CN113995693B

CN113995693B - Essence composition of liposome with anti-inflammatory effect, and preparation method and application thereof

Info

Publication number: CN113995693B
Application number: CN202111397915.7A
Authority: CN
Inventors: 王勇; 周紫燕; 杨帆; 祝希宇; 郭苗; 周正
Original assignee: Hubei Maishite Biotechnology Co Ltd
Current assignee: Hubei Maishite Biotechnology Co Ltd
Priority date: 2021-11-23
Filing date: 2021-11-23
Publication date: 2023-06-23
Anticipated expiration: 2041-11-23
Also published as: CN113995693A

Abstract

The invention discloses an essence composition of liposome with anti-inflammatory effect and coated with phytochrome and a preparation method thereof. The anti-inflammatory essence composition comprises: 1.5 to 35.0 percent of anti-inflammatory liposome, 5.0 to 40.0 percent of emollient, 0.1 to 30.0 percent of emulsifier, 0.1 to 10.0 percent of thickener, 0.5 to 20 percent of skin conditioner, 0.1 to 10 percent of pH regulator, 0.3 to 5.0 percent of preservative, 0.01 to 1.0 percent of daily essence and the balance of deionized water. The plant pigment composition wrapped by the anti-inflammatory liposome consists of 0.1-10% of lycopene, 0.1-10% of beta-carotene, 0.1-10% of acetoshikonin, 0.1-10% of vitamin E,0.1-10% of vitamin C,0.1-10% of phosphate and 0.1-10% of lecithin, and the components are prepared into lipid inclusion bodies, so that the decomposition of oxidative free radicals in the environment on the plant pigment composition is inhibited, natural plant pigments are protected, the plant pigment composition can be more stable in cosmetics, and on the other hand, the lipid inclusion bodies can better penetrate through sebum membranes, promote the absorption of skin cells to the functional components, and the anti-inflammatory effect is effectively exerted.

Description

Essence composition of liposome with anti-inflammatory effect, and preparation method and application thereof

Technical Field

The invention relates to a liposome composition coated with plant pigment and having anti-inflammatory and antiallergic effects, and a preparation method and application thereof, belonging to the technical field of daily chemicals, and more particularly relates to an essence composition coated with plant pigment and having anti-inflammatory effects, and a preparation method and application thereof.

Background

The skin is an important protective layer for protecting the human body from external injury and stimulation, when the external stimulation causes injury to the human body, the immune system of the skin can start signals, inflammatory factors and histamine of the skin are released, blood flow is accelerated, permeability of blood vessels is increased, skin allergy is caused, and red spots, skin flushing, edema, burning sensation, persistent itching sensation and the like are generated on the skin. If the inflammatory state continues to fail to subside, these excessively released free radicals also attack the collagen and keratinocytes of the skin, causing early stiffening of the collagen to become fragile, stiffening of the skin, and also causing an imbalance in the apoptosis of the keratinocytes, which results in not only desquamation of the skin, but also a rough appearance of the skin, even sagging to produce fine lines.

The method is a primary task for reducing the stimulus source of skin such as damaged skin barrier, acne skin, sensitive skin and the like. The inflammation needs to be prevented from further aggravating, and then the inflammation is gradually diminishing, so that the problems of serious pigmentation and the like are avoided. For healthy skin, the anti-inflammatory effect is more significant in reducing the stimulus and the inflammatory factor, in other words, reducing the free radical, and has great significance for resisting oxidation and delaying aging for skin care.

However, the existing anti-inflammatory and anti-allergic products on the market have various defects of unobvious effect, poor permeability and the like, so that the skin care product which is easier to absorb, better in anti-inflammatory and anti-allergic effects and safer is provided, and the skin care product is a technical problem which is urgently needed to be solved by the current technicians in the field.

Disclosure of Invention

In order to solve the problems in the prior art, the invention aims to provide the essence composition for wrapping the plant pigment liposome with the anti-inflammatory effect, the effect components are made into the liposome, so that the decomposition of oxidative free radicals in the environment to the plant pigment composition is inhibited, the natural plant pigment is protected, the plant pigment composition can be more stable in cosmetics, the lipid wrapping body can better penetrate through sebum membranes, the absorption of the effect components by skin cells is promoted, and the anti-inflammatory effect is effectively exerted.

The invention also aims to provide a preparation method of the essence composition of the liposome with anti-inflammatory effect, wherein the liposome is coated with the plant pigment.

The invention also aims to provide an application of the essence composition of the liposome with anti-inflammatory effect, wherein the liposome is coated with the phytochrome.

The technical scheme adopted by the invention is as follows.

The essence composition of the liposome with anti-inflammatory effect, which wraps the plant pigment, comprises the following raw material components by weight:

1.5-35.0% of anti-inflammatory efficacy liposome;

5.0-40.0% emollient;

0.1-30.0% of an emulsifier;

0.1-10.0% of a thickener;

0.5-20% skin conditioning agent;

0.1-10% of a pH regulator;

0.3-5.0% preservative;

0.01-1.0% of daily essence;

and the balance of deionized water.

Furthermore, the essence composition of the liposome with anti-inflammatory effect, which is coated with the plant pigment, comprises the following raw material components by the total weight of the composition:

further, the liposome with anti-inflammatory effect, which wraps the phytochrome, comprises the following raw material components:

0.1-10% of lycopene, 0.1-10% of beta-carotene, 0.1-10% of acetoshikonin, 0.1-10% of vitamin E,0.1-10% of vitamin C,0.1-10% of phosphorus ester and 0.1-10% of lecithin.

0.5-8% of lycopene, 0.5-8% of beta-carotene, 0.5-8% of acetoshikonin, 0.5-8% of vitamin E,0.3-10% of vitamin C,0.5-8% of phosphorus ester and 0.3-8% of lecithin.

0.5-5% of lycopene, 0.5-5% of beta-carotene, 0.5-5% of acetoshikonin, 0.5-5% of vitamin E,0.3-5% of vitamin C,1.0-6% of phosphorus ester and 0.3-5% of lecithin.

0.5-1.5% of lycopene, 0.5-2.5% of beta-carotene, 0.6-2.0% of acetoshikonin, 0.7-2.0% of vitamin E,0.3-1.0% of vitamin C,1.5-4.0% of phosphorus ester and 0.4-1.0% of lecithin.

Further, the emollient is selected from at least one of caprylic capric triglyceride, squalane, avocado oil, shea butter, jojoba oil, glycerin, panthenol, shea butter, guaiacol, tocopheryl acetate, butylene glycol, 1, 2-hexanediol, 4-t-butylcyclohexanol, pentylene glycol, cyclohexanol, trehalose, xylitol, allantoin, sodium hyaluronate, polydimethylsiloxane, dimethiconol, PEG-11 methyl ether dimethicone, phytosterol/octyldodecyl lauroyl glutamate, hydrogenated polyisobutylene, rapeseed oil, polydecene, soybean oil, olive oil, stearyl alcohol, behenyl alcohol.

The emulsifier is selected from at least one of olive oil PEG-7 esters, PEG-8 caprylic/capric glycerides, cholesterol, sorbitan olive oleate, polysorbate-20, triethanolamine, octyldodecanol, glyceryl tri (ethylhexanoate), triethoxyoctylsilane, C12-18 alkyl glucoside, laureth-23, a mixture of glyceryl stearate and PEG-100 stearate, steareth-21, palmitols and PEG-75 stearate and a mixture of glyceryl stearate and steareth-20, polysorbate-80, lecithin, phospholipids, a mixture of glyceryl stearate and PEG-75 stearate, poloxamer 407, PEG-30 dimer hydroxystearate, cetostearyl ether-25.

The thickener is at least one selected from hydroxyethyl cellulose, xanthan gum, cetyl alcohol, carbomer, acrylic acid (ester) copolymer, polymer EMT-10, polyacrylate crosslinked polymer-6, sodium acrylate/sodium acryloyldimethyl taurate copolymer, acrylic ester/C10-30 alkyl acrylate crosslinked polymer, acrylamide/VP copolymer, acrylic acid (ester) type/C10-30 alkanol acrylic ester crosslinked polymer, polyethylene and gum arabic.

The skin conditioner is at least one selected from herba Centellae extract, glycyrrhrizae radix extract and testa oryzae extract.

The pH regulator is at least one selected from citric acid, potassium hydroxide, lactic acid, sodium hydroxide, sodium citrate, sodium lactate, arginine and glutamic acid.

The preservative is at least one selected from the group consisting of p-hydroxyacetophenone, phenoxyethanol, ethylhexyl glycerol, hexanediol, benzoyl ketone, sodium benzoate, triclosan and chlorphenicol.

Further, the preparation method of the liposome with the anti-inflammatory effect and the plant pigment coating comprises the following process steps:

1) Dissolving phospholipid and lecithin in ethanol, and shaking for dissolving to obtain a membrane material solution;

2) Removing ethanol from the membrane material solution in the step 1) under reduced pressure at a constant temperature of 35 ℃ until a layer of transparent film is formed on the inner side of the bottle wall, and drying for 10min to obtain a dried product;

3) Dissolving lycopene, beta-carotene, acetoshikonin, vitamin E and vitamin C in propylene glycol water solution with concentration of 20% to obtain core material solution;

4) Adding the core material solution obtained in the step 3) into the dried product obtained in the step 2), and performing normal-pressure rotary oscillation hydration and demoulding at 35 ℃;

5) Ultrasonic treatment is carried out for 5-20min after the membrane removal is completed, and the anti-inflammatory liposome with uniform particle size is obtained after filtration.

Preferably, the mass ratio of lecithin to phospholipid is 1: (3-5); lycopene, beta-carotene, acetoshikonin, vitamin E and vitamin C in the mass ratio of 1: (1-2): (1-1.5): (1-2): (0.5-1); the mass ratio of lecithin to lycopene is 1: (1-1.5), wherein the volume ratio of the film material solution to the core material solution is 1: (2-5).

Specifically, the liposome particle size of the encapsulated plant pigment with anti-inflammatory effect is 0.1-0.5 μm.

In addition, the invention also discloses a preparation method of the essence composition of the liposome with anti-inflammatory effect, which comprises the following process steps:

1) Sequentially adding the raw materials of phase A into a main tank, stirring, mixing, dissolving uniformly, and heating to 80 ℃;

2) Then sequentially adding the phase B raw materials into the phase A, uniformly stirring, and homogenizing for 3 minutes at 3000 rpm;

3) Cooling to below 40 ℃, and then sequentially adding the C-phase raw materials; stirring for 10 minutes at 100 revolutions per minute until the components are uniformly mixed;

the phase A comprises the following raw material components: 0.1-10.0% of thickener, 5.0-40.0% of emollient, 0.3-5.0% of preservative;

the phase B comprises the following raw material components: 0.1-30.0% of an emulsifier;

the C phase comprises the following raw material components: 1.5-35.0% of anti-inflammatory liposome, 0.5-20% of skin conditioner, 0.1-10% of pH regulator and 0.01-1.0% of daily essence.

In particular, the phytochrome-encapsulated liposome concentrate composition of the present invention has a pH in the range of 4-10, preferably in the range of 5-8, and most preferably in the range of 5-7.

Specifically, the cosmetic composition of the liposome with anti-inflammatory effect, which is coated with phytochrome, can be in a solvent dispersion or suspension mode, can also be in the form of emulsified particles, or can be in the form of polymer coating.

Specifically, the cosmetic composition of the liposome with anti-inflammatory effect, which is coated with the phytochrome, can be also designed into other skin care product formulations, such as emulsion, face cream, eye cream, body milk, gel and sun-proof milk.

By means of the technical scheme, the invention has the following advantages and beneficial technical effects:

1) The plant pigment composition containing lycopene, beta-carotene and acetoshikonin various natural pigments has the advantages of high stability, difficult decomposition, low irritation and good anti-inflammatory and anti-allergic effects.

2) The anti-inflammatory functional component composition disclosed by the invention has the advantages that the liposome wrapping technology is used for wrapping two substances with strong reducibility, namely vitamin E and vitamin C, with the natural plant pigment composition, so that the decomposition of the plant pigment composition by oxidative free radicals in the environment is inhibited, the characteristic that the traditional pigment is easy to decompose or change color in the environment to lose effectiveness is overcome, the plant pigment composition can be widely applied to cosmetics, and the product quality is more stable, safe and effective.

3) The anti-inflammatory functional component is added into the cosmetic in a liposome form, so that the anti-inflammatory functional component can penetrate through the skin barrier more easily, and the transdermal absorption and slow release effects are better achieved.

4) The plant pigment composition of the invention can widely replace various mauve inorganic synthetic pigments in cosmetics, is safe and non-irritating, and has certain anti-inflammatory, antibacterial and antiallergic effects.

5) The novel anti-inflammatory effect cosmetic can effectively relieve the phenomenon of facial inflammation and allergy, and provides a plurality of ideal benefits for facial care.

Drawings

FIG. 1 shows the average pigment content of a sample stored at 40℃under high temperature;

FIG. 2 shows the average pigment content of a sample under light storage;

figure 3 shows the relevant improvement in inflammation in subjects before and after the use of the samples.

Detailed Description

The invention discloses an essence composition of liposome with anti-inflammatory effect and coated with phytochrome. The anti-inflammatory essence comprises the following raw material components:

1.5 to 35.0 percent of anti-inflammatory liposome, 5.0 to 40.0 percent of emollient, 0.1 to 30.0 percent of emulsifier, 0.1 to 10.0 percent of thickener, 0.5 to 20 percent of skin conditioner, 0.1 to 10 percent of pH regulator, 0.3 to 5.0 percent of preservative, 0.01 to 1.0 percent of daily essence and the balance of deionized water.

The liposome-encapsulated phytochrome composition with anti-inflammatory effect comprises 0.1-10% of lycopene, 0.1-10% of beta-carotene, 0.1-10% of acetoshikonin, 0.1-10% of vitamin E,0.1-10% of vitamin C,0.1-10% of phosphate and 0.1-10% of lecithin.

Lycopene is a straight-chain hydrocarbon compound composed of eleven conjugated double bonds and two non-conjugated double bonds, and is an intermediate for biosynthesis of various carotenoids. Lycopene is mainly present in tomato fruit of Solanaceae, is a natural functional pigment, has strong antioxidant effect, and can be widely used in medicine and cosmetics. According to related medical research, lycopene can regulate the immune system of human body and inhibit the damage of harmful substances in the environment to skin.

Beta-carotene is a fat-soluble carotenoid, has good oxidation resistance, anti-cancer and cancer prevention effects, has certain effects on enhancing organism immunity, restoring skin immune barrier, promoting regeneration of damaged skin and the like, and is a functional natural pigment with development value.

The acetyl shikonin is a natural plant pigment extracted from lithospermum plant, and compared with synthetic pigment, the acetyl shikonin does not poison human body, can protect skin, and has good skin affinity. It can be used in cosmetics to exhibit physiological activities such as anti-inflammatory and antiallergic effects. After the acetoshikonin acts on the surface of the skin, the skin is not stimulated, and a large amount of unsaturated fatty acid and hydrophilic groups in the acetoshikonin can be decomposed into the stratum corneum, so that necessary fatty acid is supplied to the skin, and the skin is protected.

The components with the anti-inflammatory effects penetrate through the surface barrier of the skin to the deep layer, supplement nutrition for the skin, remove free radicals, resist oxidation, ensure the skin immune barrier effect and play the anti-inflammatory and anti-allergic effects.

The invention is illustrated by the following examples which are given for further illustration of the invention and are not intended to limit the scope of the invention. Some insubstantial modifications and adaptations of the invention by others are within the scope of the invention. The following percentages (%) are mass percentages unless otherwise indicated. All raw material components of the invention are commercial products.

Examples 1 to 3 and comparative examples thereof

The contents of the raw material component ratios of the examples and the raw material component ratios of the comparative examples of the essence coating the plant pigment liposome with anti-inflammatory effect are shown in the following table 1.

TABLE 1 raw material composition ratios (unit: mass%) of examples 1 to 3 and comparative examples thereof

The preparation process flow of the essence composition embodiment of the liposome with anti-inflammatory effect is as follows:

3) Cooling to below 40 ℃, and then sequentially adding the C-phase raw materials. And then stirred for 10 minutes at 100 rpm. Stirring until the components are uniformly mixed.

The concentrate compositions with anti-inflammatory efficacy liposomes prepared in examples 1-3 have a pH in the range of 5-6.

The preparation method of the liposome with anti-inflammatory effect and coated with phytochrome of the embodiment 1 comprises the following process steps:

1) Dissolving 900mg of phospholipid and 240mg of lecithin in 30mL of ethanol, and shaking for dissolving until the solution is transparent to prepare a membrane material solution;

2) Removing ethanol from the membrane material solution in the step 1) under reduced pressure at a constant temperature of 35 ℃ until a layer of transparent film is formed on the inner side of the bottle wall, and drying for 10min;

3) 300mg of lycopene, 510mg of beta-carotene, 390mg of acetoshikonin, 450mg of vitamin E and 210mg of vitamin C are dissolved in 60mL of propylene glycol aqueous solution with the concentration of 20%, so as to prepare a core material solution;

5) Ultrasonic treatment is carried out for 5-20min after the membrane removal is completed, and liposome with anti-inflammatory effect, which is obtained by coating plant pigment with uniform particle size (0.22+/-0.05 mu m), is obtained after filtration.

The preparation method of the liposome with anti-inflammatory effect and coated with phytochrome of the embodiment 2 comprises the following process steps:

1) 1620mg of phospholipid and 432mg of lecithin are dissolved in 50mL of ethanol, and the solution is dissolved by shaking until the solution is transparent, so as to prepare a membrane material solution;

3) 540mg of lycopene, 918mg of beta-carotene, 702mg of acetoshikonin, 810mg of vitamin E and 378mg of vitamin C are dissolved in 110mL of propylene glycol aqueous solution with the concentration of 20%, so as to prepare a core material solution;

5) Ultrasonic treatment is carried out for 5-20min after the membrane removal is completed, and liposome with anti-inflammatory effect, which is obtained by coating plant pigment with uniform particle size (0.25+/-0.05 mu m), is obtained after filtration.

The preparation method of the liposome coated with phytochrome and having anti-inflammatory effect of the embodiment 3 comprises the following process steps:

1) Dissolving 2160mg of phospholipid and 576mg of lecithin in 80mL of ethanol, and shaking for dissolving until the solution is transparent to obtain a membrane material solution;

3) 720mg of lycopene, 1224mg of beta-carotene, 936mg of acetoshikonin, 1080mg of vitamin E and 504mg of vitamin C are dissolved in 200mL of propylene glycol aqueous solution with 20% concentration to prepare a core material solution;

5) Ultrasonic treatment is carried out for 5-20min after the membrane removal is completed, and liposome with anti-inflammatory effect, which is obtained by coating plant pigment with uniform particle size (0.20+/-0.05 mu m), is obtained after filtration.

Effect verification test example

Pigment stability Effect test 1

1. Test article: example 1 and comparative examples 1-1 and 1-2; example 2 and comparative examples 2-1 and 2-2; example 3 and comparative examples 3-1 and 3-2;

2. test environment: a high temperature storage environment at 40 ℃;

3. the testing method comprises the following steps: the essence of each of examples 1 to 3 and the control thereof was stored in a constant temperature incubator at 40℃and the average pigment content of the samples of examples and control was analyzed by measuring the plant pigment content of the samples before, during 1 month, 2 months, and 3 months by high performance liquid chromatography.

4. The test indexes are as follows: average pigment content (%) =average pigment content of sample at a certain time/average pigment content of sample before placement;

5. the test results are shown in fig. 1 and table 2 below:

TABLE 2 average pigment content of samples stored at 40℃high temperature

	Before placement	Standing for 1 month	Standing for 2 months	Standing for 3 months
					Example 1	100％	91％	83％	74％
Comparative example 1-1	100％	68％	45％	28％
					Comparative examples 1 to 2	100％	40％	26％	16％
Example 2	100％	93％	86％	81％
					Comparative example 2-1	100％	65％	48％	30％
Comparative example 2-2	100％	42％	31％	17％
					Example 3	100％	95％	85％	85％
Comparative example 3-1	100％	69％	51％	29％
					Comparative example 3-2	100％	42％	29％	19％

The results showed that the average pigment content after storage for one month at 40℃in examples 1 to 3 was 93.00%, the average pigment content after 2 months was 84.67%, and the average pigment content after 3 months was 80.00%.

The average pigment content after storage for one month in comparative examples 1 to 3-1 was 67.33%, the average pigment content after 2 months was 48.00%, and the average pigment content after 3 months was 29.00%.

The average pigment content after storage for one month in comparative examples 1 to 3-2 was 41.33%, the average pigment content after 2 months was 28.67%, and the average pigment content after 3 months was 17.33%.

Examples 1 to 3 are lipid inclusion bodies made of lycopene, beta-carotene, acetoshikonin, vitamin E and vitamin C, and the isolation of the lipid film from the oxygen environment and the presence of antioxidant vitamins can effectively protect the stability of pigments in products. Examples 1 to 3-1 were lycopene, beta-carotene, acetoshikonin, vitamin E and vitamin C were added directly to the product, and the protective effect was limited in spite of the presence of antioxidants. Examples 1-3-2 are lycopene, beta-carotene and acetoshikonin which are directly added into the product, and the color fading is serious after high-temperature storage.

Pigment stabilization Effect test 2

2. test environment: simulating an illumination storage environment;

3. the testing method comprises the following steps: the essence of each of examples 1-3 and the control thereof were placed in a GZP-250 light incubator to simulate sunlight irradiation environment for storage, and the content of phytochrome in the samples before, during 1 month, 2 months and 3 months was measured by high performance liquid chromatography, respectively, to analyze the change of the average pigment content of the samples of examples 1-3 and the control thereof.

5. the test results are shown in fig. 2 and table 3 below:

TABLE 3 average pigment content of light storage samples

	Before placement	Standing for 1 month	Standing for 2 months	Standing for 3 months
					Example 1	100％	90％	79％	69％
Comparative example 1-1	100％	58％	39％	25％
					Comparative examples 1 to 2	100％	38％	23％	12％
Example 2	100％	92％	77％	70％
					Comparative example 2-1	100％	64％	38％	26％
Comparative example 2-2	100％	40％	21％	15％
					Example 3	100％	91％	81％	74％
Comparative example 3-1	100％	62％	44％	28％
					Comparative example 3-2	100％	41％	22％	14％

The results showed that the average pigment content of examples 1 to 3 after one month of storage in the simulated light storage environment was 91.00%, the average pigment content after 2 months of storage was 79.00%, and the average pigment content after 3 months of storage was 71.00%.

The average pigment content after storage for one month was 61.33% for comparative examples 1 to 3-1, the average pigment content after 2 months was 40.33%, and the average pigment content after 3 months was 26.33%.

The average pigment content after storage for one month was 39.67% for comparative examples 1 to 3-2, the average pigment content after 2 months was 22.00%, and the average pigment content after 3 months was 13.67%.

Examples 1 to 3 are lipid inclusion bodies made of lycopene, beta-carotene, acetoshikonin, vitamin E and vitamin C, examples 1 to 3-1 are lycopene, beta-carotene, acetoshikonin, vitamin E and vitamin C directly added into products, and examples 1 to 3-2 are lycopene, beta-carotene and acetoshikonin directly added into products. The dual protection of the lipid film and the antioxidant against the pigment in the examples results in a much higher pigment content of the product during storage than in the control examples.

In summary, in the embodiment of the invention, the natural plant pigment, vitamin E and vitamin C are coated together to prepare the lipid inclusion body, and compared with the direct addition of the plant pigment, the embodiment of the invention can effectively protect the stability of the natural pigment in the product.

Anti-inflammatory Effect test 3

The contents of the raw material component ratios of comparative examples 4 to 11, which are compared with the raw material component ratio of example 2, are shown in the following table 4.

TABLE 4 raw material composition ratio (unit: mass%) of comparative example

The preparation process flow is as follows:

1. The tester: 90 healthy persons (20 to 55 years old), women were randomly divided into 9 groups, and example 2 and comparative examples 4 to 11 were used respectively; the face has red spots, itching, rash and other related inflammatory conditions;

2. test part: full face;

3. test instrument: the Visia-CR is used for facial image acquisition;

4. evaluation criteria: the dermatologist performs an inflammation improvement effect evaluation according to the face of the subject and the acquired image thereof: the ratio of the number of people is counted in two categories, namely slight improvement and obvious improvement.

5. Test results: as shown in table 5 and fig. 3.

TABLE 5 improvement of subject related inflammatory conditions

The results showed that the effect of improving various inflammations such as erythema, itching, and rash was remarkable after using example 2. Compared with the comparison example of directly adding the natural plant pigment composition into the formula or adding the natural pigment composition into the formula singly or in two types, the anti-inflammatory effect of the embodiment of the invention is better. And the improvement effect on skin inflammation is more remarkable than that of the synthetic inorganic pigment in the comparative example 5.

Security verification 4

1. Number of test persons: 30 healthy persons (20-55 years old) were randomized into 3 groups and tested with the formulations of examples 1-3, respectively.

2. Test part: forearm curved side;

3. the testing method comprises the following steps: the selected area is not more than 50mm ² A suitable plaque tester having a depth of about 1mm is provided with a test object of about 0.020 to 0.025mL (g) by a closed plaque test method. The patch tester was applied to the forearm of the subject, the test substance was removed after 24 hours, and skin reactions were observed at 0.5, 24, and 48 hours after the removal, and the results were recorded according to the skin reaction classification standard in cosmetic safety technical Specification 2015.

4. The evaluation criteria are shown in Table 6 below:

TABLE 6 skin reaction grading criteria

The results show that: the results of the human skin patch test showed that the score levels of 3 observation time points 0.5h, 24h and 48h after patch removal were all 0 points for 30 subjects. According to the specification of cosmetic safety technology (2015 edition), the test object does not cause adverse skin reaction to the subjects in the batch.

The essence composition of the liposome with anti-inflammatory effect, which is wrapped by plant pigment, consists of three natural plant pigments of lycopene, beta-carotene and acetoshikonin, has strong antioxidation, effectively eliminates free radicals, promotes the regeneration of damaged skin, reduces the production of inflammatory factors and restores the skin immune barrier. The natural pigment lipid inclusion has good skin affinity, is safer than the synthetic pigment, has good anti-inflammatory and antiallergic effects when applied to cosmetics, and can widely replace various mauve inorganic synthetic pigments in cosmetics.

The above multiple functional components are synergistic, and the liposome form can make the active components act through the skin surface layer to enhance absorption, thereby achieving effective anti-inflammatory effect.

In conclusion, the anti-inflammatory efficacy compound has obvious anti-inflammatory and anti-allergic effects through the synergistic effect of the components, and the efficacy liposome has more obvious anti-inflammatory effects than the efficacy composition and the combination of two or single components.

The foregoing description is only a preferred embodiment of the present invention and is not intended to limit the invention in any way, so any simple modification, equivalent variation and modification made to the above embodiment according to the technical matter of the present invention will still fall within the scope of the technical scheme of the present invention.

Claims

1. An essence composition of liposome with anti-inflammatory effect is characterized in that: based on the total weight of the composition, the composition consists of the following raw materials:

1.5-35.0% of anti-inflammatory efficacy liposome;

5.0-40.0% emollient;

0.1-30.0% of an emulsifier;

0.1-10.0% of a thickener;

0.5-20% skin conditioning agent;

0.1-10% of a pH regulator;

0.3-5.0% preservative;

0.01-1.0% of daily essence and the balance of deionized water;

the anti-inflammatory efficacy liposome comprises: 0.1-10% of lycopene, 0.1-10% of beta-carotene, 0.1-10% of acetoshikonin, 0.1-10% of vitamin E,0.1-10% of vitamin C,0.1-10% of phosphoester and 0.1-10% of lecithin;

the mass ratio of the lycopene, the beta-carotene, the acetoshikonin, the vitamin E and the vitamin C is 1: (1-2): (1-1.5): (1-2): (0.5-1).

2. The concentrate composition of claim 1, wherein the liposome has anti-inflammatory efficacy, wherein: based on the total weight of the composition, the composition consists of the following raw materials:

2.0-25.0% of anti-inflammatory liposome;

10.0 to 25.0 percent of emollient;

0.1-20.0% of emulsifying agent;

0.2-5.0% of thickener;

1.0-15% of skin conditioning agent;

0.5-5% of pH regulator;

0.3-1.0% of preservative;

0.1-0.5% of daily essence;

the balance of deionized water was added to 100%.

3. The concentrate composition of claim 1, wherein the liposome has anti-inflammatory efficacy, wherein: the anti-inflammatory efficacy liposome comprises, based on the total weight of the composition:

4. The concentrate composition of claim 1, wherein the liposome has anti-inflammatory efficacy, wherein: the anti-inflammatory efficacy liposome comprises, based on the total weight of the composition:

5. The concentrate composition of liposomes having anti-inflammatory efficacy according to any one of claims 1 to 4, wherein: the anti-inflammatory liposome is prepared by the following process:

2) Removing ethanol from the film material solution in the step 1) under reduced pressure at a constant temperature of 35 ℃ until a layer of transparent film is formed on the inner side of the bottle wall, and drying for 10min to obtain a dried product;

6. The concentrate composition of claim 5, wherein the liposome has anti-inflammatory efficacy, and is characterized by:

the mass ratio of the lecithin to the phospholipid is 1: (3-5);

the mass ratio of the lecithin to the lycopene is 1: (1-1.5);

the volume ratio of the film material solution to the core material solution is 1: (2-5);

the particle size of the obtained liposome with anti-inflammatory effect is 0.1-0.5 μm.

7. The concentrate composition of liposomes having anti-inflammatory efficacy according to any one of claims 1 to 4, wherein:

the emollient is selected from at least one of caprylic capric triglyceride, squalane, avocado oil, shea butter, jojoba oil, glycerol, panthenol, shea butter, guaiacol, tocopheryl acetate, butylene glycol, 1, 2-hexanediol, 4-t-butylcyclohexanol, pentylene glycol, cyclohexanol, trehalose, xylitol, allantoin, sodium hyaluronate, polydimethylsiloxane, dimethiconol, PEG-11 methyl ether dimethicone, phytosterol, hydrogenated polyisobutene, rapeseed oil, polydecene, soybean oil, olive oil, stearyl alcohol, and behenyl alcohol;

the emulsifier is selected from at least one of olive oil PEG-7 esters, PEG-8 caprylic/capric glycerides, cholesterol, sorbitan olive oleate, polysorbate-20, triethanolamine, octyldodecanol, glycerol tri (ethylhexanoate), C12-18 alkyl glucoside, laureth-23, a mixture of glycerol stearate and PEG-100 stearate, steareth-21, palmitols and a mixture of PEG-75 stearate and glycerol stearate and steareth-20, polysorbate-80, a mixture of glyceryl stearate and PEG-75 stearate, poloxamer 407, PEG-30 dimer hydroxystearate, cetostearyl polyether-25;

the thickener is at least one selected from hydroxyethyl cellulose, xanthan gum, cetyl alcohol, carbomer, acrylic acid (ester) copolymer, polymer EMT-10, polyacrylate crosslinked polymer-6, sodium acrylate/sodium acryloyldimethyl taurate copolymer, acrylic ester/C10-30 alkyl acrylate crosslinked polymer, acryloyldimethyl taurate amine/VP copolymer, acrylic acid (ester) type/C10-30 alkanol acrylic ester crosslinked polymer, polyethylene and gum arabic;

the skin conditioner is at least one selected from centella asiatica extract, licorice extract and rice chaff extract;

the pH regulator is at least one selected from citric acid, potassium hydroxide, lactic acid, sodium hydroxide, sodium citrate, sodium lactate, arginine and glutamic acid;

8. The method for preparing the essence composition with anti-inflammatory efficacy liposome according to any one of claims 1 to 4, which is characterized by comprising the following process steps:

9. A cosmetic product characterized by: the cosmetic comprises the essence composition of the liposome with anti-inflammatory effect prepared by the preparation method of claim 8.