CN114099410A

CN114099410A - Face cream composition with liposome with synergistic anti-aging effect and preparation method thereof

Info

Publication number: CN114099410A
Application number: CN202111397927.XA
Authority: CN
Inventors: 王勇; 周紫燕; 杨帆; 祝希宇; 郭苗; 周正
Original assignee: Hubei Maishite Biotechnology Co Ltd
Current assignee: Hubei Maishite Biotechnology Co Ltd
Priority date: 2021-11-23
Filing date: 2021-11-23
Publication date: 2022-03-01
Anticipated expiration: 2041-11-23
Also published as: CN114099410B

Abstract

The invention discloses a face cream composition of liposome with synergistic anti-aging effect and a preparation method thereof. The anti-aging cream composition comprises the following raw material components: 0.3-35.0% of synergistic anti-aging efficacy liposome, 5.0-40.0% of emollient, 0.5-30.0% of emulsifier, 0.5-20.0% of filler, 0.1-10.0% of thickening agent, 0.3-5.0% of preservative, 0.01-1.0% of daily essence and the balance of deionized water. The anti-aging functional component is composed of 0.1-30% of bacillus licheniformis/glutamic acid fermentation filtrate, 0.1-30% of cubilose acid and 0.1-30% of procyanidine, and the functional component is prepared into a lipid inclusion body, so that the activity of the functional component in a formula is protected, and the lipid inclusion body can better permeate a sebum membrane to promote the absorption of skin cells on the functional component, and the anti-aging effect is effectively exerted.

Description

Face cream composition with liposome with synergistic anti-aging effect and preparation method thereof

Technical Field

The invention relates to a cosmetic composition and a preparation method thereof, belongs to the technical field of daily chemicals, and particularly relates to a lipid inclusion facial cream composition containing multiple anti-aging components and having an anti-aging effect by synergistic effect and a preparation method thereof.

Background

The skin of a human body generally ages from 25 to 30 years, namely, the skin gradually ages with the age, and the aging changes are more obvious after about 35 to 40 years. Skin aging is a complex process with a combination of factors, and can be classified into intrinsic aging and extrinsic aging according to the different aging causes.

Endogenous aging, also known as natural aging, is primarily related to the following factors:

the permeability of the stratum corneum is increased, the barrier function of the skin is reduced, the moisture content in the stratum corneum is reduced, and the skin is in a water-deficient state;

the function of the skin accessory organs is reduced, for example, the secretion function of sweat glands and sebaceous glands is gradually weakened along with the increase of age, so that the amount of sweat and sebum secreted is reduced, and the skin cannot be nourished and dried for a long time;

the metabolism speed of the skin is reduced, so that the functions of elastic fibers and collagen fibers in the dermis are reduced, the regulation effect of the skin tension and elasticity is weakened, and the skin is easy to wrinkle;

fourthly, the skin can not absorb sufficient nutrition, so that the storage of subcutaneous fat is continuously reduced, the lower part of the reticular layer of the dermis loses the support, and the skin is loosened.

Exogenous aging refers to skin aging caused by external factors such as ultraviolet radiation, smoking, wind and sun exposure and exposure to toxic and harmful chemicals, and the damage of ultraviolet rays in sunlight to skin is manifold, most importantly, the change of fibrin and matrix components in dermis is caused, and finally, the skin is dry, loose and inelastic.

Aging is a natural law of human growth and cannot be artificially prevented, but the reason of skin aging can be analyzed to pertinently supplement moisture, nutrition and resist external injury to the skin, so that the skin aging is effectively delayed, and the skin has a young and healthy skin state.

However, the existing anti-aging products on the market have various defects of unobvious effect or long effective period, poor permeability and the like, so that the cream skin care product which is easier to absorb, better in anti-aging effect and safer is provided, and is a technical problem which needs to be solved urgently by technical personnel in the field at present.

Disclosure of Invention

The invention aims to provide a cream composition of an anti-aging liposome, which contains various anti-aging functional components and can synergistically increase the anti-aging effect, and the cream composition solves the problems in the background art.

The invention also aims to provide a preparation method of the cream composition of the liposome with the synergistic anti-aging effect.

The technical scheme adopted by the invention is as follows.

The cream composition of the liposome with the synergistic anti-aging effect comprises the following raw material components in percentage by weight of the total weight of the composition:

0.3-35.0% of anti-aging liposome;

5.0-40.0% of an emollient;

0.5-30.0% of emulsifier;

0.5-20.0% of a filler;

0.1-10.0% of a thickening agent;

0.3-5.0% of preservative;

0.01-1.0% of daily essence;

the balance of deionized water.

Further, the cream composition of the liposome with synergistic anti-aging effect comprises the following components by weight:

further, the anti-aging liposome comprises the following raw material components:

0.1-30% of Bacillus licheniformis/glutamic acid fermentation filtrate, 0.1-30% of cubilose acid and 0.1-30% of procyanidine.

0.1-10% of Bacillus licheniformis/glutamic acid fermentation filtrate, 0.1-10% of cubilose acid and 0.1-10% of procyanidine.

bacillus licheniformis/glutamic acid fermentation filtrate 0.5-10%, nidulanic acid 0.5-10% and procyanidin 0.5-10%

0.5-5% of Bacillus licheniformis/glutamic acid fermentation filtrate, 0.5-5% of cubilose acid and 0.5-5% of procyanidine.

the anti-aging efficacy liposome comprises the following raw material components:

5-20% of bacillus licheniformis/glutamic acid fermentation filtrate, 5-25% of cubilose acid and 4-15% of procyanidine.

Further, the emollient is selected from at least one of caprylic capric triglyceride, squalane, avocado oil, shea butter, jojoba oil, glycerin, panthenol, shea butter, guaiol, tocopheryl acetate, butylene glycol, 1, 2-hexanediol, 4-t-butylcyclohexanol, pentanediol, cyclohexanol, trehalose, xylitol, acetylated sodium hyaluronate, polydimethylsiloxane, dimethiconol, PEG-11 methyl ether dimethicone, phytosterol/octyldodecyl lauroyl glutamate, hydrogenated polyisobutene, rapeseed oil, polydecene, soybean oil, olive oil, lactic acid, arginine, stearyl alcohol, and behenyl alcohol.

The emulsifier is selected from at least one of PEG-7 esters of olive oil, glyceryl PEG-8 caprylate/caprate, cholesterol, sorbitan olive oleate, polysorbate-20, triethanolamine, octyldodecanol, glyceryl tri (ethylhexanoate), triethoxyoctylsilane, C12-18 alkylglucoside, laureth-23, a mixture of glyceryl stearate and PEG-100 stearate, steareth-21, palmityl alcohol and PEG-75 stearate and a mixture of glyceryl stearate and steareth-20, polysorbate-80, lecithin, phospholipids, a mixture of glyceryl stearate and PEG-75 stearate, poloxamer 407, PEG-30 dipolyhydroxystearate, and ceteareth-25. The filler is at least one selected from titanium dioxide, mica, silica, boron nitride, maltodextrin, polymethylsilsesquioxane, diatomite, kaolin, cellulose acetate, aluminum starch octylsuccinate, talcum powder, barium sulfate and volcanic ash. The thickening agent is at least one selected from hydroxyethyl cellulose, xanthan gum, cetyl alcohol, carbomer, acrylic acid (ester)/copolymer, polymer EMT-10, polyacrylate cross-linked polymer-6, sodium acrylate/sodium acryloyldimethyl taurate copolymer, acrylic ester/C10-30 alkyl acrylate cross-linked polymer, acryloyldimethyl taurate/VP copolymer, acrylic acid (ester)/C10-30 alkanol acrylate cross-linked polymer, polyethylene and Arabic gum. The antiseptic is at least one selected from p-hydroxyacetophenone, phenoxyethanol, ethylhexyl glycerol, hexanediol, jasmone, sodium benzoate, triclosan and chlorphenesin.

Further, the preparation method of the liposome with synergistic anti-aging effect comprises the following process steps:

1) dissolving phospholipid and lecithin in ethanol, and shaking to dissolve until the solution is transparent to obtain oil phase membrane material solution;

2) decompressing the membrane material solution obtained in the step 1) at the constant temperature of 35 ℃ to remove ethanol until a layer of transparent film is formed on the inner side of the bottle wall, and drying for 10min to obtain a dried substance;

3) dissolving the Bacillus licheniformis/glutamic acid fermentation filtrate, the bird's nest acid and the procyanidine in a phosphate buffer solution with the pH value of 6.8 to prepare an aqueous phase active phosphate buffer solution;

4) adding the active phosphate buffer solution obtained in the step 3) into the dried substance obtained in the step 2), and carrying out normal-pressure rotation oscillation at 35 ℃ to hydrate and remove the membrane;

5) ultrasonic treating for 5-20min after demoulding, and filtering to obtain anti-aging effective component liposome with uniform particle diameter (0.1-0.5 μm).

Preferably, the mass ratio of phospholipid to lecithin is 1: 1; the mass ratio of the bacillus licheniformis/glutamic acid fermentation filtrate to the procyanidine to the bird's nest acid to the lecithin is (0.5-8.0): 1: (0.5-6.0): (1-15); the volume ratio of the water phase to the oil phase is 1: (2-5).

Specifically, the particle size of the liposome with the synergistic anti-aging effect is 0.1-0.5 μm.

In addition, the invention also discloses a preparation method of the cream composition with the liposome with the synergistic anti-aging effect, which comprises the following process steps:

1) sequentially adding the phase A raw materials into a main tank, stirring, mixing and dissolving uniformly, and heating to 80 ℃;

2) sequentially adding the grease of phase B into an oil phase pot, heating to 80 ℃ until all substances are completely dissolved, adding the phase B into the phase A for emulsification and homogenization, and homogenizing for 3 minutes at 3000 r/min;

3) c-phase raw materials are sequentially added, homogenized for 3 minutes at 3000 r/min, and then stirred and cooled to 40 ℃;

4) sequentially adding the D-phase raw materials, and uniformly stirring;

the cream composition comprises the following raw materials:

phase A raw materials: 0.1-10.0% of a thickening agent; 5.0-20.0% of an emollient; 0.3-5.0% of preservative; deionized water;

b phase raw materials: 0-10.0% of an emollient; 0.4-20% of emulsifier;

c phase raw material: 0.1-10% of emulsifier;

the phase D is composed of 0.3-35.0% of anti-aging liposome, 0.01-1.0% of daily essence, 0.5-20.0% of filler, and 0-10.0% of emollient.

In particular, the cream composition of the present invention having liposomes with synergistic anti-aging effects has a pH in the range of 4 to 10, preferably a pH in the range of 5 to 8, and most preferably a pH in the range of 5 to 7.

Specifically, the cream composition of the synergistic anti-aging efficacy liposome can be in a solvent dispersion or suspension mode, can also be in the form of emulsified particles, or can be in a polymer-encapsulated mode.

Specifically, the cream composition of the synergistic anti-aging efficacy liposome can be further designed into other skin care product dosage forms, such as emulsion, essence, cream, eye cream, body lotion, gel and sun block lotion.

By means of the technical scheme, the invention has the following advantages and beneficial technical effects:

1) the invention contains bacillus licheniformis/glutamic acid fermentation filtrate, procyanidine and bird's nest acid, and a plurality of cosmetic raw materials which are rich in nutrition, can replenish water, preserve moisture, resist wrinkle, resist radiation and the like, can enable the skin to become young, smooth and elastic, and can effectively delay skin aging.

2) The anti-aging functional component composition preferably selects bacillus licheniformis/glutamic acid fermentation filtrate, procyanidine and cubilose acid, and the cosmetic passes the preferred combination and efficacy test, selects the most preferred composition proportion and plays an anti-aging role.

3) The anti-aging functional component composition is added into cosmetics in the form of liposome, and the water-soluble functional components are wrapped in the lipid membrane, so that the anti-aging functional components penetrate through skin barriers, and the effects of transdermal absorption and slow release are better achieved.

4) On one hand, the moisturizing capability of the functional composition is utilized, the skin water retention is enhanced, the dry skin is improved, fine wrinkles of the skin caused by water shortage are reduced, and the skin aging is delayed; on the other hand, the composition is used for supplementing nutrition to the skin, promoting the synthesis of collagen, inhibiting elastase, assisting the body to protect the collagen and improving the elasticity of the skin; improving the healthy circulation of the skin and thereby avoiding or reducing the occurrence of wrinkles. In addition, the liposome component promotes the active substances to permeate the stratum corneum of the skin, so that the optimal effect is achieved, and the aim of comprehensive anti-aging is fulfilled.

5) Compared with most of cosmetics sold on the market, the anti-aging liposome face cream provided by the embodiment of the invention has the advantages that the added functional components are mutually cooperated, the safe and effective anti-aging effect is achieved, and the synergistic effect is obvious. The facial cream is suitable for more people with different skin types, and has wide application range and obvious anti-aging effect.

6) The innovative cosmetic with the anti-aging effect can reduce the phenomenon of fine wrinkles on the face, and provides a plurality of ideal benefits for face care so as to bring younger faces.

Drawings

FIG. 1 shows the rate of change of the wrinkle area of the skin before and after the use of example 1 and the blank;

FIG. 2 shows the rate of change of Ra value of skin wrinkles before and after using example 1 and a blank;

FIG. 3 shows the rate of change of the clinical score of skin wrinkles before and after using example 1 and a blank;

FIG. 4 shows the rate of change in Ra value of skin wrinkles before and after use of example 1 and comparative examples 1 to 7.

Detailed Description

The invention discloses a face cream composition of liposome with synergistic anti-aging effect and a preparation method thereof. The anti-aging facial cream comprises the following raw material components:

0.3-35.0% of anti-aging effect liposome, 5.0-40.0% of emollient, 0.5-30.0% of emulsifier, 0.5-20.0% of filler, 0.1-10.0% of thickening agent, 0.3-5.0% of preservative, 0.01-1.0% of daily essence and the balance of deionized water.

The anti-aging effective component is composed of effective liposome prepared from Bacillus licheniformis/glutamic acid fermentation filtrate, nidus Collocaliae acid and procyanidine.

The bacillus licheniformis/glutamic acid fermentation filtrate is rich in amino acid, namely an important component of natural moisturizing factors, and can enhance the content of the natural moisturizing factors in the skin, supplement water instantly, enhance the water retention capacity of the skin, improve the dry skin and ensure that the skin is extremely moist; the skin care product is rich in organic acid, can maintain the acidic environment of the skin, can smooth cutin, promote metabolism and maintain healthy skin; contains polysaccharide prebiotic components, helps the growth of beneficial bacteria, effectively inhibits harmful bacteria, and maintains the microecological balance of skin.

The bird's nest acid is one of eight essential nutrients for human body, and its main source is bird's nest and breast milk. Since the bird's nest is rich in the acid, it is called bird's nest acid. The bird's nest acid is widely distributed in human tissues, exists in the forms of cell membrane glycoprotein, glycolipid and the like, has various biological functions, and has the effects of brightening the skin, resisting aging, resisting oxidation, resisting pigmentation and the like.

Procyanidins are a general term for a large group of polyphenols that are widely present in plants, and are present in the skin, shell, seeds, core, flowers, and leaves of many plants. The procyanidin has strong antioxidant and free radical scavenging effects, can effectively scavenge superoxide anion free radical and hydroxyl free radical, and protect lipid from peroxidation damage, and is a powerful metal chelating agent capable of chelating metal ions and also beneficial to absorption and utilization of vitamin C. The product can be used in cosmetics for eliminating edema; the collagen fibers are helped to form a cross-linked structure, the collagen activity is recovered, and the skin is smooth and elastic; sun screening and whitening; astringing and moisturizing; has multiple effects of resisting radiation.

The components with the anti-aging effects mutually cooperate, and various active ingredients penetrate through the skin surface barrier and permeate to the deep layer, so that moisture and nutrition are supplied to the skin, the activity of collagen is enhanced, and the anti-wrinkle anti-aging effects are exerted.

The present invention is illustrated by the following examples, which are provided for the purpose of further illustration only and are not intended to limit the scope of the present invention. Other insubstantial modifications and adaptations of the present invention can be made without departing from the scope of the present invention. Unless otherwise specified, the following percentages (%) are mass percentages. The materials used in the present invention are all commercially available products.

Examples 1 to 4 and the blank example

The cream composition of the liposome with the synergistic anti-aging effect comprises the following raw material components in percentage by weight and the raw material components in the blank case in percentage by weight, as shown in the following table 1.

Table 1 raw material component ratios (unit: mass%) of examples 1 to 4 and the blank example

The preparation process flow of the cream composition of the liposome with the synergistic anti-aging effect provided by the embodiment of the invention is as follows:

4) and (4) sequentially adding the D-phase raw materials, and uniformly stirring.

Examples 1-4 cream compositions of liposomes having synergistic anti-aging effects prepared have a pH in the range of 5-6.

The preparation method of the liposome in the cream composition containing the liposome with synergistic anti-aging effect of example 1 comprises the following process steps:

1) dissolving 1500mg of phospholipid and 1500mg of lecithin in 100mL of ethanol, shaking for dissolving until the solution is transparent, and preparing an oil phase membrane material solution;

2) decompressing the membrane solution obtained in the step 1) at a constant temperature of 35 ℃ to remove ethanol until a layer of transparent film is formed on the inner side of the bottle wall, and drying for 10 min;

3) dissolving 1500mg of cubilose acid, 300mg of procyanidine and 1200mg of bacillus licheniformis/glutamic acid fermentation filtrate in 30mL of phosphate buffer solution with pH of 6.8 to obtain water-phase active phosphate buffer solution;

4) adding the active phosphate buffer solution in the step 3) into the dried substance obtained in the step 2), and performing normal-pressure rotary oscillation hydration and demoulding at the temperature of 35 ℃;

5) and (3) carrying out ultrasonic treatment for 5-20min after complete membrane removal, and filtering to obtain the liposome with uniform particle size (0.18 +/-0.03 mu m) and anti-aging effect.

The preparation method of the liposome in the cream composition containing the liposome with synergistic anti-aging effect of the embodiment 2 comprises the following process steps:

1) dissolving 2700mg of phospholipid and 2700mg of lecithin in 120mL of ethanol, and shaking for dissolving until the solution is transparent to obtain an oil-phase membrane material solution;

3) dissolving 1200mg of cubilose acid, 1050mg of procyanidine and 750mg of bacillus licheniformis/glutamic acid fermentation filtrate in 30mL of phosphate buffer solution with pH6.8 to prepare water-phase active phosphate buffer solution;

5) and (3) carrying out ultrasonic treatment for 5-20min after complete demoulding, and filtering to obtain the liposome with uniform particle size (0.16 +/-0.05 mu m) and the anti-aging effect.

The preparation method of the liposome in the cream composition containing the liposome with synergistic anti-aging effect of the embodiment 3 comprises the following process steps:

1) dissolving 3300mg of phospholipid and 3300mg of lecithin in 120mL of ethanol, shaking for dissolving until the solution is transparent, and preparing into oil phase membrane material solution;

3) dissolving 750mg of cubilose acid, 450mg of procyanidine and 1800mg of bacillus licheniformis/glutamic acid fermentation filtrate in 30mL of phosphate buffer solution with pH6.8 to prepare water-phase active phosphate buffer solution;

5) and (3) carrying out ultrasonic treatment for 5-20min after complete demoulding, and filtering to obtain the liposome with uniform particle size (0.15 +/-0.05 mu m) and the anti-aging effect.

The preparation method of the liposome in the cream composition containing the liposome with synergistic anti-aging effect of the embodiment 4 comprises the following process steps:

1) dissolving 3900mg of phospholipid and 3900mg of lecithin in 150mL of ethanol, shaking for dissolving until the solution is transparent, and preparing an oil-phase membrane material solution;

3) dissolving 1350mg of cubilose acid, 750mg of procyanidin and 900mg of bacillus licheniformis/glutamic acid fermentation filtrate in 30mL of phosphate buffer solution with pH of 6.8 to prepare water-phase active phosphate buffer solution;

5) and (3) carrying out ultrasonic treatment for 5-20min after complete demoulding, and filtering to obtain the liposome with uniform particle size (0.13 +/-0.05 mu m) and the anti-aging effect.

Test example for Effect verification

Test 1 for anti-aging and wrinkle-reducing effects

1. The tester: 60 healthy people (20-55 years old) and women are randomly divided into two groups, wherein one group uses the example 1, and the other group uses the blank; the face has no obvious redness, skin damage, scars and other conditions;

2. testing parts: a full face;

3. testing an instrument: Visia-CR was used for facial image acquisition and wrinkle analysis. Primos was used for facial image acquisition and wrinkle analysis.

4. Testing indexes are as follows: the rate of change of the area of skin wrinkles, the rate of change of the Ra values of skin wrinkles; change rate ═ (after use measure-before use measure) ÷ before use measure × 100%;

5. the test results are shown in FIGS. 1-2 and tables 2-3 below:

TABLE 2 comparison of skin wrinkle area ratio

	D0	D7	D14	D28	D56
						Example 1	2.44	2.39	2.10	1.86	1.62
Blank example	2.38	2.38	2.35	2.31	2.30

TABLE 3 skin wrinkle Ra values

	D0	D7	△D14	△D28	△D56
						Example 1	23.86	22.61	20.79	19.33	18.24
Blank example	23.75	23.67	23.61	23.56	23.58

Skin wrinkles facial image acquisition and wrinkle analysis were performed with Visia-CR and Primos. Skin wrinkle area ratios of five time points of D0, D7, D14, D28, and D56 measured according to Visia-CR (table 2), showed that the skin wrinkle area was reduced by 2.05% after 7 days of use compared to before use after use of example 1; after 14 days of use, the skin wrinkle area was reduced by 13.93%; after 28 days of use, the wrinkle area was reduced by 23.77%; after 56 days of use, the wrinkle area was reduced 33.61%. Compared with the results of the blank case, the wrinkle area rate change rate of the example 1 is significantly different after 7 days of use; after 14 days of use, the wrinkle area rate change rate of the embodiment 1 has a significant difference; after 28 days of use, the wrinkle area rate change rate of the embodiment 1 has a significant difference; after 56 days of use, there was a significant difference in the rate of change of the wrinkle area ratio in example 1.

Skin wrinkle Ra values at five time points D0, D7, D14, D28, D56 measured according to Primos (table 3), the results showed that the skin wrinkle Ra value was reduced by 5.24% after 7 days of use compared to before use after use of example 1; after 14 days of use, the Ra value of skin wrinkles was reduced by 12.87%; after 28 days of use, the wrinkle Ra value decreased 18.99%; after 56 days of use, the wrinkle Ra value decreased 23.55%. Compared with the results of the blank case, the change rate of the Ra value of the wrinkles in the example 1 is obviously different after 7 days of use; after 14 days of use, the change rate of Ra value of the wrinkles in the example 1 is obviously different; after 28 days of use, the change rate of the Ra value of the wrinkles in the example 1 is obviously different; after 56 days of use, the change rate of the Ra value of the wrinkles in example 1 was significantly different.

In conclusion, the embodiment of the invention contains the anti-aging liposome, and has obvious anti-wrinkle and anti-aging effects.

Clinical evaluation test 2 for dermatologists

2. testing parts: a full face;

4. Evaluation criteria: the dermatologist performs wrinkle assessment from the captured images: score 0 indicates no wrinkles and score 6 indicates very deep wrinkles.

5. And (3) testing results: as shown in table 4 and fig. 3.

TABLE 4 mean wrinkle clinical score of two groups of subjects

	D0	D7	D14	D28	D56
						Example 1	2.34	2.21	2.07	1.88	1.69
Blank example	2.28	2.28	2.25	2.23	2.21

The results show that the clinical score of skin wrinkles after using example 1 was reduced by 5.56% compared to before using, after 7 days; after 14 days of use, the clinical score of skin wrinkles decreased by 11.54%; after 28 days of use, the wrinkle clinical score decreased by 19.66%; after 56 days of use, the clinical score of wrinkles decreased by 27.78%. Compared with the results of the blank case, after 7 days of use, the change rate of the wrinkle clinical score value in example 1 is significantly different; after 14 days of use, the change rate of the wrinkle clinical score value in example 1 is significantly different; after 28 days of use, the change rate of the wrinkle clinical score value in example 1 is significantly different; after 56 days of use, the change rate of the wrinkle clinical score value in example 1 was significantly different.

In conclusion, the embodiment of the invention has obvious anti-wrinkle effect, and wrinkles are one of the characteristics of skin aging, so that the embodiment of the invention can effectively delay the skin aging.

Security verification 3

1. The number of tested persons: 30 healthy persons (20-55 years old) were tested with sample 1 formulation.

2. Testing parts: the curved side of the forearm;

3. the test method comprises the following steps: the selection area is not more than 50mm²And (g) about 0.020 to 0.025mL (g) of a test substance in a closed patch test method in a suitable patch tester having a depth of about 1 mm. The patch tester is applied to the curved side of the forearm of the subject, the subject is removed 24 hours later, the skin reaction is observed 0.5 hour, 24 hours and 48 hours after the removal, and the result is recorded according to the skin reaction grading standard in the technical Specification for cosmetic safety 2015.

4. The evaluation criteria are as follows in table 5:

TABLE 5 grading Standard of skin reactions

The results show that: the result of the human skin patch experiment shows that the score grades of 0.5h, 24h and 48h of 3 observation time points of 30 subjects after the patch is removed are all 0. The subject did not cause adverse skin reactions to the subjects of this batch as specified in the technical Specification for cosmetic safety (2015 edition).

Synergistic test example 4

The anti-aging cream compositions of comparative examples 1 to 7 were prepared as shown in table 6 below.

TABLE 6 raw material composition ratio (% by mass) of comparative examples 1 to 7

The preparation process of the anti-aging effect face cream of the comparative examples 1 to 7 is as follows:

Test methods of the eye creams of example 1 and comparative examples 1 to 7:

1. the tester: 80 healthy people (20-55 years old) and women have no obvious red, skin damage, scars and other conditions on the face; randomly divided into 8 groups of 10 persons each, using example 1 and comparative examples 1-7, respectively;

2. testing parts: a full face;

3. testing an instrument: primos was used for facial image acquisition and wrinkle analysis.

4. Testing indexes are as follows: skin wrinkle Ra value; change rate ═ (after use measure-before use measure) ÷ before use measure × 100%;

5. the test results are shown in table 7 below:

the anti-aging effect test was performed on example 1 and comparative examples 1 to 7, and the test results are shown in FIG. 4 and tables 7 to 8.

TABLE 7 comparison of Ra values of skin before and after use in example 1 with comparative examples 1-7

TABLE 8 comparison of the rate of change of Ra value in skin before and after use in example 1 with comparative examples 1 to 7

	D7	D14	D28	D56
					Example 1	-5.18％	-13.13％	-19.07％	-23.53％
Comparative example 1	-3.45％	-6.07％	-9.55％	-14.12％
					Comparative example 2	-1.80％	-2.31％	-3.43％	-5.65％
Comparative example 3	-1.86％	-2.54％	-3.62％	-4.98％
					Comparative example 4	-1.72％	-2.18％	-3.13％	-4.29％
Comparative example 5	-0.80％	-0.95％	-1.08％	-1.87％
					Comparative example 6	-0.84％	-1.01％	-1.19％	-1.94％
Comparative example 7	-1.00％	-1.23％	-1.56％	-2.27％

Before and after the use of example 1 and comparative examples 1 to 9, the skin wrinkles Ra change values are ranked as: example 1> comparative example 1>3>2>4>7>6>5, the greater the change in skin Ra, the more pronounced the skin wrinkle lightening effect.

The results show that compared with the results of the control example, the cream composition with the liposome having the synergistic anti-aging effect has obvious anti-aging effect, the liposome efficacy composition has better effect than the efficacy composition, and simultaneously has better effect than two or single efficacy ingredients, and the liposome ingredients in the embodiment of the invention have the effect of promoting the absorption of skin on the efficacy ingredients and can realize synergistic interaction.

The facial anti-aging effect composition consists of bacillus licheniformis/glutamic acid fermentation filtrate, cubilose acid and procyanidine, and the anti-aging action ways of all the components are analyzed as follows: the nidus Collocaliae acid in the composition can supply nutrition for cell, promote the formation of cell membrane glycoprotein and glycolipid, keep skin moisture, improve dry skin, reduce fine lines due to water deficiency, and balance cutin, promote metabolism, and maintain skin microecological balance to enhance skin barrier function. Further, procyanidins have strong antioxidant, free radical scavenging, skin oxidative damage reducing, collagen formation stimulating, skin smoothness and elasticity maintaining effects. Meanwhile, the procyanidine has the effect of inhibiting elastase, and can assist the body to protect collagen, improve the elasticity of the skin and improve the healthy circulation of the skin, so that the generation of wrinkles is avoided or reduced.

The active ingredients are mutually synergistic, and the liposome form enables the active ingredients to act through the surface layer of the skin to enhance absorption, thereby achieving the effective anti-aging effect.

In conclusion, the anti-aging efficacy compound has a relatively obvious wrinkle-removing anti-aging effect through the synergistic effect of the components, and the efficacy liposome has a more obvious anti-aging effect than the efficacy composition and the combination of two or a single component of the efficacy composition.

The above description is only a preferred embodiment of the present invention, and is not intended to limit the present invention in any way, so that any simple modification, equivalent change and modification made to the above embodiment according to the technical spirit of the present invention are within the scope of the technical solution of the present invention.

Claims

1. A face cream composition of liposome with synergistic anti-aging effect is characterized in that: the composition consists of the following raw materials in percentage by weight based on the total weight of the composition:

0.3-35.0% of anti-aging liposome;

5.0-40.0% of an emollient;

0.5-30.0% of emulsifier;

0.5-20.0% of a filler;

0.1-10.0% of a thickening agent;

0.3-5.0% of preservative;

0.01-1.0% of daily essence and the balance of deionized water.

2. The cream composition of liposomes having synergistic anti-aging efficacy as claimed in claim 1, wherein: the composition comprises the following components in percentage by weight based on the total weight of the composition:

the balance of deionized water was added to 100.0%.

3. The cream composition of liposomes having synergistic anti-aging efficacy as claimed in claim 1, wherein: the anti-aging efficacy liposome comprises:

4. The cream composition of liposomes having synergistic anti-aging efficacy as claimed in claim 1, wherein: the anti-aging efficacy liposome comprises:

5. The cream composition of liposomes having synergistic anti-aging efficacy as claimed in claim 1, wherein: the anti-aging efficacy liposome comprises:

0.5-10% of Bacillus licheniformis/glutamic acid fermentation filtrate, 0.5-10% of cubilose acid and 0.5-10% of procyanidine.

6. The cream composition of liposomes having synergistic anti-aging efficacy as claimed in claim 1, wherein: the anti-aging efficacy liposome comprises:

7. The cream composition of liposomes having synergistic anti-aging efficacy as claimed in any one of claims 1 to 6, wherein: the anti-aging liposome is prepared by the following process:

5) and (3) carrying out ultrasonic treatment for 5-20min after complete membrane removal, and filtering to obtain the anti-aging effect liposome with uniform particle size.

8. The cream composition of liposomes having synergistic anti-aging efficacy as claimed in claim 7, wherein:

the mass ratio of the phospholipid to the lecithin is 1: 1;

the mass ratio of the bacillus licheniformis/glutamic acid fermentation filtrate to the procyanidine to the bird's nest acid to the lecithin is (0.5-8.0): 1: (0.5-6.0): (1-15);

the volume ratio of the water phase to the oil phase is 1: (2-5);

the particle size of the prepared anti-aging effect liposome is 0.1-0.5 μm.

9. The cream composition of liposomes having synergistic anti-aging efficacy as claimed in any one of claims 1 to 6, wherein:

the emollient is selected from at least one of caprylic capric triglyceride, squalane, avocado oil, shea butter, jojoba oil, glycerol, panthenol, shea butter, guaiacol, tocopheryl acetate, butylene glycol, 1, 2-hexanediol, 4-tert-butylcyclohexanol, pentanediol, cyclohexanol, trehalose, xylitol, acetylated sodium hyaluronate, polydimethylsiloxane, dimethiconol, PEG-11 methyl ether polydimethylsiloxane, phytosterol/octyldodecyl lauroyl glutamic acid, hydrogenated polyisobutene, rapeseed oil, polydecene, soybean oil, olive oil, lactic acid, arginine, stearyl alcohol, and behenyl alcohol;

the emulsifier is selected from at least one of olive oil PEG-7 esters, PEG-8 caprylic/capric glycerides, cholesterol, sorbitan olive oleate, polysorbate-20, triethanolamine, octyldodecanol, glycerol tri (ethylhexanoate), triethoxyoctylsilane, C12-18 alkylglucoside, laureth-23, glyceryl stearate and PEG-100 stearate mixture, steareth-21, palmityl alcohol and PEG-75 stearate and a mixture of glyceryl stearate and steareth-20, polysorbate-80, lecithin, phospholipids, a mixture of glyceryl stearate and PEG-75 stearate, poloxamer 407, PEG-30 dipolyhydroxystearate, ceteareth-25;

the filler is selected from at least one of titanium dioxide, mica, silica, boron nitride, maltodextrin, polymethylsilsesquioxane, diatomite, kaolin, cellulose acetate, aluminum starch octylsuccinate, talcum powder, barium sulfate and volcanic ash;

the thickening agent is selected from at least one of hydroxyethyl cellulose, xanthan gum, cetyl alcohol, carbomer, acrylic acid (ester)/copolymer, polymer EMT-10, polyacrylate cross-linked polymer-6, sodium acrylate/sodium acryloyldimethyl taurate copolymer, acrylic ester/C10-30 alkyl acrylate cross-linked polymer, acryloyldimethyl taurate/VP copolymer, acrylic acid (ester)/C10-30 alkanol acrylate cross-linked polymer, polyethylene and Arabic gum;

the antiseptic is at least one selected from p-hydroxyacetophenone, phenoxyethanol, ethylhexyl glycerol, hexanediol, jasmone, sodium benzoate, triclosan and chlorphenesin.

10. The preparation method of the cream composition of liposomes with synergic anti-aging effect as claimed in any one of claims 7 to 9, characterized by comprising the following process steps:

4) sequentially adding the D-phase raw materials, and uniformly stirring;

the cream composition comprises the following raw materials: the phase A raw material: 0.1-10.0% of a thickening agent; 5.0-20.0% of an emollient; 0.3-5.0% of preservative; deionized water; b phase raw materials: 0-10.0% of an emollient; 0.4-20% of emulsifier; c phase raw material: 0.1-10% of emulsifier; the phase D is composed of 0.3-35.0% of anti-aging liposome, 0.01-1.0% of daily essence, 0.5-20.0% of filler, and 0-10.0% of emollient.