CN114099410B

CN114099410B - Face cream composition of liposome with synergistic gain and anti-aging effects and preparation method thereof

Info

Publication number: CN114099410B
Application number: CN202111397927.XA
Authority: CN
Inventors: 王勇; 周紫燕; 杨帆; 祝希宇; 郭苗; 周正
Original assignee: Hubei Maishite Biotechnology Co Ltd
Current assignee: Hubei Maishite Biotechnology Co Ltd
Priority date: 2021-11-23
Filing date: 2021-11-23
Publication date: 2023-06-23
Anticipated expiration: 2041-11-23
Also published as: CN114099410A

Abstract

The invention discloses a facial cream composition of liposome with synergistic gain and anti-aging effects and a preparation method thereof. The anti-aging facial cream composition comprises the following raw material components: 0.3-35.0% of synergistic gain anti-aging efficacy liposome, 5.0-40.0% of emollient, 0.5-30.0% of emulsifier, 0.5-20.0% of filler, 0.1-10.0% of thickener, 0.3-5.0% of preservative, 0.01-1.0% of daily essence and the balance of deionized water. The anti-aging functional component consists of 0.1-30% of bacillus licheniformis/glutamic acid fermentation filtrate, 0.1-30% of bird's nest acid and 0.1-30% of procyanidine, and is prepared into a lipid inclusion, so that on one hand, the activity of the functional component in a formula is protected, on the other hand, the lipid inclusion can better penetrate through a sebum membrane, the absorption of skin cells to the functional component is promoted, and the anti-aging effect is effectively exerted.

Description

Face cream composition of liposome with synergistic gain and anti-aging effects and preparation method thereof

Technical Field

The invention relates to a cosmetic composition and a preparation method thereof, belongs to the technical field of daily chemicals, and in particular relates to a facial cream composition containing lipid inclusion with synergistic gain anti-aging effects of various anti-aging components and a preparation method thereof.

Background

The skin of the human body generally ages gradually with age from 25 to 30 years old, and gradually develops more obvious aging changes after about 35 to 40 years old. Skin aging is a complex, multi-factor, complex process that can be categorized into endogenous aging and exogenous aging according to the cause of aging.

Wherein endogenous aging is also known as natural aging, is mainly related to the following factors:

(1) the permeability of the stratum corneum is increased, the barrier function of the skin is reduced, the moisture content in the stratum corneum is reduced, and the skin is in a water-deficient state;

(2) hypofunction of skin accessory organs such as sweat glands and sebaceous glands gradually decrease with age, resulting in reduced amounts of sweat and sebum secreted, and skin is not nourished for a long period of time and is dried;

(3) the metabolism speed of the skin is reduced, so that the functions of elastic fibers and collagen fibers in the dermis are reduced, the regulating effect of the tension and the elasticity of the skin is weakened, and the skin is easy to wrinkle;

(4) insufficient nutrition is absorbed by the skin, so that subcutaneous fat storage is continuously reduced, and the lower part of the dermis reticular layer is unsupported, so that the skin is loose.

Exogenous aging refers to skin aging caused by external factors such as ultraviolet radiation, smoking, wind and sun exposure, and exposure to toxic and harmful chemicals, and the damage of ultraviolet rays in sunlight to skin is various, and most importantly, causes changes in the components of intradermal fibrin and matrix, which ultimately results in dryness, relaxation and inelasticity of skin.

Aging is a natural law of human growth and cannot be prevented artificially, but the skin aging causes can be analyzed to pointedly supplement moisture and nutrition to the skin and resist external injury, so that the aging is effectively delayed, and the skin is in a young and healthy skin state.

However, the existing anti-aging products on the market have various defects of unobvious effect, long effect period, poor permeability and the like, so that the face cream skin care product which is easier to absorb, better in anti-aging effect and safer is provided, and the technical problem which is urgently needed to be solved by the person skilled in the art at present.

Disclosure of Invention

The invention aims to provide a facial cream composition containing various anti-aging efficacy components and liposome with anti-aging efficacy, which can synergistically increase the anti-aging effect, and solves the problems in the background technology.

Another object of the present invention is to provide a method for preparing a cream composition having synergistic anti-aging efficacy liposomes.

The technical scheme adopted by the invention is as follows.

The face cream composition with the liposome with the synergistic gain and anti-aging effects comprises the following raw material components by weight:

0.3-35.0% of anti-aging efficacy liposome;

5.0-40.0% emollient;

0.5-30.0% of an emulsifier;

0.5-20.0% filler;

0.1-10.0% of a thickener;

0.3-5.0% preservative;

0.01-1.0% of daily essence;

and the balance of deionized water.

Furthermore, the face cream composition with the liposome with the synergistic gain and anti-aging effects comprises the following components in terms of the total weight of the composition:

further, the anti-aging efficacy liposome comprises the following raw material components:

bacillus licheniformis/glutamic acid fermentation filtrate 0.1-30%, nidus Collocaliae acid 0.1-30% and procyanidine 0.1-30%.

bacillus licheniformis/glutamic acid fermentation filtrate 0.1-10%, nidus Collocaliae acid 0.1-10% and procyanidine 0.1-10%.

bacillus licheniformis/glutamic acid fermentation filtrate 0.5-10%, nidus Collocaliae acid 0.5-10% and procyanidine 0.5-10%

bacillus licheniformis/glutamic acid fermentation filtrate 0.5-5%, nidus Collocaliae acid 0.5-5% and procyanidine 0.5-5%.

the anti-aging efficacy liposome comprises the following raw material components:

5-20% of bacillus licheniformis/glutamic acid fermentation filtrate, 5-25% of nidus Collocaliae acid and 4-15% of procyanidine.

Further, the emollient is selected from at least one of caprylic capric triglyceride, squalane, avocado oil, shea butter, jojoba oil, glycerin, panthenol, shea butter, guaiacol, tocopheryl acetate, butylene glycol, 1, 2-hexanediol, 4-t-butylcyclohexanol, pentylene glycol, cyclohexanol, trehalose, xylitol, sodium hyaluronate, polydimethylsiloxane, dimethiconol, PEG-11 methyl ether dimethicone, phytosterol/octyldodecyl lauroyl glutamate, hydrogenated polyisobutylene, rapeseed oil, polydecene, soybean oil, olive oil, lactic acid, arginine, stearyl alcohol, and behenyl alcohol.

The emulsifier is selected from at least one of olive oil PEG-7 esters, PEG-8 caprylic/capric glycerides, cholesterol, sorbitan olive oleate, polysorbate-20, triethanolamine, octyldodecanol, glyceryl tri (ethylhexanoate), triethoxyoctylsilane, C12-18 alkyl glucoside, laureth-23, a mixture of glyceryl stearate and PEG-100 stearate, steareth-21, palmitols and PEG-75 stearate and a mixture of glyceryl stearate and steareth-20, polysorbate-80, lecithin, phospholipids, a mixture of glyceryl stearate and PEG-75 stearate, poloxamer 407, PEG-30 dimer hydroxystearate, cetostearyl ether-25. The filler is at least one selected from titanium dioxide, mica, silica, boron nitride, maltodextrin, polymethylsilsesquioxane, diatomite, kaolin, cellulose acetate, starch octyl aluminum succinate, talcum powder, barium sulfate and volcanic ash. The thickener is at least one selected from hydroxyethyl cellulose, xanthan gum, cetyl alcohol, carbomer, acrylic acid (ester) copolymer, polymer EMT-10, polyacrylate crosslinked polymer-6, sodium acrylate/sodium acryloyldimethyl taurate copolymer, acrylic ester/C10-30 alkyl acrylate crosslinked polymer, acrylamide/VP copolymer, acrylic acid (ester) type/C10-30 alkanol acrylic ester crosslinked polymer, polyethylene and gum arabic. The preservative is at least one selected from the group consisting of p-hydroxyacetophenone, phenoxyethanol, ethylhexyl glycerol, hexanediol, benzoyl ketone, sodium benzoate, triclosan and chlorphenicol.

Further, the preparation method of the liposome with the synergistic gain anti-aging effect comprises the following process steps:

1) Dissolving phospholipid and lecithin in ethanol, and shaking to dissolve until the solution is transparent to obtain an oil phase membrane material solution;

2) Removing ethanol from the film material solution in the step 1) under reduced pressure at a constant temperature of 35 ℃ until a layer of transparent film is formed on the inner side of the bottle wall, and drying for 10min to obtain a dried product;

3) Dissolving bacillus licheniformis/glutamic acid fermentation filtrate, nidus Collocaliae acid and procyanidine in phosphate buffer solution with pH of 6.8 to obtain water phase active phosphate buffer solution;

4) Adding the active phosphate buffer solution in the step 3) into the dried product obtained in the step 2), and performing normal-pressure rotary oscillation hydration and demoulding at 35 ℃;

5) Ultrasonic treatment is carried out for 5-20min after the membrane is removed completely, and the anti-aging functional component liposome with uniform particle size (0.1-0.5 μm) is obtained after filtration.

Preferably, the mass ratio of phospholipid to lecithin is 1:1, a step of; the mass ratio of the bacillus licheniformis/glutamic acid fermentation filtrate to procyanidine to the nidus Collocaliae acid to lecithin is (0.5-8.0): 1: (0.5-6.0): (1-15); the volume ratio of the water phase to the oil phase is 1: (2-5).

Specifically, the particle size of the liposome with synergistic gain anti-aging effect is 0.1-0.5 mu m.

In addition, the invention also discloses a preparation method of the face cream composition with the liposome with the synergistic gain anti-aging effect, which comprises the following process steps:

1) Sequentially adding the raw materials of phase A into a main tank, stirring, mixing, dissolving uniformly, and heating to 80 ℃;

2) Sequentially adding each oil of phase B into an oil phase pot, heating to 80 ℃ until each substance is completely dissolved, adding phase B into phase A, emulsifying and homogenizing, and homogenizing for 3 minutes at 3000 rpm;

3) Sequentially adding the C-phase raw materials, homogenizing at 3000 rpm for 3 min, and stirring and cooling to 40 ℃;

4) Sequentially adding the phase D raw materials, and uniformly stirring;

the raw material composition of the face cream composition comprises:

phase A raw materials: 0.1-10.0% of a thickener; 5.0-20.0% emollient; 0.3-5.0% preservative; deionized water;

phase B raw materials: 0-10.0% emollient; 0.4-20% of an emulsifier;

c phase raw materials: 0.1-10% of an emulsifier;

the phase D is composed of 0.3-35.0% of anti-aging liposome, 0.01-1.0% of daily essence, 0.5-20.0% of filler, and 0-10.0% of emollient.

In particular, the present invention provides a cream composition having synergistically enhanced anti-aging efficacy liposomes having a pH in the range of 4-10, preferably a pH in the range of 5-8, most preferably a pH in the range of 5-7.

Specifically, the cream composition of the synergistic anti-aging efficacy liposome can be in a solvent dispersion or suspension mode, can be in the form of emulsified particles or can be in the form of polymer encapsulation.

Specifically, the facial cream composition of the synergistic anti-aging liposome can be designed into other skin care product formulations, such as emulsion, essence, facial cream, eye cream, body milk, gel and sun-proof milk.

By means of the technical scheme, the invention has the following advantages and beneficial technical effects:

1) The invention contains bacillus licheniformis/glutamic acid fermentation filtrate, procyanidine and bird's nest acid, and is rich in nutrition, moisturizing, anti-wrinkle and anti-radiation, and the like.

2) The anti-aging functional component composition is preferably bacillus licheniformis/glutamic acid fermentation filtrate, procyanidine and bird's nest acid, and cosmetics can play an anti-aging role by selecting the most preferable composition proportion through preferable combination and efficacy test.

3) The anti-aging functional component composition is added into cosmetics in a liposome form, and the water-soluble functional component is wrapped in a lipid membrane, so that the anti-aging functional component penetrates through a skin barrier, and the effects of percutaneous absorption and slow release are better achieved.

4) On one hand, the invention utilizes the water supplementing capability of the efficacy composition, enhances the water retention capacity of skin, improves dry skin, reduces fine lines of skin caused by water deficiency, and delays skin aging; on the other hand, the composition is used for supplementing nutrition to the skin, promoting the synthesis of collagen, inhibiting elastase, assisting the body to protect the collagen and improving the elasticity of the skin; improving the healthy circulation of the skin to avoid or reduce the occurrence of wrinkles. In addition, the liposome component promotes the active substances to permeate the skin cuticle, so as to achieve the optimal action and effect, and further achieve the aim of comprehensively resisting aging.

5) Compared with most of cosmetics sold in the market at present, the liposome face cream with anti-aging efficacy provided by the embodiment of the invention has the advantages that the added efficacy components are mutually cooperated, the safe and effective anti-aging efficacy is achieved, and the obvious synergic effect is achieved. The face cream is suitable for more people with different skin types, and has wide application range and obvious anti-aging effect.

6) The innovative anti-aging effect cosmetic can alleviate the phenomenon of facial fine lines, and provides a plurality of ideal benefits for facial care so as to bring younger appearance.

Drawings

FIG. 1 shows the rate of change of skin wrinkle area before and after use of example 1 and blank;

FIG. 2 shows the rate of change of the Ra value of skin wrinkles before and after the use of example 1 and blank;

FIG. 3 shows the rate of change of clinical scores for skin wrinkles before and after use of example 1 and blank;

FIG. 4 shows the Ra value change rates of skin wrinkles before and after using example 1 and comparative examples 1 to 7.

Detailed Description

The invention discloses a facial cream composition of liposome with synergistic gain and anti-aging effects and a preparation method thereof. The anti-aging effect face cream comprises the following raw material components:

0.3-35.0% of anti-aging liposome, 5.0-40.0% of emollient, 0.5-30.0% of emulsifier, 0.5-20.0% of filler, 0.1-10.0% of thickener, 0.3-5.0% of preservative, 0.01-1.0% of daily essence and the balance of deionized water.

The anti-aging functional component is composed of functional liposome prepared from bacillus licheniformis/glutamic acid fermentation filtrate, nidus Collocaliae acid and procyanidine.

The bacillus licheniformis/glutamic acid fermentation filtrate is rich in amino acid, namely important components of natural moisturizing factors, can enhance the content of the natural moisturizing factors in skin, immediately supplement water, enhance the skin water retention capacity, improve dry skin and make the skin extremely moist; the product is rich in organic acid, can maintain the acidic environment of skin, can smooth horny layer, promote metabolism and maintain healthy skin; contains polysaccharide prebiotic component, helps the growth of beneficial bacteria, effectively inhibits harmful bacteria, and maintains the microecological balance of skin.

The nidus Collocaliae acid is one of eight nutrients essential to human body, and mainly contains nidus Collocaliae, breast milk, etc. The bird's nest is rich in content, so the bird's nest is called as bird's nest acid. The bird's nest acid is widely distributed in human tissues, exists in the forms of cell membrane glycoprotein, glycolipid and the like, has various biological functions, and has the effects of brightening skin, resisting aging, resisting oxidation, resisting pigmentation and the like.

Procyanidins are a general term for a large class of polyphenols widely found in plants, found in the skin, shell, seed, core, flowers, leaves of many plants. The procyanidine has strong antioxidant and free radical eliminating effects, can effectively eliminate superoxide anion free radical and hydroxyl free radical, also can protect lipid from peroxidation damage, is a powerful metal chelating agent, can chelate metal ions, and is also beneficial to absorption and utilization of vitamin C. Can be used in cosmetics for eliminating edema; helping collagen fibers form a cross-linked structure, recovering the activity of collagen, and enabling skin to be smooth and elastic; sun protection and whitening; astringing and moisturizing; radiation resistance and multiple effects.

The components with the anti-aging effects are mutually synergistic, and various active components penetrate through the surface barrier of the skin to the deep layer, so that the skin is supplemented with moisture and nutrition, the activity of collagen is enhanced, and the anti-aging effect of resisting wrinkles is exerted.

The invention is illustrated by the following examples which are given for further illustration of the invention and are not intended to limit the scope of the invention. Some insubstantial modifications and adaptations of the invention by others are within the scope of the invention. The following percentages (%) are mass percentages unless otherwise indicated. The materials used in the invention are all commercial products.

Examples 1 to 4 and blank examples

A facial cream composition with synergistic gain and antiaging effects comprises the following raw materials in proportion and blank raw materials in proportion as shown in table 1.

TABLE 1 raw material composition ratios (unit: mass%) of examples 1 to 4 and blank examples

The preparation process flow of the facial cream composition with the liposome with the synergistic gain anti-aging effect is as follows:

4) Sequentially adding the phase D raw materials, and stirring uniformly.

The cream compositions prepared in examples 1-4 with synergistic anti-aging efficacy liposomes have a pH in the range of 5-6.

The preparation method of the liposome in the cream composition containing the synergistic anti-aging efficacy liposome of the embodiment 1 comprises the following process steps:

1) Dissolving 1500mg of phospholipid and 1500mg of lecithin in 100mL of ethanol, and shaking for dissolving until the solution is transparent to prepare an oil phase membrane material solution;

2) Removing ethanol from the membrane material solution in the step 1) under reduced pressure at a constant temperature of 35 ℃ until a layer of transparent film is formed on the inner side of the bottle wall, and drying for 10min;

3) Dissolving 1500mg of nidus Collocaliae acid, 300mg of procyanidine and 1200mg of Bacillus licheniformis/glutamic acid fermentation filtrate in 30mL of phosphate buffer solution with pH of 6.8 to obtain water phase active phosphate buffer solution;

5) Ultrasonic treatment is carried out for 5-20min after the membrane is removed completely, and liposome with anti-aging effect with uniform particle size (0.18+/-0.03 mu m) is obtained after filtration.

The preparation method of the liposome in the cream composition containing the synergistic anti-aging efficacy liposome of the embodiment 2 comprises the following process steps:

1) Dissolving 2700mg of phospholipid and 2700mg of lecithin in 120mL of ethanol, and shaking for dissolving until the solution is transparent to prepare an oil phase membrane material solution;

3) Dissolving 1200mg of cubilose acid, 1050mg of procyanidine and 750mg of bacillus licheniformis/glutamic acid fermentation filtrate in 30mL of phosphate buffer solution with pH of 6.8 to prepare an aqueous phase active phosphate buffer solution;

5) Ultrasonic treatment is carried out for 5-20min after the membrane is removed completely, and liposome with anti-aging effect with uniform particle size (0.16+/-0.05 μm) is obtained after filtration.

The preparation method of the liposome in the cream composition containing the synergistic anti-aging efficacy liposome of the embodiment 3 comprises the following process steps:

1) Dissolving 3300mg of phospholipid and 3300mg of lecithin in 120mL of ethanol, and shaking for dissolving until the solution is transparent to prepare an oil phase membrane material solution;

3) 750mg of cubilose acid, 450mg of procyanidine and 1800mg of bacillus licheniformis/glutamic acid fermentation filtrate are dissolved in 30mL of phosphate buffer solution with pH of 6.8 to prepare an aqueous phase active phosphate buffer solution;

5) Ultrasonic treatment is carried out for 5-20min after the membrane is removed completely, and liposome with anti-aging effect with uniform particle size (0.15+/-0.05 μm) is obtained after filtration.

The preparation method of the liposome in the cream composition containing the synergistic anti-aging efficacy liposome of the embodiment 4 comprises the following process steps:

1) Dissolving 3900mg of phospholipid and 3900mg of lecithin in 150mL of ethanol, and shaking for dissolving until the solution is transparent to prepare an oil phase membrane material solution;

3) 1350mg of cubilose acid, 750mg of procyanidine and 900mg of bacillus licheniformis/glutamic acid fermentation filtrate are dissolved in 30mL of phosphate buffer solution with pH of 6.8 to prepare aqueous phase active phosphate buffer solution;

5) Ultrasonic treatment is carried out for 5-20min after the membrane is removed completely, and liposome with anti-aging effect with uniform particle size (0.13+/-0.05 μm) is obtained after filtration.

Effect verification test example

Test 1 of anti-aging and wrinkle-reducing Effect

1. The tester: 60 healthy people (20-55 years old), women, randomly divided into two groups, one group using example 1 and the other group using blank examples; the face has no obvious redness, skin damage, scars and the like;

2. test part: full face;

3. test instrument: visia-CR is used for facial image acquisition and wrinkle analysis. Primos is used for facial image acquisition and wrinkle analysis.

4. The test indexes are as follows: skin wrinkle area change rate, skin wrinkle Ra value change rate; rate of change = (post-use measurement-pre-use measurement)/(pre-use measurement x 100%;

5. the test results are shown in the following FIGS. 1-2 and tables 2-3:

TABLE 2 comparison of skin wrinkle area Rate before and after

	D0	D7	D14	D28	D56
						Example 1	2.44	2.39	2.10	1.86	1.62
Blank examples	2.38	2.38	2.35	2.31	2.30

TABLE 3 skin wrinkle Ra value

	D0	D7	△D14	△D28	△D56
						Example 1	23.86	22.61	20.79	19.33	18.24
Blank examples	23.75	23.67	23.61	23.56	23.58

Skin wrinkles facial image acquisition and wrinkle analysis were performed using Visia-CR and Primos. The area ratio of skin wrinkles measured according to Visia-CR at five time points D0, D7, D14, D28, and D56 (Table 2) showed that the area of skin wrinkles was reduced by 2.05% after 7 days of use after use of example 1 compared to before use; after 14 days of use, the area of skin wrinkles is reduced by 13.93%; after 28 days of use, the wrinkle area is reduced by 23.77%; after 56 days of use, the area of wrinkles is reduced by 33.61%. Compared with the results of the blank examples, the change rate of the area rate of the wrinkles in the example 1 is remarkably different after 7 days of use; after 14 days of use, the rate of change of the area rate of wrinkles in example 1 was significantly different; after 28 days of use, the rate of change of the area rate of wrinkles in example 1 was significantly different; after 56 days of use, the rate of change of the area rate of wrinkles in example 1 was significantly different.

Skin wrinkle Ra values (table 3) at five time points D0, D7, D14, D28, D56 measured according to Primos, and the results show that the skin wrinkle Ra value after use of example 1 was reduced by 5.24% after use for 7 days compared to before use; after 14 days of use, the Ra value of skin wrinkles decreased by 12.87%; after 28 days of use, the wrinkle Ra value decreased by 18.99%; after 56 days of use, the wrinkle Ra value decreased by 23.55%. Compared with the results of the blank examples, the Ra value change rate of the wrinkles of the example 1 is significantly different after 7 days of use; after 14 days of use, the Ra value change rate of the wrinkles of example 1 was significantly different; after 28 days of use, the Ra value change rate of the wrinkles of example 1 was significantly different; the Ra value change rate of example 1 was significantly different after 56 days of use.

In conclusion, the embodiment of the invention contains the anti-aging efficacy liposome, and has obvious anti-wrinkle and anti-aging effects.

Dermatological doctor clinical evaluation trial 2

2. test part: full face;

4. Evaluation criteria: the dermatologist performs wrinkle assessment from the acquired images: a score of 0 indicates no wrinkles and a score of 6 indicates very deep wrinkles.

5. Test results: as shown in table 4 and fig. 3.

Table 4 mean of clinical scores for wrinkles in two groups of subjects

	D0	D7	D14	D28	D56
						Example 1	2.34	2.21	2.07	1.88	1.69
Blank examples	2.28	2.28	2.25	2.23	2.21

The results showed that the clinical score of skin wrinkles was reduced by 5.56% after 7 days of use compared to before use in example 1; after 14 days of use, the clinical score of skin wrinkles is reduced by 11.54%; after 28 days of use, the clinical score of wrinkles is reduced by 19.66%; after 56 days of use, the wrinkle clinical score was reduced by 27.78%. Compared with the results of blank cases, the change rate of the clinical score of the wrinkle of the example 1 is significantly different after 7 days of use; after 14 days of use, the rate of change of the clinical score of the wrinkles in example 1 is significantly different; after 28 days of use, the rate of change of the clinical score of the wrinkles in example 1 was significantly different; after 56 days of use, example 1 showed a significant difference in the rate of change of the clinical score of wrinkles.

In conclusion, the embodiment of the invention has obvious anti-wrinkle effect, and wrinkles are one of the characterization of skin aging, so that the embodiment of the invention can effectively delay skin aging.

Security verification 3

1. Number of test persons: 30 healthy persons (20-55 years old), test case sample 1 formula.

2. Test part: forearm curved side;

3. the testing method comprises the following steps: the selected area is not more than 50mm ² A suitable plaque tester having a depth of about 1mm is provided with a test object of about 0.020 to 0.025mL (g) by a closed plaque test method. The patch tester was applied to the forearm of the subject, the test substance was removed after 24 hours, and skin reactions were observed at 0.5, 24, and 48 hours after the removal, and the results were recorded according to the skin reaction classification standard in cosmetic safety technical Specification 2015.

4. The evaluation criteria are shown in Table 5 below:

TABLE 5 skin reaction grading criteria

The results show that: the results of the human skin patch test showed that the score levels of 3 observation time points 0.5h, 24h and 48h after patch removal were all 0 points for 30 subjects. According to the specification of cosmetic safety technology (2015 edition), the test object does not cause adverse skin reaction to the subjects in the batch.

Synergistic test example 4

The proportions of the raw materials of the anti-aging cream compositions of comparative examples 1 to 7 are shown in Table 6 below.

TABLE 6 raw material composition ratios (unit: mass%) of comparative examples 1 to 7

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The preparation process flow of the anti-aging effect creams of comparative examples 1 to 7 is as follows:

4) Sequentially adding the phase D raw materials, and stirring uniformly.

Test method of eye creams of example 1 and comparative examples 1 to 7:

1. the tester: 80 healthy people (20 to 55 years old), women, no obvious redness, skin damage, scar and the like on the face; randomly dividing into 8 groups of 10 persons each, using example 1 and comparative examples 1-7, respectively;

2. test part: full face;

3. test instrument: primos is used for facial image acquisition and wrinkle analysis.

4. The test indexes are as follows: skin wrinkle Ra value; rate of change = (post-use measurement-pre-use measurement)/(pre-use measurement x 100%;

5. the test results are shown in table 7 below:

the anti-aging effect test was conducted on example 1 and comparative examples 1 to 7, and the test results are shown in FIG. 4 and tables 7 to 8.

Table 7 comparison of the Ra values of the skin before and after use of example 1 with comparative examples 1-7

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TABLE 8 comparison of the rate of change of the Ra values of the skin before and after use of example 1 and comparative examples 1-7

	D7	D14	D28	D56
					Example 1	-5.18％	-13.13％	-19.07％	-23.53％
Comparative example 1	-3.45％	-6.07％	-9.55％	-14.12％
					Comparative example 2	-1.80％	-2.31％	-3.43％	-5.65％
Comparative example 3	-1.86％	-2.54％	-3.62％	-4.98％
					Comparative example 4	-1.72％	-2.18％	-3.13％	-4.29％
Comparative example 5	-0.80％	-0.95％	-1.08％	-1.87％
					Comparative example 6	-0.84％	-1.01％	-1.19％	-1.94％
Comparative example 7	-1.00％	-1.23％	-1.56％	-2.27％

Example 1 and comparative examples 1-9 were ordered by skin wrinkle Ra change values before and after use: example 1> comparative example 1>3>2>4>7>6>5, the larger the skin Ra change value, the more pronounced the skin wrinkle lightening effect.

The results show that compared with the results of the control example, the facial cream composition with the synergistic effect liposome has remarkable anti-aging effect, the liposome effect composition has better effect than the effect composition, and simultaneously has better effect than two or single effect components, so that the liposome components in the embodiment of the invention have the effect of promoting the absorption of skin to the effect components and can realize synergistic effect.

The facial anti-aging effect composition consists of bacillus licheniformis/glutamic acid fermentation filtrate, bird's nest acid and procyanidine, and the anti-aging effect route of each component is analyzed: the nidus Collocaliae acid in the composition can supplement nutrition for cells, promote formation of cell membrane glycoprotein and glycolipid, and simultaneously the Bacillus licheniformis/glutamic acid fermentation filtrate can maintain skin moisture, improve dry skin, reduce water deficiency and form fine lines, and also has effects of leveling cutin, promoting metabolism, and maintaining skin microecological balance, thereby enhancing skin barrier function. Furthermore, the procyanidine has strong antioxidant and free radical scavenging effects, can reduce oxidative damage of skin, and can stimulate collagen formation at dermis level to maintain skin smooth and elastic. Meanwhile, the procyanidine has the effect of inhibiting elastase, and can assist the organism to protect collagen and improve skin elasticity, and improve skin health circulation, thereby avoiding or reducing wrinkles.

The above active ingredients are synergistic, and the liposome form allows the active ingredients to act through the skin surface layer to enhance absorption, thereby achieving effective anti-aging effect.

In conclusion, the anti-aging efficacy composite has remarkable wrinkle-removing and anti-aging effects through the synergistic effect of the components, and the efficacy liposome has more remarkable anti-aging effects than the efficacy composite and the combination of two or single components.

The foregoing description is only a preferred embodiment of the present invention and is not intended to limit the invention in any way, so any simple modification, equivalent variation and modification made to the above embodiment according to the technical matter of the present invention will still fall within the scope of the technical scheme of the present invention.

Claims

1. A cream composition having synergistic anti-aging efficacy liposomes, characterized in that: based on the total weight of the composition, the composition consists of the following raw materials:

0.3-35.0% of anti-aging efficacy liposome;

5.0-40.0% emollient;

0.5-30.0% of an emulsifier;

0.5-20.0% filler;

0.1-10.0% of a thickener;

0.3-5.0% preservative;

0.01-1.0% of daily essence and the balance of deionized water;

the anti-aging efficacy liposome comprises: bacillus licheniformis/glutamic acid fermentation filtrate, nidulanic acid, procyanidine and lecithin, wherein the mass ratio of the bacillus licheniformis/glutamic acid fermentation filtrate to the procyanidine to the nidulanic acid to the lecithin is (0.5-8.0): 1: (0.5-6.0): (1-15).

2. A cream composition having synergistic anti-aging efficacy liposomes as claimed in claim 1, wherein: based on the total weight of the composition, the composition consists of the following components:

1-20.0% of anti-aging liposome

10.0 to 25.0 percent of emollient;

5-25.0% of emulsifying agent;

1-15.0% of filler;

0.5-8.0% of thickener;

0.3-1.0% of preservative;

0.01-0.05% of daily essence;

the balance of deionized water was added to 100.0%.

3. A cream composition having synergistic anti-aging efficacy liposomes as claimed in claim 1, wherein: the anti-aging efficacy liposome comprises:

4. A cream composition having synergistic anti-aging efficacy liposomes as claimed in claim 1, wherein: the anti-aging efficacy liposome comprises:

5. A cream composition having synergistic anti-aging efficacy liposomes as claimed in claim 1, wherein: the anti-aging efficacy liposome comprises:

bacillus licheniformis/glutamic acid fermentation filtrate 0.5-10%, nidus Collocaliae acid 0.5-10% and procyanidine 0.5-10%.

6. A cream composition having synergistic anti-aging efficacy liposomes as claimed in claim 1, wherein: the anti-aging efficacy liposome comprises:

7. A cream composition with synergistic anti-aging efficacy liposomes as claimed in any one of claims 1 to 6, wherein: the anti-aging efficacy liposome is prepared by the following process:

5) Ultrasonic treatment is carried out for 5-20min after the membrane is removed completely, and the anti-aging liposome with uniform particle size is obtained after filtration.

8. The cream composition of claim 7, wherein the liposome has synergistic anti-aging effect, and is characterized by: the mass ratio of the phospholipid to the lecithin is 1:1, a step of;

the volume ratio of the water phase to the oil phase is 1: (2-5);

the particle size of the obtained liposome with anti-aging effect is 0.1-0.5 μm.

9. A cream composition with synergistic anti-aging efficacy liposomes as claimed in any one of claims 1 to 6, wherein:

the emollient is selected from at least one of caprylic capric triglyceride, squalane, avocado oil, shea butter, jojoba oil, glycerol, panthenol, shea butter, guaifenesin, tocopheryl acetate, butylene glycol, 1, 2-hexanediol, 4-t-butylcyclohexanol, pentylene glycol, cyclohexanol, trehalose, xylitol, sodium hyaluronate, polydimethylsiloxane, dimethiconol, PEG-11 methyl ether dimethiconol, phytosterol, hydrogenated polyisobutene, rapeseed oil, polydecene, soybean oil, olive oil, lactic acid, arginine, stearyl alcohol, and behenyl alcohol;

the emulsifier is selected from at least one of olive oil PEG-7 esters, PEG-8 caprylic/capric glycerides, cholesterol, sorbitan olive oleate, polysorbate-20, triethanolamine, octyldodecanol, glycerol tri (ethylhexanoate), C12-18 alkyl glucoside, laureth-23, a mixture of glycerol stearate and PEG-100 stearate, steareth-21, palmitols and a mixture of PEG-75 stearate and glycerol stearate and steareth-20, polysorbate-80, a mixture of glyceryl stearate and PEG-75 stearate, poloxamer 407, PEG-30 dimer hydroxystearate, cetostearyl polyether-25;

the filler is at least one selected from titanium dioxide, mica, silica, boron nitride, maltodextrin, polymethylsilsesquioxane, diatomite, kaolin, cellulose acetate, starch octyl aluminum succinate, talcum powder, barium sulfate and volcanic ash;

the thickener is at least one selected from hydroxyethyl cellulose, xanthan gum, cetyl alcohol, carbomer, acrylic acid (ester) copolymer, polymer EMT-10, polyacrylate crosslinked polymer-6, sodium acrylate/sodium acryloyldimethyl taurate copolymer, acrylic ester/C10-30 alkyl acrylate crosslinked polymer, acryloyldimethyl taurate amine/VP copolymer, acrylic acid (ester) type/C10-30 alkanol acrylic ester crosslinked polymer, polyethylene and gum arabic;

the preservative is at least one selected from the group consisting of p-hydroxyacetophenone, phenoxyethanol, ethylhexyl glycerol, hexanediol, benzoyl ketone, sodium benzoate, triclosan and chlorphenicol.

10. A method for preparing a cream composition comprising liposomes having synergistic anti-aging effects as claimed in any one of claims 7 to 9,

the method comprises the following process steps:

4) Sequentially adding the phase D raw materials, and uniformly stirring;

the raw material composition of the face cream composition comprises: the phase A raw material comprises the following components: 0.1-10.0% of a thickener; 5.0-20.0% emollient; 0.3-5.0% preservative; deionized water; phase B raw materials: 0-10.0% emollient; 0.4-20% of an emulsifier; c phase raw materials: 0.1-10% of an emulsifier; the phase D is composed of 0.3-35.0% of anti-aging liposome, 0.01-1.0% of daily essence, 0.5-20.0% of filler, and 0-10.0% of emollient.