KR102594647B1 - Functional cosmetic composition containing a mixed extract of microalgae as an active ingredient - Google Patents

Abstract

The present invention relates to a functional cosmetic composition containing a mixed extract of microalgae as an active ingredient. Specifically, among microalgae, Euglena gracilis , Chlorella Pyrenoidosa, Chlorella protothecoides, Dunaliella Salina , and Dunaliella Bardawil. A mixed extract using Bardawil , Spirulina Platensis , and Spirulina subsalsa has excellent antioxidant and whitening effects, so it can be used as a cosmetic composition.

Description

Functional cosmetic composition containing a mixed extract of microalgae as an active ingredient}

The present invention relates to a functional cosmetic composition containing a mixed extract of microalgae as an active ingredient.

The main cause of skin aging is known to be free radicals. Antioxidants are substances that protect the skin surface from oxidative damage caused by environmental invaders such as free radicals, ultraviolet rays, and the environment. In other words, antioxidants are molecules that reduce the effect of free oxygen and supply electrons to free radicals to reduce their reactivity. When free radicals overload our bodies, oxidative stress occurs, an imbalance between the body's ability to detoxify harmful effects and the production of free radicals. Antioxidants to counteract this imbalance include vitamin C, which is known to brighten the skin and minimize dark spots associated with photoaging often caused by UV damage, and vitamin A (retinol), which is known to stimulate the production of skin cells. and niacinamide, which is known to protect against free radical damage that causes premature aging.

Generally, algae are classified based on their size into macroalgae, which grow up to tens of meters, such as seaweed, seaweed, and kelp, and microalgae, which are single-celled organisms several micrometers in size, such as phytoplankton. At this time, microalgae called phytoplankton are one of the first organisms that appeared in the Earth's oceans about 3.5 billion years ago.

Meanwhile, microalgae are a type of phytoplankton and are microorganisms based on photoautotrophic carbon metabolism, or photosynthesis, that use sunlight as an energy source and carbon dioxide as a carbon source. Most of them are single-celled organisms that are usually a few to hundreds of micrometers in size, but there are also species that are connected in chains or in groups, reaching an overall size of several meters. Microalgae include algae and algae, which are eukaryotes, and Cyanobacteria (aka 'Blue-green algae'), which are prokaryotes. Microalgae are generally based on photoautotrophic metabolism using light and carbon dioxide (CO ₂ ), but in some cases, they may use some organic matter as a carbon source instead of carbon dioxide, and in this case, they can grow without light (heterotrophic). Additionally, depending on the species used and culture conditions, CO ₂ and organic matter can be consumed simultaneously under light conditions (Mixotrophic). Carbon dioxide not only uses gaseous _CO2 , but can also consume dissolved inorganic carbon in the form of bicarbonate ( ^HCO3- ) dissolved in water.

The traditional industrial use of microalgae is in the environmental field. This is because microalgae consume carbon (C), nitrogen (N), and phosphorus (P) as nutrients as they grow, so from a human perspective, these are pollutants. In some cases, it is used to purify polluted water or air. In addition, microalgae biomass contains proteins, fats, carbohydrates, etc. and produces secondary metabolites during the growth process. These substances are used as raw materials in various industries such as food and energy depending on their characteristics.

In the present invention, the microalgae Euglena gracilis , Chlorella Pyrenoidosa, Chlorella protothecoides, Dunaliella Salina , Dunaliella Bardawil ( Dunaliella ) The present invention was completed by developing a functional cosmetic composition with antioxidant and whitening activity using Bardawil , Spirulina Platensis , and Spirulina subsalsa .

10-2147482 10-2008870

One object of the present invention to solve the above problems is to provide an antioxidant cosmetic composition containing a mixed extract of microalgae as an active ingredient.

Another object of the present invention is to provide a cosmetic composition with enhanced whitening function containing a mixed extract of microalgae as an active ingredient.

In order to achieve the above object, the present invention relates to an antioxidant cosmetic composition containing a mixed extract of microalgae as an active ingredient, wherein the microalgae include Euglena gracilis and Chlorella Pyrenoidosa . ), Chlorella protothecoides , Dunaliella Salina, Dunaliella Bardawil, Spirulina Platensis , and Spirulina subsalsa . The above provides a cosmetic composition for antioxidant use.

In one embodiment of the present invention, the "composition" is Euglena gracilis , Chlorella Pyrenoidosa , Chlorella protothecoides, Dunaliella Salina , Dunaliella Bardawil , Spirulina Platensis and Spirulina subsalsa 0.5~3: 0.5~3: 0.5~4: 0.5~4: 0.5~2: 0.5~ It may be mixed at a weight ratio of 3:0.5 to 3, but is not limited to this.

In one embodiment of the present invention, the “composition” may have an anti-aging effect on skin through antioxidants, but is not limited thereto.

In one embodiment of the present invention, the “composition” may have whitening activity, but is not limited thereto.

In one embodiment of the present invention, the "composition" includes skin lotion, skin softener, skin toner, astringent, lotion, milk lotion, moisture lotion, nutritional lotion, sunscreen, massage cream, nutritional cream, moisture cream, hand cream, It may be one or more formulations selected from formulations consisting of foundation, essence, nutritional essence, cleansing, pack, soap, body lotion, and body cleanser, but is not limited thereto.

The present invention relates to a functional cosmetic composition containing a mixed extract of microalgae as an active ingredient, and more specifically, among microalgae, Euglena gracilis , Chlorella Pyrenoidosa , and Chlorella protothecoides. Extracts were prepared using ( Chlorella protothecoides ), Dunaliella Salina , Dunaliella Bardawil , Spirulina Platensis and Spirulina subsalsa , and their Excellent antioxidant efficacy was confirmed. By preparing a mixed extract according to the antioxidant efficacy of each extract, it was confirmed that the antioxidant efficacy was increased and that it could be used as a functional cosmetic composition with whitening function through its tyrosinase inhibitory effect.

Hereinafter, the present invention will be described in detail through embodiments of the present invention with reference to the attached drawings. However, the following implementation examples are provided as examples of the present invention, and if it is judged that a detailed description of a technology or configuration well known to those skilled in the art may unnecessarily obscure the gist of the present invention, the detailed description may be omitted. , the present invention is not limited thereby. The present invention is capable of various modifications and applications within the description of the patent claims described below and the scope of equivalents interpreted therefrom.

In addition, terminology used in this specification is a term used to appropriately express preferred embodiments of the present invention, and may vary depending on the intention of the user or operator or the customs of the field to which the present invention belongs. Therefore, definitions of these terms should be made based on the content throughout this specification. Throughout the specification, when a part is said to “include” a certain element, this means that it may further include other elements rather than excluding other elements, unless specifically stated to the contrary.

Throughout this specification, '%' used to indicate the concentration of a specific substance means (w/w) % for solid/solid, (w/v) % for solid/liquid, and Liquid/liquid is (v/v) %.

In one aspect, the present invention may be an antioxidant cosmetic composition containing a mixed extract of microalgae as an active ingredient.

The cosmetic composition of the present invention can be used in various ways to prevent skin aging through its antioxidant effect, and the mixture of the present invention additionally has a whitening function. Products to which this composition can be added include, for example, cosmetics such as various creams, skins, lotions, essences, cleansing foam, cleansing lotion, cleansing cream, shampoo, rinse, face wash, soap, treatment, pack, There are cosmetic liquids, etc.

The cosmetic composition of the present invention may further include a composition selected from the group consisting of water-soluble vitamins, oil-soluble vitamins, high molecular weight peptides, high molecular weight polysaccharides, sphingolipids, and seaweed extracts.

Water-soluble vitamins may be any that can be mixed into cosmetics, but preferably include vitamin B1, vitamin B2, vitamin B6, pyridoxine, pyridoxine hydrochloride, vitamin B12, pantothenic acid, nicotinic acid, nicotinic acid amide, folic acid, vitamin C, and vitamin H. and their salts (thiamine hydrochloride, sodium ascorbate, etc.) or derivatives (sodium ascorbic acid-2-phosphate, magnesium ascorbic acid-2-phosphate, etc.) are also water-soluble vitamins that can be used in the present invention. Included. Water-soluble vitamins can be obtained by conventional methods such as microbial transformation, purification from microbial cultures, enzymatic methods, or chemical synthesis.

Oil-soluble vitamins may be any that can be mixed into cosmetics, but preferably include vitamin A, carotene, vitamin D2, vitamin D3, vitamin E (d1-alpha tocopherol, d-alpha tocopherol, d-alpha tocopherol), etc. , their derivatives (ascorbic palmitate, ascorbic stearate, ascorbic acid dipalmitate, dl-alpha tocopherol acetate, dl-alpha tocopherol nicotinic acid, vitamin E, DL-pantothenyl alcohol, D-pantothenyl alcohol, pantothenyl ethyl ether, etc.) are also included in the oil-soluble vitamins used in the present invention. Oil-soluble vitamins can be obtained by conventional methods such as microbial transformation, purification from microbial cultures, enzymatic or chemical synthesis.

The polymer peptide may be any peptide that can be blended into cosmetics, but preferably includes collagen, hydrolyzed collagen, gelatin, elastin, hydrolyzed elastin, and keratin. High molecular weight peptides can be purified and obtained by conventional methods such as purification from microbial culture media, enzymatic methods, or chemical synthesis, or they can usually be purified and used from natural products such as dermis of pigs or cows or silk fiber of silkworms.

The polymer polysaccharide may be any one that can be blended into cosmetics, but preferably includes hydroxyethyl cellulose, xanthan gum, sodium hyaluronate, chondroitin sulfate or its salts (sodium salt, etc.). For example, chondroitin sulfate or its salt can usually be purified and used from mammals or fish.

The sphingolipid may be any one that can be incorporated into cosmetics, but preferably includes ceramide, phytosphingosine, and sphingolipid. Sphingolipids can usually be purified by conventional methods from mammals, fish, shellfish, yeast, or plants, or obtained by chemical synthesis.

The seaweed extract may be any one that can be blended into cosmetics, but preferably includes brown algae extract, red algae extract, green algae extract, etc. Also, calrageenan, arginic acid, sodium alginate, etc. purified from these seaweed extracts. Potassium arginate and the like are also included in the seaweed extract used in the present invention. Seaweed extract can be obtained by purifying seaweed by a conventional method.

In addition to the above-mentioned essential ingredients, the cosmetic of the present invention may contain other ingredients usually blended in cosmetics as needed. Other ingredients that may be added include oils and fats, moisturizers, emollients, surfactants, organic and inorganic pigments, organic powders, ultraviolet absorbers, preservatives, disinfectants, antioxidants, plant extracts, pH adjusters, alcohol, colorants, fragrances, Examples include blood circulation promoters, cooling agents, antiperspirants, and purified water. Examples of oil and fat components include ester-based fats and oils, hydrocarbon-based fats and oils, silicone-based fats and oils, fluorine-based fats and oils, animal fats and oils, and vegetable fats and oils.

Ester-based oils include glyceryl tri2-ethylhexanoate, cetyl 2-ethylhexanoate, isopropyl myristate, butyl myristate, isopropyl palmitate, ethyl stearate, octyl palmitate, isocetyl isostearate, and stearic acid. Butyl, ethyl linoleate, isopropyl linoleate, ethyl oleate, isocetyl myristate, isostearyl myristate, isostearyl palmitate, octyldodecyl myristate, isocetyl isostearate, diethyl sebacate, adipine. Diisopropyl acid, isoalkyl neopentanoate, tri(caprylic, capric acid)glyceryl, trimethylolpropane tri2-ethylhexanoate, trimethylolpropane triisostearate, pentaelislitol tetra2-ethylhexanoate , cetyl caprylate, decyl laurate, hexyl laurate, decyl myristate, myristyl myristate, cetyl myristate, stearyl stearate, decyl oleate, cetyl ricinooleate, isostear laurate. Reyl, isotridecyl myristate, isocetyl palmitate, octyl stearate, isocetyl stearate, isodecyl oleate, octyldodecyl oleate, octyldodecyl linoleate, isopropyl isostearate, cetoester 2-ethylhexanoate Aryl, 2-ethylhexanoate stearyl, hexyl isostearate, ethylene glycol dioctanate, ethylene glycol dioleate, propylene glycol dicapric acid, di(capryl, capric acid) propylene glycol, propylene glycol dicaprylate, dicapric acid Neopentyl glycol prinate, neopentyl glycol dioctanate, glyceryl tricaprylate, glyceryl triundecylate, glyceryl triisopalmitate, glyceryl triisostearate, octyldodecyl neopentanoate, isostear octanoate. Reyl, octyl isononanoate, hexyldecyl neodecanoate, octyldodecyl neodecanoate, isocetyl isostearate, isostearyl isostearate, octyldecyl isostearate, polyglycerol oleic acid ester, polyglycerol isostearic acid ester. , Triisocetyl citrate, Triisoalkyl citrate, Triisooctyl citrate, Lauryl lactate, Myristyl lactate, Cetyl lactate, Octyldecyl lactate, Triethyl citrate, Acetyltriethyl citrate, Acetyltributyl citrate, Trioctyl citrate, Mal. Diisostearyl acid, 2-ethylhexyl hydroxystearate, di2-ethylhexyl succinate, diisobutyl adipate, diisopropyl sebacate, dioctyl sebacate, cholesteryl stearate, cholesteryl isostearate. , cholesteryl hydroxystearate, cholesteryl oleate, dihydrocholesteryl oleate, phytsteryl isostearate, phytsteryl oleate, 12-stealoyl hydroxystearate isocetyl, 12-stealoyl Ester systems such as stearyl monohydroxystearate and isostearyl 12-stealoylhydroxystearate can be mentioned.

Hydrocarbon-based fats and oils include squalene, liquid paraffin, alpha-olefin oligomer, isoparaffin, ceresin, paraffin, liquid isoparaffin, polybudene, microcrystalline wax, and petroleum jelly.

Silicone oils include polymethyl silicone, methylphenyl silicone, methylcyclopolysiloxane, octamethylpolysiloxane, decamethylpolysiloxane, dodecamethylcyclosiloxane, dimethylsiloxane and methylcetyloxysiloxane copolymer, dimethylsiloxane and methylstealoxysiloxane copolymer, and alkyl. Modified silicone oil, amino-modified silicone oil, etc. can be mentioned.

Examples of fluorine-based fats and oils include perfluoropolyether.

Animal or plant oils include avocado oil, almond oil, olive oil, sesame oil, rice bran oil, birdflower oil, soybean oil, corn oil, rapeseed oil, apricot oil, palm kernel oil, palm oil, castor oil, sunflower oil, and grape seed oil. , cottonseed oil, palm oil, cucumber nut oil, wheat germ oil, rice germ oil, shea butter, walnut colostrum oil, marker damia nut oil, meadow oil, egg yolk oil, beef tallow, horse oil, mink oil, orange rape oil, jojoba oil. , animal or plant oils such as candelier wax, carnaba wax, liquid lanolin, and hydrogenated castor oil.

Moisturizers include water-soluble low-molecular-weight moisturizers, oil-soluble molecular moisturizers, water-soluble polymers, and fat-soluble polymers.

Water-soluble low-molecular-weight moisturizers include serine, glutamine, sorbitol, mannitol, sodium pyrrolidone-carboxylate, glycerin, propylene glycol, 1,3-butylene glycol, ethylene glycol, polyethylene glycol B (degree of polymerization n = 2 or more), and polypropylene. Examples include glycol (degree of polymerization n = 2 or more), polyglycerin B (degree of polymerization n = 2 or more), lactic acid, lactate, etc.

Examples of fat-soluble low-molecular-weight moisturizers include cholesterol and cholesterol esters.

Water-soluble polymers include carboxyvinyl polymer, polyaspartate, tragacanth, xanthan gum, methylcellulose, hydroxymethylcellulose, hydroxyethylcellulose, hydroxypropylcellulose, carboxymethylcellulose, water-soluble chitin, chitosan, dextrin, etc. You can.

Examples of oil-soluble polymers include polyvinylpyrrolidone and eicosene copolymers, polyvinylpyrrolidone and hexadecene copolymers, nitrocellulose, dextrin fatty acid ester, and polymer silicone. Examples of emollients include long-chain acyl glutamic acid cholesteryl ester, hydroxystearic acid cholesteryl, 12-hydroxystearic acid, stearic acid, rosin acid, and lanolin fatty acid cholesteryl ester.

Examples of surfactants include nonionic surfactants, anionic surfactants, cationic surfactants, and amphoteric surfactants.

Nonionic surfactants include self-emulsifying glycerin monostearate, propylene glycol fatty acid ester, glycerin fatty acid ester, polyglycerin fatty acid ester, sorbitan fatty acid ester, POE (polyoxyethylene) sorbitan fatty acid ester, POE sorbitan fatty acid ester, POE. Glycerin fatty acid ester, POE alkyl ether, POE fatty acid ester, POE hydrogenated castor oil, POE castor oil, POE and POP (polyoxyethylene and polyoxypropylene) copolymer, POE and POP alkyl ether, polyether modified silicone, lauric acid. Examples include alkanolamide, alkylamine oxide, and hydrogenated soybean phospholipid.

Anionic surfactants include fatty acid soap, alpha-acyl sulfonate, alkyl sulfonate, alkyl allyl sulfonate, alkylnaphthalene sulfonate, alkyl sulfate, POE alkyl ether sulfate, alkyl amide sulfate, alkyl phosphate, POE alkyl phosphate industry, alkyl amide. Phosphate, alkyloyl alkyl taurine salt, N-acylamino acid salt, POE alkyl ether carboxylate, alkyl sulfosuccinate, sodium alkyl sulfoacetate, acylated hydrolyzed collagen peptide salt, perfluoroalkyl phosphate ester, etc. there is.

Cationic surfactants include alkyltrimethylammonium chloride, stearyltrimethylammonium chloride, stearyltrimethylammonium bromide, cetostearyltrimethylammonium chloride, distearyldimethylammonium chloride, stearyldimethylbenzylammonium chloride, behenyltrimethylammonium bromide, and cationic surfactant. Examples include benzalkonium, diethylaminoethylamide stearate, dimethylaminopropyl stearate, and quaternary ammonium salts of lanolin derivatives. Amphoteric surfactants include carboxy beta type, amide beta type, sulfo beta type, hydroxy sulfo beta type, amide sulfo betaine type, phosphobetain type, aminocarboxylate type, imidazoline derivative type, and amidamine type. Amphoteric surfactants, etc. can be mentioned.

Organic and inorganic pigments include silicic acid, anhydrous silicic acid, magnesium silicate, talc, sericite, mica, kaolin, bengala, clay, bentonite, titanium-coated mica, bismuth oxychloride, zirconium oxide, magnesium oxide, zinc oxide, titanium oxide, and aluminum oxide. Inorganic pigments such as calcium sulfate, barium sulfate, magnesium sulfate, calcium carbonate, magnesium carbonate, iron oxide, ultramarine blue, chromium oxide, chromium hydroxide, calamine and complexes thereof; Polyamide, polyester, polypropylene, polystyrene, polyurethane, vinyl resin, urea resin, phenolic resin, fluorine resin, silicon resin, acrylic resin, melamine resin, epoxy resin, polycarbonate resin, divinylbenzene and styrene copolymer, Examples include organic pigments such as silk powder, cellulose, CI pigment yellow, and CI pigment orange, and complex pigments of these inorganic pigments and organic pigments.

Examples of organic powder include metal soap such as calcium stearate; Alkyl phosphate metal salts such as sodium zinc cetilate, zinc laurylate, and calcium laurylate; Acyl amino acid polyvalent metal salts such as N-lauroyl-beta-alanine calcium, N-lauroyl-beta-alanine zinc, and N-lauroyl glycine calcium; Amidesulfonic acid polyvalent metal salts such as N-lauroyl-taurine calcium and N-palmitoyl-taurine calcium; N such as N-epsilon-lauroyl-L-lysine, N-epsilon-palmitoylizine, N-alpha-paritoylolnithine, N-alpha-lauroylarginine, N-alpha-hydrogenated beef tallow fatty acid acylarginine, etc. -Acyl basic amino acid; N-acyl polypeptides such as N-lauroylglycylglycine; Alpha-amino fatty acids such as alpha-aminocaprylic acid and alpha-aminolauric acid; Examples include polyethylene, polypropylene, nylon, polymethyl methacrylate, polystyrene, divinylbenzene and styrene copolymers, and ethylene tetrafluoride.

UV absorbers include para-aminobenzoic acid, ethyl para-aminobenzoate, amyl para-aminobenzoate, octyl para-aminobenzoate, ethylene glycol salicylate, phenyl salicylate, octyl salicylate, benzyl salicylate, butylphenyl salicylate, homomenthyl salicylate, and benzyl cinnamate. , paramethoxycinnamic acid-2-ethoxyethyl, octyl paramethoxycinnamic acid, mono-2-ethylhexane glyceryl diparamethoxycinnamic acid, isopropyl paramethoxycinnamic acid, diisopropyl and diisopropylcinnamic acid ester mixture, Urocanic acid, ethyl urocanic acid, hydroxymethoxybenzophenone, hydroxymethoxybenzophenone sulfonic acid and its salts, dihydroxymethoxybenzophenone, dihydroxymethoxybenzophenone disulfonate sodium, dihydroxybenzoate Phenone, tetrahydroxybenzophenone, 4-tert-butyl-4'-methoxydibenzoylmethane, 2,4,6-trianilino-p-(carbo-2'-ethylhexyl-1'-oxy)- 1,3,5-triazine, 2-(2-hydroxy-5-methylphenyl)benzotriazole, etc. are mentioned.

Disinfectants include hinokitiol, triclosan, trichlorohydroxydiphenyl ether, chlorhexidine gluconate, phenoxyethanol, resorcin, isopropylmethylphenol, azulene, salicylic acid, zincphyllithione, benzalkonium chloride, and photosensitive. Sub-No. 301, mononitroguaiacol sodium, undecirenic acid, etc. can be mentioned.

Antioxidants include butylhydroxyanisole, propyl gallate, and elisorbic acid.

Examples of pH adjusters include citric acid, sodium citrate, malic acid, sodium malate, fumal acid, sodium fumalate, succinic acid, sodium succinate, sodium hydroxide, sodium monohydrogen phosphate, and the like.

Examples of alcohol include higher alcohols such as cetyl alcohol.

Additionally, the ingredients that may be added are not limited to this, and any of the above ingredients can be blended within the range that does not impair the purpose and effect of the present invention.

The cosmetic of the present invention may take the form of a solution, emulsion, viscous mixture, etc.

The ingredients included in the cosmetic composition of the present invention may include ingredients commonly used in cosmetic compositions as active ingredients, for example, conventional auxiliaries and carriers such as stabilizers, solubilizers, vitamins, pigments, and fragrances. Includes.

The functional cosmetic composition for preventing skin aging or whitening skin through antioxidants of the present invention can be manufactured in any formulation commonly manufactured in the art, for example, emulsion, cream, lotion, pack, foundation, lotion, beauty essence, hair Cosmetics, etc. can be mentioned.

Specifically, the cosmetic composition of the present invention includes skin lotion, skin softener, skin toner, milk lotion, astringent, lotion, moisture lotion, nutritional lotion, massage cream, nutritional cream, moisture cream, hand cream, foundation, essence, nutritional essence, It includes formulations of packs, soaps, cleansing foams, cleansing lotions, cleansing creams, hair lotions, hair tonics, hair essences, hair shampoos, hair rinses, hair treatments, body lotions, and body cleansers.

When the formulation of the present invention is a paste, cream or gel, animal fiber, plant fiber, wax, paraffin, starch, tracant, cellulose derivative, polyethylene glycol, silicone, bentonite, silica, talc or zinc oxide may be used as the carrier ingredient. You can.

When the formulation of the present invention is a powder or spray, lactose, talc, silica, aluminum hydroxide, calcium silicate, or polyamide powder can be used as the carrier ingredient. In particular, when the formulation is a spray, chlorofluorohydrocarbon and propane may be used as carrier ingredients. /May contain propellants such as butane or dimethyl ether.

When the formulation of the present invention is a solution or emulsion, a solvent, solvating agent or emulsifying agent is used as a carrier component, such as water, ethanol, isopropanol, ethyl carbonate, ethyl acetate, benzyl alcohol, benzyl benzoate, propylene glycol, 1 , 3-butyl glycol oil, glycerol aliphatic esters, polyethylene glycol or fatty acid esters of sorbitan.

When the formulation of the present invention is a suspension, the carrier ingredients include water, a liquid diluent such as ethanol or propylene glycol, a suspending agent such as ethoxylated isostearyl alcohol, polyoxyethylene sorbitol ester, and polyoxyethylene sorbitan ester, and microcrystalline Cellulose, aluminum metahydroxide, bentonite, agar, or tracant may be used.

When the formulation of the present invention is a surfactant-containing cleansing agent, the carrier ingredients include aliphatic alcohol sulfate, aliphatic alcohol ether sulfate, sulfosuccinic acid monoester, isethionate, imidazolinium derivative, methyl taurate, sarcosinate, and fatty acid amide. Ether sulfate, alkylamidobetaine, fatty alcohol, fatty acid glyceride, fatty acid diethanolamide, vegetable oil, linoline derivative, or ethoxylated glycerol fatty acid ester can be used.

Hereinafter, embodiments of the present invention will be described in more detail with reference to the attached drawings. However, the following examples are only intended to embody the content of the present invention, and the present invention is not limited thereto.

<Preparation Example 1> Preparation of microalgae extract

Microalgae Euglena gracilis , Chlorella Pyrenoidosa , Chlorella protothecoides, Dunaliella Salina , Dunaliella Bardawil , After culturing Spirulina Platensis or Spirulina subsalsa in a photobioreactor, the culture medium was removed, dried, and extracted by adding water, 40% ethanol, or 70% ethanol to the pulverized powder, respectively. Each microalgae extract was obtained by performing. At this time, 5 ml of extraction solvent was added per 1 g of dry weight of microalgae powder, and extraction was performed by shaking a total of 3 times for 3 hours at room temperature. After extraction, only the extract was recovered through filtration, and a microalgae extract was prepared by concentrating the recovered extract using a rotary vacuum concentrator. The microalgae extract prepared in this way was used in the following experiments.

<Experimental Example 1> Measurement of cytotoxicity of microalgae extract

In this Experimental Example 1, we attempted to confirm the cytotoxicity of each microalgae extract prepared in Preparation Example 1 through MTT assay. Human keratinocytes (HaCaT) were inoculated into each 96-well plate at a concentration of 1 × 10 ⁵ cells/ml and cultured in an incubator under 37°C and 5% CO ² conditions. The microalgae extract prepared in Preparation Example 1 was diluted to a concentration of 1 and 100 ㎍/㎖, then treated with each well-plate in which the human keratinocytes were cultured, and added in an incubator under the same conditions for 24 hours. It was cultured. After 24 hours, cell viability was measured using MTT reagent to evaluate cytotoxicity. At this time, keratinocytes that were not treated with the sample were cultured in the same manner and used as a negative control. Absorbance was measured at 540 nm, and the experimental results calculated using Equation 1 below are shown in Table 1 below.

[Equation 1]

Cell viability(%) = Abssample/Abscontrol × 100

extract cell viability
(% of Control) microalgae menstruum Volume Euglena gracilis water One 101.22 water 100 100.11 40% ethanol One 98.21 40% ethanol 100 99.32 70% ethanol One 87.25 70% ethanol 100 87.24 Chlorella Pyrenoidosa water One 98.12 water 100 99.28 40% ethanol One 101.43 40% ethanol 100 98.82 70% ethanol One 91.35 70% ethanol 100 88.22 Chlorella protothecoides water One 100.02 water 100 102.12 40% ethanol One 99.82 40% ethanol 100 101.24 70% ethanol One 94.32 70% ethanol 100 93.82 Dunaliella Salina water One 99.32 water 100 99.01 40% ethanol One 98.03 40% ethanol 100 97.82 70% ethanol One 88.13 70% ethanol 100 83.22 Dunaliella Bardawil water One 101.21 water 100 99.05 40% ethanol One 97.12 40% ethanol 100 98.22 70% ethanol One 87.35 70% ethanol 100 83.22 Spirulina platensis water One 101.11 water 100 100.51 40% ethanol One 99.08 40% ethanol 100 97.22 70% ethanol One 84.23 70% ethanol 100 82.11 Spirulina Salsa water One 10.27 water 100 101.12 40% ethanol One 101.26 40% ethanol 100 99.24 70% ethanol One 92.28 70% ethanol 100 89.11 Control 100

As shown in Table 1 above, it was confirmed that all microalgae extracts did not show cytotoxicity to human keratinocytes.

<Experimental Example 2> Confirmation of antioxidant effect

To evaluate the antioxidant effect of each microalgae extract prepared in Preparation Example 1, DPPH assay was performed. At this time, ascorbic acid (AA) was used as the positive control group. DPPH was dissolved in ethyl alcohol at a concentration of 0.2 mM, 100 μl of the DPPH solution and 100 μl of each sample were reacted for 20 minutes, and then the absorbance (OD) was measured at 517 nm using a spectrophotometer (microplate reader). Additionally, the DPPH radical scavenging ability was calculated using Equation 2 below, and the results are shown in Table 2 below.

[Equation 2]

DPPH inhibition(%) = [1-(Abssample/Abscontrol)] × 100

sample DPPH radical scavenging ability (%) microalgae menstruum Euglena gracilis water 28% 40% ethanol 38% 70% ethanol 19% Chlorella Pyrenoidosa water 33% 40% ethanol 35% 70% ethanol 29% Chlorella protothecoides water 32% 40% ethanol 42% 70% ethanol 39% Dunaliella Salina water 32% 40% ethanol 41% 70% ethanol 39% Dunaliella Bardawil water 22% 40% ethanol 27% 70% ethanol 27% Spirulina platensis water 32% 40% ethanol 39% 70% ethanol 22% Spirulina Salsa water 22% 40% ethanol 34% 70% ethanol 31% ascorbic acid (AA) 67

As can be seen in Table 2 above, it was confirmed that the ethanol extract generally showed a higher antioxidant effect than the water extract, and the extraction with 40% concentration ethanol showed a higher antioxidant effect than the extraction with 70% concentration ethanol. was confirmed.

<Experimental Example 3> Confirmation of antioxidant synergistic effect

In Experimental Example 1, microalgae extracts extracted with 40% ethanol, which has a relatively high antioxidant effect, were mixed together to confirm the synergistic antioxidant effect through DPPH radical scavenging ability. The experimental method was conducted in the same manner as in Experimental Example 2, and the antioxidant synergistic effect was confirmed by mixing 7 samples at various weight ratios (Table 3).

DPPH radical scavenging ability (%)
( Euglena gracilis : Chlorella pyrenoidosa : Chlorella protothecoides : Dunaliella salina : Dunaliella bardawil : Spirulina platensis : Spirulina supsalsa ) Example 1
(1:1:1:1:1:1:1) Example 2
(2:2:3:3:1:2:2) ascorbic acid
(ascorbic acid, AA) 52% 72% 67%

As shown in Table 3, it was confirmed that the antioxidant efficacy was significantly higher than that of the positive control group in the case where each extract was mixed in equal amounts (Example 1) and when the extracts were differentiated and mixed according to antioxidant activity (Example 2).

<Experimental Example 4> Whitening activity test

Next, using Examples 1 and 2, the tyrosinase inhibition effect test, which is widely used in whitening activity tests, was measured using the dopachrome method. After mixing 150 μl of mushroom tyrosinase, 225 μl of 2.5mM L-tyrosine, 225 μl of 0.4M HEPES buffer, and ethanol solution or 300 μl of each sample at a concentration of 1 mg/mL, the absorbance was measured at a wavelength of 475 nm. As a positive control, kojic acid, known as a representative whitening agent, was used. The absorbance of each sample solution measured above was calculated, and the tyrosinase activity inhibition value was calculated using Equation 3 below and shown in Table 4.

[Equation 3]

Tyrosinase Inhibition(%) = [1-(Abssample/Abscontrol)] × 100

Tyrosinase activity inhibition rate (%) Example 1 (1:1:1:1:1:1:1) 52.2% Example 2 (2:2:3:3:1:2:2) 72.4% kojic acid 71.1%

As shown in the results in Table 4 above, Example 2, which shows the best antioxidant efficacy of the present invention, was confirmed to have a superior tyrosinase activity inhibition effect than kojic acid, a positive control group.

<Manufacture example of cosmetics>

The mixed microalgae extract of the present invention can be manufactured into various types of cosmetics depending on the purpose. The following is an example of the manufacturing method of some cosmetics containing the mixed microalgae extract according to the present invention as an active ingredient, and the present invention is not limited thereto.

Manufacturing example of flexible lotion

10.0 parts by weight of mixed microalgae extract

1,3-butylene glycol 1.00 parts by weight

Disodium EDTA 0.05 parts by weight

Allantoin 0.10 parts by weight

Dipotassium glycyrrhizate 0.05 parts by weight

Citric acid 0.01 parts by weight

Sodium citrate 0.02 parts by weight

Glycereth-26 1.00 parts by weight

Arbutin 2.00 parts by weight

Hydrogenated castor oil 1.00 parts by weight

30.0 parts by weight of ethanol

Trace amount of preservative

Trace amount of colorant

Trace amount of flavoring agent

Purified water remaining

Manufacturing example of nutritious cream

10.0 parts by weight of mixed microalgae extract

7.00 parts by weight of 1,3-butylene glycol

Glycerin 1.00 parts by weight

D-Panthenol 0.10 parts by weight

3.20 parts by weight of plant extract

Magnesium aluminum silicate 0.30 parts by weight

PEG-40 stearate 1.20 parts by weight

Stearic acid 2.00 parts by weight

Polysorbate 60 1.50 parts by weight

Lipophilic glyceryl stearate 2.00 parts by weight

Sorbitan sesquioleate 1.50 parts by weight

Cetearyl alcohol 3.00 parts by weight

Mineral oil 4.00 parts by weight

Squalane 3.80 parts by weight

Carlylic/capric triglyceride 2.80 parts by weight

Vegetable oil 1.80 parts by weight

Dimethicone 0.40 parts by weight

Dipotium glycyrrhizate trace amount

Trace amounts of allantoin

Sodium Hyaluronate Trace

Tocopheryl acetate appropriate amount

Triethanolamine dosage

Preservative amount

Flavoring agent appropriate amount

Purified water remaining

Lotion manufacturing example

3.00 parts by weight of mixed microalgae extract

Propylene glycol 6.00 parts by weight

Glycerin 4.00 parts by weight

Triethanolamine 3.00 parts by weight

Tocopheryl acetate 5.00 parts by weight

3.00 parts by weight of liquid paraffin

Squalane 2.00 parts by weight

Macadamia nut oil 2.00 parts by weight

Polysorbate 60 1.50 parts by weight

Sorbitan sesquioleate 1.00 parts by weight

Carboxy vinyl polymer 1.00 parts by weight

Trace amounts of preservatives, fragrance, and coloring

Distilled water remaining

Preparation example of essence

1.50 parts by weight of mixed microalgae extract

Glycerin 10.0 parts by weight

Betaine 5.00 parts by weight

PEG 1500 2.00 parts by weight

Allantoin 0.10 parts by weight

DL-Panthenol 0.30 parts by weight

EDTA-2Na 0.02 parts by weight

Benzophenone-9 0.04 parts by weight

0.10 parts by weight of hydroxyethyl cellulose

Sodium hyaluronate 8.00 parts by weight

0.20 parts by weight of carboxyvinyl polymer

Triethanolamine 0.18 parts by weight

Octyldodecanol 0.30 parts by weight

Octyldodeceth-16 0.40 parts by weight

Ethanol 6.00 parts by weight

Trace amounts of preservatives, fragrance, and coloring

Purified water remaining

Manufacturing example of emulsion for mask pack

1.00 parts by weight of mixed microalgae extract

15.0 parts by weight of polyvinyl alcohol

Cellulose gum 0.15 parts by weight

Glycerin 3.00 parts by weight

PEG 1500 2.00 parts by weight

0.15 parts by weight of cyclodextrin

DL-Panthenol 0.40 parts by weight

Allantoin 0.10 parts by weight

Monoammonium glycyrrhizinate 0.30 parts by weight

Nicotinamide 0.50 parts by weight

Ethanol 6.00 parts by weight

0.30 parts by weight of PEG 40 hydrogenated castor oil

Trace amounts of preservatives, fragrance, and coloring

Purified water remaining

As discussed above, specific embodiments of the present invention have been described in detail, but those skilled in the art who understand the spirit of the present invention may add, change, delete, etc. other components within the scope of the same spirit, thereby creating other regressive inventions. However, other embodiments that are included within the scope of the present invention can be easily proposed. Therefore, the embodiments described above should be understood in all respects as illustrative and not restrictive. The scope of the present invention is indicated by the scope of the claims described below rather than the detailed description above, and all changes or modified forms derived from the meaning and scope of the claims and their equivalent concepts are included in the scope of the present invention. It should be interpreted as

Claims (5)

It relates to an antioxidant cosmetic composition containing a mixed extract of microalgae as an active ingredient,
The microalgae include Euglena gracilis , Chlorella Pyrenoidosa , Chlorella protothecoides, Dunaliella Salina , and Dunaliella Bardawil . , Spirulina Platensis and Spirulina subsalsa mixed in a weight ratio of 0.5~3 : 0.5~3 : 0.5~4 : 0.5~4 : 0.5~2 : 0.5~3 : 0.5~3 A cosmetic composition for antioxidant use.
delete delete According to paragraph 1,
The composition is,
An antioxidant cosmetic composition having whitening activity.
According to paragraph 1,
The composition is,
Skin lotion, skin softener, skin toner, astringent, lotion, milk lotion, moisture lotion, nutritional lotion, sunscreen, massage cream, nutritional cream, moisture cream, hand cream, foundation, essence, nutritional essence, cleansing, pack, soap, body An antioxidant cosmetic composition, characterized in that it is one or more formulations selected from formulations consisting of lotions and body cleansers.