidyl alcohol 3.0 3.0 Glyceryl stearate 1.5 1.5 Squalane 5.0 5.0 Hydroxypolydecin 2.0 2.0 Trioctanoin 5.0 5.0 Polysorbate 80 1.0 1.0 Sorbitan stearate 0.5 0.5 Cyclopentasiloxane 3.0 3.0 Tocopheryl acetate 0.2 0.2 BHT 0.05 0.05 B Purified water Balance Balance Concentrated glycerin 4.0 4.0 1,3-butylene glycol 2.0 2.0 Dimethicone / Vinyl Dimethicone Crosspolymer 0.4 0.4 EDTA-2Na 0.05 0.05 Incense, preservative Suitable amount Suitable amount system 100 100

[ Experimental Example  2] Effect of improving skin wrinkles ( Instrumental  evaluation)

The skin wrinkle improving effect (mechanical evaluation) of the cosmetic containing the alginate containing the microalgae extract of the present invention was measured.

Twenty women aged 20 or older (mean age 35.2 years) were divided into two groups (A and B). In Group A, Example 1 was compared to Group B, and Comparative Example 1 was divided into 2X2 cm2 (SKIN VISIOMETER SV400, C + K Electronics GmbH, Germany) with replica of skin wrinkle using a transparent silicone solution while applying for 8 weeks (twice / day, 0.2g / Changes in skin wrinkles were measured. The replica image is three-dimensionally analyzed with a CCD camera, and the average roughness roughness (R _z ) of the following formula (1), which is a value obtained by dividing the sum of roughnesses of respective wrinkles (R _m : m is an integer of 1 or more) The wrinkle improvement effect was analyzed. Test results for the effect of improving skin wrinkles after 6 weeks are shown in Table 3 below.

[Equation 1]

Average roughness roughness (R _z, μm) Example 1 Comparative Example 1 T0 T8 ΔR _z T0 T8 ΔR _z Subject 1 129 62 -67 128 86 -42 Subject 2 128 41 -87 130 78 -52 Subject 3 121 38 -83 124 82 -42 Subject 4 132 55 -77 135 94 -41 Subject 5 120 22 -98 115 46 -69 Subject 6 136 43 -93 130 75 -55 Subject 7 128 36 -92 125 78 -47 Subject 8 121 39 -82 118 69 -49 Subject 9 129 43 -86 122 91 -31 Subject 10 125 41 -84 117 78 -39 Average -84.9 -46.7

As can be seen from the test results in Table 3, the average wrinkle roughness (R _z ) after 8 weeks was 84.9 쨉 m lower for cosmetics containing algae containing microalgae extract (Example 1) (p < 0.01) The wrinkles were improved by about 81.8% as compared with Comparative Example 1 containing the ordinary liposome containing microalgae extract. From the above results, it can be seen that Example 1 containing algae containing microalgae extract showed a more effective wrinkle-reducing effect than Comparative Example 1 containing a general liposome stabilized with microalgae extract.

[ Experimental Example  3] Skin wrinkle improvement effect (visual evaluation)

Visual evaluation of the improvement of skin wrinkles on cosmetics containing algae containing the microalgae extract of the present invention was carried out.

Twenty women aged 20 or older (mean age 35.2 years) were divided into two groups (A and B). Group A was compared with Example 1 and Group B was compared with Comparative Example 1 After the application for 8 weeks (twice / day), the degree of improvement (? W) of the skin wrinkles was measured by classifying the subjective evaluation of the expert and the subjective evaluation of the subject into the following six grades. Clinical effects of skin wrinkle improvement are shown in Table 4 (objective evaluation of expert) and Table 5 (subjective evaluation of subject).

Evaluation of skin wrinkles Evaluation criteria:

-3: Extremely worse -2: worse -1: slightly worse

0: No change 1: Slight improvement 2: Improvement 3: Highly improved

Clinical effect of skin wrinkle improvement (objective evaluation of expert) Skin wrinkle improvement (ΔW) Example 1 Comparative Example 1 T0 T8 T0 T8 Subject 1 0 3 0 2 Subject 2 0 3 0 One Subject 3 0 3 0 2 Subject 4 0 2 0 One Subject 5 0 2 0 2 Subject 6 0 3 0 2 Subject 7 0 3 0 One Subject 8 0 3 0 3 Subject 9 0 2 0 2 Subject 10 0 3 0 2 DELTA W (T8-T0) 2.7 1.8

Clinical effect of skin wrinkle improvement (subject's subjective evaluation) Skin wrinkle improvement (ΔW) Example 1 Comparative Example 1 T0 T8 T0 T8 Subject 1 0 3 0 2 Subject 2 0 2 0 3 Subject 3 0 3 0 One Subject 4 0 2 0 2 Subject 5 0 3 0 2 Subject 6 0 2 0 One Subject 7 0 3 0 3 Subject 8 0 3 0 2 Subject 9 0 2 0 3 Subject 10 0 2 0 One DELTA W (T8-T0) 2.5 2.0

Visual Evaluation Results In Example 1, the objective evaluation by the skilled person and the subjective evaluation by the subject were 2.7 and 2.5, respectively, indicating that the effect of improving wrinkles is excellent. This indicates that Example 1 of the present invention showed an increase of 50% in the objective evaluation of the expert and 25% in the subjective evaluation of the subject compared to Comparative Example 1 in which the conventional liposome containing the microalgae extract was contained. It can be seen that Example 1 containing the algae of the present invention improves the wrinkles very effectively.

[ Experimental Example  4] Skin elasticity improvement effect

The skin elasticity enhancing effect of the cosmetic containing the alginate containing the microalgae extract of the present invention was measured.

Twenty women (mean age 35.2 years) older than 20 years were divided into two groups (A and B) under the conditions of temperature of 24 to 26 캜 and humidity of 75%, and Example 1 was applied to A group, The skin elasticity was measured using a skin elasticity tester (Cutometer SEM 575, C + K Electronic Co., Germany) after applying Comparative Example 1 around the eye area for 8 weeks (twice / day). The test results are shown in the following Table 6 as the value of? R8 (R8 (8 weeks) -R8 (0 weeks)) of Cutometer SEM 575. The R8 value indicates the nature of viscoelasticity of the skin.

Experimental product R8 Example 1 0.47 Comparative Example 1 0.30

As can be seen from the results of Table 6 above, the cosmetic composition (Example 1) containing the algae containing the microalgae extract of the present invention was superior to Comparative Example 1 containing the existing liposome containing microalgae extract The elasticity increased by 56.7%, indicating a high skin elasticity improvement effect.

[ Experimental Example  5] Skin moisturizing effect

The skin moisturizing effect of the cosmetic containing the algae containing the microalgae extract of the present invention was measured.

Measurements were made after stabilizing the skin at 24-26 ° C, 45% relative humidity and 20-30 minutes in the absence of air flow. The cosmetic composition of Example 1 according to the present invention was divided into group A and the cosmetic composition according to Comparative Example 1 was divided into groups B and 20, (2 times / day) for 6 weeks. The transepidermal water loss (TEWL) value was measured using a TEWAMETER TM210 (C + K electronic GmbH, Germany) with a change in the TEWL value ΔTEWL with time, and the change was measured using a CORNEOMETER CM 820 PC . The moisture content of the skin was quantified from 0 to 150 by the change of the electrical conductivity according to the moisture content of the skin using the. The test results are shown in Table 7 below.

Test Products △ TEWL Corneometer Value Example 1 1.5 118 Comparative Example 1 4.6 85

As a result of the test, the ΔTEWL value of Example 1 containing algae containing microalgae extract was 1.5 g / hm ² , which means that the transdermal water loss was significantly improved after 6 weeks compared to Comparative Example 1. In addition, the corneometer value obtained by measuring the moisture content of the skin showed that the skin moisturizing effect of Example 1 was increased by 28.0% than that of the Comparative Example.

[ Experimental Example  6] Skin penetration effect

The skin penetration effect of the cosmetic composition containing the alginate containing the microalgae extract of the present invention was measured.

Human normal fibroblast 1 × 10 ⁵ cells / ml Cells were injected into a collagen gel structure similar to the fibrous tissue of the human body. Collagen solution 3 mg / ml: 5 × DMEM: 2.2% Sodium bicarbonate and 200 mM Dermal Equivalent (DE), obtained by inoculation in a 7: 2: 1 mixture of 0.05N sodium hydroxide containing Hepes buffer and 5% CO ₂ at 37 ° C for 7 days, was treated with 3 μm porous polycarbonate 1 × 10 ⁵ cells / ml of epidermal keratinocyte on the surface of Dermal Equivalent (DE) where fibroblasts were cultured in the inside of the plates separated from each other by a membrane made of a material (Membrane) And K-SFM medium containing carotenoids and BPE is placed on the inside and outside, and cultured for 7 days. Then, the medium inside the plate was discarded so that the air was brought into contact with the surface of the cultured cells, and K-SFM containing 10% FBS and DMEM without carnoid were mixed in the same medium for 2 weeks, Of artificial skin with epidermis and dermis.

Example 1 and Comparative Example 1 of the present invention were applied to artificial dermal tissue of the uppermost layer and cultured for 4 hours. Then, in Example 1 (Example 1) in which the epidermis and dermis of the artificial skin were permeated through DMEM medium containing no carti- And the amount of carotenoid in the microalgae extract contained in the liposome of Comparative Example 1 was analyzed by TLC. At this time, carnitoid was added to the medium so that the carnitide content in the microalgae extract was 200 μg / 1 ml, and the algae of Example 1 and the liposome of Comparative Example 1 were prepared so that the microalgae extract contained 10.0% of the total weight. Test results for the skin penetration effect are shown in Table 8 below.

The amount of EGF in the medium (μg / ml) Example 1 30.7 Comparative Example 1 12.3

From the above results, it can be seen that the algae containing the microalgae extract has a higher penetration effect than the microalgae extract in the general liposome.

This is because the particle size of algae containing microalgae extract is small and it is more affinity to the skin, and the manufacturing process proceeds at room temperature to maintain the high activity of the algae on the stability of the heat-sensitive cartinoids, .

[ Experimental Example  7] Cosmetics  Formulation stability of the composition

The formulation stability of the cosmetic composition containing the algae containing the microalgae extract of the present invention was measured.

To test the formulation stability of the cosmetic, Example 1 and Comparative Example 1 of the present invention were placed in an opaque glass container in a thermostat kept constant at 45 DEG C and stored for 12 weeks, and the completely shaded After storage for 12 weeks in an opaque glass container in a refrigerator, the extent of discoloration and degree of discoloration were compared. The results are shown in Table 9 below. At this time, the degree of product separation and discoloration was classified into the following six grades and evaluated.

Product Discoloration Evaluation Criteria:

0: No change 1: Very little separation (discoloration)

2: slightly separated (discolored) 3: slightly discolored (discolored)

4: Severely separated (discolored) 5: Severely separated (discolored)

Temperature Degree of discoloration of test substance Example 1 Comparative Example 1 45 ° C 0 1.9 4 ℃ 0 0

As can be seen in Table 9 above, Example 1 containing algae was stable at 4 &lt; 0 &gt; C and 45 &lt; 0 &gt; C with no discoloration or separation symptoms, whereas Comparative Example 1, which contained normal liposomes, But it is not stable at 45 ℃.

[ Experimental Example  8] Skin Stability Experiment

Skin safety tests were conducted on cosmetics containing algae containing the microalgae extract of the present invention.

Twenty subjects (mean age 26.7 years, age range 18 to 30 years) were subjected to a skin patch test on the upper limb using Haye's Test Chamber of Example 1 and Comparative Example 1 of the present invention. Patients with psoriasis, eczema, other skin lesions, pregnant, lactating or contraceptive, and antihistamines were excluded from the study. The test site is wiped with 70% ethanol and dried, and 15 μg of each of Example 1 and Comparative Example 1 is dropped on the chamber, and then placed on the upper part of the test site to be fixed. After applying the patch for 24 hours and removing the patch, mark the test area with Marking Pen and observe the test area after 24 hours and 48 hours, respectively. The determination was carried out 24 hours and 48 hours after the removal of the patch, and the skin reaction was judged according to the rules of the International Contact Dermatitis Research Group (ICDRG) shown in Table 10 below. The test results are shown in Table 11 below as skin stability.

sign Criteria evaluation Mean Score ±
+
++
+++
- Doubtful or slight reaction and erythema
Erythema + Induration
Erythema + Induration + Vesicle
Erythema + Induration + Bullae
Negative Small stimulus
Light stimulus
Moderate irritation
River stimulation
No stimulation 0 to 0.9
1.0 to 2.9
3.0 ~ 4.9
5.0 or higher
0

Elapsed time Example 1 Comparative Example 1 24 hours 0 0.0 48 hours 0 0.0

 As a result of the skin safety test in Table 11, it can be seen that both the example 1 and the comparative example 1 have a mean degree of irritation of 0 and are a safe cosmetic without irritation to the skin.

Based on the results of the above-mentioned experimental examples, cosmetics containing algae containing microalgae extracts are presented. However, the composition of the present invention is not intended to be limited to the following formulation examples.

[ Formulation Example  1] Softening longevity (skin lotion)

 Compounding ingredient weight%  Production Example 2 1.0  1,3-butylene glycol 6.0  glycerin 4.0  Oleyl alcohol 0.1  Polysorbate 20 0.5  ethanol 15.0  Benzophenone-9 0.05  Incense, preservative a very small amount  Purified water to 100

[ Formulation Example  2] Nourishing lotion ( Milk lotion )

Compounding ingredient weight%  Production Example 2 3.0  Propylene glycol 6.0  glycerin 4.0  Triethanolamine 1.2  Tocopheryl acetate 3.0  Liquid paraffin 5.0  Squalane 3.0  Macadamia nut oil 2.0  Polysorbate 60 1.5  Sorbitan sesquioleate 1.0  Carboxyvinyl polymer 1.0  Viechti 0.01  EDITEE-2 EN 0.01  Incense, preservative a very small amount  Purified water to 100

[ Formulation Example  3] Nourishing cream

Compounding ingredient weight%  Production Example 2 10.0  Cetostearyl alcohol 2.0  Glyceryl stearate 1.5  Trioctanoin 5.0  Polysorbate 60 1.2  Sorbitan stearate 0.5  Squalane 5.0  Liquid paraffin 3.0  Cyclomethicone 3.0  Viechti 0.05  Delta-tocopherol 0.2  Concentrated glycerin 4.0  1,3-butylene glycol 2.0  Xanthan gum 0.1  EDITEE-2 EN 0.05  Incense, preservative a very small amount  Purified water to 100

[ Formulation Example  4] Massage cream

 Compounding ingredient weight%  Production Example 2 3.0  Propylene glycol 6.0  glycerin 4.0  Triethanolamine 0.5  Wax 2.0  Tocopheryl acetate 0.1  Polysorbate 60 3.0  Sorbitan sesquioleate 2.5  Cetearyl alcohol 2.0  Liquid paraffin 30.0  Carboxyvinyl polymer 0.5  Incense, preservative a very small amount  Purified water to 100

[ Formulation Example  5] Pack

 Compounding ingredient weight%  Production Example 2 2.0  Propylene glycol 2.0  glycerin 4.0  Carboxyvinyl polymer 0.3  ethanol 7.0  Piggy-40 Hydrogenated Castor Oil 0.8  Triethanolamine 0.3  Viechti 0.01  EDITEE-2 EN 0.01  Incense, preservative a very small amount  Purified water to 100

Claims (7)

A cosmetic composition containing algae containing a microalgae extract in a lipid complex. 2. The cosmetic composition according to claim 1, wherein the algae contains a microalgae extract in a lipid complex consisting of phospholipid, phytosphingosine, ceramide 3, cholesterol, phytosterol, beta-sitosterol and squalene. The composition of claim 2, wherein the algae comprises 0.01-30.0 wt.% Phospholipid, 0.01-10.0 wt.% Phytosphingosine, 0.01-50.0 wt.% Ceramide 3, 0.01-30.0 wt.% Cholesterol, 0.01-10.0 wt% of phytosterol, 0.01-10.0 wt% of beta-sitosterol, 0.01-80.0 wt% of squalene and 0.01-50.0 wt% of microalgae extract. The cosmetic composition according to claim 1, wherein the cosmetic composition has at least one of a skin wrinkle improving effect, a skin elasticity improving effect and a skin moisturizing effect. The cosmetic composition according to claim 1, wherein the algae are contained in an amount of 0.01-100.0 wt% based on the total weight of the total cosmetic composition. The method of claim 1, wherein the algesome is prepared by dissolving phospholipid, phytosphingosine, ceramide 3, cholesterol, phytosterol, beta-sitosterol, and squalene at high pressure to form a lipid complex in the form of a translucent gel, Lipid complex, and a microalgae extract. &Lt; RTI ID = 0.0 &gt; 21. &lt; / RTI &gt; The cosmetic composition according to claim 1, wherein the microalgae extract is an extract of Dunaliella Salina .

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